Immunohistochemical reaction system and method

Abstract

The present application discloses an immunohistochemical reaction system and method, which relates to the field of biomedical technology and is primarily intended to address the problem of poor immunohistochemical staining effects in existing immunohistochemical processes. The system comprises: a tissue sample incubation area, a robotic arm, a slide holder, and a controller. The tissue sample incubation area includes a multi-layer reaction tray, the reaction tray includes multiple slide heating zones, the slide heating zones are provided with heaters aligned with slide slots on the slide holder, and the reaction trays enter and exit the tissue sample incubation area via conveying devices provided on both sides of the tissue sample incubation area. The controller is configured to determine a target slide identifier of a target tissue slide and, in accordance with the target slide identifier, drive the robotic arm to place the slide holder carrying the target tissue slide into the target slide heating zone; and control the target heater corresponding to the target slide heating zone to heat the sample according to a preset heating threshold.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to an immunohistochemical reaction system and method. Background Art

[0002] Immunohistochemistry (IHC) is a practical biological experimental technique that uses the principle of specific binding of antigens and antibodies, and uses enzymes, proteins or chemical dyes to display the expression of macromolecular proteins or genes in vivo in situ on tissue sections or cell slices. It is widely used in the pathological diagnosis of various diseases such as tumors and inflammation. With the development of digital pathology technology, in order to better perform pathological diagnosis, automated immunohistochemical staining equipment has been put into operation to facilitate identification and analysis based on the tissue after the reaction. Among them, when performing immunohistochemical staining on tissue samples on glass slides, it is necessary to titrate the reagents, and then undergo heating and constant temperature incubation. At present, when incubating tissue samples on glass slides, it is necessary to place the glass slide in a fixed position to absorb the liquid in the reagent bottle and then titrate the reagent onto the glass slide, and then place each glass slide in the incubation area for reaction. Usually, a heater is installed to heat all the glass slides uniformly. However, placing slides one by one will generate a large number of robotic arm linkage operations, which greatly increases the operating cost of the equipment. In addition, a single heater in the incubation area cannot ensure that the heating temperature of the slides at various positions is consistent, which greatly reduces the immunohistochemical staining effect. Summary of the Invention

[0003] In view of this, the present application provides an immunohistochemical reaction system and method, the main purpose of which is to solve the problem of poor immunohistochemical staining effect in existing immunohistochemistry.

[0004] According to one aspect of the present application, an immunohistochemical reaction system is provided, comprising:

[0005] Tissue sample incubation area, robotic arm, slide holder, controller,

[0006] The tissue sample incubation area includes a multi-layer reaction tray, which includes multiple slide heating zones. The slide heating zones are provided with heaters aligned with the slide slots on the slide holders. The reaction tray enters and exits the tissue sample incubation area via conveying devices provided on both sides of the tissue sample incubation area.

[0007] The robotic arm is used to clamp the slide holder and place it on the slide heating zone on the reaction tray extending out of the tissue sample incubation zone to perform immunohistochemical staining;

[0008] The slide holder is used to load a plurality of tissue slides;

[0009] The controller is used to determine the target slide identifier of the target tissue slide, and drive the robotic arm to place the slide clamp carrying the target tissue slide in the target slide heating zone according to the target slide identifier; and control the target heater corresponding to the target slide heating zone to heat according to a preset heating threshold.

[0010] Furthermore, the tissue sample incubation area further includes a motor electrically connected to the conveying device and configured to drive the conveying device in accordance with an extension instruction or an entry instruction from the controller; the robotic arm is provided with a clamping claw assembly; the slide holder includes a slide holder body and a slide holder chuck, the slide holder chuck being provided on one side of the slide holder body, and having slots on both sides of the slide holder chuck capable of engaging with the clamping claws for clamping;

[0011] The controller is further configured to query the slide heating zones in the reaction tray that are in an idle state, and determine a target slide heating zone according to the idle state;

[0012] The controller is further configured to determine a conveying path for the robotic arm to grip the target glass slide holder, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target glass slide heating zone, drive the robotic arm according to the conveying path to grip the glass slide holder and place it in the target glass slide heating zone. The conveying path is a route determined based on the placement position of the target glass slide heating zone and the gripping position of the target glass slide holder.

[0013] Furthermore, the system also includes a dripping device for sucking and dripping out medicine; the tissue sample incubation area also includes a medicine refrigeration area and a medicine storage area arranged on the top layer of the reaction tray; the medicine refrigeration area is used to provide cold air to the reaction tray; the medicine storage area includes a plurality of medicine storage slots for storing medicine bottles;

[0014] The controller is further configured to determine the target medicine and target dripping amount according to the target slide identification, and drive the dripping device to draw the medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating zone.

