Method for producing organic fertilizer by utilizing lincomycin mushroom dregs and organic fertilizer thereof

Abstract

The invention relates to the field of fertilizers, in particular to a method for producing an organic fertilizer from lincomycin residues and the organic fertilizer thereof.The method comprises the following steps that 1, fresh lincomycin residues are placed in a drying oven to be dried, ground and sieved, and lincomycin residue powder is obtained; 2, treating by using an electron beam irradiation technology to obtain irradiated lincomycin mushroom dreg powder; step 3, preparing sulfonated alkali lignin; step 4, mixing the irradiated lincomycin mushroom dreg powder and sulfonated alkali lignin, and heating for reaction to obtain alkali lignin modified lincomycin mushroom dreg; and step 5, mixing the alkali lignin modified lincomycin residues with livestock and poultry manure, scattering aerobic bacteria, fully stirring, and carrying out aerobic composting treatment to obtain the organic fertilizer. The organic fertilizer prepared by the invention has the advantages of low antibiotic content and high nutrient content, can promote green growth, flowering and fruiting of plants, and improves the stress resistance at the same time.

Description

Technical Field

[0001] The present invention relates to the field of fertilizers, and particularly relates to a method for producing organic fertilizers by using lincomycin mycelium residue and the organic fertilizers produced thereby. Background Art

[0002] Antibiotic mycelium residue is solid waste generated during the production of antibiotics. Its main components include the mycelium of antibiotic-producing bacteria, unused culture medium, metabolites produced during fermentation, degradation products of the culture medium, and a small amount of antibiotics, etc. Existing antibiotic mycelium residues mainly include tetracyclines, aminoglycosides, polypeptides, macrolides, antifungal agents, antituberculosis agents, etc. Because they contain quite a lot of nutrients, in the past, the treatment method of antibiotic mycelium residue was to be dried and used as feed or feed additive. Due to the residual small amount of antibiotics in it, if animals eat it for a long time, it will cause problems such as drug resistance and zoonoses. In 2002, it was included in the "List of Drug Varieties Prohibited from Use in Feed and Drinking Water for Animals".

[0003] Lincomycin belongs to lincomycin antibiotics and has good thermal stability. It can stably exist at 70 °C for 1 month. At present, ozone oxidation, titanium dioxide photocatalytic oxidation, cow dung composting and other methods are used at home and abroad to remove the small amount of lincomycin remaining in the mycelium residue. However, the application of these methods is limited and it has an impact on the nutrient components in the mycelium residue. At present, the method adopted by each pharmaceutical enterprise to treat lincomycin mycelium residue is high-temperature incineration at 500 - 600 °C. Incineration not only brings a huge economic burden to the pharmaceutical enterprise, but also the useful components in the mycelium residue are not utilized, resulting in waste of resources. Anaerobic digestion treatment is a complex ecological system. The raw material ratio has a great influence on the anaerobic digestion effect. Its antibiotic removal rate is high, but the amount of pollutants generated is large, and the treatment of wastewater and waste residue is difficult. The aerobic composting method can enable the residual antibiotics in the mycelium residue to be utilized, decomposed or transformed into other harmless substances by aerobic microorganisms, realizing the harmless treatment of the mycelium residue, and developing special fertilizers to realize the resource utilization of the mycelium residue. Although this method has good degradation effect, it has not been popularized to actual application. Therefore, the application of this method in practice still has limitations, with a long time and difficult to control. Moreover, the existing aerobic composting technology has not completely degraded the lincomycin in the mycelium residue, and there is still a small amount of antibiotic residue, which has a certain impact on the environment. Summary of the Invention

[0004] Aiming at the problems existing in the prior art, the purpose of the present invention is to provide a method for producing organic fertilizers by using lincomycin mycelium residue and the organic fertilizers produced thereby.

