Sample test card

Abstract

The invention relates to the field of analytical instruments, in particular to a sample test card. The invention provides a sample test card. The sample test card comprises a first channel, a second channel, a first electrode located in the first channel, a second electrode located in the second channel, a salt bridge and an adsorption element, the first electrode is used for acquiring electrochemical parameters of a sample in the first channel, and the second electrode is used for acquiring electrochemical parameters of a reference solution in the second channel; one end of the salt bridge is exposed in the first channel, and the other end is exposed in the second channel; the adsorption element is arranged adjacent to the salt bridge. The salt bridge can be used for conducting the first electrode and the second electrode, so that a conducting loop is formed under the condition that liquid in the two channels does not make direct contact, the adsorption element is used for helping the liquid make contact with the salt bridge, and the accuracy of measuring the electrochemical parameters of a sample is effectively improved.

Description

Technical Field

[0001] The present application relates to the field of analytical instruments, and in particular to a sample test card. Background Art

[0002] In order to detect biochemical samples, a number of electrodes may be provided in the sample test card to detect the sample using an electrochemical method. Therefore, a conductive circuit needs to be formed between the electrodes in the test card in order to test the electrochemical parameters of the sample.

[0003] At present, in such sample test cards, a permeable membrane is often set between a reference cell with a reference electrode and a measuring channel with a measuring electrode, or the reference solution in the reference cell is directly in contact with the sample in the measuring channel, so as to achieve circuit conduction. However, using these methods will cause the components of the reference solution and the sample to be mixed with each other, which is not conducive to the stability of the measurement environment, and thus will affect the accuracy of the measurement results. In addition, when the reference solution and the sample are in direct contact for measurement, the liquid junction potential between the two is difficult to balance and eliminate; at the same time, the liquid may not be in good contact with each other when filled into the sample test card. These factors will affect the accuracy of the measurement. Summary of the invention

[0004] The technical problem to be solved by the present application is how to provide a sample test card to improve the accuracy of measuring the electrochemical parameters of the sample.

[0005] The present application provides a sample test card, comprising a first channel, a second channel, a first electrode located in the first channel, a second electrode located in the second channel, a salt bridge, and an adsorption element. The first electrode is used to obtain the electrochemical parameters of the sample in the first channel, and the second electrode is used to obtain the electrochemical parameters of the reference solution in the second channel; one end of the salt bridge is exposed in the first channel, and the other end is exposed in the second channel; and the adsorption element is arranged adjacent to the salt bridge.

[0006] Different from the prior art, the present application discloses a sample test card, which realizes conduction between the first electrode and the second electrode in the two channels by setting a salt bridge between a first channel containing a sample and a second channel containing a reference solution, thereby forming a conductive circuit without direct contact between the liquids in the two channels, effectively avoiding mutual doping and contamination of different liquids; at the same time, the almost identical migration rates of anions and cations in the salt bridge are utilized, and an adsorption element arranged adjacent to the salt bridge is utilized to help the salt bridge contact the liquid, thereby effectively reducing the influence of the liquid junction potential on the measurement results of the electrochemical parameters, ensuring the stability of the reference solution and the sample, and improving the accuracy of measuring the electrochemical parameters of the sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0007] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:

[0008] Figure 1 It is a schematic diagram of the assembly structure of an embodiment of a sample test card of the present application.

[0009] Figure 2 yes Figure 1 Schematic diagram of the explosion structure of the sample test card.

[0010] Figure 3 yes Figure 1 Schematic diagram of the assembly structure of the first plate and the second plate.

[0011] Figure 4 yes Figure 1 Schematic diagram of the structure of the first plate.

[0012] Figure 5 yes Figure 1 Schematic diagram of the assembly structure of the first plate and the second plate at another angle.

[0013] Figure 6 yes Figure 5 Schematic diagram of the structure after the D area is enlarged.

[0014] Figure 7 yes Figure 1 Schematic diagram of the cross-sectional structure of the sample test card from another angle.

[0015] Figure 8 yes Figure 7 Schematic diagram of the structure after the B area is enlarged.

[0016] Fig. 9 yes Figure 1 Schematic diagram of the explosion structure of the sample test card from another angle. DETAILED DESCRIPTION

[0017] In order to make the above-mentioned purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are described in detail below in conjunction with the accompanying drawings. It is to be understood that the specific embodiments described herein are only used to explain the present application, rather than to limit the present application. It should also be noted that, for ease of description, only some structures related to the present application are shown in the accompanying drawings, rather than all structures. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of this application.

[0018] The terms "first", "second", etc. in this application are used to distinguish different objects, rather than to describe a specific order. In addition, the terms "including" and "provided with" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, system, product or device that includes a series of steps or units is not limited to the listed steps or units, but optionally includes steps or units that are not listed, or optionally includes other steps or units inherent to these processes, methods, products or devices.

[0019] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0020] With the development of instrumental analysis technology, test cards are usually used to test biochemical samples, so as to improve the test efficiency while keeping the samples from being contaminated, thereby ensuring the accuracy of the test. These sample test cards can detect samples by measuring electrochemical parameters, especially in test cards used for blood gas analyzers, which can form a circuit with a three-electrode system of a reference electrode, an ion electrode and a counter electrode to test samples.

[0021] In earlier schemes, when achieving electrical connection between a reference cell provided with a reference electrode and a measuring channel provided with an ion electrode and a counter electrode, a permeable membrane can be set between the reference cell and the measuring channel, or electrical connection can be achieved by direct contact between a reference liquid in the reference cell and the liquid in the measuring channel.

