Foam type composite disinfectant as well as preparation method and application thereof

Abstract

The invention provides a foam type composite disinfectant as well as a preparation method and application thereof, and relates to the technical field of disinfectants. The foam type composite disinfectant comprises ethanol, benzalkonium chloride, chlorhexidine acetate, a composite surfactant, a chelating agent, a humectant, a thickening agent, a foam stabilizer and deionized water. The composite surface active agent is cocamidopropyl betaine and alkyl glycoside; the molar ratio of the cocamidopropyl betaine to the alkyl glycoside is 1: (1-3); the pH (Potential of Hydrogen) of the foam type composite disinfectant is 5.5 to 6.5. The foam type composite disinfectant overcomes the problems of single component, high concentration, strong irritation, poor foam stability, poor component compatibility and the like of the existing disinfectant, has the advantages of efficient sterilization, low irritation, lasting foam, long-term stability and the like, and has a good application prospect.

Description

Technical Field

[0001] The present invention relates to the technical field of disinfectant solutions, and particularly to a foam-type composite disinfectant solution, a preparation method thereof, and an application thereof. Background Art

[0002] A disinfectant is a preparation that kills or inhibits pathogenic microorganisms by chemical or biological methods, and is widely used in fields such as medical treatment, household, industry, agriculture, and public facilities. Its core function is to cut off the transmission route of pathogens and ensure health and safety. Traditional disinfectant products generally have the following limitations. First, traditional disinfectant products generally have a single component and a high concentration of active ingredients, with strong irritation, which easily causes skin dryness and a burning sensation.

[0003] A foam-type disinfectant is a disinfection product used in the form of foam, which has unique product characteristics, a wide range of application scenarios, and a certain market trend. Foam-type disinfectants have advantages such as strong adhesion, integration of cleaning and disinfection, diverse components, environmental protection and safety. However, foam-type disinfectants have problems such as the inability to combine foam stability and disinfection efficacy, poor foaming performance and foam stability, poor compatibility between multiple components, and poor long-term storage stability.

[0004] Therefore, it is necessary to develop a foam-type composite disinfectant solution with advantages such as high-efficiency sterilization, low irritation, long-lasting foam, and long-term stability to overcome these problems. Summary of the Invention

[0005] In view of this, the present invention provides a foam-type composite disinfectant solution, a preparation method thereof, and an application thereof. The foam-type composite disinfectant solution of the present invention overcomes the problems of traditional disinfectants such as single component, high concentration, strong irritation, poor foam stability, and poor component compatibility, and has advantages such as high-efficiency sterilization, low irritation, long-lasting foam, and long-term stability, and has good application prospects.

[0006] The first aspect of the present invention is to provide a foam-type composite disinfectant solution, comprising the following raw materials:

[0007] ethanol 15 wt.% - 20 wt.%, benzalkonium chloride 0.05 wt.% - 0.3 wt.%, chlorhexidine acetate 0.2 wt.% - 1.5 wt.%, composite surfactant 2 wt.% - 6 wt.%, chelating agent 0.1 wt.% - 0.5 wt.%, humectant 4 wt.% - 5 wt.%, thickener 0.05 wt.% - 1 wt.%, foam stabilizer 0.1 wt.% - 0.8 wt.%, and the balance is deionized water.

[0008] Preferably, the composite surfactant is cocamidopropyl betaine (CAPB) and alkyl polyglycoside (APG), the molar ratio of cocamidopropyl betaine to alkyl polyglycoside is 1:(1 - 3), and the carbon chain length of the alkyl polyglycoside is C12 -C 14 The chelating agent is EDTA-2Na, the humectant is glycerol and / or hyaluronic acid, the thickener is at least one of xanthan gum, guar gum, carboxymethyl cellulose, hydroxypropyl cellulose, and polyacrylamide, the foam stabilizer is at least one of polydimethylsiloxane copolyol, hydroxyethyl cellulose, and sodium alginate, and the pH of the foam-type composite disinfectant is 5.5-6.5.

