Spina date seed paste taking gamma-aminobutyric acid and tea leaf theanine as raw materials and preparation process

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to spina date seed paste prepared from gamma-aminobutyric acid and theanine in tea leaves as raw materials and a preparation process of the spina date seed paste. The spina date seed paste is prepared from at least one of spina date seeds, longan aril, raspberries, lotus seeds, lilies, smoked plums, mulberries, jujubes, gamma-aminobutyric acid, poria cocos, tea leaf theanine, fermented soybeans, dried ginger and auxiliaries. The preparation method comprises the following steps: weighing the raw materials, cleaning, pouring the raw materials and water into a vacuum concentration extraction tank, performing negative pressure, performing sterile soaking, performing primary extraction and performing secondary extraction: after the primary extraction, putting medicine residues into the extraction tank again, adding water, performing extraction, and after the extraction is finished, combining two extracting solutions; concentrating: further concentrating the combined extracting solution by using a vacuum concentration and extraction tank until clear paste is obtained; and mixing the prepared clear paste, refined honey, gamma-aminobutyric acid, tea leaf theanine and water, uniformly stirring, and decocting to obtain a finished product. According to the invention, the traditional paste formula and the skill of the spina date seed paste can be deeply optimized.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine drugs, and specifically relates to a wild jujube seed paste and a preparation process using γ-aminobutyric acid and theanine as raw materials. Background Art

[0002] In the Qing Dynasty, the physicians of the Wumen Medical School were good at using the wild jujube seed decoction to treat insomnia. The original formula of the wild jujube seed decoction was "two liters of wild jujube seeds, one liang of licorice, two liang of Anemarrhena asphodeloides, two liang of Poria cocos, and two liang of Ligusticum wallichii". There have been many improvements to the wild jujube seed formula in modern times. For example: (1) The patent application with the publication number CN118512567A discloses a traditional Chinese medicine composition for treating insomnia, and the raw materials include: roasted licorice, Poria cocos, ginger, cinnamon twig, wild jujube seeds, longan, lotus seed core, lily, ginseng, bamboo leaf, dragon bone, Ophiopogon japonicus, white peony root, angelica, polygala tenuifolia, Acorus tatarinowii, and Poria cum Radix Pini. (2) Some scholars only studied the compounding of the extracts of wild jujube seeds, theanine, and γ-aminobutyric acid (GABA), and found that the combined use of these three can shorten the sleep latency of mice, prolong the sleep time of mice, and increase the incidence of mice falling asleep.

[0003] The Li Liangji wild jujube seed good night paste on the market is based on the ancient formula, with wild jujube seeds as the main formula. According to the physical characteristics of modern people, raw materials such as longan pulp, raspberry, lotus seed, lily, dark plum, mulberry, jujube, Poria cocos, and semen sojae preparatum are selected and matched, and GABA and theanine are additionally added, and it is boiled and made into paste through traditional paste-making techniques. The preparation process of traditional paste usually includes boiling, decocting, extracting juice, concentrating Chinese medicinal materials, and adding sugar-based auxiliary materials such as honey and xylitol to make a paste-like medicine. On this basis, how to deeply optimize the traditional paste and its techniques of wild jujube seed paste is a difficult problem. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the present invention provides a wild jujube seed paste and a preparation process using γ-aminobutyric acid and theanine as raw materials.

[0005] The technical solution of the present invention is as follows: A wild jujube seed paste using γ-aminobutyric acid and theanine as raw materials, and the raw materials of the wild jujube seed paste include at least one of wild jujube seeds, longan pulp, raspberry, lotus seed, lily, dark plum, mulberry, jujube, γ-aminobutyric acid, Poria cocos, theanine, semen sojae preparatum, dried ginger, and auxiliary agents.

[0006] Preferably, the auxiliary agents include at least one of citric acid, ergothioneine, phycocyanin, and refined honey.

[0007] Preferably, by weight, the raw materials of the wild jujube seed paste include 5 to 60 parts of wild jujube seeds, 10 to 50 parts of longan pulp, 1 to 50 parts of raspberry, 1 to 50 parts of lotus seeds, 3 to 50 parts of lily, 2 to 50 parts of dark plum, 3 to 50 parts of mulberry, 3 to 50 parts of Chinese date, 0.1 to 10 parts of γ-aminobutyric acid, 0.1 to 10 parts of Poria cocos, 0.1 to 10 parts of theanine, 0.1 to 10 parts of fermented soybean, 0.1 to 10 parts of dried ginger, and at least one of 1 to 60 parts of an auxiliary agent.

[0008] Further, by weight, the weight ratio of ergothioneine to phycocyanin is 1:0.5 to 2.

[0009] A preparation method of the wild jujube seed paste using γ-aminobutyric acid and theanine as raw materials includes the following steps: (1) Weigh the raw materials: wild jujube seeds, longan pulp, raspberry, lotus seeds, lily, dark plum, mulberry, Chinese date, γ-aminobutyric acid, Poria cocos, theanine, fermented soybean, dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and water into an extraction tank, set the negative pressure intensity in the container at 0.5 to 0.9 atm, 25°C to 30°C, and soak in a sterile environment; (2) Primary extraction: Extract the soaked raw materials, add water for extraction to obtain medicinal residues and an extract; (3) Secondary extraction: After the primary extraction, put the medicinal residues into the extraction tank again, add water, extract, and after the extraction is completed, combine the two extracts; (4) Concentration: Further concentrate the combined extract using an extraction tank until a clear paste is obtained; (5) Mix the prepared clear paste, refined honey, γ-aminobutyric acid, theanine, and water, stir well, and simmer over low heat to obtain the wild jujube seed paste.

[0010] Preferably, it includes the following steps: (1) Weigh the raw materials: wild jujube seeds, longan pulp, raspberry, lotus seeds, lily, dark plum, mulberry, Chinese date, γ-aminobutyric acid, Poria cocos, theanine, fermented soybean, dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and water into an extraction tank, set the negative pressure intensity in the container at 0.5 to 0.9 atm, 25°C to 30°C, and soak in a sterile environment.

[0011] (2) Primary extraction: Extract the soaked raw materials, add purified water 5 to 12 times the weight of the raw materials, control the extraction temperature at 50°C to 60°C, and extract to obtain medicinal residues and an extract; (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank, add purified water at 5 - 12 times the weight of the medicinal residues, control the extraction temperature at 50 - 60°C, and conduct the extraction; after the extraction is completed, combine the two extraction liquids. (4) Concentration: Under the condition of 50 - 60°C, further concentrate the combined extraction liquid using the extraction tank until the relative density of the clear paste is 1.4 - 1.5 to obtain the clear paste. (5) Mix the prepared clear paste, refined honey, γ-aminobutyric acid, theanine, and water, stir well, and then simmer over low heat to obtain the wild jujube seed paste.