[0022] However, during the measurement process, when the reference cell and the measuring channel are in contact and connected, that is, when the liquid in the reference cell is connected to the liquid in the measuring channel, and two electrolytes with different compositions or activities are in contact, the positive and negative ions diffuse at the interface of the solution, and the different ion migration speeds through the interface cause the separation of positive and negative charges to form a double electric layer. The potential difference generated in this way is called the liquid junction diffusion potential, or liquid junction potential for short. The liquid junction potential will affect the measurement results, so in the actual measurement process, the liquid junction potential and impedance between the two channels need to be as small as possible. At the same time, the electrode also needs a stable environment when measuring electrochemical parameters, and the sample to be measured should not be contaminated as much as possible. Therefore, the volume, concentration and material composition of the reference solution and the liquid in the measuring channel also need to remain stable. Therefore, when the electrode in the reference cell and the electrode in the measuring channel are connected, the liquid in the measuring channel needs to not be mixed and contaminated with the liquid in the reference cell.

[0023] In the previous technology, whether it is through the form of setting a permeable membrane between the reference cell and the measurement channel, or through the reference liquid in the reference cell directly contacting the liquid in the measurement channel to achieve the conduction between the electrodes, the above requirements cannot be well met, which will have an adverse effect on the accuracy of electrochemical parameter detection. In addition, considering the complex structure of the sample test card, when the two channels are filled with liquid, the circuit may not be well conducted due to structural limitations. For example, the turbulence caused by the structure forms bubbles on the liquid contact surface, affecting the exchange of ions and the conduction of the circuit, which will have a certain impact on the accuracy of sample detection.

[0024] In order to ensure the accuracy of sample detection, the present application provides a sample test card 9, such as Figures 1 to 3 As shown, Figure 1 It is a schematic diagram of the assembly structure of an embodiment of a sample test card of the present application. Figure 2 yes Figure 1 Schematic diagram of the explosion structure of the sample test card. Figure 3 yes Figure 1 Schematic diagram of the assembly structure of the first plate and the second plate. The present application provides a sample test card 9, which includes a first channel 1, a second channel 2, a first electrode 41 located in the first channel 1, and a second electrode 42 located in the second channel 2. Among them, the first electrode 41 is configured to obtain the electrochemical parameters of the sample in the first channel 1, and the second electrode 42 is configured to obtain the electrochemical parameters of the reference solution in the second channel 2. In order to achieve conduction between the first electrode 41 and the second electrode 42, the sample test card 9 also includes a salt bridge 3, one end of the salt bridge 3 is exposed in the first channel 1, and the other end is exposed in the second channel 2. In order to facilitate the contact between the liquid and the salt bridge 3, an adsorption element 56 is also arranged adjacent to the salt bridge 3.

[0025] Among them, the adsorption element 56 can be used to adsorb the liquid filled in the first channel 1 and / or the second channel 2 to the vicinity of the salt bridge 3, so as to squeeze out the bubbles that may be produced on the contact surface between the salt bridge 3 and the liquid, and avoid the bubbles from affecting the conduction between the first electrode 41 and the second electrode 42.

[0026] This embodiment can achieve conduction between the first electrode 41 and the second electrode 42 in the two channels by setting a salt bridge 3 between the first channel 1 containing the sample and the second channel 2 containing the reference solution, thereby forming a conductive circuit without direct contact between the liquids in the two channels, effectively avoiding mutual doping and contamination of different liquids; at the same time, the characteristics of the migration rate of anions and cations in the salt bridge 3 are utilized to effectively reduce the influence of the liquid junction potential on the measurement results of the electrochemical parameters, and the stability of the reference solution and the sample can be ensured, and the adsorption element 56 is added to help the salt bridge 3 contact with the liquid, thereby improving the accuracy of measuring the electrochemical parameters of the sample.

[0027] Optionally, the adsorption element 56 can be wetted by the liquid in the first channel 1 and / or the second channel 2 .

[0028] Optionally, at least one of the adsorption element 56 and the salt bridge 3 can expand after being wetted by the liquid and absorbing the liquid. When the adsorption element 56 is not wetted and / or the salt bridge 3 is not wetted, the adsorption element 56 and the salt bridge 3 are spaced apart; when the adsorption element 56 is wetted and / or the salt bridge 3 is wetted, at least one of the adsorption element 56 and the salt bridge 3 expands so that the two are in contact.

[0029] Optionally, the sample test card 9 may further include a second shell 7 to achieve the overall assembly and fixation of the sample test card 9, so as to press the first plate 4 from the side of the first plate 4 away from the second plate 5, so that the first plate 4, the second plate 5, and the first shell 6 are tightly fixed, thereby avoiding leakage of liquid in the first channel 1 and the second channel 2, which may cause inaccurate measurement results or damage to instruments and equipment.

[0030] Optionally, in the three-electrode system, the first electrode 41 disposed in the measurement channel 11 of the first channel 1 may include a counter electrode 412 and an ion electrode 411. The second electrode 42 disposed in the second channel 2 may be a reference electrode. Thus, the counter electrode 412, the ion electrode 411 and the reference electrode form a three-electrode system for testing the sample under the conduction effect of the salt bridge 3.

[0031] The counter electrode 412 is used to form a loop with the ion electrode 411, so that the current on the ion electrode 411 is unblocked, so as to ensure that the reaction of testing the sample occurs on the ion electrode 411, so that the sample in the first channel 1 can be tested. The reference electrode is also used to conduct with the ion electrode 411 through the salt bridge 3 to form a loop, so as to provide a reference electrochemical parameter in the reference solution of the second channel 2 to measure the electrochemical parameter at the ion electrode 411.

