Traditional Chinese veterinary medicine formula for preventing and controlling distemper swine disease and preparation process of traditional Chinese veterinary medicine formula

Abstract

The invention relates to the technical field of veterinary traditional Chinese medicines, and provides a traditional Chinese veterinary medicine formula for preventing and controlling a distemper swine disease and a preparation process of the traditional Chinese veterinary medicine formula. The traditional Chinese veterinary medicine formula for preventing and controlling the distemper swine disease is prepared from the following raw materials in parts by weight: 30 to 50 parts of honeysuckle flower extract, 30 to 50 parts of wild chrysanthemum flower extract, 20 to 30 parts of baicalin, 30 to 50 parts of radix isatidis, 20 to 30 parts of radix salviae miltiorrhizae, 20 to 30 parts of radix curcumae, 30 to 50 parts of fructus forsythiae, 20 to 30 parts of fructus gardeniae, 20 to 30 parts of nacre mother of pearl, 5 to 10 parts of realgar, 20 to 30 parts of cornu bubali extract, 30 to 50 parts of poria cocos, 20 to 30 parts of rhizoma atractylodis macrocephalae, 30 to 50 parts of rhizoma dioscoreae, 30 to 50 parts of coix seed, 10 to 20 parts of fructus amomi, 20 to 30 parts of radix platycodonis and 10 to 20 parts of liquorice root. According to the technical scheme, the problems of slow effect and low bioavailability of the traditional Chinese veterinary medicine preparation process in the related technology are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of veterinary traditional Chinese medicine. Specifically, it relates to a traditional Chinese veterinary medicine formula for preventing and controlling febrile swine diseases and its preparation process. Background Art

[0002] In recent years, febrile swine diseases have dealt a heavy blow to the global pig farming industry. Their rapid transmission speed and high fatality rate have led to a large number of live pigs dying, causing incalculable economic losses to farmers and related enterprises. As a major producer and consumer of pork, China has been particularly significantly impacted by the invasion of febrile swine diseases, seriously threatening the country's live pig supply and market stability. The pig farming industry, as an important pillar industry of agriculture, has been severely affected. A large number of live pigs have been infected and died, frequent epidemics have occurred in pig farms, the pig mortality rate has remained high, and many farmers have faced huge economic losses. Some small-scale farms have even closed down as a result.

[0003] The transmission routes of febrile swine disease viruses are extensive. They can be directly transmitted to infect pigs or indirectly transmitted through contaminated items, feed, vehicles, etc., and can also be transmitted through vector organisms such as ticks, which makes prevention and control work extremely difficult. The virus severely damages the pig body, causing multiple organ failure in pigs and triggering a series of serious symptoms such as high fever, skin cyanosis, and bleeding. Currently, there is no effective vaccine to prevent it.

[0004] In the field of animal disease prevention and control, many scholars and research institutions at home and abroad have carried out extensive research on diseases such as febrile swine diseases. Certain progress has been made abroad in vaccine research and development and biosafety prevention and control technologies. For example, in vaccine research and development, through cell culture technology and bio-fermentation engineering technology, in-depth exploration has been carried out on aspects such as virus culture, amplification, and antigen expression. In cell culture technology, cell density and virus amplification efficiency have become key factors determining the output of animal vaccines. Researchers continuously optimize the vaccine production process by regulating the nutrient supply of the cell culture environment and the operating parameters of bioreactors to improve the yield and quality of vaccines.

[0005] Domestic research on animal disease prevention and control is also continuously deepening. On the one hand, in the field of traditional Chinese medicine prevention and treatment, numerous studies have shown that traditional Chinese medicine has unique advantages in improving animal immunity and antiviral properties. Some studies focus on the extraction process of traditional Chinese medicine, exploring the influence of different extraction methods on the extraction rate of effective components of traditional Chinese medicine to improve the efficacy of traditional Chinese medicine preparations. On the other hand, for the prevention and control of febrile swine diseases, domestic efforts are also being made to actively develop effective prevention and control products and technologies. Although no breakthrough progress has been made in vaccine research and development, continuous improvements have been made in prevention and control technologies and management measures, such as strengthening the biosafety management of farms, epidemic monitoring, and early warning.

[0006] However, there are still many deficiencies in the current preparation process of products for preventing and controlling febrile swine diseases. The research and development of existing vaccines face many challenges, such as the difficulty of virus cultivation and the lack of precision in antigen detection methods, resulting in the production efficiency and quality of vaccines being difficult to meet the actual needs. In the aspect of traditional Chinese medicine (TCM) prevention and control products, although TCM has the advantages of being green and safe, the traditional TCM preparation process has problems such as slow onset and low bioavailability, which limit the application effect of TCM in the prevention and control of animal diseases. Therefore, it is necessary to develop a TCM formula for preventing and controlling febrile swine diseases. Summary of the Invention

[0007] The present invention provides a TCM formula for preventing and controlling febrile swine diseases and its preparation process, which solves the problems of slow onset and low bioavailability in the preparation process of TCM in related technologies.

[0008] The technical solution of the present invention is as follows: The present invention provides a TCM formula for preventing and controlling febrile swine diseases, which comprises the following raw materials in parts by weight: 30 - 50 parts of honeysuckle extract, 30 - 50 parts of wild chrysanthemum extract, 20 - 30 parts of baicalin, 30 - 50 parts of isatis root, 20 - 30 parts of salvia miltiorrhiza, 20 - 30 parts of turmeric, 30 - 50 parts of forsythia, 20 - 30 parts of gardenia, 20 - 30 parts of nacre, 5 - 10 parts of realgar, 20 - 30 parts of buffalo horn extract, 30 - 50 parts of poria cocos, 20 - 30 parts of atractylodes macrocephala, 30 - 50 parts of Chinese yam, 30 - 50 parts of coix seed, 10 - 20 parts of amomum villosum, 20 - 30 parts of platycodon grandiflorum, 10 - 20 parts of liquorice.

[0009] In the present invention, honeysuckle extract is a very valuable component. Modern research has found that it is rich in various bioactive substances such as chlorogenic acid and luteolin. These substances have powerful antiviral, antibacterial, anti - inflammatory and antioxidant effects. In the prevention and control of diseases, honeysuckle extract can effectively inhibit the replication process of various viruses. By interacting with the key proteins or nucleic acids of the virus, it destroys the structural integrity of the virus, making it lose its infectivity. Honeysuckle extract can also regulate the immune function of the body, activate immune cells, and enhance the resistance of the body, so as to better resist the invasion of diseases.

[0010] In the present invention, baicalin, as a flavonoid compound, has significant antibacterial, antiviral, anti - inflammatory and immunomodulatory effects. Research shows that baicalin can inhibit the growth and reproduction of bacteria and viruses. By inhibiting the key links such as the synthesis of bacterial cell walls, protein synthesis and viral nucleic acid replication, it exerts its antibacterial and antiviral effects. In terms of anti - inflammation, baicalin can inhibit the release of inflammatory mediators and reduce the damage of inflammatory reactions to body tissues. Its immunomodulatory effect helps to regulate the immune balance of the body, enhance the immune defense ability of the body, and improve the resistance of pigs to diseases.

