Synergistic anti-inflammatory repair composition and application thereof in sensitive skin micro-ecological nursing

Abstract

The invention discloses a synergistic anti-inflammatory repair composition and application thereof in sensitive skin micro-ecological nursing, a formula comprises a scutellaria baicalensis root extract, a leontopodium leontopodum extract, a carrier, ceramide NP, panthenol and deionized water, and the application comprises the following steps: step 1, weighing raw materials; 2, preparing a water phase; step 3, premixing treatment; step 4, homogenizing and emulsifying; 5, adjusting the pH value; high-irritation components are abandoned, mild natural plant extracts are selected for replacement, the synergistic effect of the scutellaria baicalensis root extract and the leontopodium leontopodum extract is utilized, the effect is guaranteed, meanwhile, irritation to sensitive skin is greatly reduced, safety and effectiveness are both considered, damaged skin barriers can be rapidly repaired, and the skin care effect is achieved. The skin care composition can be used for skin care, skin microecology can be continuously adjusted, a long-acting protection effect on microecology disorder caused by repeated inflammation is achieved, the skin is kept in a stable and healthy state, and the problems of inflammation, irritation and long-acting repair in the sensitive skin care process are solved from the source.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and specifically to a synergistic anti-inflammatory and repair composition and its application in the microecological care of sensitive skin. Background Art

[0002] Sensitive skin is mainly caused by abnormal skin barrier function. The cutin layer of this kind of skin is relatively thin, extremely sensitive to external stimuli, and prone to inflammatory reactions, bringing many troubles to the daily life of patients. In the prior art, there are generally the following problems with cosmetics for sensitive skin care: 1. Interaction between inflammation and microecological imbalance: In the formulation design of existing cosmetics for sensitive skin care, most tend to rely on a single anti-inflammatory ingredient, such as bisabolol. However, a large number of clinical studies and academic achievements have shown that there is a close dynamic relationship between the skin microecosystem and inflammation. This single treatment strategy targeting only inflammation ignores the balance of skin flora, resulting in poor efficacy of cosmetics in improving the interaction between skin inflammation and microecological imbalance; 2. Risk of ingredient irritation: Some cosmetics for sensitive skin care contain irritating ingredients, such as high-concentration acids or alcohol. Although these ingredients show certain effects in some specific skin care scenarios, for sensitive skin, the irritation of these ingredients is too strong and is extremely likely to aggravate sensitive symptoms. Existing technical means are difficult to effectively reduce the irritation of ingredients to sensitive skin while ensuring the efficacy of cosmetics, and it is difficult to achieve the balance between efficacy and safety; 3. Insufficient long-term effectiveness of repair: Conventional barrier repair ingredients used in existing cosmetics for sensitive skin care, such as ceramide, cannot cope with the microecological disorders caused by repeated inflammation, resulting in unstable skin conditions. Although some technologies have paid attention to problems in microecological regulation or anti-inflammation, these technologies have not achieved the synergistic optimization of microecological regulation and anti-inflammation, and have not fundamentally solved the problems of interaction between inflammation and microecological imbalance, ingredient irritation, and long-term effectiveness of repair faced in the process of sensitive skin care. Summary of the Invention

[0003] The purpose of the present invention is to provide a synergistic anti-inflammatory and repair composition and its application in the microecological care of sensitive skin, so as to solve the problems raised in the above background art.

[0004] To achieve the above purpose, the present invention provides the following technical solution: A synergistic anti-inflammatory and repair composition, the formula includes: Scutellaria baicalensis root extract, Leontopodium alpinum extract, carrier, ceramide NP, panthenol and deionized water. The mass percentage contents of each component are respectively: 1.5 - 3% of Scutellaria baicalensis root extract, 3.5 - 5% of Leontopodium alpinum extract, 8 - 18% of carrier, 0.1 - 0.5% of ceramide NP, and 0.5 - 3.0% of panthenol, and the balance is deionized water.

[0005] Preferably, the mass percentage contents of the respective components are as follows: 2.4% of Scutellaria baicalensis root extract, 4.8% of Leontopodium alpinum extract, 13% of a carrier, 0.3% of ceramide NP, 2% of panthenol, and 77.5% of deionized water.

