Probiotic shampoo capable of regulating scalp micro-ecology and preparation method thereof

Abstract

The invention relates to probiotic shampoo capable of regulating scalp micro-ecology and a preparation method of the probiotic shampoo, and belongs to the technical field of cosmetics. The probiotic shampoo with the scalp micro-ecology adjusting function is prepared from the following components in parts by weight: 12 to 42 parts of a surfactant, 1 to 17 parts of a hair conditioning component, 0.35 to 13 parts of a probiotic composition, 1 to 6 parts of a stable thickening agent, 0.06 to 0.12 part of a preservative, 1 to 6 parts of an appearance adjusting agent, 0.05 to 1 part of a pH (Potential of Hydrogen) adjusting agent, 0.5 to 2 parts of an aromatic agent and 40 to 65 parts of water, the probiotic composition is prepared from probiotics, prebiotics and probiotics. Through cooperative use of probiotics, prebiotics and probiotics, the probiotic shampoo capable of regulating scalp micro-ecology is prepared, the scalp micro-ecology can be greatly improved, the transdermal water loss rate of scalp can be improved, grease secretion can be controlled, dandruff can be reduced, pruritus can be improved, and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a probiotic shampoo with the function of regulating scalp microecology and a preparation method thereof. Background Art

[0002] Dandruff, also known as pityriasis capitis, is a common skin disease. It is a normal physiological metabolic phenomenon. With the in-depth research, more and more evidence shows that dandruff is an abnormal metabolic process of the epidermal stratum corneum and there are subclinical inflammatory changes. The causes of dandruff are very complex, involving microbial and non-microbial factors. Currently, there are mainly three hypotheses about the etiology of dandruff: microbial factors, skin barrier changes, and individual susceptibility factors. Microorganisms colonized on the skin surface can damage the skin barrier through various ways, leading to the production of dandruff, and the change of the barrier will also affect the colonization of microorganisms. The two jointly participate in the pathogenesis of dandruff. Long-term research has shown that the colonization of Malassezia is an important cause of dandruff. Therefore, by regulating the balance of scalp microecology and maintaining a healthy scalp microecology, it is possible to reduce scalp bacterial and fungal infections, enhance the resistance of the scalp barrier to bacterial and fungal invasion, further degrade excess scalp oil, and improve seborrheic dermatitis.

[0003] Currently, most of the anti-dandruff cosmetics on the market achieve the purpose of anti-dandruff by adding antifungal drugs as the main active ingredients to reduce the growth of scalp microorganisms. The use of these antifungal drugs does have a rapid and efficient effect on the removal of dandruff, but it will cause irritation and damage to the skin. Commonly used anti-dandruff agents include sulfur, salicylic acid, selenium disulfide, etc. Salicylic acid does not have obvious antifungal efficacy, has poor anti-dandruff effect and strong irritation, and is easy to damage the skin; the anti-dandruff shampoo with selenium disulfide as the main active ingredient not only has strong irritation, but also makes the hair dry and astringent after use. If it is not rinsed clean, the hair may have a discoloration phenomenon, and a small number of users may have hair loss, and the selenium disulfide preparation itself also has an unpleasant smell. Later, it was found that compounds such as zinc pyrithione and clotrimazole have good effects on improving dandruff, but these cannot directly affect the water retention of the scalp and the content of lipids, and long-term use of such anti-dandruff shampoos will cause serious disorders of the scalp flora.

[0004] Due to the fast pace of life and the frequent use of various shampoo bactericides, the microecology of the scalp flora is out of balance, resulting in problems such as dandruff, itching, swelling, and inflammation, and generating high drug resistance.

