Crop seed coating agent, method for promoting drought-resistant germination of crops and application of crop seed coating agent

Abstract

The invention belongs to the technical field of seed coating agents, and particularly relates to a crop seed coating agent, a method for promoting drought-resistant germination of crops and application of the crop seed coating agent. The crop seed coating agent comprises a water retention component, a disease-resistant component and a film-forming component, the mass ratio of the water-retaining component to the disease-resistant component to the film-forming component is 60: (2-3): (30-40); the water retention component is a chemical substance with water absorption and retention functions; the disease-resistant component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of metalaxyl-M to pullulan to polyethylene glycol 6000 is 5: 4: 1; and the film-forming component is starch and water in a mass ratio of 10: 100. The seed coating agent provided by the invention has drought-resistant, antibacterial and antiviral effects, can effectively prevent and treat crop pathogen and virus invasion, and alleviates the influence of drought conditions on crop growth.

Description

Technical Field

[0001] The present invention belongs to the technical field of seed coating agents, and specifically relates to a crop seed coating agent, a method for promoting drought-resistant germination of crops, and applications thereof. Background Art

[0002] A seed coating agent is a seed treatment technology, which has the advantages of easy operation and good control effect. Generally speaking, seed coating agents have targetability, such as for promoting root growth and germination, for preventing and controlling pathogens, or for drought resistance and water retention. Among them, preventing and controlling pathogens is the function of most seed coating agents.

[0003] According to different components, seed coating agents can be divided into chemical types, physical types, and biological types. The main components of chemical coating agents are pesticides, chemical fertilizers, hormones, etc. The main components of physical coating agents are filling particles, adhesives, etc. The components of biological coating agents are active bacteria, etc. Of course, there are also mixed coating agents in the prior art, such as compounding chemical drugs with active bacteria, compounding filling materials with hormones, compounding hormones with active bacteria, etc.

[0004] Drought conditions are water-deficient conditions, which can cause plants to lack water and affect growth and metabolism. Drought conditions are also factors that induce crop diseases. There are also many patent achievements in the research and development of drought-resistant seed coating agents in the prior art. For example, a drought-resistant seed coating agent and its preparation method disclosed in Chinese Patent CN115777704A. The coating agent is composed of a composite water-retaining agent component, an insecticide, a fungicide, and a plant growth regulator. The coating agent of this patent has strong water-retaining ability and can still maintain a high liquid absorption ratio at low temperatures. The liquid absorption ratio at 10°C is 83.5 - 84.6, the liquid absorption ratio at 15°C is 85.3 - 85.6, and the liquid absorption ratio at 20°C is 87.2 - 87.8. Another example is a drought-resistant seed coating agent and its preparation method disclosed in Chinese Patent CN115380912A. The preparation method of the drought-resistant seed coating agent includes the following steps: mixing the prepared modified high-molecular water-absorbing resin with polyaspartic acid, waxy corn starch, matrine, magnetic powder, and deionized water in a specific mass ratio, and performing ball milling after mixing evenly to obtain a drought-resistant seed coating agent. The modified high-molecular water-absorbing resin in the drought-resistant seed coating agent provided by this patent is salt-tolerant, high-temperature-resistant, and has excellent water absorption and water retention functions. When used in combination with polyaspartic acid, the drought resistance is greatly enhanced; waxy corn starch and matrine are a natural, environmentally friendly, and safe film-forming agent, insecticide, and fungicide; the addition of magnetic powder can magnetize the seeds before sowing, which plays a role in promoting seed germination.

[0005] These prior arts focus on the use of drought-resistant and antibacterial active ingredients and lack antiviral effects. Summary of the Invention

[0006] In the actual crop cultivation environment, drought and high temperature usually occur concurrently. Under such environmental conditions, viruses will multiply in large numbers, affecting the health of crops, especially viruses spread by insects, which may cause large-scale viral infection of crops. Therefore, antiviral treatment of crops is necessary. Therefore, the present invention provides a crop seed coating agent and a method and application for promoting drought-resistant germination of crops. The seed coating agent of the present invention has drought-resistant, antibacterial and antiviral effects, can effectively prevent and control the invasion of crop pathogens and viruses, and alleviate the impact of drought conditions on crop growth. The present invention also provides a crop seed germination method matched with the seed coating agent. After the method is combined with the seed coating agent of the present invention, the germination of seeds under drought and high temperature stress conditions is accelerated.