[0015] Furthermore, the medicine storage tank includes a first medicine storage tank and a second medicine storage tank, the first medicine storage tank is arranged in a honeycomb shape, and the second medicine storage tank is arranged in parallel according to preset rows and preset columns;

[0016] The controller is further configured to determine the suction position of the dripping device according to the target medicine, and determine the dripping position of the dripping device in combination with the target slide heating zone; and generate a driving route of the dripping device according to the suction position and the dripping position.

[0017] Furthermore, partitions are provided on both sides of the slide heating zone for stabilizing the slide clamp, and the height of the partitions is greater than the thickness of the slide clamp.

[0018] Furthermore, a limit stopper is provided on the side of the reaction tray, and a frame baffle aligned with the limit stopper is provided on the inner side of the shell of the tissue sample incubation area for limiting the extended length of the reaction tray.

[0019] Furthermore, a drainage hole is provided on the bottom surface of the reaction tray, and a drip groove is slidably connected to the drainage hole on the bottom surface. The drip groove has a notch, and a drainage pipe mouth is provided at the drainage hole. The drainage pipe mouth extends toward the drip groove and is provided at the far end of the notch.

[0020] According to another aspect of the present application, a reaction method for immunohistochemical staining is provided, comprising:

[0021] When a target slide holder is loaded with a plurality of tissue slides, a target slide identifier of the target slide holder is obtained by a controller, and a target slide heating zone in a tissue sample incubation zone is determined based on the target slide identifier, wherein the tissue sample incubation zone includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating zones, each of the slide heating zones is provided with a heater aligned with a slide slot on the slide holder, and the reaction tray enters and exits the tissue sample incubation zone via a conveying device provided on both sides of the tissue sample incubation zone;

[0022] The controller drives the robotic arm according to the target slide identifier to place the slide holder carrying the target tissue slide in the target slide heating zone, and controls the target heater on the target slide heating zone to heat according to a preset heating threshold.

[0023] By means of the above technical solution, the technical solution provided by the embodiment of the present application has at least the following advantages:

[0024] The present application provides an immunohistochemical reaction system and method. Compared with the prior art, the reaction system in the embodiment of the present application includes: a tissue sample incubation area, a robotic arm, a slide clamp, and a controller. The tissue sample incubation area includes a multi-layer reaction tray, the reaction tray includes multiple slide heating areas, the slide heating areas are provided with heaters aligned with the slide grooves on the slide clamp, the reaction tray enters and exits the tissue sample incubation area through a conveying device provided on both sides of the tissue sample incubation area; the robotic arm is used to clamp the slide clamp and place it on the slide heating area on the reaction tray extending from the tissue sample incubation area to perform immunohistochemistry. Immunohistochemical staining; the slide holder is used to load multiple tissue slides; the controller is used to determine the target slide identifier of the target tissue slide, and drive the robotic arm to place the slide holder carrying the target tissue slide in the target slide heating zone according to the target slide identifier; according to the preset heating threshold, the target heater corresponding to the target slide heating zone is controlled to heat, thereby greatly increasing the holding capacity of the slides in the tissue sample incubation zone and reducing the transmission effect of the robotic arm in a limited space. By configuring the heating zone in each slide heating zone, the tissue sample is accurately heated in the tissue sample incubation zone, thereby improving the effectiveness of immunohistochemical staining.

[0025] Typically, a single heater is installed to uniformly heat all slides. However, placing slides one by one requires numerous robotic arm manipulations, significantly increasing equipment operating costs. Furthermore, a single heater in the incubation area cannot ensure consistent heating temperatures for slides at all locations, significantly reducing immunohistochemical staining results.

[0026] The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification. In order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present application. The same reference symbols are used throughout the drawings to represent the same components. In the drawings:

[0028] Figure 1 Shown is a structural block diagram of an immunohistochemical reaction system provided in an embodiment of the present application;

[0029] Figure 2 A schematic diagram of the structure of a tissue sample incubation area provided in an embodiment of the present application is shown;

[0030] Figure 3 A schematic diagram of the structure of a transmission motor provided in an embodiment of the present application is shown;

[0031] Figure 4 A schematic structural diagram of a glass slide holder provided in an embodiment of the present application is shown;

[0032] Figure 5 A schematic diagram of the structure of a clamping claw and a glass slide clamping member provided in an embodiment of the present application is shown;

[0033] Figure 6 A schematic structural diagram of a dripping device provided in an embodiment of the present application is shown;

[0034] Figure 7 A schematic diagram of the structure of a medicine refrigeration area and a medicine storage area provided in an embodiment of the present application is shown;

[0035] Figure 8 A schematic diagram of a heating table structure provided in an embodiment of the present application is shown;

[0036] Figure 9 A schematic diagram of the structure of a limit block provided in an embodiment of the present application is shown;

[0037] Figure 10 A schematic diagram of the structure of a drip tank provided in an embodiment of the present application is shown;

[0038] Figure 11 A schematic diagram of an immunohistochemistry reaction process provided in an embodiment of the present application is shown;

