Composition containing camellia oleifera extract and preparation method thereof

Abstract

The invention belongs to the field of oil tea, and particularly relates to a composition containing an oil tea extract and a preparation method of the composition. According to the composition containing the oil tea extract, the banana flowers and the Yunnan big-leaf sunned raw tea are mixed and subjected to pile fermentation, so that the banana flowers and the Yunnan big-leaf sunned raw tea mutually absorb aroma in the mixing process; in the spreading process, microorganisms in the Yunnan big-leaf sunned raw tea are utilized to realize primary fermentation of active ingredients of the banana flowers, then the camellia oleifera extract is added for continuous sealed storage, and finally the roses are added for aging treatment, so that the inhibitory activity of the composition on advanced glycosylation end products can be improved, and the content of the active ingredients of the banana flowers is increased. Research and development of products with AGEs inhibitory activity are enriched, and a new thought is provided for research and development of AGEs inhibitors.

Description

Technical Field

[0001] The invention belongs to the application field of camellia oleifera extract, and particularly relates to a composition containing the camellia oleifera extract and a preparation method thereof. Background Art

[0002] Advanced glycation endproducts (AGEs) are products of excess sugar and protein. They have two sources in the body: one is the synthesis of AGEs by excess sugar and protein in the body, and the other is the intake of AGEs in food. AGEs can combine with and destroy tissue cells in the body, accelerate the aging of the human body and cause many chronic degenerative diseases. Therefore, research on products that have inhibitory activity against AGEs is of great significance for preventing aging and other health problems. Summary of the invention

[0003] The invention provides a composition containing camellia oleifera extract and a preparation method thereof, which has significant inhibitory activity on advanced glycation end products.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A composition containing camellia oleifera extract comprises the following components in parts by weight: 1-5 parts of camellia oleifera extract, 10-20 parts of banana flowers, 5-15 parts of Yunnan large-leaf sun-dried green tea, and 5-10 parts of rose flowers.

[0006] Furthermore, the composition comprises the following components in parts by weight: 3 parts of camellia oleifera extract, 15 parts of banana flowers, 10 parts of Yunnan large-leaf sun-dried green tea, and 8 parts of rose flowers.

[0007] Furthermore, the banana flowers are freshly picked banana flowers, and the rose flowers are freshly picked rose flowers.

[0008] Furthermore, the preparation method of the camellia oleifera extract comprises the following steps:

[0009] The tea seed cake is defatted and dried to obtain defatted cake, and then water 5-6 times the mass of the defatted cake is added and heated and boiled for 20-30 minutes, insoluble matter is removed to obtain a filtrate, and the filtrate is dried to obtain the product.

[0010] A method for preparing a composition containing a camellia oleifera extract comprises the following steps:

[0011] (1) freshly picked banana flowers and Yunnan large-leaf sun-dried green tea are taken separately, and after removing impurities, washing and drying, the banana flowers are cut into thin strips, and then withered, fixed, rolled, and sun-dried for use;

[0012] (2) taking sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mixing them evenly to obtain a mixture; and placing the mixture in a room for 40-60 days;

[0013] (3) adding water to the camellia oleifera extract to prepare a suspension, adding the suspension to the mixture and mixing evenly to prepare a mixture, then drying to a water content of 20-30%, and stacking in a sealed room away from light for 1-3 days;

[0014] (4) After the sealed storage is completed, dry it; then add dried rose petals, mix well, and age it for at least 30 days.

[0015] Furthermore, in the step (1), the banana flowers are cut or torn into thin strips with a width of 0.5-1.0 cm; the banana flowers and Yunnan large-leaf sun-dried green tea are spread on bamboo mats or bamboo sieves respectively, and placed in a well-ventilated indoor or outdoor shaded place away from direct sunlight to wither until the water content of the banana flowers and the Yunnan large-leaf sun-dried green tea reaches 60-70%; the temperature is controlled at 180-220° C. during the withering process; the banana flowers are rolled until the strips are curled and tightly bound and a little juice oozes out; the sun-drying operation is: the rolled banana flowers and Yunnan large-leaf sun-dried green tea are spread out by 3-5 cm, and a sunny and well-ventilated place is selected to be air-dried until the water content is reduced to less than 10%.

