Nano slow-release oil-based composition as well as preparation method, feeding method and application thereof

Abstract

The invention provides a nano slow-release oil-based composition as well as a preparation method, a feeding method and application thereof, and relates to the technical field of animal health-care products. The nano slow-release oil-based composition is a composition which is formed by capturing and clathrating nano liquid oil drops dispersed in a three-dimensional net-shaped structure formed by a gelling agent, yeast, protein and phospholipid in a plant source oil substance, wherein the three-dimensional net-shaped structure is formed by the gelling agent, the yeast, the protein and the phospholipid in the plant source oil substance; wherein the nanometer liquid oil drops are nanometer liquid oil drops formed by plant source oil substances. According to the invention, a three-dimensional network structure is formed by the gelling agent, the yeast, the protein and phospholipid in the plant source oil substances to effectively wrap the nano liquid oil drops, so that the stability and the nutritional value of the product are enhanced, the palatability is improved, the digestion burden of pets is relieved, and the influence on the environment is reduced. In addition, dependence of pets on additives is reduced, safety and satiety of the product are improved, weight management is facilitated, and the production process is green and environmentally friendly.

Description

Technical Field

[0001] The present invention relates to the technical field of animal health products, and in particular, to a nano-sustained release oil-based composition, a preparation method, a feeding method, and an application thereof. Background Art

[0002] Animal health and welfare are topics that have received increasing attention in modern agriculture and household breeding, including not only livestock and poultry but also companion animals such as pets. With social progress and the improvement of people's living standards, pets such as dogs, cats, and other small pets have become an indispensable part of many families. The role of pets has gradually shifted from functional to emotional companionship, and people's care for pets has changed from basic feeding to comprehensive attention to their health and appearance. In this trend, the health and beauty needs of pets have given rise to the rapid development of animal health product technology, and various products aimed at improving the quality of life of pets have emerged on the market.

[0003] Among many animal health products, pet hair beauty and care products have received special attention because they are directly related to the appearance and health of pets. Currently, such products mostly appear in the form of compound nutrients, with main ingredients including lecithin, fish oil, etc., supplemented by nutrients such as vitamins, minerals, and amino acids. The core function of these products is to improve the coat quality of pets and enhance their appearance by supplementing key nutrients such as Omega-3 polyunsaturated fatty acids.

[0004] However, there are some significant problems with pet hair beauty and care products mainly composed of fish oil in the prior art. Although Omega-3 polyunsaturated fatty acids in fish oil are beneficial to the health of pet coats, their unsaturated double bonds make them extremely prone to oxidation, especially during processing and storage, and are easily affected by environmental factors such as light, heat, and oxygen, resulting in a decrease in nutritional value and the generation of peculiar smells. In addition, in order to cover up the fishy smell of fish oil and the peculiar smell generated by oxidation, some products add attractants and flavor masking agents, which may not only cause pets to be picky eaters and suffer from malnutrition but also impose an additional burden on the digestive system of pets. At the same time, excessive intake of EPA in fish oil in the short term may affect the blood coagulation function of pets, leading to health risks such as prolonged bleeding time.

[0005] In summary, the main defects of existing pet hair beauty and care products include: easy oxidation leading to a decrease in product stability and nutritional value; the addition of attractants and flavor masking agents may cause health problems for pets; the limited fish oil resources and the environmental and health risks they bring; and the possible negative impact of EPA on the blood coagulation function of pets. These problems limit the effectiveness and application of existing products, indicating an urgent need for new, safer, and more effective pet hair beauty and care products.

[0006] In view of this, the present invention is specifically proposed. Summary of the Invention

[0007] The object of the present invention is to provide a nano slow-release oil-based composition and a preparation method, feeding method and application thereof, wherein the nano slow-release oil-based composition improves stability and nutritional value, improves palatability, reduces digestive burden, reduces environmental risks, reduces dependence on additives, and helps pet weight management.

[0008] In order to achieve the above-mentioned purpose of the present invention, the following technical solutions are particularly adopted:

[0009] In a first aspect, the present invention provides a nano slow-release oil-based composition, wherein the nano slow-release oil-based composition is a composition consisting of a three-dimensional network structure formed by a gelling agent, yeast, protein, and phospholipids in plant-based oil substances, and captures and encloses nano liquid oil droplets dispersed in the three-dimensional network structure; wherein the nano liquid oil droplets are nano liquid oil droplets formed by plant-based oil substances. In an optional embodiment, the plant-based oil substances include algae oil and linseed oil;

[0010] In an optional embodiment, the nano slow-release oil-based composition includes the following components in parts by weight:

[0011] 30 to 70 parts of algae oil;

[0012] 20-60 parts of linseed oil;

[0013] 5 to 20 servings of protein;

[0014] 0.6 to 10 parts of gelling agent;

[0015] 0.1 to 3 parts of yeast;

[0016] In an alternative embodiment, the protein is whey protein;

[0017] In an alternative embodiment, the yeast is Saccharomyces cerevisiae.

[0018] In an optional embodiment, the algae oil includes at least one of DHA algae oil, EPA algae oil and Haematococcus pluvialis oil;

[0019] In an optional embodiment, the algae source range of the DHA algae oil includes at least one of Schizochytrium, Okenichthys and Crypthecodinium cohnii;

[0020] In an alternative embodiment, the algae source range of the EPA algae oil includes Nannochloropsis spp.

[0021] In an optional embodiment, the weight proportion of DHA algae oil in the algae oil is 15 to 40 parts; the weight proportion of EPA algae oil is 10 to 35 parts; and the weight proportion of Haematococcus pluvialis oil is 0.2 to 3.5 parts.

[0022] In an alternative embodiment, the whey protein comprises at least one of whey protein isolate, whey protein concentrate, hydrolyzed whey protein, and demineralized whey powder.

[0023] In an alternative embodiment, the gelling agent comprises an aqueous gelling agent and an oil-phase gelling agent.

[0024] In an alternative embodiment, the aqueous gelling agent comprises at least one of gelatin, xanthan gum, sodium alginate, chitosan, galactomannan, pectin, and gellan gum;

[0025] In an alternative embodiment, the oil-phase gelling agent comprises at least one of ethyl cellulose, hydroxypropyl methylcellulose, and candelilla wax;

[0026] In an alternative embodiment, the weight parts of the aqueous gelling agent are 0.1 part to 5 parts; the weight parts of the oil-phase gelling agent are 0.5 part to 8 parts.

[0027] In a second aspect, the present invention provides a method for preparing a nano-sustained release oil-based composition as described in any one of the foregoing embodiments, comprising:

[0028] S1, subjecting the protein, the aqueous gelling agent, and water to a first stirring and mixing treatment under a first heating condition to obtain an aqueous mixture;

[0029] S2, subjecting linseed oil and EPA algal oil in algal oil to a second stirring and mixing treatment under a second heating condition to obtain an oil-phase mixture;

[0030] S3, adding the oil-phase gelling agent and yeast to the oil-phase mixture, and performing a third stirring and mixing treatment under the second heating condition to obtain an oil-based condensate;

[0031] S4, cooling the oil-based condensate, and adding the DHA algal oil and Haematococcus pluvialis oil in the algal oil to the aqueous mixture respectively to obtain an oil-water condensate; and, performing a third stirring and mixing treatment, an emulsification treatment, and a high-pressure homogenization treatment on the oil-water condensate, so that the protein, phospholipids in the EPA algal oil, the aqueous gelling agent, and the oil-phase gelling agent in the oil-water condensate form a three-dimensional network structure, and capture and encapsulate the dispersed nano liquid oil droplets to form a composite oil-based condensate-emulsion system;

[0032] Step S5, drying the composite oil-based condensate-emulsion system to obtain a nano-sustained release oil-based composition;

[0033] In an alternative embodiment, the temperature of the first heating condition is not higher than 45°C;

[0034] In an alternative embodiment, the rotation speed of the first stirring and mixing treatment is 100 r / min to 2000 r / min;

[0035] In an alternative embodiment, the time of the first stirring and mixing treatment is 5 minutes to 30 minutes;

[0036] In an alternative embodiment, the temperature of the second heating condition is 60 °C to 90 °C;

[0037] In an alternative embodiment, the stirring time of the second stirring and mixing treatment is 15 minutes to 30 minutes;

[0038] In an alternative embodiment, the stirring rate of the second stirring and mixing treatment is 60 r / min to 500 r / min;

[0039] In an alternative embodiment, the stirring rate of the third stirring and mixing treatment is 300 r / min to 3000 r / min;

[0040] In an alternative embodiment, the stirring time of the third stirring and mixing treatment is 5 minutes to 30 minutes;

[0041] In an alternative embodiment, in step S5, the drying treatment includes heating vacuum drying or freeze vacuum drying;

[0042] In an alternative embodiment, in step S5, the water content of the nano-sustained release oil-based composition is controlled not to exceed 0.5% by the drying treatment.

[0043] In an alternative embodiment, in step S1, the weight parts of the protein, the aqueous gelling agent and water are:

[0044] Protein 5 parts to 20 parts;

[0045] Aqueous gelling agent 0.1 part to 5 parts;

[0046] Water 350 parts to 850 parts.

[0047] In an alternative embodiment, in step S2, the weight parts of linseed oil and EPA algal oil are:

[0048] Linseed oil 20 parts to 60 parts;

[0049] EPA algal oil 10 parts to 35 parts.