[0007] The present invention provides a crop seed coating agent, comprising: a water-retaining component, an anti-disease component and a film-forming component;

[0008] The mass ratio of the water-retaining component, the disease-resistant component and the film-forming component is 60:2-3:30-40.

[0009] The water-retaining component is a chemical substance with water-absorbing and water-retaining functions, and the water-retaining component is at least one of a water-absorbing polymer and bentonite. The water-absorbing polymer is at least one of nonionic polyacrylamide and 2-sodium acrylate homopolymer. Bentonite, nonionic polyacrylamide, and 2-sodium acrylate homopolymer can be used alone or in combination.

[0010] Non-ionic polyacrylamide is a polymer material with high water absorption and water retention. Due to its unique chemical structure, it can temporarily lock water and then slowly release it, thereby promoting seed germination.

[0011] Bentonite has water absorption, swelling, adsorption and adhesion properties, and is an ideal material for water absorption and coating film formation. Its adsorptive properties also help to mix with other coating agent components to absorb elements to promote seed germination.

[0012] 2-Sodium acrylate homopolymer has strong water absorption and excellent water retention performance.

[0013] It should be noted that the main function of the water-retaining component in the present invention is to retain water, so other water-retaining substances in the prior art are applicable to the inventive concept of the present invention, such as replacing the above-mentioned water-retaining component with the modified polymer water-absorbing resin prepared in Chinese patent CN115380912A.

[0014] In the seed coating agent of the present invention, the anti-disease components are metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of the three is 5:4:1. The English abbreviation of polyethylene glycol 6000 is PEG-6000.

[0015] Metalaxyl-M has the function of combating plant diseases.

[0016] Pullulan has the molecular formula C 20 H 36 O 16 , with the CAS number 9057-02-7, has been verified in the prior art to have the function of combating tobacco viruses, as described in Chinese Patent CN114403146A. In the actual application process, pullulan can also be used as a food additive, having the functions of thickening and stabilizing. In the present invention, it is used to make a coating agent. In addition to utilizing its above functions, it is also found that pullulan has resistance to Setaria red leaf virus, tobacco mosaic virus, and tomato mosaic virus.

[0017] Polyethylene glycol 6000 has moisture retention and adhesiveness in the present invention. What is used in the present invention is PEG-6000. Our research also shows that after being compounded with pullulan, it can enhance the antiviral effect of pullulan.

[0018] It should be noted that in the prior art, polyethylene glycol 6000 is a common reagent for crop drought stress tests, which provides a drought environment, and its addition amount in the substrate can reach more than 10%, with a relatively high dosage. In the coating agent of the present invention, polyethylene glycol 6000 is used in trace amounts to enhance the antiviral effect of pullulan and does not cause drought effects on crops.

[0019] In the seed coating agent of the present invention, the film-forming component is starch and water, and the mass ratio of the two is 10:100. The material formed after heating starch and water has an adhesive effect and is a safe film-forming component.

[0020] Preferably, the starch is corn starch, sweet potato starch, potato starch, wheat starch or mung bean starch.

[0021] Preferably, the mass ratio of the water retention component, the disease-resistant component and the film-forming component is 60:3:30.

[0022] Preferably, the mass ratio of the water retention component, the disease-resistant component and the film-forming component is 60:3:40.

[0023] A crop seed coating agent provided by the present invention is obtained by mixing the water retention component, the disease-resistant component and the film-forming component. That is, the preparation method of the crop seed coating agent is as follows:

[0024] S1, prepare the disease-resistant component

[0025] The anti-disease components are metalaxyl-M, pullulan and polyethylene glycol 6000. The solid raw materials are ground into powders of 100-325 meshes, and then the three are fully mixed in a mass ratio of 5:4:1. Note that if the particle size of the purchased raw materials is less than 325 meshes, no grinding is performed.

