Fertilizer for optimizing soil phosphorus content and / or promoting plant growth and application thereof
By combining inorganic phosphorus fertilizer with brown algae fermentation, the phosphorus absorption of plants is regulated, and the problem of difficult absorption of insoluble phosphorus fertilizers in the soil is solved, the optimization utilization of soil phosphorus and the promotion of plant growth is achieved, the use of chemical phosphorus fertilizers is reduced, and the risk of environmental pollution is reduced.
Patent Information
- Application Number
- CN202510173867.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-17
- Publication Date
- 2025-05-23
AI Technical Summary
In the prior art, insoluble inorganic phosphorus fertilizers in the soil are difficult to absorb by plants, resulting in low agricultural production efficiency, and excessive use of chemical phosphorus fertilizers leads to environmental pollution and reduced soil fertility.
A fertilizer is used to combine inorganic phosphorus fertilizer and brown algae fermentation, and the fermentation obtained after fermentation of Bacillus fermentation of seashore regulating phosphorus absorption in plants, promoting plant growth, and optimizing soil phosphorus content.
It effectively improves the biomass and phosphorus absorption efficiency of plants, optimizes the utilization ratio of soil phosphorus, reduces the use of chemical phosphorus fertilizers, and reduces the risk of environmental pollution.
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Figure CN120025211A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of fertilizers, and in particular relates to a fertilizer for optimizing soil phosphorus content and / or promoting plant growth and application thereof. Background Art
[0002] Corn is one of the most important C4 food crops in the world. Its efficient growth and development requires sufficient nutrient supply, especially phosphorus, a key nutrient. Phosphorus directly affects plant growth, development, and crop yield and quality. However, most of the phosphorus in the soil exists mainly in the form of insoluble inorganic phosphorus, which is difficult for plants to absorb. This problem seriously limits agricultural production efficiency.
[0003] In order to make up for the shortage of phosphorus supply, chemically synthesized inorganic phosphorus fertilizers are widely used in traditional agriculture. However, the long-term excessive application of chemical phosphorus fertilizers has led to a series of environmental problems, including: (1) environmental pollution: phosphorus loss causes eutrophication of water bodies and damages the ecosystem; (2) soil phosphorus accumulation: phosphorus fertilizers are fixed in the soil, reducing soil productivity. Therefore, it is particularly important to find a new method to improve the effectiveness of soil phosphorus and reduce dependence on chemical fertilizers to promote plant growth. Summary of the invention
[0004] The purpose of the present invention is to make up for the deficiencies of the prior art, effectively regulate the phosphorus absorption of plants, promote plant growth, optimize the phosphorus content in the soil, reduce the use of chemical phosphorus fertilizers, and reduce the risk of environmental pollution while increasing production and efficiency.
[0005] The present invention provides a fertilizer, comprising an inorganic phosphorus fertilizer and a fermented brown algae;
[0006] The mass of the inorganic phosphate fertilizer and the brown algae fermentation product is measured in terms of P, and the mass ratio of the inorganic phosphate fertilizer to the brown algae fermentation product in the fertilizer is 24.76:5.24;
[0007] The brown algae fermentation product includes a fermentation product obtained by fermenting brown algae with Bacillus litoralis.
[0008] Preferably, the fertilizer is in the form of an aqueous solution.
[0009] Preferably, the masses of the inorganic phosphate fertilizer and the brown algae fermentation product are measured in terms of P, the concentration of the inorganic phosphate fertilizer in the fertilizer is 24.76 mg / kg, and the concentration of the brown algae fermentation product in the fertilizer is 5.24 mg / kg.
[0010] Preferably, the method for preparing the brown algae fermentation product comprises the following steps:
[0011] Inoculating Bacillus marinatus into a mixture of brown algae powder and water, and collecting the supernatant after solid-liquid separation to obtain the brown algae fermentation product;
[0012] The mass volume ratio of the brown algae powder and water is 1 g:20 mL;
[0013] The pH of the fermentation is 6.5-7.5, the fermentation time is 10-16 hours, and the temperature is 30°C.
[0014] Preferably, the inorganic phosphate fertilizer comprises potassium dihydrogen phosphate.
[0015] The present invention also provides the use of the fertilizer described in the above technical solution in optimizing soil phosphorus content and / or promoting plant growth.
