Method for preparing goose gout powder by environment-friendly enzymatic fractional concentration technology

Abstract

The invention discloses a method for preparing goose gout powder by an environment-friendly enzymatic fractional concentration technology, and relates to the technical field of preparation of goose gout agents.The method comprises the following steps that 1, cinnabar, rhizoma alismatis, polygonum aviculare, plantain herb, lalang grass rhizome, poria cocos, akebiaquinata and coix seeds are prepared and evenly mixed together; step 2, drying the mixture obtained in the step 1; step 3, carrying out grinding treatment on the dried mixture, wherein the fineness range of the ground mixture is 20-40 meshes; the raw materials are subjected to instantaneous wall-breaking treatment and subjected to three-stage concentration by adopting various microbial enzyme preparations, effective substances such as triterpenoids, saponin, flavone and the like for treating goose gout can be separated from cellulose and hemicellulose through instantaneous wall-breaking treatment, and then the effective substances are subjected to enzymatic three-stage concentration, so that the goose gout can be effectively treated. Cellulose, hemicellulose and protein substances are effectively separated, so that the extraction rate of effective substances such as triterpenoids, saponins and flavones for treating goose gout is effectively increased.

Description

Technical Field

[0001] The invention relates to the technical field of goose gout medicament preparation, in particular to a method for preparing goose gout powder using an environmentally friendly enzymatic graded concentration technology. Background Art

[0002] The existing treatment methods for gout in geese are to simply steam and cook the Chinese herbal medicines, and then feed the geese with the steamed liquid to treat gout. The existing methods have the following shortcomings: 1. Due to the inherent characteristics of Chinese herbal medicine, the effective substances for treating gout such as triterpenes, saponins and flavonoids are wrapped and combined with cellulose, hemicellulose, etc., so it is difficult to separate the effective substances for treating gout such as triterpenes, saponins and flavonoids from the wrapping and combination of cellulose and hemicellulose by using only simple steaming treatment. This directly leads to the difficulty of obtaining high-concentration effective substances for treating gout such as triterpenes, saponins and flavonoids by simply steaming Chinese herbal medicine; 2. The existing simple steaming process requires a long time of steaming, which will seriously destroy the effective substances in Chinese herbal medicine for treating gout; 3. The medicinal residues produced by the existing simple steaming treatment method contain more than 80% moisture, which is easy to produce a foul odor and cause environmental pollution.

[0003] In view of the above problems, we provide a method for preparing goose gout powder using an environmentally friendly enzymatic graded concentration technology to solve the above-mentioned problems. Summary of the invention

[0004] The purpose of the present invention is to provide a method for preparing goose gout powder by using an environmentally friendly enzymatic graded concentration technology to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: A method for preparing goose gout powder by environmentally friendly enzymatic graded concentration technology comprises the following steps: Step 1: prepare rhizoma rubiae, rhizoma alismatis, rhizoma scutellariae, herba plantaine, rhizoma imperatae, rhizoma tuckahoe, rhizoma akebiae and coix seeds, and mix the above raw materials evenly; Step 2, drying the mixture obtained in step 1; Step 3, grinding the dried mixture to a fineness range of 20-40 mesh; Step 4, loading the ground raw material mixture into an instantaneous wall breaking bin for instantaneous wall breaking treatment; Step 5, taking out the raw material mixture after instantaneous cell wall breaking treatment, loading the mixture into an enzymatic graded concentration reactor, performing primary concentration, separating the cellulose substances in the raw material mixture, conveying the reaction liquid after primary concentration to a centrifugal device for solid-liquid separation, and conveying the supernatant to a by-product tank; Step 6, transporting the precipitate treated in step 5 back to the enzymatic graded concentration reactor for secondary concentration to separate the hemicellulose substances in the raw material mixture, transporting the reaction liquid after the secondary concentration to a centrifugal device for solid-liquid separation, and transporting the supernatant to a by-product tank; Step 7, transporting the precipitate treated in step 6 back to the enzyme graded concentration reactor for three-stage concentration to separate the protein substances in the raw material mixture, transporting the reaction liquid after the three-stage concentration to a centrifugal device for solid-liquid separation, and transporting the supernatant to a by-product tank; Step 8: Dry the precipitate treated in step 7 at 65-75 degrees Celsius for 5-6 hours, control the moisture content to 10-12%, and crush it to 100-120 mesh to obtain goose gout concentrated powder. The supernatant treated in steps 5, 6 and 7 can be used as a raw material for organic foliar fertilizer.