[0009] Preferably, when the thickener is at least one of xanthan gum, guar gum, and polyacrylamide, the preferred thickener is xanthan gum, and the dosage of the thickener is 0.1wt.% - 0.5wt.%. When the thickener is carboxymethyl cellulose and / or hydroxypropyl cellulose, the dosage of the thickener is 0.1wt.% - 1wt.%. When the foam stabilizer is polydimethylsiloxane copolyol, the dosage of the foam stabilizer is 0.05wt.% - 0.1wt.%. When the foam stabilizer is hydroxyethyl cellulose and / or sodium alginate, the dosage of the foam stabilizer is 0.1wt.% - 0.5wt.%.

[0010] The second aspect of the present invention is to provide a preparation method of a foam-type composite disinfectant, including the following steps:

[0011] Pre-disperse the thickener; mix part of deionized water with the chelating agent and the humectant, and then add the pre-dispersed thickener to obtain mixture A; mix ethanol with benzalkonium chloride and chlorhexidine acetate to obtain mixture B; slowly add mixture B to mixture A, homogenize, then add the foam stabilizer, continue to homogenize, add deionized water to make up, adjust the pH of the system, and sieve to obtain the foam-type composite disinfectant.

[0012] Preferably, the homogenization speed is 2000-3000rpm, the homogenization time is 3-5min, and the continuous homogenization time is 5-10min.

[0013] The third aspect of the present invention is to provide an application of the foam-type composite disinfectant in medical, public health, and daily life disinfection.

[0014] Compared with the prior art, the beneficial technical effects of the present invention are:

[0015] Through the ternary compounding system of ethanol, BZK, and chlorhexidine acetate, the present invention realizes multi-target synergistic sterilization, and can maintain high-efficiency broad-spectrum sterilization while reducing the concentration of a single component.

[0016] The present invention optimizes the foam performance and the stability of the disinfectant through a composite surfactant, and ensures the stability and safety of the system by regulating the pH of the system.

[0017] Through the step-by-step mixing and homogenization process, the present invention avoids the caking of thickeners and the volatilization of ethanol, forms submicron emulsions, ensures smooth foam pumping and non-collapse, prevents the degradation of active ingredients, and guarantees the bactericidal activity.

[0018] The foam of the disinfectant of the present invention has strong adhesion, is suitable for vertical surfaces, can reduce the risk of liquid penetration, and can relieve skin dryness caused by frequent use, improving comfort. Detailed implementation manners

[0019] Next, in combination with the embodiments of the present invention, the technical solutions of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0020] The first aspect of the present invention is to provide a foam-type composite disinfectant, comprising the following raw materials:

[0021] Ethanol 15wt.% - 20wt.%, benzalkonium chloride 0.05wt.% - 0.3wt.%, chlorhexidine acetate 0.2wt.% - 1.5wt.%, composite surfactant 2wt.% - 6wt.%, chelating agent 0.1wt.% - 0.5wt.%, humectant 4wt.% - 5wt.%, thickener 0.05wt.% - 1wt.%, foam stabilizer 0.1wt.% - 0.8wt.%, and the balance is deionized water.

[0022] There is a synergistic effect among ethanol, benzalkonium chloride, and chlorhexidine acetate in the foam-type composite disinfectant of the present invention. Among them, benzalkonium chloride and chlorhexidine acetate are the main ones, and ethanol mainly plays a synergistic role.

[0023] Benzalkonium chloride is a cationic surfactant belonging to the quaternary ammonium group. It adsorbs on the surface of microorganisms through cationic groups and destroys the cell membrane permeability. It mainly has three uses: as a biocide, a cationic surfactant, and a phase transfer agent in the chemical industry. Benzalkonium chloride is a heterogeneous mixture of various even-alkyl-chain alkyl benzyl chlorides. The advantage of benzalkonium chloride disinfectant different from ethanol- or hydrogen peroxide-based disinfectants is that it does not cause skin burning when applied to injured skin.

[0024] Chlorhexidine acetate has a broad antibacterial spectrum and strong antibacterial activity, and its mechanism of action is to change the permeability of the bacterial cell membrane.

[0025] The present invention utilizes a ternary compound disinfection system to act through a multi-target mechanism, reducing the irritation of the disinfectant to the skin while ensuring the disinfection efficacy.