[0012] A preparation method of the wild jujube seed paste using γ-aminobutyric acid and theanine as raw materials includes the following steps: (1) Weigh the raw materials: wild jujube seeds, longan pulp, raspberry, lotus seed, lily, dark plum, mulberry, Chinese date, γ-aminobutyric acid, poria cocos, theanine, fermented soybean, dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, then pour all the raw materials including γ-aminobutyric acid and theanine and water into the extraction tank, set the negative pressure intensity in the container at 0.5 - 0.9 atm, at 25°C - 30°C, and soak in a sterile environment. (2) Primary extraction: Extract the soaked raw materials, add water for extraction to obtain medicinal residues and an extraction liquid. (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank, add water for extraction, and after the extraction is completed, combine the two extraction liquids. (4) Concentration: Further concentrate the combined extraction liquid using the extraction tank to obtain the clear paste. (5) Mix the prepared clear paste, refined honey, and water, stir well, and then simmer over low heat to obtain the wild jujube seed paste.

[0013] Preferably, it includes the following steps: (1) Weigh the raw materials: wild jujube seeds, longan pulp, raspberry, lotus seed, lily, dark plum, mulberry, Chinese date, γ-aminobutyric acid, poria cocos, theanine, fermented soybean, dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, then pour all the raw materials including γ-aminobutyric acid and theanine and water into the extraction tank, set the negative pressure intensity in the container at 0.5 - 0.9 atm, at 25°C - 30°C, and soak in a sterile environment. (2) Primary extraction: Extract the soaked raw materials, add water for extraction to obtain medicinal residues and an extraction liquid. (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank, add water for extraction, and after the extraction is completed, combine the two extraction liquids. (4) Concentration: Further concentrate the combined extraction liquid using the extraction tank to obtain the clear paste. (5) Mix the prepared clear extract, refined honey, γ-aminobutyric acid, theanine, and water, stir well, and then simmer over low heat to obtain the wild jujube seed paste.

[0014] A method for preparing the wild jujube seed paste using γ-aminobutyric acid and theanine as raw materials comprises the following steps: (1) Weigh the raw materials: wild jujube seeds, longan pulp, raspberry, lotus seed, lily, smoked plum, mulberry, Chinese date, γ-aminobutyric acid, poria cocos, theanine, fermented soybean, dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, then pour the other raw materials and water into the extraction tank, and set the negative pressure intensity in the container to 0.5 - 0.9 atm, the temperature to 25°C - 30°C, and soak in a sterile environment; (2) Primary extraction: Extract the soaked raw materials, add water for extraction to obtain the medicinal residues and the extract; (3) Secondary extraction: After the primary extraction, put the medicinal residues into the extraction tank again, add water for extraction. After the extraction is completed, combine the two extracts to obtain the combined extract; (4) Decocting: Conduct the first decoction: Add water and / or smoked plum to the medicinal residues for decocting, filter the decoction through a sieve, and then conduct the second decoction: Add water to the decoction and continue decocting, filter the decoction through a sieve, and combine the two decoctions and the combined extract to obtain the combined decoction; (5) Concentration: Further concentrate the combined decoction using the extraction tank to obtain a clear extract; (6) Mix the prepared clear extract, refined honey, γ-aminobutyric acid, theanine, and water, stir well, and then simmer over low heat to obtain the wild jujube seed paste.

[0015] A method for preparing the wild jujube seed paste using γ-aminobutyric acid and theanine as raw materials comprises the following steps: (1) Weigh the raw materials: wild jujube seeds, longan pulp, raspberry, lotus seed, lily, smoked plum, mulberry, Chinese date, γ-aminobutyric acid, poria cocos, theanine, fermented soybean, dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, then pour the other raw materials and water into the extraction tank, and set the negative pressure intensity in the container to 0.5 - 0.9 atm, the temperature to 25°C - 30°C, and soak in a sterile environment; (2) Primary extraction: Extract the soaked raw materials, add water, and then add citric acid to adjust the pH of the solution to 4.0 - 4.5, and extract to obtain the medicinal residues and the extract; (3) Secondary extraction: After the primary extraction, put the medicinal residues into the extraction tank again, add water for extraction. After the extraction is completed, combine the two extracts; (4) Concentration: Further concentrate the combined extract using the extraction tank to obtain a clear extract; (5) Mix the prepared clear extract, refined honey, γ-aminobutyric acid, theanine, and water, stir well, and then simmer over low heat to obtain the wild jujube seed paste.

[0016] A method for preparing the wild jujube seed paste using γ-aminobutyric acid and theanine as raw materials includes the following steps: (1) Weigh the raw materials: wild jujube seeds, longan pulp, raspberry, lotus seed, lily, smoked plum, mulberry, Chinese date, γ-aminobutyric acid, Poria cocos, theanine, fermented soybean, and dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and water into the extraction tank. Set the negative pressure intensity in the container at 0.5 - 0.9 atm, the temperature at 25°C - 30°C, and soak in a sterile environment. (2) Primary extraction: Extract the soaked raw materials by adding water to obtain the medicinal residues and the extract. (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank, add water, and extract. After the extraction is completed, combine the two extracts. (4) Concentration: Further concentrate the combined extract using the extraction tank to obtain a clear extract. (5) Mix the prepared clear extract, refined honey, γ-aminobutyric acid, theanine, water and / or ergothioneine and / or phycocyanin, stir well, and then simmer over low heat to obtain the wild jujube seed paste.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows: First, multiple technical means of the present application cooperate with each other. The improved technical means include: 1) Negative pressure sterile soaking: Before the primary extraction, pour the medicinal materials and water into the extraction tank, and set the negative pressure intensity in the container at 0.5 - 0.9 atm, the temperature at 25°C - 30°C, and soak in a sterile environment. 2) Optimize the medicinal material formula during negative pressure sterile soaking. 3) Optimize the extraction process, add primary extraction and secondary extraction, and optimize their parameters. 4) Optimize the medicinal material formula of the extraction process. 5) Optimize the medicinal material formula during the final simmering. 6) Add the decoction process after the secondary extraction. 7) Optimize the medicinal material formula of the decoction process. Multiple technical means of the present application cooperate with each other to successfully and deeply optimize the traditional paste formula and its techniques of the wild jujube seed paste, and improve the content of active ingredients.

[0018] Second, according to the characteristics of different medicinal material components, process parameters such as the water consumption, extraction time, and decoction time are adjusted to ensure the maximum and uniform release of each component, making the paste formula have higher medicinal efficacy stability. The present application also optimizes the formula, which not only ensures the full release of the core active ingredients but also introduces a variety of synergistic auxiliaries to enhance the comprehensive ability of the paste formula.

