Ultrathin high-strength nanofiber medicinal casing and preparation method thereof

Abstract

The invention belongs to the technical field of medical materials, and particularly relates to an ultrathin high-strength nanofiber medicinal casing and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a pretreatment raw material; (2) adding a compound enzyme into the pretreated raw material for enzymolysis, performing enzymolysis at 35-40 DEG C for 5-8 hours, and performing enzyme deactivation to obtain an enzymolysis product; (3) adding compound bacteria into the enzymolysis product for anaerobic fermentation, fermenting at 35-40 DEG C for 10-12 hours, and sterilizing to obtain a fermentation product; (4) washing the fermentation product with clear water for 5-8 times to obtain a casing semi-finished product; and (5) mixing nano cellulose, soybean lecithin and water to prepare a feed liquid, spraying the semi-finished sausage casing product with the feed liquid, and drying to obtain the ultra-thin high-strength nano-fiber medicinal sausage casing. The casing prepared by the method can be used for wrapping a traditional Chinese medicine composition.

Description

Technical Field

[0001] The invention belongs to the technical field of medical materials, and in particular relates to an ultra-thin and high-strength nanofiber medicinal casing and a preparation method thereof. Background Art

[0002] Due to their diverse ingredients and complex preparation processes, Chinese medicine compositions often face inconvenient packaging and sales. Traditional Chinese medicine preparations such as decoctions and powders are difficult to accurately control the dosage of each dose, which increases the complexity and uncertainty of use. Some consumers are not very accepting of the appearance and method of taking traditional Chinese medicine (such as decoction), especially the younger generation who prefer convenient and fast modern medicine forms.

[0003] After the Chinese medicine composition is wrapped in a plastic packaging bag, when it is unpacked and taken, it is usually in the form of solid particles or powder, which is easy to cause waste due to improper operation, causing inconvenience to the patient. When opening the packaging bag, solid particles or powdered Chinese medicine are easy to spill due to slight shaking or tipping. Especially during the movement process (such as carrying out), the packaging bag is more likely to be damaged or improperly opened, causing the medicine to spill. The spilling of medicine in the external environment is not only a waste, but may also be contaminated, affecting the efficacy and even posing a health hazard. For special groups such as the elderly or children, the inconvenience in operation is more obvious.

[0004] In order to solve the above problems, we can consider using casings for packaging. Casings can form an effective barrier to protect the Chinese medicine ingredients from the external environment, such as moisture-proof and anti-oxidation, thereby extending the shelf life and maintaining the efficacy. It helps to enhance consumers' trust and willingness to buy, and is especially suitable for international market promotion.

[0005] However, using sheep intestines to wrap Chinese medicines may indeed bring a series of technical challenges and potential problems. The following are some of the main technical problems and their possible impact on the efficacy of the medicine: too much residue on the surface of the sheep intestines, too thick thickness affects the taste; it may also cause adverse reactions. Natural sheep intestines are thicker, especially when they are not treated, which will significantly affect the taste when taken and increase the difficulty of swallowing. The sheep intestines themselves have a unique animal smell, which may be difficult for some patients to accept, especially when they need to be taken for a long time.

[0006] Therefore, there is an urgent need for an ultra-thin and high-strength nanofiber medicinal casing and a preparation method thereof. Summary of the invention

[0007] The purpose of the present invention is to provide an ultra-thin and high-strength nanofiber medicinal casing and a preparation method thereof

[0008] In order to achieve the above object, the present invention provides the following technical solutions:

[0009] A method for preparing an ultra-thin and high-strength nanofiber medicinal casing comprises the following steps:

[0010] (1) soaking the sheep intestine in warm water at 25-30° C. for 20-25 hours, placing the soaked sheep intestine on a flat wooden board with the inner wall facing outward, scraping off the mucosal layer and muscle layer of the intestine with a intestine scraper, rinsing the scraped casing with water, and then evenly sprinkling refined salt on the casing, pickling for 10-15 hours, and washing with clean water to obtain a pretreated raw material;

[0011] (2) adding a composite enzyme to the pretreated raw material for enzymolysis at 35-40° C. for 5-8 hours, inactivating the enzyme, and obtaining an enzymolysis product;

[0012] (3) adding composite bacteria to the enzymatic hydrolyzate for anaerobic fermentation at 35-40° C. for 10-12 h, sterilizing, and obtaining a fermentation product;

[0013] (4) washing the fermented product with clean water for 5 to 8 times to obtain a semi-finished casing;

[0014] (5) Nanocellulose, soybean lecithin and water are mixed to prepare a liquid feed, and the liquid feed is sprayed on the semi-finished casing, and then dried to obtain an ultra-thin and high-strength nanofiber medicinal casing.

