Composition for expelling cold and dampness and slimming body and application thereof

Abstract

The invention relates to the technical field of biological preparations for expelling cold-dampness and slimming, in particular to a composition for expelling cold-dampness and slimming and application thereof. The invention provides a composition for expelling cold-dampness and slimming. The composition is prepared from the following components: a lotus leaf extract, a corn poppy extract, an anthemis nobilis extract, a poria cocos extract, a ginger root extract, a cinnamon extract, a pogostemon cablin extract, a moringa seed extract, a crocus sativus extract, a wormwood extract and functional polypeptide. By using the composition and the cream, the problems of abdominal obesity, edema and the like of people with cold-dampness can be effectively improved; meanwhile, the fat content in the body can be effectively reduced, and the area of fat cells is reduced; the levels of leptin and triglyceride are improved, and the health of blood fat is maintained; and the endokephin can be increased, so that the positive emotion maintenance can be maintained. Through the multiple effects, the sub-health problem of people with cold-dampness constitution is comprehensively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological preparations for expelling cold and dampness and slimming the body, and in particular to a composition for expelling cold and dampness and slimming the body and application thereof. Background Art

[0002] Cold and dampness is a common disease in traditional Chinese medicine. In traditional Chinese medicine theory, cold and dampness constitution can be divided into two types: exogenous cold and dampness and endogenous cold and dampness. Exogenous cold and dampness is caused by the invasion of cold and damp evil spirits from the outside world, while endogenous cold and dampness is caused by damaged spleen yang or spleen and kidney yang deficiency, which leads to the retention of dampness in the body. This physical state not only causes symptoms such as chills, cold limbs, and joint pain, but may also affect metabolism, leading to endocrine disorders, and then inducing obesity and other health problems. From the perspective of modern medicine, cold and dampness constitution may be related to physiological mechanisms such as poor blood circulation, obstructed lymphatic return, excessive strain, insufficient energy supply, and decreased immune system function. These mechanisms will also affect the body's metabolic balance, promote fat accumulation, and increase the risk of disease.

[0003] For people with cold and damp constitution, local edema, especially lower limb edema, is a common symptom. This is because the dampness in the body is too heavy, which affects the normal metabolism of water in the human body, causing water to be retained in the body, especially in the lower limbs, forming edema. In addition, cold and damp constitution may also increase the risk of cellulitis, which is a widespread, diffuse, suppurative inflammation of the skin and subcutaneous tissue, mostly caused by hemolytic streptococci, manifested as local redness, swelling, heat, and pain in the skin, and quickly spreads to the surrounding areas.

[0004] In addition, people with cold and damp constitutions often face greater challenges in body management. On the one hand, due to insufficient energy supply and poor absorption of nutrients, metabolism slows down, which can easily cause fat to accumulate in the body, especially in the waist, abdomen, and thighs, making weight loss more difficult. On the other hand, local edema caused by dampness, such as lower limb edema, further exacerbates the unsightly appearance of the body. In addition, cold and dampness may also affect the spleen and stomach function, leading to lack of appetite or overeating, further exacerbating the difficulty of weight control.

[0005] Traditional Chinese medicine methods, such as Chinese medicine treatment, moxibustion, and cupping, have certain effects in regulating cold and damp constitution, but there are problems such as large individual differences, complex operations, and inconsistent long-term effects. Modern medicine may deal with health problems caused by cold and damp constitution by improving blood circulation, promoting lymphatic return, and regulating immune function, but there are also limitations such as drug side effects, oral first-pass effect, and long treatment cycle.

[0006] There are many kinds of products for expelling cold and dampness in the current market, including expelling dampness drinks, expelling dampness agents, traditional Chinese medicine instruments and medicines, etc. However, these products have limitations to varying degrees in terms of effect, convenience, safety and cost, and it is difficult to meet the needs of people with cold and damp constitution for comprehensive, efficient and safe expelling cold and dampness and body management.

[0007] Therefore, it is of great significance to develop a product that combines the theories of traditional Chinese and Western medicine to be more efficient, safe, convenient and economical in removing cold and dampness and slimming the body, while helping people with cold and dampness constitution to manage their body more effectively, improve their health status and enhance their quality of life. Summary of the invention

[0008] The purpose of the present invention is to provide a composition for removing cold and dampness and slimming the body and its application. The cream prepared by using the composition and base cream can effectively remove cold and dampness, slim the body and improve human sub-health.

[0009] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0010] The invention provides a composition for expelling dampness and slimming the body, comprising the following components in parts by weight: 0.1 to 3 parts of lotus leaf extract, 0.1 to 2 parts of corn poppy flower extract, 0.01 to 0.1 parts of white chamomile flower extract, 0.1 to 3 parts of Poria cocos extract, 0.1 to 3 parts of ginger root extract, 0.1 to 3 parts of cinnamon extract, 0.1 to 0.2 parts of patchouli extract, 0.01 to 1 parts of moringa seed extract, 0.01 to 0.5 parts of saffron extract, 0.01 to 0.5 parts of wormwood extract, and 0.1 to 1 parts of functional polypeptide.

