High-altitude layering propagation method for dalbergia odorifera and application of high-altitude layering propagation method

Abstract

The invention provides a dalbergia odorifera high-altitude layering propagation method and application thereof, and belongs to the technical field of plant cultivation. The method comprises the following steps: selecting a healthy dalbergia odorifera seed tree which is more than 3 years old, girdling branches which are more than or equal to 1 year old until xylem is reached, and then scraping off cambium; a rooting agent A is coated at the cut, a substrate containing a rooting agent B and a bactericide is fixed through a high-pressure box, and bacterial manure is sprayed at the rooting position after 8-10 days. The rooting agent A is a naphthylacetic acid solution and an indoleacetic acid solution, the rooting agent B is indolebutyric acid, and the bacterial fertilizer comprises trichoderma asperellum and oxaloacetic acid decarboxylase. According to the method, rapid rooting of the dalbergia odorifera can be promoted, the mean value of the rooting rate is larger than 98%, vegetative propagation of the dalbergia odorifera can be achieved, and rapid propagation of good germplasm is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cultivation and planting, and particularly relates to an air layering propagation method for Dalbergia odorifera and its application. Background Art

[0002] Dalbergia odorifera T.Chen is a plant of the genus Dalbergia in the legume family, also known as Huanghuali, Huali mother, etc. Its heartwood has a dense structure, beautiful texture and a special fragrant smell, and is an excellent raw material for furniture and handicrafts. The heartwood of Dalbergia odorifera can extract various secondary metabolites such as flavonoids and nerolidol, which have antibacterial, anti-inflammatory, analgesic and other effects and are widely used in the medical and pharmaceutical industries. Due to the extremely high value of Dalbergia odorifera and the scarcity of wild resources, it has been listed as a national second-class protected wild plant. At present, Dalbergia odorifera is mainly planted artificially. Since Dalbergia odorifera has always been propagated by seeds, seed propagation has the following disadvantages: flowering and fruiting are relatively late, resulting in a longer cultivation cycle. The new individuals propagated by seeds have the traits of both parents, which may lead to complex offspring traits and is not conducive to meeting certain specific requirements, resulting in inconsistent and unstable heartwood formation. Air layering is an important way for variety improvement. Through the air layering of excellent varieties, not only the high-quality germplasm resources of Dalbergia odorifera are protected, but also the quality of the heartwood is improved. Air layering has the advantages of maintaining the excellent traits of the mother plant, simple operation, economic feasibility, etc., and is an important means for the propagation of endangered tree species and excellent germplasm. Summary of the Invention

[0003] Based on the existing technology, the technical problem to be solved by the present invention is to provide an air layering propagation method for Dalbergia odorifera to solve the above problems.

[0004] The technical solution of the present invention: An air layering propagation method for Dalbergia odorifera, comprising the following steps:

[0005] S1. Select Dalbergia odorifera as the mother tree, select branches ≥ 1 year old on healthy mother trees over 3 years old for circumcision until reaching the xylem, and scrape off the cambium;

[0006] S2. After scraping, apply rooting agent A to the cut, and fix the substrate containing rooting agent B and fungicide through a high-pressure box. Spray the microbial fertilizer at the rooting site 8 - 10 days later.

[0007] Further, in step S1, the circumcision diameter is 1.4 - 1.6 times the diameter of the branch.

[0008] Further, in step S2, the rooting agent A is a naphthaleneacetic acid solution and an indoleacetic acid solution with a mass ratio of 1 - 1.5:1 - 2, the volume concentration of the naphthaleneacetic acid solution is 100 - 300 mg / L, and the volume concentration of the indoleacetic acid solution is 100 - 300 mg / L.

[0009] Further, in step S2, the rooting agent B is indolebutyric acid, and the volume concentration of the indolebutyric acid is 100 mg / L.

[0010] Further, in step S2, the bacterial fertilizer includes Trichoderma asperellum and oxaloacetate decarboxylase with a mass ratio of 1-2:1-3; the spraying method is to dissolve the bacterial fertilizer in water according to a mass ratio of 1-1.5:200-300, take the supernatant, and spray it on the rooting part and the substrate after filtration.

[0011] Further, in step S2, the fungicide is one or two of mancozeb, chlorothalonil, carbendazim, and jinggangmycin.