[0005] The purpose of the present invention is achieved by adopting the following technical solutions: In the first aspect, the present invention provides a method for producing organic fertilizers by using lincomycin mycelium residue, including the following steps: Step 1: Place the fresh lincomycin bacterial residue in an oven to dry, then place it in a mortar to grind, and then sieve it. Collect the powder to obtain lincomycin bacterial residue powder. Step 2: Spread out the lincomycin bacterial residue powder and use electron beam irradiation technology for treatment to obtain irradiated lincomycin bacterial residue powder. Step 3: Mix alkali lignin and sodium hydroxide solution, heat up to 50 - 80 °C, stir well for 15 - 45 min, then add formaldehyde solution, keep stirring for 1 - 2 h while maintaining the temperature, add sodium sulfite, heat up to 80 - 100 °C, keep stirring for 2 - 4 h. After the treatment, cool down to room temperature and discharge the product in absolute ethanol to obtain sulfonated alkali lignin. Step 4: Mix the irradiated lincomycin bacterial residue powder and sulfonated alkali lignin in an organic solvent, then add a catalyst, stir well, heat up for reaction. After the reaction is completed, remove the solvent, wash three times with alcohol and then dry to obtain alkali lignin - modified lincomycin bacterial residue. Step 5: Mix the alkali lignin - modified lincomycin bacterial residue and livestock manure, then sprinkle aerobic bacteria, stir well, and conduct aerobic composting treatment to obtain organic fertilizer.

[0006] Preferably, in Step 1, the content of lincomycin in the fresh lincomycin bacterial residue is 0.43 wt%.

[0007] Preferably, in Step 1, the temperature in the oven is 110 - 150 °C, and dry until the water content is less than 10%.

[0008] Preferably, in Step 1, the grinding speed is 200 - 300 r / min, and sieve through a 100 - mesh sieve after grinding.

[0009] Preferably, in Step 2, the parameters of the electron beam irradiation technology include: the accelerator energy is 1.3 - 2 MeV, the beam current is 30 - 80 mA; the irradiation absorption dose is 50 - 100 kGy.

[0010] Preferably, in Step 3, the mass fraction of the sodium hydroxide solution is 1% - 5%, and the mass fraction of the formaldehyde solution is 35% - 40%.

[0011] Preferably, in Step 3, the mass ratio of alkali lignin, formaldehyde, sodium sulfite and sodium hydroxide solution is 1:0.5 - 0.8:0.2 - 0.4:5 - 10.

[0012] Preferably, in Step 4, the mass ratio of the irradiated lincomycin bacterial residue powder, sulfonated alkali lignin and organic solvent is 1:0.2 - 0.7:15 - 25.

[0013] Preferably, in the step 4, the catalyst is phosphotungstic acid, and the addition amount is 2%-7% of the mass of sulfonated alkali lignin; the reaction temperature is 90-130 °C, and keep stirring for 2-5 h.

[0014] Preferably, in the step 5, the aerobic bacteria are Pseudomonas RST-1 in the genus Pseudomonas, and the effective viable count is ≥ 2×10 9 cfu / g.

[0015] Preferably, in the step 5, the addition amount of the aerobic bacteria is 1%-3.5% of the mass of alkali lignin-modified lincomycin residue.

[0016] Preferably, in the step 5, the mass ratio of irradiated lincomycin residue powder to sulfonated alkali lignin is 1:1-3.

[0017] Preferably, in the step 5, during the composting process, the aeration rate is 0.1-1 L / min / kg, turning the pile once every 12 h, and composting for 5-10 days.

[0018] In the second aspect, the present invention provides an organic fertilizer produced by using lincomycin residue, which is prepared by the above preparation method.

[0019] The beneficial effects of the present invention are as follows: 1. The present invention provides a method for producing organic fertilizer by using lincomycin residue, including irradiating fresh lincomycin residue with electron beam to eliminate most of the lincomycin therein, then performing modification treatment to obtain alkali lignin-modified lincomycin residue, and finally mixing it with livestock and poultry manure for aerobic composting to finally prepare organic fertilizer. The organic fertilizer prepared by the present invention has the advantages of low antibiotics and high nutrients, can promote the green growth, flowering and fruiting of plants, and at the same time increase the stress resistance.