[0006] As a further scheme of the present invention: the amounts of the raw materials in step 1 are: 1-2 parts of Rhizoma Chuanxiong, 2-3 parts of Rhizoma Zedoariae, 1-2 parts of Rhizoma Polygoni Multiflori, 2-4 parts of Herba Plantaginis, 5-8 parts of Rhizoma Imperatae, 1-2 parts of Rhizoma Poriae, 1-2 parts of Rhizoma Akebiae, and 2-4 parts of Rhizoma Coix.

[0007] As a further solution of the present invention: in the drying process of step 2, the drying temperature is 80-90 degrees Celsius, the drying time is 4-6 hours, and the moisture content of the mixture after drying is 12%-15%.

[0008] As a further solution of the present invention: the condition for instantaneous wall breaking in step 4 is that after the raw material mixture is charged, the temperature is quickly raised to 120-150 degrees Celsius, the pressure is controlled at 0.25-0.5 MPa, and after maintaining the pressure for 15-45 seconds, the pressure is quickly released to rapidly reduce the temperature and pressure.

[0009] As a further scheme of the present invention: the specific operation of the primary concentration is to cool the raw material mixture to 55-65 degrees Celsius, add water in a ratio of 1:1-3 according to the volume of the raw material mixture, mix and stir for 120-240 seconds, add cellulase in a mass ratio of 0.25%-0.45%, and keep warm at 35-45 degrees Celsius for 30-60 minutes.

[0010] As a further solution of the present invention: the rotation speed of the centrifugal device in step 5 is 2500-4500 rpm, and the centrifugation time is 15-20 minutes.

[0011] As a further solution of the present invention: the specific operation of the secondary concentration is: according to the volume of the precipitate, water is added in a ratio of 1:1-1.5, and mixed and stirred for 150-210 seconds, and hemicellulase is added in a mass ratio of 0.35%-0.5% and kept warm at 40-45 degrees Celsius for 60-90 minutes.

[0012] As a further solution of the present invention: the rotation speed of the centrifugal device in step 6 is 3500-4500 rpm, and the centrifugation time is 20-30 minutes.

[0013] As a further solution of the present invention: the specific operation of the three-stage concentration is: according to the volume of the precipitate, water is added in a ratio of 1:1.25-1.75, and then mixed and stirred for 150-240 seconds, and protein enzyme is added at a mass ratio of 0.1-0.2%, and then kept warm at 40-45 degrees Celsius for 60-90 minutes.

[0014] As a further solution of the present invention: the rotation speed of the centrifugal device in step 7 is 4000-5000 rpm, and the centrifugation time is 10-15 minutes.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention performs instantaneous wall-breaking treatment on the raw materials and adopts a variety of microbial enzyme preparations for three-stage concentration. Through the instantaneous wall-breaking treatment, effective substances for treating gout such as triterpenes, saponins and flavonoids can be separated from cellulose and hemicellulose. Then, through the three-stage enzymatic concentration technology, cellulose, hemicellulose and protein substances can be effectively separated, thereby effectively improving the extraction rate of effective substances for treating gout such as triterpenes, saponins and flavonoids.

[0016] 2. The present invention adopts enzyme catalysis technology and utilizes the specific characteristics of enzyme catalysts, and does not produce any destructive effect on these effective substances.