[0012] Further, in step S2, the substrate is perlite, vermiculite, peat soil, and rice flour with a mass ratio of 3-5:2-2.5:6-8:1-2.

[0013] Further, the rice flour is ground from one or two of rice, glutinous rice, japonica rice, and indica rice, and the grinding particle size is 0.4-0.6 mm.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0015] (1) The Dalbergia odorifera propagated by the method of the present invention can promote the rapid rooting of Dalbergia odorifera, significantly increase the number of roots per plant, and realize the rapid propagation of excellent germplasm.

[0016] (2) The Dalbergia odorifera propagated by the method of the present invention reduces the risk of root rot, increases the rooting rate of Dalbergia odorifera, and increases the asexual propagation efficiency of Dalbergia odorifera. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 : Rooting of aerial layering propagation of Dalbergia odorifera in Example 1 for 30 days.

[0018] Figure 2 : Rooting of aerial layering propagation of Dalbergia odorifera in Example 2 for 30 days.

[0019] Figure 3 : Rooting of aerial layering propagation of Dalbergia odorifera in Example 3 for 30 days.

[0020] Figure 4 : Rooting of aerial layering propagation of Dalbergia odorifera in Comparative Example 1 for 30 days. DETAILED DESCRIPTION OF THE INVENTION

[0021] In order to better understand the technical content of the present invention and describe the technical solution clearly and completely, specific embodiments are provided below to further illustrate the present invention. The following are only some embodiments of the present invention.

[0022] The materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial channels without special instructions.

[0023] In this embodiment, the raw material components are as follows: Trichoderma asperellum was purchased from Shandong Hezhong Kangyuan Biotechnology Co., Ltd., and the effective viable count is greater than 100 billion CFU / g; oxaloacetate decarboxylase was purchased from Shanghai Yuanye Biotechnology Co., Ltd., with the CAS number 9024-98-0, and the viable count is greater than 12 billion CFU / g.

[0024] Rooting rate % = total number of rooted plants / total number of plants cultured for rooting × 100%

[0025] Example 1

[0026] A method for aerial layering propagation of Dalbergia odorifera T. Chen includes the following steps:

[0027] Step (1): Select Dalbergia odorifera T. Chen as the mother tree. On the mother tree, select a healthy Dalbergia odorifera T. Chen with a tree age of 10 years. Perform girdling on the 1-year-old branches. The girdling diameter is 1.5 times the diameter of the branches. Girdle until reaching the xylem and scrape off the cambium.

[0028] Step (2): After scraping, apply rooting agent A to the cut. Rooting agent A is a naphthaleneacetic acid solution and an indoleacetic acid solution with a mass ratio of 1:1. Among them, the volume concentrations of the naphthaleneacetic acid solution and the indoleacetic acid solution are both 100 mg / L. And fix the substrate containing rooting agent B indolebutyric acid (volume concentration of 100 mg / L) and fungicides (chlorothalonil and carbendazim) through a high-pressure box. The substrate is perlite, vermiculite, peat soil and rice with a mass ratio of 3:2:6:1. Among them, the grinding particle size of the rice is 0.4 mm. After 8 - 10 days of operation, spray microbial fertilizer at the rooting site. The microbial fertilizer is composed of Trichoderma asperellum and oxaloacetate decarboxylase with a mass ratio of 1:1.5. The specific spraying method is to dissolve the microbial fertilizer in water according to a mass ratio of 1:200, filter the supernatant after dissolution, and spray the filtrate at the rooting site and into the substrate.

[0029] Example 2

[0030] A method for aerial layering propagation of Dalbergia odorifera T. Chen includes the following steps:

[0031] Step (1): Select Dalbergia odorifera T. Chen as the mother tree. On the mother tree, select a healthy Dalbergia odorifera T. Chen with a tree age of 10 years. Perform girdling on the 1-year-old branches. The girdling diameter is 1.5 times the diameter of the branches. Girdle until reaching the xylem and scrape off the cambium;