[0005] In recent years, in addition to using antifungal drugs for treating dandruff, the method of adding probiotic agents can also be adopted. Currently, there are already products that directly apply probiotics to shampoos. Chinese Patent CN 113413357A discloses a probiotic composition and its application. The probiotic composition includes: 0.1 - 2 parts of lactic acid bacteria powder, 0.1 - 10 parts of lactic acid bacteria fermentation lysate, 0.1 - 5 parts of plant extract, 0.1 - 2 parts of hydrolyzed corn starch, and 81 - 99.6 parts of water. The probiotic combination of this application can produce a large amount of organic acids, lower the pH value of the environment, inhibit the growth and reproduction of pathogenic bacteria, and can also produce hydrogen peroxide, antimicrobial peptides, and bacteriocins, which have an obvious inhibitory effect on pathogenic bacteria. At the same time, it can improve the immune function of the body, activate macrophages, and maintain local anti-infection ability, which has very important value for improving the scalp and skin microenvironment, controlling the growth of skin pathogenic bacteria, and improving scalp and skin itching, swelling and other inflammations. However, this application only contains probiotics, and the effect on regulating the scalp microecology and inflammatory response is not obvious. Chinese Patent CN 113018246A discloses a probiotic shampoo with the effect of relieving scalp inflammation and its preparation method; in the shampoo, by adding probiotics and prebiotics, the beneficial bacteria in the hair cortex layer are increased, the microecology is balanced, and the scalp inflammation can be effectively relieved; by adding plant extracts and mixing with probiotic components, the microecology of the hair cortex layer can be balanced, damaged hair can be repaired, and the damage of free radicals to hair can be reduced. Although this method has a good effect on balancing the microecology and anti-inflammation, the moisturizing effect is not good.

[0006] Probiotics refer to the metabolites secreted by the metabolic activities of live bacteria or the soluble factors released after the death and lysis of bacteria, and can have beneficial effects on the host. Probiotics play a synergistic role through the respective advantages of probiotics and prebiotics, and can achieve the effect of 1 + 1 + 1 > 3. It can not only optimize the skin microflora, but also strengthen the skin microbial barrier, and has an obvious effect on restructuring and smoothing the skin surface. Therefore, developing a shampoo containing probiotics, prebiotics, and probiotics can not only better regulate the balance of the scalp microecology, maintain scalp health, achieve reducing scalp bacterial infections, but also enhance the scalp barrier, further degrade the excess oil on the scalp, improve seborrheic dermatitis and solve other scalp problems. Summary of the Invention

[0007] To solve the above technical problems, the present invention provides a probiotic shampoo with the function of regulating the scalp microecology and its preparation method. By using probiotics, prebiotics, and probiotics in combination, the present invention prepares a probiotic shampoo with the function of regulating the scalp microecology, which can greatly improve the scalp microecology, improve the transdermal water loss rate of the scalp, control oil secretion, reduce dandruff, and improve itching and other conditions.

[0008] The present invention is realized through the following technical solutions:

[0009] The first object of the present invention is to provide a probiotic shampoo with the function of regulating scalp microecology. Calculated by weight parts, it includes the following components: 12 parts - 42 parts of surfactant, 1 part - 17 parts of hair conditioning ingredient, 0.35 part - 13 parts of probiotic composition, 1 part - 6 parts of stabilizing thickener, 0.06 part - 0.12 part of preservative, 1 part - 6 parts of appearance regulator, 0.05 part - 1 part of pH regulator, 0.5 part - 2 parts of fragrance, and 40 parts - 65 parts of water; the probiotic composition includes probiotics, prebiotics and postbiotics.

[0010] In one embodiment of the present invention, calculated by weight parts, the probiotic composition includes 0.11 part - 4 parts of probiotics, 0.11 part - 3 parts of prebiotics and 0.13 part - 6 parts of postbiotics.

[0011] In one embodiment of the present invention, the surfactant is one or more of anionic surfactant, cationic surfactant, amphoteric surfactant and nonionic surfactant.

[0012] In one embodiment of the present invention, calculated by weight parts, the surfactant includes 11 parts - 30 parts of anionic surfactant, 0.2 part - 2 parts of cationic surfactant, 0.5 part - 6 parts of amphoteric surfactant and 1 part - 4 parts of nonionic surfactant.

[0013] In one embodiment of the present invention, the hair conditioning ingredient is selected from one or more of polydimethylsiloxanol, polydimethylsiloxane, hydrogenated starch hydrolyzate, panthenol, panthenol ethyl ether and hydrolyzed vegetable protein.

[0014] In one embodiment of the present invention, the stabilizing thickener is selected from acrylic (ester) copolymer and / or sodium chloride.

[0015] In one embodiment of the present invention, the preservative is selected from one or more of Kathon, hexamidine di (hydroxyethyl sulfonate) salt, sodium benzoate and phenoxyethanol.