[0019] Thirdly, this application adds negative-pressure sterile soaking: Before the initial extraction, the medicinal materials and water are poured into the extraction tank, and the negative-pressure intensity in the container is set to 0.5~0.9 atm, at 25°C~30°C, and soaked in a sterile environment. This process can reduce the oxidation and volatilization of components under lower temperature and negative-pressure conditions, and at the same time can promote the slight fermentation of the medicinal materials and the release of active ingredients. This application also performs extraction twice. The double extraction enables the full release of the active ingredients in the medicinal materials. Especially for multiple Chinese medicinal materials in a compound formula, this two-time extraction ensures the maximization of extraction efficiency.

[0020] Fourthly, sour jujube seeds are rich in γ-aminobutyric acid. During the co-decoction process, smoked plum is added to adjust the pH of the solution to increase the dissolution of the active ingredients of the medicinal materials. At the same time, citric acid in smoked plum destroys jujuboside A in sour jujube seeds and increases the amount of jujubogenin. Jujuboside A contains more sugar ligands and is difficult to penetrate the blood-brain barrier, while jujubogenin can penetrate the blood-brain barrier and play a sedative effect.

[0021] Fifthly, through the synergistic effect of the components in the sour jujube seed paste, the prescription can not only effectively improve insomnia and anxiety symptoms, but also has comprehensive effects of supplementing qi and nourishing blood, regulating emotions, and improving physique. These synergistic effects make the sour jujube seed paste produce better effects than single components in many aspects, ensuring the multifunctionality and effectiveness of the prescription and meeting the health needs of modern people. Detailed implementation mode

[0022] The technical solution of the present invention will be further elaborated below in conjunction with the embodiments, but the scope protected by the present invention is not limited thereto.

[0023] (1) Optimize the preparation method of the sour jujube seed paste Comparative example 1 For the existing preparation method of the sour jujube seed paste, the raw materials refer to the prescription of the sour jujube seed good-night paste of Li Liangji. The traditional paste-making technique and the weight parts of the raw materials refer to the patent application with the publication number CN114868895A. It should be noted that the sour jujube seed good-night paste of Li Liangji can be obtained through regular purchase from a commercial company, and its prescription can be known to the public. In addition, the above reference does not mean exactly the same, and adjustments are made according to the actual situation in this comparative example.

[0024] The specific preparation steps are as follows: (1) Weigh the raw materials: Weigh 8 parts of sour jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese dates, 0.8 part of γ-aminobutyric acid, 4 parts of poria cocos, 0.6 part of theanine, 2 parts of semen sojae praeparatum, and 2 parts of dried ginger according to weight parts. Except for γ-aminobutyric acid and theanine, the other raw materials are purified, and the other raw materials are soaked in 10 times the amount of water for 1 h to obtain the soaked raw materials.

[0025] (2) Extract the soaked raw materials twice at 70 °C for 2 hours each time, adding 10 times and 8 times the amount of water of the raw materials respectively. After the extraction, concentrate to a clear extract with a density of 1.2 (measured at 50 °C).

[0026] (3) Mix the prepared clear extract, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, and 10 times the amount of water of them, stir well and then simmer over low heat to obtain the wild jujube seed paste.

[0027] The following is the improvement of the process of Comparative Example 1: Example 1 A method for preparing wild jujube seed paste, the specific preparation steps are as follows: (1) Weigh the raw materials: Weigh 8 parts of wild jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese date, 0.8 part of γ-aminobutyric acid, 4 parts of poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and 10 times the purified water into a vacuum concentration extraction tank, set the negative pressure intensity in the container to 0.5 atm, at 25 °C - 30 °C, and soak for 4 hours in a sterile environment.

[0028] The following continues the primary extraction, secondary extraction and concentration in the vacuum concentration extraction tank, maintaining the negative pressure intensity in the container at 0.5 atm: (2) Primary extraction: Extract the soaked raw materials, add 10 times the weight of the raw materials of purified water, control the extraction temperature at 50 °C, and the extraction time is 2 hours to obtain medicinal residues and an extract.

[0029] (3) Secondary extraction: After the primary extraction, put the medicinal residues into the extraction tank again, add 8 times the weight of the medicinal residues of purified water, control the extraction temperature at 50 °C as well, and the extraction time is 2 hours; after the extraction, combine the two extracts.

[0030] (4) Concentration: Under the condition of 50 °C, further concentrate the combined extract using a vacuum concentration extraction tank until the relative density of the clear extract is 1.4 (measured at 30 °C) to obtain a clear extract.

[0031] (5) Mix the prepared clear extract, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, and 10 times the purified water of them, stir well and then simmer over low heat to obtain the wild jujube seed paste.

[0032] It should be noted that this application adds negative-pressure aseptic soaking: before the initial extraction, the medicinal materials and water are poured into the extraction tank, and the negative-pressure intensity in the container is set to 0.5 - 0.9 atm, at 25°C - 30°C, and soaked in a sterile environment. This process can reduce the oxidation and volatilization of components under lower temperature and negative-pressure conditions, and at the same time can promote the slight fermentation of the medicinal materials and the release of active ingredients. This application also performs extraction twice. The double extraction enables the full release of the active ingredients in the medicinal materials. Especially for various Chinese medicinal materials in a compound formula, this two-time extraction ensures the maximization of the extraction efficiency.

[0033] Mechanism of action: 1) Negative-pressure soaking: Under negative pressure, the penetration power of water is enhanced, which can prompt the active ingredients in the medicinal materials (such as jujuboside and GABA) to dissolve in water faster. Negative pressure can also reduce the air content inside the medicinal materials, accelerate the infiltration of water, and improve the leaching efficiency of active ingredients. 2) Gentle extraction (25°C - 30°C): This relatively low temperature can effectively avoid the degradation of thermosensitive components (such as GABA and jujubogenin), ensuring the integrity of the active ingredients in the extract. 3) It has the effect of slight fermentation. Chinese blackberry contains superoxide dismutase SOD, which can enzymatically hydrolyze part of the jujuboside leached out by water soaking. The molecular structure of jujuboside A is relatively complex, containing glycosyl ligands, which are difficult to be absorbed by the human body and penetrate the blood-brain barrier. However, jujubogenin can be generated by hydrolyzing glycosyl ligands under the action of fermentation, having better bioavailability and penetrability, and being able to more easily enter the brain to play a sedative role. During the extraction process, SOD enzyme promotes the transformation of saponin compounds in jujube seeds, converting the originally difficult-to-absorb jujuboside A into highly active jujubogenin, thus enhancing the efficacy of the medicinal paste. 4) The process of slight fermentation can promote the rupture of plant cell walls, releasing more γ-aminobutyric acid. Through low-temperature extraction, it can ensure that GABA is not destroyed by high temperature during the fermentation process, thus retaining a high content of GABA in the medicinal paste. Combining the combined action of SOD in Chinese blackberry and other active ingredients of traditional Chinese medicine, it promotes the accumulation of GABA, thereby improving the sedative and sleep-aiding effects of the medicinal paste. 5) During the whole soaking and extraction process, this application uses a sterile environment, which not only ensures that the medicinal materials are not contaminated by microorganisms during the slight fermentation process, but also improves the shelf life and stability of the medicinal paste.