[0011] In the present invention, Isatis indigotica Fort. is a commonly used traditional Chinese medicine for clearing heat and detoxifying. Its main active ingredients include indirubin, indigo, etc. These ingredients endow Isatis indigotica Fort. with good antiviral, antibacterial, anti-inflammatory and immunomodulatory effects. In the prevention and control of animal diseases, Isatis indigotica Fort. can effectively inhibit the activities of various viruses, and has significant inhibitory effects on influenza virus, herpes virus, etc. It can also enhance the immune function of the body, promote the proliferation and activity of immune cells, improve the body's immunity, and thus help pigs resist the infection of diseases.

[0012] In the present invention, Salvia miltiorrhiza Bunge is rich in various active ingredients such as tanshinone and salvianolic acid, and has the effects of promoting blood circulation to remove blood stasis, improving microcirculation, antioxidation and protecting the heart and blood vessels. In the present invention, Salvia miltiorrhiza Bunge can dilate the coronary artery, increase the coronary blood flow, provide sufficient oxygen and nutrients for the heart, improve the blood circulation of the heart, enhance the function of the heart. The antioxidation effect of Salvia miltiorrhiza Bunge can scavenge excessive free radicals in the body, reduce the damage of oxidative stress to cells, protect important cells such as myocardial cells and liver cells from damage, help maintain the normal functions of various organs of the pig body, and improve its resistance to diseases.

[0013] In the present invention, the main components of Forsythia suspensa (Thunb.) Vahl include phillyrin, forsythoside, etc., and have the effects of clearing heat and detoxifying, dissipating swelling and nodules, dispersing wind-heat, etc. In the prevention and control of diseases, Forsythia suspensa (Thunb.) Vahl can inhibit the replication and transmission of viruses, and has a good relieving effect on symptoms such as fever and inflammation caused by various virus infections. The active ingredients it contains can also enhance the immune function of the body, promote the activity of immune cells, and improve the body's immune defense ability, thus helping pigs better resist the invasion of diseases.

[0014] In the present invention, Gardenia jasminoides Ellis contains components such as geniposide and crocin, and has the effects of purging fire and relieving restlessness, clearing heat and promoting diuresis, cooling blood and detoxifying, etc. In the prevention and control of animal diseases, Gardenia jasminoides Ellis can reduce the inflammatory response, lower the body temperature, relieve symptoms such as fever and restlessness. Its effect of cooling blood and detoxifying helps to remove the heat toxin in the body, protect the body tissues from damage, and has a good therapeutic effect on symptoms such as fever and inflammation caused by virus infection, and can help pigs recover.

[0015] In the present invention, Curcuma aromatica Salisb. is rich in components such as volatile oil and curcumin, and has the effects of promoting blood circulation to relieve pain, regulating qi and relieving depression, clearing the heart and cooling blood, etc. In the present invention, Curcuma aromatica Salisb. can promote blood circulation, relieve the symptoms caused by blood stasis blockage, improve the physical condition of pigs. Its effect of regulating qi and relieving depression helps to regulate the qi mechanism of pigs, make their qi and blood unobstructed, and enhance the body's resistance. The effect of Curcuma aromatica Salisb. in clearing the heart and cooling blood can remove the heat toxin in the body, reduce the inflammatory response, and protect important organs such as the heart and liver from damage.

[0016] In the present invention, mother-of-pearl contains a variety of trace elements and amino acids, and has the effects of calming the liver and suppressing yang, clearing the liver and improving eyesight, calming the heart and tranquilizing the nerves. In epidemic prevention and control, mother-of-pearl can regulate the nervous system functions of pigs, relieve symptoms such as irritability and insomnia caused by the epidemic, keep them quiet, and be beneficial to the recovery of the body. Its effect of calming the liver and suppressing yang helps to regulate the blood pressure and physiological functions of pigs, protect the cardiovascular and cerebrovascular systems, enhance the body's resistance, and improve the pigs' tolerance to epidemics.

[0017] In the present invention, the main component of realgar is arsenic disulfide, which has the effects of detoxification, insecticide, dampness removal and expectorant. In traditional medicine, realgar is often used to treat insect and snake bites, eczema, scabies and other diseases. In the present invention, realgar can play its detoxification effect, help pigs to remove toxins in the body, and reduce the damage of epidemic diseases to the body. Its dampness removal and expectorant effect helps to improve the internal environment of pigs, prevent the endogenous generation of phlegm and dampness, and reduce the risk of epidemic diseases.

[0018] In the present invention, the buffalo horn extract has the effects of clearing away heat and cooling blood, detoxifying and calming convulsions, and its main components include cholesterol, peptides and various amino acids. When preventing and controlling epidemic diseases, the buffalo horn extract can effectively lower the body temperature of pigs, relieve high fever symptoms, and reduce inflammatory reactions. It can also enhance the body's immune function, improve the resistance of pigs, have a good therapeutic effect on symptoms such as high fever and convulsions caused by viral infections, and help the recovery of pigs.

[0019] In the present invention, Poria cocos is rich in ingredients such as Poria cocos polysaccharide, and has the effects of promoting diuresis and eliminating dampness, strengthening the spleen and calming the mind. In the present invention, Poria cocos can promote the discharge of excess water in the pig's body, alleviate edema symptoms, and improve the body's water metabolism. Its spleen-strengthening effect helps to enhance the transportation and transformation function of the spleen and stomach, promote the absorption and utilization of nutrients, and improve the physique of pigs. The calming effect of Poria cocos can regulate the nervous system function of the pig, keep it quiet, and is beneficial to the recovery of the body.

[0020] In the present invention, Atractylodes macrocephala has the effects of strengthening the spleen and replenishing qi, drying dampness and promoting diuresis, and stopping sweating. Its main components include volatile oil, atractylodes lactone, etc. In the prevention and control of animal diseases, Atractylodes macrocephala can enhance the spleen and stomach function of pigs, improve their digestion and absorption capacity, and provide sufficient nutritional support for the body, thereby enhancing the body's resistance. The drying dampness and promoting diuresis effect of Atractylodes macrocephala helps to improve the internal environment of pigs, prevent the endogenous generation of dampness, and reduce the risk of disease occurrence.

[0021] In the present invention, yam is rich in substances such as amylase and polyphenol oxidase, and has the effects of strengthening the spleen and stomach, nourishing the kidney and improving the essence. In the present invention, yam can enhance the spleen and stomach function of pigs, promote digestion and absorption, and improve the body's nutritional level. Its kidney-nourishing and essence-invigorating effects are helpful to regulate the reproductive system function of pigs, improve the reproductive performance of breeding pigs, and ensure the sustainable development of the pig industry.

[0022] In the present invention, coix seed contains ingredients such as coix seed oil and coix seed ester, and has the effects of promoting diuresis and excreting dampness, strengthening the spleen and stopping diarrhea, dispelling obstruction and discharging pus, etc. In the prevention and control of diseases, coix seed can promote the excretion of excess water in pigs, relieve edema symptoms, improve the body's water metabolism, its function of strengthening the spleen and stopping diarrhea helps to enhance the transportation and transformation function of the spleen and stomach, prevent diarrhea, and improve the physical condition of pigs. The function of coix seed in dispelling obstruction and discharging pus helps to relieve symptoms such as joint pain and swelling caused by diseases, and promote the recovery of pigs.