[0020] 2. The modification process of the lincomycin residue after irradiation treatment in the present invention includes: first preparing sulfonated alkali lignin, and then using sulfonated alkali lignin to react with lincomycin residue to prepare alkali lignin-modified lincomycin residue. The residue after modification not only has enhanced adsorption, but also the nutrients therein are enhanced, with a certain slow-release effect, and can form a highly efficient slow-release organic fertilizer during the subsequent composting process, which has a good promoting effect on the growth of plants. Specific Embodiments

[0021] The technical solution of the present invention will be described below through specific specific examples. It should be understood that one or more method steps mentioned in the present invention do not exclude the existence of other method steps before and after the combined steps or the insertion of other method steps between these clearly mentioned steps; it should also be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. Moreover, unless otherwise specified, the numbers of the method steps are only convenient tools for identifying the method steps, rather than limiting the arrangement order of the method steps or the scope in which the present invention can be implemented. The change or adjustment of their relative relationship, without substantial change in the technical content, should also be regarded as the scope in which the present invention can be implemented.

[0022] To better understand the above technical solution, the exemplary embodiments of the present invention will be described in more detail below. Although the exemplary embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0023] The present invention will be further described below in conjunction with the following embodiments.

[0024] Embodiment 1 A method for producing organic fertilizer using lincomycin bacterial residue, comprising the following steps: Step 1: Place fresh lincomycin bacterial residue (the content of lincomycin is 0.43 wt%) in an oven, the temperature in the oven is 120 °C, dry it until the water content is lower than 10%, then place it in a mortar and grind it at a speed of 250 r / min, and then pass through a 100-mesh sieve to collect the powder to obtain lincomycin bacterial residue powder; Step 2: Spread out the lincomycin bacterial residue powder and use electron beam irradiation technology for treatment. The accelerator energy is 1.8 MeV and the beam current is 50 mA; the irradiation absorption dose is 80 kGy to obtain irradiated lincomycin bacterial residue powder; Step 3: Mix alkali lignin and a sodium hydroxide solution with a mass fraction of 2%, heat it to 60 °C, fully stir for 30 min, then add a formaldehyde solution with a mass fraction of 38%, keep stirring for 1.5 h after insulation, add sodium sulfite, and the mass ratio of alkali lignin, formaldehyde, sodium sulfite and sodium hydroxide solution is 1:0.6:0.3:8. Heat it to 90 °C and keep stirring for 3 h. After the treatment is completed, cool it to room temperature and discharge it in absolute ethanol to obtain sulfonated alkali lignin; Step 4, mixing the irradiated lincomycin residue powder and the sulfonated alkali lignin in an organic solvent, wherein the mass ratio of the irradiated lincomycin residue powder, the sulfonated alkali lignin and the organic solvent is 1:0.4:20, and then adding a catalyst phosphotungstic acid in an amount of 5% of the mass of the sulfonated alkali lignin, stirring thoroughly, heating to react at a reaction temperature of 110° C., and stirring for 3 hours at the same temperature. After the reaction is completed, removing the solvent, washing with alcohol three times, and drying to obtain alkali lignin-modified lincomycin residue; Step 5: mix the alkali lignin modified lincomycin residue and livestock manure in a mass ratio of 1:2, and then sprinkle Pseudomonas RST-1 from the genus Pseudomonas, with an effective live bacterial count of ≥2×10 9 cfu / g, the amount of aerobic bacteria added is 2.5% of the mass of the modified lincomycin residue. After sufficient stirring, aerobic composting is carried out with an aeration rate of 0.5L / min / kg. The compost is turned every 12 hours. The composting is carried out for 7 days to obtain organic fertilizer.