[0016] Furthermore, in the step (2), during the process of the mixture being piled, the temperature in the room is controlled at 30-40°C and the humidity is controlled at 70-85%, and the material is turned over in the room 2-3 times a day.

[0017] Furthermore, in the step (3), during the sealed storage of the mixture, the temperature in the room is controlled at 25-35°C and the humidity is controlled at 70-80%. The height of the mixed material during stacking is 50-70 cm. The room is entered and the materials are turned over once every 2 days. After turning over is completed, the room continues to be sealed.

[0018] Furthermore, in the step (4), the mixture is dried to a moisture content of no more than 14%; the drying is carried out by drying; and the drying temperature does not exceed 60°C.

[0019] Use of the above-mentioned composition in preparing a preparation for inhibiting advanced glycation end products.

[0020] A preparation capable of inhibiting advanced glycation end products comprises the above-mentioned composition.

[0021] Beneficial effects of the present invention:

[0022] The composition containing the oil-tea camellia extract of the present invention comprises the following steps: firstly mixing banana flowers and Yunnan large-leaf sun-dried green tea for piling, so that the banana flowers and the Yunnan large-leaf sun-dried green tea absorb aroma from each other during the mixing process; and in the spreading process, the microorganisms in the Yunnan large-leaf sun-dried green tea are used to achieve preliminary fermentation of active ingredients of the banana flowers, and then the oil-tea camellia extract is added for continued sealed storage, and finally rose flowers are added for aging treatment, so that the inhibitory activity of the composition on advanced glycation end products can be improved, the research and development of products with AGEs inhibitory activity is enriched, a new idea is provided for the research and development of AGEs inhibitors, a new idea is provided for the application of the oil-tea camellia extract, and the research and development of oil-tea related products is enriched. DETAILED DESCRIPTION

[0023] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] Example 1

[0025] A composition containing camellia oleifera extract comprises the following components in parts by weight: one part of camellia oleifera extract, 10 parts of banana flowers, 5 parts of Yunnan large-leaf sun-dried green tea, and 5 parts of rose flowers.

[0026] (1) freshly picked banana flowers and Yunnan large-leaf sun-dried green tea are taken separately, and after removing impurities, washing and drying, the banana flowers are divided (cut or torn) into thin strips with a width of 0.5-1.0 cm, and then withered, killed, rolled, and sun-dried for later use;

[0027] The withering treatment method is as follows: banana flowers and Yunnan large-leaf sun-dried green tea are spread on bamboo mats or bamboo sieves respectively, and placed in a well-ventilated indoor or outdoor shaded place away from direct sunlight to wither until the water content of banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%;

[0028] During the withering process, the temperature is controlled at 180℃; the tea is rolled until the strips are curled and tightly bound, and a little juice oozes out; the sun-drying operation is as follows: spread the rolled banana flowers and Yunnan large-leaf sun-drying green tea by 3-5 cm, and choose a sunny and well-ventilated place to dry them until the moisture content drops below 10%.

[0029] (2) Take sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room for fermentation for 40 days; during the fermentation process, control the temperature in the room at 30° C. and the humidity at 70-85%; enter the room to turn the materials twice a day.

[0030] (3) adding water to the camellia oleifera extract to prepare a suspension, adding the suspension to the mixture and mixing evenly to prepare a mixture, and then drying to a water content of 20%, stacking in a sealed room away from light for storage for 1 day; during the sealed storage of the mixture, the temperature in the room is controlled at 25-35° C., the humidity is controlled at 70-80%, the height of the mixture during the stacking process is 50-70 cm, and the room is entered to turn the mixture every 2 days. After turning the mixture, the room is sealed again;

[0031] (4) After the sealed storage is completed, the mixture is dried by drying until the moisture content is no more than 14% and the drying temperature does not exceed 60° C. Then, rose petals are added, mixed evenly, and aged in a well-ventilated and dry environment for 30 days.