[0016] Preferably, the optimization of soil phosphorus content includes increasing the proportion of soil available phosphorus and / or reducing the proportion of soil fixed phosphorus;
[0017] The promoting plant growth comprises one or more of increasing the aboveground biomass of the plant, increasing the root biomass of the plant, and increasing the phosphorus absorption efficiency of the plant.
[0018] The present invention also provides a method for optimizing soil phosphorus content and / or promoting plant growth, comprising the following steps: applying the fertilizer described in the above technical solution as a base;
[0019] The mass of the fertilizer is measured in P, and the basic application rate of the fertilizer is 30 to 150 mg / kg of soil.
[0020] Preferably, the method further comprises: applying topdressing;
[0021] The topdressing comprises inorganic phosphate fertilizer or brown algae fermentation product; the brown algae fermentation product comprises the brown algae fermentation product in the fertilizer described in the above technical solution;
[0022] The mass of the topdressing is measured in P, and the total amount of the topdressing applied is 2.62-5.24 mg / kg of soil.
[0023] Preferably, the topdressing method is root irrigation; the time for topdressing is the three-leaf stage of the plant, the number of topdressing is 5 times, and the time interval between two adjacent topdressing is 3 days.
[0024] Beneficial effects:
[0025] The present invention provides a fertilizer, including an inorganic phosphate fertilizer and a brown algae fermentation product; the mass of the inorganic phosphate fertilizer and the brown algae fermentation product is measured in P, and the mass ratio of the inorganic phosphate fertilizer to the brown algae fermentation product in the fertilizer is 24.76:5.24; the brown algae fermentation product includes a fermentation product obtained by fermenting brown algae with Bacillus maritime. The inorganic phosphate fertilizer can provide inorganic phosphorus required for plant growth; the brown algae fermentation product is rich in a variety of biologically active substances, which can improve the effectiveness of phosphorus and improve the soil environment. The present invention combines the inorganic phosphate fertilizer and the brown algae fermentation product, which can synergistically enhance the efficiency, effectively regulate the phosphorus absorption of plants, promote plant growth, and optimize the phosphorus content in the soil.
[0026] Furthermore, the present invention uses the above fertilizer as base fertilizer and combines it with topdressing to achieve the synergistic optimization of plant growth and soil phosphorus utilization, which can not only improve crop yield and quality, but also significantly reduce the use of chemical phosphorus fertilizers, thereby reducing the risk of environmental pollution, and has important ecological and economic value. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.
[0028] Figure 1 This is the effect of different concentrations of brown algae fermentation products on corn growth and phosphorus absorption in Example 2;
[0029] Figure 2 The effect of topdressing brown algae fermentation on corn growth and phosphorus absorption under different phosphorus levels in Example 3;
[0030] Figure 3 The effect of topdressing brown algae fermentation product on soil Olsen-P content under different phosphorus levels in Example 4;
[0031] Figure 4 This is the effect of applying brown algae fermentation products on soil inorganic phosphorus fractionation under different phosphorus levels in Example 4;
[0032] Figure 5 This is the effect of applying brown algae fermentation product on corn growth and phosphorus absorption under different phosphorus levels in Example 5;
[0033] Figure 6 This is the effect of applying brown algae fermentation product on soil Olsen-P content and inorganic phosphorus fractionation under different phosphorus levels in Example 5. DETAILED DESCRIPTION
[0034] The present invention provides a fertilizer, comprising an inorganic phosphorus fertilizer and a fermented brown algae;
[0035] The mass of the inorganic phosphate fertilizer and the brown algae fermentation product is measured in terms of P, and the mass ratio of the inorganic phosphate fertilizer to the brown algae fermentation product in the fertilizer is 24.76:5.24;
[0036] The brown algae fermentation product includes a fermentation product obtained by fermenting brown algae with Bacillus litoralis.
[0037] As an embodiment, the dosage form of the fertilizer is an aqueous solution. As an embodiment, the mass of the inorganic phosphate fertilizer is measured in P, and the concentration of the inorganic phosphate fertilizer in the fertilizer is 24.76 mg / kg. As an embodiment, the mass of the brown algae fermentation product is measured in P, and the concentration of the brown algae fermentation product in the fertilizer is 5.24 mg / kg.
[0038] As an embodiment, the inorganic phosphate fertilizer includes potassium dihydrogen phosphate. As an embodiment, the mass ratio of potassium dihydrogen phosphate to brown algae fermentation product in the fertilizer is 108.79:250.