[0023] In the present invention, amomum fruit is rich in ingredients such as volatile oil, and has the effects of promoting digestion and regulating the stomach, warming the spleen and stopping diarrhea, regulating qi and preventing miscarriage, etc. In the present invention, amomum fruit can promote the digestive function of pigs, enhance appetite, and increase the nutrient intake. Its function of warming the spleen and stopping diarrhea helps to improve the symptoms of spleen and stomach deficiency-cold in pigs and prevent diarrhea. The function of amomum fruit in regulating qi and preventing miscarriage is particularly important for the health of breeding pigs, which can ensure the reproductive performance of breeding pigs and the normal development of fetuses.

[0024] In the present invention, platycodon root contains ingredients such as platycodin, and has the effects of dispersing the lung qi and relieving sore throat, eliminating phlegm and discharging pus, etc. In the prevention and control of animal diseases, platycodon root can promote the excretion of sputum in the respiratory tract of pigs, relieve symptoms such as cough and asthma, and improve the respiratory function. Its function of dispersing the lung qi and relieving sore throat helps to keep the respiratory tract unobstructed, reduce the risk of respiratory diseases, and improve the respiratory health level of pigs.

[0025] In the present invention, licorice contains ingredients such as glycyrrhizic acid and liquiritin, and has the effects of invigorating the spleen and replenishing qi, moistening the lungs and relieving cough, clearing heat and detoxifying, and coordinating the properties of various drugs, etc. In the present invention, licorice can enhance the function of the spleen and stomach of pigs and improve their resistance. Its function of moistening the lungs and relieving cough helps to relieve the cough symptoms caused by respiratory diseases and improve the respiratory function. The function of licorice in clearing heat and detoxifying can help pigs remove the heat toxins in the body and reduce the inflammatory reaction. Most importantly, licorice can coordinate the properties of other drugs, make various drugs work synergistically, better play the therapeutic role, and improve the overall efficacy of the traditional Chinese veterinary medicine formula for preventing and controlling febrile swine diseases.

[0026] The present invention also provides a preparation process for a traditional Chinese veterinary medicine formula for preventing and controlling febrile swine diseases, including the following steps: S1. Mix radix isatidis, salvia miltiorrhiza, curcuma aromatica, forsythia suspense, gardenia jasminoides, poria cocos, atractylodes macrocephala, Chinese yam, coix seed, amomum fruit, platycodon root, and licorice, grind and sieve them to obtain traditional Chinese medicine powder; S2. Mix honeysuckle flower extract, wild chrysanthemum flower extract, baicalin, nacre, realgar, and buffalo horn extract, grind and sieve them to obtain a mixed powder; S3. Add bovine bile liquid, the first enzyme preparation, and beneficial microorganism preparation I to the traditional Chinese medicine powder, mix evenly to obtain a mixture. After the mixture undergoes primary fermentation, steam conversion, secondary fermentation, and steam ripening, it is dried and pulverized to obtain a composite powder; S4. Mix the mixed powder, the composite powder, and the beneficial microorganism preparation II, and then sieve them to obtain the traditional Chinese veterinary medicine for preventing and controlling febrile swine diseases.

[0027] In the present invention, the bovine bile liquid has the effects of clearing heat and detoxifying, promoting bile secretion and laxation, etc. Its main components include cholic acid, deoxycholic acid, etc. These components have the effects of antibacterial, anti-inflammatory, and promoting digestion. In the present invention, the bovine bile liquid can enhance the heat-clearing and detoxifying effect of the medicine, help pigs remove the heat toxin in the body, reduce the inflammatory reaction, promote the secretion and excretion of bile, contribute to digestion and absorption, improve the nutritional intake and utilization efficiency of pigs, enhance their physique, and thus better cope with the challenges of diseases.

[0028] As a further technical solution, the preparation process of the bovine bile liquid is as follows: Add bovine bile to water I, disperse it and then sieve to obtain filtrate I and filter residue. Disperse the filter residue in water II, disperse it again and then filter to obtain filtrate II. Collect filtrate I and filtrate II, and obtain the bovine bile liquid after boiling.

[0029] As a further technical solution, the mass-to-volume ratio of the bovine bile to water I is 1 g:10 mL, the mass-to-volume ratio of the filter residue to water II is 1 g:10 mL, the mesh size of the sieve for sieving is 150 meshes, and the mass of the bovine bile liquid is 20% of the mass of the bovine bile.

[0030] In the present invention, the bovine bile is fully mixed with water to promote the dissolution and dispersion of impurities. Sieving with 150 meshes can effectively intercept larger particles of impurities. Redispersing and diluting the filter residue ensures the maximum removal of impurities in the bovine bile, providing a pure raw material for subsequent concentration and fermentation.

[0031] As a further technical solution, continuous stirring is required during the boiling process.

[0032] As a further technical solution, in step S1, the mesh size of the sieve for sieving is 60 meshes, and in step S2, the mesh size of the sieve for sieving is 100 meshes.

[0033] In the present invention, the pore size of the 60-mesh sieve can ensure that the particle size of the powder meets the requirements of subsequent processes. Larger particles are sieved out, ensuring the uniformity and consistency of the powder, which is beneficial for full mixing and reaction with other components during the subsequent fermentation process.

[0034] In the present invention, screening is carried out through a 100-mesh sieve in step S2. The 100-mesh sieve has a smaller pore size, further ensuring the fineness and uniformity of the powder, enabling these components to better play their roles in the subsequent preparations.

[0035] As a further technical solution, in step S3, the first enzyme preparation is composed of β-glucanase and pectinase with a mass ratio of 3:2, and the dosage of the first enzyme preparation is 1% of the mass of the traditional Chinese medicine powder. In steps S3 and S4, the beneficial microorganism preparation is composed of lactic acid bacteria and yeast with a mass ratio of 2:1, and the addition amount of the beneficial microorganism preparation is 1.2% of the mass of the traditional Chinese medicine powder.

[0036] In the present invention, the addition of the enzyme preparation further enhances the effect of the fermentation process. As an efficient biological catalyst, the enzyme can accelerate the progress of chemical reactions. During the fermentation process of this prescription, in addition to the enzymes secreted by the microorganisms themselves, β-glucanase and pectinase are additionally added. These enzyme preparations can cooperate with the enzymes secreted by the microorganisms and act together on the traditional Chinese medicine. β-glucanase can decompose the β-glucan component in the cell wall of the traditional Chinese medicine, and cooperate with cellulase and others to more thoroughly destroy the cell wall structure, making the release of active ingredients more sufficient. Pectinase can decompose the pectin substance in the cell wall, further improving the permeability of the cell wall and promoting the dissolution of active ingredients.

[0037] In the present invention, the beneficial microorganism preparation plays an important role in the fermentation process. They can grow and reproduce rapidly in a suitable fermentation environment, utilize the nutrients in the materials for metabolic activities. During this process, the microorganisms will secrete various enzyme substances, and these enzymes have specific catalytic effects. Lactic acid bacteria can secrete amylase, protease, etc., and yeast can produce cellulase, glucoamylase, etc. These enzymes can specifically decompose the cell wall structure of the traditional Chinese medicine. The cell wall of the traditional Chinese medicine is composed of complex substances such as cellulose, hemicellulose, and lignin, which limits the release of active ingredients inside the cell. The cellulase secreted by the microorganisms can decompose cellulose into small molecule substances such as glucose, destroying the cell wall structure and exposing and releasing the active ingredients inside the cell. Protease can decompose the protein component in the cell wall, further promoting the rupture of the cell wall and improving the release efficiency of active ingredients.

