Cosmetic composition having Anti-oxidation, Anti-inflammatory and skin regeneration effects, containing mixed extract of

Abstract

The present invention discloses a cosmetic composition and, more specifically, discloses a cosmetic composition which comprises a mixed extract ultrasonically extracted from a mixture of Chinese yam, ganoderma lucidum, cornflower, and saponaria, provides antioxidant, anti-inflammatory, and skin regeneration effects, and thus can become a brand new progress on the basis of skin care products of natural origin.

Description

Technical Field

[0001] The present invention discloses a cosmetic composition, and more specifically, discloses a novel and advanced cosmetic composition that contains a mixed extract ultrasonically extracted from a mixture of Chinese yam, Ganoderma lucidum, cornflower, and Saponaria officinalis, provides antioxidant, anti-inflammatory, and skin regeneration effects, and can thus serve as the basis for a natural-origin skin care product. Background Art

[0002] The cosmetic composition industry has studied various natural ingredients in order to find ingredients that provide effects beneficial to the skin. In particular, natural-origin ingredients with antioxidant, anti-inflammatory, and skin regeneration effects have always been of concern. For example, it is known that Ganoderma lucidum provides a strong antioxidant effect, while Chinese yam contains ingredients beneficial for skin regeneration. Cornflower and Saponaria officinalis have also been reported to have the efficacy of alleviating skin inflammation and promoting skin health.

[0003] Although specific effects can be obtained when these natural raw materials are used independently, there is some lack of research on methods for properly combining them to obtain a synergistic effect. Moreover, in terms of extraction methods, existing extraction methods are accompanied by problems such as low efficiency or unstable quality. Most of the existing technologies require excessive heat or chemical treatment in the extraction method, which may cause loss of active ingredients or reduction in quality of the extract.

[0004] That is, although the existing cosmetic compositions can provide many effects after applying the natural ingredients, there is a lack of research on the proper combination or extraction method of each ingredient, which limits the cosmetic composition products that can provide high-efficiency antioxidant, anti-inflammatory, and skin regeneration effects. Many existing extraction methods may not be able to maximize the efficacy of the ingredients.

[0005] Therefore, there is an urgent need to develop a novel and advanced cosmetic composition as described below. This cosmetic composition efficiently extracts the extracts of Chinese yam, Ganoderma lucidum, cornflower, and Saponaria officinalis through ultrasonic extraction, ensuring higher efficiency than existing extraction methods, maximizing antioxidant, anti-inflammatory, and skin regeneration effects, being able to maximize the preservation of the active ingredients possessed by each original substance, and thereby maximizing the promotion of antioxidant, anti-inflammatory, and skin regeneration effects.

[0006] Prior Art Documents

[0007] Patent Documents

[0008] (Patent Document 0001) Korean Patent Publication No. 10-2007-0038922 (April 20, 2007) "Cosmetic Composition Having Antioxidant Activity" Summary of the Invention

[0009] In order to solve the problems described above, an object of the present invention is to provide a cosmetic composition which is obtained by ultrasonically extracting a mixture of the root of Chinese yam, the whole herb of Ganoderma lucidum, the flower part of Centaurea cyanus, and the whole herb containing the root of Saponaria officinalis, and which has better antioxidant, anti-inflammatory and skin regeneration effects.

[0010] To achieve the above object, the present invention discloses a cosmetic composition containing a mixed extract of Chinese yam, Ganoderma lucidum, Centaurea cyanus and Saponaria officinalis and having antioxidant, anti-inflammatory and skin regeneration effects, which includes a mixed extract obtained by ultrasonically extracting a mixture of Chinese yam, Ganoderma lucidum, Centaurea cyanus and Saponaria officinalis.

[0011] Moreover, the mixed extract is prepared as follows: the mixture is ultrasonically extracted in an extraction solvent at 30 - 50 Hz, 400 - 600 Watt, and 30 - 40 °C for 1 - 5 hours.