[0006] Preferably, the carrier is one or a combination of glycerol, 1,2-pentanediol, and sodium hyaluronate.

[0007] Preferably, in the Scutellaria baicalensis root extract, the content of baicalin is ≥80%, and in the Leontopodium alpinum extract, the content of leontopodic acid is ≥5%.

[0008] The application of the synergistic anti-inflammatory and repair composition in the microecological care of sensitive skin includes Step 1, weighing raw materials; Step 2, preparing the aqueous phase; Step 3, pre-mixing treatment; Step 4, homogenizing and emulsifying; Step 5, adjusting the pH.

[0009] Among them, in the above Step 1, based on the sum of the mass percentage contents of the respective components being 1, 2.4% of Scutellaria baicalensis root extract, 4.8% of Leontopodium alpinum extract, 8% of glycerol, 5% of 1,2-pentanediol, 0.3% of ceramide NP, 2% of panthenol, and 77.5% of deionized water are weighed according to the formula ratio.

[0010] Among them, in the above Step 2, the Scutellaria baicalensis root extract and glycerol weighed in Step 1 are added to deionized water and stirred until dissolved to obtain the aqueous phase.

[0011] Among them, in the above Step 3, the Leontopodium alpinum extract and 1,2-pentanediol weighed in Step 1 are ultrasonically dispersed and then added to the aqueous phase obtained in Step 2, and after stirring evenly, a mixture is obtained.

[0012] Among them, in the above Step 4, the mixture obtained in Step 3 is transferred to a homogenizing emulsifier for homogenizing and emulsifying. After emulsification is completed, an emulsion is obtained. Through a circulating cooling water system, the emulsion is cooled to below 40°C, and then ceramide NP and panthenol are added to the cooled emulsion.

[0013] Among them, in the above Step 5, the emulsion prepared in Step 4 is taken, and the pH is adjusted to 5.0 - 5.5 using a pH regulator. Then the emulsion is filtered through a 0.22 μm microporous filter membrane, and finally, the filtrate is filled into a sterilized packaging container using a sterile filling device to complete the preparation of the cosmetic.

[0014] Preferably, in the above Step 2, the temperature of the deionized water is 60 - 65°C.

[0015] Preferably, in the above Step 2, the stirring and dissolving operation is carried out using a magnetic stirrer, the stirring speed is 300 - 400 r / min, and the stirring time is 30 - 40 min.

[0016] Preferably, in the third step, the ultrasonic power for ultrasonic dispersion is 200 - 300 W, the ultrasonic frequency is 40 kHz, the ultrasonic dispersion time is 20 - 30 min, the stirring speed is 200 - 300 r / min, and the stirring time is 15 - 20 min.

[0017] Preferably, in the fourth step, the homogenization speed for homogenization emulsification is 2000 - 4000 rpm, and the emulsification time is 5 - 10 min.

[0018] Preferably, in the fourth step, continuous stirring is carried out during the addition of ceramide NP and panthenol, the stirring speed is 100 - 200 r / min, and the stirring time is 10 - 15 min.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows: The present invention abandons highly irritating components and selects mild natural plant extracts to replace them. By utilizing the synergistic effect of Scutellaria baicalensis root extract and Leontopodium alpinum extract, while ensuring the efficacy, it greatly reduces the irritation to sensitive skin, taking into account both safety and effectiveness. It can not only quickly repair the damaged skin barrier, but also continuously regulate the skin microecology, play a long-term protective role against the microecological disorders caused by repeated inflammation, keep the skin in a stable and healthy state, and fundamentally solve the problems of inflammation, irritation, and long-term repair faced in the care of sensitive skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is the application flow chart of the present invention;

[0021] Figure 2 is the bar chart of Shannon index of the compound group and the single-component group;

[0022] Figure 3 is the bar chart of the proportion of Staphylococcus epidermidis in the compound group and the single-component group;

[0023] Figure 4 is the line chart of the change of TEWL value with time;