[0034] Example 2 The preparation method of jujube seed paste is as follows: (1) Weigh the raw materials: Weigh 8 parts by weight of wild jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese date, 0.8 part of γ-aminobutyric acid, 4 parts of poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and 10 times the amount of purified water into a vacuum concentration extraction tank. Set the negative pressure intensity in the container to 0.5 atm, soak at 25°C - 30°C in a sterile environment for 4 hours.

[0035] Next, continue with the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining a negative pressure intensity of 0.5 atm in the container: (2) Primary extraction: Extract the soaked raw materials, add 6 times the weight of the raw materials of purified water, control the extraction temperature at 50°C, and the extraction time is 2 hours to obtain medicinal residues and extract.

[0036] (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank again, add 6 times the weight of the medicinal residues of purified water, control the extraction temperature at 50°C as well, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts.

[0037] (4) Concentration: Filter the combined extract through a 500-mesh sieve, and then use the vacuum concentration extraction tank to further concentrate at 50°C until the relative density of the clear paste is 1.4 (measured at 30°C) to obtain the clear paste.

[0038] (5) Mix the prepared clear paste, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, and 10 times the amount of purified water, stir well, and then simmer at a low heat of 50°C - 60°C to obtain wild jujube seed paste.

[0039] It should be noted that in this application, the extraction process is further optimized by adding primary extraction and secondary extraction and optimizing their parameters. 1) The water consumption is further optimized. Using 6 times the amount of water for two extractions, compared with 10 times and 8 times the amount of water in Example 1 and Comparative Example 1, the reduction in water volume not only improves the extraction efficiency but also shortens the time required for the concentration step, saving energy consumption. It is found that reducing the volume of the extract instead increases the concentration of the active ingredients in the medicinal materials, thereby increasing the retention rate of the active ingredients in the medicinal paste. 2) A filtration step is added during concentration: Adding a 500-mesh sieve filtration and precisely controlling the simmering temperature ensure the quality of the medicinal paste and the retention of the active ingredients, and the quality and active ingredient content of the medicinal paste are more guaranteed.

[0040] Example 3 The preparation method of wild jujube seed paste is as follows: (1) Weigh the raw materials: Weigh 8 parts of wild jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese date, 0.8 part of γ-aminobutyric acid, 4 parts of Poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger by weight. Except for γ-aminobutyric acid and theanine, purify the other raw materials, and then pour all the raw materials including γ-aminobutyric acid and theanine and 10 times of purified water into a vacuum concentration extraction tank. Set the negative pressure intensity in the container to 0.5 atm, the temperature to 25°C - 30°C, and soak for 4 hours in a sterile environment.

[0041] Next, continue with the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining a negative pressure intensity of 0.5 atm in the container: (2)Primary extraction: Extract the soaked raw materials, add 10 times the weight of purified water of the raw materials, control the extraction temperature at 50°C, and the extraction time is 2 hours to obtain medicinal residues and an extract.

[0042] (3)Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank again, add 8 times the weight of purified water of the medicinal residues, control the extraction temperature at 50°C as well, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts.

[0043] (4)Concentration: Under the condition of 50°C, further concentrate the combined extract using a vacuum concentration extraction tank until the relative density of the clear paste is 1.4 (measured at 30°C) to obtain a clear paste.

[0044] (5)Mix the prepared clear paste, 30 parts of refined honey, and 10 times of purified water, stir well, and simmer at a low heat of 50°C - 60°C to obtain wild jujube seed paste.

[0045] It should be noted that the medicinal material formula during negative pressure sterile soaking is further optimized. Add γ-aminobutyric acid and theanine during soaking to make them participate in the soaking and extraction throughout the process. This method ensures that both components can participate in the release of the active ingredients of the medicinal materials during the entire extraction process and are fully mixed with other components in the extract. The early addition of γ-aminobutyric acid and theanine can better promote the interaction between them and the active ingredients, enhancing the comprehensive efficacy of the medicinal paste. At the same time, this application ensures that all components can be released at a mild temperature during the entire extraction process.

[0046] Example 4 The preparation method of wild jujube seed paste is as follows: (1) Weigh the raw materials: Weigh 8 parts of wild jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese date, 1.6 parts of γ-aminobutyric acid, 4 parts of Poria cocos, 1.2 parts of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger by weight. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, the other raw materials, and 10 times of purified water into a vacuum concentration extraction tank. Set the negative pressure intensity in the container to 0.5 atm, soak at 25°C - 30°C in a sterile environment for 4 hours.

[0047] Next, continue with the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining a negative pressure intensity of 0.5 atm in the container: (2)Primary extraction: Extract the soaked raw materials, add 10 times the weight of the raw materials of purified water, control the extraction temperature at 50°C, and the extraction time is 2 hours to obtain medicinal residues and an extract.

[0048] (3)Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank again, add 8 times the weight of the medicinal residues of purified water, control the extraction temperature at 50°C as well, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts.

[0049] (4)Concentration: Under the condition of 50°C, use the vacuum concentration extraction tank to further concentrate the combined extract until the relative density of the clear paste is 1.4 (measured at 30°C) to obtain a clear paste.

[0050] (5)Mix the prepared clear paste, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, and 10 times of purified water, stir well, and then simmer at 50°C - 60°C over low heat to obtain wild jujube seed paste.

[0051] It should be noted that in the final simmering of this application, 0.8 part of γ-aminobutyric acid and 0.6 part of theanine are also added. By increasing the content of γ-aminobutyric acid and theanine, and the method of adding them step by step, it can not only promote the sedative effect, but also synergistically promote the conversion of jujuboside A to sapogenin. The increased content of γ-aminobutyric acid and theanine can further improve the release of active ingredients during the fermentation process, and produce a synergistic effect with other ingredients to improve the bioavailability of the medicinal materials.

[0052] Example 5 The preparation method of wild jujube seed paste, adding a decoction process, and the specific preparation steps are as follows: (1) Weigh the raw materials: Weigh 8 parts of wild jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese date, 0.8 part of γ-aminobutyric acid, 4 parts of poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and 10 times the purified water into a vacuum concentration extraction tank. Set the negative pressure intensity in the container to 0.5 atm, soak at 25°C - 30°C in a sterile environment for 4 hours.

[0053] Next, continue the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining a negative pressure intensity of 0.5 atm in the container: (2) Primary extraction: Extract the soaked raw materials, add 10 times the purified water by weight of the raw materials, control the extraction temperature at 50°C, and the extraction time is 2 hours to obtain medicinal residues and an extract.

[0054] (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank again, add 8 times the purified water by weight of the medicinal residues, control the extraction temperature at 50°C as well, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts to obtain a combined extract.