[0011] Preferably, the functional polypeptide includes one or more of collagen amino acids, oligopeptides, and heptapeptides.

[0012] The present invention also provides application of the composition in preparing a cold-damp expelling and body slimming preparation.

[0013] The present invention also provides a cold-damp-dispelling and body-slimming cream, comprising the following components in parts by weight: 65-101 parts of an aqueous phase, 7-24 parts of an oil phase, 3-12 parts of an emulsifier, 0.01-0.2 parts of a penetration enhancer, and 0.4-16 parts of a cold-damp-dispelling and body-slimming composition;

[0014] The composition for expelling cold and dampness and slimming the body is the composition described.

[0015] Preferably, the aqueous phase comprises one or more of water, glycerin, butylene glycol, propylene glycol, xanthan gum, carbomer, and chlorphenesin;

[0016] The oil phase comprises one or more of hydrogenated polyisobutene, rice bran oil, stearyl glycyrrhetinate, isononyl isononanoate, polydimethylsiloxane, and phenoxyethanol;

[0017] The emulsifier includes one or more of cetearyl alcohol, isoceteth-20, glyceryl stearate, and methyl gluceth-20;

[0018] The penetration enhancer includes pepper extract.

[0019] The present invention also provides a method for preparing the cream, comprising the following steps:

[0020] (1) mixing an oil phase and an emulsifier, and heating to obtain a mixture A;

[0021] (2) mixing the mixture A and water, and homogenizing to obtain a mixture B;

[0022] (3) mixing mixture B and a penetration enhancer to obtain a base cream;

[0023] (4) After cooling the base cream to below 45°C, the base cream is mixed with the cold-dampness-removing and body-slimming composition to obtain a cream.

[0024] Preferably, in step (1), the heating temperature is 70-90°C.

[0025] Preferably, in step (2), the mixing is stirring mixing, and the stirring speed is 5 to 45 rpm;

[0026] The rotation speed of the homogenizer is 2000-3000 rpm; the homogenization time is 2-4 min; and the vacuum degree of the homogenizer is -0.7-0.5 bar.

[0027] Preferably, in step (3), the mixing is stirring mixing, and the stirring speed is 5 to 35 rpm; the vacuum degree of the mixing is -0.7 to -0.5 bar.

[0028] Preferably, in step (4), the mixing is stirring mixing, and the stirring speed is 5 to 35 rpm; the vacuum degree of the mixing is -0.7 to -0.5 bar.

[0029] Beneficial effects of the present invention:

[0030] The invention provides a composition for expelling dampness and slimming the body, wherein lotus leaf extract, corn poppy seed extract and white chamomile flower oil can promote fat burning; Poria cocos extract and patchouli extract can strengthen the spleen and replenish qi, thereby enhancing the spleen's transportation and transformation function, improving the qi and blood circulation and metabolic environment in the body, timely expelling metabolic waste liquid from the body, avoiding the growth of dampness, and enhancing the body's resistance to external evils such as dampness and cold; ginger root extract, cinnamon extract, moringa seed extract, saffron extract and wormwood extract can promote blood circulation and promote the expulsion of dampness and cold in the body; functional polypeptides, with collagen amino acids, oligopeptides, heptapeptides and the like as active ingredients, such substances can act as chemical messengers and participate in regulating life processes such as cell growth, development, energy supply and metabolism, and these adjustments can help improve the microcirculation in the body, promote the circulation of qi and blood, and help the rapid realization of the effect of expelling dampness. By rationally matching the above composition, the cream prepared by using the composition and the matrix cream has the effects of promoting fat burning, improving transportation and transformation capacity, promoting blood microcirculation, improving metabolism, etc., and can effectively expel cold and dampness, thereby achieving the effect of slimming and improving sub-health of the body.

[0031] The cold-damp-dispelling and slimming cream of the present invention innovatively combines functional polypeptides with traditional Chinese medicine extracts through scientific and reasonable compatibility. The cream improves the transportation and transformation of nutrients by strengthening the spleen, strengthens the metabolism and absorption of substances; improves cell vitality and microcirculation in the body, promotes the circulation of qi and blood, strengthens the transportation of substances, realizes the good circulation of "water vapor" in the human body, and creates a healthy collective environment; at the same time, it targets fat cells and promotes the burning and decomposition of fat cells. Through the joint action of multiple pathways, the ultimate goal of cold-damp-dispelling, slimming and improving human sub-health is achieved. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The results of the mouse weight and fat test, where a is the weight change chart of the mouse from 1 to 15 weeks, b is the weight comparison chart of the mouse at 7 weeks and 15 weeks, c is the statistical chart of the mouse fat mass, and d is the statistical chart of the mouse body fat ratio;

[0033] Figure 2 Figures are fat cell images of mice, where a is a fat cell image of mice in the blank group, b is a fat cell image of mice in the control group, c is a fat cell image of mice in the matrix cream group, d is a fat cell image of mice in the cold-dampness-removing slimming cream group, and e is a statistical image of the fat cell area of ​​mice;

