Mangrove forest ecological diversity assessment method and mangrove forest ecological diversity assessment system based on environmental DNA technology

Abstract

The invention relates to the technical field of environmental DNA, in particular to a mangrove forest ecological diversity assessment method and system based on an environmental DNA technology. Comprising the steps that a preset number of unit areas with the lowest ecological diversity serve as object areas; the geometric midpoints of the object areas are sequentially connected, so that the total length of connecting lines is shortest; constructing an evolution line and a reference line; taking a region defined by the reference line and the evolution line as a verification region; taking the area of the verification region as an evaluation value, presetting a critical threshold value, and if the evaluation value is smaller than or equal to the critical threshold value, determining the corresponding evolution line as a reference line; respectively extending two ends of the datum line to geometric midpoints of the two corresponding object areas to obtain an extended datum line; and taking a line formed by all the expanded datum lines as a monitoring datum line. According to the mangrove forest ecological subsystem, the ecological diversity is monitored, and meanwhile continuous tracking and positioning of the area with the low ecological diversity in the mangrove forest ecological subsystem are achieved.

Description

Technical Field

[0001] The present invention relates to the field of environmental DNA technology, and in particular to a mangrove ecological diversity assessment method and system based on environmental DNA technology. Background Art

[0002] Environmental DNA technology can be used to analyze ecological diversity, but it is still subject to many limitations in actual application, especially the lack of adaptability optimization for different ecological subsystems. For situations with different analysis needs, its universality is limited and its application flexibility cannot be expanded.

[0003] In view of this, this application is hereby filed. Summary of the invention

[0004] The first purpose of the present invention is to provide a mangrove ecological diversity assessment method based on environmental DNA technology, which can fully monitor the ecological diversity of mangrove ecological subsystems while realizing continuous tracking and positioning of areas with low ecological diversity in mangrove ecological subsystems, and can provide important reference data for improving the ecological diversity of mangrove ecological subsystems.

[0005] The second purpose of the present invention is to provide a mangrove ecological diversity assessment system based on environmental DNA technology, which can fully monitor the ecological diversity of mangrove ecological subsystems while realizing continuous tracking and positioning of areas with low ecological diversity in mangrove ecological subsystems, and can provide important reference data for improving the ecological diversity of mangrove ecological subsystems.

[0006] The embodiment of the present invention is achieved as follows:

[0007] A method for assessing mangrove ecological diversity based on environmental DNA technology comprises the following steps:

[0008] S1. Determine the area to be evaluated and divide it into several unit areas.

[0009] S2. Set up sampling points for each unit area and collect environmental samples at the sampling points.

[0010] S3. Use environmental DNA technology to analyze environmental samples and obtain ecological diversity data for each unit area.

[0011] S4. Determine a preset number of unit areas with the lowest ecological diversity according to the ecological diversity data as the target areas.

[0012] S5. Connect the geometric midpoints of the object area in sequence so that the total length of the connection is the shortest.

[0013] S6. In terms of the connection sequence, for two adjacent object areas, the intersection of the line segment between the two and the boundary between the two is used as a reference point, and the line between the two object areas is constructed along the boundary of the unit area as an evolution line. When constructing the evolution line, the length of the evolution line is made the shortest. A line is connected between the reference points of the two to obtain a reference line.

[0014] S7. Determine the area enclosed by the reference line and the evolution line as the verification area. If the reference line and the evolution line cannot close the verification area, the verification area is closed using the boundary of the unit area. When the verification area is closed using the boundary of the unit area, the area of ​​the verification area is minimized.

[0015] S8. The area of ​​the verification region is used as an evaluation value, and a critical threshold is preset. If the evaluation value is less than or equal to the critical threshold, the corresponding evolution line is identified as a baseline.

[0016] S9, respectively extending the two ends of the baseline to the geometric midpoints of the corresponding two object areas to obtain an extended baseline. The line formed by all the extended baselines is used as the monitoring baseline.

[0017] S10. Re-dividing the unit area in the area to be evaluated so that there is no part of the monitoring baseline that overlaps with the boundary of the unit area.