[0024] Figure 5 is the line chart of the change of erythema index with time;

[0025] Figure 6 is the line chart of the change of stinging sensation score with time. DETAILED DESCRIPTION OF THE INVENTION

[0026] The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0027] Please refer to the attached Figure 1-6 , a technical solution provided by the present invention:

[0028] Example 1:

[0029] A co - anti - inflammatory and repair composition, the formula includes: Scutellaria baicalensis root extract, Leontopodium alpinum extract, glycerol, 1,2 - pentanediol, ceramide NP, panthenol and deionized water. The mass percentage contents of each component are respectively: 2.4% of Scutellaria baicalensis root extract, 4.8% of Leontopodium alpinum extract, 8% of glycerol, 5% of 1,2 - pentanediol, 0.3% of ceramide NP, 2% of panthenol and 77.5% of deionized water. In the Scutellaria baicalensis root extract, the content of baicalin is ≥80%, and in the Leontopodium alpinum extract, the content of leontopodic acid is ≥5%.

[0030] The application of the co - anti - inflammatory and repair composition in the micro - ecological care of sensitive skin, including step one, raw material weighing; step two, aqueous phase preparation; step three, premixing treatment; step four, homogenization and emulsification; step five, pH adjustment;

[0031] Among them, in the above step one, based on the sum of the mass percentage contents of each component being 1, weigh 2.4% of Scutellaria baicalensis root extract, 4.8% of Leontopodium alpinum extract, 8% of glycerol, 5% of 1,2 - pentanediol, 0.3% of ceramide NP, 2% of panthenol and 77.5% of deionized water according to the formula ratio;

[0032] Among them, in the above step two, add the Scutellaria baicalensis root extract and glycerol weighed in step one into deionized water at a temperature of 60 - 65°C, and use a magnetic stirrer to stir at 300 - 400 r / min for 30 - 40 min to obtain an aqueous phase;

[0033] Among them, in the above step three, ultrasonically disperse the Leontopodium alpinum extract and 1,2 - pentanediol weighed in step one. The ultrasonic power of ultrasonic dispersion is 200 - 300 W, the ultrasonic frequency is 40 kHz, and the ultrasonic dispersion time is 20 - 30 min. Then add it to the aqueous phase obtained in step two and stir at 200 - 300 r / min for 15 - 20 min to obtain a mixture;

[0034] Among them, in the above step four, transfer the mixture obtained in step three to a homogenizing emulsifier, homogenize and emulsify at 2000 - 4000 rpm for 5 - 10 min. After emulsification is completed, obtain an emulsion. Cool the emulsion to below 40°C through a circulating cooling water system, and then add ceramide NP and panthenol to the cooled emulsion, and continuously stir during the adding process. The stirring speed is 100 - 200 r / min, and the stirring time is 10 - 15 min;

[0035] In the above step 5, take the emulsion prepared in step 4, adjust the pH to 5.0 - 5.5 using a pH regulator, then filter the emulsion through a 0.22 μm microporous membrane, and finally fill the filtrate into a sterilized packaging container using a sterile filling device to complete the preparation of the cosmetic.

[0036] Example 2:

[0037] Referring to the formulation and application of Example 1, only change the mass percentage content of each component to: 1.5% Scutellaria baicalensis root extract, 5% Leontopodium alpinum extract, 8% glycerol, 5% 1,2 - pentanediol, 0.3% ceramide NP, 2% panthenol, and 78.2% deionized water.

[0038] Example 3:

[0039] Referring to the formulation and application of Example 1, only change the mass percentage content of each component to: 3% Scutellaria baicalensis root extract, 3.5% Leontopodium alpinum extract, 8% glycerol, 5% 1,2 - pentanediol, 0.3% ceramide NP, 2% panthenol, and 78.2% deionized water.

[0040] Comparative Example 1:

[0041] Referring to the formulation and application of Example 1, only change the mass percentage content of each component to: 4.8% Leontopodium alpinum extract, 8% glycerol, 5% 1,2 - pentanediol, 0.3% ceramide NP, 2% panthenol, and 79.9% deionized water.