[0032] Alternatively, if Figure 3 As shown, the counter electrode 412 may be disposed at a position in the measurement channel 11 that is closest to the salt bridge 3 , and a plurality of ion electrodes 411 may be arranged along the measurement channel 11 .

[0033] Optionally, since it is difficult for ions migrated from the salt bridge 3 to quickly achieve uniform distribution in the channel, changes in ion concentration in the local area near the salt bridge 3 may cause inaccurate electrode measurement results. Therefore, the second electrode 42 in the second channel 2 can be arranged at a position close to the salt bridge 3 but still maintaining a certain distance from the salt bridge 3.

[0034] Optionally, considering the actual measurement process, the liquid in the measurement channel 11 may need to be replaced, for example, calibration liquid, cleaning liquid and other liquids are introduced into the measurement channel 11 to complete the measurement preparation work before the sample is introduced. However, each time the liquid is emptied, the circuit of the first electrode 41 and the second electrode 42 may be difficult to conduct due to the lack of medium. When the liquid is replaced, the circuit is repeatedly turned on and off, which is not conducive to the stability of the electrochemical measurement system and will have an adverse effect on the measurement results. Therefore, the counter electrode 412 in the measurement channel 11 needs to be set at a position very close to the salt bridge 3, so that when the liquid in the measurement channel 11 is discharged, a small amount of liquid is used to form a liquid film that conducts the salt bridge 3 and the first electrode 41 to maintain the conduction of the three-electrode system circuit.

[0035] Optionally, in order to avoid leakage causing failure of the circuit in the first plate 4, the first electrode 41 in the first channel 1 is also not in direct contact with the salt bridge 3. At the same time, the surface between the first plate 4 and the first channel 1 and the second channel 2 can also be provided with a coating to better protect the circuit structure in the first plate 4.

[0036] Optionally, a third electrode 8 may be further provided on the sample test card 9. There are a plurality of third electrodes 8, which are respectively connected to the first electrodes 41 and the second electrodes 42, so as to obtain the electrochemical parameters measured by the first electrodes 41 and the second electrodes 42, and output them to a medical testing device (not shown) equipped with the sample test card 9. The medical testing device can use the output electrochemical parameters to analyze the properties of the sample such as the composition to obtain the measurement results of the sample.

[0037] Optionally, since the sample passed into the sample test card 9 may be a biochemical sample collected from an organism, in order to ensure that the sample does not deteriorate as much as possible during the test, a temperature measuring component 45 may be provided on the first plate 4 to measure the temperature inside the sample test card 9 .

[0038] Optionally, the temperature measuring component 45 can be a thermistor protruding toward the first channel 1 relative to the first plate 4. A second recess 57 can be provided at a corresponding position on the second plate 5 to avoid the thermistor and ensure the sealing performance of the sample test card 9. Figure 3 As shown, the temperature measuring component 45 can be arranged near the edge of the second plate 5, so that the second recessed portion 57 can be directly recessed from the edge of the second plate 5 to the inside of the second plate 5 for molding processing.

[0039] See also Figures 2 to 4 , Figure 4 yes Figure 1Schematic diagram of the structure of the first plate in the test card. It can be found that due to the structural limitations of the first channel 1 and the second channel 2 in the test card in this embodiment, in order to facilitate the layout of the first electrode 41, the surface area of ​​the portion of the salt bridge 3 exposed in the first channel 1 can be smaller than the surface area of ​​the portion of the salt bridge 3 exposed in the second channel 2. Optionally, the length of the portion of the salt bridge 3 exposed in the first channel 1 can also be smaller than the length of the portion of the salt bridge 3 exposed in the second channel 2. In this way, the salt bridge 3 can avoid the electrode while conducting the circuit to achieve a good measurement effect.

[0040] Since a large amount of liquid is usually required in the second channel 2 to provide a stable test environment for testing the sample, the circuit structure set up in the first channel 1 for testing the sample will also be more complicated. Therefore, in order to leave sufficient space for the first electrode 41 and the arranged circuit structure in the first channel 1, the space reserved for placing the salt bridge in the second channel 2 is generally larger than the space reserved for placing the salt bridge in the first channel 1, and the length or surface area of ​​the salt bridge 3 set in the second channel 2 is also longer or larger than the length or surface area of ​​the salt bridge 3 set in the first channel 1.

[0041] Optionally, for the convenience of laying out the salt bridge 3 and the stability of the measurement system, the salt bridge 3 may be a solid salt bridge 3 made of a hydrogel containing electrolytes, wherein the hydrogel may be agar, gelatinized starch or other materials.

[0042] Optionally, the composition of the electrolyte contained in the salt bridge 3 and the composition of the liquids passing into the first channel 1 and the second channel 2 can match each other, so that the electrolyte composition of the salt bridge 3 and the liquids in the first channel 1 and the second channel 2 do not react as much as possible, and the three do not contaminate each other as much as possible, so as to achieve better measurement effect.

[0043] Optionally, since part of the salt bridge 3 in the second channel 2 is longer (has a larger surface area), in order to avoid bubbles being generated near this section of the salt bridge 3 , the adsorption element 56 may be specifically disposed adjacent to the portion of the salt bridge 3 exposed in the second channel 2 .