[0018] Furthermore, in S6, when constructing the evolution line, the boundary of the unit area with the lowest surrounding ecological diversity is preferentially selected for construction, and at the same time, it is ensured that the evaluation value is less than or equal to the critical threshold.

[0019] Furthermore, in S10, after the unit area is re-divided, S2-S10 are re-executed.

[0020] A mangrove ecological diversity assessment system based on environmental DNA technology includes: a memory and a processor.

[0021] The memory stores a computer program, and the computer program is configured to execute the above-mentioned mangrove ecological diversity assessment method based on environmental DNA technology when running;

[0022] The processor is configured to execute the above-mentioned mangrove ecological diversity assessment method based on environmental DNA technology through a computer program.

[0023] The beneficial effects of the technical solution of the embodiment of the present invention include:

[0024] The mangrove ecological diversity assessment method based on environmental DNA technology provided in an embodiment of the present invention can strengthen the key monitoring of the monitoring baseline by redividing the unit area, and can also synchronously calibrate and update the monitoring baseline, so as to facilitate long-term and continuous tracking of locations with low ecological diversity in the area to be assessed, thereby providing a reference basis for optimizing the ecological diversity in the area to be assessed, which is of positive significance for continuously optimizing the ecological diversity management work in the area to be assessed.

[0025] In general, the mangrove ecological diversity assessment method based on environmental DNA technology provided by the embodiment of the present invention fully monitors the ecological diversity of the mangrove ecological subsystem, while achieving continuous tracking and positioning of areas with low ecological diversity in the mangrove ecological subsystem, and can provide important reference data for improving the ecological diversity of the mangrove ecological subsystem. The mangrove ecological diversity assessment system based on environmental DNA technology provided by the embodiment of the present invention fully monitors the ecological diversity of the mangrove ecological subsystem, while achieving continuous tracking and positioning of areas with low ecological diversity in the mangrove ecological subsystem, and can provide important reference data for improving the ecological diversity of the mangrove ecological subsystem. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0027] Figure 1 A schematic diagram of constructing a unit area in the area to be evaluated;

[0028] Figure 2 A schematic diagram of a line segment between two adjacent object regions in the line sequence;

[0029] Figure 3 This is a schematic diagram for constructing the evolution line;

[0030] Figure 4 This is a schematic diagram for constructing the evolution line;

[0031] Figure 5 Schematic diagram of the extended baseline. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0033] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] It should be understood that the "system", "device", "unit" and / or "module" used in the present invention are a method for distinguishing different components, elements, parts, portions or assemblies at different levels. However, if other words can achieve the same purpose, the words can be replaced by other expressions.

[0035] As shown in this specification and claims, unless the context clearly provides an example, the words "a", "an", "the", etc. do not refer to the singular, but also include the plural. Generally speaking, the terms "include" and "comprise" only indicate the inclusion of the steps and elements that have been clearly identified, and these steps and elements do not constitute an exclusive list. The method or device may also include other steps or elements.

[0036] It is understood that the operations of each step are not necessarily performed precisely in order. Instead, the steps may be processed in reverse order or simultaneously. At the same time, other operations may be added to these processes, or one or more operations may be removed from these processes.

[0037] In order to overcome the shortcomings of the prior art, this embodiment provides a mangrove ecological diversity assessment method based on environmental DNA technology, which includes the following steps:

[0038] S1. Determine the area to be evaluated and divide it into several unit areas. Figure 1 As shown, the area to be evaluated is divided into 12 unit areas, namely unit area C1, unit area C2, unit area C3, unit area C4, unit area C5, unit area C6, unit area C7, unit area C8, unit area C9 and unit area C10. It should be noted that the specific division method of the unit area (area, number, shape, etc.) can be flexibly adjusted according to actual needs, and this application does not make specific restrictions. Figure 1 This is for illustrative purposes only and is not intended to be limiting.

[0039] S2. Set sampling points for each unit area and collect environmental samples at the sampling points. That is, set sampling points for collecting environmental samples in each unit area. The specific location and number of sampling points can be set according to specific circumstances, as long as they can reflect the ecological diversity of the unit area. This application does not make specific restrictions.