[0034] Figure 3 This is a statistical chart of leptin content in mouse blood;

[0035] Figure 4 This is a statistical chart of triglyceride content in mice;

[0036] Figure 5 This is a statistical chart of β-endorphin content in mice;

[0037] Figure 6 This is a comparison before and after using the cream described in the embodiment in Experimental Example 2, wherein the left side is after using the cream, and the right side is before using the cream. DETAILED DESCRIPTION

[0038] The present invention provides a composition for expelling cold and dampness and slimming the body, comprising the following components in parts by weight:

[0039] 0.1 to 3 parts of lotus leaf extract, preferably 1.55 parts;

[0040] 0.1-2 parts of corn poppy flower extract, preferably 1.05 parts;

[0041] 0.01-0.1 parts of Anthemis nobilis flower extract, preferably 0.055 parts;

[0042] 0.1 to 3 parts of Poria cocos extract, preferably 1.55 parts;

[0043] 0.1 to 3 parts of ginger root extract, preferably 1.55 parts;

[0044] 0.1 to 3 parts of cinnamon extract, preferably 1.55 parts;

[0045] Patchouli extract 0.1-0.2 parts, preferably 0.15 parts

[0046] 0.01 to 1 part of Moringa seed extract, preferably 0.055 part;

[0047] 0.01-0.5 parts of saffron extract, preferably 0.255 parts;

[0048] 0.01-0.5 parts of mugwort extract, preferably 0.255 parts

[0049] 0.1 to 1 part of the functional polypeptide, preferably 0.55 part.

[0050] In the present invention, the functional polypeptide includes one or more of collagen amino acids, oligopeptides, and heptapeptides;

[0051] When the functional polypeptide is collagen amino acid, oligopeptide and heptapeptide, the mass ratio of collagen amino acid, oligopeptide and heptapeptide is 1:3-7:1-2, preferably 1:5:1.5.

[0052] The present invention also provides application of the composition in preparing a cold-damp expelling and body slimming preparation.

[0053] The present invention also provides a cream for removing cold and dampness and slimming the body, comprising the following components in parts by weight:

[0054] 65 to 101 parts of aqueous phase, preferably 75 to 91 parts, more preferably 83 parts;

[0055] 7 to 24 parts of the oil phase, preferably 12 to 19 parts, more preferably 15.5 parts;

[0056] 3 to 12 parts of emulsifier, preferably 6 to 9 parts, more preferably 7.5 parts;

[0057] 0.01 to 0.2 parts of a penetration enhancer, preferably 0.06 to 0.14 parts, more preferably 0.105 parts;

[0058] 0.4 to 16 parts of the composition for expelling cold and dampness and slimming the body, preferably 6.4 to 10 parts, more preferably 8.2 parts;

[0059] The composition for expelling cold and dampness and slimming the body is the composition described.

[0060] In the present invention, the aqueous phase includes one or more of water, glycerin, butylene glycol, propylene glycol, xanthan gum, carbomer, and chlorphenesin;

[0061] The oil phase comprises one or more of hydrogenated polyisobutene, rice bran oil, stearyl glycyrrhetinate, isononyl isononanoate, polydimethylsiloxane, and phenoxyethanol;

[0062] The emulsifier includes one or more of cetearyl alcohol, isoceteth-20, glyceryl stearate, and methyl gluceth-20;

[0063] The penetration enhancer includes pepper extract.

[0064] In the present invention, when the aqueous phase is water, glycerol, butylene glycol, propylene glycol, xanthan gum, carbomer, and chlorphenesin, the mass ratio of water, glycerol, butylene glycol, propylene glycol, xanthan gum, carbomer, and chlorphenesin is 50-70:5-10:5-10:5-10:0.05-0.2:0.1-0.5:0.05-0.15, and is optimized to be 60:12.5:12.5:12.5:0.125:0.3:0.1;

[0065] When the oil phase is hydrogenated polyisobutene, rice bran oil, stearyl glycyrrhetinate, isononyl isononanoate, polydimethylsiloxane, and phenoxyethanol, the mass ratio of hydrogenated polyisobutene, rice bran oil, stearyl glycyrrhetinate, isononyl isononanoate, polydimethylsiloxane, and phenoxyethanol is 1-3:3-8:0.05-0.5:1-8:1-3:0.4-1, preferably 2:5.5:0.275:4.5:2:0.7;

[0066] When the emulsifier is cetearyl alcohol, isoceteth-20, glyceryl stearate, and methyl gluceth-20, the mass ratio of cetearyl alcohol, isoceteth-20, glyceryl stearate, and methyl gluceth-20 is 1-5:0.5-2:1-3:0.5-2, preferably 3:1.25:2:1.25.