[0015] Furthermore, in step (1), the weight ratio of sheep intestine to warm water is 1:(10-15).

[0016] Further, the complex enzyme includes papain, ficin and bromelain;

[0017] Furthermore, the amount of papain used in the pretreated raw material is 100-110 U / mL; the amount of ficin used in the pretreated raw material is 120-130 U / mL; and the amount of bromelain used in the pretreated raw material is 80-90 U / mL.

[0018] The present invention uses enzymes that are able to break down protein components in the sheep intestine, especially those parts that are tightly bound to impurities. This helps to loosen and remove impurities that are attached to the surface of the sheep intestine or embedded in its structure. The enzymes gradually break down the connective tissue in the sheep intestine, making it softer and easier to handle. This not only facilitates the subsequent fermentation steps, but also reduces the risk of mechanical damage. Through enzymatic hydrolysis, many impurities on the surface and inside the sheep intestine are broken down into smaller molecules, which are easier to wash away, thereby improving the overall cleanliness.

[0019] Furthermore, the composite bacteria include Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium longum.

[0020] Furthermore, the dosage of Lactobacillus casei is 10 7 -10 8CFU / mL pre-treated raw materials; the amount of Lactobacillus plantarum was 10 9 -10 10 CFU / mL pre-treated raw materials; the dosage of Bifidobacterium longum was 10 8 -10 9 CFU / mL pre-treated raw material.

[0021] Further, 1 part by weight of nanocellulose, 4-5 parts by weight of soybean lecithin and 100 parts by weight are mixed to prepare a slurry.

[0022] During the anaerobic fermentation process, the complex bacteria will produce various metabolites, such as organic acids and enzymes, which can further degrade the remaining macromolecules, including incompletely decomposed proteins, polysaccharides and lipids. The organic acids (such as lactic acid) produced during the fermentation process can create an acidic environment, which helps dissolve certain minerals and impurities and further purify the sheep intestines.

[0023] Through the dual effects of enzymatic hydrolysis and fermentation, non-essential components in the sheep intestine are gradually removed, resulting in a significant reduction in its thickness. This is crucial for the manufacture of ultra-thin and high-strength nanofiber medicinal casings. The present invention forms a reinforced film on the surface of the sheep intestine by spraying and drying nanocellulose and soybean lecithin, thereby improving the overall tensile strength.

[0024] Further, drying is performed at 80-85° C. to a moisture content of 7-9%.

[0025] Furthermore, the amount of spraying is 50-60g / m 2 .

[0026] The invention provides an ultra-thin and high-strength nanofiber medicinal enteric casing prepared by the preparation method.

[0027] The method for using the medicinal enteric casing of the present invention is as follows: wrapping the Chinese medicine composition with the enteric casing, then sealing it with an outer package, storing it in a refrigerator, and directly taking it after opening the outer package when taking it.

[0028] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0029] 1. The present invention sequentially performs enzymolysis and fermentation to remove impurities on the sheep intestine and reduce the thickness of the casing. Through enzymolysis, many impurities on the surface and inside of the sheep intestine are decomposed into smaller molecules, which are easier to be washed away, thereby improving the overall cleanliness. The composite bacteria will produce various metabolites, such as organic acids, enzymes, etc., which can further degrade the remaining macromolecular substances, including incompletely decomposed proteins, polysaccharides and lipids.

[0030] 2. The spraying and drying process of nanocellulose and soybean lecithin can form a reinforced film on the surface of goat intestine, thereby improving the overall tensile strength. Nanocellulose has excellent mechanical properties and high specific surface area, and can form a strong and thin protective layer on the surface of goat intestine, significantly improving tensile strength and durability. Soybean lecithin, as a natural amphiphilic molecule, can increase flexibility and biocompatibility. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The raw materials used in the following examples of the present invention are all commercially available products:

[0033] Bromelain, enzyme activity 1200u / mg. Shanghai Yuanye Biotechnology Co., Ltd.

[0034] Papain, enzyme activity 800u / mg. Shanghai Yuanye Biotechnology Co., Ltd.

[0035] Fig enzyme, enzyme activity 100u / mg. Shanghai Yuanye Biotechnology Co., Ltd.

[0036] Lactobacillus casei, product number CICC 25035, China Industrial Microbiological Culture Collection Center.

[0037] Lactobacillus plantarum, product number CCTCC AB 2022356, China Center for Type Culture Collection.

[0038] Bifidobacterium longum, product number CICC 25033, China Industrial Microbiological Culture Collection Center.

[0039] Nanocellulose, Hubei Xinyuhong Biopharmaceutical Technology Co., Ltd.