[0040] S3. Use environmental DNA technology to analyze environmental samples and obtain ecological diversity data for each unit area.

[0041] S4. Determine a preset number (the specific value can be flexibly set according to specific needs) of unit areas with the lowest ecological diversity based on the ecological diversity data as the target area.

[0042] S5. Connect the geometric midpoints of the object area in sequence so that the total length of the connection is the shortest.

[0043] S6. In terms of the connection order, for two adjacent object regions, such as Figure 2 As shown in FIG. 1 , unit area C3 and unit area C10 are two adjacent object areas in the order of connection. The intersection of the line segment between the two (i.e., line segment d1-d4) and the boundary between the two is taken as a reference point (point d2 and point d3, respectively), and the line between the two object areas (i.e., unit area C3 and unit area C10) is constructed along the boundary of the unit area as an evolution line. When constructing the evolution line, the length of the evolution line is minimized. The specifically constructed evolution line is as follows: Figure 3 As shown by the line segment d5-d6 in the figure. A line is drawn between the reference points (point d2 and point d3) of the two to obtain a reference line (as shown in FIG. Figure 3 (shown by the midline segment d2-d3).

[0044] S7. Determine the area enclosed by the reference line (line segment d2-d3) and the evolution line (line segment d5-d6) as the verification area. If the reference line and the evolution line cannot close the verification area, the verification area is closed using the boundary of the unit area. When the verification area is closed using the boundary of the unit area, the area of ​​the verification area is minimized. For line segments d2-d3 and line segments d5-d6, the area enclosed by them is not closed. Therefore, the corresponding verification area is closed with the help of line segments d2-d5 and line segments d3-d6, and the requirement of minimizing the area of ​​the verification area is met.

[0045] S8. The area of ​​the verification region is used as the evaluation value, such as Figure 3As shown, the area of ​​the verification area = the area of ​​△d2-d5-x + the area of ​​△d3-d6-x. A critical threshold is preset (the specific value can be flexibly set according to the actual situation). If the evaluation value is less than or equal to the critical threshold, the corresponding evolution line is identified as the baseline. This can avoid the physical distance between the evolution line and the baseline being too large. In particular, in S6, when constructing the evolution line, if there are multiple ways to construct the evolution line that meet the requirement of "the evaluation value is less than or equal to the critical threshold", the boundary of the unit area with the lowest surrounding ecological diversity is preferentially selected to construct the evolution line. As shown in Figure 4 As shown, if unit area C5 has the lowest ecological diversity in the surrounding area, then the broken line segment d5-d7-d8 can also be used as the evolution line between unit area C3 and unit area C10. At this time, the area enclosed by the evolution line (broken line segment d5-d7-d8) and the reference line (line segment d2-d3) needs to be closed with the help of line segments d8-d3 and d2-d5, and the area of ​​the corresponding verification area is equal to the area of ​​the polygon "d2-d7-d8-d3". If the area of ​​the polygon "d2-d7-d8-d3" is less than or equal to the critical threshold, the broken line segment d5-d7-d8 is used as the evolution line.

[0046] S9, extending the two ends of the reference line (taking the broken line segment d5-d7-d8 as an example) to the geometric midpoints of the two corresponding object areas to obtain an extended reference line. Figure 5 As shown, after extending the two ends of the broken line segment d5-d7-d8 respectively, the broken line segment d1-d5-d7-d8-d4 is obtained, which is the corresponding extended baseline. The endpoints of all extended baselines are at the geometric center of the object area, and adjacent extended baselines are connected to each other. The line formed by all extended baselines is used as the monitoring baseline. The monitoring baseline reflects the position line with lower ecological diversity in the entire area to be evaluated, that is, the position points on the monitoring baseline are at a lower level of ecological diversity in the area to be evaluated. The monitoring baseline can be used as a position indicator line for subsequent key monitoring.