[0067] The present invention also provides a method for preparing the cream, comprising the following steps:

[0068] (1) mixing an oil phase and an emulsifier, and heating to obtain a mixture A;

[0069] (2) mixing the mixture A and water, and homogenizing to obtain a mixture B;

[0070] (3) mixing mixture B and a penetration enhancer to obtain a base cream;

[0071] (4) After cooling the base cream to below 45°C, the base cream is mixed with the cold-dampness-removing and body-slimming composition to obtain a cream.

[0072] In the present invention, in step (1), the heating method is iron heating; the heating temperature is 70-90°C, preferably 75-85°C, preferably 80°C.

[0073] In the present invention, in step (2), when the aqueous phase is water, glycerol, butylene glycol, propylene glycol, xanthan gum, carbomer and chlorphenesin, the aqueous phase needs to be dissolved before the mixture A and the aqueous phase are mixed, and the specific steps of dissolving are: mixing water, glycerol, butylene glycol, propylene glycol, xanthan gum, carbomer and chlorphenesin, stirring and heating at a vacuum degree of -0.6 to -0.2 bar and a temperature of 80 to 100° C. for 20 to 40 minutes;

[0074] The stirring is external stirring; the stirring speed is 15 to 35 rpm, preferably 20 to 30 rpm, and more preferably 25 rpm;

[0075] The vacuum degree is preferably -0.5 to -0.3 bar, more preferably -0.4 bar; the temperature is preferably 85 to 95°C, more preferably 90°C; the heating time is preferably 25 to 35 minutes, more preferably 30 minutes;

[0076] The mixing is stirring mixing, and the stirring mixing includes external stirring and internal stirring, and the stirring speed is 5-45rpm, preferably 15-35rpm, and more preferably 25rpm; the external stirring speed is 25-45rpm, preferably 30-40rpm, and more preferably 35rpm; the internal stirring speed is 5-25rpm, preferably 10-20rpm, and more preferably 15rpm;

[0077] The rotation speed of the homogenizer is 2000-3000 rpm, preferably 2300-2700 rpm, and more preferably 2500 rpm; the homogenization time is 2-4 min, and preferably 3 min; the vacuum degree of the homogenizer is -0.7-0.5 bar, preferably -0.75-0.55 bar, and more preferably -0.60 bar.

[0078] In the present invention, the mixing is stirring mixing, and the stirring mixing includes external stirring and internal stirring, and the stirring speed is 5 to 35 rpm, preferably 15 to 25 rpm, and more preferably 20 rpm; the external stirring speed is 15 to 35 rpm, preferably 20 to 30 rpm, and more preferably 25 rpm; the internal stirring speed is 5 to 25 rpm, preferably 10 to 20 rpm, and more preferably 15 rpm;

[0079] The mixing vacuum degree is -0.7 to -0.5 bar, preferably -0.75 to -0.55 bar, and more preferably -0.60 bar.

[0080] In the present invention, in step (4), the temperature is preferably 15 to 35°C, more preferably 25°C; the vacuum degree of the mixing is -0.7 to -0.5 bar, preferably -0.75 to -0.55 bar, more preferably -0.60 bar;

[0081] The mixing is stirring mixing, and the stirring mixing includes external stirring and internal stirring. The stirring speed is 5 to 35 rpm, preferably 15 to 25 rpm, and more preferably 20 rpm; the external stirring speed is 15 to 35 rpm, preferably 20 to 30 rpm, and more preferably 25 rpm; the internal stirring speed is 5 to 25 rpm, preferably 10 to 20 rpm, and more preferably 15 rpm.

[0082] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0083] Embodiment 1 A cream for removing cold and dampness and slimming the body

[0084] (1) 2 g of hydrogenated polyisobutene, 5.5 g of rice bran oil, 0.275 g of stearyl glycyrrhetinate, 4.5 g of isononyl isononanoate, 2 g of dimethicone, 0.7 g of phenoxyethanol, 3 g of cetearyl alcohol, 1.25 g of isoceteth-20, 2 g of glyceryl stearate, and 1.25 g of methyl gluceth-20 were mixed, and a steel pipe was heated to 80° C. to dissolve to obtain a mixture A;

[0085] (2) 60 g of water, 12.5 g of glycerol, 12.5 g of butylene glycol, 12.5 g of propylene glycol, 0.125 g of xanthan gum, 0.3 g of carbomer, and 0.1 g of chlorphenesin were added to an emulsifying pot, mixed and dissolved under external stirring at 25 rpm, vacuum degree -0.4 bar, and temperature 90° C. to obtain an aqueous phase; Mixture A was added to the emulsifying pot containing the aqueous phase, stirred and mixed under external stirring at 25 rpm and internal stirring at 15 rpm, and homogenized at a speed of 2870 rpm and a vacuum degree of -0.64 bar for 3 min to obtain a mixture B;

[0086] (3) adding 0.105 g of pepper extract to the emulsifying pot containing the mixture B, stirring and mixing under external stirring at 25 rpm, internal stirring at 15 rpm and vacuum degree at -0.64 bar to obtain a base cream;

[0087] (4) The base cream was cooled to 45°C, and 1.55 g of lotus leaf extract, 1.05 g of poppy flower extract, 0.055 g of white chamomile flower extract, 1.55 g of Poria cocos extract, 1.55 g of ginger root extract, 1.55 g of cinnamon extract, 0.15 g of patchouli extract, 0.055 g of moringa seed extract, 0.255 g of saffron extract, 0.255 g of mugwort extract, and 0.55 g of functional polypeptide (the mass ratio of collagen amino acids, oligopeptides and heptapeptides was 1:5:2) was added to an emulsifying pot containing the base cream, and the mixture was stirred and mixed under external stirring at 25 rpm, internal stirring at 15 rpm and vacuum at -0.64 bar to obtain a cream.