[0050] In an alternative embodiment, in step S4, the oil-based condensate is cooled and then the DHA algal oil and Haematococcus pluvialis oil in the algal oil are separately added to the aqueous phase mixture to obtain an oil-water condensate; and the oil-water condensate is subjected to a third stirring and mixing treatment, an emulsification treatment, and a high-pressure homogenization treatment, so that proteins, phospholipids in EPA algal oil, the aqueous phase gelling agent, and the oil phase gelling agent form a three-dimensional network structure and capture and encapsulate the dispersed nano liquid oil droplets to form a composite oil-based condensate-emulsion system, including:

[0051] Cool the oil-based condensate to a temperature below 70 °C;

[0052] Take the DHA algal oil and Haematococcus pluvialis oil, add them to the oil-based condensate after the cooling treatment, and perform the second stirring and mixing treatment to obtain an oil-based condensate-DHA algal oil mixture;

[0053] Add the oil-based condensate-DHA algal oil mixture to the aqueous phase mixture to obtain the oil-water condensate;

[0054] Perform a fourth stirring and mixing treatment, an emulsification treatment, and a high-pressure homogenization treatment on the oil-water condensate in sequence, so that proteins, phospholipids in EPA algal oil, the aqueous phase gelling agent, and the oil phase gelling agent form a three-dimensional network structure and capture and encapsulate the dispersed nano liquid oil droplets to form the composite oil-based condensate-emulsion system;

[0055] In an alternative embodiment, the weight ratio of the added DHA algal oil and Haematococcus pluvialis oil to the oil-based condensate is: (10 - 45):(0.2 - 3.5):(30 - 80);

[0056] In an alternative embodiment, the ratio of the oil-based condensate-DHA algal oil mixture to the aqueous phase mixture is 1:(5 - 10).

[0057] In an alternative embodiment, in step S3, the weight ratio of the added oil phase gelling agent and yeast to the oil phase mixture is (0.5 - 8):(0.1 - 3):(30 - 80).

[0058] Thirdly, the present invention provides a feeding method for the nano-sustained-release oil-based composition as described in the foregoing embodiments, or the nano-sustained-release oil-based composition prepared by the preparation method of the nano-sustained-release oil-based composition as described in the foregoing embodiments, and the feeding method includes at least one of the following methods:

[0059] A. Directly feed the nano-sustained-release oil-based composition;

[0060] B. Mix the nano - sustained - release oil - based composition with animal feed and then feed;

[0061] C. Cover the surface of the animal feed with the nano - sustained - release oil - based composition and then feed;

[0062] Wherein, the feed includes food and / or medicine; the food includes at least one of granular dry food, wet food, and freeze - dried meat particles.

[0063] In a fourth aspect, the present invention provides a nano - sustained - release oil - based composition as described in the foregoing embodiments, or a nano - sustained - release oil - based composition prepared by the preparation method of the nano - sustained - release oil - based composition as described in the foregoing embodiments, for use in the preparation of animal health products, where the animal health products include at least one of hair - beautifying products and weight - loss products.

[0064] This application provides a nano - sustained - release oil - based composition, its preparation method, feeding method, and application. The nano - sustained - release oil - based composition improves the stability and shelf life of the product, reduces the oxidation risk by reducing the contact of oil droplets with air, light, and heat; improves palatability, avoids bad odors caused by raw materials, and improves the acceptance of pets for the product; improves nutritional value and bioavailability, especially enhances the absorption rate of Omega - 3 polyunsaturated fatty acids through phospholipids in the oil - based composition; reduces the burden on the digestive system of pets, making nano - sized oil droplets easier to digest and absorb; reduces the risk of pets becoming dependent on additives and being picky eaters because the product does not rely on additional attractants and flavor - masking agents; reduces environmental pollution and health risks, uses sustainable - source plant - derived oil substances such as microalgae oil, and reduces the dependence on deep - sea fish resources; controls the release of nutrients, improves the safety of the product, especially reduces the impact on the blood - clotting function of pets due to excessive intake in the short term; enhances satiety, helps control the weight of obese pets because the nano - sustained - release oil - based composition prolongs the gastric emptying time; and its production process is environmentally friendly and conforms to the development trend of green environmental protection. These comprehensive effects make this technology have significant potential and advantages in the field of pet health. Description of the Drawings

[0065] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for use in the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0066] Figure 1 It is a schematic diagram of the structure and morphology of substances in the nano - sustained - release oil - based composition in the embodiments of this application;

[0067] Figure 2 This is a schematic flow chart of the preparation method of the nano-sustained release oil-based composition in the embodiments of the present application.

[0068] Explanation of reference numerals:

[0069] 100, nano-sustained release oil-based composition; 1, three-dimensional network structure; 2, nano-liquid oil droplets. Detailed implementation manners

[0070] The following will describe the implementation manners of the present invention in detail in conjunction with embodiments. However, those skilled in the art will understand that the following embodiments are only used to illustrate the present invention and should not be regarded as limiting the scope of the present invention. For those not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. Those reagents or instruments not specified by the manufacturer can be obtained as conventional products through commercial purchase.

[0071] Reference Figure 1 , in the embodiments of the present application, a nano-sustained release oil-based composition is provided. The nano-sustained release oil-based composition is a composition formed by a three-dimensional network structure formed by a gelling agent, yeast, protein, and phospholipids in vegetable oil substances, which captures and encapsulates nano-liquid oil droplets dispersed in the three-dimensional network structure; wherein, the nano-liquid oil droplets are nano-liquid oil droplets formed by vegetable oil substances.

[0072] The above-mentioned oil-based composition is a lipid mixture of the nano-sustained release oil-based composition with thermoreversibility (heating melts, cooling solidifies) and plasticity, and has both the functions of liquid oil and the stability of solid state.

[0073] In the embodiments of the present application, a three-dimensional network structure is formed by a gelling agent, yeast, protein, and phospholipids in vegetable oil substances, and the oil droplets are captured in the three-dimensional network structure to inhibit the flow of liquid oil, so that it has certain functional structural characteristics and improves the stability of liquid oil.

[0074] It delays the oxidation process of Omega-3 polyunsaturated fatty acids in the liquid oil-loaded nutrient, maintains its nutritional value, and can be used as a delivery system for nutrients to prolong the gastric emptying time in the gastrointestinal tract, thereby improving the digestion and absorption time and absorption utilization rate of nutrients in the body. Especially for dogs and cats with relatively short intestines, they can absorb nutrients in food more effectively and for a longer time.

[0075] The above-mentioned nano-liquid oil droplets in the embodiments of the present application are composed of vegetable oil substances, which are non-fish oil substances and pure plant raw materials, so there is no peculiar smell unique to fish oil.

[0076] As described above, for the formation of the three-dimensional network structure, first, by selecting an appropriate oil-phase gelling agent, such as candelilla wax, ethyl cellulose or other polymer gelling materials, and mixing it with an oil phase (including oil substances such as algal oil and linseed oil containing phospholipids). Then, yeast (such as brewer's yeast powder) and protein (such as whey protein) are added, and these components interact with each other under appropriate temperature and stirring conditions to form a stable network structure. In this process, the gelling agent forms a framework in the oil phase, while the protein and phospholipids help to enhance the stability and functionality of this structure.

[0077] The three-dimensional network structure can capture and encapsulate nano liquid oil droplets, aiming to control the size and distribution of the oil droplets, so as to achieve the sustained-release effect of active ingredients in the oil (such as Omega-3 polyunsaturated fatty acids). These nano-scale oil droplets are more easily absorbed by the pet's body, while reducing the contact of the oil droplets with the external environment (such as air, light, heat), reducing the oxidation risk, extending the shelf life of the product, and improving the nutritional value.

[0078] Phospholipids in plant-derived oil substances are crucial for the formation of the three-dimensional network structure, because phospholipids have amphiphilic properties (both hydrophilic and lipophilic), which enables them to form a stable emulsifying interface between the aqueous phase and the oil phase. This property of phospholipids helps to enhance the stability of the oil-based composition, improve the dispersibility of the oil droplets, and thus improve the physical and chemical stability of the whole composition. In addition, phospholipid-based Omega-3 polyunsaturated fatty acids (such as DHA and EPA) have higher bioavailability, which means they can be more effectively absorbed and utilized by the pet's digestive system, thus improving the nutritional effect of the product.

[0079] In summary, through a carefully designed formula and preparation process, the technical solution of this application utilizes the properties of phospholipids to form a three-dimensional network structure that can capture and encapsulate nano liquid oil droplets. This structure not only improves the stability and nutritional value of the product, but also enhances its sustained-release effect, providing a safer and more effective solution for pet hair beauty, hair care and health care.

[0080] In the embodiments of this application, the liquid plant-derived oil substances loaded with nutrients are coagulated by a gelling agent to reduce the contact between the load and air and delay the rate of the oxidation reaction. Then, the physical barrier provided by the three-dimensional network structure of the oil-based composition delays the release of the nutrients, so as to achieve the controlled release of these nutrients. Finally, protein encapsulation is achieved through emulsification homogenization and vacuum / freeze drying, and ultimately the function of sustained release of the liquid oil loaded with nutrients in the gastrointestinal tract can be realized.

[0081] In the embodiments of the present application, the end product of the nano-sustained-release oil-based composition can be a semi-fluid gel-like product, which can be mixed with common staple foods or snacks on the market for feeding without affecting the feeding preference for the original feed.

[0082] In addition, the nano-sustained-release oil-based composition has a certain viscosity and can achieve a sustained-release effect. The end product can effectively prolong the gastric emptying time, increase the duration of satiety after pets eat, and achieve the effect of reducing the overall food intake and frequency, so as to achieve the purpose of energy control and weight loss for animals (pets) with specific needs.

[0083] Furthermore, the plant-derived oil substances include algal oil and linseed oil.

[0084] In traditional animal hair beauty and care products, fish oil is widely popular due to its rich Omega-3 polyunsaturated fatty acids. However, due to its morphological characteristics, there are some inevitable shortcomings. Specifically, it is difficult to comprehensively optimize fish oil in terms of oxidation stability, digestion and absorption rate, retention of high-content components, reduction of heavy metal risks, and cost control. These limitations have affected the wide application of fish oil as an ideal nutritional supplement.