[0012] Moreover, the extraction solvent is a substance selected from the group consisting of water, anhydrous or hydrous lower alcohols having 1 to 4 carbon atoms, ethyl acetate, glycerol, and ethylene glycol, or a mixture of one or more of them mixed with 1,2 - hexanediol.

[0013] Moreover, the mixture is a mixture of Chinese yam, Ganoderma lucidum, Centaurea cyanus and Saponaria officinalis mixed in the same weight ratio.

[0014] Moreover, the Chinese yam is the root of Chinese yam, the Ganoderma lucidum is the whole herb of Ganoderma lucidum, the Centaurea cyanus is the flower part of Centaurea cyanus, and the Saponaria officinalis is the whole herb containing the root of Saponaria officinalis.

[0015] The cosmetic composition containing a mixed extract of Chinese yam, Ganoderma lucidum, Centaurea cyanus and Saponaria officinalis according to the present invention and having antioxidant, anti - inflammatory and skin regeneration effects includes a mixed extract obtained by ultrasonically extracting a mixture of Chinese yam, Ganoderma lucidum, Centaurea cyanus and Saponaria officinalis, thereby providing excellent antioxidant, anti - inflammatory and skin regeneration effects. Brief Description of the Drawings

[0016] Figure 1 is a flowchart showing the preparation method of the cosmetic composition of the present invention.

[0017] Figure 2 is a graph showing the experimental results of cell viability in the first experimental example.

[0018] Figure 3 is a graph showing the experimental results of antioxidant efficacy in the second experimental example.

[0019] Figure 4 is a graph showing the experimental results of antioxidant efficacy in the third experimental example.

[0020] Figure 5It is a graph showing the experimental results of the anti-inflammatory efficacy of the fourth experimental example.

[0021] Figure 6 It is a graph showing the experimental results of the moisturizing efficacy of the fifth experimental example.

[0022] Figure 7 It is a conceptual diagram showing the cell regeneration efficacy experiment of the sixth experimental example.

[0023] Symbol Explanation

[0024] S10: Mixing Step

[0025] S20: Ultrasonic Extraction Step

[0026] S30: Concentration Step

[0027] S40: Drying Step Detailed Implementation Manner

[0028] The following detailed description of the present invention is an embodiment in which the present invention can be implemented and refers to the accompanying drawings shown as examples of the embodiment. These embodiments will be described in detail in a manner that enables those skilled in the art to fully implement them. Although the various embodiments of the present invention are different from each other, it should be understood that they are not necessarily mutually exclusive. For example, the specific shapes, structures, and characteristics described in a certain embodiment can be implemented in other embodiments without departing from the spirit and scope of the present invention. Moreover, the positions or configurations of the individual elements within each described embodiment can be changed without departing from the spirit and scope of the present invention.

[0029] Therefore, the following detailed description should not be construed in a restrictive sense. As long as it can be properly described, the scope of the present invention can only be defined by all equivalent ranges equivalent to the content claimed in its claims and the appended claims. Similar symbols in the figures indicate that their functions are the same or similar in all aspects.

[0030] The terms used in the present invention have been selected as generally used terms that are currently widely used as much as possible considering their functions in the present invention. However, they may change due to factors such as the intentions or practices of those skilled in the art or the emergence of new technologies. Moreover, in specific cases, terms arbitrarily selected by the applicant are also used. At this time, their meanings will be described in detail in the corresponding invention content. Therefore, the terms used in the present invention cannot be defined solely by the term names and should be defined based on the meanings they possess and the overall content of the present invention.

[0031] When referring to a certain part of the present invention as "including" a certain element, it means that unless otherwise specifically stated, it does not exclude other elements but can also include other elements.

[0032] Hereinafter, a cosmetic composition having antioxidant, anti-inflammatory and skin regeneration effects, which contains a mixed extract of yam, Ganoderma lucidum, cornflower and Saponaria officinalis according to the present invention, will be described in detail.