[0026] The composite surfactant in the present invention is CAPB and APG, the molar ratio of CAPB and APG is 1:(1 - 3), and the carbon chain length of the alkyl polyglycoside is C 12 -C 14 .

[0027] In the present invention, CAPB provides instantaneous foaming power, rapidly reduces the surface tension through intermolecular charge interactions, and forms delicate foam. Experimental data during the R & D process of the present invention shows that when CAPB is used alone, the initial foaming height is relatively high, but the foam stability is poor, manifested as a short half-life. While APG enhances the foam stability through the close arrangement of its hydrophobic chains, and the long carbon chains of APG form a dense film layer at the gas-liquid interface, extending the foam half-life. At the same time, its low critical micelle concentration can reduce the migration of surfactant molecules. In addition, the weak cationic property of CAPB inhibits the precipitation reaction of components, and the hydroxyl structure of APG forms a hydrogen bond network with ethanol, preventing the stratification of the system caused by ethanol volatilization. That is, the use of the composite surfactant improves the stability of the disinfectant.

[0028] In addition, the present invention strictly limits the ratio of CAPB and APG. Under this ratio, the foam performance of the disinfectant, the stability of the disinfectant, and the compatibility between components are all within a reasonable range. Beyond this range, the above performances all show varying degrees of decline. The main reason for the above problems may be that under this ratio, CAPB and APG are charge complementary, which can avoid charge conflicts with the cationic disinfectant in the formula and prevent precipitation. And the polar head of CAPB and the glycosyl group of APG form mixed micelles, reducing the loss of ethanol volatilization. The hydroxyl group of APG and the betaine group of CAPB form a hydrogen bond network, enhancing the compatibility between raw materials. At the same time, as can be seen from the above, CAPB provides instantaneous high foaming power, and APG improves foam stability, but an excessive amount of APG will lead to too high foam viscosity, affecting the pumping performance, and the foaming amount and foam density will be significantly reduced. Therefore, it is necessary to strictly control the ratio of CAPB and APG.

[0029] The present invention limits the carbon chain length of the APG to C 12 -C 14 . Alkyl polyglycoside refers to alkyl polyglycoside synthesized from glucose and fatty alcohol, abbreviated as APG. Generally, the degree of polymerization n of alkyl polyglycoside is in the range of 1.1 - 3, and R is an alkyl group of C 8 -C 16 . Generally speaking, APG with a carbon chain length of C 8 -C 10 is short-chain APG, and the carbon chain length is C 12 -C14 The APG with medium and long carbon chains. In the disinfectant of the present invention, short-chain APG can provide a faster foaming speed, but the comprehensive foam performance is significantly lower than that of medium and long-chain APG, significantly reducing the comprehensive quality of the disinfectant. Therefore, the present invention limits the carbon chain length of APG to C 12 -C 14 . However, medium and long-chain APG has poor solubility in the system and may undergo hydrophobic-hydrophobic aggregation with cationic groups. Therefore, the present invention overcomes this problem by adopting a higher-intensity homogenization during the preparation process and increasing the dosage of chelating agent.

[0030] The chelating agent in the present invention is EDTA-2Na, the humectant is glycerol and / or hyaluronic acid, the thickener is at least one of xanthan gum, guar gum, carboxymethyl cellulose, hydroxypropyl cellulose, and polyacrylamide, the foam stabilizer is at least one of polydimethylsiloxane copolyol, hydroxyethyl cellulose, and sodium alginate, and the pH of the foam-type composite disinfectant is 5.5 - 6.5.

[0031] In some specific embodiments of the present invention, when the thickener is at least one of xanthan gum, guar gum, and polyacrylamide, the preferred thickener is xanthan gum, and the dosage of the thickener is 0.1 wt.% - 0.5 wt.%. When the thickener is carboxymethyl cellulose and / or hydroxypropyl cellulose, the dosage of the thickener is 0.1 wt.% - 1 wt.%. When the foam stabilizer is polydimethylsiloxane copolyol (Dow 193), the dosage of the foam stabilizer is 0.05 wt.% - 0.1 wt.%. When the foam stabilizer is hydroxyethyl cellulose and / or sodium alginate, the dosage of the foam stabilizer is 0.1 wt.% - 0.5 wt.%.