[0039] Among them, there are tissue sample incubation area-1, robotic arm-2, slide clamp-3, controller-4, reaction tray-11, slide heating area-111, heater-112, conveying device-12, slide slot-31, motor-13, clamp-5, slide rack-32, slide rack chuck-33, card slot-34, dripping device-6, drug cooling area-7, drug storage area-8, drug storage tank-81, first drug storage tank-811, second drug storage tank-812, heating table-113, partition-114, limit baffle-14, rack baffle-15, drainage hole-16, dripping tank-17, drainage nozzle-161, and push baffle-18. DETAILED DESCRIPTION

[0040] Exemplary embodiments of the present disclosure will be described in more detail below with reference to the accompanying drawings. Although exemplary embodiments of the present disclosure are shown in the accompanying drawings, it should be understood that the present disclosure can be implemented in various forms and should not be limited by the embodiments set forth herein. Rather, these embodiments are provided to enable a more thorough understanding of the present disclosure and to fully convey the scope of the present disclosure to those skilled in the art.

[0041] The present invention provides an immunohistochemical reaction system. Figure 1 The system structure diagram shown in the figure includes: tissue sample incubation area 1, robotic arm 2, slide holder 3, controller 4,

[0042] Among them, such as Figure 2 As shown, the tissue sample incubation area 1 includes a multi-layer reaction tray 11, which includes multiple slide heating areas 111. The slide heating areas 111 are provided with heaters 112 aligned with the slide slots 31 on the slide holder 3. The reaction tray 11 enters and exits the tissue sample incubation area via conveying devices 12 provided on both sides of the tissue sample incubation area.

[0043] The robotic arm is used to clamp the glass slide holder 3 and place it on the glass slide heating zone 111 on the reaction tray 11 extending out of the tissue sample incubation zone 1 to perform immunohistochemical staining;

[0044] The slide holder 3 is used to load multiple tissue slides;

[0045] The controller 4 is used to determine the target slide identifier of the target tissue slide, and drive the robotic arm 2 to place the slide clamp 3 carrying the target tissue slide in the target slide heating zone 111 according to the target slide identifier; and control the target heater 112 corresponding to the target slide heating zone 111 to heat according to a preset heating threshold.

[0046] In the embodiment of the present application, in the medical field, immunohistochemical cell tissues, including but not limited to non-pathological tissues or pathological tissues in human organs, are sampled through glass slides as tissue samples. At this time, the glass slide groove 31 in the glass slide holder 3 can clamp the glass slide. A glass slide holder 3 includes multiple glass slide grooves 31, and correspondingly, multiple glass slides can be clamped. When it is necessary to heat the tissue sample, the slide is clamped and moved in the system by the robot arm 2. The tissue sample incubation area 1 includes multiple layers of reaction trays 11, which are arranged one by one in the tissue sample incubation area 1 according to the structure of each layer. Figure 2As shown, the reaction tray 11 includes multiple slide heating zones 111. Each slide heating zone 111 is provided with a heater 112 aligned with the slide slot 31 on the slide holder 3. When a slide holder 3 is directly placed on the slide heating zone 111, the aligned heating zone can directly heat the slide in the slide slot 31. The heater 112 is provided on a bridge-shaped table at the bottom of the reaction tray 11. The bridge-shaped table and the bottom form a cavity for storing dripping reagents. At the same time, a drainage hole 16 is provided at a corner position of the reaction tray 11. Figure 2 As shown, the slide heating area 111 in each layer of the reaction tray 11 is arranged in the longitudinal direction, so that the reaction tray 11 can heat multiple slides within the maximum space limit. Figure 2 As shown, the reaction tray 11 is further extended out of the tissue sample incubation area 1 by the conveying devices 12 arranged on both sides of the tissue sample incubation area 1, so that the slide holder 3 can be placed on or removed from the reaction tray 11 after extending out of the tissue sample incubation area 1.

[0047] It should be noted that the reaction system in the embodiment of the present application is triggered by a command from the controller 4. In this case, the controller 4 can be a terminal processor or a server device, and communicates data with the robotic arm 2, motor 13 and other devices via a communication line. This embodiment of the present application is not specifically limited. Among them, the user can transmit the target slide identification of the tissue slide on the slide holder 3 to the controller 4 by typing or taking a photo. A camera can also be configured to take a photo of the tissue slide and generate a corresponding target slide identification for the controller 4 to query and determine. This embodiment of the present application is not specifically limited. Furthermore, the controller 4 can determine the specific position of the slide heating zone 111 according to the target slide identification, that is, determine the target slide heating zone 111 to be placed. At this time, since the tissue sample incubation area 1 includes multiple layers of reaction trays 11, each layer of reaction tray 11 includes multiple slide heating zones 111. When determining the target slide heating zone 111, the idle slide heating zones 111 can be queried one by one. This embodiment of the present application is not specifically limited. After determining the target slide heating zone 111, the controller 4 sends a control instruction to the robot arm 2 to control the continuing arm to move the held target slide clamp 3 to the target slide heating zone 111 for placement, and then control the target heater 112 on the target slide heating zone 111 to heat according to the preset heating threshold. The preset heating threshold includes the heating time and the heating temperature. For example, heating is performed at ±36.5 degrees for 1 day to simulate human body temperature. This embodiment of the present application does not make specific limitations.