[0038] In the present invention, the enzyme preparation and the beneficial microorganism preparation are added simultaneously to exert their synergistic effect. When the active ingredients are released, the metabolic activities of the microorganisms and the catalytic action of the enzymes can prompt a series of chemical reactions to occur to these ingredients, converting them into forms that are more easily absorbed and utilized by the pig body. The metabolic products of the microorganisms, such as organic acids, vitamins, etc., can interact with the active ingredients of the traditional Chinese medicine, further changing their chemical structure and properties and improving their bioavailability and medicinal efficacy.

[0039] In the present invention, this formulation is a compound preparation of natural Chinese herbs, enzyme preparations and beneficial microorganisms, with the remarkable characteristics of being green, environmentally friendly, safe and non-toxic. In this formulation, natural Chinese herb components such as honeysuckle extract, wild chrysanthemum extract, isatis root, forsythia, etc. are all derived from plants. After strict screening and processing, their quality and safety are ensured. These natural Chinese herbs have been proven to have no obvious toxic and side effects on the animal body in long-term clinical applications, will not produce drug residues in pigs, and will not cause environmental pollution. The addition of enzyme preparations and beneficial microorganisms further enhances the efficacy of the product, and at the same time ensures its safety, and can synergistically interact with natural Chinese herb components to exert better therapeutic effects. For breeding pig farms and large and medium-sized specialized pig farms, the green and safe properties of this formulation are particularly important. Breeding pig farms need to ensure the health and reproductive performance of breeding pigs and avoid using drugs that may have adverse effects on breeding pigs. The safe and non-toxic characteristics of this formulation enable it to be safely used for the prevention and treatment of breeding pigs without damaging the reproductive system and genetic performance of breeding pigs, ensuring the quality of breeding pigs and the health of offspring. The green and environmentally friendly characteristics of this formulation prevent it from causing environmental pollution during use, which is conducive to maintaining the ecological balance and sustainable development of the pig farm. At the same time, its safe and non-toxic characteristics also reduce the risks such as pig poisoning caused by improper drug use, reducing the breeding cost and management difficulty.

[0040] As a further technical solution, in step S3, the primary fermentation is carried out in 5 times; the primary fermentation includes the following steps: subjecting the mixture to primary fermentation, drying, and grinding to obtain a first dried product; mixing the first dried product with bovine bile liquid, subjecting it to secondary fermentation, drying, and grinding to obtain a second dried product; mixing the second dried product with bovine bile liquid, subjecting it to tertiary fermentation, drying, and grinding to obtain a third dried product; mixing the third dried product with bovine bile liquid, subjecting it to quaternary fermentation, drying, and grinding to obtain a fourth dried product; mixing the fourth dried product with bovine bile liquid, subjecting it to fifth fermentation, drying, and grinding to obtain a primary fermented product.

[0041] In the present invention, through the process of five fermentations, beneficial microorganisms continuously reproduce and metabolize, and the enzyme preparation continuously decomposes and transforms Chinese herbal medicines, enabling the effective components in Chinese herbal medicines to be more fully released and utilized. The interaction between various components is more perfect, forming a stable and highly efficient symbiotic complex. This complex not only improves the bioavailability of the drug, enabling the drug to be absorbed and utilized more quickly in pigs, but also enhances the stability of the drug and the persistence of its efficacy.

[0042] As a further technical solution, in step S3, the addition amount of the bovine bile liquid is 5% of the mass of the Chinese herbal medicine powder.

[0043] As a further technical solution, during the processes of the secondary fermentation, the tertiary fermentation, the quaternary fermentation, and the quinary fermentation, the addition amount of the bovine bile liquid is 5% of the mass of the traditional Chinese medicine powder.

[0044] As a further technical solution, the bovine bile liquid is added in 5 times during each addition process.

[0045] In the present invention, to ensure the uniformity of mixing, the bovine bile is added slowly in 5 times, and after each addition, it is fully stirred to make the bovine bile contact and mix with the traditional Chinese medicine powder sufficiently.

[0046] As a further technical solution, the time of the primary fermentation, the secondary fermentation, the tertiary fermentation, the quaternary fermentation, and the quinary fermentation is 7 days each, the fermentation temperature is 40 °C each, the humidity is independently 60% - 70% each, the drying is low-temperature drying, the temperature of the low-temperature drying is 40 °C each, and the wind speed is independently 0.5 - 1.0 m / s each.

[0047] In the present invention, low-temperature drying can avoid the destruction of the effective components of the medicine by high temperature.

[0048] In the present invention, controlling the fermentation temperature at 40 °C provides a suitable environment for the growth and reproduction of beneficial microorganisms. When the temperature is too high, the enzyme activity of microorganisms may be inhibited or even inactivated, resulting in abnormal fermentation processes and affecting the transformation and generation of the effective components of the medicine. When the temperature is too high, some enzymes sensitive to temperature may lose their activity and cannot effectively decompose the cell walls of Chinese herbal medicines, thus reducing the release efficiency of the effective components. When the temperature is too low, the metabolic rate of microorganisms will be slowed down, the fermentation cycle will be prolonged, and the production efficiency will be reduced. In a low-temperature environment, the growth and reproduction rate of microorganisms slows down, and the generation amount of metabolites decreases, affecting the process and effect of fermentation.

[0049] In the present invention, by controlling the fermentation temperature, humidity, and drying ventilation conditions, the water in the fermented product slowly evaporates to reach an appropriate water content, facilitating subsequent processing.

[0050] In the present invention, during the primary fermentation, the fermentation time for each fermentation is 7 days, enabling beneficial microorganisms in the fermentation process to fully utilize the nutrients in the materials for growth and reproduction, secrete various enzyme substances, preliminarily decompose and transform Chinese herbal medicines, start the release of active ingredients and initiate preliminary chemical reactions. The purpose of multiple fermentations is to enable beneficial microorganisms to continuously reproduce and metabolize, and the enzyme preparation to continuously decompose and transform Chinese herbal medicines, so that the active ingredients in Chinese herbal medicines can be more fully released and utilized, and the interaction between components is more perfect, forming a stable and highly efficient symbiotic complex. If the fermentation time is too short, the active ingredients in the materials may not be fully released and transformed, resulting in reduced bioavailability and efficacy of the product; while if the fermentation time is too long, it may lead to excessive metabolism of beneficial microorganisms, producing some by-products that are not conducive to the product quality, and at the same time increasing production costs and time costs.

[0051] As a further technical solution, in step S3, the steam conversion includes the following steps: mixing the first-stage fermented product with bovine bile liquid, introducing steam for steam conversion, and obtaining a steam-converted product after cooling; the temperature of the steam conversion is 100 °C and the time is 24 h.

[0052] As a further technical solution, the addition amount of the bovine bile liquid is 5% of the mass of the traditional Chinese medicine powder.

[0053] In the present invention, on the one hand, high-temperature steam can further kill possible harmful microorganisms and ensure the safety of the product; on the other hand, high temperature can promote the chemical reactions between components in the materials, make them more fully fused and transformed, and improve the purity and efficacy of the medicine.

[0054] As a further technical solution, in step S3, the secondary fermentation includes the following steps: mixing the steam-converted product, bovine bile liquid and the second enzyme preparation, fermenting, drying and grinding. The fermentation time is 5 days and the temperature is 40 °C. The second enzyme preparation is composed of β-glucanase and pectinase with a mass ratio of 2:3, and the dosage of the second enzyme preparation is 0.8% of the mass of the traditional Chinese medicine powder.