[0016] In one embodiment of the present invention, the appearance regulator is ethylene glycol distearate and / or pigment.

[0017] In one embodiment of the present invention, the pH regulator is selected from one or more of citric acid, lactic acid, sodium hydroxide, triethanolamine and arginine; the fragrance is selected from essence or natural essential oil.

[0018] The second object of the present invention is to provide a preparation method of the probiotic shampoo, including the following steps:

[0019] S1. Add a cationic surfactant to water, stir to dissolve evenly, add an anionic surfactant, heat to 80°C - 85°C to dissolve, then add an amphoteric surfactant, a non-ionic surfactant and an appearance regulator, stir to dissolve evenly, and keep stirring for 20 min - 30 min;

[0020] S2. Cool the solution obtained in step S1 to 60°C - 65°C, stir to dissolve, and add a stabilizing thickener; cool to 50°C - 55°C and stir to cool until obvious pearlescence appears;

[0021] S3. After the pearlescence is completely formed, add hair conditioning ingredients and stir evenly; continue to add a fragrance, a preservative, a probiotic composition and an appearance regulator, and stir evenly;

[0022] S4. Use a pH regulator to adjust the pH value of the solution prepared in step S3 to 5.0 - 6.5, and adjust the viscosity to 3000 cps - 15000 cps to prepare the probiotic shampoo with the function of regulating scalp microecology.

[0023] The above technical solutions of the present invention have the following advantages compared with the prior art:

[0024] (1) The present invention adds three probiotic compositions of probiotics, prebiotics and postbiotics to the shampoo. Through the synergistic effect of the three of probiotics, prebiotics and postbiotics, the effect of 1 + 1 + 1 > 3 can be achieved. It can not only increase the beneficial bacteria in the scalp cortex layer, balance the scalp microecology, inhibit the growth and reproduction of harmful bacteria, but also produce rich nutrients, promote the absorption and utilization of the scalp, and can more effectively relieve scalp inflammation and inhibit scalp oil secretion;

[0025] (2) The present invention achieves the effects of repairing damaged hair and enhancing the water-locking ability of the scalp by compounding ingredients such as hydrolyzed plant protein, hydrolyzed starch, and vitamin combination. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to make the content of the present invention easier to be clearly understood, the following further detailed description of the present invention is made according to the specific embodiments of the present invention in conjunction with the drawings, where

[0027] Figure 1 is the change trend of the water content in the stratum corneum in the test examples of the present invention;

[0028] Among them, the significance marking method: "n.s." means no statistical difference, p > 0.05; "*" means significant difference, 0.01 ≤ p < 0.05, "**" means significant difference, 0.001 ≤ p < 0.01; "***" means significant difference, p < 0.001;

[0029] Figure 2is the difference in stratum corneum water content in the test examples of the present invention;

[0030] Figure 3 is the change rate of stratum corneum water content in the test examples of the present invention;

[0031] Figure 4 is the change trend of transdermal water loss in the test examples of the present invention;

[0032] Among them, the significance annotation method: "n.s." indicates no statistical difference, p > 0.05; "*" indicates significant difference, 0.01 ≤ p < 0.05, "**" indicates significant difference, 0.001 ≤ p < 0.01; "***" indicates significant difference, p < 0.001;

[0033] Figure 5 is the difference in transdermal water loss in the test examples of the present invention;

[0034] Figure 6 is the change rate of transdermal water loss in the test examples of the present invention;

[0035] Figure 7 is the change trend of skin oil content in the test examples of the present invention;

[0036] Among them, the significance annotation method: "n.s." indicates no statistical difference, p > 0.05; "*" indicates significant difference, 0.01 ≤ p < 0.05, "**" indicates significant difference, 0.001 ≤ p < 0.01; "***" indicates significant difference, p < 0.001;

[0037] Figure 8 is the difference in skin oil content in the test examples of the present invention;

[0038] Figure 9 is the change rate of skin oil content in the test examples of the present invention;

[0039] Figure 10 is the change trend of dandruff quantity in the test examples of the present invention;

[0040] Among them, the significance annotation method: "n.s." indicates no statistical difference, p > 0.05; "*" indicates significant difference, 0.01 ≤ p < 0.05, "**" indicates significant difference, 0.001 ≤ p < 0.01; "***" indicates significant difference, p < 0.001;

