Iron-based biological leaf surface protective agent as well as preparation method and application thereof

Abstract

The invention discloses an iron-based biological leaf surface protective agent as well as a preparation method and application thereof, and the iron-based biological leaf surface protective agent is prepared from the following raw materials in parts by weight: 2 to 20 parts of EDTA-Fe, 50 to 200 parts of urea, 30 to 100 parts of monopotassium phosphate, 10 to 20 parts of boric acid, 20 to 40 parts of magnesium sulfate, 200 to 400 parts of nano silicon dioxide, 30 to 100 parts of a biological source synergist and 11 to 40 parts of an auxiliary agent, the biological source synergist comprises a Trichoderma asperellum TaLY2 fermentation broth, the Trichoderma asperellum TaLY2 fermentation broth is obtained by inoculating a Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum Trichoderma asperellum The iron-based biological leaf surface protective agent disclosed by the invention solves the problems of short effective period, high application frequency and single function of a traditional leaf surface iron fertilizer, has a good prevention and treatment effect on the curvularia leaf spot of corn, further promotes the growth of the corn and improves the yield of the corn.

Claims

1. An iron-based biological leaf protective agent, characterized in that: The invention comprises the following raw materials in parts by weight: 2-20 parts by weight of EDTA-Fe, 50-200 parts by weight of urea, 30-100 parts by weight of potassium dihydrogen phosphate, 10-20 parts by weight of boric acid, 20-40 parts by weight of magnesium sulfate, 200-400 parts by weight of nano silicon dioxide, 30-100 parts by weight of a biogenic synergist and 11-40 parts by weight of an adjuvant. The biogenic synergist comprises a fermentation liquid of Trichoderma asperellum TaLY2, which is obtained by inoculating a seed liquid of Trichoderma asperellum TaLY2 into a fermentation medium for fermentation and culturing, and the Trichoderma asperellum TaLY2 has been deposited in the General Microbiological Center of China National Committee for Microbiological Culture Collection, with a deposit number of CGMCC NO.40749.

2. The iron-based biological leaf protective agent according to claim 1, characterized in that: The auxiliary agent comprises 10-30 parts by weight of a preservative and 1-10 parts by weight of a surfactant.

3. The iron-based biological leaf protective agent according to claim 2, characterized in that: The preservative is xanthan gum, and the surfactant is sodium dodecyl sulfate.

4. The iron-based biological leaf protective agent according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 15 parts by weight of EDTA-Fe, 100 parts by weight of urea, 50 parts by weight of potassium dihydrogen phosphate, 10 parts by weight of boric acid, 20 parts by weight of magnesium sulfate, 250 parts by weight of nano silicon dioxide, 50 parts by weight of Trichoderma aspergillus TaLY2 fermentation liquid and 30 parts by weight of auxiliary agent.

5. A method for preparing the iron-based biological foliar protective agent according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1, preparing Trichoderma aspergillus TaLY2 fermentation broth; S2, mixing the fermentation broth of Trichoderma aspergillus TaLY2 with EDTA-Fe, urea, potassium dihydrogen phosphate, boric acid and magnesium sulfate to obtain a mixed solution; S3. Add nano silicon dioxide to the mixed solution prepared in step S3, disperse by ultrasonic, add an auxiliary agent, mix well, and obtain an iron-based biological foliar protective agent.

6. The method for preparing the iron-based biological leaf protective agent according to claim 5, characterized in that: The step S1 is to prepare the fermentation broth of Trichoderma aspergillus TaLY2, which specifically comprises the following steps: S11, strain culture: inoculating the activated Trichoderma asperellum TaLY2 into a liquid culture medium for culture, to obtain a Trichoderma asperellum TaLY2 seed solution; S12, preparing fermentation medium; S13, fermentation culture: Trichoderma asperellum TaLY2 seed liquid is inoculated into the fermentation medium at a ratio of 2-3 v / v%. After fermentation culture, the medium is sterilized and centrifuged, and the supernatant is collected to obtain Trichoderma asperellum TaLY2 fermentation liquid.

7. The method for preparing the iron-based biological leaf protective agent according to claim 6, characterized in that: In step S12, the fermentation medium includes the following components: Sucrose 1-3wt%, peptone 0.5-1.5wt%, ammonium sulfate 0.5-1.5wt%, potassium chloride 1-2wt%, the balance is distilled water, initial pH=5-6.

8. The method for preparing the iron-based biological leaf protective agent according to claim 6 or 7, characterized in that: The conditions for fermentation culture in step S13 include: When the fermentation time is from 0 to 24 h, the fermentation temperature is 28 °C, the rotation speed is 200 rpm, the aeration rate is 0.6 vvm, and the pH is natural; when the fermentation time is from 24 to 96 h, the fermentation temperature is 28 °C, the rotation speed is 150 rpm, the aeration rate is 0.8 vvm, and the pH is natural; when the fermentation time is more than 96 h, the fermentation temperature is 28 °C, the rotation speed is 120 rpm, the aeration rate is 0.6 vvm, and the fermentation is stopped when the pH value reaches 5.0 < pH ≤ 5.5, and then heated to 65 - 70 °C and maintained for 15 minutes.

9. Application of the iron-based biological foliar protectant according to any one of claims 1 - 4 or the iron-based biological foliar protectant prepared by the preparation method according to any one of claims 5 - 8 in controlling Curvularia leaf spot of maize.

10. The use according to claim 9, characterized in that: The application method of the iron-based biological foliar protective agent is as follows: the dosage of the iron-based biological foliar protective agent is 100-300 mL / 667 m 2 , dilute 100-300 times and spray on corn plants during the corn tassel stage, apply 1-2 times, with an interval of 7-10 days between two applications.

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