[0055] In the present invention, during the secondary fermentation process, it promotes the continued growth and reproduction of residual beneficial microorganisms in a suitable environment, further promotes the fermentation and transformation of the materials, and further improves the quality of the medicine.

[0056] In the present invention, during the secondary fermentation process, β-glucanase and pectinase are added again in a ratio of 2:3 and a dosage of 0.8% of the mass of the traditional Chinese medicine powder, which can further accurately decompose substances such as complex polysaccharides, continuously optimize the fermentation process, promote the full release of active ingredients, improve the efficacy of traditional Chinese veterinary medicine, and provide more powerful guarantee for the prevention and control of febrile swine diseases.

[0057] As a further technical solution, in step S3, the temperature of the steam curing is 100°C and the time is 24 h.

[0058] In the present invention, steam curing can promote the stability of the material.

[0059] The working principle and beneficial effects of the present invention are as follows: 1. In the present invention, the effects of each component are synergistic, improving the health status of pigs from multiple aspects. In terms of heart function, the scientific combination of this formula enables various components to coordinate with each other and complement their medicinal effects. Among them, components such as Salvia miltiorrhiza can rapidly dilate the coronary artery, significantly increase the coronary blood flow, provide more sufficient oxygen and nutrients for the heart, improve the microcirculation of the heart, which helps to restore the contractility of the heart, enhance the heart's pumping function, enable the heart to more effectively transport blood to all tissues and organs of the body. At the same time, this formula can also comprehensively activate hypoxic myocardial cells, promote the massive regeneration of myocardial cells, improve the heart's hypoxia tolerance, reduce myocardial oxygen consumption, and improve myocardial energy metabolism, thereby maintaining the normal function of the heart and reducing heart damage caused by African swine fever virus infection or other factors; in enhancing cardiopulmonary function, this formula has the effects of strengthening the spleen and resolving dampness, supplementing qi and nourishing blood. By regulating the spleen and stomach function of pigs, it promotes the absorption and transportation of nutrients, provides sufficient energy and material basis for the body, and then improves and enhances cardiopulmonary function. Components such as Poria cocos, Atractylodes macrocephala, and Dioscorea opposita can strengthen the spleen and replenish qi, enhance the transportation and transformation ability of the spleen and stomach, enable the normal metabolism of water and dampness, avoid the accumulation of water and dampness in the body, and reduce the burden on the heart and lungs. Coix lacryma-jobi, Amomum villosum, etc. can resolve dampness and promote qi movement, promote the smooth flow of qi in the body, and further assist in the recovery and improvement of cardiopulmonary function. This formula can also rapidly increase urine output, promote the excretion of toxins and metabolic wastes in the body, reduce the burden on the kidneys, and indirectly protect cardiopulmonary function; for cell damage, this formula has a strong repair effect. The various components it contains can protect important organs such as the heart, brain, liver, and kidneys from damage caused by factors such as ischemia, hypoxia, peroxidation, and poisoning. Mother-of-pearl, buffalo horn extract, etc. have antioxidant and anti-inflammatory effects, can scavenge excessive free radicals in the body, reduce the damage of oxidative stress to cells, and inhibit the occurrence and development of inflammatory reactions. At the same time, these components can also promote the repair and regeneration of damaged cells, maintain the normal structure and function of cells, ensure the normal operation of each important organ, and improve the overall health level and resistance of pigs.

[0060] 2. In the present invention, this formula has a powerful inhibitory and killing effect on African swine fever virus. This formula adopts a powerful combination of antiviral traditional Chinese medicines and traditional Chinese medicine extracts. This unique formula enables it to smoothly enter the host cell body and directly act on the virus replication link. Inside the cell, this formula interferes with the synthesis process of the virus's genetic material through a variety of complex biochemical reactions, preventing the virus from using the substances and energy of the host cell for self-replication, thereby effectively inhibiting the large-scale reproduction of African swine fever virus in pigs. Each component synergizes with each other and plays a role in multiple aspects such as antiviral, antibacterial, anti-inflammatory, immune regulation, and improvement of body functions, comprehensively enhancing the resistance of pigs and effectively coping with the challenges of epidemic diseases such as febrile swine diseases. Components such as honeysuckle extract and baicalin in this formula have significant antiviral activities. Compounds such as chlorogenic acid in honeysuckle extract can interact with the protein coat or nucleic acid of the virus, destroying the structural integrity of the virus and making it lose its infectious activity; baicalin can activate the antiviral defense mechanism inside the cell by regulating the immune response signaling pathway of the host cell, enhancing the cell's resistance to the virus. The antiviral effects of components such as honeysuckle extract, baicalin, and isatis root can directly inhibit the replication and spread of the virus, reducing the invasion of the virus on pigs; the effects of promoting blood circulation to remove blood stasis and clearing heat and detoxifying of components such as salvia miltiorrhiza and forsythia suspensa help to improve the blood circulation of pigs, reduce the inflammatory response, and protect the body tissues from damage; the spleen-strengthening effects of components such as poria cocos, atractylodes macrocephala, and Chinese yam can enhance the spleen and stomach functions of pigs, improve the efficiency of nutrient absorption and utilization, provide sufficient energy and material support for the body, thereby enhancing the body's resistance; the effect of licorice in coordinating the actions of various drugs enables each component to better synergize and play a role, improving the overall therapeutic effect of the drug. Detailed implementation manners

[0061] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts fall within the scope of protection of the present invention.

[0062] In the following examples and comparative examples: Lactic acid bacteria: The effective substance content is 20 billion cfu / g; Yeast: The effective substance content is 20 billion cfu / g; β-glucanase: The enzyme activity is 50 u / mg; Pectinase: The enzyme activity is 30,000 u / g.

[0063] Example 1 A preparation process of a traditional Chinese veterinary medicine formula for preventing and controlling febrile swine diseases includes the following steps: S1. Add 100 parts of bovine bile to 1000 parts of water. After dispersion, sieve through a 150-mesh sieve to obtain filtrate Ⅰ and residue. Disperse the residue in 1000 parts of water, and after re-dispersion, filter to obtain filtrate Ⅱ. Collect filtrate Ⅰ and filtrate Ⅱ and place them in an iron pot for low-temperature heating and boiling over a low fire. Continuously stir during the boiling process. After boiling and concentrating to 20% of the original mass of bovine bile, obtain bovine bile liquid; S2. Mix 30 parts of Isatis root, 20 parts of Salvia miltiorrhiza, 20 parts of Curcuma aromatica, 30 parts of Forsythia suspensa, 20 parts of Gardenia jasminoides, 30 parts of Poria cocos, 20 parts of Atractylodes macrocephala, 30 parts of Dioscorea opposita, 30 parts of Coix lacryma-jobi, 10 parts of Amomum villosum, 20 parts of Platycodon grandiflorum, and 10 parts of Glycyrrhiza uralensis. Grind and sieve through a 60-mesh sieve to obtain traditional Chinese medicine powder; S3. Mix 30 parts of honeysuckle extract, 30 parts of wild chrysanthemum extract, 20 parts of baicalin, 20 parts of nacre, 5 parts of realgar, and 20 parts of buffalo horn extract. Grind and sieve through a 100-mesh sieve to obtain a mixed powder; S4. Add bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), the first enzyme preparation (1% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 3:2), and a beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1) to the traditional Chinese medicine powder. Mix evenly to obtain a mixture, ferment at 40°C for 7 days with a humidity of 60%. After fermentation, dry at a low temperature of 40°C to obtain the first dried product. Mix the first dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%. After fermentation, dry at a low temperature of 40°C to obtain the second dried product. Mix the second dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%. After fermentation, dry at a low temperature of 40°C to obtain the third dried product. Mix the third dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%. After fermentation, dry at a low temperature of 40°C to obtain the fourth dried product. Mix the fourth dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%. After fermentation, dry at a low temperature of 40°C to obtain the first-stage fermented product; Mix the first-stage fermented product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, and carry out steam conversion at 100°C for 24 hours. After cooling, obtain a steam-converted product. Mix the steam-converted product, bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), and the second enzyme preparation (0.8% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 2:3), ferment at 40°C for 5 days with a humidity of 60%. After fermentation, dry at a low temperature of 40°C, and then mix with concentrated bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, and carry out steam ripening at 100°C for 24 hours. After low-temperature drying at 40°C, obtain a composite powder; S5. Mix the mixed powder, the composite powder, and a beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1). After sieving through an 80-mesh sieve, obtain the traditional Chinese veterinary medicine for preventing and controlling febrile swine diseases.