[0032] Example 2

[0033] A composition containing camellia oleifera extract comprises the following components in parts by weight: 3 parts of camellia oleifera extract, 15 parts of banana flowers, 10 parts of Yunnan large-leaf sun-dried green tea, and 8 parts of rose flowers.

[0034] (1) freshly picked banana flowers and Yunnan large-leaf sun-dried green tea are taken separately, and after removing impurities, washing and drying, the banana flowers are divided (cut or torn) into thin strips with a width of 0.5-1.0 cm, and then withered, killed, rolled, and sun-dried for later use;

[0035] The withering treatment method is as follows: banana flowers and Yunnan large-leaf sun-dried green tea are spread on bamboo mats or bamboo sieves respectively, and placed in a well-ventilated indoor or outdoor shaded place away from direct sunlight to wither until the water content of banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%;

[0036] During the withering process, the temperature is controlled at 200℃; the tea leaves are rolled until they are tightly curled and slightly juice oozes out; the sun-drying operation is as follows: spread the rolled banana flowers and Yunnan large-leaf sun-drying green tea leaves by 3-5 cm, and select a sunny and well-ventilated place to dry them until the moisture content drops below 10%.

[0037] (2) Take sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room for fermentation for 50 days; during the fermentation process, control the temperature in the room at 35° C. and the humidity at 70-85%; enter the room to turn the materials three times a day.

[0038] (3) adding water to the camellia oleifera extract to prepare a suspension, adding the suspension to the mixture and mixing evenly to prepare a mixture, and then drying to a water content of 25%, stacking in a sealed room away from light for 2 days; during the sealed storage of the mixture, the temperature in the room is controlled at 25-35° C., the humidity is controlled at 70-80%, and the height of the mixture during stacking is 50-70 cm. The room is entered to turn the mixture every 2 days. After turning the mixture, the room is sealed again;

[0039] (4) After the sealed storage is completed, the mixture is dried by drying until the moisture content is no more than 14% and the drying temperature does not exceed 60° C. Then, rose petals are added, mixed evenly, and aged in a well-ventilated and dry environment for 40 days.

[0040] Example 3

[0041] A composition containing camellia oleifera extract, comprising the following components in weight: 5 parts of camellia oleifera extract, 20 parts of banana flowers, 15 parts of Yunnan large-leaf sun-dried green tea, and 10 parts of rose flowers. (1) Freshly picked banana flowers and Yunnan large-leaf sun-dried green tea are taken separately, and after removing impurities, washing and drying, the banana flowers are divided (cut or torn) into thin strips with a width of 0.5-1.0 cm, and then withered, killed, rolled, and sun-dried for later use;

[0042] The withering treatment method is as follows: banana flowers and Yunnan large-leaf sun-dried green tea are spread on bamboo mats or bamboo sieves respectively, and placed in a well-ventilated indoor or outdoor shaded place away from direct sunlight to wither until the water content of banana flowers and Yunnan large-leaf sun-dried green tea reaches 60-70%;

[0043] During the withering process, the temperature is controlled at 220℃; the tea is rolled until the strips are curled and tightly together, and a little juice oozes out; the sun-drying operation is as follows: spread the rolled banana flowers and Yunnan large-leaf sun-drying green tea by 3-5 cm, and choose a sunny and well-ventilated place to dry them until the moisture content drops below 10%.

[0044] (2) Take sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mix them evenly to obtain a mixture; place the mixture in a room for fermentation for 60 days; during the fermentation process, control the temperature in the room at 40° C. and the humidity at 70-85%; enter the room to turn the materials three times a day.

[0045] (3) adding water to the camellia oleifera extract to form a suspension, adding the suspension to the mixture and mixing evenly to form a mixture, and then drying to a water content of 30%, stacking in a sealed room away from light for storage for 3 days; during the sealed storage of the mixture, the temperature in the room is controlled at 25-35° C., the humidity is controlled at 70-80%, and the height of the mixture during the stacking process is 50-70 cm. The room is entered to turn the mixture every 2 days. After turning the mixture, the room is sealed again;

[0046] (4) After the sealed storage is completed, the mixture is dried by drying until the moisture content is no more than 14% and the drying temperature does not exceed 60° C. Then, rose petals are added, mixed evenly, and aged in a well-ventilated and dry environment for 60 days.