[0017] 3. The by-products produced by the present invention can be used as raw materials for organic foliar fertilizer without causing any pollution to the environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the preparation process of the present invention. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] See also Figure 1 In an embodiment of the present invention, a method for preparing goose gout powder by an environmentally friendly enzymatic graded concentration technology comprises the following steps: Step 1, prepare Zhuling, Zexie, Polygonum multiflorum, Plantago, Imperata root, Poria, Akebia and Coix seeds, the amount of each raw material is 2 parts of Zhuling, 2 parts of Zexie, 1 part of Polygonum multiflorum, 4 parts of Plantago, 8 parts of Imperata root, 2 parts of Poria, 2 parts of Akebia and 4 parts of Coix seeds, and mix the above raw materials evenly together; Step 2, drying the mixture obtained in step 1 at a drying temperature of 85 degrees Celsius for 5 hours, and the moisture content of the dried mixture is 12%; Step 3, grinding the dried mixture to a fineness of 40 mesh; Step 4: Load the ground raw material mixture into the instantaneous wall breaking bin for instantaneous wall breaking treatment. After the raw material mixture is loaded, the temperature is quickly raised to 150 degrees Celsius, the pressure is controlled at 0.5 MPa, and after maintaining the pressure for 30 seconds, the pressure is quickly released to rapidly reduce the temperature and pressure; Step 5, taking out the raw material mixture that has undergone instantaneous wall breaking treatment, and loading the mixture into an enzymatic graded concentration reactor for primary concentration. The specific operation of the primary concentration is to cool the raw material mixture to 60 degrees Celsius, add water in a ratio of 1:2 according to the volume of the raw material mixture, mix and stir for 180 seconds, add cellulase at a mass ratio of 0.25%, and then keep warm at 45 degrees Celsius for 60 minutes. The cellulose substances in the raw material mixture are separated by primary concentration, and the reaction liquid after primary concentration is transported to a centrifugal device for solid-liquid separation. The speed of the centrifugal device is 4500 revolutions per minute, and the centrifugal time is 15 minutes. Then, the supernatant is transported to a by-product tank; Step 6, transporting the precipitate treated in step 5 back to the enzyme graded concentration reactor for secondary concentration, the specific operation of the secondary concentration is: adding water in a ratio of 1:1.5 according to the volume of the precipitate and mixing and stirring for 150 seconds, adding hemicellulase at a mass ratio of 0.35% and keeping warm at 42 degrees Celsius for 75 minutes, separating the hemicellulose substances in the raw material mixture through secondary concentration, transporting the reaction liquid after the secondary concentration to a centrifugal device for solid-liquid separation, the speed of the centrifugal device is 4500 rpm, the centrifugal time is 20 minutes, and then transporting the supernatant to the by-product tank; Step 7, transporting the precipitate treated in step 6 back to the enzyme graded concentration reactor for three-stage concentration, and the specific operation of the three-stage concentration is: adding water in a ratio of 1:1.75 according to the volume of the precipitate, mixing and stirring for 210 seconds, adding protein enzyme at a mass ratio of 0.15%, and keeping warm at 42 degrees Celsius for 60 minutes; separating the protein substances in the raw material mixture through three-stage concentration, transporting the reaction liquid after the three-stage concentration to a centrifugal device for solid-liquid separation, the speed of the centrifugal device is 5000 revolutions per minute, the centrifugal time is 15 minutes, and then transporting the supernatant to the by-product tank; Step 8, drying the precipitate treated in step 7 at 75 degrees Celsius for 5 hours, controlling the moisture content to 12%, and crushing to 120 mesh to obtain goose gout concentrated powder; The supernatant after being processed in steps 5, 6 and 7 can be used as a raw material for organic foliar fertilizer.

[0021] The present invention performs instantaneous wall-breaking treatment on the raw materials and uses a variety of microbial enzyme preparations for three-stage concentration. Through the instantaneous wall-breaking treatment, effective substances for treating gout such as triterpenes, saponins and flavonoids can be separated from cellulose and hemicellulose. Then, through the three-stage enzymatic concentration technology, cellulose, hemicellulose and protein substances can be effectively separated, thereby effectively improving the extraction rate of effective substances for treating gout such as triterpenes, saponins and flavonoids.