[0085] However, DHA and EPA in fish oil are not directly synthesized by deep-sea fish, but are derived from marine microorganisms in their food chain. In fact, marine microorganisms, especially marine microalgae, are the original producers of Omega-3 polyunsaturated fatty acids. As the oldest photosynthetic organisms on Earth, marine microalgae can grow rapidly using sunlight, water, and carbon dioxide. Therefore, the algal oil resources they provide are a sustainable and green and clean plant-based raw material in the production process. Compared with deep-sea fish oil, microalgal oil shows significant advantages in terms of safety, absorption and utilization rate, source stability, flavor, allergenicity, and heavy metal pollution risk, and can meet the needs of groups allergic to marine products for Omega-3 polyunsaturated fatty acids such as DHA and EPA.

[0086] Furthermore, the nano-sustained-release oil-based composition includes the following components in parts by weight:

[0087] 30 to 70 parts of algal oil. For example, the algal oil can be 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, etc. 20 to 60 parts of linseed oil; for example, the linseed oil can be 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, etc. 5 to 20 parts of protein; for example, the protein can be 5 parts, 8 parts, 10 parts, 15 parts, 20 parts, etc. 0.6 to 10 parts of gelling agent, for example, the gelling agent can be 0.6 parts, 0.8 parts, 1 part, 2 parts, 5 parts, 8 parts, 10 parts, etc. 0.1 to 3 parts of yeast; for example, the yeast can be 0.1 part, 0.3 part, 0.5 part, 0.8 part, 1 part, 2 parts, 3 parts, etc.

[0088] Furthermore, the protein is whey protein. Furthermore, the yeast is Saccharomyces cerevisiae.

[0089] As mentioned above, brewer's yeast powder is a safe, reliable and nutritious edible microorganism. It contains rich B vitamins, minerals, dietary fiber, β-glucan, glutathione, zymosan, more than 50% protein and amino acids and other nutrients. It is a functional food raw material of natural origin and an ideal thallus protein source for pets. It helps to improve the digestive ability, growth and development and immunity of animals (pets). Due to its rich unique flavor substances, it can not only improve the nutritional value of feed, but also improve the taste of feed in feed application.

[0090] Furthermore, the algal oil includes at least one of DHA algal oil, EPA algal oil and Haematococcus pluvialis oil.

[0091] As mentioned above, Haematococcus pluvialis is the best source of natural astaxanthin. Astaxanthin has strong antioxidant ability and also has functions such as anti-aging and prevention of cardiovascular and cerebrovascular diseases. Since when ω-3 polyunsaturated fatty acids are oxidized, they will emit bad odors and cause the product quality to decline. Therefore, scientifically compounding Haematococcus pluvialis oil containing natural astaxanthin with Schizochytrium sp. oil and Nannochloropsis sp. oil in a certain proportion can further avoid the oxidation, quality reduction and emission of bad odors of ω-3 polyunsaturated fatty acids during the production process and shelf life.

[0092] In the embodiments of the present application, the raw material composition and ratio are optimized, the use of non-nutritive additives is reduced, and the ratio of DHA and EPA is precisely adjusted to ensure the high-quality nutritional ratio of ω-3 polyunsaturated fatty acids such as DHA and EPA.

[0093] Furthermore, the algal source range of the DHA algal oil includes at least one of Schizochytrium sp., Ulkenia sp. and Crypthecodinium cohnii.

[0094] Furthermore, the algal source range of the EPA algal oil includes Nannochloropsis sp.

[0095] Preferably, the weight parts of the DHA algal oil in the algal oil are 15 parts to 40 parts; the weight parts of the EPA algal oil are 10 parts to 35 parts; the weight parts of the Haematococcus pluvialis oil are 0.2 parts to 3.5 parts.

[0096] As described above, the oil of Nannochloropsis is rich in polar lipids (phospholipids and glycolipids) and EPA in the form of phospholipids. The ω-3 unsaturated fatty acids in the form of phospholipids are less prone to oxidation, and EPA and DHA in the form of phospholipids have better bioavailability and biosafety than those in the form of ethyl esters and glycerides. In addition, cell membranes are mainly composed of elastic semi-permeable membranes made of phospholipids, so phospholipid-type EPA can be transported, absorbed and utilized in the body more efficiently.

[0097] As described above, phospholipids in polar lipids, as components of cell membranes and carriers of biological information, participate in many important physiological and pathological processes. And one of the important components in nerve membranes is DHA, which exists in 30%-40% of the phospholipids in the gray matter of the cerebral cortex and photoreceptor cells of the retina. Phospholipid DHA in phospholipid-type Omega-3 is the direct precursor of lysophosphatidylcholine DHA / EPA, and can cross the blood-brain barrier directly to the brain under the action of the specific transport protein MFSD2A, and its bioavailability can reach 1.8-2.4 times that of ethyl ester-type and triglyceride-type Omega-3. It can also effectively improve glucose metabolism disorders and lipid metabolism disorders, thus inhibiting the occurrence of seborrheic alopecia.

[0098] Phospholipid-type DHA / EPA can also produce an anti-inflammatory effect by reducing the release of inflammatory factors. Appropriate intake of phospholipid-type DHA and EPA can keep the hair and skin healthy, promote hair growth, improve hair coverage, maintain hair follicle health, and be beneficial to inflammation, cardiovascular, blood lipids and cognition. Therefore, when the oil of Nannochloropsis rich in EPA is combined with the oil of other algae rich in DHA (such as Schizochytrium, Ulkenia and Crypthecodinium cohnii) in a certain proportion, it can have great bioavailability.

[0099] Some microalgae species have attracted attention because they contain high concentrations of DHA. These algal oils are mainly cultivated through microbial fermentation technology to obtain varieties rich in DHA, and safer and higher-quality DHA is extracted from them. The production process of DHA algal oil is carried out in a clean and fully controllable automated fermentation system, ensuring the stability of its quality, the controllability of quality, the traceability of the whole process and low pollution. The whole production process reflects the characteristics of environmental protection. Therefore, DHA algal oil is not only a better source of Omega-3, but also a more environmentally friendly choice.

[0100] In the embodiments of the present application, the synergistic effect of each component between the raw materials is fully utilized. When the oil of Nannochloropsis rich in EPA is compounded with the oil of other algae rich in DHA (such as Schizochytrium, Ulkenia and Crypthecodinium cohnii), the phospholipids in the oil of Nannochloropsis can increase the bioavailability of DHA and EPA in the composition to 1.6-4.1 times that of ethyl ester-type and triglyceride-type Omega-3 polyunsaturated fatty acids, and a higher bioavailability can also be achieved compared with using phospholipid-type DHA or phospholipid-type EPA raw materials alone.

[0101] In addition, due to the different existing forms of algal oil with different species ranges and different sources, the Nannochloropsis oculata algal oil exists in the form of a high-viscosity solid paste at room temperature because of its relatively high polar lipid content and rich polar bonds. Therefore, according to the state differences of different algal oils, the direct method and the indirect method are used in combination when preparing the composite oil-based coagulant. A large number of polar bonds in the Nannochloropsis oculata algal oil also play a functional structural role in the composition of the three-dimensional network structure, making the overall system of the oil-based coagulant more stable. Then, through the nanoemulsification-vacuum / freeze-drying process of the oil-based coagulant, the composite oil-based coagulant system is nanoemulsified and protein-coated, and finally dried and dehydrated to obtain a stable nanoemulsified composite oil-based composition. Moreover, the product stability and palatability are significantly improved, the gastrointestinal adverse reactions are significantly reduced, the gastric emptying time is prolonged, and the overall antioxidant property is enhanced.

[0102] Further, the whey protein includes at least one of isolated whey protein, concentrated whey protein, hydrolyzed whey protein, and demineralized whey powder.

[0103] Further, the gelling agent includes an aqueous-phase gelling agent and an oil-phase gelling agent.

[0104] Among them, the aqueous-phase gelling agent includes at least one of gelatin, xanthan gum, sodium alginate, chitosan, galactomannan, pectin, and gellan gum.

[0105] The oil-phase gelling agent includes at least one of ethyl cellulose, hydroxypropyl methyl cellulose, and candelilla wax.

[0106] Among them, the weight parts of the aqueous-phase gelling agent are 0.1 part to 5 parts; the weight parts of the oil-phase gelling agent are 0.5 part to 8 parts. For example, the weight parts of the aqueous-phase gelling agent can be 0.1 part, 0.5 part, 0.8 part, 1 part, 3 parts, 5 parts, etc.; the weight parts of the oil-phase gelling agent can be 0.5 part, 0.8 part, 1 part, 3 parts, 5 parts, 8 parts, etc.

[0107] Reference Figure 2 , the embodiments of the present application provide a preparation method of a nano-sustained-release oil-based composition as described in any one of the foregoing embodiments, including:

[0108] Step S1, mixing the protein, the aqueous-phase gelling agent, and water under a first heating condition with a first stirring to obtain an aqueous-phase mixture.

[0109] As described above, a protein (such as whey protein), an aqueous gelling agent (such as gelatin), and water are subjected to a first stirring and mixing treatment under a first heating condition to obtain an aqueous mixture. Among them, heating and stirring help the protein and the aqueous gelling agent to be uniformly dispersed in water, forming a stable aqueous base, laying a foundation for subsequent emulsification and the preparation of an oil-based composition. A stirrer and a constant temperature water bath can be used to control the temperature and stirring speed.

[0110] The combination of the protein and the aqueous gelling agent provides a basis for the subsequent formation of a three-dimensional network structure.

[0111] As described above, the temperature of the first heating condition is not higher than 45 °C.

[0112] As described above, the rotation speed of the first stirring and mixing treatment is 100 r / min to 2000 r / min; for example, the rotation speed can be 100 r / min, 300 r / min, 500 r / min, 1000 r / min, 1500 r / min, 2000 r / min, and so on.

[0113] As described above, the time of the first stirring and mixing treatment is 5 minutes to 30 minutes; for example, the time can be 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, and so on.

[0114] Step S2, linseed oil and EPA algal oil in algal oil are subjected to a second stirring and mixing treatment under a second heating condition to obtain an oil-phase mixture.