[0032] The present invention also needs to strictly limit the pH of the disinfectant, which has a crucial impact on the comprehensive performance of the disinfectant.

[0033] First of all, BZK is most stable under neutral to weakly acidic conditions. Alkaline conditions will accelerate the hydrolysis of BZK, generating benzyl alcohol and long-chain tertiary amines, resulting in a decrease in bactericidal activity. CHG has a higher antibacterial activity under weakly acidic conditions, while under alkaline conditions at 7, it is easy to combine with Ca in hard water 2+ / Mg 2+Form insoluble salts, reducing the effectiveness of the disinfectant. The glycosidic bond of APG is prone to hydrolysis and breakage in acidic or strongly alkaline environments, destroying its foam-stabilizing performance. The thickener xanthan gum has a relatively stable colloidal network in weakly acidic conditions. Too low or too high pH will cause the unwinding of its helical structure and a significant decrease in viscosity. In the disinfectant system of the present invention, the charge synergy of BZK and CHG is within the range of pH 5.5 - 6.5. The cationic group of BZK and the protonated biguanide group of CHG are co-adsorbed on the surface of microorganisms, achieving multi-target sterilization by destroying the cell membrane permeability. The slightly acidic environment can enhance the denaturation effect of ethanol on bacterial proteins and improve the disinfection efficiency. When the pH is too high, the concentration of OH - The ion concentration increases, which will reduce the ability of BZK and CHG to adsorb on the surface of microorganisms, and will also affect the critical micelle concentration and foam strength of the surfactant. When the pH is in the range of 5.5 - 6.5, the mixed micelle structure of CAPB and APG in the disinfectant system of the present invention is the most stable, ensuring fine foam and a relatively long half-life. And at this pH, the non-ionic property of APG can buffer the charge repulsion of the cationic surfactant, preventing the aggregation of hydrophobic chains and causing phase separation. EDTA has a strong chelating ability for most metal ions in the range from neutral to weakly alkaline, preventing the destruction of APG micelles due to electrolyte aggregation. Finally, when the pH is in the range of 5.5 - 6.5, it is close to the pH of healthy skin surface (4.5 - 6.5), which can reduce the damage to the skin barrier, avoid dryness or stinging sensation after use, and reduce irritation.

[0034] Therefore, the present invention needs to strictly limit the pH of the disinfectant to achieve the balance among component stability, bactericidal efficacy, system compatibility, and use safety.

[0035] The second aspect of the present invention is to provide a preparation method of a foam-type composite disinfectant solution, including the following steps:

[0036] S1. Accurately weigh each component according to the ratio, and pre-disperse and swell the thickener with a small amount of cold water to avoid caking;

[0037] S2. Add 50% of the formulated amount of deionized water to the reaction kettle, heat it to 40°C - 50°C, successively add the chelating agent and the humectant, stir until completely dissolved, add the pre-dispersed thickener, keep stirring until a uniform colloid is formed, add the composite surfactant, and stir until transparent to obtain mixture A;

[0038] S3. Add ethanol to another container, and successively dissolve benzalkonium chloride and chlorhexidine acetate under the condition of controlling the temperature ≤ 30°C, and stir until clear to obtain mixture B;

[0039] S4. Slowly add mixture B to mixture A, and shear and emulsify it with a homogenizer at a speed of 2000 - 3000 rpm while adding. Continue for 3 - 5 min, then add a foam stabilizer, and continue homogenizing for 5 - 10 min. Make up deionized water to the total formulation amount, mix evenly, adjust the pH of the system to 5.5 - 6.5 with a citric acid - sodium citrate buffer solution, let it stand to defoam for 8 - 24 h, and then sieve to obtain a foam - type composite disinfectant solution.

[0040] In some specific embodiments of the present invention, after sieving the disinfectant solution, there is also a filling step, that is, filling the foam - type composite disinfectant solution into a foam pump - type container to obtain a directly usable foam - type disinfection product.