[0047] In Examples 1-3, the banana flowers are freshly picked banana flowers, and the rose flowers are dried rose petals.

[0048] The method for preparing the camellia oleifera extract comprises the following steps:

[0049] The camellia seed cake is degreased by adding petroleum ether in a solid-liquid ratio of 1:8; the filtrate is removed by filtration, and the solid part is dried in an electric heating forced air drying oven at 50-60°C to obtain the defatted cake, and then 5-6 times the mass of the defatted cake is added with water for heating and boiling for 20-30 minutes, and the insoluble matter is removed to obtain the filtrate, and the filtrate is pre-cooled and vacuum freeze-dried to obtain the product. The petroleum ether used must comply with the "National Food Safety Standard Food Additive Petroleum Ether" (GB 1886.59-2015).

[0050] The composition prepared in the above examples has the activity of inhibiting advanced glycation end products, can be used to prepare a preparation for inhibiting advanced glycation end products, and is particularly suitable for the preparation of tea bags.

[0051] The inhibitory activity of the composition of the present invention on AGEs

[0052] The classic protein glycosylation model (BSA-FRU) was selected for activity determination. The reaction system contained 60 mg / mL BSA, 1.5 M FRU, which was prepared in 50 mM PBS (containing 0.02% NaN3, pH 7.4). Each sample was added at a dose of 3.0 mg / mL (calculated based on the amount of the prepared composition). Each sample was brewed with 6 times boiling water, and the filtrate was concentrated to the same mass as the sample and then added. The test tube was cultured in an incubator at 37°C (SPX-250B-Z, Boxun, Shanghai, China) for 7 days. The fluorescence intensity was measured at an excitation / emission wavelength of 325 / 440 nm by a fluorescence spectrophotometer (Hitachi F-7000, Tokyo, Japan), the differences in AGEs were compared, and the inhibition rate of each fraction was calculated.

[0053] Inhibition rate (%) = (λ 对照 -λ 样品 ) / λ 对照 ×100%

[0054] Among them, λ 对照 is the fluorescence intensity of the control group after culture, λ 对照 is the fluorescence intensity of the control group after culture.

[0055] The preparation methods of the samples to be tested are shown in Table 1.

[0056] Sample 1: A composition was prepared according to the proportions and preparation method of Example 1.

[0057] Sample 2: A composition was prepared according to the proportions and preparation method of Example 2.

[0058] Sample 3: A composition was prepared according to the ratio and preparation method of Implementation 3.

[0059] Sample 4: Referring to the material proportion and preparation method of Example 2, without adding banana flowers. Sample 5: Referring to the material proportion and preparation method of Example 2, without adding Yunnan large-leaf sun-dried green tea.

[0060] Sample 6: Referring to the material ratio and preparation method of Example 2, no camellia oleifera extract is added in step (3), and the other operations remain unchanged.

[0061] Sample 7: Refer to the material ratio of Example 2, but in the preparation process, banana flowers, Yunnan large-leaf sun-dried green tea and camellia oleifera extract in step (2) are added all at once, dried to a moisture content of 10%, and then composted for 52 days, and then processed according to the method of step (4).

[0062] The AGEs inhibition rate of each sample is shown in Table 1.

[0063] Table 1 Comparison of AGEs inhibition rates of different samples

[0064]

[0065]

[0066] As can be seen from Table 1, the above 7 samples to be tested all have certain AGEs inhibitory activity. Among them, sample 1-3 has the strongest inhibitory activity. The material ratio of samples 4-6 was changed. Although the preparation method is the same as that of Example 2, the AGEs inhibitory activity is still reduced to varying degrees relative to samples 1-3. Although sample 7 uses the same material ratio as Example 2, all materials are sealed and fermented at one time, and the AGEs inhibition rate is improved relative to sample 5, but it is lower than samples 4 and 6, and significantly lower than samples 1-3. The above experimental results show that the treatment method of the present invention, which first seals and ferments banana flowers, Yunnan large-leaf sun-dried green tea and rose flowers, and then adds tea oil extract to continue fermentation, can significantly improve the AGEs of the prepared composition. Whether it is changing the composition of the materials or changing the fermentation method, it will significantly affect the AGEs inhibitory activity.