[0055] (4) Decoction: Conduct the first decoction: Immerse the medicinal residues in purified water, bring to a boil, filter the decoction through a 500-mesh sieve, and then conduct the second decoction: Add 1 time of water to the decoction and continue to boil until it boils, filter the decoction through a 500-mesh sieve, combine the two decoctions and the previously combined extract to obtain a combined decoction.

[0056] (4) Concentration: Under the condition of 55°C, further concentrate the combined decoction using a vacuum concentration extraction tank until the relative density of the clear paste is 1.5 (measured at 25°C) to obtain a clear paste.

[0057] (5) Mix the prepared clear paste, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, and 10 times the purified water, stir well, and then simmer at 50°C - 60°C over low heat to obtain wild jujube seed paste.

[0058] It should be noted that the decoction process is added after the secondary extraction. Two decoction processes are added. The two decoctions can fully release the components with strong heat resistance in the medicinal materials and enhance the overall efficacy of the medicine. In addition, the decoction process can further break the plant cell walls, promote the more complete dissolution of the active components in the medicinal materials, enhance the interaction between the components, and thus improve the overall efficacy of the medicinal paste.

[0059] Example 6 The preparation method of wild jujube seed paste is as follows: (1) Weigh the raw materials: Weigh 8 parts of wild jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese date, 0.8 part of γ-aminobutyric acid, 4 parts of Poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger by weight. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and 10 times the purified water into a vacuum concentration extraction tank. Set the negative pressure intensity in the container to 0.5 atm, soak at 25°C - 30°C in a sterile environment for 4 hours.

[0060] Next, continue with the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining a negative pressure intensity of 0.5 atm in the container: (2)Primary extraction: Extract the soaked raw materials, add 10 times the purified water by the weight of the raw materials, control the extraction temperature at 50°C, and the extraction time is 2 hours to obtain medicinal residues and an extract.

[0061] (3)Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank again, add 8 times the purified water by the weight of the medicinal residues, control the extraction temperature at 50°C as well, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts to obtain a combined extract.

[0062] (4)Decoction: Conduct the first decoction: Add the medicinal residues, purified water, and 4 parts of smoked plum (extra weighed raw materials) to the purified water, immerse the medicinal materials and residues in the water, bring to a boil, filter the decoction through a 500-mesh sieve, and then conduct the second decoction: Add 1 time the water to the decoction and continue to decoct, bring to a boil, filter the decoction through a 500-mesh sieve, combine the two decoctions and the previous combined extract to obtain a combined decoction.

[0063] (4)Concentration: Concentrate the combined decoction at 55°C using a vacuum concentration extraction tank to a relative density of 1.5 (measured at 25°C) of the clear paste to obtain the clear paste.

[0064] (5)Mix the prepared clear paste, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, and 10 times the purified water, stir well, and simmer at 50°C - 60°C over low heat to obtain wild jujube seed paste.

[0065] It should be noted that the decoction process is further optimized by adding smoked plum additionally to increase the extraction and transformation of active ingredients. By increasing the content of smoked plum, the enzymatic action of smoked plum is further enhanced, the transformation and release of components such as sapogenin and GABA in the medicinal materials are strengthened, and the medicinal effect is further improved. The organic acids and SOD in smoked plum can not only promote the conversion of jujube seed saponin A into jujube seed sapogenin, but also provide additional antioxidant and nerve-regulating effects, forming a stronger synergistic effect. Multiple active ingredients interact with each other during the decoction process, forming a stronger synergistic effect, making the paste formula more remarkable in terms of sedation, sleep aid, antioxidant, etc.

[0066] (II) Optimize the formula of the jujube seed paste Example 7 A method for preparing jujube seed paste, adding citric acid to the formula, and the specific preparation steps are as follows: (1) Weigh the raw materials: Weigh 8 parts of jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of smoked plum, 5 parts of mulberry, 8 parts of Chinese dates, 0.8 part of γ-aminobutyric acid, 4 parts of poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger by weight. Except for γ-aminobutyric acid and theanine, purify the other raw materials, and then pour the other raw materials and 10 times of purified water into a vacuum concentration extraction tank, set the negative pressure intensity in the container to 0.5 atm, soak at 25°C to 30°C in a sterile environment for 4 hours.

[0067] Next, continue with the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining the negative pressure intensity in the container at 0.5 atm: (2) Primary extraction: Extract the soaked raw materials, add 10 times the weight of the raw materials of purified water, add 4 parts of citric acid, adjust the pH of the solution to 4.0 - 4.5, and then start the extraction. The extraction temperature is controlled at 50°C, and the extraction time is 2 hours to obtain medicinal residues and an extract.

[0068] (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank again, add 8 times the weight of the medicinal residues of purified water, control the extraction temperature at 50°C as well, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts.

[0069] (4) Concentration: Under the condition of 50°C, further concentrate the combined extract using a vacuum concentration extraction tank until the relative density of the clear paste is 1.4 (measured at 30°C) to obtain a clear paste.

[0070] (5) Mix the prepared clear paste, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, and 10 times of purified water, stir well, and simmer at a low heat of 50°C - 60°C to obtain jujube seed paste.

[0071] It should be noted that by adding citric acid during the extraction stage and adjusting the pH value of the solution to 4.0 - 4.5, the hydrolysis of jujuboside A was effectively promoted. Jujuboside A is a complex compound containing glycosyl groups. The acidic environment can promote the hydrolysis of glycosyl groups, thereby generating more easily absorbable jujubogenin.

[0072] Adding citric acid not only enhanced the conversion rate of jujubogenin but also increased the dissolution rate of other active ingredients (such as polyphenols) by adjusting the pH environment, thus increasing the overall efficacy of the medicinal paste.

[0073] In addition, the addition of citric acid can not only adjust the acidity and alkalinity but also improve the taste of the medicinal paste, making it more acceptable to consumers, and inhibiting the growth of microorganisms through the acidic environment, thereby extending the shelf life of the medicinal paste. The jujube paste has a more pleasant taste in a moderately acidic environment, while improving the stability and storage time of the medicinal paste.

[0074] Example 8 A method for preparing jujube paste, adding various auxiliaries to the formula, and the specific preparation steps are as follows: (1) Weigh the raw materials: Weigh 8 parts of jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of dark plum, 5 parts of mulberry, 8 parts of Chinese date, 0.8 part of γ-aminobutyric acid, 4 parts of Poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and 10 times the purified water into a vacuum concentration extraction tank, set the negative pressure intensity in the container to 0.5 atm, soak at 25℃ - 30℃ in a sterile environment for 4 hours.

[0075] Next, continue with the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining the negative pressure intensity in the container at 0.5 atm: (2) Primary extraction: Extract the soaked raw materials, add 10 times the weight of the raw materials of purified water, control the extraction temperature at 85℃ - 90℃, and the extraction time is 2 hours to obtain medicinal residues and an extract.