[0115] As described above, linseed oil and algal oil (EPA algal oil) are subjected to a second stirring and mixing treatment under a second heating condition to obtain an oil-phase mixture. Heating helps the fluidity of the oil substances, and stirring ensures the uniform mixing of the oil substances, preparing for the formation of nano oil droplets.

[0116] An oil bath and a stirrer can be used to control the temperature and stirring rate. Among them, the mixing of different oil substances provides diverse nutritional components and functional characteristics.

[0117] As described above, the temperature of the second heating condition is 60 °C to 90 °C; for example, the temperature can be 60 °C, 70 °C, 80 °C, 90 °C, and so on.

[0118] As described above, the stirring time of the second stirring and mixing treatment is 15 minutes to 30 minutes; for example, the time can be 15 minutes, 20 minutes, 25 minutes, 30 minutes, and so on.

[0119] As described above, the stirring rate of the second stirring and mixing treatment is 60 r / min to 500 r / min; for example, the rotation speed can be 60 r / min, 100 r / min, 200 r / min, 300 r / min, 400 r / min, 500 r / min, and so on.

[0120] It should be noted that when performing the second stirring and mixing treatment, it can be preheated for 10 minutes to 30 minutes first, and then the stirring paddle is turned on for stirring. The purpose is to reduce the viscosity of the oil and improve its fluidity, making the oil substances easier to mix. Preheating helps the uniform dissolution and interaction of oil components, enhances the emulsification effect, ensures the temperature balance of the whole mixture, and avoids local overheating or overcooling, thereby improving the uniformity of stirring and mixing. In addition, preheating can also reduce the formation of bubbles during the stirring process, and these bubbles may affect the quality and stability of the final oil-based composition. Therefore, preheating is a key step in the preparation process, which lays a solid foundation for the formation of a uniform and stable oil-based composition.

[0121] Step S3: Add the oil-phase gelling agent and yeast to the oil-phase mixture, and perform a third stirring and mixing treatment under the second heating condition to obtain an oil-based condensate.

[0122] As described above, the oil-phase gelling agent and yeast are added to the oil-phase mixture, and a third stirring and mixing treatment is performed under the second heating condition to carry out an appropriate "Maillard reaction", thereby obtaining an oil-based condensate. The addition of yeast and the oil-phase gelling agent helps to form a three-dimensional network structure of the oil-based condensate and capture oil droplets.

[0123] As described above, the second heating condition is the heating condition in step S2, and an appropriate Maillard reaction is carried out while maintaining the oil bath heating state. The purpose is to enhance the flavor of the oil-based condensate and improve its palatability. The flavor compounds generated by this reaction can enhance the animal's preference for the product. At the same time, the Maillard reaction helps to activate the gelling agent and enhance the molecular interaction between proteins and other components, thereby forming a more stable three-dimensional network structure of the oil-based condensate. In addition, the mild oil bath heating environment helps to control the speed of the Maillard reaction and avoid the formation of bad flavors and colors caused by too fast a reaction. The Maillard reaction can also improve the digestibility and absorption rate of proteins, increase the bioavailability of nutrients in the product, and enhance the stability and shelf life of the product, which is crucial for improving the overall quality and performance of the oil-based composition product.

[0124] Specifically, an oil bath and a stirrer can be used to adjust the temperature and stirring rate to adapt to the newly added components. The combination of the oil-phase gelling agent and yeast enhances the structural stability of the oil-based condensate.

[0125] As described above, the stirring rate of the third stirring and mixing treatment is 300 r / min to 3000 r / min; for example, the rotation speed can be 300 r / min, 500 r / min, 800 r / min, 1000 r / min, 1500 r / min, 2000 r / min, 2500 r / min, 3000 r / min, and so on.

[0126] As described above, the stirring time of the third stirring and mixing treatment is 5 minutes to 30 minutes; for example, the time can be 5 minutes, 10 minutes, 15 minutes, 20 minutes, 25 minutes, 30 minutes, and so on.

[0127] Step S4: Cool the oil-based condensate, and add the DHA algal oil and Haematococcus pluvialis oil in the algal oil to the aqueous phase mixture respectively to obtain an oil-water condensate; and perform the third stirring and mixing treatment, emulsification treatment and high-pressure homogenization treatment on the oil-water condensate, so that the protein in the oil-water condensate, the phospholipids in the EPA algal oil, the aqueous phase gelling agent and the oil phase gelling agent form a three-dimensional network structure, and capture and encapsulate the dispersed nano liquid oil droplets to form a composite oil-based condensate-emulsion system.

[0128] As described above, cool the oil-based condensate, and add it to the aqueous phase mixture together with the DHA algal oil and Haematococcus pluvialis oil to obtain an oil-water condensate. Among them, cooling helps to control the reaction rate and the formation of the gel structure, and the addition of the DHA algal oil and Haematococcus pluvialis oil provides additional nutritional components for the product.

[0129] Specifically, it can be cooled by natural cooling or a cold water bath and then mixed.

[0130] It should be noted that in the algal oil used in the method provided in this embodiment, including EPA algal oil, DHA algal oil and Haematococcus pluvialis oil, in step S2, it is necessary to first add the EPA algal oil in the algal oil for heating and mixing, while other components in the algal oil such as DHA algal oil and Haematococcus pluvialis oil need to be added in subsequent step S4. The reason is determined by the nature of different algal oil materials itself. First of all, the EPA algal oil is in the form of a high-viscosity paste, and this kind of algal oil needs to be heated and softened first, and then diluted with linseed oil to become a liquid oil-phase mixture; while the DHA algal oil and Haematococcus pluvialis oil are easily oxidized at high temperatures, so the DHA algal oil and Haematococcus pluvialis oil are placed in step S3 and S4 for cooling and then fed for use during the preparation process, so as to avoid the decomposition or invalidation of components due to oxidation and ensure the effectiveness of the nutritional and functional components in the final product.

[0131] In this step, the mixing of the oil phase and the water phase lays the foundation for forming a stable emulsion system.

[0132] Further, step S4 includes:

[0133] Step S41: Cool the oil-based condensate to below 70°C.

[0134] Step S42: Take DHA algal oil and Haematococcus pluvialis oil, add them to the oil-based condensate after the cooling treatment, and perform the second stirring and mixing treatment to obtain an oil-based condensate-DHA algal oil mixture.

[0135] Step S43: Add the oil-based condensate-DHA algal oil mixture to the aqueous phase mixture to obtain the oil-water condensate.

[0136] Step S44: Perform a fourth stirring and mixing treatment, an emulsification treatment, and a high-pressure homogenization treatment on the oil-water condensate in sequence, so that the proteins in the oil-water condensate, the phospholipids in the EPA algal oil, and the aqueous phase gelling agent and the oil phase gelling agent form a three-dimensional network structure, and capture and encapsulate the dispersed nano liquid oil droplets to form the composite oil-based condensate-emulsion system.

[0137] Among them, the weight ratio of the added DHA algal oil and Haematococcus pluvialis oil to the oil-based condensate is: (10 - 45):(0.2 - 3.5):(30 - 80). It should be noted that the oil-based condensate here is the oil-based condensate that has been cooled to 70°C in step S41.

[0138] In the above ratio, the weight fraction of DHA algal oil can be 10, 20, 30, 40, 45, etc.; the weight fraction of Haematococcus pluvialis oil can be 0.2, 0.8, 1.5, 2.5, 3.5, etc.; the weight fraction of the oil-based condensate can be 50, 60, 70, 80, etc.

[0139] Among them, the ratio of the oil-based condensate-DHA algal oil mixture to the aqueous phase mixture is 1:(5 - 10). For example, it can be 1:5, 1:6, 1:7, 1:8, 1:10, etc.

[0140] As described above, a three-dimensional network structure is formed by performing a third stirring and mixing treatment, an emulsification treatment, and a high-pressure homogenization treatment on the oil-water condensate, and the nano liquid oil droplets are captured and encapsulated, thereby forming a composite oil-based condensate-emulsion system.

[0141] The formation of the three-dimensional network structure helps to stabilize the nano oil droplets and improve the sustained-release performance of the product.

[0142] Specifically, a high-speed stirrer can be used to achieve sufficient emulsification of the oil-water condensate.

[0143] The above-mentioned emulsification treatment method can be to add the oil-based condensate-DHA algal oil mixture to the aqueous phase mixture at 30°C to 60°C, and perform high-speed shear emulsification. The shear stirring speed is 3000 r / min to 10000 r / min, and the shear dispersion time is 5 min to 10 min.

[0144] The above-mentioned high-pressure homogenization treatment can be to degas the emulsified material and then add it to a high-pressure homogenizer. The homogenization pressure is 15 Mpa to 60 Mpa. Calculated per kilogram, the online circulation is 2 min to 10 min.

[0145] Among them, the synergistic effect of protein, phospholipid and gelling agent forms a stable emulsification system.

[0146] Through the treatment of this step, the molecules of the oil and water systems form protein-phospholipid aggregates through hydrogen bonds, hydrophobic interactions, van der Waals forces, phospholipid bonds, etc. Under the combined action of phospholipids and gelling agents in EPA algal oil, a three-dimensional network structure is formed to capture the homogenously dispersed liquid oil droplets into nanoscale ones, forming a composite oil-based condensate nanoemulsion (composite oil-based condensate-emulsification system).

[0147] Step S5: Perform a drying treatment on the composite oil-based condensate-emulsification system to obtain a nano-sustained release oil-based composition.

[0148] The above-mentioned drying treatment of the composite oil-based condensate-emulsification system to obtain a nano-sustained release oil-based composition, thereby obtaining the final product.

[0149] The above-mentioned drying treatment helps to control the moisture content of the product and improve the stability and shelf life of the product.

[0150] Specifically, vacuum drying (vacuum drying under reduced pressure) or freeze-drying technology can be used. The drying process helps to fix the structure of the emulsification system and ensure the long-term stability of the product.