[0033] The cosmetic composition having antioxidant, anti-inflammatory and skin regeneration effects, which contains a mixed extract of yam, Ganoderma lucidum, cornflower and Saponaria officinalis according to the present invention, includes a mixed extract obtained by subjecting a mixture of yam, Ganoderma lucidum, cornflower and Saponaria officinalis to ultrasonic extraction.

[0034] Here, the yam (Dioscorea Oppositifolia (Wild Yam)) is a vine-like perennial herb belonging to the Dioscoreaceae family and has thick and long tuberous roots. The pharmacological components contained in the rhizome of yam are amylase, batasin, mucin, mucilage, allantoin, etc., and it is used for the treatment of nourishing and strengthening, pulmonary tuberculosis and diabetes. The saponin components of yam are considered to be able to reduce arteriosclerosis, hypertension and the cholesterol content in the blood. Among them, dioscin and diosgenin, which are steroidal saponins, are considered to have antioxidant and antibacterial effects.

[0035] The yam of the present invention is the root of yam, and the present invention is better because it mainly uses the part containing a large amount of main components.

[0036] The Ganoderma lucidum, which is one of the ten immortals, is a mushroom used as a medicinal material with excellent effects in preventing adult diseases. It is an annual mushroom and mainly grows at the bottom end or stumps of living tree roots from summer to winter. Because of its functions such as calming the body and mind, supplementing qi and blood, and relieving cough, it is used in traditional Chinese medicine prescriptions for diseases such as neurasthenia, insomnia, dizziness, and chronic cough.

[0037] In particular, the substances contained in Ganoderma lucidum include polysaccharides, triterpenes, nucleosides, etc. These substances make macrophages and lymphocytes exposed to the Ganoderma lucidum extract secrete more immune substances (such as interferon, etc.) compared to macrophages and lymphocytes that are not exposed. Preferably, the Ganoderma lucidum of the present invention is the whole herb of Ganoderma lucidum.

[0038] The cornflower (Centaurea Cyanus) is a dicotyledonous plant of the Asteraceae family in the Campanulales order. Its origin is in eastern and southern Europe and it was previously only cultivated for ornamental purposes. Cornflower has long been closely used in people's lives and is mainly used as tea, and its fragrance and taste are faintly mellow.

[0039] When applied to the skin, cornflower has an anti-inflammatory effect of reducing inflammation and a sedative effect, and is considered helpful for calming red skin, especially effective for dry skin. Moreover, cornflower can make the skin full of vitality, and the extract of its leaves can also be used as eye drops. Preferably, the cornflower of the present invention is the flower part of cornflower, and can perform targeted extraction on the main components and the unique faint fragrance.

[0040] The English name of the soapwort (Saponaria Officinalis) is Soapwort, and its name comes from the Latin "sapo" which means soap. Crushing the leaves and roots can produce foam, and the Egyptians, Greeks, Romans, and people in the Middle Ages all used this soapwort to wash clothes. Its main components include ingredients such as triterpene, resin, mucus, phytosterol, vitamin C, and volatile oil, and contain a large amount of saponins as natural surfactant components. Preferably, the soapwort of the present invention is the whole plant including the roots of soapwort.

[0041] The mixed extract can be prepared by ultrasonic extraction of the mixture.

[0042] Specifically, the mixed extract can be prepared as follows: a mixture of yam, ganoderma lucidum, cornflower, and soapwort mixed in the same weight ratio is ultrasonically extracted in an extraction solvent at 30 - 50 Hz, 400 - 600 Watt, and 30 - 40 °C for 1 - 5 hours.

[0043] Here, the extraction solvent can be one or a mixture of two or more selected from the group consisting of water, anhydrous or hydrous lower alcohols with 1 to 4 carbon atoms, ethyl acetate, glycerol, and ethylene glycol, and a solvent mixed with 1,2 - hexanediol. Preferably, the extraction solvent is a solvent obtained by mixing 75 parts by weight of purified water, 15 parts by weight of glycerol, and 5 parts by weight of 1,2 - hexanediol.