[0026] S2, preparation of film-forming components

[0027] The film-forming components are starch and water, which are mixed in a mass ratio of 10:100, boiled for 10-15 minutes, cooled to 40°C, and kept warm for later use;

[0028] S3, Hybrid

[0029] The water-retaining component and the film-forming component are mixed evenly, and then the disease-resistant component is added and mixed evenly to obtain a crop seed coating agent.

[0030] It should be noted that in the above-mentioned preparation method, a homogenizer or stirring means is used to achieve sufficient mixing of the materials.

[0031] It should be noted that if the raw material of the water-retaining component is solid, it is first made into a powder of 100-325 meshes, and then mixed with the film-forming component.

[0032] The present invention also provides a method for promoting drought-resistant germination of crops, which comprises mixing the crop seed coating agent with crop seeds at a mass ratio of 1:10-12, coating the seeds with a seed coating machine, and then drying the coated seeds and sowing them.

[0033] Preferably, the crop is millet, wheat, corn or rice. The above method for promoting drought-resistant germination of crops has a significant effect on millet, wheat, corn or rice.

[0034] The present invention also provides an application of a crop seed coating agent, wherein the seed coating agent is used to improve the germination rate, pathogen resistance and virus resistance of crop seeds under drought conditions.

[0035] For example, the pathogen resistance refers to the inhibition of millet white spot pathogens and millet smut pathogens;

[0036] Antiviral property refers to the inhibition of millet red leaf virus, tobacco mosaic virus, and tomato mosaic virus.

[0037] Compared with the prior art, the present invention has the following beneficial effects:

[0038] The present invention provides a crop seed coating agent, which has the effects of drought resistance, antibacterial and antiviral, can effectively prevent and control the invasion of crop pathogens and viruses, and alleviate the impact of drought conditions on crop growth. The present invention also provides a supporting method for crop seed germination, which is combined with the seed coating agent of the present invention to accelerate the germination of seeds under drought and high temperature stress conditions.

[0039] In the present invention, metalaxyl - M has an antibacterial effect, pullulan has resistance to Setaria red leaf virus, tobacco mosaic virus, and tomato mosaic virus. After polyethylene glycol 6000 is compounded with pullulan, the antiviral effect of pullulan can be enhanced. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] Figure 1 is the technical roadmap of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0041] In order to enable those skilled in the art to better understand and implement the technical solutions of the present invention, the present invention will be further described below in conjunction with specific embodiments and drawings.

[0042] In the description of the present invention, unless otherwise specified, the reagents used are commercially available, and the methods used are conventional techniques in the art.

[0043] In the embodiments and tests of the present invention, metalaxyl - M is purchased from Quanzhou Shuoxin Biotechnology Co., Ltd., with an active substance content of 98%; non - ionic polyacrylamide is purchased from Gongyi Fuda Water Purification Materials Co., Ltd., with a solid content of ≥33% and a molecular weight of 3 million - 12 million; the pullulan used is purchased from Shaanxi Chenming Biotechnology Co., Ltd., with an active ingredient content of 99%; the food - grade corn starch used is purchased from Shandong Gushuo Biotechnology Co., Ltd., with an active ingredient content of 99%; the water used is deionized water to exclude the interference of ions in tap water on the antiviral effect; poly(acrylic acid - sodium) with a pH of 6.5 and a molecular weight of <10000 is purchased from Hebei Hengju Chemical Co., Ltd., with an EINECS registration number of 999 - 999 - 2 and a product number of water - absorbing resin 001.

[0044] In the embodiments and tests of the present invention, solid raw materials are ground into powders with a mesh size of 325, and then used. If the particle size of the purchased raw materials is less than 325 mesh, no grinding treatment is required, but it is necessary to ensure that the raw material sources and batches used in different embodiments and different control examples are the same to exclude the interference of different batches of raw materials on the experimental results. Figure 1 is the technical roadmap of the present invention.