[0088] Example 2

[0089] (1) 3 g of hydrogenated polyisobutene, 4 g of rice bran oil, 0.5 g of stearyl glycyrrhetinate, 6 g of isononyl isononanoate, 1 g of polydimethylsiloxane, 1 g of phenoxyethanol, 4 g of cetearyl alcohol, 2 g of isoceteth-20, 1 g of glyceryl stearate, and 0.5 g of methyl gluceth-20 were mixed, and an iron tube was heated to 90° C. to dissolve to obtain a mixture A;

[0090] (2) 55 g of water, 8 g of glycerol, 6 g of butylene glycol, 5 g of propylene glycol, 0.5 g of xanthan gum, 0.1 g of carbomer, and 0.15 g of chlorphenesin were added to an emulsifying pot, mixed and dissolved under external stirring at 30 rpm, vacuum degree -0.6 bar, and temperature of 80° C. to obtain an aqueous phase; Mixture A was added to the emulsifying pot containing the aqueous phase, stirred and mixed under external stirring at 40 rpm and internal stirring at 10 rpm, and homogenized at a speed of 3000 rpm and a vacuum degree of -0.70 bar for 2 min to obtain a mixture B;

[0091] (3) adding 0.06 g of pepper extract into the emulsifying pot containing the mixture B, stirring and mixing at 30 rpm externally and 10 rpm internally under a vacuum degree of -0.5 bar to obtain a base cream;

[0092] (4) The base cream was cooled to 30°C, and 1 g of lotus leaf extract, 0.5 g of corn poppy flower extract, 0.1 g of white chamomile flower extract, 3 g of Poria cocos extract, 0.1 g of ginger root extract, 3 g of cinnamon extract, 0.2 g of patchouli extract, 1 g of moringa seed extract, 0.4 g of saffron extract, 0.1 g of mugwort extract, and 0.3 g of functional polypeptide (the mass ratio of collagen amino acids, oligopeptides and heptapeptides is 1:7:2) was added to an emulsifying pot containing the base cream, and the mixture was stirred under external stirring of 15 rpm, internal stirring of 5 rpm and vacuum degree of -0.7 bar to obtain a cream.

[0093] Example 3

[0094] (1) 1.5 g hydrogenated polyisobutene, 7 g rice bran oil, 0.1 g stearyl glycyrrhetinate, 1 g isononyl isononanoate, 3 g dimethicone, 0.5 g phenoxyethanol, 1 g cetearyl alcohol, 0.5 g isoceteth-20, 3 g glyceryl stearate, and 2 g methyl gluceth-20 were mixed, and an iron tube was heated to 70° C. to dissolve to obtain a mixture A;

[0095] (2) 70 g of water, 5 g of glycerol, 10 g of butylene glycol, 9 g of propylene glycol, 0.05 g of xanthan gum, 0.5 g of carbomer, and 0.05 g of chlorphenesin were added to an emulsifying pot, mixed and dissolved under external stirring at 20 rpm, vacuum degree -0.3 bar, and temperature of 100° C. to obtain an aqueous phase; Mixture A was added to the emulsifying pot containing the aqueous phase, stirred and mixed under external stirring at 30 rpm and internal stirring at 20 rpm, and homogenized at a speed of 2500 rpm and a vacuum degree of -0.50 bar for 4 minutes to obtain Mixture B;

[0096] (3) adding 0.014 g of pepper extract into the emulsifying pot containing the mixture A, stirring and mixing under external stirring at 20 rpm, internal stirring at 20 rpm and vacuum degree at -0.7 bar to obtain a base cream;

[0097] (4) The base cream was cooled to 20°C, and 3 g of lotus leaf extract, 2 g of corn poppy flower extract, 0.05 g of white chamomile flower extract, 1 g of Poria cocos extract, 2 g of ginger root extract, 0.1 g of cinnamon extract, 0.1 g of patchouli extract, 0.05 g of moringa seed extract, 0.1 g of saffron extract, 0.5 g of mugwort extract, and 1 g of functional polypeptide (the mass ratio of collagen amino acids, oligopeptides and heptapeptides was 1:3:1) were added to an emulsifying pot containing the base cream, and the mixture was stirred at 30 rpm externally and 20 rpm internally under a vacuum degree of -0.5 bar to obtain a cream.