[0025] Example 2 A method for producing organic fertilizer using lincomycin residue comprises the following steps: Step 1, placing fresh lincomycin residue (lincomycin content of 0.43wt%) in an oven at a temperature of 110°C, drying until the moisture content is less than 10%, then grinding in a mortar at a speed of 200r / min, and then passing through a 100-mesh sieve to collect powder to obtain lincomycin residue powder; Step 2, spreading the lincomycin residue powder, and treating it using electron beam irradiation technology, with an accelerator energy of 1.3 MeV and a beam current of 30 mA; the irradiation absorbed dose is 50 kGy, to obtain irradiated lincomycin residue powder; Step 3, mixing alkali lignin and a sodium hydroxide solution with a mass fraction of 1%, heating to 50-80°C, stirring for 15 minutes, adding a formaldehyde solution with a mass fraction of 35%, keeping warm and stirring for 1 hour, adding sodium sulfite, the mass ratio of alkali lignin, formaldehyde, sodium sulfite and sodium hydroxide solution is 1:0.5:0.2:5, heating to 80°C, keeping warm and stirring for 2 hours, cooling to room temperature after the treatment, and discharging in anhydrous ethanol to obtain sulfonated alkali lignin; Step 4, mixing the irradiated lincomycin residue powder and the sulfonated alkali lignin in an organic solvent, wherein the mass ratio of the irradiated lincomycin residue powder, the sulfonated alkali lignin and the organic solvent is 1:0.2:15, and then adding a catalyst phosphotungstic acid in an amount of 2% of the mass of the sulfonated alkali lignin, stirring thoroughly, heating to react at 90° C., and stirring for 2 hours. After the reaction is completed, removing the solvent, washing with alcohol three times, and drying to obtain alkali lignin-modified lincomycin residue; Step 5: mix the alkali lignin modified lincomycin residue and livestock manure in a mass ratio of 1:3, and then sprinkle Pseudomonas RST-1 from the genus Pseudomonas, with an effective live bacterial count of ≥2×10 9 cfu / g, the amount of aerobic bacteria added is 1% of the mass of the modified lincomycin residue. After sufficient stirring, aerobic composting is carried out with an aeration rate of 0.1L / min / kg. The pile is turned every 12 hours and composting is carried out for 5 days to obtain organic fertilizer.

[0026] Example 3 A method for producing organic fertilizer using lincomycin residue comprises the following steps: Step 1, placing fresh lincomycin residue (lincomycin content of 0.43wt%) in an oven at a temperature of 150°C, drying until the moisture content is less than 10%, then grinding in a mortar at a speed of 300r / min, and then passing through a 100-mesh sieve to collect powder to obtain lincomycin residue powder; Step 2, spreading the lincomycin residue powder, and treating it using electron beam irradiation technology, with an accelerator energy of 2 MeV and a beam current of 80 mA; the irradiation absorbed dose is 100 kGy, to obtain irradiated lincomycin residue powder; Step 3, mixing alkali lignin and a 5% sodium hydroxide solution by mass, heating to 80° C., stirring for 45 minutes, adding a 40% formaldehyde solution by mass, keeping warm and stirring for 2 hours, adding sodium sulfite, the mass ratio of alkali lignin, formaldehyde, sodium sulfite and sodium hydroxide solution is 1:0.8:0.4:10, heating to 100° C., keeping warm and stirring for 4 hours, cooling to room temperature after the treatment, and discharging in anhydrous ethanol to obtain sulfonated alkali lignin; Step 4, mixing the irradiated lincomycin residue powder and the sulfonated alkali lignin in an organic solvent, wherein the mass ratio of the irradiated lincomycin residue powder, the sulfonated alkali lignin and the organic solvent is 1:0.7:25, and then adding a catalyst phosphotungstic acid in an amount of 7% of the mass of the sulfonated alkali lignin, stirring thoroughly, heating to react at a reaction temperature of 130° C., and stirring for 5 hours at the temperature. After the reaction is completed, removing the solvent, washing with alcohol three times, and drying to obtain alkali lignin-modified lincomycin residue; Step 5: mix the alkali lignin modified lincomycin residue and livestock manure in a mass ratio of 1:1, and then sprinkle Pseudomonas RST-1 of the genus Pseudomonas, with an effective live bacterial count of ≥2×10 9 cfu / g, the amount of aerobic bacteria added is 3.5% of the mass of the modified lincomycin residue. After sufficient stirring, aerobic composting is carried out with an aeration rate of 1L / min / kg. The pile is turned every 12 hours and the composting is carried out for 10 days to obtain organic fertilizer.