[0032] Step (2): After scraping, apply rooting agent A on the well-treated cut. Rooting agent A is composed of naphthalene acetic acid solution (volume concentration: 200 mg / L) and indoleacetic acid solution (volume concentration: 200 mg / L) with a mass ratio of 1:1. Fix the substrate containing rooting agent B and fungicide through a high-pressure box. Among them, rooting agent B is indolebutyric acid (volume concentration: 100 mg / L), and the fungicide is chlorothalonil. The substrate is perlite, vermiculite, peat soil, and japonica rice with a mass ratio of 5:2.5:8:2, and the grinding particle size of japonica rice is 0.6 mm. After 8 - 10 days of operation, spray the microbial fertilizer at the rooting part. The microbial fertilizer is composed of Trichoderma asperellum and oxaloacetate decarboxylase with a mass ratio of 2:3. The specific spraying method is to dissolve the microbial fertilizer in water according to a mass ratio of 1.5:300, take the supernatant after dissolution and filter it, and spray the filtrate at the rooting part and into the substrate.

[0033] Example 3

[0034] A method for aerial layering propagation of Dalbergia odorifera T. Chen includes the following steps:

[0035] Step (1): Select Dalbergia odorifera T. Chen as the mother tree, choose healthy Dalbergia odorifera T. Chen branches with a tree age of 10 years on the mother tree, perform circumcision on the one-year-old branches, the circumcision diameter is 1.5 times the diameter of the branches, the circumcision reaches the xylem, and scrape off the cambium;

[0036] Step (2): After scraping, apply rooting agent A on the well-treated cut. Rooting agent A is composed of naphthalene acetic acid solution (volume concentration: 300 mg / L) and indoleacetic acid solution (volume concentration: 300 mg / L) with a mass ratio of 1:1. Fix the substrate containing rooting agent B and fungicide through a high-pressure box. Among them, rooting agent B is indolebutyric acid (volume concentration: 100 mg / L). The fungicide is jinggangmycin. The substrate is perlite, vermiculite, peat soil, and indica rice with a mass ratio of 4:2:7:1, and the grinding particle size of indica rice is 0.5 mm. After 8 - 10 days of operation, spray the microbial fertilizer at the rooting part. The microbial fertilizer is composed of Trichoderma asperellum and oxaloacetate decarboxylase with a mass ratio of 1.5:2. The specific spraying method is to dissolve the microbial fertilizer in water according to a mass ratio of 1:250, take the supernatant after dissolution and filter it, and spray the filtrate at the rooting part and into the substrate.

[0037] Comparative Example 1

[0038] The difference between Comparative Example 1 and Example 3 is that in Step (2), the volume concentrations of naphthalene acetic acid solution and indoleacetic acid solution are reduced to 50 mg / L, and the volume concentration of indolebutyric acid is 50 mg / L. Other methods and steps are the same as those in Example 3.

[0039] Comparative Example 2

[0040] The difference between Comparative Example 2 and Example 3 is that in step (ii), rooting agent B indolebutyric acid is removed, and other methods and steps are the same as those in Example 3.

[0041] Comparative Example 3

[0042] The difference between Comparative Example 3 and Example 3 is that in step (ii), sterilized fertilizer is removed from the substrate raw materials, and the fungicide is removed, and other methods and steps are the same as those in Example 3.

[0043] Experiment:

[0044] Randomly select 120 healthy Dalbergia odorifera T. Chen branches. Each group of the examples and comparative examples has 20 tests, and they are propagated according to the different air layering propagation methods of the examples and comparative examples. Count the roots of the air layering of Dalbergia odorifera T. Chen propagated by the methods of Examples 1-3 and Comparative Examples 1-3. On the 30th day, all the roots are cleaned, and the number of roots on the 30th day is counted. See Table 1.

[0045] The results show that the number of roots of Dalbergia odorifera T. Chen on the 30th day propagated by the propagation methods of Examples 1-3 is significantly higher than that of Comparative Examples 1-3. After the volume concentrations of naphthaleneacetic acid solution and indoleacetic acid solution in Comparative Example 1 were reduced to 50 mg / L and rooting agent B indolebutyric acid was removed in Comparative Example 2, the number of roots on the 30th day decreased significantly compared with Example 3; after the applied fertilizer, polished rice, and fungicide were removed in Comparative Example 3, the number of roots decreased significantly. Therefore, the air layering propagation method of Dalbergia odorifera T. Chen of the present invention can significantly increase the number of roots of the branches, and there is no phenomenon of rotten roots.