[0045] Example 1

[0046] A crop seed coating agent, comprising: a water - retaining component, a disease - resistant component, and a film - forming component;

[0047] The mass ratio of the water-retaining component, the disease-resistant component and the film-forming component is 60:2:30;

[0048] The disease-resistant component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of the three is 5:4:1;

[0049] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0050] The water-retaining component is non-ionic polyacrylamide.

[0051] The preparation method of the crop seed coating agent is as follows:

[0052] S1. Prepare the disease-resistant component

[0053] The disease-resistant component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the three are fully mixed according to the mass ratio of 5:4:1;

[0054] S2. Prepare the film-forming component

[0055] The film-forming component is starch and water, and the two are mixed according to the mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0056] S3. Mixing

[0057] Mix the water-retaining component and the film-forming component evenly, then add the disease-resistant component and mix evenly to obtain the crop seed coating agent.

[0058] Example 2

[0059] A crop seed coating agent, comprising: a water-retaining component, a disease-resistant component and a film-forming component;

[0060] The mass ratio of the water-retaining component, the disease-resistant component and the film-forming component is 60:2:40;

[0061] The disease-resistant component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of the three is 5:4:1;

[0062] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0063] The water-retaining component is non-ionic polyacrylamide.

[0064] The preparation method of the crop seed coating agent is as follows:

[0065] S1. Prepare the disease-resistant component

[0066] The anti-disease component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the three are fully mixed according to the mass ratio of 5:4:1;

[0067] S2. Prepare the film-forming component

[0068] The film-forming component is starch and water, and the two are mixed according to the mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0069] S3. Mix

[0070] Mix the water-retaining component and the film-forming component evenly, and then add the anti-disease component and mix evenly to obtain the crop seed coating agent.

[0071] Example 3

[0072] A crop seed coating agent, comprising: a water-retaining component, an anti-disease component and a film-forming component;

[0073] The mass ratio of the water-retaining component, the anti-disease component and the film-forming component is 60:3:30;

[0074] The anti-disease component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of the three is 5:4:1;

[0075] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0076] The water-retaining component is non-ionic polyacrylamide.

[0077] The preparation method of the crop seed coating agent is as follows:

[0078] S1. Prepare the anti-disease component

[0079] The anti-disease component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the three are fully mixed according to the mass ratio of 5:4:1;

[0080] S2. Prepare the film-forming component

[0081] The film-forming component is starch and water, and the two are mixed according to the mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0082] S3. Mix

[0083] Mix the water-retaining component and the film-forming component evenly, and then add the anti-disease component and mix evenly to obtain the crop seed coating agent.

[0084] Example 4

[0085] A crop seed coating agent, comprising: a water-retaining component, an anti-disease component and a film-forming component;

[0086] The mass ratio of the water-retaining component, the disease-resistant component and the film-forming component is 60:3:40;

[0087] The disease-resistant component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of the three is 5:4:1;

[0088] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0089] The water-retaining component is non-ionic polyacrylamide.

[0090] The preparation method of the crop seed coating agent is as follows:

[0091] S1. Prepare the disease-resistant component

[0092] The disease-resistant component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the three are fully mixed according to the mass ratio of 5:4:1;

[0093] S2. Prepare the film-forming component

[0094] The film-forming component is starch and water, and the two are mixed according to the mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0095] S3. Mix

[0096] Mix the water-retaining component and the film-forming component evenly, then add the disease-resistant component and mix evenly to obtain the crop seed coating agent.

[0097] Example 5

[0098] A crop seed coating agent, comprising: a water-retaining component, a disease-resistant component and a film-forming component;

[0099] The mass ratio of the water-retaining component, the disease-resistant component and the film-forming component is 60:2:30;

[0100] The disease-resistant component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of the three is 5:4:1;

[0101] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0102] The water-retaining component is 2-acrylate homopolymer.