[0098] Experimental Example 1

[0099] 1. Construction of obese mouse model:

[0100] Twenty SPF-grade C57BL / 6J mice, male, 6 weeks old, weighing 22±2g, were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd. Five mice were fed normally as the blank group, and the remaining 15 mice were fed a high-fat diet for 7 weeks (1 to 7 weeks). They were evenly divided into three groups according to their weight: a control group, a matrix cream group, and a cold-dampness-removing slimming cream group.

[0101] At 2:00 pm every day, water, water, the base cream in step (3) of Example 1, and the cream described in Example 1 were applied to the blank group, control group, base cream group, and cold-dampness-removing slimming cream group, respectively, at 1 g / 5 cm each time. 2 , 5 times a week, for 8 consecutive weeks (7 to 15 weeks).

[0102] 2. Mouse weight analysis:

[0103] The body weight of mice was measured weekly. Figure 1 a and Figure 1 As shown in b; at the 15th week, the mice were killed, the visceral fat was collected and weighed, and the body fat ratio of the mice was calculated. The results are shown in Figure 1 c and Figure 1 As shown in d;

[0104] Body fat ratio = fat weight / body weight*100%.

[0105] from Figure 1 a and Figure 1 As can be seen from Figure b, starting from the 8th week, the weight of mice in the blank group and the control group showed an upward trend, while the weight of mice in the matrix cream group and the cold-dampness-removing and slimming cream group showed a downward trend, and the weight of mice in the cold-dampness-removing and slimming cream group was significantly lower than that in the matrix cream group; it can be seen that the cream described in Example 1 can effectively reduce the weight of obese mice; Figure 1 c and Figure 1As can be seen from Figure d, the fat content and body fat ratio of mice in the cold-dampness-removing and slimming cream group were significantly lower than those of mice in the control group, and the fat content and body fat ratio of mice in the cold-dampness-removing and slimming cream group were equivalent to those of mice in the normally fed blank group; therefore, the cream described in Example 1 can achieve the effect of weight loss by reducing body weight, reducing fat weight and reducing body fat ratio.

[0106] The adipose tissue sections were stained with HE, and the morphology and size of the adipocytes of each group of mice were observed under a microscope. The stained mouse adipocyte photos were quantitatively analyzed using Image-J image processing software. The results are shown in Table 1 and Figure 2 As shown;

[0107] Table 1 Fat area of ​​mice

[0108]

[0109]

[0110] From Table 1 and Figure 2 It can be seen that the fat cells of mice significantly increased after being fed with a high-fat diet (control group), and after applying the cream described in Example 1, the area of ​​fat cells was significantly reduced, indicating that the cream described in Example 1 can reduce the volume of fat cells and play a shaping role.

[0111] 3. Mouse adipokine detection:

[0112] After the mice were killed at week 15, blood was collected from the orbital venous plexus, placed at 25°C for 30 min, placed at 4°C for 1 h, centrifuged at 3000 rpm for 15 min, and the supernatant was collected and the leptin content in the mouse blood was detected using a leptin ELISA kit. The test results are shown in Figure 3 shown.

[0113] Leptin (LP) is a protein hormone secreted by adipose tissue. It can enhance the oxidation of fatty acids and the body's uptake of glucose, while suppressing appetite and stimulating energy consumption, reducing the body's excessive energy intake. In people with obesity and sub-health diseases such as metabolic syndrome, the body's sensitivity to leptin is reduced, resulting in abnormally high leptin levels in the blood, leptin resistance and hyperleptinemia, making leptin unable to play a role in reducing energy intake.

[0114] from Figure 3 It can be seen that the leptin content in the blood of obese mice fed with a high-fat diet is significantly increased. The cream described in Example 1 can effectively reduce the leptin content in the blood of obese mice and help to reduce the occurrence of leptin resistance.

[0115] 4. Triglyceride determination:

[0116] At the 15th week, mice were killed, and blood was collected from the orbital venous plexus, placed at 25°C for 30 minutes, placed at 4°C for 1 hour, and centrifuged at 3000 r / min for 15 minutes. 100 μL of the upper serum was collected and the triglyceride index in the blood lipids was tested using a triglyceride kit (201SJTZ201, purchased from Guangzhou Chichang Trading Company) and an automatic biochemical analyzer. The test results are shown in Figure 4 As shown;

[0117] One of the main functions of fat cells in the body is to store energy, and triglycerides are the main storage form of fat cells. In obesity, the volume of fat cells increases and the amount of triglycerides stored increases. When the body weight is reduced, the triglyceride level tends to decrease. In this experiment, mice were made obese by feeding them a high-fat diet, which led to an increase in triglyceride levels in fat cells in the body, in order to simulate the increase of triglycerides in fat cells in the body under obesity conditions.

[0118] from Figure 4 It can be seen that the cream described in Example 1 can reduce the triglyceride level by promoting fat decomposition, which indicates that the cream described in Example 1 has the potential effect of promoting fat decomposition and reducing triglyceride synthesis.

