Sterilization composition containing Xinjunan and JS399-19 and application thereof

Abstract

The invention discloses a bactericidal composition containing Xinjunan and JS399-19 and application of a preparation. The active components of the bactericidal composition are Xinjunan and JS399-19 in a mass ratio of 1: 9-9: 1. Compared with single use of the two components, the bactericidal composition disclosed by the invention has high bactericidal activity, delays the generation and development of drug resistance of pathogens, reduces the risk that pathogenic fungi generate resistance to a single bactericide, and has the characteristics of economy, high efficiency, safety and environmental protection.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural fungicides, in particular to a fungicide composition containing cinclostrobin and cyproconazole and application thereof. Background Art

[0002] So far, no rice variety that is completely immune to Bakanae disease has been found, and the number of rice varieties resistant to Bakanae disease screened from rice germplasm resources is not large. Therefore, rice Bakanae disease still relies on chemical control methods of treating seeds with fungicides in production. At present, the main agents for the prevention and control of rice Bakanae disease in production are benzimidazole fungicides represented by carbendazim and ergosterol biosynthesis inhibitors represented by prochloraz. However, rice Bakanae disease fungi have developed varying degrees of resistance to carbendazim and prochloraz, which greatly reduces the control effect.

[0003] Wheat fusarium head blight is a widely distributed disease that occurs in almost all wheat-producing areas around the world, and is particularly serious in mild and humid areas. Due to the lack of highly efficient disease-resistant varieties and effective prevention and control measures, the prevention and control of wheat fusarium head blight in production still mainly relies on chemical control. At present, the main agents for the prevention and control of wheat fusarium head blight in production are benzimidazole fungicides represented by carbendazim and sterol biosynthesis inhibitors represented by tebuconazole. However, due to the large-scale and long-term single use of the above agents, wheat fusarium head blight fungi have developed very serious resistance to some agents. How to continue to control rice seedling disease and wheat fusarium head blight, especially to control the epidemic hazards of drug-resistant strains, has become a major issue facing the majority of scientific and technological workers and growers.

[0004] Sinafenamide belongs to the alkyl polyamine fungicide class, which has both systemic and protective effects. It can be absorbed by plant tissues and conducted in the plant body, providing long-term disease protection. Compared with ordinary fungicides, it is more efficient in plant disease prevention and control, and can provide more lasting protection during the high incidence of diseases, slowing down the spread of diseases. Sinafenamide is mainly used to prevent and control a variety of diseases on crops such as rice, vegetables, and fruit trees, such as rice bacterial blight, tomato bacterial spot, and apple scab.

[0005] Cyanostrobin is a highly effective cyanoacrylate fungicide with a novel structure and unique mode of action. The fungicide has strong specificity and high fungicidal activity, and has good protective and therapeutic effects on plant diseases caused by pathogenic bacteria. It is widely used in global agricultural production to prevent and control fungal diseases of various crops, especially watermelon wilt caused by Fusarium.

[0006] Both cyproconazole and cyanothiocarb are used alone to inhibit fungi, but long-term use of the same agent for disease control alone will increase the risk of field resistance. The Fungicide Resistance Committee (FRAC) recommends that the use of agents with different mechanisms of action can avoid or delay the development of resistance. Therefore, combining the two into a fungicidal composition can not only effectively solve the practical production problems of rice seedling blight and wheat scab that are difficult to prevent and control, but also delay the development of resistance to them by pathogens. The inventor compounded cyproconazole with cyanothiocarb and found that it has excellent control effects on rice seedling blight and wheat scab within a suitable range, can significantly improve the prevention effect, reduce the number of medications and the amount of medication, slow down the development of pathogen resistance, and reduce the cost of prevention and control. Summary of the invention

[0007] In order to solve the problems existing in the prior art, the inventors compounded cyproconazole and cyanothiocarb in a certain mass ratio to produce a synergistic effect, which not only effectively reduced the amount of pesticides used, but also effectively reduced the cost of prevention and control, and at the same time greatly reduced the pesticide residues in agricultural products. Based on the above research, the applicants completed the present invention.

[0008] The present invention combines two types of fungicides with different action mechanisms and processes them into a preparation that is easy to use in production. Compared with the two components used separately, the fungicide composition has high biological activity, and the total dosage per unit area is reduced, which greatly reduces the cost of drug application. At the same time, it delays the generation and development of pathogen resistance to a greater extent, reduces the risk of pathogenic fungi developing resistance to a single fungicide, and prolongs the service life of each component in the fungicide composition.