[0076] (3) Secondary extraction: After the primary extraction, put the medicinal residues back into the extraction tank again, add 8 times the weight of the medicinal residues of purified water, control the extraction temperature at 85℃ - 90℃, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts.

[0077] (4) Concentration: Under the condition of 70℃ - 80℃, further concentrate the combined extract using a vacuum concentration extraction tank until the relative density of the clear paste is 1.2 (measured at 80℃) to obtain a clear paste.

[0078] (5) Mix the prepared clear extract, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, the auxiliary agent and 10 times of its purified water, stir well, and then boil it over low heat at 50°C - 60°C to obtain the wild jujube seed paste. Among them, at this time, the aforementioned auxiliary agent is 0.5 part of ergothioneine.

[0079] It should be noted that ergothioneine is a natural amino acid derivative with strong antioxidant effects, which can effectively neutralize free radicals and reduce cell damage. After introducing ergothioneine, the antioxidant performance of the medicinal extract has been significantly improved, which plays an important role in delaying aging, enhancing immunity, and promoting metabolism in the body.

[0080] In this application, it is found that 1) ergothioneine can cooperate with other components to enhance the antioxidant effect, protect active ingredients such as GABA and jujuboside A in the medicinal extract from oxidative degradation, and ensure the quality of the medicinal extract and the persistence of active ingredients. 2) Adding ergothioneine during boiling can not only improve the extraction efficiency, but also reduce the damage to active ingredients during boiling through the introduction of the auxiliary agent, ensuring the effectiveness and stability of the ingredients.

[0081] Example 9 A preparation method of wild jujube seed paste, adding a variety of auxiliary agents to the formula, and the specific preparation steps are as follows: (1) Weigh the raw materials: Weigh 8 parts of wild jujube seeds, 6 parts of longan pulp, 4 parts of raspberry, 4 parts of lotus seeds, 8 parts of lily, 4 parts of dark plum, 5 parts of mulberry, 8 parts of Chinese dates, 0.8 part of γ-aminobutyric acid, 4 parts of poria cocos, 0.6 part of theanine, 2 parts of fermented soybean, and 2 parts of dried ginger according to weight. Except for γ-aminobutyric acid and theanine, clean the other raw materials, and then pour the other raw materials and 10 times of purified water into the vacuum concentration extraction tank, set the negative pressure intensity in the container to 0.5 atm, soak at 25°C - 30°C in a sterile environment for 4 hours.

[0082] Next, continue with the primary extraction, secondary extraction, and concentration in the vacuum concentration extraction tank, maintaining the negative pressure intensity in the container at 0.5 atm: (2) Primary extraction: Extract the soaked raw materials, add 10 times the weight of purified water of the raw materials, control the extraction temperature at 85°C - 90°C, and the extraction time is 2 hours to obtain the medicinal residues and the extract.

[0083] (3) Secondary extraction: After the primary extraction, put the medicinal residues into the extraction tank again, add 8 times the weight of purified water of the medicinal residues, control the extraction temperature at 85°C - 90°C as well, and the extraction time is 2 hours; after the extraction is completed, combine the two extracts.

[0084] (4) Concentration: Under the condition of 70°C - 80°C, use the vacuum concentration extraction tank to further concentrate the combined extract until the relative density of the clear extract is 1.2 (measured at 80°C) to obtain the clear extract.

[0085] (5) Mix the prepared clear extract, 30 parts of refined honey, 0.8 part of γ-aminobutyric acid, 0.6 part of theanine, the auxiliary agent and 10 times of its purified water, stir well, and then boil it over low heat at 50°C - 60°C to obtain the wild jujube seed paste. Among them, at this time, the aforementioned auxiliary agent is 0.5 part of ergothioneine and 0.5 part of phycocyanin.

[0086] It should be noted that phycocyanin is a natural pigment protein extracted from blue-green algae and has various biological activities such as antioxidant, anti-inflammatory, and immunomodulatory effects. Phycocyanin can not only enhance the body's immunity but also improve the body's resistance to oxidative stress.

[0087] Ergothioneine and phycocyanin have different antioxidant mechanisms. The former is a powerful cytoprotectant that can protect cells by neutralizing free radicals and reducing oxidative damage, while phycocyanin supports the overall health of the body by enhancing immune function and anti-inflammation. After their synergistic effect, the antioxidant and immunomodulatory abilities of the medicinal plaster are further improved. The synergistic effect of the two auxiliary agents makes the efficacy of the medicinal plaster more comprehensive, especially outstanding in enhancing immunity, anti-inflammation, anti-fatigue, etc. In addition, they can also synergistically protect other active ingredients (such as GABA and jujuboside aglycone) in the medicinal plaster, improve the stability and efficacy of the medicinal plaster, and significantly increase the content of the active ingredients of the wild jujube seed paste.

[0088] In addition, a variety of auxiliary agents provide stronger protection for the thermosensitive components in the medicinal plaster, making the boiling process more stable and ensuring the medicinal efficacy of the medicinal plaster.

[0089] Further, it should be noted that in the above technical solution, the Chinese medicinal materials can be obtained from common Chinese medicinal material production companies in the Chinese market. In addition, the purchase methods of other reagents are provided: Citric acid: Pharmaceutical grade citric acid, 99% purity, Shanxi Jinyang Pharmaceutical Excipients Co., Ltd.

[0090] γ-aminobutyric acid: Food grade, 99% purity, Zhejiang Yingwodai Biotechnology Co., Ltd.

[0091] Theanine: Food grade, 20% purity, Xi'an Tianyi Biotechnology Co., Ltd.

[0092] Ergothioneine: Food grade, 99% purity, Shandong Yuanlitai Medical Technology Co., Ltd.

[0093] Phycocyanin: Phycocyanin E30, 100% purity, Zhejiang Binmei Biotechnology Co., Ltd.

[0094] Some Chinese medicinal materials can be purchased from Suzhou Tianling Chinese Medicinal Herb Slices Co., Ltd. The relevant batch numbers are provided below: Semen Ziziphi Spinosae: 20230816; Fructus Mori: 20230904; Arillus Longan: 20230829; Poria: 20230811; Bulbus Lilii: 20230830; Fructus Jujubae: 20230912; Semen Nelumbinis: 20230812; Fructus Mume: 20230808; Fructus Rubi: 20230810; Semen Sojae Praeparatum: 20230508; Rhizoma Zingiberis: 30230508.

[0095] (III) Detection 1. Detection of Jujubogenin (1) Weigh 1 g of the Semen Ziziphi Spinosae paste samples of Examples 1-10 and Comparative Example 1 respectively as samples.

[0096] Add the samples into 10 mL of 50% methanol solution (methanol-water, v / v 50:50), extract ultrasonically for 30 minutes, set the ultrasonic frequency to 40 kHz and the temperature to 30 °C. Centrifuge the extracted mixture at 4000 rpm for 10 minutes. Take the supernatant, filter it through a 0.22 μm filter membrane, collect the filtrate and prepare it for injection analysis.