[0027] Example 4 A method for producing organic fertilizer from lincomycin mycelial residue, comprising the following steps: Step 1: Place fresh lincomycin mycelial residue (with a lincomycin content of 0.43 wt%) in an oven at a temperature of 120 °C and dry it until the water content is less than 10%. Then place it in a mortar and grind it at a speed of 210 r / min. Then pass it through a 100-mesh sieve and collect the powder to obtain lincomycin mycelial residue powder; Step 2: Spread out the lincomycin mycelial residue powder and treat it using electron beam irradiation technology. The accelerator energy is 1.4 MeV and the beam current is 40 mA; the irradiation absorption dose is 60 kGy to obtain irradiated lincomycin mycelial residue powder; Step 3: Mix alkali lignin with a 2% sodium hydroxide solution, heat it to 60 °C, stir well for 25 min, then add a 36% formaldehyde solution, keep stirring for 1 - 2 h, add sodium sulfite. The mass ratio of alkali lignin, formaldehyde, sodium sulfite, and sodium hydroxide solution is 1:0.6:0.3:8. Heat it to 80 °C and keep stirring for 2 h. After the treatment, cool it to room temperature and discharge it in absolute ethanol to obtain sulfonated alkali lignin; Step 4: Mix the irradiated lincomycin mycelial residue powder and sulfonated alkali lignin in an organic solvent. The mass ratio of irradiated lincomycin mycelial residue powder, sulfonated alkali lignin, and organic solvent is 1:0.3:20. Then add the catalyst phosphotungstic acid, and the addition amount is 3% of the mass of sulfonated alkali lignin. After stirring well, heat it for reaction. The reaction temperature is 100 °C, keep stirring for 3 h. After the reaction is completed, remove the solvent, wash it with alcohol three times and then dry it to obtain alkali lignin-modified lincomycin mycelial residue; Step 5: Mix the alkali lignin-modified lincomycin mycelial residue and livestock and poultry manure, and then sprinkle Pseudomonas RST-1 in the aerobic bacterium Pseudomonas. The effective viable count is ≥2×10 9 cfu / g, and the addition amount of the aerobic bacterium is 2% of the mass of the modified lincomycin mycelial residue. After stirring well, perform aerobic composting treatment. The aeration rate is 0.2 L / min / kg, turn the pile once every 12 h, and perform composting treatment for 7 days to obtain organic fertilizer.

[0028] Example 5 A method for producing organic fertilizer from lincomycin mycelial residue, comprising the following steps: Step 1: Place fresh lincomycin mycelial residue (with a lincomycin content of 0.43 wt%) in an oven at a temperature of 125 °C and dry it until the water content is less than 10%. Then place it in a mortar and grind it at a speed of 250 r / min. Then pass it through a 100-mesh sieve and collect the powder to obtain lincomycin mycelial residue powder; Step 2: Spread out the lincomycin residue powder and treat it using electron beam irradiation technology. The accelerator energy is 1.5 MeV, the beam current is 50 mA, and the irradiation absorbed dose is 70 kGy to obtain irradiated lincomycin residue powder. Step 3: Mix alkali lignin with a 2% sodium hydroxide solution by mass, heat up to 70 °C, stir well for 30 min, then add a 35% formaldehyde solution by mass, keep stirring for 2 h while maintaining the temperature, add sodium sulfite. The mass ratio of alkali lignin, formaldehyde, sodium sulfite, and sodium hydroxide solution is 1:0.6:0.3:10. Heat up to 100 °C, keep stirring for 3 h. After the treatment is completed, cool down to room temperature and discharge the product in absolute ethanol to obtain sulfonated alkali lignin. Step 4: Mix the irradiated lincomycin residue powder and sulfonated alkali lignin in an organic solvent. The mass ratio of irradiated lincomycin residue powder, sulfonated alkali lignin, and organic solvent is 1:0.3:25. Then add the catalyst phosphotungstic acid, and the addition amount is 4% of the mass of sulfonated alkali lignin. After stirring well, heat up for reaction. The reaction temperature is 100 °C, keep stirring for 4 h. After the reaction is completed, remove the solvent, wash three times with alcohol and then dry to obtain alkali lignin-modified lincomycin residue. Step 5: Mix the alkali lignin-modified lincomycin residue and livestock and poultry manure, and then sprinkle Pseudomonas RST-1 in the aerobic bacterium Pseudomonas. The effective viable count is ≥2×10 9 cfu / g, and the addition amount of the aerobic bacterium is 2.5% of the mass of the modified lincomycin residue. After stirring well, perform aerobic composting treatment. The aeration rate is 0.3 L / min / kg, turn the pile once every 12 h, and perform composting treatment for 5 days to obtain organic fertilizer.