[0041] Figure 11 is the difference in dandruff quantity in the test examples of the present invention;

[0042] Figure 12 is the change rate of dandruff quantity in the test examples of the present invention;

[0043] Figure 13 It is an effective example of Subject No. 003 in the test examples of the present invention;

[0044] Figure 14 It is an effective example of Subject No. 005 in the test examples of the present invention;

[0045] Figure 15 It is an effective example of Subject No. 024 in the test examples of the present invention. Detailed implementation manners

[0046] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments, so that those skilled in the art can better understand the present invention and be able to implement it, but the exemplified embodiments are not intended to limit the present invention.

[0047] The detection method involved in the present invention is as follows: Select Chinese healthy subjects with neutral or oily scalps, dandruff and mild scalp itching symptoms, and an age range of 25 to 55 years old. Analyze the dandruff count before the subjects use the test product (0d), 14 days after using the product (14d), and 28 days after using the product (28d). Measure the moisture content of the scalp cutin layer, the transdermal water loss, and the skin oil content by an instrument, and the subjects conduct self-evaluation according to a questionnaire. The data results and evaluation results before and after product use are compared by statistical test methods to determine whether there are statistical differences.

[0048] Detection site: Scalp.

[0049] Detection indicators

[0050] 1. Efficacy detection

[0051] (1) Moisture content of the cutin layer

[0052] Measuring instrument: Moisture content tester of the cutin layer MoistureSC (Delfin Technologies, Finland).

[0053] Measurement requirement: Take the average value after measuring 3 times.

[0054] Measurement area: Scalp.

[0055] Parameter explanation: The higher the value, the higher the water content of the skin cutin layer. Unit: Dimensionless.

[0056] Result determination: After the product is used, the moisture content of the cutin layer in the scalp area is significantly higher than that before use, indicating that the test sample has the effect of moisturizing.

[0057] (2) Transdermal water loss

[0058] Measuring instrument: Skin Surface Moisture Loss Tester Tewameter TM 300 (Courage&Khazaka, Germany).

[0059] Measurement requirement: Take the average value after testing 3 times.

[0060] Measurement area: Scalp.

[0061] Parameter explanation: The lower the value, the less the transdermal water loss. Unit: g / (h·m 2 )

[0062] Result determination: After using the product, if the transdermal water loss (TEWL) in the scalp area is significantly lower than that before use, it indicates that the tested product has the effect of repairing the barrier.

[0063] (3) Skin oil content

[0064] Measuring instrument: Skin Oil Content Meter Sebumeter SM 815 (Courage&Khazaka, Germany).

[0065] Measurement requirement: Test once.

[0066] Measurement area: Scalp.

[0067] Parameter explanation: The lower the value, the lower the skin oil content. Unit: μg / cm 2 .

[0068] Result determination: After using the product, if the skin oil content in the scalp area is significantly lower than that before use, it indicates that the tested product has the effect of controlling oil.

[0069] (4) Dandruff quantity

[0070] Measuring instrument: DandruffMeter DA 20 dandruff tester.

[0071] Measurement requirement: Collect an image once and analyze the image once.

[0072] Measurement area: Scalp.

[0073] Parameter explanation: Dandruff quantity, the smaller the value, the less the dandruff quantity.

[0074] Result determination: After using the product, if the dandruff quantity in the scalp area is significantly lower than that before use, it indicates that the tested sample has the effect of removing dandruff.

[0075] 2. Efficacy and safety evaluation

[0076] The methods for efficacy and safety evaluation are shown in Table 1.

[0077] Table 1 Efficacy evaluation table

[0078]

[0079] In the embodiments of the present invention, the probiotics used were purchased from Centdega (Guangzhou) Trading Co., Ltd. and Jingyue Biotechnology Co., Ltd., with the product numbers OPTIMEALTH 10W and lactic acid bacteria powder SCP-2; the prebiotics were purchased from Centdega (Guangzhou) Trading Co., Ltd., with the product number FLORESTORE; the synbiotics were purchased from SOLABIA Group, with the product number ECOSKIN; unless otherwise specified, the substances used in the embodiments of the present invention are all commercially available.