[0097] (2) Liquid Chromatography Conditions Chromatographic column: C18 reversed-phase column (150 mm × 4.6 mm, 5 μm).

[0098] Mobile phase: Phase A is formic acid aqueous solution (containing 0.1% formic acid), and Phase B is acetonitrile.

[0099] Flow rate: 0.5 mL / min.

[0100] Column temperature: 30 °C.

[0101] Gradient elution program: 0-5 min: 5% Phase B 5-10 min: linearly increase 5% Phase B to 40% Phase B 10-20 min: linearly increase 40% Phase B to 90% Phase B 20-25 min: maintain 90% Phase B 25-30 min: linearly decrease 90% Phase B to 5% Phase B 30-35 min: maintain 5% Phase B to equilibrate the column Injection volume: 10 μL.

[0102] (3) Mass Spectrometry Conditions Mass spectrometry instrument: Triple Quadrupole MS.

[0103] Ionization mode: Electrospray ionization (ESI), with both positive ion mode (ESI+) and negative ion mode (ESI-) simultaneously.

[0104] 1) Jujubogenin (detected in negative ion mode, ESI-): Target ions: m / z 481.2 (parent ion), fragment ion m / z 319.1.

[0105] Collision energy: 25 eV.

[0106] Nebulizing gas flow rate: 10 L / min.

[0107] 2) γ-Aminobutyric acid (detected in positive ion mode, ESI+): Target ions: m / z 104.1 (parent ion), fragment ion m / z 87.1.

[0108] Collision energy: 10 eV.

[0109] Nebulizing gas flow rate: 10 L / min.

[0110] Ion source temperature: 300 °C.

[0111] (4) Recording data: Through the LC-MS / MS system, jujubogenin was detected in the negative ion mode and γ-aminobutyric acid was detected in the positive ion mode respectively. Record their respective retention times and the peak areas of the mass spectrometry fragment ions. Quantitative analysis was carried out using the standard curve method. Prepare standard solutions of jujubogenin and γ-aminobutyric acid with concentration ranges such as 10, 50, 100, 500, 1000 ng / mL, inject samples respectively to measure their peak areas, and plot the standard curve. According to the peak areas of jujubogenin and γ-aminobutyric acid in the sample, calculate their contents (mg / g) respectively using the standard curve.

[0112] (5) The results are as shown in Table 1 below: Table 1

[0113] In summary, this application deeply optimizes the traditional paste formula and its techniques of jujube seed paste from multiple aspects, including the improvement of the following technical means: 1. Negative pressure aseptic soaking: Before the first extraction, the medicinal materials and water are poured into the extraction tank, and soaked under a negative pressure intensity of 0.5 - 0.9 atm, at 25 °C - 30 °C, and in a sterile environment. For example, in Example 1.

[0114] 2. Optimize the medicinal material formula during negative pressure aseptic soaking. For example, in Example 3.

[0115] 3. Optimize the extraction process by adding primary extraction and secondary extraction and optimizing their parameters. For example, see Example 2.

[0116] 4. Optimize the medicinal material formula of the extraction process. For example, see Example 7.

[0117] 5. Optimize the medicinal material formula during the final decoction. For example, see Example 4, Example 8, and Example 9.

[0118] 6. Add a decoction process after the secondary extraction. For example, see Example 5.

[0119] 7. Optimize the medicinal material formula of the decoction process. For example, see Example 6.

[0120] This application combines technical means, examples, and comparative examples. By comparing between examples and between examples and comparative examples, it is proved that this application has successfully prepared a sour jujube paste and a preparation process using γ-aminobutyric acid and theanine as raw materials. Compared with the prior art, the various technical means of this application cooperate with each other, successfully and deeply optimize the traditional paste formula and its techniques of the sour jujube paste, and improve the content of active ingredients.

[0121] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the protection scope of the present invention. Any simple modification or equivalent replacement made by those of ordinary skill in the art to the technical solution of the present invention does not depart from the essence and scope of the technical solution of the present invention.

Claims

1. The jujube seed paste with γ-aminobutyric acid and tea theanine as raw materials is characterized by: The raw materials of the sour jujube kernel paste include sour jujube kernel, longan pulp, raspberry, lotus seed, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria, tea theanine, light black beans, dried ginger, and at least one of auxiliary agents; the auxiliary agent includes at least one of citric acid, ergothioneine, phycocyanin, and refined honey.

2. The Chinese date seed paste with γ-aminobutyric acid and tea theanine as raw materials according to claim 1, characterized in that: The raw materials of the sour jujube kernel paste include 5-60 parts of sour jujube kernel, 10-50 parts of longan pulp, 1-50 parts of raspberry, 1-50 parts of lotus seed, 3-50 parts of lily, 2-50 parts of black plum, 3-50 parts of mulberry, 3-50 parts of jujube, 0.1-10 parts of γ-aminobutyric acid, 0.1-10 parts of Poria cocos, 0.1-10 parts of tea theanine, 0.1-10 parts of light fermented black beans, 0.1-10 parts of dried ginger and 1-60 parts of at least one of the auxiliary agents.

3. The Chinese date seed paste with γ-aminobutyric acid and tea theanine as raw materials according to claim 1, characterized in that: By weight, the weight ratio of ergothioneine to phycocyanin is 1:0.5-2.

4. A method for preparing the Chinese date kernel paste using γ-aminobutyric acid and tea theanine as raw materials according to any one of claims 1 to 3, characterized in that: The steps include: (1) Weigh the raw materials: sour jujube seeds, longan pulp, raspberry, lotus seeds, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria, tea theanine, light black bean curd, and dried ginger. Except for γ-aminobutyric acid and tea theanine, clean the other raw materials, then pour the other raw materials and water into the extraction tank, set the negative pressure intensity of the container to 0.5~0.9 atm, 25℃~30℃, and soak in a sterile environment; (2) Primary extraction: extract the soaked raw materials, add water to extract, and obtain the residue and extract; (3) Secondary extraction: After the initial extraction, the drug residue is placed in the extraction tank again, water is added, and extraction is performed. After the extraction is completed, the two extracts are combined; (4) Concentration: The combined extract is further concentrated using an extraction tank to obtain a clear paste; (5) Mix the prepared clear paste, refined honey, γ-aminobutyric acid, tea theanine and water, stir well and simmer over low heat to obtain the sour jujube seed paste.