[0119] 5. Detection of key factors in appetite regulation:

[0120] After the mice were killed at week 15, the back skin tissue of the mice was taken and the cells were lysed with lysis buffer (RIPA): protease inhibitor (PMSF) at a volume ratio of 100:1. The cells were ultrasonically broken at 40 kW for 5 s, with an interval of 10 s for 1 min, shaken for 1 min, and ice-bathed for 3 min. After repeating 10 times, the skin tissue homogenate was obtained and centrifuged at 1000 r / min for 10 min at 4°C. The supernatant was taken. The β-endorphin content in the supernatant was detected by β-endorphin ELISA kit. The test results are shown in Table 1. Figure 5 As shown;

[0121] β-endorphin can suppress appetite, increase the body's energy release, inhibit fat production, and reduce weight; at the same time, β-endorphin is also called the "happy hormone", which makes people feel happy and satisfied; a decrease in the level of β-endorphin in the body can easily lead to anxiety and depression.

[0122] from Figure 5 It can be seen that high-fat diet will lead to a decrease in β-endorphin levels, while the cream described in Example 1 can effectively increase the content of β-endorphin, while reducing fat production and helping to maintain healthy emotions.

[0123] Experimental Example 2 Clinical Trial

[0124] The patients in this experimental example were all provided by beauty salons and health clubs that used the cream for removing cold and dampness and slimming the body as described in Example 1.

[0125] In the Qing Dynasty, Wu Jing pointed out in "Treatise on Warm Diseases·Lower Jiao" that "If dampness is not treated for a long time, it will hide in the foot Shaoyin, cause white tongue and body pain, and edema of the foot and instep... Dampness will damage the yang for a long time, causing weakness and paralysis of limbs, and bleeding from hemorrhoids"; "Huangdi Neijing·Suwen" pointed out that "cold and dampness qi, which is held in the qi exchange, will cause people to suffer from cold and dampness, muscle atrophy, and foot atrophy and inability to contract", "People who are affected by cold and dampness will suffer from body heaviness and swelling, chest and abdominal pain", etc. Therefore, cold and dampness includes two aspects: exogenous cold and dampness and endogenous cold and dampness. Exogenous cold and dampness: exogenous cold and dampness evil qi, the circulation of qi and blood is blocked; endogenous cold and dampness: cold and dampness are trapped inside and damage spleen yang, or spleen and kidney yang are deficient and cold and dampness are stagnant inside. Exogenous cold and dampness are often characterized by pain in joints, tendons and bones; endogenous cold and dampness are often characterized by fear of cold and cold limbs, abdominal pain, diarrhea, or edema.

[0126] Cold and dampness can cause fatigue, drowsiness, fear of cold, cold hands and feet, swollen ankles, chest tightness, poor appetite, abdominal distension, heavy head, heavy body, bland mouth without thirst, scanty urination, swollen limbs (especially lower limbs), palpitations, pain in the joints, skin eczema and ulcers, pale and fat tongue, white and slippery or greasy tongue coating, and a soft and weak pulse.

[0127] 1. Evaluation of the slimming effect before and after using the cold-dampness-removing slimming cream described in Example 1

[0128] Table 2 Changes in body shape

[0129] Name Use area Use times Use front waist circumference Use the back waist Li abdomen 1 time 86.5cm 81.3cm Zhang abdomen 1 time 81.8cm 78.3cm Fu abdomen 1 time 83cm 81cm Wang abdomen 1 time 92.3cm 85.6cm

[0130] It can be seen from Table 2 that the cold-dampness-removing and body-slimming cream described in Example 1 can significantly reduce abdominal circumference and improve abdominal obesity;

[0131] 2. Evaluation of the effect on improving cold and dampness

[0132] Case 1:

[0133] Ms. Shen, 42 years old, had obstructed blood circulation, which led to systemic obesity, meridian lymphatic congestion, thick and loose back, and periarthritis of shoulder. She used the cold and dampness-removing slimming cream described in Example 1 of the present invention for one year, using the trunk and limbs, 100g each time, once a week; overall, the whole body was smaller, the body felt lighter, the back was significantly thinner, the symptoms of obesity and weight bearing were weakened, and the body shape and posture were significantly improved; shoulder discomfort was significantly improved, and periarthritis of shoulder was improved; the skin and muscles were also tighter, and the body and complexion were significantly improved.

[0134] Case 2:

[0135] Ms. Zhang, 38 years old, had cold abdomen, fear of cold and cold limbs, and had problems such as bloating and stool retention all year round, and edema. After using the cold-dampness-removing and slimming cream described in Example 1 once, the abdomen was tightened by 2.5cm, the skin of the body was firm, the edema was significantly improved, and the body shape and posture became better; a large amount of oily substances were discharged, the abdominal bloating was significantly improved, and there was a warm feeling, and the stool retention was also discharged smoothly.