[0040] Soybean lecithin, Guangdong Mingcheng Biotechnology Co., Ltd.

[0041] Example 1

[0042] This embodiment provides a method for preparing an ultra-thin and high-strength nanofiber medicinal casing, comprising the following steps:

[0043] (1) soaking the sheep intestine in warm water at 28° C. for 24 hours, placing the soaked sheep intestine on a flat wooden board with the inner wall facing outward, scraping off the mucosal layer and muscle layer of the intestine with a intestine scraper, rinsing the scraped casing with water, and then evenly sprinkling refined salt on the casing, pickling for 12 hours, and washing with clean water to obtain a pretreated raw material;

[0044] (2) adding a composite enzyme to the pretreated raw material for enzymolysis, performing enzymolysis at 37° C. for 6 hours, inactivating the enzyme, and obtaining an enzymolysis product; the composite enzyme comprises papain, ficin, and bromelain; the amount of papain in the pretreated raw material is 105 U / mL; the amount of ficin in the pretreated raw material is 125 U / mL; and the amount of bromelain in the pretreated raw material is 85 U / mL.

[0045] (3) Adding composite bacteria to the enzymatic hydrolyzate for anaerobic fermentation at 36°C for 11 hours, sterilizing, and obtaining a fermentation product; the composite bacteria include Lactobacillus casei, Lactobacillus plantarum, and Bifidobacterium longum. The amount of Lactobacillus casei is 10 7 CFU / mL pre-treated raw materials; the amount of Lactobacillus plantarum was 10 10 CFU / mL pre-treated raw materials; the dosage of Bifidobacterium longum was 10 9 CFU / mL pre-treated raw material.

[0046] (4) washing the fermented product with clean water seven times to obtain a semi-finished casing;

[0047] (5) 1 part by weight of nanocellulose, 5 parts by weight of soybean lecithin and 100 parts by weight of cellulose were mixed to prepare a liquid; the liquid was sprayed on the semi-finished casing, and the amount of the liquid sprayed was 55 g / m 2 , dried at 83°C to a moisture content of 8%; an ultra-thin and high-strength nanofiber medicinal casing was obtained.

[0048] Example 2

[0049] This embodiment provides a method for preparing an ultra-thin and high-strength nanofiber medicinal casing, comprising the following steps:

[0050] (1) Soaking the sheep intestine in warm water at 25° C. for 20 hours, placing the soaked sheep intestine on a flat wooden board with the inner wall facing outward, scraping off the mucosal layer and muscle layer of the intestine with a intestine scraper, rinsing the scraped casing with water, and then evenly sprinkling refined salt on the casing, pickling for 15 hours, and washing with clean water to obtain a pretreated raw material;

[0051] (2) adding a composite enzyme to the pretreated raw material for enzymolysis at 40° C. for 8 h, inactivating the enzyme, and obtaining an enzymolysis product; the composite enzyme comprises papain, ficin and bromelain; the amount of papain in the pretreated raw material is 100 U / mL; the amount of ficin in the pretreated raw material is 130 U / mL; and the amount of bromelain in the pretreated raw material is 80 U / mL.

[0052] (3) Adding composite bacteria to the enzymatic hydrolyzate for anaerobic fermentation at 40° C. for 10 h, sterilizing, and obtaining a fermentation product; the composite bacteria include Lactobacillus casei, Lactobacillus plantarum, and Bifidobacterium longum. The amount of Lactobacillus casei is 10 8 CFU / mL pre-treated raw materials; the amount of Lactobacillus plantarum was 10 9 CFU / mL pre-treated raw materials; the dosage of Bifidobacterium longum was 10 9 CFU / mL pre-treated raw material.

[0053] (4) washing the fermented product with clean water 8 times to obtain a semi-finished casing;

[0054] (5) 1 part by weight of nanocellulose, 4 parts by weight of soybean lecithin and 100 parts by weight of cellulose were mixed to prepare a liquid; the liquid was sprayed on the semi-finished casing, and the amount of the liquid sprayed was 50 g / m 2 , dried at 85°C to a moisture content of 9%; an ultra-thin and high-strength nanofiber medicinal casing was obtained.

[0055] Comparative Example 1

[0056] The difference between this comparative example and Example 1 is that no fermentation is performed.

[0057] Comparative Example 2

[0058] The difference between this comparative example and Example 1 is that no enzymatic hydrolysis is performed.

[0059] Comparative Example 3

[0060] The difference between this comparative example and Example 1 is that the complex enzyme is replaced by ficin.