[0029] Example 6 A method for producing organic fertilizer using lincomycin residue, comprising the following steps: Step 1: Place fresh lincomycin residue (the content of lincomycin is 0.43 wt%) in an oven. The temperature in the oven is 125 °C, dry it until the water content is less than 10%, then place it in a mortar and grind it at a speed of 200 r / min, and then pass through a 100-mesh sieve to collect the powder to obtain lincomycin residue powder. Step 2: Spread out the lincomycin residue powder and treat it using electron beam irradiation technology. The accelerator energy is 1.3 MeV, the beam current is 30 mA, and the irradiation absorbed dose is 100 kGy to obtain irradiated lincomycin residue powder. Step 3: Mix alkali lignin with a 3% sodium hydroxide solution, heat up to 65°C, stir well for 40 min, then add a 35% formaldehyde solution. After stirring for 1 h while maintaining the temperature, add sodium sulfite. The mass ratio of alkali lignin, formaldehyde, sodium sulfite, and sodium hydroxide solution is 1:0.6:0.2:10. Heat up to 80°C and stir for 4 h while maintaining the temperature. After the treatment, cool down to room temperature and discharge the product in absolute ethanol to obtain sulfonated alkali lignin. Step 4: Mix irradiated lincomycin residue powder and sulfonated alkali lignin in an organic solvent. The mass ratio of irradiated lincomycin residue powder, sulfonated alkali lignin, and organic solvent is 1:0.2:25. Then add the catalyst phosphotungstic acid, and the addition amount is 2% of the mass of sulfonated alkali lignin. After stirring well, heat up for reaction. The reaction temperature is 130°C, stir for 2 h while maintaining the temperature. After the reaction is completed, remove the solvent, wash three times with alcohol, and then dry to obtain alkali lignin-modified lincomycin residue. Step 5: Mix alkali lignin-modified lincomycin residue and livestock manure, and then sprinkle Pseudomonas RST-1 in the aerobic bacterium Pseudomonas. The effective viable count is ≥2×10 9 cfu / g, and the addition amount of the aerobic bacterium is 3.5% of the mass of modified lincomycin residue. After stirring well, perform aerobic composting treatment. The aeration rate is 0.3 L / min / kg, turn the pile every 12 h, and carry out composting treatment for 10 days to obtain organic fertilizer.