[0039] As an embodiment, the total phosphorus content in the brown algae fermentation is 20.96 mg / g. The present invention has no strict requirements on the source of the brown algae fermentation, which can be prepared by itself or purchased conventionally, such as the brown algae fermentation of Wuzhoufeng Agricultural Technology Co., Ltd. As an embodiment, the preparation method of the brown algae fermentation comprises the following steps: inoculating Bacillus maritime in a mixture of brown algae powder and water, collecting the supernatant after solid-liquid separation after fermentation, and obtaining the brown algae fermentation. As an embodiment, the mass volume ratio of the brown algae powder and water is 1g:20mL. As an embodiment, the pH of the fermentation is 6.5-7.5; as another embodiment, the pH of the fermentation is 7.0. As an embodiment, the fermentation time is 10-16h; as another embodiment, the fermentation time is 12-15h. As an embodiment, the fermentation temperature is 30°C; as another embodiment, the fermentation temperature is 30°C. The present invention has no strict requirements on the source of the Bacillus maritime, and conventional purchase is sufficient.
[0040] Inorganic phosphorus fertilizer can provide inorganic phosphorus required for plant growth; brown algae fermentation products are rich in a variety of biologically active substances, which can improve the effectiveness of phosphorus and improve the soil environment. The present invention combines inorganic phosphorus fertilizers and brown algae fermentation products, which can synergistically enhance the efficiency, effectively regulate plant phosphorus absorption, promote plant growth, and optimize soil phosphorus content. Therefore, the use of the fertilizer in optimizing soil phosphorus content and / or promoting plant growth also belongs to the protection scope of the present invention. As an embodiment, the soil includes calcareous tidal soil. As an embodiment, the optimization of soil phosphorus content includes increasing the proportion of soil available phosphorus and / or reducing the proportion of soil fixed phosphorus. As an embodiment, the promotion of plant growth includes increasing the aboveground biomass of plants, increasing the root biomass of plants, and improving the phosphorus absorption efficiency of plants. As another embodiment, the promotion of plant growth includes increasing the aboveground biomass of plants, increasing the root biomass of plants, and improving the phosphorus absorption efficiency of plants.
[0041] The present invention also provides a method for optimizing soil phosphorus content and / or promoting plant growth, comprising the following steps: applying the fertilizer described in the above technical solution as a basic application; the mass of the fertilizer is measured in P, and the basic application amount of the fertilizer is 30 to 150 mg / kg of soil.
[0042] As an embodiment, the mass of the fertilizer is measured in terms of P, and the basal application rate of the fertilizer is 30 mg / kg soil. The present invention can optimize the soil phosphorus content, significantly increase the biomass and phosphorus absorption of plants, significantly increase the phosphorus concentration of the aboveground part and root system of plants, and improve the phosphorus absorption efficiency of plants by basal application of a compound of brown algae fermentation product and potassium dihydrogen phosphate.
[0043] As an embodiment, after the fertilizer described in the above technical solution is applied as a base, topdressing is applied. As an embodiment, the topdressing of the present invention includes inorganic phosphate fertilizer or brown algae fermentation; the brown algae fermentation includes a fermentation obtained by fermenting brown algae with Bacillus maritime. The preparation method of the brown algae fermentation of the present invention is consistent with the above, and will not be repeated here. As an embodiment, the mass of the topdressing of the present invention is measured in P, and the total amount of the topdressing applied is 2.62-5.24 mg / kg soil; as another embodiment, the mass of the topdressing of the present invention is measured in P, and the total amount of the topdressing applied is 5.24 mg / kg soil. As an embodiment, the method of topdressing is root irrigation. As an embodiment, the time of the topdressing is the three-leaf stage of the plant. As an embodiment, the number of topdressing is 5 times. As an embodiment, the time interval between two adjacent topdressing is 3d. As an embodiment, the amount of topdressing applied between two adjacent topdressing is 1:1.
[0044] As an embodiment, when the topdressing is brown algae fermentation product, the brown algae fermentation product is diluted according to the applied amount and then irrigated. As an embodiment, the concentration of the brown algae fermentation product in the dilution of the brown algae fermentation product is 0.5-1 mg / mL; as another embodiment, the concentration of the brown algae fermentation product in the dilution of the brown algae fermentation product is 1 mg / mL. As an embodiment, each time the topdressing is performed, the root irrigation amount of the dilution of the brown algae fermentation product is 100 mL per pot, with 2 plants per pot. The present invention has no strict requirements on the method of root irrigation, and the dilution of the brown algae fermentation product can be evenly and slowly poured into the soil around the base of the plant stem.