[0067] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composition containing a camellia oleifera extract, characterized in that: The invention comprises the following components in parts by weight: 1-5 parts of camellia oleifera extract, 10-20 parts of banana flowers, 5-15 parts of Yunnan large-leaf sun-dried green tea and 5-10 parts of rose flowers.

2. The composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 3 parts of camellia oleifera extract, 15 parts of banana flowers, 10 parts of Yunnan large-leaf sun-dried green tea and 8 parts of rose flowers.

3. The composition according to claim 1, characterized in that The banana flowers are freshly picked banana flowers, and the rose flowers are dried rose flowers.

4. The composition according to claim 1, characterized in that The method for preparing the camellia oleifera extract comprises the following steps: The tea seed cake is defatted and dried to obtain defatted cake, and then water 5-6 times the mass of the defatted cake is added and heated and boiled for 20-30 minutes, insoluble matter is removed to obtain a filtrate, and the filtrate is dried to obtain the product.

5. A method for preparing a composition containing a camellia oleifera extract according to any one of claims 1 to 4, characterized in that: The following steps are involved: (1) freshly picked banana flowers and Yunnan large-leaf sun-dried green tea are taken separately, and after removing impurities, washing and drying, the banana flowers are cut into thin strips, and then withered, fixed, rolled, and sun-dried for use; (2) taking sun-dried banana flowers and Yunnan large-leaf sun-dried green tea, mixing them evenly to obtain a mixture; and placing the mixture in a room for 40-60 days; (3) adding water to the camellia oleifera extract to prepare a suspension, adding the suspension to the mixture and mixing evenly to prepare a mixture, then drying to a water content of 20-30%, and stacking in a sealed room away from light for 1-3 days; (4) After the sealed storage is completed, dry it; then add dried rose petals, mix well, and age it for at least 30 days.

6. The preparation method according to claim 5, characterized in that: In the step (1), the banana flowers are cut or torn into thin strips with a width of 0.5-1.0 cm; the banana flowers and Yunnan large-leaf sun-dried green tea are spread on bamboo mats or bamboo sieves respectively, and placed in a well-ventilated indoor or outdoor shaded place away from direct sunlight to wither until the water content of the banana flowers and the Yunnan large-leaf sun-dried green tea reaches 60-70%; the temperature is controlled at 180-220° C. during the withering process; the banana flowers are rolled until the strips are curled and tightly bound and a little juice oozes out; the sun-drying operation is: the rolled banana flowers and Yunnan large-leaf sun-dried green tea are spread out by 3-5 cm, and a sunny and well-ventilated place is selected to be air-dried until the water content is reduced to less than 10%.

7. The preparation method according to claim 5, characterized in that: In the step (2), during the process of the mixture being piled, the temperature in the room is controlled at 30-40°C and the humidity is controlled at 70-85%; the room is entered 2-3 times a day to turn the mixture; in the step (3), during the process of the mixture being sealed and stored, the temperature in the room is controlled at 30-40°C and the humidity is controlled at 70-85%, the height of the mixed material during the stacking process is 50-70 cm, the room is entered once every 2 days to turn the mixture, and after turning the mixture, the room continues to be sealed.

8. The preparation method according to claim 5, characterized in that: In the step (4), the mixture is dried to a moisture content of no more than 14%; the drying is carried out by drying; and the drying temperature does not exceed 60°C.

9. Use of the composition according to any one of claims 1 to 4 in the preparation of a preparation for inhibiting advanced glycation end products.

10. A preparation capable of inhibiting advanced glycation end products, comprising the composition according to any one of claims 1 to 4.

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