[0009] To achieve the above object, the present invention provides the following technical solutions:

[0010] The first aspect of the present invention provides a fungicide composition, wherein the active ingredients are composed of sinotriptyline and cyproconazole; based on the above scheme, the mass ratio of sinotriptyline to cyproconazole is 1:9 to 9:1; based on the above scheme, the preferred mass ratio of sinotriptyline to cyproconazole is 1:1 to 9:1; more preferably 3:1.

[0011] The second aspect of the present invention provides a preparation of a fungicide composition containing cinnamyl and cyanoacrylate. In addition to the active ingredients, the fungicide composition contains auxiliary agents, and the mass percentage of the active ingredients in the fungicide composition is 20% to 30%.

[0012] The bactericidal composition containing cinnamyl and cyanoacrylate is characterized in that the dosage form of the bactericidal composition is emulsifiable concentrate and suspension.

[0013] The third aspect of the present invention provides an application of the preparation in preventing and controlling rice seedling blanching disease and wheat scab.

[0014] By adopting the above technical solution, the present invention has the following beneficial effects:

[0015] (1) The present invention has a higher control effect on rice seedling blight and wheat fusarium head blight, and the fungicide composition containing cinclostrobin and cyanocobalamin has a significant synergistic effect compared with a single agent; (2) It reduces the amount of medicine used, effectively reduces ecological damage and environmental pollution, and can improve crop yield and quality; (3) It effectively controls the resistance of pathogens such as Fusarium to fungicides such as carbendazim and prochloraz, and can be used in rotation with a variety of existing agents, delaying the development and spread of pathogen resistance to existing agents and single agents, and is of great significance for the comprehensive control of multiple diseases. DETAILED DESCRIPTION

[0016] The specific scheme and effects of the present invention are further disclosed below in conjunction with specific embodiments.

[0017] Example 1: Indoor bioassay of cyproconazole and cyproconazole against rice seedling pathogen

[0018] Test bacteria: Rice seedling pathogen (Fusarium fujikuroi) was provided by the Fungicide Biology Laboratory of Anhui Agricultural University.

[0019] Drug preparation: The test drug was dissolved in acetone to prepare a single-dose stock solution and mixed stock solutions of various proportions. Five mass concentration gradients were set for each single dose and mixed stock solution according to the equal ratio method.

[0020] Test method: Refer to the pesticide indoor bioassay test guidelines NY / T 1156.2-2006, use the plate method, cut the pure culture of rice seedling pathogen from the edge of the colony with a sterile puncher with a diameter of 5mm under sterile conditions, and inoculate the cake with an inoculator in the center of the drug-containing plate, with the mycelium facing down, and repeat 4 times for each treatment. Place it in a constant temperature incubator at 25±1℃ for cultivation. When the diameter of the blank control colony is about 5cm, measure the colony diameter with a digital vernier caliper. Measure the diameter of each colony vertically once using the cross method, and take the average value.

[0021] Data analysis: According to the survey results, the growth inhibition rate of each treatment was calculated according to the formula. The calculation formula is as follows:

[0022] Growth inhibition rate (%) = (control colony diameter - treated colony diameter) / (control colony diameter - bacterial cake diameter) × 100

[0023] Based on the logarithmic value of the concentration of the drug and the probability value of the corresponding bacterial growth inhibition rate, the DPS data processing system was used for regression analysis to calculate the EC of each drug. 50 value.

[0024] Based on the EC of two single agents 50The combination ratio of the two drugs is set according to the Wadley method, and the synergistic coefficient (SR) is calculated according to the Wadley method, and a comprehensive evaluation is made based on this. SR ≤ 0.5 indicates an antagonistic effect, 0.5 < SR < 1.5 indicates an additive effect, and SR ≥ 1.5 indicates a synergistic effect. The SR value calculation method is as follows:

[0025] EC 50 (theoretical value) = (a+b) / (a / EC 50A +b / EC 50B );

[0026] SR=EC 50 (Theoretical value) / EC 50 (actual value);

[0027] SR is the synergistic coefficient, a and b are the percentages of each component in the mixture.

[0028] Results and analysis:

[0029] The results of indoor bioassay (Table 1) showed that the EC values ​​of cyproconazole and cyproconazole against rice seedling pathogen were 50 The results show that the synergistic effect of the compound combination of sinotriptyline and cyanothiocarb was 10.5609 mg / L and 0.2185 mg / L respectively. When the mass ratio of sinotriptyline to cyanothiocarb was 9:1, 7:1, 5:1, 3:1, 1:1, 1:3, 1:5, 1:7 and 1:9, the synergistic coefficient (SR) was 1.6161, 1.8040, 1.7539, 2.8794, 1.5934, 1.3254, 0.9828, 1.2057 and 1.1623 respectively. This shows that the compound combination of sinotriptyline and cyanothiocarb with a mass ratio of 9:1 to 1:1 showed a synergistic effect on rice seedling pathogen, especially when the mass ratio of sinotriptyline to cyanothiocarb was 3:1, the synergistic coefficient was greater than 2.5, and the synergistic effect was most obvious.