[0044] Furthermore, preferably, the mixed extract is prepared as follows: a mixture of yam, ganoderma lucidum, cornflower, and soapwort mixed in the same weight ratio is ultrasonically extracted in an extraction solvent mixed with 75 parts by weight of purified water, 15 parts by weight of glycerol, and 5 parts by weight of 1,2 - hexanediol at 40 Hz, 500 Watt, and 40 °C for 4 hours.

[0045] At this time, preferably, the weight ratio of the mixture to the extraction solvent is 1:40. For example, the mixed extract can be prepared by ultrasonically extracting after mixing 250 g of the mixture with 9.75 kg of the extraction solvent to obtain 10 kg.

[0046] Preferably, the cosmetic composition of the present invention is completed by concentrating and drying the mixed extract after ultrasonic extraction in a manner applicable to various dosage forms. Specifically, the 10 kg of the mixed extract prepared as described above is concentrated under reduced pressure to 125 g, which is 50% by weight of the weight of the mixture, and then freeze-dried to 62.5 g, which is 50% by weight of the concentrated weight, to remove the residual solvent, thereby preparing the cosmetic composition of the present invention.

[0047] That is, preferably, in the cosmetic composition of the present invention, the mixed extract is concentrated under reduced pressure to 50% by weight of the weight of the mixture and freeze-dried until it becomes 50% by weight of the weight of the concentrate to form a powder so as to be applicable to various dosage forms.

[0048] The cosmetic composition of the present invention can be formulated into lotion, lotion, cream, foundation cream, essence, gel, mask, emulsified sunscreen, emulsified foundation, emulsified base makeup, oil powder cake foundation, two-way powder cake or powder cake, etc.

[0049] These dosage forms contain commonly used ingredients in addition to the cosmetic composition of the present invention. For example, they can contain general adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments, dyes and fragrances, as well as carriers.

[0050] For example, for ointment, cream or gel containing the cosmetic composition according to the present invention, the carrier component can be selected from one or more of animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide and then used.

[0051] The cosmetic composition of the present invention as described above can be prepared through a mixing step (step S10), an ultrasonic extraction step (step S20), a concentration step (step S30) and a drying step (step S40) as Figure 1 shown. A specific example of the preparation method is revealed through the preparation process of the following first embodiment.

[0052] The effects exerted by the cosmetic composition containing the mixed extract of Chinese yam, Ganoderma lucidum, cornflower and Saponaria officinalis according to the present invention and having antioxidant, anti-inflammatory and skin regeneration effects will be described in detail below through the following examples, comparative examples and experimental examples.

[0053] First embodiment. The cosmetic composition of the present invention

[0054] Mixing step (step S10): Dry all of the Chinese yam root, Ganoderma lucidum whole herb, cornflower, and Saponaria officinalis whole herb and cut them into 0.5 - 5 cm 3Weighing: 250 g of the mixture was prepared by filling 62.5 g each into 20-mesh extraction nets according to their respective sizes.

[0055] Ultrasonic extraction step (step S20): After pouring the extraction net containing 250 g of the mixture into 9.75 kg of extraction solvent (refined water: glycerol: 1,2-hexanediol, 75:15:5), ultrasonic extraction was performed at 40 Hz, 500 Watt, and 40 °C for 4 hours to prepare 10 kg of the mixed extract.

[0056] Concentration step (step S30): After removing the extraction net containing the mixture, vacuum concentration was carried out until the mixed extract became 125 g.

[0057] Drying step (step S40): Subsequently, freeze-drying was performed to prepare 62.5 g of the cosmetic composition of the first embodiment.

[0058] First Comparative Example. Example of hot water extraction

[0059] In the preparation method of the first embodiment, only the ultrasonic extraction step (step S20) was changed to a process of putting the extraction net containing 250 g of the mixture into 9.75 kg of refined water at 40 °C and performing hot water extraction for 4 hours to prepare 10 kg of the mixed extract, thereby preparing the cosmetic composition of the first comparative example.