[0045] As an embodiment, when the topdressing is an inorganic phosphate fertilizer, the inorganic phosphate fertilizer is diluted according to the applied amount and then irrigated. As an embodiment, the inorganic phosphate fertilizer is potassium dihydrogen phosphate, and the concentration of potassium dihydrogen phosphate in the dilution of potassium dihydrogen phosphate is 0.0924 mg / mL. As another embodiment, each time the topdressing is performed, the root irrigation amount of the dilution of potassium dihydrogen phosphate is 100 mL / pot. The present invention has no strict requirements on the method of root irrigation, and the dilution of potassium dihydrogen phosphate can be evenly and slowly poured into the soil around the base of the plant stem.
[0046] The present invention applies brown algae fermentation product or potassium dihydrogen phosphate as a topdressing on the basis of the basal application of potassium dihydrogen phosphate and the brown algae fermentation product compound, thereby further increasing the content of soil available phosphorus, improving the soil phosphorus classification ratio, and contributing to long-term improvement of soil fertility.
[0047] In order to further illustrate the present invention, a fertilizer for optimizing soil phosphorus content and / or promoting plant growth and its application provided by the present invention are described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the scope of protection of the present invention.
[0048] Example 1
[0049] The preparation of brown algae fermentation products is as follows: First, clean the fresh copper algae (Sargassum (Bactrophycus) horneri (Turner) C. Agar), dry it at 60°C after drying, and then crush the copper algae into 40 mesh fine powder and mix it with distilled water at a mass volume ratio of 1g:20mL to make a mixed solution. On this basis, adjust the pH value to 6.5-7.5 and sterilize it at 121°C. After the mixed solution is cooled to room temperature, inoculate Bacillus litoralis and ferment it at 30°C for 10-16h. After the fermentation is completed, the fermentation liquid is obtained by centrifugation, which is the brown algae fermentation product.
[0050] Example 2
[0051] Effects of Brown Algae Fermentation on Phosphorus Absorption of Corn
[0052] 1. Preparation of brown algae fermentation product mother liquor: The brown algae fermentation product obtained in Example 1 and water were mixed evenly to obtain a brown algae fermentation product mother liquor with a concentration of 0.1 g / mL.
[0053] 2. In the corn pot experiment, P (KH 2 PO 4 ); When corn was in the three-leaf stage, the rats were randomly divided into a control group, an experimental group 1 and an experimental group 2, and the following treatments were performed:
[0054] Control group (LP-CK): no topdressing;
[0055] Experimental group 1 (LP-C1): The mother solution of brown algae fermentation was diluted 200 times to obtain a 0.5 mg / mL dilution; 100 mL of the dilution was applied to each corn pot once every 3 days and evenly applied to the soil around the base of the corn, for a total of 5 times;
[0056] Experimental group 2 (LP-C2): The mother solution of brown algae fermentation was diluted 100 times to obtain a 1.0 mg / mL dilution. 100 mL of the dilution was applied to each pot of corn and evenly applied to the soil around the base of the corn. The application was once every 3 days for a total of 5 times.
[0057] Each treatment group was carried out in the greenhouse of China Agricultural University, and the harvest time was 56 days. When the corn matured, the aboveground and root biomass of corn in each treatment group, as well as the phosphorus absorption of corn, were counted. The results showed that the addition of brown algae fermentation products could significantly increase the aboveground and root biomass of corn, especially at a concentration of 1.0 mg / mL, the aboveground biomass increased by 46.11%, and the root biomass increased by 35.69% ( Figure 1 The phosphorus concentrations in the aboveground and root systems increased significantly by 130.78% and 144.36%, respectively ( Figure 1 The phosphorus uptake of corn increased significantly with the increase of fermentation product concentration ( Figure 1 (C)).
[0058] Example 3
[0059] Effects of topdressing brown algae fermentation products on corn growth and phosphorus absorption under different phosphorus levels
[0060] 1. Preparation of brown algae fermentation product dilution: dilute the brown algae fermentation product mother solution of Example 2 100 times to obtain a 1.0 mg / mL brown algae fermentation product dilution.