[0044] In one embodiment, the sample test card 9 may further include a first plate 4. The first plate 4 is provided with a groove 43 for filling the salt bridge 3, and the first electrode 41 and the second electrode 42 are arranged on a side surface of the first plate 4 provided with the groove 43. One end of the salt bridge 3 filled in the groove 43 is close to the first electrode 41, and the other end is close to the second electrode 42, so that the circuit is connected when the liquid is filled in the first channel 1 and the second channel 2.

[0045] Optionally, the sample test card 9 may further include a second plate 5. The second plate 5 is provided with a first through hole 51 and a second through hole 52. The second plate 5 is disposed on a side of the first plate 4 provided with the groove 43, and one end of the salt bridge 3 and the first electrode 41 are exposed to the first channel 1 through the first through hole 51, and the other end of the salt bridge 3 and the second electrode 42 are exposed to the second channel 2 through the second through hole 52.

[0046] Optionally, the second plate 5 covers at least the groove 43 area of ​​the first plate 4 to form a third channel for accommodating the salt bridge 3, one end of the third channel leads to the first channel 1, and the other end leads to the second channel 2. The salt bridge 3 can expand and block the third channel when in contact with the liquid to separate the first channel from the second channel.

[0047] Optionally, since the ions in the salt bridge 3 continue to migrate in a directional manner, the ion concentration near the salt bridge 3 varies greatly, and being too close to the salt bridge 3 is not conducive to the electrode measuring stable electrochemical parameters. Therefore, although the salt bridge 3 is close to the first electrode 41 and the second electrode 42, it still needs to maintain a certain distance from the two for buffering, so that the first electrode 41 and the second electrode 42 can be tested in an environment with relatively stable concentration.

[0048] Optionally, since a large amount of liquid is usually required in the second channel 2 to provide a stable test environment for testing the sample, the circuit structure set for detecting the sample in the first channel 1 will also be more complicated. Therefore, in order to leave sufficient space for the first electrode 41 and the circuit structure arranged in the first channel 1, the space in the second channel 2 is generally larger than the space in the first channel 1, and the distance between the salt bridge 3 and the second electrode 42 in the second channel 2 is also larger than the distance between the salt bridge 3 and the first electrode 41 in the first channel 1.

[0049] Optionally, the sample test card 9 may further include a first shell 6, and the first shell 6 and the first plate 4 cooperate to clamp the second plate 5 to form a first channel 1 at a position corresponding to the first through-hole 51 and a second channel 2 at a position corresponding to the second through-hole 52, thereby utilizing the first shell 6, the first plate 4, and the second plate 5 to form a first channel 1 and a second channel 2 connected by a third channel.

[0050] Optionally, a first recess 53 is provided on the inner wall of the isolation portion 55 on the side of the second through-hole 52 close to the first through-hole 51, and a portion of the salt bridge 3 can be exposed in the second channel 2 through the first recess 53. Since the solid salt bridge 3 itself has certain limitations in terms of conductivity, in order to minimize the impedance brought by the salt bridge 3, the length of the salt bridge 3 isolated between the first channel 1 and the second channel 2 can be minimized. After the first recess 53 as shown in the figure is provided, the second channel 2 can be closer to the first channel 1, and the portion of the salt bridge 3 directly exposed in the second channel 2 is also larger, which is conducive to reducing impedance and improving the accuracy of electrochemical parameter measurement.

[0051] Optionally, in order to ensure the sealing performance of the first channel 1 and the second channel 2, the second plate 5 can be made of a material with a certain elasticity and a certain deformation, such as rubber, so that the first plate 4, the second plate 5, and the first housing 6 can achieve interference fit, so as to prevent liquid leakage and achieve a better sealing effect of the first channel 1 and the second channel 2.

[0052] Optionally, since the second plate 5 can be deformed to a certain extent, when the distance between the first through-hole 51 and the second through-hole 52 is too close and the isolation part 55 between the two is too narrow, the isolation part 55 is easily deformed excessively due to the insufficient structural strength and the impact force of the liquid pressure and the liquid inlet, resulting in leakage of the first channel 1 and the second channel 2 near the isolation part 55. Therefore, while reducing the length of the salt bridge 3 between the two channels, it is also necessary to provide sufficient support for the first recessed part 53. For example, the contour shape of the first recessed part 53 is set to an arc shape, so that the liquid pressure on the material of the first recessed part 53 is reduced while the isolation part 55 of other parts has a sufficient width, thereby ensuring the stability of the structure.

[0053] The adsorption element 56 in this embodiment can be specifically referred to in Figures 5 to 8 , Figure 5 yes Figure 1 Schematic diagram of the assembly structure of the first plate and the second plate at another angle. Figure 6 yes Figure 5 Schematic diagram of the structure after the D area is enlarged. Figure 7 yes Figure 1 Schematic diagram of the cross-sectional structure of the sample test card from another angle. Figure 8 yes Figure 7 Schematic diagram of the structure after the B area is enlarged.

[0054] Due to the presence of the first recessed portion 53, the liquid filled in the second channel 2 may form bubbles when flowing through the first recessed portion 53, which may affect the electrical properties of the salt bridge 3. Therefore, the sample test card 9 may also be provided with an adsorption element 56 for the vicinity of the salt bridge 3 at the first recessed portion 53, so that the adsorption element 56 adsorbs the liquid in the second channel 2 onto the salt bridge 3 or near the salt bridge 3, ensuring that the liquid can reach the salt bridge 3 and guaranteeing the normal operation of the salt bridge 3. In addition, the presence of the adsorption element 56 can also squeeze out the bubbles that may appear near the salt bridge 3 as much as possible, thereby further ensuring that the liquid is in full contact with the salt bridge 3 and improving the accuracy of the test.