[0136] Case 3:

[0137] Ms. Li, 34 years old, usually sits for a long time, and her popliteal fossa is blocked and swollen, her calves are obviously swollen, and the temperature of the lower part of the body is low. She is afraid of the cold, has cold hands and feet, and feels that her legs are heavy and weak. After using the cream for expelling cold and dampness and slimming the body described in Example 1 once, the swelling problem was significantly improved, the heaviness of the legs was greatly reduced, the leg circumference became significantly thinner, and the leg shape was well improved; the cold hands and feet and fear of the cold were improved.

[0138] Case 4:

[0139] Ms. Shao, 40 years old, an office white-collar worker, often sits for long periods of time to work overtime, has edema, soreness, swelling and numbness in the legs; after using 50g of the cold-dampness-removing and slimming cream described in Example 1 on the left leg, the edema was significantly reduced, the body shape and posture improved, the skin became tighter and it was also much more relaxed. The comparison of the left and right legs is as follows: Figure 6 shown.

[0140] Case 5:

[0141] Ms. Chen, 33 years old, was afraid of cold, had cold hands and feet, obvious joint pain when squatting, calf soreness, and small granular nodules. She said that every time she used the cold-dampness-removing and slimming cream described in Example 1, her calves felt warm and comfortable, the pain when squatting was significantly relieved, the pain in the joints and calves was significantly improved, the soreness and swelling in the legs was also improved, the leg skin was tighter, the edema was improved, walking was much easier, and the body shape was well improved.

[0142] It can be seen from the above embodiments that the present invention provides a composition for expelling cold and dampness and slimming the body and its application. The cold and dampness slimming cream of the present invention has the following effects: it can reduce body weight, reduce fat weight and reduce body fat ratio; it can reduce the size of fat cells, thereby playing a firming effect; it can regulate leptin and help reduce the occurrence of leptin resistance; it can promote fat decomposition and reduce triglyceride synthesis; it can increase endorphins and improve mood; in the specific use process, it can effectively improve body shape and posture, and can also effectively improve health problems caused by dampness and cold such as abdominal obesity and lower limb edema.

[0143] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A composition for removing cold and dampness and slimming the body, characterized in that: The invention comprises the following components in parts by weight: 0.1 to 3 parts of lotus leaf extract, 0.1 to 2 parts of poppy flower extract, 0.01 to 0.1 parts of white chamomile flower extract, 0.1 to 3 parts of poria extract, 0.1 to 3 parts of ginger root extract, 0.1 to 3 parts of cinnamon extract, 0.1 to 0.2 parts of patchouli extract, 0.01 to 1 parts of moringa seed extract, 0.01 to 0.5 parts of saffron extract, 0.01 to 0.5 parts of wormwood extract and 0.1 to 1 parts of functional polypeptide.

2. The composition according to claim 1, characterized in that The functional polypeptide includes one or more of collagen amino acids, oligopeptides and heptapeptides.

3. Use of the composition according to claim 1 or 2 in the preparation of a cold-dampness-expelling and body-slimming preparation.

4. A cream for removing cold and dampness and slimming the body, characterized in that: The composition comprises the following components in parts by weight: 65-101 parts of water phase, 7-24 parts of oil phase, 3-12 parts of emulsifier, 0.01-0.2 parts of penetration enhancer, and 0.4-16 parts of cold-dampness-removing and body-slimming composition; The composition for expelling cold and dampness and slimming the body is the composition according to claim 1 or 2.

5. The cream according to claim 4, characterized in that The aqueous phase includes one or more of water, glycerin, butylene glycol, propylene glycol, xanthan gum, carbomer, and chlorphenesin; The oil phase comprises one or more of hydrogenated polyisobutene, rice bran oil, stearyl glycyrrhetinate, isononyl isononanoate, polydimethylsiloxane, and phenoxyethanol; The emulsifier includes one or more of cetearyl alcohol, isoceteth-20, glyceryl stearate, and methyl gluceth-20; The penetration enhancer includes pepper extract.

6. The method for preparing the cream according to claim 4 or 5, characterized in that: The steps include: (1) mixing an oil phase and an emulsifier, and heating to obtain a mixture A; (2) mixing the mixture A and water, and homogenizing to obtain a mixture B; (3) mixing mixture B and a penetration enhancer to obtain a base cream; (4) After cooling the base cream to below 45°C, the base cream is mixed with the cold-dampness-removing and body-slimming composition to obtain a cream.

7. The preparation method according to claim 6, characterized in that: In step (1), the heating temperature is 70-90°C.

8. The preparation method according to claim 7, characterized in that: In step (2), the mixing is stirring mixing, and the stirring speed is 5 to 45 rpm; The rotation speed of the homogenizer is 2000-3000 rpm; the homogenization time is 2-4 min; and the vacuum degree of the homogenizer is -0.7-0.5 bar.

9. The preparation method according to claim 8, characterized in that: In step (3), the mixing is stirring mixing, and the stirring speed is 5 to 35 rpm; the vacuum degree of the mixing is -0.7 to -0.5 bar.

10. The preparation method according to claim 9, characterized in that: In step (4), the mixing is stirring mixing, and the stirring speed is 5 to 35 rpm; the vacuum degree of the mixing is -0.7 to -0.5 bar.

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