[0151] The steps in this method mainly involve physical mixing, emulsification and drying processes. The synergistic effect between the steps is mainly reflected in the interaction of different components, forming a stable three-dimensional network structure and emulsification system, thereby improving the stability, sustained release performance and nutritional value of the product.

[0152] The oil-based composition provided in the embodiments of the present application is a semi-solid composition formed by a gelling agent, yeast, protein, and phospholipids in vegetable-derived oils constructing a three-dimensional network structure through self-assembly or interaction, capturing and encapsulating nano liquid oil droplets dispersed in the three-dimensional network structure. It has thermoreversibility (melting upon heating and solidifying upon cooling) and plasticity, combining the functions of liquid oil and solid stability. It delays the oxidation process of the liquid oil-loaded nutrient Omega-3 polyunsaturated fatty acids, maintains their nutritional value, and can be used as a delivery system for nutrients to prolong the gastric emptying time in the gastrointestinal tract, thereby enhancing the digestion and absorption time and absorption utilization rate of nutrients in the body. Especially for dogs and cats with relatively short intestines, they can absorb nutrients in food more effectively and for a longer time.

[0153] As described above, in step S5, the drying treatment includes heat vacuum drying or freeze vacuum drying. Among them, the temperature range of heat vacuum drying can be 20°C to 45°C.

[0154] It should be noted that the selection of the drying method depends on the product properties reflected by the actual composition of the formula. If the product has a low viscosity and high fluidity, freeze vacuum drying technology is preferably used; otherwise, heat vacuum drying technology is used.

[0155] As described above, in step S5, the water content of the nano-sustained-release oil-based composition is controlled not to exceed 0.5% through the drying treatment.

[0156] In step S1 of some embodiments, the weight parts of the protein, the aqueous gelling agent, and water are: protein 5 parts to 20 parts; for example, the protein can be 5 parts, 7 parts, 9 parts, 10 parts, 15 parts, 20 parts, etc. The aqueous gelling agent 0.1 part to 5 parts; for example, the aqueous gelling agent can be 0.1 part, 0.5 part, 0.8 part, 1 part, 3 part, 5 part, etc. Water 350 parts to 850 parts. For example, water can be 350 parts, 400 parts, 500 parts, 600 parts, 700 parts, 850 parts, etc. Among them, water can include, but is not limited to, deionized water, distilled water, ultrapure water, etc.

[0157] In step S2 of some embodiments, the weight parts of linseed oil and EPA algal oil are: linseed oil 20 parts to 60 parts; for example, linseed oil can be 20 parts, 30 parts, 40 parts, 50 parts, 60 parts, etc. EPA algal oil 10 parts to 35 parts; for example, EPA algal oil can be 10 parts, 20 parts, 30 parts, 35 parts, etc.

[0158] In step S3 of some embodiments, the weight ratio of the oil-phase gelling agent and yeast added to the oil-phase mixture is (0.5-8):(0.1-3):(30-80). For example, the oil-phase gelling agent in the ratio can be 0.5 part, 1 part, 2 parts, 4 parts, 8 parts, etc.; the yeast can be 0.1 part, 0.6 part, 1 part, 2 parts, 3 parts, etc.; the oil-phase mixture can be 30 parts, 40 parts, 50 parts, 60 parts, 70 parts, 80 parts, etc.

[0159] In the embodiments of the present application, there is provided a nano-sustained-release oil-based composition as described in the foregoing embodiments, or a feeding method of the nano-sustained-release oil-based composition prepared by the preparation method of the nano-sustained-release oil-based composition as described in the foregoing embodiments. The feeding method includes at least one of the following methods: A. Directly feeding the nano-sustained-release oil-based composition. B. Mixing the nano-sustained-release oil-based composition with animal feed and then feeding. C. Covering the surface of the animal feed with the nano-sustained-release oil-based composition and then feeding. Among them, the feed includes food and / or medicine; the food includes at least one of granular dry food, wet food, and freeze-dried meat granules.

[0160] The nano-sustained-release oil-based composition provided in the embodiments of the present application can effectively achieve the purpose of beautifying and protecting the hair. And compared with conventional products, it is more popular with animals (such as pets) in terms of flavor, avoiding food waste caused by refusal to eat during the feeding process. In terms of product ingredients, it is safer and more concise, and the absorption and utilization rate has been greatly improved, with less digestive pressure on the gastrointestinal system. Through the nano-sustained-release process, EPA algal oil and DHA algal oil are dispersed and encapsulated, effectively reducing adverse reactions such as flatulence, diarrhea, and nausea after ingestion, avoiding the risk of short-term anticoagulation dysfunction caused by rapid absorption of EPA and the risk of heavy metal accumulation under long-term use, reducing the overall digestive system burden of pets, and improving the duration of satiety of pets when mixing with food.

[0161] Furthermore, a small amount of brewer's yeast powder is added as a supplement in terms of flavor, increasing the umami taste of this formula, reducing the rejection psychology of pets towards the new dosage form, enhancing the desire to try, and helping to improve the palatability and digestive function of pets.

[0162] In addition, such a nano-sustained-release oil-based composition can also be used as one of the premixes of conventional products and added to different products according to a certain ratio, so as to achieve the effect of enhancing the hair-beautifying effect of the marketed products.

[0163] In an embodiment of the present application, there is provided a nano-sustained release oil-based composition as described in the foregoing embodiment, or the nano-sustained release oil-based composition prepared by the preparation method of the nano-sustained release oil-based composition as described in the foregoing embodiment, for use in preparing an animal health product, where the animal health product includes at least one of a hair beautifying product and a weight loss product.

[0164] The above products include a hair beautifying product and a weight loss product. Their dosage forms can be dosage forms adapted according to the needs of the products. For example, it can be a product with a nano-sustained release oil-based composition as the coating and a specific component as the core, or a composite structure with a nano-sustained release oil-based composition as the core and a specific component as the coating. For another example, it can be in the form of separately packaged "AB" agents. For example, A is a nano-sustained release oil-based composition and B is the target substance. When used, they are unpacked separately and then covered, mixed, stirred, and encapsulated, and even other substances such as water, dairy product liquids, and wet food are added for joint feeding.

[0165] The present invention will be further illustrated by specific examples below. However, it should be understood that these examples are only for more detailed illustration and should not be construed as limiting the present invention in any form.

[0166] Table 1. Components and related parameters in the examples

[0167] Component / Parameter Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 DHA algal oil dosage 21.1 parts 39.5 parts 15 parts 51.1 parts 15 parts EPA algal oil dosage 30 parts 10 parts 16 parts × 16 parts Haematococcus pluvialis oil dosage 1.3 parts 3.4 parts 0.2 parts 1.3 parts 0.2 parts Flaxseed oil dosage 31 parts 20 parts 54.2 parts 31 parts 54.2 parts Protein type Whey protein Isolate whey protein Concentrate whey protein Whey protein × Protein dosage 10.6 parts 19.5 parts 5.17 parts 10.6 parts × Aqueous phase gelling agent Chitosan Sodium alginate Xanthan gum Chitosan × Aqueous phase gelling agent dosage 4.4 parts 1.0 part 2.46 parts 4.4 parts × Oil phase gelling agent type Ethyl cellulose Ethyl cellulose Candelilla wax Ethyl cellulose × Oil phase gelling agent dosage 0.5 part 7.3 parts 4.5 parts 0.5 part × Yeast dosage 1.1 parts 0.2 part 2.47 parts 1.1 parts × Water dosage 833 parts 380 parts 626 parts 833 parts × Mixing ratio 1:10 1:5 1:7 1:10 × Drying method Freeze-drying under vacuum Freeze-drying under vacuum Vacuum drying at 40°C Freeze-drying under vacuum ×

[0168] In Table 1, the "mixing ratio" represents the mixing ratio of the oil-based condensate - DHA algal oil mixture to the aqueous phase mixture; the "water" used is purified water; the "dosage" represents the added amount of the feedstock.

[0169] Example 1

[0170] In this example, there is provided a supplementary food oil agent (nano-sustained release oil-based composition) for general use in dogs and cats for hair beautifying and care, prepared according to the components in Table 1.

[0171] Preparation method:

[0172] (1) Preparation of the aqueous phase mixture: Weigh 10.6 parts of whey protein, 4.4 parts of chitosan, and 833 parts of purified water by weight. Pour them into mixing pot 1 and stir at a speed of 100 - 2000 r / min for 5 - 30 min under a water bath condition of 45°C to obtain the aqueous phase mixture.

[0173] (2) Preparation of oil-phase mixture: Weigh 30 parts of EPA algal oil (using Nannochloropsis oculata oil) and 31 parts of flaxseed oil by weight, pour them into mixing pot 2, heat them in an oil bath to 60 - 90 °C, preheat for 10 - 30 minutes, then turn on the stirring paddle. The stirring rate is 60 - 500 r / min, and the stirring time is 15 to 30 minutes to fully soften and dissolve the EPA algal oil blocks. After the above raw materials are fully mixed evenly, it is recorded as the oil-phase mixture.

[0174] (3) Preparation of oil-based condensate: Weigh 0.5 part of ethyl cellulose and 1.1 parts of brewer's yeast powder by weight and add them to the oil-phase mixture while it is hot. Keep the oil bath heating state and carry out an appropriate Maillard reaction. The stirring rate is 300 - 3000 r / min, and the stirring time is 5 - 30 minutes, which is recorded as the oil-based condensate.

[0175] (4) Preparation of oil-based condensate - DHA algal oil mixture: Wait until the temperature of the oil-based condensate material drops to 70 °C and below. Weigh 21.1 parts of Schizochytrium sp. oil and 1.3 parts of Haematococcus pluvialis oil by weight, and pour them into the oil-based condensate one by one. After fully stirring and mixing, the oil-based condensate - DHA algal oil mixture is obtained.

[0176] (5) Preparation of composite oil-based condensate - emulsion system: While it is hot, add the oil-based condensate - DHA algal oil mixture to the water-phase mixture at a ratio of 1:10 to obtain an oil-water condensate, and then through high-speed stirring, emulsification, and high-pressure homogenization treatment, a composite oil-based condensate - emulsion system is formed.