[0055] One end of the adsorption element 56 is close to the first recessed portion 53 , and the other end thereof extends in a direction away from the first through-hole 51 , so as to at least partially cover the salt bridge 3 exposed in the second channel 2 .

[0056] Optionally, the contour shape of one end of the adsorption element 56 close to the first recess 53 is complementary to the contour shape of the first recess 53, so that the end of the adsorption element 56 close to the first recess 53 can be spliced ​​and contacted with the first recess 53, so as to better fit with the salt bridge 3 arranged in the first recess 53 after absorbing water, and expel bubbles that may be generated in the first recess 53.

[0057] Optionally, the adsorption element 56 is made of a material that can be wetted when in contact with the reference solution passed into the second channel 2, such as one or more of the following materials: non-woven fabric, absorbent filter paper, absorbent cotton.

[0058] Optionally, the width of the salt bridge 3 is smaller than or equal to the opening size of the first recess 53 , so that the salt bridge 3 can be completely covered by the adsorption element 56 disposed near the first recess 53 in the width direction.

[0059] Optionally, the salt bridge 3 made of hydrogel material may swell after absorbing water, and the swollen salt bridge 3 can be slightly squeezed by the third channel to block the third channel, thereby preventing the mixing of the liquids on both sides of the salt bridge 3. However, when the amount of hydrogel in the salt bridge 3 is too much but the space in the third channel is too small, the swollen salt bridge 3 will be excessively squeezed, causing the salt bridge 3 to deform in a way that is difficult to effectively control, affecting the conduction effect of the circuit. Therefore, an arched space can be reserved on the surface of the second plate 5 that covers part of the surface of the salt bridge 3 and faces the salt bridge 3, so as to avoid the salt bridge 3 from being severely deformed by excessive squeezing after expansion, affecting its performance.

[0060] Optionally, considering that mechanical processing is inconvenient for machining structures that are too small and too precise, the volume of the arched space may be set slightly larger than the expanded volume of the salt bridge 3. In order to offset the error caused by mechanical precision, the amount of the salt bridge 3 filled in the third channel may be appropriately increased so that the salt bridge 3 can effectively block the third channel after expansion without causing serious deformation.

[0061] like Figure 6 and Figure 8 As shown, a first arched groove 54 is provided on the surface of the second plate 5 close to the salt bridge 3 at a position opposite to the groove 43 . After absorbing water, the salt bridge 3 expands toward the second plate 5 , thereby filling the first arched groove 54 .

[0062] Optionally, the depth of the first arched groove 54 relative to the surface of the second plate 5 facing the salt bridge 3 is related to the ability of the hydrogel in the salt bridge 3 to absorb water and swell and the volume of the hydrogel in the groove 43. The first arched groove 54 is configured to be just filled with the hydrogel after absorbing water, thereby ensuring the performance of the salt bridge 3 while avoiding the mutual mixing of the liquids in the first channel 1 and the second channel 2.

[0063] Optionally, the first arched groove 54 penetrates from one side of the first recessed portion 53 to the side of the isolation portion 55 close to the first through-port 51, so that the salt bridge 3 below the positive projection of the first arched groove 54 can protrude from the groove 43 after expansion, and block the first arched groove 54 so that the liquids in the first channel 1 and the second channel 2 are not mixed.

[0064] Optionally, in order to match the expansion characteristics of the salt bridge 3 and / or the adsorption element 56, similar to the first arched groove 54, a second arched groove 551 may be provided on the surface of the adsorption element 56 near the salt bridge 3. Figure 6 As shown, the adsorption element 56 can be arranged above the salt bridge 3 in a “bridge-like” manner.

[0065] Optionally, considering that mechanical processing is inconvenient for processing structures with too small size and too high precision, the volume of the second arched groove 551 will be set to be slightly larger than the sum of the volume of the adsorption element 56 expanded toward the salt bridge 3 and the volume of the salt bridge 3 expanded. In order to offset the error caused by mechanical precision, the amount of the salt bridge 3 filled in the third channel can be appropriately reduced, or the volume or thickness of the adsorption element 56 can be reduced without affecting the adsorption function, so that the salt bridge 3 will not be excessively squeezed by the adsorption element and severely deformed after expansion.

[0066] Optionally, the adsorption element 56 further has a support portion 552 , which is disposed on both sides of the second arched groove 551 and is used to fix the adsorption element 56 relative to the first plate 4 , thereby preventing the position of the adsorption element 56 from being changed due to the liquid in the second channel 2 .

[0067] Optionally, when the material of the adsorption element 56 is not easy to deform, the depths of the first arched groove 54 and the second arched groove 551 can be the same according to the properties of the salt bridge 3. When the adsorption element 56 also undergoes deformations such as expansion and / or collapse after absorbing water, the depth of the second arched groove 551 needs to be set in consideration of the properties of both the salt bridge 3 and the adsorption element 56. As a result, the salt bridge 3 and the adsorption element 56 are not necessarily in direct contact when dry, so as to avoid the salt bridge from being deteriorated due to ion migration after the sample test card 9 is produced, thereby improving the stability of the sample test card 9. When the sample test card 9 is used, after being wetted by liquid, the salt bridge 3 and the adsorption element 56 will each undergo different deformations, so that the distance between the two is reduced or they can be attached to each other, so as to avoid the situation where bubbles attached to the surface of the salt bridge affect conduction.

