Fermented cordyceps sobolifera spore powder with effects of dispelling effects of alcohol and protecting liver and preparation method of fermented cordyceps sobolifera spore powder

Abstract

The invention discloses fermented cordyceps sobolifera conidial powder with effects of dispelling effects of alcohol and protecting liver and a preparation method thereof. The fermented cordyceps sobolifera conidial powder is prepared from the following components in percentage by mass: 25-35% of cordyceps polysaccharide; 6%-10% of a nucleoside compound; 3%-7% of ergosterol; 1%-4% of alkaloid; 0.1%-1% of a fermentation component; 7%-15% of auxiliary components; and the balance of other components. According to the preparation method of the fermented cordyceps sobolifera spore powder, the modern fermentation engineering technology and the traditional Chinese medicine theory are combined, the extraction efficiency of active ingredients in the cordyceps sobolifera spore powder is remarkably improved, and the hangover alleviating and liver protecting effects of the product are enhanced by optimizing fermentation parameters; according to the present invention, the advanced gel chromatographic column separation technology and the dialysis method are adopted during the purification process, such that the small molecule impurities are effectively removed, the purity and the safety of the product are improved, the automatic control of the whole production process flow is achieved, the human factor interference is reduced, and the production cost is reduced. And the consistency and reliability between batches are ensured.

Description

Technical Field

[0001] The invention relates to the technical field of active ingredients of Cordyceps sinensis spore powder, and in particular to fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects and a preparation method thereof. Background Art

[0002] Cordyceps sinensis is a traditional Chinese medicine, and its main active ingredients include polysaccharides, nucleoside compounds, ergosterol, etc. It has multiple pharmacological effects, such as immunomodulation, anti-oxidation, anti-fatigue, and protection of renal function. Existing studies have shown that the polysaccharide components in Cordyceps sinensis can not only repair damaged glomeruli, but also show good antioxidant activity, which helps to reduce liver damage and promote liver repair.

[0003] In the specific implementation process, the traditional extraction method has limitations in extraction efficiency and product quality. In order to improve the extraction rate of active ingredients in Cordyceps sinensis spore powder and give the product the effect of sobering up and protecting the liver, it is necessary to develop new extraction technology and process conditions. Therefore, the present invention aims to provide a method for efficiently extracting and preparing a product with the effect of sobering up and protecting the liver from Cordyceps sinensis spore powder through fermentation treatment. Summary of the invention

[0004] The purpose of the present invention is to solve the above problems and to provide a fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects and a preparation method thereof.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects, comprising the following components in percentage by mass:

[0007] Cordyceps polysaccharide 25%-35%;

[0008] Nucleoside compounds 6%-10%;

[0009] Ergosterol 3%-7%;

[0010] Alkaloids 1%-4%;

[0011] Fermentation ingredients 0.1%-1%;

[0012] Auxiliary ingredients 7%-15%;

[0013] The balance is other ingredients

[0014] Preferably, the fermentation ingredients are fermentation strains.

[0015] Preferably, the auxiliary ingredients include:

[0016] Plant extract 5%-10%;

[0017] Nanoparticles 0.01%-0.5%;

[0018] Sea cucumber peptide 2%-5%.

[0019] Preferably, the other ingredients are excipients, and the excipient is microcrystalline cellulose.

[0020] Preferably, the other ingredients further include a preservative, the preservative accounts for 0.1%-0.5% of the total mass, and the preservative is potassium sorbate.

[0021] Preferably, the other ingredients further include flavoring agents, and the flavoring agents account for 0%-2% of the total mass.

[0022] The second object of the present invention is achieved by the following technical scheme: The preparation method of the fermented Cordyceps sinensis spore powder with the effect of sobering up and protecting the liver comprises the following steps:

[0023] S1. Collection and preprocessing:

[0024] S11, selecting high-quality Cordyceps sinensis spore powder as starting material;

[0025] S12, crushing the collected Cordyceps sinensis spore powder to pass through a 100-200 mesh sieve;

[0026] S2. Fermentation treatment:

[0027] The pretreated Cordyceps sinensis spore powder is added to the mixed solvent, and ultrasonic-assisted fermentation is performed at 30-40° C. for 0.5-1 hour, during which a magnetic stirrer is used to maintain uniform stirring, wherein the ultrasonic power is set to 200-400 W, the frequency range is 35-45 kHz, and the stirring speed is controlled at 100-200 r / min;

[0028] S3. Extraction and purification:

[0029] S31, after fermentation, filtering with sterile filter paper to remove solid residues, and then concentrating the extract to 1 / 10 to 1 / 5 of the original volume using a rotary evaporator, then adding 95% ethanol to the concentrate to precipitate polysaccharides, separating the polysaccharide precipitate after standing for 24 hours, and washing with cold alcohol 2 to 3 times to remove impurities;