[0103] The preparation method of the crop seed coating agent is as follows:

[0104] S1. Prepare the disease-resistant component

[0105] The anti-disease component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the three are fully mixed according to the mass ratio of 5:4:1;

[0106] S2. Prepare the film-forming component

[0107] The film-forming component is starch and water, and the two are mixed according to the mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0108] S3. Mix

[0109] Mix the water-retaining component and the film-forming component evenly, and then add the anti-disease component and mix evenly to obtain the crop seed coating agent.

[0110] Control Example 1: When the anti-disease component is metalaxyl-M, the crop seed coating agent includes: a water-retaining component, an anti-disease component and a film-forming component;

[0111] The mass ratio of the water-retaining component, the anti-disease component and the film-forming component is 60:2:30;

[0112] The anti-disease component is metalaxyl-M;

[0113] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0114] The water-retaining component is non-ionic polyacrylamide.

[0115] The preparation method of the crop seed coating agent is as follows:

[0116] S(1). Prepare the film-forming component

[0117] The film-forming component is starch and water, and the two are mixed according to the mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0118] S(2). Mix

[0119] Mix the water-retaining component and the film-forming component evenly, and then add the anti-disease component and mix evenly to obtain the crop seed coating agent.

[0120] Control Example 2: When the anti-disease component is pullulan, the crop seed coating agent includes: a water-retaining component, an anti-disease component and a film-forming component;

[0121] The mass ratio of the water-retaining component, the anti-disease component and the film-forming component is 60:2:30;

[0122] The anti-disease component is pullulan;

[0123] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0124] The water retention component is non-ionic polyacrylamide.

[0125] The preparation method of the crop seed coating agent is as follows:

[0126] S(1), preparing the film-forming component

[0127] The film-forming component is starch and water, and the two are mixed in a mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0128] S(2), mixing

[0129] The water retention component and the film-forming component are mixed evenly, and then the disease-resistant component is added and mixed evenly to obtain the crop seed coating agent.

[0130] Control Example 3: When the disease-resistant component is polyethylene glycol 6000, the crop seed coating agent includes: a water retention component, a disease-resistant component, and a film-forming component;

[0131] The mass ratio of the water retention component, the disease-resistant component, and the film-forming component is 60:2:30;

[0132] The disease-resistant component is polyethylene glycol 6000;

[0133] The film-forming component is starch and water, and the mass ratio of the two is 10:100.

[0134] The water retention component is non-ionic polyacrylamide.

[0135] The preparation method of the crop seed coating agent is as follows:

[0136] S(1), preparing the film-forming component

[0137] The film-forming component is starch and water, and the two are mixed in a mass ratio of 10:100, boiled for 10 min, cooled to 40 °C, and kept warm for standby;

[0138] S(2), mixing

[0139] The water retention component and the film-forming component are mixed evenly, and then the disease-resistant component is added and mixed evenly to obtain the crop seed coating agent.

[0140] Effect verification 1: Antibacterial test

[0141] 1. Test strains: Sclerospora graminicola, the pathogen of downy mildew of foxtail millet, can infect at all stages of seed germination and maturity, affecting the growth of foxtail millet. Since this invention mainly aims to improve the drought-resistant germination performance of seeds, this strain was tested. Sclerospora graminicola is recorded in the prior art "Chang Guorong, Li Renjian, Zhang Qi, etc. Identification of co-expressed genes responsive to Sclerospora graminicola stress by endogenous abscisic acid in foxtail millet using WGCNA [J]. Scientia Agricultura Sinica, 2020, No. 16, 3280 - 3293".

[0142] The pathogen of smut, Ustilago virens, is recorded in "Guo Lin, Ustilago virens in Arshan, Inner Mongolia [J]. Journal of Jilin Agricultural University, 1998, Supplement 1, 213 - 213". Smut of foxtail millet mainly infects the grains of the ear, which is also a factor affecting seed quality.