[0064] Example 2 A preparation process of a traditional Chinese veterinary medicine formula for preventing and controlling epidemic febrile swine diseases, comprising the following steps: S1. Add 100 parts of bovine bile to 1000 parts of water, disperse and then pass through a 150-mesh sieve to obtain filtrate I and filter residue. Disperse the filter residue in 1000 parts of water, disperse again and then filter to obtain filtrate II. Collect filtrate I and filtrate II and place them in an iron pot for low-temperature heating and boiling over low heat. Continuously stir during the boiling process, and concentrate by boiling until it reaches 20% of the original mass of bovine bile to obtain bovine bile liquid; S2. Mix 40 parts of isatis root, 25 parts of salvia miltiorrhiza, 25 parts of curcuma aromatica, 40 parts of forsythia suspensa, 25 parts of gardenia jasminoides, 40 parts of poria cocos, 25 parts of atractylodes macrocephala, 40 parts of Chinese yam, 40 parts of coix seed, 15 parts of amomum villosum, 25 parts of platycodon grandiflorum, and 15 parts of liquorice, grind and pass through a 60-mesh sieve to obtain traditional Chinese medicine powder; S3. Mix 40 parts of honeysuckle extract, 40 parts of wild chrysanthemum extract, 25 parts of baicalin, 25 parts of nacre, 8 parts of realgar, and 25 parts of buffalo horn extract, grind and pass through a 100-mesh sieve to obtain a mixed powder; S4. Add bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), the first enzyme preparation (1% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 3:2), and a beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1) to the traditional Chinese medicine powder, mix evenly to obtain a mixture, ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, dry at a low temperature of 40°C to obtain the first dried product. Mix the first dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, dry at a low temperature of 40°C to obtain the second dried product. Mix the second dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 65%, and after fermentation, dry at a low temperature of 40°C to obtain the third dried product. Mix the third dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, dry at a low temperature of 40°C to obtain the fourth dried product. Mix the fourth dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, dry at a low temperature of 40°C to obtain the first-stage fermented product; Mix the first-stage fermented product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, and carry out steam conversion at 100°C for 24 hours, and after cooling, obtain a steam-converted product. Mix the steam-converted product, bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), and the second enzyme preparation (0.8% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 2:3), ferment at 40°C for 5 days with a humidity of 60%, and after fermentation, dry at a low temperature of 40°C, then mix with concentrated bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, and carry out steam ripening at 100°C for 24 hours, and after drying at a low temperature of 40°C, obtain a composite powder; S5. Mix the mixed powder, composite powder with a beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1). After passing through an 80-mesh sieve, a traditional Chinese veterinary medicine for preventing and controlling febrile swine diseases is obtained.

[0065] Example 3 A preparation process of a traditional Chinese veterinary medicine formula for preventing and controlling febrile swine diseases comprises the following steps: S1. Add 100 parts of bovine bile into 1000 parts of water. After dispersion, pass through a 150-mesh sieve to obtain filtrate I and filter residue. Disperse the filter residue in 1000 parts of water, and after re-dispersion, filter to obtain filtrate II. Collect filtrate I and filtrate II and place them in an iron pot for low-temperature heating and boiling over low heat. Continuously stir during the boiling process. After boiling and concentrating to 20% of the original mass of bovine bile, obtain bovine bile liquid. S2. Mix 50 parts of isatis root, 30 parts of salvia miltiorrhiza, 30 parts of turmeric, 50 parts of forsythia suspensa, 30 parts of gardenia jasminoides, 50 parts of poria cocos, 30 parts of atractylodes macrocephala, 50 parts of Chinese yam, 50 parts of coix seed, 20 parts of amomum villosum, 30 parts of platycodon grandiflorum, and 20 parts of liquorice. Grind and pass through a 60-mesh sieve to obtain traditional Chinese medicine powder. S3. Mix 50 parts of honeysuckle flower extract, 50 parts of wild chrysanthemum extract, 30 parts of baicalin, 30 parts of nacre, 10 parts of realgar, and 30 parts of buffalo horn extract. Grind and pass through a 100-mesh sieve to obtain a mixed powder. S4. Add ox bile liquid (5% of the mass of the traditional Chinese medicine powder), the first enzyme preparation (1% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 3:2), and beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1) to the traditional Chinese medicine powder, mix evenly to obtain a mixture, ferment at 40°C for 7 days with a humidity of 70%, after fermentation, conduct low-temperature drying at 40°C to obtain the first dried product, mix the first dried product with ox bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, after fermentation, conduct low-temperature drying at 40°C to obtain the second dried product, mix the second dried product with ox bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, after fermentation, conduct low-temperature drying at 40°C to obtain the third dried product, mix the third dried product with ox bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, after fermentation, conduct low-temperature drying at 40°C to obtain the fourth dried product, mix the fourth dried product with ox bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, after fermentation, conduct low-temperature drying at 40°C to obtain the first-stage fermented product; mix the first-stage fermented product with ox bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, conduct steam conversion at 100°C for 24 hours, and obtain the steam-converted product after cooling, mix the steam-converted product, ox bile liquid (5% of the mass of the traditional Chinese medicine powder), and the second enzyme preparation (0.8% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 2:3), ferment at 40°C for 5 days with a humidity of 60%, after fermentation, conduct low-temperature drying at 40°C, then mix with concentrated bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, conduct steam ripening at 100°C for 24 hours, and obtain the composite powder after low-temperature drying at 40°C; S5. Mix the mixed powder, the composite powder with the beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1), and pass through an 80-mesh sieve to obtain the traditional Chinese veterinary medicine for preventing and controlling epidemic febrile swine diseases.

[0066] Example 4 Compared with Example 1, the difference in Example 4 lies in that step S4 is different. Step S4 includes the following process: Add bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), the first enzyme preparation (1% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 3:2), and a beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1) to the traditional Chinese medicine powder, mix evenly to obtain a mixture, ferment at 40°C for 7 days with a humidity of 70%, and after fermentation, perform low-temperature drying at 40°C to obtain the first dried product. Mix the first dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C to obtain the second dried product. Mix the second dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C to obtain the third dried product. Mix the third dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C to obtain the fourth dried product. Mix the fourth dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C to obtain the first-stage fermented product; Mix the first-stage fermented product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, perform steam conversion at 100°C for 24 hours, and after cooling, perform low-temperature drying at 40°C to obtain the composite powder.