[0060] Second Comparative Example. Example of changing the hot water extraction conditions

[0061] In the preparation method of the first comparative example, the hot water extraction process was changed to a process of putting the extraction net containing 250 g of the mixture into refined water at 70 °C and performing hot water extraction for 8 hours, thereby preparing the cosmetic composition of the second comparative example.

[0062] Third Comparative Example. Example of extraction with 50% aqueous ethanol

[0063] In the preparation method of the first comparative example, the hot water extraction process was changed to a process of putting the extraction net containing 250 g of the mixture into 50% aqueous ethanol at 70 °C and performing hot water extraction for 8 hours, thereby preparing the cosmetic composition of the third comparative example.

[0064] The cosmetic compositions of the first embodiment and the first to third comparative examples were mixed with refined water, and samples of various concentrations were prepared according to their respective experimental examples.

[0065] First Experimental Example. Confirmation of cell viability (MTT assay)

[0066] 1) Experimental method

[0067] The effects on cell proliferation were confirmed for the samples prepared in the first embodiment and the first to third comparative examples. The following method was adopted to conduct experiments for confirming cell proliferation and toxicity.

[0068] The cells Raw 264.7 cell (murine macrophage cell line) in culture were subcultured at 1×10 5 cells / ml into a 96-well microplate and cultured in a thermostat for 30 minutes. After adding the samples of the first embodiment and the first to third comparative examples at each concentration, they were cultured for 72 hours.

[0069] After 72 hours of culture, thiazoline blue was added and the culture was continued for 4 hours. After completely pouring out the culture medium, a reaction termination solution was poured into each well of the microplate and stirred for 5 minutes, and then the absorbance was measured at 570 nm. The control group was added with a 10% fetal bovine serum (FBS) medium according to the sample injection volume and cultured under the optimal conditions for cell growth simultaneously. The cell proliferation rate of the experimental group with the added sample was calculated by setting the cell proliferation of the control group as 100%.

[0070] The cell proliferation effect was calculated using the following Mathematical Formula 1, and the results are shown in Table 1 below and Figure 2 In Table 1 and Figure 2 In, after adding the relative cell proliferation effect calculated by Mathematical Formula 1 (for example, -3%) to the control group reference value (100%), the total cell proliferation amount compared to the control group was shown as a percentage (for example, 97%).

[0071]

Mathematical Formula 1

[0072]

[0073] 2) Experimental results

[0074]

Table 1

[0075]

[0076] As shown in Table 1, it was confirmed that the experimental results of the first embodiment, the first comparative example, and the second comparative example did not show significant cytotoxicity. However, at high concentrations, the third comparative example showed a tendency of a sharp decrease in the cell proliferation effect compared to the control group. The reason should be that the extraction solvent was changed to 50% ethanol.

[0077] Second experimental example. Confirming the antioxidant effect (DPPH free radical scavenging activity)

[0078] 1) Experimental method

[0079] The effects on free radical scavenging were confirmed for the samples prepared in the first embodiment and the first to third comparative examples. The following method was adopted to conduct experiments for confirming the effects on free radical scavenging.

[0080] Experiments were conducted using the DPPH (1,1-diphenyl-2-picrylhydrazyl) method (see Blois.M.S. Nature 181, 1190, 1958). Products from SIGMA were used for DPPH (control group) and 100 μg / ml Ascorbic Acid (positive control group). After adding 2 ml each of the first embodiment and the first to third comparative examples with different concentrations and the positive control group to 1 ml of 0.2 mM DPPH methanol solution and stirring, the reaction was carried out at room temperature for 10 minutes. Then, the absorbance was measured at 517 nm using a microplate reader. At this time, in order to compensate for the background signal, a blank test was conducted by replacing each sample with purified water. After confirming that the background signal remained constant in the blank test results, the experiments were carried out.

[0081] The free radical scavenging activity was measured using the following Mathematical Formula 2 and the results are shown in Figure 3 Table 2.