[0080] Example 1

[0081] This example provides a probiotic shampoo with the function of regulating scalp microecology. By mass, it includes the following components:

[0082] Table 2

[0083]

[0084]

[0085] The preparation method of this example includes the following steps:

[0086] S1. Stir and dissolve polyquaternium-10 evenly in water, add guar gum hydroxypropyltrimethyl ammonium chloride, and stir to dissolve; add cocamidopropyl betaine, and stir to dissolve evenly; after adding sodium lauryl polyether sulfate, heat to 80°C - 85°C, and continue to stir to dissolve; at 80°C - 85°C, sequentially add sodium methyl cocoyl taurate, sodium cocoyl aminopropionate, cocamide MEA, hydroxypropyl methylcellulose, ethylene glycol distearate, and stir to dissolve completely, and keep stirring for 20 min - 30 min;

[0087] S2. Slowly cool down to 60°C - 65°C, add acrylate copolymer, and stir to dissolve; cool down to 50°C - 55°C, add sodium chloride, and stir and cool down until obvious pearlescence appears;

[0088] S3. After the pearlescence is complete, add poly dimethicone alcohol and polydimethylsiloxane, and stir evenly; continue to add essence, preservative, panthenol, hydrogenated starch hydrolysate, panthenol ethyl ether, hydrolyzed vegetable protein, probiotic composition, pigment, and stir evenly;

[0089] S4. Take a sample for testing, adjust the pH to 5.0 - 6.5, and then adjust the viscosity to 3000 cps - 15000 cps.

[0090] Example 2

[0091] This embodiment provides a probiotic shampoo for regulating scalp microecology, which comprises the following components by mass fraction:

[0092] Table 3

[0093]

[0094]

[0095]

[0096] The preparation method of this embodiment comprises the following steps:

[0097] S1. Stir and dissolve polyquaternium-10 evenly in water, add guar gum hydroxypropyltrimethyl ammonium chloride, and stir to dissolve; add cocamidopropyl betaine, and stir to dissolve evenly; after adding sodium lauryl polyether sulfate, heat to 80°C - 85°C, and continue to stir to dissolve; at 80°C - 85°C, add sodium methyl cocoyl taurate, sodium cocoyl aminopropionate, coconut oil amide methyl MEA, hydroxypropyl methyl cellulose, ethylene glycol distearate in sequence, stir to dissolve completely, and keep stirring for 20 min - 30 min;

[0098] S2. Slowly cool down to 60°C - 65°C, add acrylic (ester) copolymer, and stir to dissolve; cool down to 50°C - 55°C, add sodium chloride, and stir and cool down until obvious pearlescence appears;

[0099] S3. After the pearlescence is completely formed, add dimethicone alcohol and dimethicone, and stir evenly; continue to add essence, preservative, panthenol, hydrogenated starch hydrolysate, panthenol ethyl ether, hydrolyzed vegetable protein, probiotic composition, pigment, and stir evenly;

[0100] S4. Take samples for testing, adjust the pH to 5.0 - 6.5, and then adjust the viscosity to 3000 - 15000 cps

[0101] Comparative Example 1

[0102] This comparative example provides a probiotic shampoo for regulating scalp microecology, whose components are similar to those of Example 1, and the difference is only that: only probiotics and prebiotics are used in the probiotic composition.

[0103] Comparative Example 2

[0104] This comparative example provides a probiotic shampoo for regulating scalp microecology, whose components are similar to those of Example 1, and the difference is only that: only probiotics are used in the probiotic composition.

[0105] Test Example

[0106] Sample completion status:

[0107] Example 1: The planned number of enrolled subjects was 33, and the actual number of enrolled subjects was 32. Among them, 2 subjects dropped out due to personal reasons. Finally, the number of subjects who completed the test was 30, and the number of subjects for statistical analysis was 30.

[0108] Comparative Example 1: The planned number of enrolled subjects was 33, and the actual number of enrolled subjects was 33. Among them, 1 subject dropped out due to personal reasons. Finally, the number of subjects who completed the test was 32, and the number of subjects for statistical analysis was 32.

[0109] Comparative Example 2: The planned number of enrolled subjects was 33, and the actual number of enrolled subjects was 32. Among them, 2 subjects dropped out due to personal reasons. Finally, the number of subjects who completed the test was 30, and the number of subjects for statistical analysis was 30.