[0061] 2. Two phosphorus levels were set in the corn pot experiment: low phosphorus (30 mg P per kg soil base) and high phosphorus (150 mg / kg P per kg soil base), and the effects of topdressing 1.0 mg / mL brown algae fermentation on corn growth and phosphorus absorption were compared. The experiment was divided into four treatment groups: (1) LP-M-C0: low phosphorus condition, no fermentation was added; (2) LP-M-C1: low phosphorus condition, when corn was in the three-leaf stage, 100 mL of brown algae fermentation diluted solution was applied to each corn pot once every 3 days, evenly applied to the soil around the base of the corn, for a total of 5 times; (3) HP-M-C0: high phosphorus condition, no fermentation was added; (4) HP-M-C1: high phosphorus condition, when corn was in the three-leaf stage, 100 mL of brown algae fermentation diluted solution was applied to each corn pot once every 3 days, evenly applied to the soil around the base of the corn, for a total of 5 times.
[0062] 3. Each treatment group was carried out in the greenhouse of China Agricultural University. When the corn matured, the aboveground and root biomass of the corn in each treatment group, as well as the phosphorus absorption of the corn, were counted. The results showed that under low-phosphorus conditions, the application of brown algae fermentation products significantly increased the aboveground and root biomass of corn. In contrast, under high-phosphorus conditions, there were no significant differences among the treatment groups. This shows that the promoting effect of brown algae fermentation products on plant growth is more significant under low-phosphorus conditions. Under low-phosphorus conditions (LP), compared with no addition of brown algae fermentation products (C0), the addition of brown algae fermentation products can significantly increase the aboveground biomass by 39.67% and the root biomass by 48.45%, while there was no significant difference under high-phosphorus conditions (HP) ( Figure 2 In (A) and (B)). Under low-phosphorus and high-phosphorus conditions, there was no significant difference in phosphorus concentration in the aboveground parts and roots after adding brown algae fermentation products ( Figure 2 (C) and (D)). The effect of brown algae fermentation products on phosphorus uptake was consistent with that of biomass. Under low phosphorus conditions (LP), the addition of brown algae fermentation products significantly increased phosphorus uptake by 31.22% aboveground and 86.19% root systems compared with no addition of brown algae fermentation products ( Figure 2 In summary, under low phosphorus conditions (LP), the addition of brown algae fermentation products promoted the growth of corn and improved the phosphorus nutritional status of corn, which is consistent with the results of Example 2.
[0063] Example 4
[0064] 1. Preparation of brown algae fermentation product dilution: dilute the brown algae fermentation product mother solution of Example 2 by 100 times to obtain a 1.0 mg / mL brown algae fermentation product dilution.
[0065] 2. Two phosphorus levels were set in the pot experiment: low phosphorus (30 mg P per kg soil base) and high phosphorus (150 mg / kg P per kg soil base), and the effects of topdressing 1.0 mg / mL brown algae fermentation on the growth and phosphorus absorption of corn were compared. The experiment was divided into eight treatment groups: (1) LP-M-C0: low phosphorus conditions, corn was planted without adding fermentation; (2) LP-M-C1: low phosphorus conditions, corn was planted, and when the corn was in the three-leaf stage, 100 mL of the diluted brown algae fermentation solution was applied to each pot of corn once every 3 days, and evenly applied to the soil around the base of the corn, for a total of 5 times; (3) LP-NM-C0: low phosphorus conditions, no corn was planted, and no fermentation was added; (4) LP-NM-C1: low phosphorus conditions, no corn was planted, and when the corn was in the three-leaf stage, 100 mL of the diluted brown algae fermentation solution was applied to each pot of corn once every 3 days, and evenly applied to the soil around the base of the corn. , a total of 5 times; (5) HP-M-C0: high phosphorus conditions, corn was planted without adding fermented materials; (6) HP-M-C1: high phosphorus conditions, corn was planted, and when the corn was in the three-leaf stage, 100 mL of brown algae fermented material diluted solution was applied to each pot of corn once every 3 days, and the solution was evenly applied to the soil around the base of the corn, for a total of 5 times; (7) HP-NM-C0: high phosphorus conditions, no corn was planted, and no fermented materials were added; (8) HP-NM-C1: high phosphorus conditions, no corn was planted, and when the corn was in the three-leaf stage, 100 mL of brown algae fermented material diluted solution was applied to each pot of corn once every 3 days, and the solution was evenly applied to the soil around the base of the corn, for a total of 5 times.