[0030] Table 1 Indoor bioassay results of the combination of cinclostrobin and cyproconazole against rice seedling pathogen

[0031]

[0032] Example 2: Preparation Example

[0033] Example 1: Preparation of 30% cyproconazole-cyanoacrylate emulsifiable concentrate

[0034] Calculated by mass percentage, the distribution ratio of each group is: 15% of cinclostrobin, 15% of cyproconazole, 25% of cyclohexane (solvent), 4% of calcium dodecylbenzenesulfonate (emulsifier), 6% of castor oil polyethylene glycol condensate (emulsifier), 5% of glycerol (antifreeze agent), and xylene (filler) makes up the balance.

[0035] Preparation method: Prepare the materials according to the above ratio and add them into a mixing kettle through a conventional emulsifiable concentrate processing method, and stir evenly to prepare a 30% cypermethrin-butyl emulsifiable concentrate.

[0036] Example 2: Preparation of 20% cyproconazole-cyanoacrylate emulsifiable concentrate

[0037] Calculated by mass percentage, the distribution ratio of each group is: 15% of cinclostrobin, 5% of cyproconazole, 25% of cyclohexane (solvent), 4% of calcium dodecylbenzenesulfonate (emulsifier), 6% of castor oil polyethylene glycol condensate (emulsifier), 5% of propylene glycol (antifreeze agent), and xylene (filler) makes up the balance.

[0038] Preparation method: Prepare the materials according to the above ratio and add them into a mixing kettle through a conventional emulsifiable concentrate processing method, and stir evenly to prepare a 20% cypermethrin-butyl emulsifiable concentrate.

[0039] Example 3: Preparation of 24% cyproconazole-cyanoacrylate suspension

[0040] Calculated by mass percentage, the distribution ratio of each group is: 20% of cintamidine, 4% of cyproconazole, 3% of sodium alkylnaphthalene sulfonate (dispersant), 3% of fumed silica (thickener), 2% of butyl epoxy stearate (stabilizer), 1% of silicone (defoaming agent), 1% of propylene glycol (antifreeze agent), and water makes up the balance.

[0041] Preparation method: Prepare the materials according to the above ratio and use the conventional suspension preparation method to prepare 24% cyproconazole-cyanoacrylate suspension.

[0042] Example 4: Preparation of 24% cyproconazole-cyanoacrylate suspension

[0043] Calculated by mass percentage, the distribution ratio of each group is: 21% of cincomycin, 3% of cyproconazole, 3% of sodium alkylnaphthalene sulfonate (dispersant), 3% of fumed silica (thickener), 2% of butyl epoxy stearate (stabilizer), 1% of silicone (defoaming agent), 1% of propylene glycol (antifreeze agent), and water makes up the balance.

[0044] Preparation method: Prepare the materials according to the above ratio and use the conventional suspension preparation method to prepare 24% cyproconazole-cyanoacrylate suspension.

[0045] Example 5: Preparation of 30% cyproconazole-cyanoacrylate suspension

[0046] Calculated by mass percentage, the distribution ratio of each group is: 27% cintamidine, 3% cyproconazole, 3% sodium alkylnaphthalene sulfonate (dispersant), 3% fumed silica (thickener), 2% butyl epoxy stearate (stabilizer), 1% silicone (defoaming agent), 1% propylene glycol (antifreeze agent), and water makes up the balance.

[0047] Preparation method: Prepare the materials according to the above ratio and use the conventional suspension preparation method to prepare 30% cyproconazole-cyanoacrylate suspension.

[0048] Example 3: Field control effect

[0049] In order to further verify the practical application effect of the present invention, the fungicidal compositions containing cincomycin and cyproconazole in Preparation Examples 1-5 were respectively used to carry out field efficacy tests, with cincomycin and cyproconazole as control agents.

[0050] Field prevention effect use case 1:

[0051] Experimental treatment method: The field control test of rice seedling blight was conducted in the suburbs of Bengbu City, where rice seedling blight occurred severely in previous years. The test area was 0.9 hm 2 Each treatment was repeated three times. Before sowing rice, the seeds were soaked with the above-mentioned agent solution, and then moved to a constant temperature of 30°C for germination. After germination, the seeds were sown.

[0052] Efficacy investigation and calculation method: The investigation on rice seedling rot was carried out in accordance with the "Guidelines for Field Efficacy Tests of Pesticides (II): Fungicides for Control of Rice Seedling rot" (GB / T17980.104-2004). The investigation was carried out before heading, and five random sampling points were taken in each plot. Twenty clusters were investigated at each point. The total number of investigated plants and the number of diseased plants were recorded, and the control effect was calculated based on the diseased plant rate. Among them, the rice seedling rot-infected seedlings were taller and thinner than the healthy seedlings, with narrow and long leaves and leaf sheaths, and light yellow-green leaf color.