[0143] 2. Test method: Mix 100 mL of the bacterial solution with 0.1 g of the coating agent, let it stand for 1 h, and respectively count the number of viable bacteria in the bacterial solution before adding the coating agent and the number of viable bacteria after standing for 2 h.

[0144] Calculate the antibacterial rate, and the antibacterial rate = 100% × (the number of viable bacteria in the bacterial solution before adding the coating agent - the number of viable bacteria after standing for 1 h) / the number of viable bacteria in the bacterial solution before adding the coating agent.

[0145] Each test is done in triplicate, and the average value is taken. Using the one without adding any coating agent as the negative control.

[0146] 3. Test results

[0147] The results are shown in Table 1 below. Table 1 results show that Examples 1 - 4 explore the influence of the mass ratios of different water-retaining components, disease-resistant components, and film-forming components on the antibacterial effect.

[0148] The antibacterial rates of Control Examples 1 - 3 are not as good as those of Examples 1 - 5.

[0149] Table 1 Antibacterial rate results

[0150]

[0151] Effect verification two: Antiviral test

[0152] 1. Test viruses: Barley yellow dwarf virus (BYDV), Tobacco mosaic virus (TMV), and Tomato mosaic virus (ToMV). Among them, the abbreviation of Barley yellow dwarf virus is BYDV virus, and the abbreviation of Tobacco mosaic virus is TMV virus.

[0153] 2. Test method: The half-leaf lesion method was used for determination. The lesion host was millet leaves, which were divided into two halves. One half was used for treatment, and the other half was untreated as a healthy control. The specific operation method referred to the test method in 2.2 of "Yin Peijun. Study on the Activity of Plant-derived Antiviral Agent VFB against Tobacco Mosaic Virus (TMV) [D]. Northwest A&F University, 2005". The concentration of the virus inoculation solution used was 2 g / 100 mL. Among them, the virus inoculation solution was used as the positive control, and the one treated with the drug was the test group. The drug treatment refers to mixing the coating agent and the virus inoculation solution in a mass ratio of 1:1 and then inoculating it on the leaves.

[0154] The antiviral effect was divided according to the final lesion area, and the classification criteria were as follows:

[0155] +++: Indicates a significant virus inhibition effect. The lesion area is 30% or less of the area of the healthy control, including 30%;

[0156] ++: Indicates a general virus inhibition effect. The lesion area is 30% - 60% of the area of the healthy control, excluding 30% and 60%;

[0157] +: Indicates a virus inhibition effect. The lesion area is 60% - 90% of the area of the healthy control, including 60% and 90%;

[0158] -: Indicates no virus inhibition effect. The lesion area is more than 90% of the area of the healthy control, excluding 90%.

[0159] 3. Test results

[0160] The results are shown in Table 2 below. Table 2 results show that the coating agents of each example have antiviral effects.

[0161] Table 2 Antiviral test results

[0162]

[0163] Effect verification three: Water retention capacity test

[0164] 1. Test method: Weigh 0.5 g of the coating agent and place it in a beaker. Add 2000 ml of distilled water, let it stand for 24 h, then filter it with a 250-mesh gauze and let it stand for about 30 min. Weigh the mass of the gelled coating agent and calculate the liquid absorption ratio Q = (m 1 -m 0 ) / m 0 , where: m 0 is the mass of the coating agent before water absorption, m 1It is the quality of the coating agent gel after being filtered by gauze after water absorption. This method refers to Chinese Patent CN115777704A. According to this method, the liquid absorption ratio of the coating agent is tested at 10°C, 25°C, and 40°C respectively. Each test is done in triplicate and the average value is taken. The results are shown in Table 3.

[0165] 3. Test Results

[0166] The results are shown in Table 3 below. The results in Table 3 show that each example has a good water absorption and water retention effect.