[0041] In some specific embodiments of the present invention, the sieve mesh size is 200 meshes.

[0042] The present invention forms a uniform colloidal precursor through a raw material pretreatment process, reducing the subsequent stirring time and avoiding problems such as excessive local concentration, viscosity mutation, uneven system viscosity, particle feeling during foam extrusion, or pump head blockage caused by the agglomeration of thickeners. The preparation process of mixture A can provide a basic solvent, promote the dissolution of chelating agents and humectants, and prevent the aggregation of APG micelles. The raw material addition sequence of chelating agent → humectant → thickener → composite surfactant can ensure complete chelation and highly dispersed surfactants, avoiding the destabilization of subsequent surfactants (APG / CAPB) due to electrolytes and improving the mixing efficiency. During the preparation process of mixture B, controlling the temperature ≤ 30 °C can prevent the volatilization loss of ethanol and ensure the complete dissolution of BZK and CHG. The raw material addition sequence of ethanol → BZK → CHG can avoid problems such as the precipitation of BZK and incomplete dissolution of CHG to ensure the stability and bactericidal efficacy of the product. The mixing and homogenization of mixture A and mixture B can use high shear force to break the interfacial tension between the two phases, uniformly disperse mixture A in mixture B to form a submicron emulsion, reduce the curling of the hydrophobic chain of APG caused by excessive local ethanol concentration, and prevent phase separation. Continuing homogenization after adding the foam stabilizer is beneficial to fully disperse the foam stabilizer and improve the foam quality. In addition, the present invention needs to limit the process parameters of shear emulsification. During the preparation process of the foam-type composite disinfectant of the present invention, a rotation speed of 2000 - 3000 rpm can provide sufficient shear force, and 3 - 5 min of main homogenization ensures uniform droplet distribution, disperses air into micron-sized bubbles, avoids the formation of large bubbles, ensures that the pumped foam is delicate and dense, enables the surfactant to form a dense arrangement at the interface, and reduces the coalescence and rupture of bubbles. The homogenization speed and time control the mixing degree of the surfactant and the cationic disinfectant, avoiding precipitation caused by local charge overload. The ethanol phase is quickly dispersed into the aqueous colloidal phase, reducing direct contact with cationic components and lowering the complexation risk. However, too long homogenization time or too high rotation speed will cause the system to heat up or components to degrade, resulting in the viscosity of the disinfectant exceeding the range of 500 - 1500 mPa·s, leading to problems such as difficult pumping (too thick) or easy collapse of foam (too thin). Generally speaking, the present invention needs to control the homogenization parameters to balance the physical dispersion of raw materials, the chemical stability of the disinfectant, and rheological property control to ensure that the foam-type disinfectant has delicate and long-lasting foam performance and long-term stability while having high bactericidal efficiency, and also avoids differences between production batches.

[0043] The third aspect of the present invention is to provide an application of the foam-type composite disinfectant in medical, public health, and daily life disinfection.

[0044] The applications of the disinfectant of the present invention include but are not limited to the following aspects:

[0045] In the medical field, the disinfectant solution of the present invention can be used for disinfecting the skin around surgical incisions, injection sites, or wounds before debridement, and can be used for surface disinfection of non-invasive instruments (such as stethoscopes, sphygmomanometer cuffs). The foam form can reduce the risk of liquid penetration. At the same time, the formula of the disinfectant solution of the present invention can relieve skin dryness caused by frequent use and reduce irritation.

[0046] In the fields of public health and daily life, the strong adhesion of the foam of the disinfectant solution of the present invention can be used to treat vertical surfaces (such as elevator buttons, door handles), extend the contact time of the disinfectant, can be used on the skin surface, and the stable foam performance can enhance the local retention effect. It can be used for disinfection work related to pets to inactivate enveloped viruses such as canine distemper virus and feline calicivirus.

[0047] In addition, in some special fields, the foam performance of the disinfectant solution of the present invention can also achieve good use effects. For example, foam coverage can reduce the risk of aerosol diffusion of viruses and can be used for the inhibition of coronaviruses (enveloped viruses such as influenza virus).