[0066] 3. Effects of topdressing brown algae fermentation products on soil available phosphorus (Olsen-P) content under different phosphorus levels
[0067] Step 2 After the corn in each treatment group matures, the available phosphorus content in the soil is detected with reference to the existing technology (Olsen SR. Estimation of available phosphorus in soils by extraction with sodium bicarbonate [M]. USDepartment of Agriculture, 1954.). The results show that topdressing with brown algae fermentation products can increase the content of available phosphorus in the soil, which is beneficial to the phosphorus absorption of subsequent crops. Under low-phosphorus conditions, the two factors of corn growth and the addition of brown algae fermentation products have no interactive effect on the Olsen-P content. The main factor affecting the Olsen-P content is the brown algae fermentation product. Regardless of whether corn is planted or not, the addition of brown algae fermentation products under low-phosphorus conditions can significantly increase the Olsen-P content in the soil compared with not adding brown algae fermentation products, with an increase of 63% and 47.61% respectively. Under high-phosphorus conditions, the addition of brown algae fermentation products has no significant effect on the Olsen-P content in the soil ( Figure 3 )
[0068] 4. Effect of topdressing brown algae fermentation products on the proportion of various forms of inorganic phosphorus in soil under different phosphorus levels
[0069] Step 2 After the corn in each treatment group matured, the proportion of various forms of inorganic phosphorus in calcareous fluvo-aquic soil was detected according to the existing technology (Jiang Baifan, Gu Yichu. Research on inorganic phosphorus classification system in calcareous soil [J]. Chinese Agricultural Science, 1989, 1(03): 58-66.). The results showed that under low phosphorus conditions, adding brown algae fermentation products would increase the Ca content in the soil. 2 -P ratio. Without corn, Ca 2- The percentage of P increased from 4.98% to 6.83% in the case of corn, while it increased from 4.90% to 7.35% in the case of corn. In contrast, under low-P conditions, the addition of brown algae fermentation products reduced the Ca content in the soil. 8 -P content. Under high phosphorus conditions, adding brown algae fermentation products has an effect on soil Ca 2 -P content percentage and Ca 8 The percentage of Al-P content was the same as that of Fe-P content. After adding brown algae fermentation products, the percentage content of Al-P and Fe-P remained relatively stable. Under low phosphorus conditions, the percentage content of Al-P and Fe-P in corn growth conditions was basically 8.85% and 10.39%, respectively, while under high phosphorus conditions, it was 14.66% and 13.97%, respectively. The OP content ratio was also relatively stable, ranging from 11.45% to 12.41% under low phosphorus conditions and 12.24% to 13.64% under high phosphorus conditions ( Figure 4 In conclusion, the addition of brown algae fermentation products increased soil Ca 2 -P ratio, reducing the Ca content in the soil 8 -P ratio, which helps to improve the availability of phosphorus, especially under low phosphorus conditions. Therefore, under this treatment, phosphorus is more easily available, which may promote the growth and increase the yield of corn. At the same time, the addition of brown algae fermentation products showed a certain improvement effect under different phosphorus supply levels, indicating that it has potential in optimizing soil phosphorus supply.
[0070] Example 5
[0071] 1. Preparation of brown algae fermentation product dilution: dilute the brown algae fermentation product mother solution of Example 2 by 100 times to obtain a 1.0 mg / mL brown algae fermentation product dilution.
[0072] Preparation of mixed fertilizer solution: KH 2 PO 4 and brown algae fermentation product were dissolved in 100 mL of deionized water at a mass ratio of 108.79:250 to obtain a mixed fertilizer solution with a brown algae fermentation product concentration of 2.5 mg / mL.
[0073] KH 2 PO 4 Solution preparation: KH 2 PO 4 Mix with water to prepare 0.0924 mg / mL KH 2 PO 4 Solution.