[0177] (6) Drying: Pass the composite oil-based condensate - emulsion system through a freeze-vacuum drying process to control the water content below 0.5% to obtain the product described in this formula, namely the nano-sustained release oil-based composition.

[0178] Example 2

[0179] In this example, a supplementary food oil agent (nano-sustained release oil-based composition) for dogs and cats that is common for beautifying and protecting hair is provided, and each 100 parts is prepared from the following components by weight.

[0180] Preparation method:

[0181] The method adopted in this example is basically the same as that in Example 1, and the differences refer to the components, dosages, and related parameters in Table 1.

[0182] Example 3

[0183] In this example, a supplementary food oil agent (nano-sustained release oil-based composition) for dogs and cats that is common for beautifying and protecting hair is provided, and each 100 parts is prepared from the following components by weight.

[0184] Preparation method:

[0185] The method adopted in this embodiment is basically the same as that in Embodiment 1, and the differences refer to the components, dosages and related parameters in Table 1.

[0186] Comparative Example 1

[0187] In this comparative example, a general supplementary food oil agent (nano-sustained release oil-based composition) for dogs and cats for beautifying and protecting hair is provided, which is prepared from the following components in parts by weight per 100 parts.

[0188] Preparation method:

[0189] The method adopted in this comparative example is basically the same as that in Embodiment 1, and the differences refer to the components, dosages and related parameters in Table 1.

[0190] Comparative Example 2

[0191] In this comparative example, a general supplementary food oil agent (mixed oil composition) for dogs and cats for beautifying and protecting hair is provided, which is prepared from the following components in parts by weight per 100 parts.

[0192] Preparation method:

[0193] The method adopted in this comparative example is the same as steps (2) and (4) of Embodiment 1, and the differences refer to the components, dosages and related parameters in Table 1.

[0194] Comparative Experiment Example 1: Investigation on the functions of beautifying hair and controlling body weight

[0195] 1. Experimental design grouping: Randomly select 20 golden retrievers over six months old (10 males and 10 females) and 20 Linqing lion cats over one year old (10 males and 10 females). Take 4 dogs (2 males and 2 females) and 4 cats (2 males and 2 females) as 1 group, and a total of 5 groups are set up. The 5 groups are respectively denoted as Group A (Embodiment 1 - low dose group), Group B (Embodiment 1 - medium dose group), Group C (Embodiment 1 - high dose group), Group D (commercially available fish oil group), and Group E (blank control group). Number the 4 dogs in each group from 1 to 4, and number the 4 cats from 5 to 8.

[0196] 2. Feeding scheme: Each group of dogs / cats is given sufficient basic dog food / basic cat food every day, with unlimited water and free access to food. Select Embodiment 1 and randomly select a commercially available hair-beautifying fish oil in a certain city as the additional nutritional supplement for Experimental Groups A - D, among which Groups A - D are given additional direct oral feeding. The feeding schemes of Embodiment 1 and the commercially available fish oil are shown in Table 2. Group E is used as the blank control group and is not given additional feeding. Feed continuously for 60 days, and each test animal is housed individually in a pet cage (1.8m × 0.8m × 0.8m). Disinfect and clean the feeding environment of dogs and cats before the experiment starts, and clean the pen every day to keep it clean. Observe the physiological and mental states of all test animals during the experimental period, and check whether there are adverse reactions such as aversion, diarrhea, vomiting, etc.

[0197] Table 2. Feeding Scheme

[0198]

[0199] In Table 2, the unit of each dose is g / kg wt; for groups A, B, C, and D, the feeding method is to suck the corresponding amount of the sample with a syringe and slowly inject it from the side of the mouth of the test animal, avoiding coughing and choking; group E is the blank control group and no additional feeding is carried out.

[0200] 3. Experimental Method:

[0201] (1) Determination of the component indicators of the test sample: Detect some key indicators.

[0202] Nutritional indicators: DHA+EPA, Omega-3 polyunsaturated fatty acids;

[0203] Physical and chemical indicators: peroxide value, acid value;

[0204] Pollutant indicators: arsenic, chromium, lead, cadmium, mercury;

[0205] Pesticide residue indicators: polychlorinated biphenyls, DDTs

[0206] (2) Sensory evaluation of the coat: A sensory evaluation panel consisting of 5 testers. The members of the sensory evaluation panel are in good health and have no ophthalmic diseases such as color blindness and color weakness. Conduct a double-blind sensory evaluation of the animal's coat, and the scoring criteria refer to Table 3, with a score range of 1-5 points.

[0207] Table 3. Sensory Scoring Criteria for the Coat

[0208]

[0209] (3) Determination of the fur indicators: On the 60th day, measure the fur indicators (skin moisture, skin oil content, skin pH, coat color difference value, coat elastic elongation rate and recovery rate, average coat length, coat fineness) of the test animals.

[0210] (4) Body weight indicators: Record the feed intake and weight gain of the test animals on the day of the start of the experiment, the 30th day, and the 60th day.

[0211] 4. Experimental Results

[0212] (1) Component indicators of the test sample: As can be seen from Table 4, there is no obvious difference in the nutritional component content between Example 1 and the commercially available hair beauty fish oil. In terms of pollution indicators and antioxidant conditions, Example 1 is superior.

[0213] Table 4. Component Indicators of Example 1 and a Certain Commercially Available Fish Oil

[0214] Composition Example 1 Commercially available hair beautifying fish oil DHA + EPA, g / 100g 25 29 Omega-3 polyunsaturated fatty acids, g / 100g 45 40 Peroxide value, g / 100g 0.042 0.090 Acid value, mg / g 0.18 0.46 Arsenic, mg / kg Not detected 0.083 Chromium, mg / kg Not detected 0.49 Lead, mg / kg Not detected Not detected Cadmium, mg / kg Not detected 0.02 Mercury, mg / kg Not detected 0.13 Polychlorinated biphenyls, μg / kg Not detected 4.039 DDTs (dichlorodiphenyltrichloroethane), μg / kg Not detected 28

[0215] (2) Sensory evaluation of the coat: As can be seen from Table 5, compared with the blank control group, there were significant differences (P < 0.05 or 0.01) in coat brightness, coat softness, and skin elasticity in the low, medium, and high dose groups of Example 1 and the commercially available fish oil group.

[0216] Table 5. Effects of Example 1 and a commercially available fish oil on the sensory indices of the coats of experimental dogs and cats

[0217]

[0218] Note: * indicates P value < 0.05; ** indicates P value < 0.01.

[0219] (3) Fur indices:

[0220] Table 6. Effects of Example 1 and a commercially available fish oil on the fur indices of experimental dogs and cats

[0221]

[0222] Note 1: In Table 6, * indicates P value < 0.05 and ** indicates P value < 0.01; Note 2: L * represents color brightness, a * represents the red - green value, b * represents the yellow - blue value.

[0223] 1) As can be seen from Table 6, compared with the blank control group, the low-dose group of Example 1 showed significant differences (P < 0.05 or 0.01) in the skin moisture of dogs, the hair color difference values (brightness, red-green values) of dogs, the hair color difference value (blue-yellow value) of cats, and the hair recovery rate. 2) The medium-dose group of Example 1 showed significant differences (P < 0.05 or 0.01) in the skin moisture, skin oil content, skin pH, hair color difference values (brightness, red-green values, blue-yellow values) of dogs, the hair recovery rate, the skin moisture, skin pH, hair color difference value (brightness), hair elastic elongation rate, and hair recovery rate of cats. 3) The high-dose group of Example 1 showed significant differences (P < 0.05 or 0.01) in the skin moisture, skin oil content, skin pH, hair color difference values (brightness, red-green values, blue-yellow values), hair elastic elongation rate, and hair recovery rate of dogs, and the skin moisture, skin oil content, hair color difference values (brightness, blue-yellow values), hair elastic elongation rate, and hair recovery rate of cats. 4) The commercially available fish oil group showed significant differences (P < 0.05 or 0.01) in the skin moisture, hair color difference values (brightness, red-green values, blue-yellow values) of dogs, the hair recovery rate, the hair color difference value (blue-yellow value) of cats, and the hair recovery rate. It can be seen that when Example 1 is ingested at a low dose, its hair beautifying effect on the hair is similar to that of the commercially available fish oil group; when ingested at a medium dose, its intake is similar to that of the commercially available fish oil group, but its hair beautifying effect on the hair is better than that of a certain commercially available fish oil; when ingested at a high dose, its intake is higher than that of a certain commercially available fish oil, and its hair beautifying effect on the hair is better than that of a certain commercially available fish oil and the medium-dose group of Example 1. From this, it can be inferred that the hair beautifying effect of Example 1 on the hair of dogs and cats is better than that of a certain commercially available fish oil.

[0224] (5) Body weight index: As can be seen from Tables 7 to 8, compared with the blank control group:

[0225] 1) In the low-dose group of Example 1, there were significant differences in the decrease in food intake of cats on the 30th and 60th days and the relative decrease in body weight gain on the 30th day (P < 0.05 or 0.01). 2) In the medium-dose group of Example 1, there were significant differences in the decrease in food intake of dogs on the 60th day and the relative decrease in body weight gain on the 30th day (P < 0.05 or 0.01), and there were significant differences in the food intake of cats on the 30th and 60th days and the relative decrease in body weight gain on the 60th day (P < 0.05 or 0.01). 3) In the high-dose group of Example 1, there were significant differences in the food intake and relative decrease in body weight gain of dogs and cats on the 30th and 60th days (P < 0.05 or 0.01). 4) In the commercially available fish oil group, there were significant differences in the decrease in food intake of cats on the 30th and 60th days and the relative decrease in body weight gain on the 60th day (P < 0.05 or 0.01). It can be seen that in Example 1, when the intake is at a low dose, its food intake and weight gain are similar to those of a commercially available fish oil group; when the intake is at medium and high doses, its food intake and weight gain are lower than those of a commercially available fish oil. From this, it can be inferred that Example 1 has a better effect on controlling the food intake and weight gain of dogs and cats than a commercially available fish oil.