[0030] Example 7 A method for producing organic fertilizer using lincomycin residue, comprising the following steps: Step 1: Place fresh lincomycin residue (with a lincomycin content of 0.43 wt%) in an oven. The temperature in the oven is 130°C. Dry it until the water content is less than 10%, then place it in a mortar and grind at a speed of 300 r / min, and then pass through a 100-mesh sieve to collect the powder to obtain lincomycin residue powder. Step 2: Spread out the lincomycin residue powder and use electron beam irradiation technology for treatment. The accelerator energy is 1.7 MeV, the beam current is 65 mA, and the irradiation absorption dose is 70 kGy to obtain irradiated lincomycin residue powder. Step 3: Mix alkali lignin with a 2% sodium hydroxide solution, heat up to 80°C, stir well for 35 min, then add a 35% formaldehyde solution. After stirring for 2 h while maintaining the temperature, add sodium sulfite. The mass ratio of alkali lignin, formaldehyde, sodium sulfite, and sodium hydroxide solution is 1:0.6:0.3:10. Heat up to 100°C and stir for 2 h while maintaining the temperature. After the treatment, cool down to room temperature and discharge the product in absolute ethanol to obtain sulfonated alkali lignin. Step 4, mixing the irradiated lincomycin residue powder and the sulfonated alkali lignin in an organic solvent, wherein the mass ratio of the irradiated lincomycin residue powder, the sulfonated alkali lignin and the organic solvent is 1:0.5:15, and then adding a catalyst phosphotungstic acid in an amount of 2% of the mass of the sulfonated alkali lignin, stirring thoroughly, heating to react at a reaction temperature of 130° C., and stirring for 2 hours at the same temperature. After the reaction is completed, removing the solvent, washing three times with alcohol, and drying to obtain alkali lignin-modified lincomycin residue; Step 5: Mix the alkali lignin modified lincomycin residue with livestock manure, and then sprinkle Pseudomonas RST-1 from the genus Pseudomonas, with the effective live bacteria count ≥ 2×10 9 cfu / g, the amount of aerobic bacteria added is 3.5% of the mass of the modified lincomycin residue. After sufficient stirring, aerobic composting is carried out with an aeration rate of 0.1L / min / kg. The pile is turned every 12 hours. The composting is carried out for 10 days to obtain organic fertilizer.

[0031] Example 8 A method for producing organic fertilizer using lincomycin residue comprises the following steps: Step 1, placing fresh lincomycin residue (lincomycin content of 0.43wt%) in an oven at a temperature of 140°C, drying until the moisture content is less than 10%, then grinding in a mortar at a grinding speed of 230r / min, and then passing through a 100-mesh sieve to collect powder to obtain lincomycin residue powder; Step 2, spreading the lincomycin residue powder, and treating it using electron beam irradiation technology, with an accelerator energy of 1.4 MeV and a beam current of 50 mA; the irradiation absorbed dose is 90 kGy, to obtain irradiated lincomycin residue powder; Step 3, mixing alkali lignin and a 3% sodium hydroxide solution by mass, heating to 60° C., stirring for 25 minutes, adding a 35% formaldehyde solution by mass, keeping warm and stirring for 2 hours, adding sodium sulfite, the mass ratio of alkali lignin, formaldehyde, sodium sulfite and sodium hydroxide solution is 1:0.7:0.3:10, heating to 80° C., keeping warm and stirring for 4 hours, cooling to room temperature after the treatment, and discharging in anhydrous ethanol to obtain sulfonated alkali lignin; Step 4, mixing the irradiated lincomycin residue powder and the sulfonated alkali lignin in an organic solvent, wherein the mass ratio of the irradiated lincomycin residue powder, the sulfonated alkali lignin and the organic solvent is 1:0.4:20, and then adding a catalyst phosphotungstic acid in an amount of 3% of the mass of the sulfonated alkali lignin, stirring thoroughly, heating to react at a reaction temperature of 100° C., and stirring for 3 hours at the same temperature. After the reaction is completed, removing the solvent, washing with alcohol three times, and then drying to obtain alkali lignin-modified lincomycin residue; Step 5: Mix the alkali lignin-modified lincomycin residue and livestock manure, and then sprinkle Pseudomonas RST-1 of the aerobic bacterium Pseudomonas genus into it, with the effective viable count ≥ 2×10 9 cfu / g. The addition amount of the aerobic bacterium is 1.5% of the mass of the modified lincomycin residue. After sufficient stirring, perform aerobic composting treatment with an aeration rate of 0.5 L / min / kg, turn the pile every 12 h, and conduct composting treatment for 5 days to obtain organic fertilizer.