[0042] Comparative Example 2:

[0043] Referring to the formulation and application of Example 1, only change the mass percentage content of each component to: 2.4% Scutellaria baicalensis root extract, 8% glycerol, 5% 1,2 - pentanediol, 0.3% ceramide NP, 2% panthenol, and 82.3% deionized water.

[0044] Experimental Example 1:

[0045] To verify the effect of the present invention on skin microecology, experiments were carried out using an EpiDerm TM skin model. The experimental subjects were divided into a compound group and single - component groups. The compound group used the cosmetic prepared in Example 1; single - component group A used the cosmetic prepared in Comparative Example 1, and single - component group B used the cosmetic prepared in Comparative Example 2. Each group had 10 parallel samples. Under a sterile environment, the cosmetics were evenly applied to the surface of the EpiDerm TM model, and the application amount was 0.1 g / cm 2, sample collection is carried out once every 24 hours. The microbial community structure of the sample is analyzed using high-throughput sequencing technology, and the Shannon index is calculated. At the same time, the proportion of Staphylococcus epidermidis is determined by the plate counting method; through experimental determination, the Shannon index of the microbial community in the compound group reaches 3.8 ± 0.5, which is significantly improved compared with the single-component group, indicating that the compound cosmetics can significantly increase the diversity of skin microbiota. At the same time, the proportion of Staphylococcus epidermidis in the compound group increases to 68 ± 7%, which is significantly higher than that in the single-component group, indicating that the compound cosmetics can effectively promote the growth of beneficial bacteria and optimize the skin microecological environment.

[0046] Experimental Example 2:

[0047] Thirty subjects with sensitive skin were recruited to participate in a clinical trial, aged between 20 and 45 years old. The subjects used the cosmetics containing various extracts morning and evening for 28 days. Before the start of the experiment, on the 7th day, 14th day, 21st day, and 28th day, the subjects were measured for erythema index, transepidermal water loss (TEWL), and stinging sensation score; the specific determination of the erythema index was as follows: a skin colorimeter was used to measure the erythema index of specific areas of the subjects' faces, and each measurement data was recorded; the specific determination of TEWL was as follows: a transepidermal water loss tester was used to measure the TEWL value in the same facial area, and the measurement results were recorded; the specific stinging sensation score was as follows: the subjects were asked to score the stinging sensation after using the cosmetics according to their own feelings, with the scoring range from 0 to 10, 0 indicating no stinging sensation, and 10 indicating a strong stinging sensation; through experimental determination, after 28 days of use by 30 subjects with sensitive skin, the erythema index decreased by 48 ± 8%, indicating that the cosmetics can effectively reduce skin erythema and relieve skin inflammation; the TEWL decreased by 38 ± 6%, indicating that the cosmetics help to enhance the skin barrier function and reduce water loss; the stinging sensation score was always lower than 4, proving that the cosmetics have high safety and will not cause obvious irritation to sensitive skin.

[0048] The formula components of each example and comparative example are compared as follows in the table:

[0049]

[0050]

[0051] Based on the above, the advantages of the present invention are as follows. The Scutellaria baicalensis root extract added in the present invention contains high-purity baicalin. By inhibiting the NF-κB pathway, it reduces the release of inflammatory factors such as IL-6, and at the same time enhances the activity of antioxidant enzymes to scavenge free radicals. The Leontopodium alpinum extract is rich in leontopodic acid, which can regulate the skin flora structure, promote the proliferation of beneficial bacteria, inhibit the adhesion of pathogenic bacteria, and relieve itching by inhibiting the release of histamine. The two regulate the Th1 / Th2 immune balance, synergistically enhance the flora diversity (Shannon index ≥ 3.5), reduce IL-6 to ≤ 12 pg / mL, and repair the barrier (TEWL reduction ≥ 35%), achieving multi-dimensional care for sensitive skin. Experiments show that the compounding effect is 50-70% higher than that of a single ingredient, and it is mild and non-irritating. This composition can be widely applied to soothing and repairing cosmetics.