[0110] (1) The scalp cutin layer moisture content of the probiotic shampoo prepared in the examples and comparative examples is shown in Table 4:

[0111] Table 4

[0112]

[0113] It can be found from Table 4 that after 14 days and 28 days of use, the scalp cutin layer moisture content of Example 1 was significantly higher than that of Comparative Example 1 and Comparative Example 2, and the scalp cutin layer moisture content of Comparative Example 1 was also significantly higher than that of Comparative Example 2. This shows that the combined use of probiotics + prebiotics + postbiotics has a more significant moisturizing effect on the scalp.

[0114] The test results of the scalp cutin layer moisture content of the examples of the present invention are shown in Table 5:

[0115] Table 5 Comparison of cutin layer moisture content before and after use

[0116]

[0117] The statistical analysis results of the scalp cutin layer moisture content of Example 1 of the present invention are shown in Table 6.

[0118] Table 6 Statistical analysis results of cutin layer moisture content

[0119]

[0120]

[0121] * The Shapiro-Wilk Test method was used to perform a significance test on the normal distribution of the data improvement values.

[0122] The change trend, difference and change rate of the cutin layer moisture content in the test area of the subjects are as Figure 1 , Figure 2 , Figure 3 shown. From Table 5 - Table 6, Figures 1 - 3It can be seen that, compared with before use, the water content of the scalp stratum corneum increased by 15.44% on the 14th day when the subjects used the test product; compared with before use, the water content of the scalp stratum corneum increased significantly by 18.53% (p<0.05) on the 28th day when the subjects used the test product; after 28 days of using the test product, the water content of the scalp stratum corneum increased significantly by 18.53%, indicating that it has a significant moisturizing effect after 28 days.

[0123] (2) Transdermal water loss of the scalp

[0124] The test results of the transdermal water loss of the scalp of the probiotic shampoos prepared in the examples and comparative examples are shown in Table 7.

[0125] Table 7

[0126]

[0127] It can be found from Table 7 that: after 14 days and 28 days of use, the transdermal water loss of the scalp in Example 1 was significantly lower than that in Comparative Example 1 and Comparative Example 2, and the transdermal water loss of the scalp in Comparative Example 1 was also significantly lower than that in Comparative Example 2. It shows that the combined use of probiotics + prebiotics + postbiotics has a more significant effect on repairing the scalp barrier.

[0128] The test results of the transdermal water loss of the scalp in Example 1 of the present invention are shown in Table 8:

[0129] Table 8 Comparison of transdermal water loss before and after use

[0130]

[0131] The statistical analysis results of the transdermal water loss of the scalp in Example 1 of the present invention are shown in Table 9.

[0132] Table 9 Statistical analysis results of transdermal water loss

[0133]

[0134] * The Shapiro-Wilk Test method was used to perform a significance test on the normal distribution of the data improvement values.

[0135] The changing trends, differences and change rates of the transdermal water loss in the test areas of the subjects can be seen in Figure 4 、 Figure 5 、 Figure 6 。

[0136] From Tables 8-9, Figures 4 - 6It can be seen that, compared with before use, the transdermal water loss of the scalp decreased by 4.87% on the 14th day of using the test product; compared with before use, the transdermal water loss of the scalp significantly decreased by 16.37% (p<0.001) on the 28th day of using the test product; after 28 days of using the test product, the transdermal water loss of the scalp significantly decreased by 16.37%, indicating a significant effect of repairing the barrier after 28 days.

[0137] (3) Scalp skin oil content

[0138] The test results of the scalp skin oil content of the examples and comparative examples of the present invention are shown in Table 10.

[0139] Table 10

[0140]

[0141] It can be found from Table 10 that: after 14 days and 28 days of use, the scalp skin oil content of Example 1 was significantly lower than that of Comparative Example 1 and Comparative Example 2, and the scalp skin oil content of Comparative Example 1 was also significantly lower than that of Comparative Example 2. It shows that the combined use of probiotics + prebiotics + postbiotics has a more significant oil control effect on the scalp.

[0142] The test results of the scalp skin oil content of Example 1 of the present invention are shown in Table 11:

[0143] Table 11 Comparison of skin oil content before and after use

[0144]

[0145] The statistical analysis results of the scalp skin oil content of Example 1 of the present invention are shown in Table 12.