[0048] Generally speaking, the present invention realizes the balance between foam form and bactericidal efficacy through a colloidal stabilization system, and can take into account the needs of public health and household use.

[0049] To further illustrate the present invention, the following will be described in detail through the following examples. The raw materials used in the following examples of the present invention are all commercially available.

[0050] Unless otherwise specified, all tests were repeated 3 times. ANOVA (Analysis of Variance) and Duncan multiple comparison analysis were performed using SPSS 21.0, and the results were expressed as mean ± standard deviation. A significant difference was indicated when P < 0.05.

[0051] Example 1 A foam-type composite disinfectant solution is composed of the following raw materials:

[0052] Ethanol 18 wt.%, Benzalkonium Chloride 0.15 wt.%, Chlorhexidine Acetate 0.8 wt.%, Composite Surfactant 4 wt.%, EDTA-2Na 0.25 wt.%, Glycerol 4.5 wt.%, Xanthan Gum 0.3 wt.%, Polydimethylsiloxane Copolyol 0.45 wt.%, and the balance is deionized water;

[0053] The composite surfactant is composed of CAPB and APG-1214 in a molar ratio of 1:2.

[0054] The preparation method of the foam-type composite disinfectant solution is as follows:

[0055] S1. Accurately weigh each component according to the ratio, and disperse and swell the thickener with a small amount of cold water in advance to avoid caking;

[0056] S2. Add 50% of the formulated amount of deionized water into the reaction kettle, heat it to 40 °C, successively add the chelating agent and the humectant, stir until completely dissolved, add the pre-dispersed thickener, keep stirring until a homogeneous colloid is formed, add the compound surfactant, and stir until transparent to obtain mixture A;

[0057] S3. Add ethanol into another container, and successively dissolve benzalkonium chloride and chlorhexidine acetate at 25 °C, stir until clear to obtain mixture B;

[0058] S4. Slowly add mixture B into mixture A, while adding, shear and emulsify with a homogenizer at a speed of 2500 rpm for 4 min, then add the foam stabilizer, continue homogenizing for 8 min, make up deionized water to the total formulated amount, mix evenly, adjust the pH of the system to 6.0 with citric acid-sodium citrate buffer solution, stand for defoaming for 24 h, and sieve to obtain the foam-type compound disinfectant.

[0059] Example 2 A foam-type compound disinfectant is composed of the following raw materials:

[0060] Ethanol 15 wt.%, benzalkonium chloride 0.05 wt.%, chlorhexidine acetate 0.2 wt.%, compound surfactant 2 wt.%, EDTA-2Na 0.1 wt.%, glycerol 4 wt.%, xanthan gum 0.1 wt.%, polydimethylsiloxane copolymer alcohol 0.1 wt.%, and the balance is deionized water;

[0061] The compound surfactant is composed of CAPB and APG-1214 in a molar ratio of 1:3.

[0062] The preparation method of the foam-type compound disinfectant is as follows:

[0063] S1. Accurately weigh each component according to the ratio, and pre-disperse and swell the thickener with a small amount of cold water to avoid caking;

[0064] S2. Add 50% of the formulated amount of deionized water into the reaction kettle, heat it to 40 °C, successively add the chelating agent and the humectant, stir until completely dissolved, add the pre-dispersed thickener, keep stirring until a homogeneous colloid is formed, add the compound surfactant, and stir until transparent to obtain mixture A;

[0065] S3. Add ethanol into another container, and successively dissolve benzalkonium chloride and chlorhexidine acetate at 25 °C, stir until clear to obtain mixture B;

[0066] S4. Slowly add mixture B to mixture A, while adding, shear and emulsify with a homogenizer at a speed of 2000 rpm for 4 min. Then add a foam stabilizer and continue homogenizing for 8 min. Make up deionized water to the total formulation amount, mix evenly, adjust the pH of the system to 6.0 with a citric acid-sodium citrate buffer solution, let it stand to defoam for 24 h, and sieve to obtain a foam-type composite disinfectant solution.