[0048] In addition, in the embodiment of the present application, since the reaction trays 11 are arranged layer by layer, in order to enable the reaction trays 11 to automatically and flexibly extend and retract to prevent the slices from drying, the reaction trays 11 enter and exit the tissue sample incubation area 1 through the conveying devices 12 arranged on both sides of the tissue sample incubation area 1, such as Figure 2 As shown, the conveyor device 12 includes wheels and a conveyor belt wrapped around the two wheels. A motor 13 drives the rear wheels to rotate, which in turn drives the front wheels via the conveyor belt. The front wheels are connected to the reaction trays 11 through a transmission, thereby driving each reaction tray 11 to extend or retract. The controller 4 can control the time when the motor 13 stops rotating to control the time the reaction tray 11 is extended or retracted, which is not specifically limited in this embodiment of the present application.

[0049] In another embodiment of the present application, for further definition and explanation, the tissue sample incubation area 1 further includes a motor 13, such as Figure 3 As shown, it is electrically connected to the conveying device 12 and is used to drive the conveying device 12 according to the extension command or the entry command of the controller 4. The robotic arm 2 is provided with a clamping claw 5 assembly; the slide clamping member 3 includes a slide frame 32 and a slide frame chuck 33, the slide frame chuck 33 is provided on one side of the slide frame 32, and the slide frame chuck 33 has a clamping groove 34 on both sides, and the clamping groove 34 can be engaged with the clamping claw 5 for clamping;

[0050] The controller 4 is further configured to query the slide heating zone 111 in the reaction tray 11 that is in an idle state, and determine a target slide heating zone 111 according to the idle state;

[0051] The controller 4 is further configured to determine a conveying path for the robotic arm 2 to grip the target slide holder 3, and after driving the motor 13 to drive the conveying device 12 to extend the reaction tray 11 corresponding to the target slide heating zone 111, drive the robotic arm 2 according to the conveying path to grip the slide holder 3 and place it in the target slide heating zone 111.

[0052] In order to accurately drive the reaction tray 11 to extend and retract through the controller 4, the tissue sample incubation area 1 further includes a motor 13, which is arranged on the back plate of each layer of the reaction tray 11. Figure 3As shown, it is electrically connected to the conveying device 12 and is used to drive the conveying device 12 according to the extension instruction or the entry instruction of the controller 4. When the controller 4 determines the target slide heating zone 111 and the corresponding reaction tray 11, it sends a control instruction to the corresponding motor 13 to drive the extension or retraction. The embodiment of the present application does not make specific limitations. At the same time, in order to enable the robot arm 2 to stably hold the slide clamp 3 so that the robot arm 2 can flexibly clamp it, a clamping claw 5 component is provided on the robot arm 2. At the same time, as shown Figure 4 As shown, in order to ensure that the glass slide holder 3 can be stably held by the clamping claw 5, the glass slide holder 3 includes a glass slide holder body 32 and a glass slide holder chuck 33. The glass slide holder chuck 33 is arranged on one side of the glass slide holder body 32. Card grooves 34 are provided on both sides of the glass slide holder chuck 33 so that the card grooves 34 and the clamping claw 5 can be engaged and clamped. At the same time, the glass slide holder body 32 also includes an elastic clip 35, which is made of elastic metal. One end is fixed to the glass slide holder body 32, and the other end holds the glass slide.

[0053] It should be noted that in order to ensure efficient use of the slide heating zones 111 in each layer of the reaction tray 11, the controller 4 may first query the idle slide heating zones in the reaction tray 11 to determine the target slide heating zone 111 from the idle slide heating zones 111. Specifically, the idle state may be determined based on the heating signal of the heater 112. To screen all idle slide heating zones 111, the reaction trays 11 may be sorted from top to bottom, and then an idle slide heating zone 111 may be randomly selected from the topmost reaction tray 11. Alternatively, all slide heating zones 111 may be numbered and the idle slide heating zones 111 may be sorted sequentially according to the numbers. This is not specifically limited in the present embodiment. After determining the target slide heating zone 111, in order to better control the operation of the robot arm 2 in the system environment and improve the movement efficiency, the controller 4 determines a transmission path according to the target reaction tray 11 and the target slide heating zone 111, so that the robot arm 2 can clamp the slide clamp 3 and move to the target slide heating zone 111 for placement. The transmission path is a route determined based on the placement position of the target slide heating zone 111 and the clamping position of the target slide clamp 3. The controller 4 determines the placement position of the target slide heating zone 111 and the clamping position of the target slide clamp 3 (for example, the position of the slide clamp 3 after repair in the repair instrument) by loading the movement space coordinates of the robot arm 2. Then, the placement position and the clamping position are optimized to obtain the transmission path. The embodiment of the present application does not specifically limit the path optimization method.