[0030] S32, subjecting the washed polysaccharide precipitate to gel chromatography column separation, selecting a buffer to dissolve the polysaccharide precipitate, and loading the sample onto the gel chromatography column, using an ultraviolet detector to monitor the elution process, and finally collecting the eluate containing the target polysaccharide, and then placing the obtained eluate into a dialysis bag, and placing it into a Tris-HCl buffer for dialysis treatment, replacing the buffer every 2-4 hours until a 24-48 hour dialysis cycle is completed, thereby obtaining a pure Cordyceps polysaccharide solution;

[0031] S4. Drying and packaging:

[0032] The obtained cordyceps polysaccharide solution is placed in a vacuum freeze dryer for drying, the freezing temperature is set at -40 to -60°C, the drying time is 12 to 36 hours, and the pressure is maintained at 0.1 to 10 Pa. After drying, the product is packaged in a sealed container using aseptic technology and stored in a low temperature environment of 4 to 8°C to ensure the stability and activity of the product.

[0033] Preferably, the mixed solvent consists of distilled water, ethanol, plant extracts and nanoparticles.

[0034] In summary, due to the adoption of the above technical solution, the beneficial effects of the present invention are:

[0035] 1. In the present application, the preparation method of fermented Cordyceps sinensis spore powder combines modern fermentation engineering technology with traditional Chinese medicine theory, significantly improves the extraction efficiency of active ingredients in Cordyceps sinensis spore powder, and enhances the product's alcohol-removing and liver-protecting effects by optimizing fermentation parameters, especially for liver damage caused by long-term drinking.

[0036] 2. In this application, advanced gel chromatography column separation technology and dialysis method were used in the purification process to effectively remove small molecule impurities, improve the purity and safety of the product, and realize automated control of the entire production process, reduce interference from human factors, and ensure consistency and reliability between batches. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 A workflow diagram provided according to an embodiment of the present invention is shown. DETAILED DESCRIPTION

[0038] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] See also Figure 1The present invention provides a technical solution: a fermented Cordyceps sinensis spore powder with the effects of sobering up and protecting the liver, which is composed of the following components in percentage by mass:

[0040] Cordyceps polysaccharide 25%-35%;

[0041] Nucleoside compounds 6%-10%;

[0042] Ergosterol 3%-7%;

[0043] Alkaloids 1%-4%;

[0044] Fermentation ingredients 0.1%-1%;

[0045] Auxiliary ingredients 7%-15%;

[0046] The balance is other ingredients.

[0047] Fermented ingredients Fermented strains use specific probiotics or fermented strains for fermentation treatment, which can activate the enzyme system in the spores, increase the release of active ingredients, and improve the taste and absorption performance of the product.

[0048] Auxiliary ingredients include:

[0049] Plant extracts 5%-10%, including but not limited to kudzu root isoflavones, etc. These ingredients can not only assist the fermentation process, but also provide additional health benefits;

[0050] Nanoparticles 0.01%-0.5%, such as silica nanoparticles or gold nanoparticles, are used as carriers or catalysts to improve fermentation efficiency and utilization of active ingredients;

[0051] Sea cucumber peptide 2%-5%. Sea cucumber peptide is a peptide substance with good alcohol sobering and liver protecting properties, which can further enhance the product's alcohol sobering and liver protecting effects.

[0052] The other ingredients are excipients, which use microcrystalline cellulose, such as lactose, microcrystalline cellulose, etc., to ensure that the physical form of the final product is stable and easy to produce and take; excipients help form tablets or other dosage forms to ensure product quality.

[0053] Other ingredients include preservatives, which account for 0.1%-0.5% of the total weight. Potassium sorbate is used as the preservative. In order to extend the shelf life, an appropriate amount of food-grade preservatives is added when necessary.

[0054] Other ingredients include flavorings, which account for 0%-2% of the total mass. Some flavorings of natural origin may be added to improve the taste, but their use should be minimized to maintain the natural properties of the product.

[0055] A preparation method of fermented cicada flower cordyceps spore powder with the effect of relieving hangover and protecting the liver, comprising the following steps:

[0056] S1. Collection and pretreatment:

[0057] S11. Select high-quality cicada flower cordyceps spore powder as the starting material;

[0058] S12. Crush the collected cicada flower cordyceps spore powder to pass through a 100-200 mesh sieve;

[0059] S2. Fermentation treatment:

[0060] Add the pretreated cicada flower cordyceps spore powder to a mixed solvent, which consists of distilled water, ethanol, plant extract and nanoparticles, and carry out ultrasonic-assisted fermentation at 30-40 °C for 0.5-1 hour. During this period, use a magnetic stirrer to keep stirring at a constant speed. Among them, the ultrasonic power is set at 200-400 W, the frequency range is 35-45 kHz, and the stirring speed is controlled at 100-200 r / min;

[0061] S3. Extraction and purification:

[0062] S31. After fermentation, use a sterile filter paper to filter out solid residues, then use a rotary evaporator to concentrate the extract to 1 / 10-1 / 5 of the original volume. Then add 95% ethanol to the concentrated solution to precipitate polysaccharides. After standing for 24 hours, separate the polysaccharide precipitate, and wash it with cold alcohol 2-3 times to remove impurities;

[0063] S32. Perform gel chromatography column separation on the washed polysaccharide precipitate. Select a buffer solution to dissolve the polysaccharide precipitate, and load it onto the gel chromatography column. Use an ultraviolet detector to monitor the elution process. Finally, collect the eluate containing the target polysaccharide. After that, place the obtained eluate in a dialysis bag and put it into Tris-HCl buffer solution for dialysis treatment. Replace the buffer solution every 2-4 hours until the 24-48 hour dialysis cycle is completed, so as to obtain a pure cordyceps polysaccharide solution;

[0064] S4. Drying and encapsulation:

[0065] Place the obtained cordyceps polysaccharide solution in a vacuum freeze dryer for drying. Set the freezing temperature at -40 to -60 °C, the drying time at 12-36 hours, and the pressure at 0.1-10 Pa. After drying, use aseptic technology to encapsulate the product in a sealed container and store it in a low-temperature environment of 4-8 °C to ensure the stability and activity of the product.

[0066] The above description of the embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects, characterized in that: It is composed of the following components in percentage by mass: Cordyceps polysaccharide 25%-35%; Nucleoside compounds 6%-10%; Ergosterol 3%-7%; Alkaloids 1%-4%; Fermentation ingredients 0.1%-1%; Auxiliary ingredients 7%-15%; The balance is other ingredients.

2. The fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects according to claim 1, characterized in that: The fermentation ingredients are fermentation strains.

3. The fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects according to claim 2, characterized in that: The auxiliary ingredients include: Plant extract 5%-10%; Nanoparticles 0.01%-0.5%; Sea cucumber peptide 2%-5%.

4. The fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects according to claim 3, characterized in that: The other ingredients are excipients, and the excipient is microcrystalline cellulose.

5. The fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects according to claim 4, characterized in that: The other ingredients also include preservatives, which account for 0.1%-0.5% of the total mass, and the preservative is potassium sorbate.

6. The fermented Cordyceps sinensis spore powder with alcohol sobering and liver protecting effects according to claim 5, characterized in that: The other ingredients also include flavoring agents, which account for 0%-2% of the total weight.

7. A method for preparing fermented Cordyceps sinensis spore powder having alcohol sobering and liver protecting effects according to any one of claims 1 to 6, characterized in that: The following steps are involved: S1. Collection and preprocessing: S11, selecting high-quality Cordyceps sinensis spore powder as starting material; S12, crushing the collected Cordyceps sinensis spore powder to pass through a 100-200 mesh sieve; S2. Fermentation treatment: The pretreated Cordyceps sinensis spore powder is added to the mixed solvent, and ultrasonic-assisted fermentation is performed at 30-40° C. for 0.5-1 hour, during which a magnetic stirrer is used to maintain uniform stirring, wherein the ultrasonic power is set to 200-400 W, the frequency range is 35-45 kHz, and the stirring speed is controlled at 100-200 r / min; S3. Extraction and purification: S31, after fermentation, filtering with sterile filter paper to remove solid residues, and then concentrating the extract to 1 / 10 to 1 / 5 of the original volume using a rotary evaporator, then adding 95% ethanol to the concentrate to precipitate polysaccharides, separating the polysaccharide precipitate after standing for 24 hours, and washing with cold alcohol 2 to 3 times to remove impurities; S32, subjecting the washed polysaccharide precipitate to gel chromatography column separation, selecting a buffer to dissolve the polysaccharide precipitate, and loading the sample onto the gel chromatography column, using an ultraviolet detector to monitor the elution process, and finally collecting the eluate containing the target polysaccharide, and then placing the obtained eluate into a dialysis bag, and placing it into a Tris-HCl buffer for dialysis treatment, replacing the buffer every 2-4 hours until a 24-48 hour dialysis cycle is completed, thereby obtaining a pure Cordyceps polysaccharide solution; S4. Drying and packaging: The obtained cordyceps polysaccharide solution is placed in a vacuum freeze dryer for drying, the freezing temperature is set at -40 to -60°C, the drying time is 12 to 36 hours, and the pressure is maintained at 0.1 to 10 Pa. After drying, the product is packaged in a sealed container using aseptic technology and stored in a low temperature environment of 4 to 8°C to ensure the stability and activity of the product.

8. The method for preparing fermented Cordyceps sinensis spore powder having the effects of sobering up and protecting the liver according to claim 7, characterized in that: The mixed solvent consists of distilled water, ethanol, plant extracts and nanoparticles.

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