[0068] See also Fig. 9 , Fig. 9 yes Figure 1 Schematic diagram of the explosion structure of the sample test card at another angle. In order to achieve better measurement results, a certain amount of space needs to be designed in the first channel 1 and the second channel 2 of the test card to accommodate a sufficient amount of liquid, so a cavity 61 may also be provided on the first shell 6.

[0069] Among them, for the first channel 1 and the second channel 2, the cavity wall of the cavity 61 can have a first fluid groove 62 and a second fluid groove 63, the plate is located in the cavity 61, the first through hole 51 connects to a part of the first fluid groove 62, the second through hole 52 connects to a part of the second fluid groove 63, and the plate closes another part of the first fluid groove 62 and another part of the second fluid groove 63 to form the first channel 1 and the second channel 2 connected only by the salt bridge 3.

[0070] The above are only implementation methods of the present application, and are not intended to limit the patent scope of the present application. Any equivalent structure or equivalent process transformation made using the contents of the present application specification and drawings, or directly or indirectly applied in other related technical fields, are also included in the patent protection scope of the present application.

Claims

1. A sample test card, the sample test card is a test card for a blood gas analyzer, It is characterized in that include: A first channel, a second channel, a first electrode located in the first channel, and a second electrode located in the second channel; The first electrode is used to obtain electrochemical parameters of the sample in the first channel, and the second electrode is used to obtain electrochemical parameters of the reference solution in the second channel; a salt bridge, one end of the salt bridge being exposed in the first channel, and the other end of the salt bridge being exposed in the second channel; An adsorption element is disposed adjacent to the salt bridge.

2. The sample test card according to claim 1, It is characterized in that The adsorption element can be wetted by the liquid in the first channel and / or the second channel. When the adsorption element is in an unwetted state and / or when the salt bridge is in an unwetted state, the adsorption element and the salt bridge are spaced apart. When the adsorption element is in a wetted state and / or when the salt bridge is in a wetted state, the adsorption element and at least one of the salt bridge expand so that the two are in contact.

3. The sample test card according to claim 1, It is characterized in that The surface area of ​​the portion of the salt bridge exposed in the first channel is smaller than the surface area of ​​the portion of the salt bridge exposed in the second channel; or, The length of the portion of the salt bridge exposed in the first channel is shorter than the length of the portion of the salt bridge exposed in the second channel.

4. The sample test card according to claim 3, It is characterized in that The adsorption element is disposed adjacent to a portion of the salt bridge exposed to the second channel.

5. The sample test card according to claim 1, It is characterized in that include: The first plate has a groove formed therein, and the groove is used to fill the salt bridge. The first electrode and the second electrode are arranged on the first plate, and one end of the salt bridge filled in the groove is close to the first electrode, and the other end of the salt bridge is close to the second electrode.

6. The sample test card according to claim 5, It is characterized in that include: A second plate member is provided with a first through hole and a second through hole, the second plate member is arranged on a side of the first plate member provided with the groove, one end of the salt bridge and the first electrode are exposed through the first through hole, and the other end of the salt bridge and the second electrode are exposed through the second through hole; A first shell, wherein the first shell and the first plate cooperate to clamp the second plate to form the first channel corresponding to the first through-hole and the second channel corresponding to the second through-hole; The second plate at least covers the groove area of ​​the first plate to form a third channel for accommodating the salt bridge, one end of the third channel leads to the first channel, and the other end leads to the second channel. The salt bridge contacts the liquid and can expand and block the third channel to separate the first channel and the second channel.

7. The sample test card according to claim 6, It is characterized in that A first recessed portion is provided on the inner wall of the second through-hole close to the first through-hole, and the salt bridge portion is exposed to the second channel through the first recessed portion; One end of the adsorption element is close to the first recessed portion, and the other end extends in a direction away from the first through-hole, so as to at least partially cover the salt bridge exposed in the second channel.

8. The sample test card according to claim 7, It is characterized in that The contour shape of one end of the adsorption element close to the first recessed portion is complementary to the contour shape of the first recessed portion, and the end of the adsorption element close to the first recessed portion is in splicing contact with the first recessed portion.

9. The sample test card according to claim 7, It is characterized in that The width of the salt bridge is less than or equal to the opening size of the first recessed portion.

10. The sample test card according to any one of claims 1 to 9, It is characterized in that The adsorption element is one or more of the following materials: non-woven fabric, absorbent filter paper, absorbent cotton.

11. The sample test card according to any one of claims 1 to 9, It is characterized in that The first electrode includes a counter electrode and an ion electrode, the second electrode is a reference electrode, and the counter electrode, the ion electrode and the reference electrode form a three-electrode system for testing samples under the conduction effect of the salt bridge.

12. The sample test card according to claim 11, When the liquid in the measuring channel of the first channel is discharged, a small amount of liquid is used to form a conductive salt bridge and a liquid film of the first electrode, so that the three-electrode system circuit can remain conductive.

13. The sample test card according to claim 11, It is characterized in that The first electrode is not in direct contact with the salt bridge.

14. The sample test card according to any one of claims 5 to 9, It is characterized in that A coating for protecting the circuit structure in the first board is provided on a surface of the first board corresponding to the first channel and the second channel.

15. The sample test card according to any one of claims 1 to 9, It is characterized in that The electrolyte component of the salt bridge does not react with the liquid in the first channel and the second channel.