[0054] In the embodiment of the present application, the execution code for generating the transmission path in the controller 4 can be set based on the coordinates provided in the robot arm 2 and the pre-written script code, and the embodiment of the present application does not make any specific limitations.

[0055] In a specific embodiment, the slot 34 and the jaw 5 are designed to fit together. The slot 34 includes at least two groove surfaces, and the groove surfaces form a first angle. In contrast, the jaw 5 includes a first jaw side 51 and a second jaw side 52. Each jaw side is provided with a plurality of raised surfaces to form a claw body 53. The raised surfaces form a second angle. At this time, the first angle is complementary to the second angle, so that the slot 34 and the jaw 5 are fit together and clamped by the first angle and the second angle. Figure 5 shown.

[0056] In another embodiment of the present application, in order to further define and illustrate, Figure 6 As shown, the system further includes a dripping device 6 for sucking and dripping out medicine; the tissue sample incubation area 1 further includes a medicine cooling area 7 and a medicine storage area 88 provided on the top layer of the reaction tray 11; the medicine cooling area 7 is used to provide cold air to the reaction tray 11; the medicine storage area 8 includes a plurality of medicine storage slots 81 for storing medicine bottles;

[0057] The controller 4 is further configured to determine the target medicine and target dripping amount according to the target slide identification, and drive the dripping device 6 to draw the medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating zone 111 .

[0058] In order to achieve efficient use of the tissue sample incubation area 1 for immunohistochemical staining, the system also includes a dripping device 6 for sucking and dripping the drug. At the same time, in order to achieve efficient dripping, the drug cooling area 7 and the drug storage area 8 on the top layer of the tissue sample incubation area 1 are as follows: Figure 7 As shown, at this time, the drug refrigeration zone 7 is used to provide cold air to the reaction tray 11, and refrigerant can be used for refrigeration, which is not specifically limited in the embodiment of the present application. At the same time. The drug storage area 88 includes a plurality of drug storage slots 81 for storing drug bottles. Different drugs can be placed in different drug bottles. This is not specifically limited in the embodiment of the present application. At this time, in order to make the drug titration accurate and effective, the controller 4 first determines the target drug and the corresponding target dripping amount according to the target slide identification, and then drives the dripping device 6 to draw the drug from the target drug bottle, and then drips the drug onto the target tissue slide on the target slide heating zone 111. Among them, since the slide identification is information for the identity of the standard tissue sample, the controller 4 can pre-store drugs and dripping amounts that match different slide identifications for query and determination, which is not specifically limited in the embodiment of the present application.

[0059] In another embodiment of the present application, for further definition and explanation, the medicine storage tank 81 includes a first medicine storage tank 811 and a second medicine storage tank 812, wherein the first medicine storage tank 811 is arranged in a honeycomb shape, and the second medicine storage tank 812 is arranged in parallel according to preset rows and preset columns;

[0060] The controller 4 is further configured to determine the suction position of the dripping device 6 according to the target medicine, and determine the dripping position of the dripping device 6 in combination with the target slide heating zone 111; and generate a driving route of the dripping device 6 according to the suction position and the dripping position.

[0061] In order to place more reagents in the tissue sample incubation area 1, the reagent storage tank 81 includes a first reagent storage tank 811 and a second reagent storage tank 812. The first reagent storage tank 811 and the second reagent storage tank 812 respectively store reagent bottles with different reagent volumes. Figure 7 As shown, the first medicine storage slots 811 are arranged in a honeycomb shape, and the second medicine storage slots 812 are arranged in parallel according to preset rows (such as 2 rows) and preset columns (such as 8 columns) to distinguish the storage of medicines of different doses, which is not specifically limited in this embodiment of the present application.

[0062] Since the storage space for the medicine bottles is increased, the storage capacity of the medicine bottles is increased. In order to enable the dripping device 6 to drip more efficiently and accurately, the controller 4 determines the target medicine and the target dripping amount according to the target slide identification. Specifically, the corresponding dose mapping table can be pre-written in the controller 4, and the correspondence between different slide identifications and different medicines and different dripping amounts can be pre-stored so that the controller 4 can accurately take medicine for storing the medicines and dripping amounts required for the reaction of different slide identifications. This embodiment of the application does not make specific limitations. In addition, when controlling the dripping device 6, the controller 4 can drive the dripping device 6 to drip the target medicine from the target medicine bottle, and drive the dripping device 6 to drip the medicine to the target tissue slide on the target slide heating zone 111, and then send a heating instruction to the heating zone of the target slide heating zone 111. This embodiment of the application does not make specific limitations.

[0063] It should be noted that the dripping device 6 in the embodiment of the present application includes a needle, an air tube, and an air pump. After the air pump is activated, the liquid is sucked from the needle through the air tube and dripped out. In addition, the dripping device 6 can be set on a bracket with a conveyor belt to achieve control such as upward and downward movement and extension, which is not specifically limited in the embodiment of the present application.