[0167] Table 3 Test Results of Water Retention Ability

[0168] Coating agent Liquid absorption ratio at 10°C Liquid absorption ratio at 25°C Liquid absorption ratio at 40°C Example 1 37.04±0.16 37.26±0.09 39.83±0.07 Example 2 36.05±0.08 37.17±0.08 39.11±0.11 Example 3 35.97±0.21 36.88±0.11 39.40±0.11 Example 4 34.61±0.15 35.70±0.12 39.55±0.09 Example 5 67.59±0.10 68.94±0.23 70.33±0.22 Control Example 1 36.01±0.09 36.94±0.07 38.54±0.14 Control Example 2 36.53±0.11 36.61±0.02 38.60±0.04 Control Example 3 36.11±0.15 36.90±0.05 38.53±0.11

[0169] Effect Verification Four: Drought Resistance Germination Test

[0170] 1. Test Method:

[0171] The crop seed coating agent and crop seeds are mixed at a mass ratio of 1:10 for coating treatment, and then the coated seeds are dried.

[0172] Lay a thick sponge under a single layer of filter paper and place it flat in a 90mm petri dish. After absorbing enough liquid matrix, randomly select 60 coated seeds from each group and place them evenly on it for cultivation at room temperature. The liquid matrix is an aqueous solution of 16% mass fraction PEG-6000, providing drought stress conditions.

[0173] The germination rate is measured 5 days after germination. Germination rate = (100% × number of germinated seeds / number of seeds for inspection). This germination rate measurement method refers to Chinese Patent CN115380912A, and the test results are shown in Table 4.

[0174] 2. Test Results

[0175] The results are shown in Table 4 below. The results in Table 4 show that the germination rates of Examples 1 to 5 are higher than those of Control Examples 1 to 3, indicating that the coating agent provided by the present invention has a good drought resistance germination effect.

[0176] Table 4 Test Results of Drought Resistance Germination

[0177]

[0178]

[0179] It should be noted that when the present invention involves a numerical range, it should be understood that any value between the two endpoints of each numerical range, as well as the two endpoints themselves, can be selected. Since the steps and methods adopted are the same as those in the embodiments, for the sake of avoiding redundancy, the present invention describes the preferred embodiments. Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications to these embodiments once they know the basic creative concept. Therefore, the appended claims are intended to be construed as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0180] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these changes and modifications.

Claims

1. A crop seed coating agent, characterized in that: include: Water-retaining components, disease-resistant components, and film-forming components; The mass ratio of water-retaining component, disease-resistant component and film-forming component is 60:2-3:30-40; The water-retaining component is a chemical substance having the function of absorbing and retaining water; The anti-disease component is metalaxyl-M, pullulan and polyethylene glycol 6000, and the mass ratio of the three is 5:4:1; The film-forming components are starch and water, and the mass ratio of the two is 10:

100.

2. The crop seed coating agent according to claim 1, characterized in that The water-retaining component is at least one of a high molecular polymer and bentonite having water absorption property.

3. The crop seed coating agent according to claim 2, characterized in that The water-absorbing high molecular polymer is at least one of nonionic polyacrylamide and 2-sodium acrylate homopolymer.

4. The crop seed coating agent according to claim 1, characterized in that The crop seed coating agent is obtained by mixing a water-retaining component, an anti-disease component and a film-forming component.

5. A method for promoting drought-resistant germination of crops, characterized in that: The crop seed coating agent according to claim 1 is mixed with crop seeds in a mass ratio of 1:10-12, and then the coated seeds are dried and sown.

6. The method for promoting drought-resistant germination of crops according to claim 5, characterized in that: The crop is millet, wheat, corn or rice.

7. The use of the crop seed coating agent according to claim 1, characterized in that: The seed coating agent is used for improving the germination rate, pathogen resistance and virus resistance of crop seeds under drought conditions.

8. The use of the crop seed coating agent according to claim 7, characterized in that: The pathogen resistance refers to the inhibition of millet white spot disease pathogens and millet smut pathogens.

9. The use of the crop seed coating agent according to claim 7, characterized in that: The antiviral property refers to the inhibition of millet red leaf virus, tobacco mosaic virus and tomato mosaic virus.

Images