[0146] Table 12 Statistical analysis results of skin oil content

[0147]

[0148] * The Shapiro-Wilk Test method was used to conduct a significance test on the normal distribution of the data improvement value.

[0149] The change trend, difference and change rate of the skin oil content in the test area of the subjects can be seen in Figure 7 、 Figure 8 、 Figure 9 。

[0150] From Tables 11-12, Figures 7 - 9It can be seen that, when comparing the scalp skin oil content of the subjects on the 14th day of using the test product with that before use, it was significantly reduced by 23.10% (p<0.01); when comparing the scalp skin oil content of the subjects on the 28th day of using the test product with that before use, it was significantly reduced by 43.17% (p<0.001); after using the test product for 14 days, the scalp skin oil content was significantly reduced by 23.10%, indicating a significant oil control effect after 14 days; after using the test product for 28 days, the scalp skin oil content was significantly reduced by 43.17%, indicating a significant oil control effect after 28 days.

[0151] (4) The number of dandruff

[0152] The test results of the number of dandruff in the examples and comparative examples of the present invention are shown in Table 13.

[0153] Table 13

[0154]

[0155] It can be found from Table 13 that: after using for 14 days and 28 days, the number of dandruff in Example 1 was significantly reduced compared with Comparative Example 1 and Comparative Column 2, and the number of dandruff in Comparative Example 1 was also significantly reduced compared with Comparative Example 2. It shows that the combined use of probiotics + prebiotics + probiotics has a more significant anti-dandruff effect on the scalp.

[0156] The specific test results of the number of dandruff in Example 1 of the present invention are shown in Table 14:

[0157] Table 14 Comparison of the number of dandruff before and after use

[0158]

[0159] The statistical analysis results of the number of dandruff in Example 1 of the present invention are shown in Table 15.

[0160] Table 15 Statistical analysis results of the number of dandruff

[0161]

[0162]

[0163] * The Shapiro-Wilk Test method was used to perform a significance test on the normal distribution of the data improvement value.

[0164] The change trend, difference and change rate of the number of dandruff in the test area of the subjects can be seen in Figure 10 、 Figure 11 、 Figure 12 。

[0165] From Tables 14-15, Figures 10 - 12It can be seen that when comparing the number of dandruff on the 14th day of using the test product with that before use, the number of dandruff decreased significantly by 25.56% (p < 0.05); when comparing the number of dandruff on the 28th day of using the test product with that before use, the number of dandruff decreased significantly by 73.08% (p < 0.001); after using the test product for 14 days, the number of dandruff decreased significantly by 25.56%, indicating a significant anti-dandruff effect after 14 days; after using the test product for 28 days, the number of dandruff decreased significantly by 73.08%, indicating a significant anti-dandruff effect after 28 days.

[0166] (5) Effective cases of anti-dandruff

[0167] The transdermal anti-dandruff effects of 3 subjects (subject numbers 003, 005, and 024) with obvious anti-dandruff effects after using the product for 28 days are shown in Figure 13 、 Figure 14 、 Figure 15 . It can be seen that after the subjects used the test product for 28 days, there was a very significant anti-dandruff effect. Figures 13 - 15

[0168] (6) Results of safety evaluation of human skin irritation test for cosmetics

[0169] Let the subjects directly use the test product according to the usage characteristics and methods indicated on the cosmetics product label. Observe or follow up with the subjects by phone once a week for skin reactions, and record the results according to the grading standard of skin adverse reactions in the current effective technical specification (2015 Edition of Technical Specifications for Cosmetics Safety). The trial period is 4 weeks (28 days).

[0170] Table 16 Results of human skin irritation test for cosmetics

[0171]

[0172] To sum up, the test results were obtained after 30 Chinese healthy subjects aged 25 - 55 years old with neutral or oily scalps, dandruff, and mild scalp itching symptoms used the test product for 28 days. The test results show that the product has the following effects:

[0173] The results of instrument detection show that after using the test product for 14 days, the scalp skin oil content of the subjects decreased significantly by 23.10%, and the number of dandruff decreased significantly by 25.56%. The above results indicate that after using the test product for 14 days, it has a significant oil control effect and a significant anti-dandruff effect.