[0053] Diseased plant rate = (number of diseased plants / total number of plants) × 100%;

[0054] Control effect = (number of diseased plants in the control area - number of diseased plants in the treatment area) / number of diseased plants in the control area × 100%.

[0055] Results and Analysis:

[0056] The results (Table 2) show that the fungicidal composition containing cinclostrobin and cyanocobalamin exhibits excellent control effect on rice seedling bakkan. Compared with the control agents cinclostrobin (control effect: 42.13%) and cyanocobalamin (control effect: 60.33%), the control effects of Examples 1-5 are all higher than 80%. At the same dosage, the control effect of each treatment of the fungicidal composition is significantly higher than that of the control agent, showing a significant synergistic effect.

[0057] Table 2 Sinclozolin · Field efficacy of cyanobacterium-butyl fungicide combination for seed soaking against rice bakanae disease

[0058]

[0059] Field prevention effect use case 2:

[0060] Experimental treatment method: The field prevention test of wheat fusarium rust was carried out in the suburbs of Bengbu City. The pesticide was sprayed during the flowering period of wheat, and the spraying of clean water was used as a control. The pesticide was applied twice with an interval of 7 days, and each treatment was repeated three times.

[0061] Efficacy investigation and calculation method: After the occurrence of ergot disease stabilizes, investigate the incidence of the disease, use five-point diagonal sampling, and investigate 100 ears for each treatment.

[0062] The severity classification standards for diseased ears are:

[0063] Level 1 diseased spikelets account for less than 25% of the whole spike.

[0064] Level 2 diseased spikelets account for 25% to 50% of the entire spike.

[0065] Grade 3 diseased spikelets account for 50% to 75% of the entire spike.

[0066] Level 4 diseased spikelets account for more than 75% of the whole spike. The disease index and control effect calculation formula are:

[0067] Disease index = [Σ(number of diseased ears at each level × corresponding disease level value) / total number of ears surveyed × highest level representative value] × 100;

[0068] Control effect = [(control disease index - treatment disease index) / control disease index] × 100%.

[0069] Results and analysis:

[0070] The results (Table 3) show that the fungicidal composition containing cinclostrobin and cyanocobalamin exhibits excellent control effect on wheat fusarium head blight, and the control effect of Examples 1-5 is higher than 80%. At the same dosage, the control effect of each treatment of the fungicidal composition is higher than that of the control agent, showing a certain synergistic effect.

[0071] Table 3 Sinclozolin · Field efficacy of cyanobacterium-butyl fungicide combination in controlling wheat fusarium head blight

[0072]

[0073] The results of the indoor activity test and the field test above show that the fungicidal composition containing cinclostrobin and cyanobacterium methyl in the present invention shows good control effect on rice bakanae disease and wheat scab, and the obtained fungicidal composition or preparation shows obvious synergistic effect. The fungicidal composition obtained by compounding the present invention can delay the resistance of rice bakanae disease and wheat scab to a single agent, reduce the dosage of the drug, effectively reduce ecological damage and environmental pollution, and can improve crop yield and quality.

[0074] Finally, it should be noted that the above examples are only some specific embodiments of the present invention. Obviously, the present invention is not limited to the above embodiments, and there are many variations. All variations that can be directly derived or associated with the content disclosed by a person skilled in the art are considered to be within the scope of protection of the present invention.

Claims

1. A fungicidal composition containing cincomycin and cyproconazole, characterized in that: The active ingredients are composed of cincomycin and cyproconazole, and the mass ratio of cincomycin to cyproconazole is 1:1 to 9:

1.

2. The bactericidal composition according to claim 1, characterized in that: The mass ratio of cinclostrobin to cyproconazole in the bactericidal composition is 1:1, 3:1, 5:1, 7:1, and 9:

1.

3. The bactericidal composition according to claim 1, characterized in that: The mass ratio of cinclostrobin to cyanocbiconamide in the bactericidal composition is 3:

1.

4. The bactericidal composition according to claim 1, characterized in that: Also contains agricultural auxiliary ingredients.

5. The bactericidal composition according to claim 4, characterized in that: The dosage form of the bactericidal composition is emulsifiable concentrate, suspension, microemulsion, aqueous emulsion, water-dispersible granules and wettable powder.

6. Use of the fungicidal composition according to any one of claims 1 to 5 for preventing and controlling crop diseases.

7. The use according to claim 6, characterized in that The crop diseases are rice seedling blight and wheat scab.

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