[0064] In another embodiment of the present application, for further definition and explanation, partitions 114 are provided on both sides of the slide heating zone 111 for stabilizing the slide clamp 3 , and the height of the partitions 114 is greater than the thickness of the slide clamp 3 .

[0065] In order to ensure that the slide holder 3 can be stably placed in the slide heating area 111 of the reaction tray 11, the slide heating area 111 includes a heating table 113. The heater 112 is set on this bridge-shaped heating table 113. The heating table 113 can be made of heat-conducting metal. At this time, partitions 114 are set on both sides of the heating table 113. Figure 8 As shown, it is used to stabilize the slide holder 3. At this time, the height of the partition 114 is greater than the thickness of the slide holder 3, which is not specifically limited in the embodiment of the present application.

[0066] In another embodiment of the present application, for further definition and explanation, a limit stopper 14 is provided on the side of the reaction tray 11, and a frame baffle 15 is provided on the inner side of the shell of the tissue sample incubation area 1 to be aligned with the limit stopper 14, for limiting the extension length of the reaction tray 11.

[0067] In order to ensure that the reaction tray 11 is sufficiently safe during the extension and contraction process, thereby ensuring the effective reaction of the tissue sample, a limit stopper 14 is fixedly provided on the side of the reaction tray 11. Figure 9 As shown, a frame baffle 15 is correspondingly provided on the inner side of the shell of the tissue sample incubation area 1, aligned with the limit block 14, to limit the extension length of the reaction tray 11. The frame baffle 15 is formed by providing track holes in each side frame layer of the tissue sample incubation area 1. The limit block 14 moves within the track holes and limits the position. The limit block 14 may include a rear limit block 141 and a front limit block 142 to limit the position of the reaction tray 11 during its extension and retraction. This embodiment of the present application does not specifically limit this.

[0068] In another embodiment of the present application, for further limitation and explanation, a drainage hole 16 is provided on the bottom surface of the reaction tray 11, and a drip groove 17 is slidably connected to the drainage hole 16 on the bottom surface. The drip groove 17 has a notch, and a drainage pipe mouth 161 is provided at the drainage hole 16. The drainage pipe mouth 161 extends toward the drip groove 17 and is provided at the far end of the notch.

[0069] Since the reagents titrated on the glass slide may flow into the reaction tray 11 when the tissue sample is reacting, in order to avoid excessive humidity in the tissue sample incubation area 1 and affect the reaction effect, each layer of the reaction tray 11 is provided with a drainage hole 16. Figure 10As shown, the drainage hole 16 is preferably opened at the edge of the reaction tray 11, and the reaction tray 11 is made so that the horizontal surface at the drainage hole 16 is the lowest, and the residual liquid flows out of the drainage hole 16. A drip groove 17 is slidably provided on the bottom surface of the reaction tray 11 in alignment with the drainage hole 16. The drip groove 17 is provided at the bottom of the reaction tray 11 via a guide rail. At this time, a drainage nozzle 161 is provided at the drainage hole 16. The drainage nozzle 161 extends toward the drip groove 17 and is provided at a distance from the distal end of the nozzle. That is, when the reaction tray 11 is extended, the drainage nozzle 161 pushes one side of the drip groove 17 to slide out. After the reaction tray 11 is extended, the drainage nozzle 161 still drains liquid into the drip groove 17. This embodiment of the present application does not specifically limit this. At the same time, a push baffle 18 is fixedly connected to the bottom surface of the reaction tray 11 at a position corresponding to one side of the dripping groove 17, which is used to push the dripping groove 17 back to the initial position after the reaction tray 11 is retracted into the tissue sample incubation area 1. At this time, the initial position is the position where the dripping groove 17 is aligned with the outer side of the reaction tray 11. The length of the dripping groove 17 is preferably half the width of the reaction tray 11, which is not specifically limited in the embodiment of the present application.

[0070] In addition, since the drip groove 17 has a notch at the beginning, the residual liquid dripping from the upper reaction tray 11 will drip into the next reaction tray 11 along the notch of the drip groove 17, and finally drip into the bottom tray. At this time, the drainage pipe port 161 of the bottom tray can be directly connected to the waste liquid bucket, which is not specifically limited in the embodiment of the present application.

[0071] In addition, it should be noted that the materials not specifically described in the embodiments of the present application are all metals, without any specific limitation.

[0072] The present application provides an immunohistochemical reaction system that greatly increases the capacity of slides in the tissue sample incubation area and reduces the transmission effect of the robotic arm in a limited space. By configuring a heating zone in each slide heating zone, the tissue sample is accurately heated in the tissue sample incubation area, thereby improving the effectiveness of immunohistochemical staining.