[0226] Table 7. Food Intake and Weight Gain of Experimental Dogs and Cats in Example 1 and Commercially Available Fish Oil (ABC)

[0227]

[0228] Table 8. Food Intake and Weight Gain of Experimental Dogs and Cats in Example 1 and Commercially Available Fish Oil (DE)

[0229]

[0230] Note: * indicates P value < 0.05; ** indicates P value < 0.01.

[0231] 5. Conclusion: This experimental study investigated the effects of algal oil-based compositions and fish oil on the fur of pet dogs and cats. Through a 60-day feeding experiment on pet dogs and cats, the following conclusions can be drawn: (1) At the same dose, the algal oil-based composition makes the fur of pet dogs and cats look glossier visually, followed by fish oil. There is no obvious change in the fur appearance of the dogs and cats in the control group before and after feeding. (2) In different dose groups of both fish oil and algal oil-based compositions, improvements in the fur indices of pet dogs and cats can be observed. A low dose of the algal oil-based composition can achieve a fur beautifying effect similar to that of the fish oil group, and medium and high doses of the algal oil-based composition can achieve a better fur beautifying effect than commercially available fish oil. (3) Compared with the blank control group, the food intake and weight gain of dogs and cats in the medium and high dose groups are significantly reduced (p < 0.01). The food intake and weight gain of cats in the low dose group are significantly reduced (p < 0.05). The food intake and weight gain of dogs in the fish oil group and dogs in the low dose group are not significantly reduced (p > 0.05). (4) Based on the conclusions in (1) - (3), it can be concluded that Example 1 has a fur beautifying effect on the coats of pet dogs and cats, and at appropriate doses, it helps control food intake and slow down the trend of weight gain.

[0232] Comparative Experimental Example 2: Palatability Investigation

[0233] 1. Experimental Design: Randomly select 30 cats of any breed over 1 year old (15 males and 15 females). The experimental period is 4 days in total. During this period, the diet is added once a day at a fixed time and place. The test dogs and cats can move freely and drink water freely, and eat freely at any time. Before the experiment, the breeding environment of the dogs and cats is disinfected and cleaned, and the excreta of the dogs and cats are cleaned daily. 2. Experimental Method: Add 99 g of basic cat food and 1 g of the product prepared in Example 1 to Bowl A, and add 99 g of basic cat food and 1 g of commercially available fur beautifying fish oil (same as Comparative Experimental Example 1) to Bowl B. On the first day, place the two bowls in the cat cage at the same time, record the number of the bowl that the test cat eats from for the first time, and record whether it eats and the amount of food eaten after 24 hours. Swap the positions of the two bowls the next day and repeat the above experiment for 4 consecutive days. During the experiment, record the food intake and first-choice preference of the test dogs and cats every day. 3. Experimental Results: The data recorded in the double-bowl experiment are shown in Table 9 (a, b).

[0234] Table 9a. Daily first-choice preference and food intake ratio of test cats (1 - 15)

[0235]

[0236] Table 9b. Daily first-choice preference and food intake ratio of test cats (16 - 30, total)

[0237]

[0238] In Table 9, the ratio represents: single-bowl food intake / total daily food intake. It can be seen from Table 9 that both the preferred rate and the consumption ratio of Bowl A are higher than those of Bowl B added with commercially available hair-conditioning fish oil. Therefore, based on the experimental situation, it can be concluded that the test cats are more inclined to eat Bowl A added with Example 1. It can be confirmed that compared with commercially available hair-conditioning fish oil, cats are more inclined to choose the food added with the present invention when eating.

[0239] Comparative Experimental Example 3: Influence of phospholipids on the properties of the nano-sustained-release oil-based composition system and investigation of the corresponding hair-conditioning effect

[0240] 1. Experimental design and grouping: Randomly select 20 golden retrievers over six months old (10 males and 10 females). Divide 10 dogs (5 males and 5 females) into one group, for a total of 2 groups. The two groups are respectively denoted as Group A (using the product of Example 1) and Group B (using the product of Comparative Example 1). Number the 10 dogs in each group from 1 to 10.

[0241] 2. Feeding scheme: Each group of dogs is given a sufficient amount of basic dog food every day, with unlimited water and free access to food.

[0242] Group A and Group B are given additional direct oral feeding. The feeding scheme is shown in Table 10. Group C is used as a blank control group without additional feeding.

[0243] Continue feeding for 30 days. Each test animal is housed individually in a pet cage (1.8m × 0.8m × 0.8m).

[0244] Before the start of the experiment, disinfect and clean the feeding environment of dogs and cats. Clean the enclosure hygiene every day to keep it clean. Observe the physiological and mental states of all test animals during the experimental period to check for any adverse reactions such as aversion, diarrhea, vomiting, etc.

[0245] Table 10. Feeding scheme

[0246] NO. Grouping Dog (g / kg wt) 1 A (Example 1) 1.60 2 B (Comparative Example 1) 1.60 3 C (Blank control) /

[0247] In Table 10, the feeding methods for both Group A and Group B are to suck the corresponding amount of the sample with a syringe and slowly inject it from the side of the test animal's mouth, avoiding coughing and choking.

[0248] (1) Investigation of shaping effect: Use viscosity index and sensory evaluation to evaluate the shaping effect of Example 1 and Comparative Example 1. Verify whether the addition of phospholipid-rich Nannochloropsis oculata oil has a significant impact on the properties of the present invention.

[0249] (2) Sensory evaluation of the coat: A sensory evaluation panel consisting of 5 testers. The members of the sensory evaluation panel are in good health and have no ophthalmological diseases such as color blindness or color weakness. Conduct a double-blind sensory evaluation of the animal's coat. The scoring criteria refer to Table 11, with a score range of 1 - 5 points.

[0250] Table 11, Sensory Scoring Criteria for Coat

[0251]

[0252] (3) Determination of fur indicators: On the 30th day, the fur indicators (skin moisture, skin oil content, skin pH, coat color difference value, coat elastic elongation rate and recovery rate, average coat length, coat fineness) of the test animals were measured.

[0253] 4. Experimental Results

[0254] (1) Molding effect:

[0255] Table 12, Molding Effects of Example 1 and Comparative Example 1

[0256] Item Example 1 Comparative Example 4 Viscosity (mPa.s) 3208 1609

[0257] (2) Through the viscosity measurement and sensory evaluation of the products prepared in Example 1 and Comparative Example 1, it can be seen that the product in Example 1 is a semi-solid colloid with a certain viscosity, good formability, and poor fluidity. Its shape remains unchanged after standing for 24 hours; while the product in Comparative Example 1 is a colloid with good fluidity, poor formability, and a layering phenomenon of floating oil on the upper layer appears after standing for 8 hours. It can be seen that the phospholipid-rich Nannochloropsis oculata oil in the product obtained in Example 1 has a good improvement effect on the entire nano-sustained-release oil-based composition system.

[0258] (3) Sensory evaluation of coat:

[0259] Table 13, Influence of Example 1 and Comparative Example 1 on the Coat Sensory Indexes of Experimental Dogs

[0260] Group Hair color brightness Hair softness Skin elasticity A 4.3±0.82* 4.3±0.95** 4.2±0.79 B 3.4±0.84 3.1±0.88 3.9±0.32

[0261] Note: * indicates P value < 0.05; ** indicates P value < 0.01.

[0262] According to the results in Table 13, compared with the Comparative Example 1 group, there are significant differences (P < 0.05 or 0.01) in the coat brightness, coat softness, and skin elasticity of the Example 1 group.

[0263] (4) Fur indicators:

[0264] Table 14, Influence of Example 1 and Comparative Example 1 on the Coat Indexes of Experimental Dogs

[0265]

[0266] Note 1: * indicates P value < 0.05, ** indicates P value < 0.01; Note 2: L * represents color brightness, a *Represents the red-green value, b * Represents the yellow-blue value.

[0267] As can be seen from Table 14, compared with the control group 1, there were significant differences in skin moisture, skin oil content, coat color difference value, coat elastic elongation rate, coat recovery rate, coat average length, and coat fineness of the dogs in the experimental group 1 (P < 0.05 or 0.01).

[0268] From this, it can be inferred that the hair beautifying effect of the experimental group 1 containing phospholipids on the dog's coat is better than that of the control group 1 without phospholipids.

[0269] Comparative experimental example 4: Investigate the effects of the nano-sustained release oil-based composition on the food intake, feeding frequency, and weight control of cats

[0270] 1. Experimental design: Randomly select 20 cats of any breed over 1 year old (10 males and 10 females). Divide 10 cats (5 males and 5 females) into 1 group, for a total of 2 groups. The experimental period is 30 days. During this period, the diet is added once a day at a fixed time and place. The test cats can move freely, drink water freely, and eat freely at any time. Before the experiment, the breeding environment is disinfected and cleaned, and the excreta of the test cats are cleaned daily.

[0271] 2. Experimental method: The test cats in group A are fed 97 g of basic cat food and 3 g of the experimental example 3, and the test dogs in group B are fed 97 g of basic cat food and 3 g of the control group 2. Record the food intake and feeding times every day. Weigh each cat before the experiment and on the 30th day and record.

[0272] 3. The experimental results are shown in Table 15:

[0273] Table 15. Effects of experimental example 3 and control group 2 on the coat indexes of experimental cats

[0274] Group Group A Group B Daily feed intake (g) 62.57±11.41** 82.51±14.78 Daily feeding frequency (times) 2.2±0.42** 3.5±0.85 Weight change before and after the test (kg) 0.03±0.01* 0.05±0.02

[0275] Note: * indicates P value < 0.05, ** indicates P value < 0.01.