16. The sample test card according to any one of claims 1 to 9, It is characterized in that The adsorption element is made of a material that can be wetted when in contact with the reference solution passed into the second channel.

17. The sample test card according to any one of claims 7 to 9, It is characterized in that The width of the salt bridge is smaller than or equal to the opening size of the first recessed portion, so that the salt bridge can be completely covered by the adsorption element disposed near the first recessed portion in the width direction.

18. The sample test card according to any one of claims 1 to 9, It is characterized in that The salt bridge is not in direct contact with the adsorption element in the dry condition.

19. The sample test card according to any one of claims 6 to 9, It is characterized in that A first arched groove is provided on a surface of the second plate member on one side close to the salt bridge at a position opposite to the groove. After absorbing water, the salt bridge expands toward the second plate member, thereby filling the first arched groove.

20. The sample test card according to claim 19, It is characterized in that The salt bridge below the orthographic projection of the first arched groove protrudes from the groove after expansion, and blocks the first arched groove to prevent the liquids in the first channel and the second channel from mixing.

21. The sample test card according to any one of claims 1 to 9, It is characterized in that The adsorption element is arranged in a "bridge-like" manner above the salt bridge.

22. The sample test card according to any one of claims 12, 13, and 20, It is characterized in that The adsorption element is one or more of the following materials: non-woven fabric, absorbent filter paper, absorbent cotton.

23. The sample test card according to claim 11, It is characterized in that A measurement channel is provided in the first channel, and the counter electrode and the ion electrode are arranged along the measurement channel, wherein the counter electrode is close to the salt bridge, and the ion electrodes are located on both sides of the counter electrode.

24. The sample test card according to any one of claims 6 to 9, It is characterized in that A temperature measuring component is provided on the first plate to measure the temperature inside the sample test card; The temperature measuring component is a thermistor protruding toward one side of the first channel relative to the first plate; A second recessed portion is provided at a position of the second plate corresponding to the thermistor so as to avoid the thermistor; The thermistor is arranged at a position close to the edge of the second plate, so that the second recessed portion is recessed from the edge of the second plate toward the inside of the second plate.

25. The sample test card according to any one of claims 1 to 9, It is characterized in that The sample test card is also provided with a third electrode. The number of the third electrodes is several and they are respectively connected to the first electrode and the second electrode. The third electrodes obtain the electrochemical parameters measured by the first electrode and the second electrode.

26. The sample test card according to any one of claims 1 to 9, 12, 13, 20, 23, It is characterized in that The adsorption element is used to adsorb the liquid filled in the first channel and / or the second channel to the vicinity of the salt bridge so that the salt bridge is in contact with the liquid.

27. A sample test card, It is characterized in that include: A first channel, a second channel, a first electrode located in the first channel, and a second electrode located in the second channel; The first electrode is used to obtain electrochemical parameters of the sample in the first channel, and the second electrode is used to obtain electrochemical parameters of the reference solution in the second channel; a salt bridge, one end of the salt bridge being exposed in the first channel, and the other end of the salt bridge being exposed in the second channel; an adsorption element disposed adjacent to the salt bridge; in, The adsorption element can be wetted by the liquid in the first channel and / or the second channel. When the adsorption element is in an unwetted state and / or when the salt bridge is in an unwetted state, the adsorption element is spaced apart from the salt bridge. When the adsorption element is in a wetted state and / or when the salt bridge is in a wetted state, the adsorption element expands so that the adsorption element contacts the salt bridge.

28. The sample test card according to claim 27, It is characterized in that include: A first plate is provided with a groove, wherein the groove is used to fill the salt bridge, the first electrode and the second electrode are arranged on the first plate, one end of the salt bridge filled in the groove is close to the first electrode, and the other end of the salt bridge is close to the second electrode; A second plate member is provided with a first through hole and a second through hole, the second plate member is arranged on a side of the first plate member provided with the groove, one end of the salt bridge and the first electrode are exposed through the first through hole, and the other end of the salt bridge and the second electrode are exposed through the second through hole; A first shell, wherein the first shell and the first plate cooperate to clamp the second plate to form the first channel corresponding to the first through-hole and the second channel corresponding to the second through-hole; in, The second plate at least covers the groove area of ​​the first plate to form a third channel for accommodating the salt bridge, one end of the third channel leads to the first channel, and the other end leads to the second channel. The salt bridge contacts the liquid and can expand and block the third channel to separate the first channel and the second channel.

29. The sample test card according to claim 28, It is characterized in that A first recessed portion is provided on the inner wall of the second through-hole close to the first through-hole, and the salt bridge portion is exposed to the second channel through the first recessed portion; One end of the adsorption element is close to the first recessed portion, and the other end thereof extends in a direction away from the first through-hole to at least partially cover the salt bridge exposed in the second channel; The contour shape of one end of the adsorption element close to the first recessed portion is complementary to the contour shape of the first recessed portion, and the one end of the adsorption element close to the first recessed portion is spliced ​​and in contact with the first recessed portion.

30. The sample test card according to claim 28 or 29, It is characterized in that The adsorption element is one or more of the following materials: non-woven fabric, absorbent filter paper, absorbent cotton.

31. The sample test card according to claim 28 or 29, It is characterized in that The first electrode includes a counter electrode and an ion electrode, the second electrode is a reference electrode, and the counter electrode, the ion electrode and the reference electrode form a three-electrode system for testing a sample under the conduction effect of the salt bridge; When the liquid in the measuring channel of the first channel is discharged, a small amount of liquid is used to form a conductive salt bridge and a liquid film of the first electrode, so that the three-electrode system circuit can remain conductive.