[0074] 2. Taking potted corn as an example, 2 seedlings were planted in each pot, and the corn was harvested after 56 days of growth. The experiment was divided into six treatment groups:
[0075] (1) P-0: KH only 2 PO 4 ;
[0076] (2)PP: Base KH 2 PO 4 , topdressing KH 2 PO 4 ;
[0077] (3) PA: KH 2 PO 4 , topdressing with brown algae fermentation products;
[0078] (4) PA-0: KH 2 PO 4 A mixed fertilizer solution obtained by mixing with brown algae fermentation product;
[0079] (5) PA-A: Base application of mixed fertilizer solution, topdressing of brown algae fermentation product,
[0080] (6) PA-P: Base application of mixed fertilizer solution, topdressing with KH 2 PO 4 , the specific processing methods are as follows:
[0081] P-0: KH 2 PO 4 As a base fertilizer, the application rate was 131.77 mg / kg (30 mg / kg P); no topdressing was performed;
[0082] PP: KH 2 PO 4 As a base fertilizer, the application rate is 131.77 mg / kg (30 mg / kg P); when corn is in the three-leaf stage, each pot of corn is applied with KH obtained in step 1 every 3 days. 2 PO 4 The solution was evenly applied to the soil around the base of the corn for a total of 5 times;
[0083] PA: KH 2 PO 4As a base fertilizer, the application amount is 131.77 mg / kg (30 mg / kg P); when the corn is in the three-leaf stage, each pot of corn is applied with the dilution of the brown algae fermentation product obtained in step 1 once every 3 days, for a total of 5 times;
[0084] PA-0: The mixed fertilizer solution obtained in step 1 was used as base fertilizer, and 100 mL was applied to each pot of corn; no topdressing was performed;
[0085] PA-A: The mixed fertilizer solution obtained in step 1 was used as base fertilizer, and 100 mL was applied to each pot of corn. When the corn was in the three-leaf stage, the diluted solution of brown algae fermentation obtained in step 1 was applied to each pot of corn once every 3 days, for a total of 5 times.
[0086] PA-P: Use the mixed fertilizer solution obtained in step 1 as base fertilizer, and apply 100 mL to each pot of corn; when the corn is in the three-leaf stage, apply the KH obtained in step 1 to each pot of corn once every 3 days. 2 PO 4 Apply the solution evenly to the soil around the base of the corn for a total of 5 times.
[0087] 3. When the corn in each treatment group matured, the aboveground and root biomass of the corn in each treatment group, as well as the phosphorus absorption of the corn, were counted. The results are as follows: Figure 5 As shown. Figure 5 It can be seen that, compared with only KH 2 PO 4 Compared with the treatment of basal application and topdressing, the treatment of basal application and topdressing significantly increased the phosphorus content in the aboveground and root parts of maize. 2 PO 4 In the case of (P-0, PP, PA), the application of KH 2 PO 4 and brown algae fermentation products increased the aboveground biomass by 12.83% and 31.02%, and the root biomass by 32.37% and 40.66%, respectively. The topdressing of brown algae fermentation products increased the aboveground phosphorus uptake by 44.52% and the root phosphorus uptake by 42.85%, respectively. 2 PO 4 : Brown algae fermentation product (m / m) = 108.79:250) (PA-0), with the base fertilizer application of KH 2 PO 4 Compared with (P-0), basal application of mixed fertilizer solution can significantly increase the aboveground biomass of corn by 57.54% and the aboveground phosphorus content by 62.0%. 2 PO 4 Under the same conditions, 2 PO 4 (PP) compared to KH 2 PO 4and brown algae fermentation product (PA-P) can significantly increase the aboveground biomass of corn by 30.95% and the aboveground phosphorus content by 37.28%. 2 PO 4 (PP) compared to KH 2 PO 4 The results showed that the fermented brown algae (PA-P) could significantly increase the aboveground biomass of corn by 44.54% and the aboveground phosphorus content by 60.13%. 2 PO 4 Mixed application, and the ratio is KH 2 PO 4 :Brown algae fermentation product (m / m) = 108.79:250, it can promote the growth of corn and improve corn phosphorus absorption. 2 PO 4 With brown algae fermentation (KH 2 PO 4 : brown algae fermentation (m / m) = 108.79:250) (PA-0, PA-P, PA-A), compared with no topdressing, topdressing with brown algae fermentation significantly increased the aboveground biomass by 20.21% and the root biomass by 32.04%, and increased the aboveground phosphorus content by 42.85% and the root phosphorus content by 119.58%; compared with topdressing with KH 2 PO 4 In comparison, topdressing of brown algae fermentation product significantly increased the aboveground biomass by 28.17% and the aboveground phosphorus content by 40.18%.