[0067] Example 5 Compared with Example 1, Example 5 is different in that step S4 is different. Step S4 includes the following process: Add bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), the first enzyme preparation (1% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 3:2), and a beneficial microorganism preparation (1.2% of the mass of the traditional Chinese medicine powder, composed of lactic acid bacteria and yeast with a mass ratio of 2:1) to the traditional Chinese medicine powder, mix evenly to obtain a mixture, ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C to obtain a first dried product. Mix the first dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C to obtain a second dried product. Mix the second dried product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), ferment at 40°C for 7 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C to obtain a first-stage fermented product; Mix the first-stage fermented product with bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, and perform steam conversion at 100°C for 24 hours, and after cooling, obtain a steam-converted product. Mix the steam-converted product, bovine bile liquid (5% of the mass of the traditional Chinese medicine powder), and a second enzyme preparation (0.8% of the mass of the traditional Chinese medicine powder, composed of β-glucanase and pectinase with a mass ratio of 2:3), ferment at 40°C for 5 days with a humidity of 60%, and after fermentation, perform low-temperature drying at 40°C, then mix with concentrated bile liquid (5% of the mass of the traditional Chinese medicine powder), blow in steam, and perform steam ripening at 100°C for 24 hours, and after low-temperature drying at 40°C, obtain a composite powder.

[0068] Example 6 Compared with Example 1, Example 6 is different in that the beneficial microorganism preparation in step S4 is replaced with an equal amount of the first enzyme preparation, and in step S4, the beneficial microorganism preparation is replaced with an equal amount of the second enzyme preparation.

[0069] Example 7 Compared with Example 1, Example 7 is different in that the first enzyme preparation in step S4 is replaced with an equal amount of the beneficial microorganism preparation, and the second enzyme preparation in step S4 is replaced with an equal amount of the beneficial microorganism preparation.

[0070] Example 8 Compared with Example 1, Example 8 is different in that the beneficial microorganism preparation in step S4 is replaced with an equal amount of the first enzyme preparation.

[0071] Example 9 Compared with Example 1, Example 9 is different in that the second enzyme preparation in step S4 is replaced with an equal amount of the first enzyme preparation.

[0072] Example 10 Compared with Example 1, Example 10 is different in that the first enzyme preparation in step S4 is replaced with an equal amount of the second enzyme preparation, and the second enzyme preparation in step S4 is replaced with an equal amount of the first enzyme preparation.

[0073] Comparative Example 1 Compared with Example 1, Comparative Example 1 is different in that forsythia is not added in step S2.

[0074] Comparative Example 2 Compared with Example 1, Comparative Example 2 is different in that poria cocos is not added in step S2.

[0075] Experimental Example 1 The traditional Chinese veterinary medicines for preventing and controlling swine febrile diseases prepared in Examples 1 to 10 and Comparative Examples 1 to 2 were subjected to a simulation experiment using an in vitro intestinal simulator. By analyzing the residues, the bioavailability of the traditional Chinese veterinary medicines for preventing and controlling swine febrile diseases was calculated.

[0076] Sample preparation: 10 g of the traditional Chinese veterinary medicines prepared in Examples 1 to 10 and Comparative Examples 1 to 2 were respectively taken and mixed into 1 kg of standard pig feed to make simulated feed to simulate the actual feeding situation.

[0077] Simulation parameter settings: Simulator type: Dynamic intestinal simulator, simulating the gastrointestinal environment of pigs; Temperature control: The temperature of the simulator is set at 39°C to simulate the normal body temperature of pigs; pH value control: The pH in the stomach is set at 2.0 - 3.0 to simulate the acidic environment of the pig's stomach; the small intestine is set at 6.0 - 7.0 to simulate the neutral environment of the pig's small intestine; the large intestine is set at 7.0 - 7.5 to simulate the weakly alkaline environment of the pig's large intestine; Digestion fluid flow rate: The flow rate in the stomach is set at 3 mL / min to simulate the secretion and flow of gastric juice; the flow rate in the small intestine is set at 5 mL / min to simulate the secretion and flow of intestinal juice; the flow rate in the large intestine is set at 2 mL / min to simulate the secretion and flow of large intestinal juice; Digestion time: The digestion time in the stomach is 2 h to simulate the residence time of food in the stomach; the digestion time in the small intestine is 4 h to simulate the digestion and absorption of food in the small intestine; the digestion time in the large intestine is 6 h to simulate the fermentation and absorption of food in the large intestine; Simulation steps: Put the simulated feed into the gastric reactor of the simulator, start the gastric simulator, set the temperature to 39°C, the pH value to 2.0 - 3.0, the flow rate to 3 mL / min, inject simulated gastric juice into the gastric reactor, start 2 hours of gastric digestion, sample every 30 minutes, record the pH value and digestion status of the gastric digestive juice. After the gastric digestion is completed, transfer the digested chyme to the small intestine simulator, set the temperature of the small intestine simulator to 39°C, the pH value to 6.0 - 7.0, the flow rate to 5 mL / min, inject simulated small intestine juice into the small intestine reactor, start 4 hours of small intestine digestion, sample every 1 hour, record the pH value and digestion status of the small intestine digestive juice. After the small intestine digestion is completed, transfer the digested chyme to the large intestine simulator, set the temperature of the large intestine simulator to 39°C, the pH value to 7.0 - 7.5, the flow rate to 2 mL / min, inject simulated large intestine juice into the large intestine reactor, start 6 hours of large intestine fermentation, sample every 2 hours, record the pH value and fermentation status of the large intestine fermentation broth.

[0078] Residue analysis: After the digestion is completed, collect the residues in the simulator, filter and dry them, and use high performance liquid chromatography (HPLC) and mass spectrometry (MS) to analyze the content of active ingredients in the residues, and calculate the bioavailability of traditional Chinese veterinary medicine.

[0079] Bioavailability (%) = [Amount of absorbed active ingredient / Initial amount of active ingredient × 100]; Amount of absorbed active ingredient: Analyze the content of active ingredients in the residues by HPLC and MS, and calculate the absorption amount; Initial amount of active ingredient: Analyze the content of active ingredients in the initial traditional Chinese veterinary medicine powder by HPLC and MS.

[0080] The test results are shown in Table 1: Table 1 Performance test results of traditional Chinese veterinary medicine for preventing and controlling swine febrile diseases prepared in Examples 1 - 10 and Comparative Examples 1 - 2

[0081] Through the simulation experiment of the in vitro intestinal simulator, the bioavailability of traditional Chinese veterinary medicine in pigs can be evaluated more accurately. The experimental results show that the traditional Chinese veterinary medicine prepared in Example 1 has a relatively high bioavailability (85.3%), indicating that its absorption effect in pigs is good, and it can effectively exert its medicinal effect to prevent and control swine febrile diseases.

[0082] Experimental Example 2 The application of the traditional Chinese veterinary medicine for preventing and controlling swine febrile diseases prepared in Example 1 is as follows: Case 1 A large pig farm is located in Hebei and has 5,000 live pigs in stock. At the initial stage of the outbreak of febrile swine diseases, the epidemic situation gradually emerged around the pig farm, and the pig farm faced a huge risk of infection. In order to effectively prevent and control the epidemic, the Chinese veterinary medicine for preventing and controlling febrile swine diseases prepared in Example 1 was mixed into the feed at a ratio of 6 kg / ton and fed to all the live pigs in the farm. After continuous feeding for 2 weeks, the incidence and health level of the live pigs were recorded within one month.