[0174] ​After 28 days of using the test product, the water content of the stratum corneum of the subjects' scalps increased significantly by 18.53%, the transdermal water loss of the scalps decreased significantly by 16.37%, the sebum content of the scalp skin decreased significantly by 43.17%, and the dandruff count decreased significantly by 73.08%. The above results indicate that after 28 days of using the test product, it has significant moisturizing effect, significant barrier repair effect, significant sebum control effect, and significant anti-dandruff effect.

[0175] The results of the product safety evaluation show that during the test period, none of the subjects had skin adverse reactions such as skin erythema, infiltration, edema, papules, blisters, etc., nor did they have acne, enlarged pores, dull skin color, barrier damage-related symptoms (including but not limited to skin itching, stinging, fever, tightness, redness, increased red blood streaks, etc.) and allergic symptoms.

[0176] Obviously, the above embodiments are merely examples given for clear illustration and are not limitations on the implementation manners. For those of ordinary skill in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to enumerate all the implementation manners here. And the obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A probiotic shampoo for regulating scalp microecology, characterized in that, by weight, it comprises the following components: 12 parts - 42 parts of surfactant, 1 part - 17 parts of hair conditioning ingredient, 0.35 part - 13 parts of probiotic composition, 1 part - 6 parts of stabilizing thickener, 0.06 part - 0.12 part of preservative, 1 part - 6 parts of appearance regulator, 0.05 part - 1 part of pH regulator, 0.5 part - 2 parts of fragrance and 40 parts - 65 parts of water; the probiotic composition includes probiotics, prebiotics and postbiotics.

2. The probiotic shampoo according to claim 1, characterized in that, by weight, the probiotic composition includes 0.11 part - 4 parts of probiotics, 0.11 part - 3 parts of prebiotics and 0.13 part - 6 parts of postbiotics.

3. The probiotic shampoo according to claim 1, characterized in that, the surfactant is one or more of anionic surfactant, cationic surfactant, amphoteric surfactant and nonionic surfactant.

4. The probiotic shampoo according to claim 3, characterized in that, by weight, the surfactant includes 11 parts - 30 parts of anionic surfactant, 0.2 part - 2 parts of cationic surfactant, 0.5 part - 6 parts of amphoteric surfactant and 1 part - 4 parts of nonionic surfactant.

5. The probiotic shampoo according to claim 1, characterized in that, the hair conditioning ingredient is selected from one or more of poly(dimethylsiloxane) alcohol, poly(dimethylsiloxane), hydrogenated starch hydrolysate, panthenol, panthenol ethyl ether and hydrolyzed vegetable protein.

6. The probiotic shampoo according to claim 1, characterized in that, the stabilizing thickener is selected from acrylic acid (ester) copolymer and / or sodium chloride.

7. The probiotic shampoo according to claim 1, characterized in that, the preservative is selected from one or more of Kathon, hexamidine di(2-hydroxyethylsulfonate), sodium benzoate and phenoxyethanol.

8. The probiotic shampoo according to claim 1, characterized in that, the appearance regulator is ethylene glycol distearate and / or pigment.

9. The probiotic shampoo according to claim 1, characterized in that, the pH regulator is selected from one or more of citric acid, lactic acid, sodium hydroxide, triethanolamine and arginine; the fragrance is selected from essence or natural essential oil.

10. The preparation method of the probiotic shampoo according to any one of claims 1 - 9, characterized in that, comprises the following steps: S1. Add the cationic surfactant into water and stir to dissolve evenly, then add the anionic surfactant, heat to 80°C - 85°C to dissolve, and then add the amphoteric surfactant, nonionic surfactant and appearance regulator, stir to dissolve evenly, and keep stirring for 20 min - 30 min; S2. Cool the solution obtained in step S1 to 60°C - 65°C, stir to dissolve, and add the stabilizing thickener; cool to 50°C - 55°C, and stir to cool until obvious pearlescence appears; S3. After the pearlescent agent is completely added, add hair conditioning ingredients and stir evenly; then continue to add fragrance, preservative, probiotic composition and appearance regulator, and stir evenly; S4. Use a pH regulator to adjust the pH value of the solution prepared in step S3 to 5.0 - 6.5, and adjust the viscosity to 3000 cps - 15000 cps to prepare the probiotic shampoo with the function of regulating scalp microecology.

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