[0088] 4. When the corn in each treatment group was mature, the content of available phosphorus (Olsen-P) in the soil and the proportion of various forms of inorganic phosphorus were detected in accordance with the method of Example 4. The results are as follows: Figure 6 As shown. Figure 6 It can be seen that topdressing brown algae fermentation products can significantly increase the Olsen-P content in the soil and regulate the inorganic phosphorus content of each grade in the soil. The test results are consistent with the results of Example 2 under the condition of 30 mg / kg P, which showed that topdressing brown algae fermentation products has a significant effect on improving corn phosphorus absorption and regulating soil phosphorus content. 2 PO 4 Compared with the control group, the topdressing of brown algae fermentation significantly increased the content of Ca2-P, Ca8-P and Fe-P in the soil. 2 PO 4 Mixed application, and the ratio is KH 2 PO 4 :Brown algae fermentation product (m / m) = 108.79:250, can increase the available phosphorus (Ca2 -P) ratio and reduce fixed phosphorus (Ca 8 -P) ratio, effectively improving the utilization rate of phosphorus.
[0089] According to the above content, it can be seen that the technical solution provided by the present invention can promote corn growth, significantly increase corn biomass, increase corn phosphorus absorption, regulate the phosphorus content in the soil, and improve phosphorus utilization, ultimately achieving the purpose of increasing production and efficiency. In addition, the present invention effectively reduces the application amount of chemical phosphorus fertilizers, reduces the risk of eutrophication of water bodies caused by phosphorus loss, and reduces the damage of agriculture to the ecosystem by improving the soil environment.
[0090] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.
Claims
1. A fertilizer, characterized in that: Including inorganic phosphate fertilizers and brown algae fermentation products; The mass of the inorganic phosphate fertilizer and the brown algae fermentation product is measured in terms of P, and the mass ratio of the inorganic phosphate fertilizer to the brown algae fermentation product in the fertilizer is 24.76:5.24; The brown algae fermentation product includes a fermentation product obtained by fermenting brown algae with Bacillus litoralis.
2. The fertilizer according to claim 1, characterized in that The fertilizer is in the form of an aqueous solution.
3. The fertilizer according to claim 2, characterized in that The masses of the inorganic phosphate fertilizer and the brown algae fermentation product are measured in terms of P. The concentration of the inorganic phosphate fertilizer in the fertilizer is 24.76 mg / kg, and the concentration of the brown algae fermentation product in the fertilizer is 5.24 mg / kg.
4. The fertilizer according to any one of claims 1 to 3, characterized in that The method for preparing the brown algae fermentation product comprises the following steps: Inoculating Bacillus marinatus into a mixture of brown algae powder and water, and collecting the supernatant after solid-liquid separation to obtain the brown algae fermentation product; The mass volume ratio of the brown algae powder and water is 1 g:20 mL; The pH of the fermentation is 6.5-7.5, the fermentation time is 10-16 hours, and the temperature is 30°C.
5. The fertilizer according to any one of claims 1 to 3, characterized in that The inorganic phosphate fertilizer includes potassium dihydrogen phosphate.
6. Use of the fertilizer according to any one of claims 1 to 5 in optimizing soil phosphorus content and / or promoting plant growth.
7. The use according to claim 6, characterized in that: Optimizing soil phosphorus content includes increasing the proportion of soil available phosphorus and / or reducing the proportion of soil fixed phosphorus; The promoting plant growth comprises one or more of increasing the aboveground biomass of the plant, increasing the root biomass of the plant, and increasing the phosphorus absorption efficiency of the plant.
8. A method for optimizing soil phosphorus content and / or promoting plant growth, characterized in that: The method comprises the following steps: applying the fertilizer according to any one of claims 1 to 5 as a base; The mass of the fertilizer is measured in P, and the basic application rate of the fertilizer is 30 to 150 mg / kg of soil.
9. The method according to claim 8, characterized in that The method further comprises: applying topdressing; The topdressing comprises inorganic phosphate fertilizer or brown algae fermentation product; the brown algae fermentation product is the brown algae fermentation product in the fertilizer according to any one of claims 1 to 5; The mass of the topdressing is measured in P, and the total amount of the topdressing applied is 2.62-5.24 mg / kg of soil.
10. The method according to claim 9, characterized in that The method of topdressing is root irrigation; the time of topdressing is the three-leaf stage of the plant, the number of topdressing is 5 times, and the time interval between two adjacent topdressing is 3 days.