[0082]

Mathematical Formula 2

[0083]

[0084] 2) Experimental results

[0085]

Table 2

[0086]

[0087] As can be seen from Table 2, the DPPH free radical scavenging activity showed a tendency to gradually increase with the sample concentration. The first embodiment showed higher scavenging activity overall than the first to third comparative examples in all concentration ranges.

[0088] Moreover, the first embodiment showed a tendency for the scavenging activity to increase more rapidly with increasing concentration. That is, the first embodiment showed the DPPH free radical scavenging activity closest to the positive control group and exhibited excellent antioxidant activity compared to the first to third comparative examples.

[0089] Third experimental example. Confirming the antioxidant effect (ABTS free radical scavenging activity)

[0090] 1) Experimental method

[0091] The ABTS radical scavenging method was used to further analyze the antioxidant capacity. ABTS+ was formed by mixing potassium persulfate and 7 mM ABTS and using it in the dark at room temperature for about 16 hours. It was measured at an absorbance of 415 nm and diluted with ethanol to a value of 0.70 ± 0.02. After mixing 5 μl of 100 μg / ml Ascorbic Acid (positive control group) and the samples of the first example and the first to third comparative examples at various concentrations with 95 μl of the diluted ABTS solution, the absorbance was measured using a microplate reader (415 nm) after reacting for 15 minutes.

[0092] At this time, the radical scavenging effect was calculated by the above-mentioned mathematical formula 2, and the ABTS solution in the state where no sample was added was set as the control group.

[0093] The experimental results are shown in Table 3 below and Figure 4 .

[0094]

Table 3

[0095]

[0096] As can be seen from Table 3, the first example showed higher ABTS radical scavenging activity than the first to third comparative examples in all concentration ranges. That is, the first example showed excellent radical scavenging activity compared to the first to third comparative examples, and together with the second experimental example, it was confirmed again that the selection of ultrasonic extraction and extraction solvent is very important for the expression of antioxidant efficacy.

[0097] Fourth experimental example. Confirm the anti-inflammatory effect (β - hexosaminidase release)

[0098] 1) Experimental method

[0099] Samples at various concentrations were treated on the RBL - 2H3 cell line (Rat mast cell line) to exert an effect of inhibiting antigen - induced degranulation, and the anti - inflammatory effect was confirmed.

[0100] The RBL - 2H3 cell line was sub - cultured into a 24 - well plate at a rate of 2×10 5 cells per well, and each well was sensitized with 200 ng / mL of anti - DNP IgE and cultured in a 5% CO 2 medium for 12 hours.

[0101] Regarding the cell line, after washing twice with siraganian buffer, samples from the first example and the first to third comparative examples were added at each concentration, and the reaction was restarted at 37°C for 30 minutes. After treating with DBP-HAS (20 ng / mL) and inducing an allergic reaction for 30 minutes, the reaction was stopped in an ice bath for 10 minutes, then centrifuged at 12,000 rpm for 3 minutes and only the supernatant was recovered for measuring β-hexosaminidase. Regarding the measurement of β-hexosaminidase secretion inhibition, after reacting a reaction solution prepared by mixing 30 μL of the supernatant with 30 μL of 1 mM p-NAG at 37°C for 1 hour, the reaction was terminated by adding 250 mL of 0.1 M carbonate buffer, and the absorbance was measured at 405 nm using an ELISA reader (Molecular Devices, USA).

[0102] Together with the non-treated group, the control group was set as those who were treated with DBP-HAS but without adding samples.

[0103] The experimental results are shown in Table 4 below and Figure 5 .

[0104] 2) Experimental results

[0105]

Table 4

[0106]

[0107] As shown in Table 4, it can be seen that the release amounts of β-hexosaminidase in the first example and the first to third comparative examples all decreased in a concentration-dependent manner. In particular, the first example showed the lowest reduction amount in all concentration ranges, indicating excellent anti-inflammatory efficacy.