[0067] Example 3 A foam-type composite disinfectant solution is composed of the following raw materials:

[0068] Ethanol 20 wt.%, benzalkonium chloride 0.3 wt.%, chlorhexidine acetate 1.5 wt.%, composite surfactant 6 wt.%, EDTA-2Na 0.5 wt.%, hyaluronic acid 5 wt.%, xanthan gum 0.5 wt.%, polydimethylsiloxane copolyol 0.8 wt.%, and the balance is deionized water;

[0069] The composite surfactant is composed of CAPB and APG-1214 in a molar ratio of 1:1.

[0070] The preparation method of the foam-type composite disinfectant solution is as follows:

[0071] S1. Weigh each component accurately according to the ratio. Pre-disperse and swell the thickener with a small amount of cold water to avoid caking.

[0072] S2. Add 50% of the formulation amount of deionized water to the reaction kettle, heat to 40 °C, add the chelating agent and the moisturizing agent in sequence, stir until completely dissolved, add the pre-dispersed thickener, keep stirring until a uniform colloid is formed, add the composite surfactant, and stir until transparent to obtain mixture A.

[0073] S3. Add ethanol to another container, dissolve benzalkonium chloride and chlorhexidine acetate in sequence under the condition of 25 °C, and stir until clear to obtain mixture B.

[0074] S4. Slowly add mixture B to mixture A, while adding, shear and emulsify with a homogenizer at a speed of 3000 rpm for 4 min. Then add a foam stabilizer and continue homogenizing for 8 min. Make up deionized water to the total formulation amount, mix evenly, adjust the pH of the system to 6.0 with a citric acid-sodium citrate buffer solution, let it stand to defoam for 24 h, and sieve to obtain a foam-type composite disinfectant solution.

[0075] Comparative Example 1

[0076] Same as Example 1, the difference is that: the composite surfactant is replaced with an equal amount of CAPB.

[0077] Comparative Example 2

[0078] Same as Example 1, with the difference that: the composite surfactant is replaced with an equal amount of APG-1214.

[0079] Comparative Example 3

[0080] Same as Example 1, with the difference that: the composite surfactant is composed of CAPB and APG-1214 in a molar ratio of 1:0.5.

[0081] Comparative Example 4

[0082] Same as Example 1, with the difference that: the composite surfactant is composed of CAPB and APG-1214 in a molar ratio of 1:4.

[0083] Comparative Example 5

[0084] Same as Example 1, with the difference that: APG-1214 is replaced with an equal amount of APG-0810.

[0085] Comparative Example 6

[0086] Same as Example 1, with the difference that:

[0087] (1) The homogenizer shears and emulsifies at a speed of 1000 rpm;

[0088] (2) The homogenizer shears and emulsifies at a speed of 4000 rpm.

[0089] Comparative Example 7

[0090] Same as Example 1, with the difference that:

[0091] (1) Use citric acid-sodium citrate buffer to adjust the pH of the system to 5.0;

[0092] (2) Use phosphate buffer to adjust the pH of the system to 7.0.

[0093] Test Example 1

[0094] Place the foam-type composite disinfectants provided in Examples 1-3 and Comparative Examples 1-6 in an oven at 54 °C for 14 days, and test the decline rate of the bactericidal active ingredient in the disinfectant. The results are shown in Table 1.

[0095] Table 1 Decline rate of the bactericidal active ingredient in the disinfectant

[0096]

[0097]

[0098] Note: Different lowercase letters in the same column in the table indicate significant differences between the two, P < 0.05.

[0099] Test Example 2

[0100] According to the "Disinfection Technical Specification", the killing logarithm values of the foam-type composite disinfectants provided in Examples 1-3 and Comparative Examples 1-6 were measured, and the results are shown in Table 2.

[0101] Table 2 Killing logarithm values

[0102]

[0103] Note: * indicates P < 0.05 compared with Example 1, and ** indicates P < 0.01 compared with Example 1.