[0046] The calculation results of the rooting rate show that the number of roots per plant in Examples 1-3 is 140-160, and the average rooting rate is 85%-95%. That is, the number of roots and the rooting rate of Dalbergia odorifera T. Chen propagated by the propagation methods of Examples 1-3 are higher than those of Comparative Examples 1-3. That is, the air layering propagation method of Dalbergia odorifera T. Chen of the present invention improves the number of roots and the rooting rate of Dalbergia odorifera T. Chen.

[0047] Table 1 Statistical table of rooting rate

[0048]

[0049] The test results show that the number of roots of Dalbergia odorifera propagated by the propagation methods of Examples 1-3 on the 30th day is significantly higher than that of Comparative Examples 1-3. In Comparative Example 1, when the volume concentrations of the naphthalene acetic acid solution and the indole acetic acid solution were reduced, callus could be formed on the branches of Dalbergia odorifera, but it was not easy to take root, and the number of roots decreased significantly. In Comparative Example 2, after removing rooting agent B indolebutyric acid, callus could be formed on the branches of Dalbergia odorifera, but it was not easy to take root, and the number of roots decreased significantly. In Comparative Example 3, after removing the applied bacterial fertilizer, removing the indica rice, and removing the fungicide, the wound of the branches of Dalbergia odorifera was prone to mildew and not easy to take root, and there was a phenomenon of rotten roots in individual roots. The rooting rate of Dalbergia odorifera propagated by the propagation methods of Examples 1-3 is higher than that of Comparative Examples 1-3. That is, the aerial layering propagation method of Dalbergia odorifera of the present invention improves the number of roots and the rooting rate of Dalbergia odorifera. Therefore, the present invention can promote the rapid rooting of Dalbergia odorifera, can realize the asexual reproduction of Dalbergia odorifera, and can realize the rapid reproduction of excellent germplasm.

[0050] The foregoing are only the exemplary embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for propagating Dalbergia odorifera through high-altitude layering, characterized in that: The steps include: S1. Select Dalbergia odorifera as the mother tree, and select branches ≥1 year old from healthy mother trees over 3 years old for girdling, reaching the xylem, and scraping off the cambium; S2. Apply rooting agent A to the cut after scraping, and fix the matrix containing rooting agent B and fungicide through a high-pressure box. After 8-10 days, spray bacterial fertilizer on the rooting area.

2. The method for propagating Dalbergia odorifera through high-altitude layering as claimed in claim 1, characterized in that: Step S1, the ring cutting diameter is 1.4-1.6 times the branch diameter.

3. The method for high-altitude layering propagation of Dalbergia odorifera according to claim 1, characterized in that: Step S2, the rooting agent A is a naphthaleneacetic acid solution and an indoleacetic acid solution in a mass ratio of 1-1.5:1-2, the volume concentration of the naphthaleneacetic acid solution is 100-300 mg / L, and the volume concentration of the indoleacetic acid solution is 100-300 mg / L.

4. The method for high-altitude layering propagation of Dalbergia odorifera according to claim 1, characterized in that: Step S2, the rooting agent B is indolebutyric acid, and the volume concentration of the indolebutyric acid is 100 mg / L.

5. The method for propagating Dalbergia odorifera through high-altitude layering as claimed in claim 1, characterized in that: Step S2, the bacterial fertilizer includes Trichoderma spinulosa and oxaloacetate decarboxylase in a mass ratio of 1-2:1-3; the spraying method is to dissolve the bacterial fertilizer in water in a mass ratio of 1-1.5:200-300, take the supernatant, filter it, and then spray it on the rooting area and the matrix.

6. The method for propagating Dalbergia odorifera through high-altitude layering as claimed in claim 1, characterized in that: Step S2, the fungicide is one or two of mancozeb, thiophanate-methyl, carbendazim and jinggangmycin.

7. The method for propagating Dalbergia odorifera through high-altitude layering as claimed in claim 1, characterized in that: Step S2, the matrix is ​​perlite, vermiculite, peat soil and rice flour in a mass ratio of 3-5:2-2.5:6-8:1-2.

8. The method for high-altitude layering propagation of Dalbergia odorifera according to claim 7, characterized in that: The rice flour is ground from one or two of rice, glutinous rice, polished round-grained rice and indica rice, and the grinding particle size is 0.4-0.6 mm.

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