[0073] Furthermore, as a response to the above Figure 1 The embodiment of the present application provides a method for immunohistochemical staining. Figure 3 As shown, the method includes:

[0074] 101. After a target slide holder is loaded with a plurality of tissue slides, a controller is used to obtain a target slide identifier of the target slide holder, and a target slide heating zone in a tissue sample incubation area is determined based on the target slide identifier, wherein the tissue sample incubation area includes a multi-layer reaction tray, the reaction tray including a plurality of slide heating zones, each of the slide heating zones being provided with heaters aligned with slide slots on the slide holder, and the reaction tray entering and exiting the tissue sample incubation area via conveying devices provided on both sides of the tissue sample incubation area;

[0075] 102. Drive the robotic arm by the controller according to the target slide identifier to place the slide holder carrying the target tissue slide in the target slide heating zone, and control the target heater on the target slide heating zone to heat according to a preset heating threshold.

[0076] In an embodiment of the present application, a user can load a slide holder holding multiple tissue slides into the hopper. A controller can use a photoelectric switch in the hopper to determine whether loading is complete, which serves as a trigger for the target slide holder to load the tissue slide. The controller further obtains a target slide identifier from the target slide holder and determines a target slide heating zone in the tissue sample incubation area based on the target slide identifier. Furthermore, the controller sends a control instruction to drive the robotic arm to place the slide holder carrying the target tissue slide into the target slide heating zone. Furthermore, the controller controls the target heater on the target slide heating zone to heat the target slide heating zone using a heating instruction that carries a preset heating threshold, thereby achieving immunohistochemical staining of the tissue sample.

[0077] In another embodiment of the present application, for further definition and explanation, the tissue sample incubation area further includes a motor electrically connected to the conveying device, for driving the conveying device according to the extension instruction or the entry instruction of the controller;

[0078] querying, by the controller, a slide heating zone in an idle state in the reaction tray, and determining a target slide heating zone according to the idle state;

[0079] The controller determines a conveying path for the robotic arm to clamp the target glass slide holder, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target glass slide heating zone, the robotic arm is driven according to the conveying path to clamp the glass slide holder and place it in the target glass slide heating zone. The conveying path is a route determined based on the placement position of the target glass slide heating zone and the clamping position of the target glass slide holder.

[0080] In another embodiment of the present application, for further definition and explanation, the tissue sample incubation area further includes a drug refrigeration area and a drug storage area disposed on a top layer of the reaction tray; the drug refrigeration area is used to provide cold air to the reaction tray; the drug storage area includes a plurality of drug storage slots for storing drug bottles; the drug storage slots include a first drug storage slot and a second drug storage slot, the first drug storage slots being arranged in a honeycomb pattern, and the second drug storage slots being arranged in parallel according to preset rows and preset columns;

[0081] The controller determines a target dose and a target dripping amount according to the target slide identifier, determines a suction position of a dripping device for sucking and dripping the dose according to the target dose, and determines a dripping position of the dripping device in combination with the target slide heating zone;

[0082] After the controller generates a driving route for the dripping device according to the suction position and the dripping position, the dripping device is driven to absorb medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating area.

[0083] The present application provides a reaction method for immunohistochemical staining, which greatly increases the capacity of slides in the tissue sample incubation area and reduces the transmission effect of the robotic arm in a limited space. By configuring a heating zone in each slide heating zone, the tissue sample is accurately heated in the tissue sample incubation area, thereby improving the effectiveness of immunohistochemical staining.

[0084] Obviously, those skilled in the art should understand that the modules or steps of the present application described above can be implemented using a general-purpose computing device, they can be concentrated on a single computing device, or distributed on a network composed of multiple computing devices. Alternatively, they can be implemented using program code executable by the computing device, so that they can be stored in a storage device and executed by the computing device. In some cases, the steps shown or described can be performed in a different order than herein, or they can be made into separate integrated circuit modules, or multiple modules or steps can be made into a single integrated circuit module for implementation. Thus, the present application is not limited to any specific combination of hardware and software.

[0085] The above description is merely a preferred embodiment of the present application and is not intended to limit the present application. Persons skilled in the art will readily appreciate that various modifications and variations are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present application shall be included within the scope of protection of the present application.

Claims

1. An immunohistochemical reaction system, characterized in that: include: Tissue sample incubation area, robotic arm, slide holder, controller, The tissue sample incubation area includes a multi-layer reaction tray, which includes multiple slide heating zones. The slide heating zones are provided with heaters aligned with the slide slots on the slide holders. The reaction tray enters and exits the tissue sample incubation area via conveying devices provided on both sides of the tissue sample incubation area. The robotic arm is used to clamp the slide holder and place it on the slide heating zone on the reaction tray extending out of the tissue sample incubation zone to perform immunohistochemical staining; The slide holder is used to load a plurality of tissue slides; The controller is configured to determine a target slide identifier of the target tissue slide, and drive the robotic arm to place the slide holder carrying the target tissue slide in the target slide heating zone according to the target slide identifier; Controlling the target heater corresponding to the target slide heating zone to heat according to a preset heating threshold; The tissue sample incubation area further includes a motor electrically connected to the conveying device and configured to drive the conveying device in accordance with an extension command or an entry command from the controller; the robotic arm is provided with a clamping claw assembly; the slide holder includes a slide holder body and a slide holder chuck, the slide holder chuck being provided on one side of the slide holder body, and having slots on both sides of the slide holder chuck capable of engaging with the clamping claws for clamping; The controller is further configured to query the slide heating zones in the reaction tray that are in an idle state, and determine a target slide heating zone according to the idle state; The controller is further configured to determine a conveying path for the robotic arm to grip the target glass slide holder, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target glass slide heating zone, drive the robotic arm according to the conveying path to grip the glass slide holder and place it in the target glass slide heating zone. The conveying path is a route determined based on the placement position of the target glass slide heating zone and the gripping position of the target glass slide holder.