[0104] CAPB and APG cooperate to destroy the biofilm structure. When using CAPB alone, the enhancing effect of disinfectant penetration is limited. When using APG alone, only part of the biofilm can be destroyed, and chlorhexidine cannot effectively penetrate into the deep flora. Insufficient proportion of APG leads to insufficient biofilm destruction ability, while excessive amount leads to micelles wrapping chlorhexidine, resulting in a decrease in the concentration of active ingredients in the biofilm. Shortening the carbon chain of APG has little effect on the disinfectant effect, but has a greater impact on the foaming performance. The homogenization speed is slow, the emulsification is insufficient, and excessive shearing leads to the decomposition of active ingredients, and the bactericidal effects are all affected to varying degrees. Acidic conditions weaken the chelating ability of EDTA-2Na, the biofilm integrity is not destroyed, and the penetration of chlorhexidine is limited. Alkaline environment accelerates the hydrolysis of chlorhexidine, and the effect of EDTA-2Na is limited and the biofilm is not removed.

[0105] Test Example 3

[0106] The foam-type composite disinfectants provided in Examples 1-3 and Comparative Examples 1-6 were pumped onto the metal material surface through a foam pump pressure container, and the foam disappearance time (the time when more than 50% of the foam disappears was used as the disappearance time) and the foaming quality were observed, and the results are shown in Table 3.

[0107] Table 3 Foaming performance

[0108]

[0109] Note: Different lowercase letters in the same column in the table indicate significant differences between the two, P < 0.05, and the foaming amount in the table is an estimate.

[0110] The above-described embodiments only represent several implementation manners of the present invention. The description is relatively specific and detailed, but it should not be construed as a limitation on the scope of the invention patent. It should be noted that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can still be made, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the present invention patent shall be subject to the appended claims.

Claims

1. A foam type composite disinfectant, characterized in that: Including the following ingredients: Ethanol 15wt.%-20wt.%, benzalkonium chloride 0.05wt.%-0.3wt.%, chlorhexidine acetate 0.2wt.%-1.5wt.%, complex surfactant 2wt.%-6wt.%, chelating agent 0.1wt.%-0.5wt.%, humectant 4wt.%-5wt.%, thickener 0.05wt.%-1wt.%, foam stabilizer 0.1wt.%-0.8wt.%, and the balance is deionized water; The composite surfactant is cocamidopropyl betaine and alkyl glucoside; The molar ratio of cocamidopropyl betaine to alkyl glucoside is 1:(1-3); The pH value of the foam type composite disinfectant is 5.5-6.

5.

2. The foam type composite disinfectant according to claim 1, characterized in that: The chelating agent is EDTA-2Na.

3. The foam type composite disinfectant according to claim 1, characterized in that: The moisturizing agent is glycerin and / or hyaluronic acid.

4. The foam type composite disinfectant according to claim 1, characterized in that: The thickener is at least one of xanthan gum, guar gum, carboxymethyl cellulose, hydroxypropyl cellulose and polyacrylamide.

5. The foam type composite disinfectant according to claim 1, characterized in that: The foam stabilizer is at least one of dimethicone copolyol, hydroxyethyl cellulose and sodium alginate.

6. The method for preparing the foam type composite disinfectant according to any one of claims 1 to 5, characterized in that: The following steps are involved: The thickener is pre-dispersed; a portion of deionized water is mixed with a chelating agent and a moisturizing agent, and then the pre-dispersed thickener is added to obtain a mixture A; ethanol is mixed with benzalkonium chloride and chlorhexidine acetate to obtain a mixture B; the mixture B is slowly added to the mixture A, homogenized, and then a foam stabilizer is added, the homogenization is continued, deionized water is added, the pH value of the system is adjusted, and sieving is performed to obtain a foam type composite disinfectant.

7. The preparation method according to claim 6, characterized in that: The homogenizing speed is 2000-3000 rpm.

8. The preparation method according to claim 6, characterized in that: The standing defoaming time is 8-24h.

9. The preparation method according to claim 6, characterized in that: After the disinfectant is screened, a filling step is also included.

10. Application of a foam type composite disinfectant in medical treatment, public health and daily life disinfection, characterized in that: The foam type composite disinfectant is the foam type composite disinfectant described in any one of claims 1 to 5 or the foam type composite disinfectant prepared by the method described in any one of claims 1 to 9.

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