[0108] Fifth experimental example. Confirmation of moisturizing efficacy (evaluation of the production of the moisturizing factor hyaluronic acid)

[0109] 1) Experimental method

[0110] To confirm the hyaluronic acid biosynthesis effect at the cellular level for samples at each concentration, an ELISA assay was performed using an HA-ELISA kit (Echelon, USA). HaCaT cells were seeded at 2×10 5After seeding at a concentration of / well into a 6-well plate, it was stabilized for 24 hours. After washing twice with Serum-free DMEM, the medium was changed to serum-free DMEM. Each sample was treated at 5, 25, 50, 100 μg / mL, and the final concentration of DMSO was set at 0.1%. After stabilizing for 24 hours, the medium was removed and centrifuged at 15,000 rpm for 5 minutes, and the supernatant was obtained and used as a sample for ELISA. The positive control group used 1 μM ATRA.

[0111] The experimental results are as shown in Table 5 below and Figure 6 as follows.

[0112] 2) Experimental results

[0113]

Table 5

[0114]

[0115] As shown in Table 5, the first embodiment produced the most hyaluronic acid in all concentration ranges. That is, the first embodiment can provide a more enhanced moisturizing effect, and it can also be known that the cosmetic composition according to the present invention can provide an additional moisturizing effect.

[0116] Sixth experimental example. Cell regeneration (wound healing)

[0117] 1) Experimental method

[0118] This experimental example aimed to confirm the migration of keratinocytes, which is an important mechanism in wound healing. After inoculating keratinocytes (HaCaT) into a 24-well plate at a concentration of 1×10 5 cells / ml and culturing, the cells were scraped off using a 200 μl pipette tip.

[0119] After that, each sample was added to the well plate and the cell migration was measured for 36 hours.

[0120] 2) Experimental results

[0121] The experimental results are as Figure 7 shown. The cell migration and recovery of the first embodiment were the most active, and the narrowest wound site was shown after 36 hours. That is, the first embodiment provides the most excellent cell regeneration ability, and it was confirmed that the cosmetic composition of the present invention can provide a skin regeneration effect.

[0122] The foregoing has described the present invention in conjunction with the accompanying drawings, but it is merely one embodiment among various embodiments that embody the gist of the present invention. Its purpose is to enable those with ordinary knowledge in the technical field to which the present invention pertains to easily understand. Obviously, the present invention is not limited to the described embodiment. Therefore, the scope of protection of the present invention should be defined by the claims, and all technical spirits within the equivalent scope achieved by means of modification, substitution, replacement, etc. without departing from the gist of the present invention should be construed as belonging to the rights of the present invention. Moreover, some of the elements in the drawings are merely for more clearly illustrating, and their actual situations are exaggerated or shrunk excessively.

Claims

1. A cosmetic composition comprising a mixed extract of Chinese yam, ganoderma lucidum, cornflower and saponaria officinalis having antioxidant, anti-inflammatory and skin regeneration effects, characterized in that: The invention relates to a mixed extract obtained by ultrasonically extracting a mixture of Chinese yam, ganoderma lucidum, cornflower and saponaria officinalis.

2. The cosmetic composition according to claim 1, characterized in that The mixed extract is prepared by subjecting the mixture to ultrasonic extraction in an extraction solvent at 30 to 50 Hz, 400 to 600 Watt, and 30 to 40° C. for 1 to 5 hours.

3. The cosmetic composition according to claim 2, characterized in that The extraction solvent is a solvent selected from the group consisting of water, anhydrous or hydrous low-carbon alcohols with carbon numbers of 1 to 4, ethyl acetate, glycerol and ethylene glycol, or a mixture of two or more substances and 1,2-hexanediol.

4. The cosmetic composition according to claim 1, characterized in that The mixture is prepared by mixing the Chinese yam, ganoderma lucidum, cornflower and soapwort in the same weight ratio.

5. The cosmetic composition according to claim 4, characterized in that The Chinese yam is the root of Chinese yam, the Ganoderma lucidum is the whole herb of Ganoderma lucidum, the Cornflower is the flower part of Cornflower, and the Soapwort is the whole herb including the root of Soapwort.

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