[0061] Comparative Example 4

[0062] The difference between this comparative example and Example 1 is that the amount of papain used in the pretreated raw material is 85 U / mL; the amount of ficin used in the pretreated raw material is 105 U / mL; and the amount of bromelain used in the pretreated raw material is 125 U / mL.

[0063] Comparative Example 5

[0064] The difference between this comparative example and Example 1 is that the composite bacteria is replaced by Lactobacillus casei.

[0065] Comparative Example 6

[0066] The difference between this comparative example and Example 1 is that the amount of Lactobacillus casei is 10 10 CFU / mL pre-treated raw materials; the amount of Lactobacillus plantarum was 10 7 CFU / mL pre-treated raw materials; the dosage of Bifidobacterium longum was 10 10 CFU / mL pre-treated raw material.

[0067] Performance Testing

[0068] The performance of the medicinal casings prepared in Examples 1-2 and Comparative Examples 1-6 was tested. The tensile strength of the single-layer medicinal casings was measured using a universal material testing machine. The thickness of the casings was measured using a micrometer.

[0069] Table 1 Performance test results

[0070]

[0071]

[0072] It can be seen from the above performance test results that by controlling appropriate fermentation and enzymatic hydrolysis conditions, the medicinal enteric casings of Examples 1-2 still maintain good strength, are thinner, and have no peculiar smell.

[0073] In Comparative Example 1, no fermentation was performed, and in Comparative Example 2, no enzymatic hydrolysis was performed. The thickness of the medicinal casing increased and there was an odor.

[0074] In Comparative Example 3, a single enzyme was used, the thickness of the medicinal enteric casing increased, and there was also an odor.

[0075] In Comparative Example 4, the amount of enzyme used was different, and the thickness of the medicinal casing increased.

[0076] In Comparative Example 5, a single bacteria fermentation was used, and the thickness of the medicinal casing increased and there was an odor.

[0077] In Comparative Example 6, different amounts of bacteria were used, and the thickness of the medicinal enteric casing increased.

[0078] The above experimental results further prove the importance of the technical solution defined in the present invention to its technical effect.

[0079] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for preparing an ultra-thin and high-strength nanofiber medicinal casing, characterized in that: The following steps are involved: (1) soaking the sheep intestine in warm water at 25-30° C. for 20-25 hours, placing the soaked sheep intestine on a flat wooden board with the inner wall facing outward, scraping off the mucosal layer and muscle layer of the intestine with a intestine scraper, rinsing the scraped casing with water, and then evenly sprinkling refined salt on the casing, pickling for 10-15 hours, and washing with clean water to obtain a pretreated raw material; (2) adding a composite enzyme to the pretreated raw material for enzymolysis at 35-40° C. for 5-8 hours, inactivating the enzyme, and obtaining an enzymolysis product; (3) adding composite bacteria to the enzymatic hydrolyzate for anaerobic fermentation at 35-40° C. for 10-12 h, sterilizing, and obtaining a fermentation product; (4) washing the fermented product with clean water for 5 to 8 times to obtain a semi-finished casing; (5) Nanocellulose, soybean lecithin and water are mixed to prepare a liquid feed, and the liquid feed is sprayed on the semi-finished casing, and then dried to obtain an ultra-thin and high-strength nanofiber medicinal casing.

2. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 1, characterized in that: In the step (1), the weight ratio of sheep intestine to warm water is 1:(10-15).

3. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 1, characterized in that: The complex enzyme comprises papain, ficin and bromelain.

4. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 3, characterized in that: The dosage of papain in the pretreated raw material is 100-110 U / mL; the dosage of ficin in the pretreated raw material is 120-130 U / mL; and the dosage of bromelain in the pretreated raw material is 80-90 U / mL.

5. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 1, characterized in that: The complex bacteria include Lactobacillus casei, Lactobacillus plantarum and Bifidobacterium longum.

6. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 5, characterized in that: The dosage of the lactobacillus casei is 10 7 -10 8 CFU / mL pre-treated raw materials; the amount of Lactobacillus plantarum was 10 9 -10 10 CFU / mL pre-treated raw materials; the dosage of Bifidobacterium longum was 10 8 -10 9 CFU / mL pre-treated raw material.

7. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 1, characterized in that: 1 part by weight of nanocellulose, 4-5 parts by weight of soybean lecithin and 100 parts by weight are mixed to prepare a feed liquid.

8. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 1, characterized in that: Dry at 80-85℃ to a moisture content of 7-9%.

9. The method for preparing the ultra-thin and high-strength nanofiber medicinal casing according to claim 1, characterized in that: The usage of spraying is 50-60g / m 2 .

10. An ultra-thin and high-strength nanofiber medicinal casing prepared according to the preparation method according to any one of claims 1 to 9.

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