[0047] S10, re-dividing the unit area in the area to be evaluated so that there is no part overlapping with the boundary of the unit area in the monitoring baseline. After the unit area is re-divided, S2-S10 can be repeated. Through this design, the key monitoring of the monitoring baseline (the position with lower ecological diversity) can be strengthened by re-dividing the unit area, and the monitoring baseline can also be calibrated and updated synchronously at the same time, so as to track the position with lower ecological diversity in the area to be evaluated continuously for a long time, thereby providing a reference basis for optimizing the ecological diversity in the area to be evaluated, which is of positive significance for continuously optimizing the ecological diversity management work in the area to be evaluated.

[0048] This embodiment also provides a mangrove ecological diversity assessment system based on environmental DNA technology, which includes: a memory and a processor.

[0049] The memory stores a computer program, and the computer program is configured to execute the above-mentioned mangrove ecological diversity assessment method based on environmental DNA technology when running;

[0050] The processor is configured to execute the above-mentioned mangrove ecological diversity assessment method based on environmental DNA technology through a computer program.

[0051] In summary, the mangrove ecological diversity assessment method based on environmental DNA technology provided by the embodiment of the present invention fully monitors the ecological diversity of the mangrove ecological subsystem, while achieving continuous tracking and positioning of areas with low ecological diversity in the mangrove ecological subsystem, and can provide important reference data for improving the ecological diversity of the mangrove ecological subsystem. The mangrove ecological diversity assessment system based on environmental DNA technology provided by the embodiment of the present invention fully monitors the ecological diversity of the mangrove ecological subsystem, while achieving continuous tracking and positioning of areas with low ecological diversity in the mangrove ecological subsystem, and can provide important reference data for improving the ecological diversity of the mangrove ecological subsystem.

[0052] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for assessing mangrove ecological diversity based on environmental DNA technology, characterized in that: The steps include: S1. Determine the area to be evaluated and divide the area to be evaluated into several unit areas; S2. Setting a sampling point for each unit area and collecting environmental samples at the sampling point; S3, analyzing the environmental samples using environmental DNA technology to obtain ecological diversity data of each unit area; S4, determining a preset number of unit areas with the lowest ecological diversity according to the ecological diversity data as target areas; S5, connecting the geometric midpoints of the object area in sequence so that the total length of the connection is the shortest; S6. In terms of the connection sequence, for two adjacent object areas, the intersection of the line segment between the two and the boundary between the two is used as a reference point, and a line between the two object areas is constructed along the boundary of the unit area as an evolution line; wherein, when constructing the evolution line, the length of the evolution line is made the shortest; and a line is connected between the reference points of the two to obtain a reference line; S7, determining an area enclosed by the reference line and the evolution line as a verification area; if the reference line and the evolution line cannot close the verification area, closing the verification area using the boundary of the unit area; wherein when closing the verification area using the boundary of the unit area, the area of ​​the verification area is minimized; S8, taking the area of ​​the verification region as an evaluation value, presetting a critical threshold, and if the evaluation value is less than or equal to the critical threshold, identifying the corresponding evolution line as a baseline; S9, respectively extending the two ends of the baseline to the geometric midpoints of the corresponding two object areas to obtain an extended baseline; and using the line formed by all the extended baselines as a monitoring baseline; S10: Re-divide the unit area in the area to be evaluated so that there is no part of the monitoring baseline that overlaps with the boundary of the unit area.

2. The method for assessing mangrove ecological diversity based on environmental DNA technology according to claim 1, characterized in that: In S6, when constructing the evolution line, the boundary of the unit area with the lowest surrounding ecological diversity is preferentially selected for construction, and at the same time, it is ensured that the evaluation value is less than or equal to the critical threshold.

3. The method for assessing mangrove ecological diversity based on environmental DNA technology according to claim 1, characterized in that: In S10, after the unit area is re-divided, S2-S10 are re-executed.

4. A mangrove ecological diversity assessment system based on environmental DNA technology, characterized in that: include: Memory and processor; The memory stores a computer program, and the computer program is configured to execute the mangrove ecological diversity assessment method based on environmental DNA technology according to any one of claims 1 to 3 when running; The processor is configured to execute the mangrove ecological diversity assessment method based on environmental DNA technology as described in any one of claims 1 to 3 through the computer program.

Images