[0022] It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or basic features of the present invention. Although this specification is described in accordance with the implementation modes, not every implementation mode includes only one technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A method for preparing goose gout powder by environmentally friendly enzymatic graded concentration technology, characterized in that; The following steps are involved: Step 1: prepare rhizoma rubiae, rhizoma alismatis, rhizoma scutellariae, herba plantaine, rhizoma imperatae, rhizoma tuckahoe, rhizoma akebiae and coix seeds, and mix the above raw materials evenly; Step 2, drying the mixture obtained in step 1; Step 3, grinding the dried mixture to a fineness range of 20-40 mesh; Step 4, loading the ground raw material mixture into an instantaneous wall breaking bin for instantaneous wall breaking treatment; Step 5, taking out the raw material mixture after instantaneous cell wall breaking treatment, loading the mixture into an enzymatic graded concentration reactor, performing primary concentration, separating the cellulose substances in the raw material mixture, conveying the reaction liquid after primary concentration to a centrifugal device for solid-liquid separation, and conveying the supernatant to a by-product tank; Step 6, transporting the precipitate treated in step 5 back to the enzyme graded concentration reactor for secondary concentration to separate the hemicellulose substances in the raw material mixture, transporting the reaction liquid after the secondary concentration to a centrifugal device for solid-liquid separation, and transporting the supernatant to a by-product tank; Step 7, transporting the precipitate treated in step 6 back to the enzyme graded concentration reactor for three-stage concentration to separate the protein substances in the raw material mixture, transporting the reaction liquid after the three-stage concentration to a centrifugal device for solid-liquid separation, and transporting the supernatant to a by-product tank; Step 8, drying the precipitate treated in step 7 at 65-75 degrees Celsius for 5-6 hours, controlling the moisture content to 10-12%, and crushing to 100-120 mesh to obtain goose gout concentrated powder; The supernatant after being processed in steps 5, 6 and 7 can be used as a raw material for organic foliar fertilizer.

2. The method for preparing gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The amounts of the raw materials in step 1 are: 1-2 parts of Rhizoma Chuanxiong, 2-3 parts of Rhizoma Zedoariae, 1-2 parts of Rhizoma Polygoni Multiflori, 2-4 parts of Herba Plantaginis, 5-8 parts of Rhizoma Imperatae, 1-2 parts of Rhizoma Poriae, 1-2 parts of Rhizoma Akebiae, and 2-4 parts of Rhizoma Coix.

3. The method for preparing gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: In the drying process of step 2, the drying temperature is 80-90 degrees Celsius, the drying time is 4-6 hours, and the moisture content of the mixture after drying is 12%-15%.

4. The method for preparing gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The conditions for instantaneous wall breaking in step 4 are: after the raw material mixture is charged, the temperature is quickly raised to 120-150 degrees Celsius, the pressure is controlled at 0.25-0.5 MPa, and after maintaining the pressure for 15-45 seconds, the pressure is quickly released to rapidly reduce the temperature and pressure.

5. The method for preparing goose gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The specific operation of the primary concentration is to cool the raw material mixture to 55-65 degrees Celsius, add water in a ratio of 1:1-3 according to the volume of the raw material mixture, mix and stir for 120-240 seconds, add cellulase in a mass ratio of 0.25%-0.45%, and keep warm at 35-45 degrees Celsius for 30-60 minutes.

6. The method for preparing gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The rotation speed of the centrifugal device in step 5 is 2500-4500 rpm, and the centrifugation time is 15-20 minutes.

7. The method for preparing gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The specific operation of the secondary concentration is: add water in a ratio of 1:1-1.5 according to the volume of the precipitate, mix and stir for 150-210 seconds, add hemicellulase in a mass ratio of 0.35%-0.5%, and keep warm at 40-45 degrees Celsius for 60-90 minutes.

8. The method for preparing gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The rotation speed of the centrifugal device in step 6 is 3500-4500 rpm, and the centrifugation time is 20-30 minutes.

9. The method for preparing gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The specific operation of the three-stage concentration is: add water in a ratio of 1:1.25-1.75 according to the volume of the precipitate, mix and stir for 150-240 seconds, add protein enzyme in a mass ratio of 0.1-0.2%, and keep warm at 40-45 degrees Celsius for 60-90 minutes.

10. The method for preparing goose gout powder by an environmentally friendly enzymatic graded concentration technology according to claim 1, characterized in that: The rotation speed of the centrifugal device in step 7 is 4000-5000 rpm, and the centrifugation time is 10-15 minutes.

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