2. The system according to claim 1, wherein: The system also includes a dripping device for sucking and dripping out the medicine; the tissue sample incubation area also includes a medicine cooling area and a medicine storage area arranged on the top layer of the reaction tray; the medicine cooling area is used to provide cold air to the reaction tray; the medicine storage area includes a plurality of medicine storage slots for storing medicine bottles; The controller is further configured to determine the target medicine and target dripping amount according to the target slide identification, and drive the dripping device to draw the medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating zone.

3. The system according to claim 2, characterized in that The medicine storage tank includes a first medicine storage tank and a second medicine storage tank, the first medicine storage tank is arranged in a honeycomb shape, and the second medicine storage tank is arranged in parallel according to preset rows and preset columns; The controller is further configured to determine the suction position of the dripping device according to the target medicine, and determine the dripping position of the dripping device in combination with the target slide heating zone; and generate a driving route of the dripping device according to the suction position and the dripping position.

4. The system according to claim 1, wherein: Partitions are provided on both sides of the slide heating zone for stabilizing the slide clamping piece, and the height of the partitions is greater than the thickness of the slide clamping piece.

5. The system according to claim 1, wherein: A limit baffle is provided on the side of the reaction tray, and a frame baffle aligned with the limit baffle is provided on the inner side of the shell of the tissue sample incubation area for limiting the extended length of the reaction tray.

6. The system according to claim 1, wherein: A drainage hole is provided on the bottom surface of the reaction tray, and a drip groove is slidably connected to the bottom surface at a position corresponding to the drainage hole. The drip groove has a notch, and a drainage pipe mouth is provided at the drainage hole. The drainage pipe mouth extends toward the drip groove and is provided at the far end of the notch.

7. A reaction method for immunohistochemical staining according to any one of claims 1 to 6, characterized in that: include: When a target slide holder is loaded with a plurality of tissue slides, a target slide identifier of the target slide holder is obtained by a controller, and a target slide heating zone in a tissue sample incubation zone is determined based on the target slide identifier, wherein the tissue sample incubation zone includes a multi-layer reaction tray, the reaction tray includes a plurality of slide heating zones, each of the slide heating zones is provided with a heater aligned with a slide slot on the slide holder, and the reaction tray enters and exits the tissue sample incubation zone via a conveying device provided on both sides of the tissue sample incubation zone; The controller drives the robotic arm according to the target slide identifier to place the slide holder carrying the target tissue slide in the target slide heating zone, and controls the target heater on the target slide heating zone to heat according to a preset heating threshold.

8. The method according to claim 7, characterized in that The tissue sample incubation area further includes a motor electrically connected to the conveying device, and configured to drive the conveying device according to an extension instruction or an entry instruction of the controller; querying, by the controller, a slide heating zone in an idle state in the reaction tray, and determining a target slide heating zone according to the idle state; The controller determines the conveying path of the robot arm to clamp the target slide clamp, and after driving the motor to drive the conveying device to extend the reaction tray corresponding to the target slide heating zone, the robot arm is driven according to the conveying path to clamp the slide clamp and place it in the target slide heating zone. The conveying path is a route determined based on the placement position of the target slide heating zone and the clamping position of the target slide clamp.

9. The method according to claim 7, characterized in that The tissue sample incubation area further includes a drug refrigeration area and a drug storage area disposed on the top layer of the reaction tray; the drug refrigeration area is used to provide cold air to the reaction tray; the drug storage area includes a plurality of drug storage slots for storing drug bottles; the drug storage slots include a first drug storage slot and a second drug storage slot, the first drug storage slots being arranged in a honeycomb pattern, and the second drug storage slots being arranged in parallel according to preset rows and preset columns; The controller determines a target dose and a target dripping amount according to the target slide identifier, determines a suction position of a dripping device for sucking and dripping the dose according to the target dose, and determines a dripping position of the dripping device in combination with the target slide heating zone; After the controller generates a driving route for the dripping device according to the suction position and the dripping position, the dripping device is driven to absorb medicine from the target medicine bottle and then drip the medicine onto the target tissue slide on the target slide heating area.

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