[0276] Compared with the control group 2, the daily food intake, daily feeding times, and weight changes before and after the experiment of the test cats in the experimental example 3 showed a downward trend, with significant differences (P < 0.05 or 0.01).

[0277] 4. Conclusion: Through this experiment, it can be seen that the food intake, feeding times, and weight changes before and after the experiment of the test cats in group A (experimental example 3) are lower than those in group B (control group 2), with significant differences, and the P value < 0.05. It can be seen that the nano-sustained release oil-based composition has a certain effect on satiety and weight control compared with the mixed oil composition.

[0278] Comparative Experiment Example 5: Comparison of in vitro digestion times of nano-sustained release oil-based compositions in a simulated gastric environment

[0279] 1. Experimental design: Add 5 mL of the test sample to a test tube containing hydrochloric acid (pH = 1.5), seal it, and place it in a thermostatic water bath oscillator with the temperature set at 39°C and the oscillation speed at 100 rpm to simulate the digestion progress in the gastric digestion environment of dogs and cats.

[0280] 2. Experimental method: Use the products prepared in Example 1, Example 2, Example 3, and Comparative Example 2 as test samples, and label them as groups A, B, C, and D respectively. Take 5 mL of each example and add it to a test tube containing hydrochloric acid (pH = 1.5), seal it, and place it in a thermostatic water bath oscillator with the temperature set at 39°C and the oscillation speed at 80 rpm. Set 5 parallel samples in each group (numbered 1 - 5). Record the time it takes for the test sample to be completely digested to no residue in the test tube.

[0281] 3. The experimental results are shown in Table 16:

[0282] Table 16. In vitro digestion times (minutes) of Examples 1 - 3 and Comparative Example 2

[0283]

[0284] Note: * indicates P value < 0.05, ** indicates P value < 0.01.

[0285] 4. Conclusion:

[0286] Compared with the mixed oil composition, the digestion time of the nano-sustained release oil-based composition in the simulated gastric digestion environment is prolonged, showing a significant difference (P < 0.05 or 0.01). It is thus speculated that the compositions provided in Examples 1 - 3 can effectively prolong the gastric emptying time in vivo and extend the duration of satiety.

[0287] In addition, no gastrointestinal problems occurred in the pets during the above experimental process, indicating that the dietary supplement involved in the present invention does not cause adverse reactions to the digestive systems of dogs and cats.

[0288] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A nano slow-release oil-based composition, characterized in that: The nano slow-release oil-based composition is a composition consisting of a three-dimensional network structure formed by a gelling agent, yeast, protein and phospholipids in plant-derived oil substances, which captures and encloses nano liquid oil droplets dispersed in the three-dimensional network structure; wherein the nano liquid oil droplets are nano liquid oil droplets formed by plant-derived oil substances.

2. The nano sustained-release oil-based composition according to claim 1, characterized in that: The plant-derived oil substances include algae oil and linseed oil; Preferably, the nano sustained-release oil-based composition comprises the following components in parts by weight: 30 to 70 parts of algae oil; 20-60 parts of linseed oil; 5 to 20 servings of protein; 0.6 to 10 parts of gelling agent; 0.1 to 3 parts of yeast; Preferably, the protein is whey protein; Preferably, the yeast is brewer's yeast.

3. The nano slow-release oil-based composition according to claim 2, characterized in that: The algae oil includes at least one of DHA algae oil, EPA algae oil and Haematococcus pluvialis oil; Preferably, the algae source range of the DHA algae oil includes at least one of Schizochytrium, Ukenichthys and Crypthecodinium cohnii; Preferably, the algae source range of the EPA algae oil includes Nannochloropsis spp.; Preferably, the weight proportion of DHA algae oil in the algae oil is 15 to 40 parts; The weight proportion of the EPA algae oil is 10 to 35 parts; The weight proportion of the Haematococcus pluvialis oil is 0.2 to 3.5 parts.

4. The nano slow-release oil-based composition according to claim 2, characterized in that: The whey protein comprises at least one of isolated whey protein, concentrated whey protein, hydrolyzed whey protein and desalted whey powder.

5. The nano slow-release oil-based composition according to claim 2, characterized in that: The gelling agent includes a water phase gelling agent and an oil phase gelling agent; Preferably, the aqueous phase gelling agent includes at least one of gelatin, xanthan gum, sodium alginate, chitosan, galactomannan, pectin, and gellan gum; Preferably, the oil phase gelling agent comprises at least one of ethyl cellulose, hydroxypropyl methyl cellulose and candelilla wax; Preferably, the weight proportion of the water phase gelling agent is 0.1 to 5 parts; the weight proportion of the oil phase gelling agent is 0.5 to 8 parts.

6. A method for preparing the nano sustained-release oil-based composition according to any one of claims 1 to 5, characterized in that: include: S1, subjecting the protein, the aqueous phase gelling agent and water to a first stirring and mixing treatment under a first heating condition to obtain an aqueous phase mixture; S2, subjecting the linseed oil and the EPA algae oil in the algae oil to a second stirring and mixing process under a second heating condition to obtain an oil phase mixture; S3, adding the oil phase gelling agent and yeast to the oil phase mixture, and performing a third stirring and mixing process under the second heating condition to obtain an oil-based coagulant; S4, cooling the oil-based coagulant, and adding the DHA algae oil and the Haematococcus pluvialis oil in the algae oil to the aqueous phase mixture, respectively, to obtain an oil-water coagulant; and performing the third stirring and mixing treatment, emulsification treatment and high-pressure homogenization treatment on the oil-water coagulant, so that the protein in the oil-water coagulant, the phospholipids in the EPA algae oil, the aqueous phase gelling agent and the oil phase gelling agent form a three-dimensional network structure, and capture and encapsulate the dispersed nano-liquid oil droplets to form a composite oil-based coagulant-emulsification system; S5, drying the composite oil-based coagulant-emulsified system to obtain a nano slow-release oil-based composition; Preferably, the temperature of the first heating condition is not higher than 45°C; Preferably, the rotation speed of the first stirring and mixing process is 100 r / min to 2000 r / min; Preferably, the first stirring and mixing treatment lasts for 5 minutes to 30 minutes; Preferably, the temperature of the second heating condition is 60°C to 90°C; Preferably, the stirring time of the second stirring and mixing treatment is 15 minutes to 30 minutes; Preferably, the stirring rate of the second stirring and mixing process is 60r / min to 500r / min; Preferably, the stirring rate of the third stirring and mixing process is 300 r / min to 3000 r / min; Preferably, the stirring time of the third stirring and mixing process is 5 minutes to 30 minutes; Preferably, in step S5, the drying process includes heating vacuum drying or freezing freeze drying; Preferably, in the step S5, the moisture content of the nano slow-release oil-based composition is controlled to be no higher than 0.5% by the drying process; Preferably, in step S1, the weight proportions of the protein, the aqueous phase gelling agent and water are: 5 to 20 servings of protein; 0.1 to 5 parts of aqueous phase gelling agent; 350 to 850 parts of water; Preferably, in step S2, the weight proportions of linseed oil and EPA algae oil are: 20-60 parts of linseed oil; 10 to 35 parts of EPA algae oil.

7. The method for preparing the nano slow-release oil-based composition according to claim 6, characterized in that: The step S4 is to cool the oil-based coagulant and add the DHA algae oil and Haematococcus pluvialis oil in the algae oil to the water phase mixture to obtain an oil-water coagulant; and the oil-water coagulant is subjected to the third stirring and mixing treatment, emulsification treatment and high-pressure homogenization treatment to form a three-dimensional network structure with protein, phospholipids in the EPA algae oil, the water phase gelling agent and the oil phase gelling agent, and to capture and encapsulate the dispersed nano-liquid oil droplets to form a composite oil-based coagulant-emulsification system, including: The oil-based coagulant is subjected to a temperature reduction treatment to be below 70° C.; Taking DHA algae oil and Haematococcus pluvialis oil, adding them to the oil-based coagulant after the cooling treatment, and performing the second stirring and mixing treatment to obtain an oil-based coagulant-DHA algae oil mixture; Adding the oil-based coagulant-DHA algae oil mixture to the aqueous phase mixture to obtain the oil-water coagulant; The oil-water coagulant is subjected to the third stirring and mixing treatment, the emulsification treatment and the high-pressure homogenization treatment in sequence, so that the protein in the oil-water coagulant, the phospholipid in the EPA algae oil, the water phase gelling agent and the oil phase gelling agent form a three-dimensional network structure, and the dispersed nano-liquid oil droplets are captured and included to form the composite oil-based coagulant-emulsification system; Preferably, the weight ratio of the added DHA algae oil and the Haematococcus pluvialis oil to the oil-based coagulant is: (10-45): (0.2-3.5): (30-80); Preferably, the ratio of the oil-based gel-DHA algae oil mixture to the aqueous phase mixture is 1:(5-10).

8. The method for preparing the nano slow-release oil-based composition according to claim 6, characterized in that: In the step S3, the weight ratio of the added oil phase gelling agent and yeast to the oil phase mixture is (0.5-8):(0.1-3):(30-80).

9. A feeding method of the nano slow-release oil-based composition as claimed in any one of claims 1 to 5, or the nano slow-release oil-based composition prepared by the preparation method of the nano slow-release oil-based composition as claimed in any one of claims 6 to 8, characterized in that: The feeding method comprises at least one of the following methods: A. directly feeding the nano slow-release oil-based composition; B. mixing the nano slow-release oil-based composition with animal feed and then feeding the mixture to the animal; C. Covering the nano slow-release oil-based composition on the surface of the animal feed and then feeding; Wherein, the feed includes food and / or medicine; the food includes at least one of granular dry food, wet food and freeze-dried meat particles.

10. An application of the nano slow-release oil-based composition according to any one of claims 1 to 5, or the nano slow-release oil-based composition prepared by the method for preparing the nano slow-release oil-based composition according to any one of claims 6 to 8, in preparing animal health products, characterized in that: The animal health care product comprises at least one of a hair beautifying product and a weight loss product.

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