[0083] The recording results are shown in Table 2: Table 2 Application situation of Example 1

[0084] As can be seen from Case 1, after the use of the Chinese veterinary medicine for preventing and controlling febrile swine diseases, the incidence rate was effectively controlled, the mental state was significantly improved, the feeding and drinking returned to normal, the growth and development were effectively guaranteed, the fur of the pigs became brighter, the physique was enhanced, and the overall health level was significantly improved.

[0085] Case 2 A certain pig farm in Sichuan is mainly engaged in the breeding and sales of breeding pigs, with 1,000 breeding pigs in stock. During the epidemic of febrile swine diseases, the Chinese veterinary medicine for preventing and controlling febrile swine diseases prepared in Example 1 was mixed into the breeding pig feed at a ratio of 6 kg / ton during the daily feeding. After insisting on feeding for one month, the breeding pigs were physically examined and disease detected, and the health conditions of the breeding pigs were recorded.

[0086] The recording results are shown in Table 3: Table 3 Application situation of Example 1

[0087] As can be seen from Case 2, after the use of the Chinese veterinary medicine for preventing and controlling febrile swine diseases, in the severe situation of the epidemic of febrile swine diseases, no case of febrile swine disease infection occurred in the breeding pig farm, the health condition of the breeding pig group was good, the reproductive performance of the breeding pigs was effectively guaranteed, it could not only effectively prevent the infection of febrile swine diseases, but also had no adverse effect on the reproductive performance of the breeding pigs. Instead, it helped to maintain the health of the breeding pigs, improve the reproductive efficiency, the immunity of the breeding pigs was enhanced, the resistance to other common diseases was also improved, the incidence rates of respiratory diseases, digestive tract diseases, etc. of the breeding pigs were significantly reduced, the culling rate of breeding pigs caused by diseases was reduced, and the production efficiency of the breeding pig farm was further guaranteed.

[0088] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A Chinese veterinary medicine formula for preventing and controlling swine plague, characterized in that: The invention comprises the following raw materials in parts by weight: 30-50 parts of honeysuckle extract, 30-50 parts of wild chrysanthemum extract, 20-30 parts of baicalin, 30-50 parts of isatis root, 20-30 parts of salvia miltiorrhiza, 20-30 parts of curcuma, 30-50 parts of forsythia, 20-30 parts of gardenia, 20-30 parts of mother of pearl, 5-10 parts of realgar, 20-30 parts of buffalo horn extract, 30-50 parts of poria, 20-30 parts of atractylodes, 30-50 parts of yam, 30-50 parts of coix seed, 10-20 parts of amomum, 20-30 parts of platycodon, and 10-20 parts of licorice.

2. The preparation process of a Chinese veterinary medicine prescription for preventing and controlling plague swine disease according to claim 1, characterized in that: The following steps are involved: S1. Mix Radix Isatidis, Radix Salviae Miltiorrhizae, Radix Curcumae, Fructus Forsythiae, Fructus Gardeniae, Poria, Atractylodes Macrocephalae, Rhizoma Dioscoreae, Fructus Coix, Fructus Amomi, Platycodon Grandiflorum and Radix Glycyrrhizae, grind and sieve to obtain Chinese medicine powder; S2, mixing honeysuckle extract, wild chrysanthemum extract, baicalin, mother of pearl, realgar, and buffalo horn extract, grinding and sieving to obtain a mixed powder; S3, adding ox bile liquid, the first enzyme preparation and the beneficial microorganism preparation I to the Chinese medicine powder, mixing them uniformly to obtain a mixture, and subjecting the mixture to primary fermentation, steam conversion, secondary fermentation, steam ripening, drying and pulverizing to obtain a composite powder; S4. The mixed powder, the composite powder and the beneficial microbial preparation II are mixed and sieved to obtain a Chinese veterinary medicine for preventing and controlling plague swine disease.

3. The preparation process of a Chinese veterinary medicine prescription for preventing and controlling plague swine disease according to claim 2, characterized in that: The preparation process of the ox bile liquid is as follows: adding ox bile into water I, dispersing and sieving to obtain filtrate I and filter residue, dispersing the filter residue in water II, dispersing again and filtering to obtain filtrate II, collecting filtrate I and filtrate II, and boiling to obtain ox bile liquid.

4. The preparation process of a Chinese veterinary medicine prescription for preventing and controlling plague swine disease according to claim 2, characterized in that: In step S1, the specification of the sieve is 60 mesh, and in step S2, the specification of the sieve is 100 mesh.

5. The preparation process of a Chinese veterinary medicine prescription for preventing and controlling plague swine disease according to claim 2, characterized in that: In step S3, the first enzyme preparation is composed of β-glucanase and pectinase in a mass ratio of 3:2, and the amount of the first enzyme preparation is 1% of the mass of the traditional Chinese medicine powder. In step S4, the beneficial microbial preparation I is composed of lactic acid bacteria and yeast in a mass ratio of 2:1, and the amount of the beneficial microbial preparation I added is 1.2% of the mass of the traditional Chinese medicine powder.

6. The preparation process of a Chinese veterinary medicine prescription for preventing and controlling plague swine disease according to claim 2, characterized in that: In step S3, the primary fermentation is performed in 5 times; the primary fermentation includes the following steps: fermenting, drying and grinding the mixture once to obtain a first dried product; mixing the first dried product with cattle bile juice, fermenting, drying and grinding twice to obtain a second dried product; mixing the second dried product with cattle bile juice, fermenting, drying and grinding three times to obtain a third dried product; mixing the third dried product with cattle bile juice, fermenting, drying and grinding four times to obtain a fourth dried product; mixing the fourth dried product with cattle bile juice, fermenting, drying and grinding five times to obtain a first-stage fermented product.

7. The preparation process of a Chinese veterinary medicine prescription for preventing and controlling swine plague according to claim 6, characterized in that: The time for the first fermentation, the second fermentation, the third fermentation, the fourth fermentation and the fifth fermentation are all 7 days, the fermentation temperature is all 40°C, and the drying is all low-temperature drying, and the temperature of the low-temperature drying is 40°C.

8. The preparation process of a Chinese veterinary medicine prescription for preventing and controlling plague swine disease according to claim 2, characterized in that: In step S3, the steam conversion includes the following steps: mixing the first-stage fermentation product with cattle bile liquid, blowing steam into it, performing steam conversion, and obtaining a steam conversion product after cooling; the temperature of the steam conversion is 100° C. and the time is 24 hours.

9. The preparation process of a Chinese veterinary medicine formula for preventing and controlling swine plague according to claim 2, characterized in that: In step S3, the secondary fermentation includes the following steps: mixing the steam conversion product, cow bile liquid and the second enzyme preparation, fermenting, drying and grinding, the fermentation time is 5 days, the temperature is 40°C, the second enzyme preparation is composed of β-glucanase and pectinase in a mass ratio of 2:3, and the amount of the second enzyme preparation is 0.8% of the mass of the traditional Chinese medicine powder.

10. The preparation process of a Chinese veterinary medicine formula for preventing and controlling swine plague according to claim 2, characterized in that: In step S3, the steam aging temperature is 100° C. and the time is 24 hours.

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