[0052] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be regarded as limiting the claims involved.

Claims

1. A synergistic anti-inflammatory repair composition, the formula comprising: Scutellaria root extract, leontopodium extract, carrier, ceramide NP, panthenol and deionized water, characterized in that the mass percentage content of each component is: 1.5-3% of scutellaria root extract, 3.5-5% of leontopodium extract, 8-18% of carrier, 0.1-0.5% of ceramide NP and 0.5-3.0% of panthenol, and the balance is deionized water.

2. The synergistic anti-inflammatory repair composition according to claim 1, characterized in that: The mass percentage contents of the components are: 2.4% of scutellaria root extract, 4.8% of leontopodium glutamate extract, 13% of carrier, 0.3% of ceramide NP, 2% of panthenol and 77.5% of deionized water.

3. The synergistic anti-inflammatory repair composition according to claim 1, characterized in that: The carrier is one or more combinations of glycerol, 1,2-pentanediol, and sodium hyaluronate.

4. The synergistic anti-inflammatory repair composition according to claim 1, characterized in that: The content of baicalin in the scutellaria root extract is ≥80%, and the content of leontopodium glutamate in the leontopodium extract is ≥5%.

5. The application of the synergistic anti-inflammatory repair composition in the microecological care of sensitive skin comprises the steps of: weighing the raw materials; preparing the aqueous phase; premixing; homogenizing and emulsifying; and adjusting the pH; characterized in that: In the above step 1, based on the sum of the mass percentages of each component as 1, 2.4% of Scutellaria baicalensis root extract, 4.8% of Leontopodium edelweiss extract, 8% of glycerol, 5% of 1,2-pentanediol, 0.3% of ceramide NP, 2% of panthenol and 77.5% of deionized water were weighed according to the formula ratio; In the above step 2, the scutellaria root extract and glycerin weighed in step 1 are added into deionized water, stirred and dissolved to obtain an aqueous phase; In the above step 3, the leontopodium extract and 1,2-pentanediol weighed in step 1 are ultrasonically dispersed, and then added to the aqueous phase obtained in step 2, and stirred to obtain a mixture; In the above step 4, the mixture obtained in step 3 is transferred to a homogenizer for homogenization and emulsification, and an emulsion is obtained after the emulsification is completed. The emulsion is cooled to below 40° C. through a circulating cooling water system, and ceramide NP and panthenol are added to the cooled emulsion; In the above step 5, the emulsion prepared in step 4 is taken, the pH is adjusted to 5.0-5.5 using a pH adjuster, and then the emulsion is filtered through a 0.22 μm microporous filter membrane, and finally the filtrate is filled into a sterilized packaging container using a sterile filling device to complete the preparation of the cosmetics.

6. Use of the synergistic anti-inflammatory repair composition according to claim 5 in microecological care of sensitive skin, characterized in that: In the step 2, the temperature of the deionized water is 60-65°C.

7. Use of the synergistic anti-inflammatory repair composition according to claim 5 in microecological care of sensitive skin, characterized in that: In the step 2, a magnetic stirrer is used for the stirring and dissolving operation, with a stirring speed of 300-400 r / min and a stirring time of 30-40 min.

8. Use of the synergistic anti-inflammatory repair composition according to claim 5 in microecological care of sensitive skin, characterized in that: In the step three, the ultrasonic power of the ultrasonic dispersion is 200-300 W, the ultrasonic frequency is 40 kHz, the ultrasonic dispersion time is 20-30 min, the stirring speed is 200-300 r / min, and the stirring time is 15-20 min.

9. Use of the synergistic anti-inflammatory repair composition according to claim 5 in microecological care of sensitive skin, characterized in that: In the step 4, the homogenization speed of the homogenization emulsification is 2000-4000 rpm, and the emulsification time is 5-10 min.

10. Use of the synergistic anti-inflammatory repair composition according to claim 5 in microecological care of sensitive skin, characterized in that: In the step 4, stirring is continued during the process of adding ceramide NP and panthenol, the stirring speed is 100-200 r / min, and the stirring time is 10-15 min.

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