32. The sample test card according to claim 28 or 29, It is characterized in that The second plate is made of an elastic compressible material so that the first plate, the second plate and the first shell can be interference fit; The adsorption element is used to adsorb the liquid filled in the first channel and / or the second channel to the vicinity of the salt bridge so that the salt bridge is in contact with the liquid.

33. A sample test card, It is characterized in that include: A first channel, a second channel, a first electrode located in the first channel, and a second electrode located in the second channel; The first electrode is used to obtain electrochemical parameters of the sample in the first channel, and the second electrode is used to obtain electrochemical parameters of the reference solution in the second channel; a salt bridge, one end of the salt bridge being exposed in the first channel, and the other end of the salt bridge being exposed in the second channel; an adsorption element disposed adjacent to the salt bridge; The adsorption element can be wetted by the liquid in the first channel and / or the second channel, when the adsorption element is in an unwetted state and / or when the salt bridge is in an unwetted state, the adsorption element and the salt bridge are spaced apart, and when the adsorption element is in a wetted state and / or when the salt bridge is in a wetted state, at least one of the adsorption element and the salt bridge expands so that the two are in contact; The first electrode includes a counter electrode and an ion electrode, the second electrode is a reference electrode, and the counter electrode, the ion electrode and the reference electrode form a three-electrode system for testing samples under the conduction effect of the salt bridge.

34. The sample test card according to claim 33, It is characterized in that When the liquid in the measuring channel of the first channel is discharged, a small amount of liquid is used to form a conductive salt bridge and a liquid film of the first electrode, so that the three-electrode system circuit can remain conductive.

35. The sample test card according to claim 33, It is characterized in that The adsorption element is one or more of the following materials: non-woven fabric, absorbent filter paper, absorbent cotton.

36. The sample test card according to any one of claims 33, 34, and 35, Features , The adsorption element is used to adsorb the liquid filled in the first channel and / or the second channel to the vicinity of the salt bridge so that the salt bridge is in contact with the liquid.

37. A sample test card, It is characterized in that include: A first channel, a second channel, a first electrode located in the first channel, and a second electrode located in the second channel; The first electrode is used to obtain electrochemical parameters of the sample in the first channel, and the second electrode is used to obtain electrochemical parameters of the reference solution in the second channel; a salt bridge, one end of the salt bridge being exposed in the first channel, and the other end of the salt bridge being exposed in the second channel; an adsorption element disposed adjacent to the salt bridge; The adsorption element can be wetted by the liquid in the first channel and / or the second channel. When the adsorption element is in an unwetted state and / or when the salt bridge is in an unwetted state, the adsorption element and the salt bridge are spaced apart from each other. When the adsorption element is in a wetted state and / or when the salt bridge is in a wetted state, the salt bridge expands so that the two are in contact.

38. The sample test card according to claim 37, It is characterized in that include: The first plate has a groove formed therein, and the groove is used to fill the salt bridge. The first electrode and the second electrode are arranged on the first plate, and one end of the salt bridge filled in the groove is close to the first electrode, and the other end of the salt bridge is close to the second electrode.

39. The sample test card according to claim 38, It is characterized in that include: A second plate member is provided with a first through hole and a second through hole, the second plate member is arranged on a side of the first plate member provided with the groove, one end of the salt bridge and the first electrode are exposed through the first through hole, and the other end of the salt bridge and the second electrode are exposed through the second through hole; A first shell, wherein the first shell and the first plate cooperate to clamp the second plate to form the first channel corresponding to the first through-hole and the second channel corresponding to the second through-hole; The second plate at least covers the groove area of ​​the first plate to form a third channel for accommodating the salt bridge, one end of the third channel leads to the first channel, and the other end leads to the second channel. The salt bridge contacts the liquid and can expand and block the third channel to separate the first channel and the second channel.

40. The sample test card according to claim 39, It is characterized in that A first recessed portion is provided on the inner wall of the second through-hole close to the first through-hole, and the salt bridge portion is exposed to the second channel through the first recessed portion; One end of the adsorption element is close to the first recessed portion, and the other end extends in a direction away from the first through-hole, so as to at least partially cover the salt bridge exposed in the second channel.

41. The sample test card according to claim 40, It is characterized in that The contour shape of one end of the adsorption element close to the first recessed portion is complementary to the contour shape of the first recessed portion, and the end of the adsorption element close to the first recessed portion is in splicing contact with the first recessed portion.

42. The sample test card according to claim 40, It is characterized in that The width of the salt bridge is less than or equal to the opening size of the first recessed portion.

43. A sample test card according to any one of claims 37 to 42, It is characterized in that The first electrode includes a counter electrode and an ion electrode, the second electrode is a reference electrode, and the counter electrode, the ion electrode and the reference electrode form a three-electrode system for testing a sample under the conduction effect of the salt bridge; When the liquid in the measuring channel of the first channel is discharged, a small amount of liquid is used to form a conductive salt bridge and a liquid film of the first electrode, so that the three-electrode system circuit can remain conductive.

44. A sample test card according to any one of claims 37 to 42, It is characterized in that The adsorption element is one or more of the following materials: non-woven fabric, absorbent filter paper, absorbent cotton.

45. A sample test card according to any one of claims 37 to 42, Features , The adsorption element is used to adsorb the liquid filled in the first channel and / or the second channel to the vicinity of the salt bridge so that the salt bridge is in contact with the liquid.

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