5. The method for preparing the Chinese date seed paste using γ-aminobutyric acid and tea theanine as raw materials according to claim 4, characterized in that: The steps include: (1) Weigh the raw materials: sour jujube seeds, longan pulp, raspberry, lotus seeds, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria, tea theanine, light black bean curd, and dried ginger. Except for γ-aminobutyric acid and tea theanine, clean the other raw materials, then pour the other raw materials and water into the extraction tank, set the negative pressure intensity of the container to 0.5~0.9 atm, 25℃~30℃, and soak in a sterile environment; (2) Primary extraction: extract the soaked raw materials by adding purified water 5 to 12 times the weight of the raw materials, control the extraction temperature at 50 to 60°C, and extract to obtain the residue and extract; (3) Secondary extraction: After the initial extraction, the drug residue is placed in the extraction tank again, and purified water 5 to 12 times the weight of the drug residue is added. The extraction temperature is also controlled at 50 to 60 °C, and the extraction is performed. After the extraction is completed, the two extracts are combined; (4) Concentration: The combined extracts are further concentrated in an extraction tank at 50-60°C until the relative density of the clear paste is 1.4-1.5, thereby obtaining a clear paste; (5) Mix the prepared clear paste, refined honey, γ-aminobutyric acid, tea theanine and water, stir well and simmer over low heat to obtain the sour jujube seed paste.

6. A method for preparing the Chinese date kernel paste using γ-aminobutyric acid and tea theanine as raw materials according to any one of claims 1 to 3, characterized in that: The steps include: (1) Weigh the raw materials: sour jujube seeds, longan pulp, raspberry, lotus seeds, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria cocos, tea theanine, light black bean curd, and dried ginger. Except for γ-aminobutyric acid and tea theanine, clean the other raw materials. Then pour all the raw materials including γ-aminobutyric acid and tea theanine and water into the extraction tank, set the negative pressure strength of the container to 0.5~0.9 atm, 25℃~30℃, and soak in a sterile environment; (2) Primary extraction: extract the soaked raw materials, add water to extract, and obtain the residue and extract; (3) Secondary extraction: After the initial extraction, the drug residue is placed in the extraction tank again, water is added, and extraction is performed. After the extraction is completed, the two extracts are combined; (4) Concentration: The combined extract is further concentrated using an extraction tank to obtain a clear paste; (5) Mix the prepared clear paste, refined honey and water, stir well and simmer over low heat to obtain the Chinese jujube kernel paste.

7. The method for preparing the Chinese date seed paste using γ-aminobutyric acid and tea theanine as raw materials according to claim 6, characterized in that: The steps include: (1) Weigh the raw materials: sour jujube seeds, longan pulp, raspberry, lotus seeds, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria cocos, tea theanine, light black bean curd, and dried ginger. Except for γ-aminobutyric acid and tea theanine, clean the other raw materials. Then pour all the raw materials including γ-aminobutyric acid and tea theanine and water into the extraction tank, set the negative pressure strength of the container to 0.5~0.9 atm, 25℃~30℃, and soak in a sterile environment; (2) Primary extraction: extract the soaked raw materials, add water to extract, and obtain the residue and extract; (3) Secondary extraction: After the initial extraction, the drug residue is placed in the extraction tank again, water is added, and extraction is performed. After the extraction is completed, the two extracts are combined; (4) Concentration: The combined extract is further concentrated using an extraction tank to obtain a clear paste; (5) Mix the prepared clear paste, refined honey, γ-aminobutyric acid, tea theanine and water, stir well and simmer over low heat to obtain the sour jujube seed paste.

8. A method for preparing the Chinese date seed paste using γ-aminobutyric acid and tea theanine as raw materials according to any one of claims 1 to 3, characterized in that: The steps include: (1) Weigh the raw materials: sour jujube seeds, longan pulp, raspberry, lotus seeds, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria, tea theanine, light black bean curd, and dried ginger. Except for γ-aminobutyric acid and tea theanine, the other raw materials are cleaned, and then the other raw materials and water are poured into the extraction tank, and the container is set to 0.5-0.9 atm negative pressure strength, 25°C-30°C, and soak in a sterile environment; (2) Primary extraction: extract the soaked raw materials, add water to extract, and obtain the residue and extract; (3) Secondary extraction: After the initial extraction, the drug residue is placed in the extraction tank again, water is added, and extraction is performed. After the extraction is completed, the two extracts are combined to obtain a combined extract; (4) decoction: performing a first decoction: adding the medicinal residue to water and / or black plums for decoction, filtering the decoction through a sieve, and then performing a second decoction: adding water to the decoction and continuing to decoct, filtering the decoction through a sieve, combining the two decoctions and the combined extract to obtain a combined decoction; (5) Concentration: The combined decoction is further concentrated using an extraction tank to obtain a clear paste; (6) Mix the prepared clear paste, refined honey, γ-aminobutyric acid, tea theanine and water, stir well and simmer over low heat to obtain the sour jujube seed paste.

9. A method for preparing the Chinese date kernel paste using γ-aminobutyric acid and tea theanine as raw materials according to any one of claims 1 to 3, characterized in that: The steps include: (1) Weigh the raw materials: sour jujube seeds, longan pulp, raspberry, lotus seeds, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria, tea theanine, light black bean curd, and dried ginger. Except for γ-aminobutyric acid and tea theanine, clean the other raw materials, then pour the other raw materials and water into the extraction tank, set the negative pressure intensity of the container to 0.5~0.9 atm, 25℃~30℃, and soak in a sterile environment; (2) Primary extraction: extract the soaked raw material, add water, then add citric acid, adjust the pH of the solution to 4.0-4.5, extract, and obtain the residue and extract; (3) Secondary extraction: After the initial extraction, the drug residue is placed in the extraction tank again, water is added, and extraction is performed. After the extraction is completed, the two extracts are combined; (4) Concentration: The combined extract is further concentrated using an extraction tank to obtain a clear paste; (5) Mix the prepared clear paste, refined honey, γ-aminobutyric acid, tea theanine and water, stir well and simmer over low heat to obtain the sour jujube seed paste.

10. A method for preparing the Chinese date seed paste using γ-aminobutyric acid and tea theanine as raw materials according to any one of claims 1 to 3, characterized in that: The steps include: (1) Weigh the raw materials: sour jujube seeds, longan pulp, raspberry, lotus seeds, lily, black plum, mulberry, jujube, γ-aminobutyric acid, Poria, tea theanine, light black bean curd, and dried ginger. Except for γ-aminobutyric acid and tea theanine, clean the other raw materials, then pour the other raw materials and water into the extraction tank, set the negative pressure intensity of the container to 0.5~0.9 atm, 25℃~30℃, and soak in a sterile environment; (2) Primary extraction: extract the soaked raw materials, add water to extract, and obtain the residue and extract; (3) Secondary extraction: After the initial extraction, the drug residue is placed in the extraction tank again, water is added, and extraction is performed. After the extraction is completed, the two extracts are combined; (4) Concentration: The combined extract is further concentrated using an extraction tank to obtain a clear paste; (5) The prepared clear paste, refined honey, γ-aminobutyric acid, tea theanine, water and / or ergothioneine and / or phycocyanin are mixed, stirred evenly and simmered over low heat to obtain the jujube seed paste.

Images