[0032] Example 9 A method for producing organic fertilizer using lincomycin residue, comprising the following steps: Step 1: Place fresh lincomycin residue (with a lincomycin content of 0.43 wt%) in an oven at a temperature of 125 °C and dry it until the water content is lower than 10%. Then place it in a mortar and grind it at a speed of 270 r / min, and then pass it through a 100-mesh sieve to collect the powder to obtain lincomycin residue powder; Step 2: Spread out the lincomycin residue powder and use electron beam irradiation technology for treatment. The accelerator energy is 1.6 MeV and the beam current is 40 mA; the irradiation absorption dose is 70 kGy to obtain irradiated lincomycin residue powder; Step 3: Mix alkali lignin and a 4% sodium hydroxide solution, heat it to 70 °C, stir it sufficiently for 35 min, then add a 35% formaldehyde solution, keep stirring for 2 h after insulation, add sodium sulfite, and the mass ratio of alkali lignin, formaldehyde, sodium sulfite, and sodium hydroxide solution is 1:0.7:0.3:10. Heat it to 80 °C and keep stirring for 4 h. After the treatment is completed, cool it to room temperature and discharge it in absolute ethanol to obtain sulfonated alkali lignin; Step 4: Mix the irradiated lincomycin residue powder and sulfonated alkali lignin in an organic solvent. The mass ratio of the irradiated lincomycin residue powder, sulfonated alkali lignin, and organic solvent is 1:0.6:20. Then add the catalyst phosphotungstic acid, and the addition amount is 5% of the mass of sulfonated alkali lignin. After sufficient stirring, heat up the reaction. The reaction temperature is 120 °C, keep stirring for 4 h. After the reaction is completed, remove the solvent, wash it three times with alcohol and then dry it to obtain alkali lignin-modified lincomycin residue; Step 5: Mix the alkali lignin-modified lincomycin residue and livestock manure, and then sprinkle Pseudomonas RST-1 of the aerobic bacterium Pseudomonas genus into it, with the effective viable count ≥ 2×10 9 cfu / g. The addition amount of the aerobic bacterium is 3% of the mass of the modified lincomycin residue. After sufficient stirring, perform aerobic composting treatment with an aeration rate of 0.7 L / min / kg, turn the pile every 12 h, and conduct composting treatment for 5 - 10 days to obtain organic fertilizer.

[0033] Example 10 A method for producing organic fertilizer using lincomycin mycelial residue, comprising the following steps: Step 1: Place fresh lincomycin mycelial residue (with a lincomycin content of 0.43 wt%) in an oven. The temperature in the oven is 150 °C. Dry it until the water content is lower than 10%. Then place it in a mortar and grind it at a speed of 200 r / min. Then pass it through a 100-mesh sieve and collect the powder to obtain lincomycin mycelial residue powder; Step 2: Spread out the lincomycin mycelial residue powder and use electron beam irradiation technology for treatment. The accelerator energy is 2 MeV and the beam current is 80 mA; the irradiation absorption dose is 100 kGy to obtain irradiated lincomycin mycelial residue powder; Step 3: Mix alkali lignin and a 1% sodium hydroxide solution, heat it to 50 °C, stir well for 45 min, then add a 35%-40% formaldehyde solution, keep stirring for 1 h after insulation, add sodium sulfite. The mass ratio of alkali lignin, formaldehyde, sodium sulfite and sodium hydroxide solution is 1:0.5:0.4:5. Heat it to 100 °C and keep stirring for 2 h. After the treatment is completed, cool it to room temperature and discharge it in absolute ethanol to obtain sulfonated alkali lignin; Step 4: Mix the irradiated lincomycin mycelial residue powder and sulfonated alkali lignin in an organic solvent. The mass ratio of the irradiated lincomycin mycelial residue powder, sulfonated alkali lignin and organic solvent is 1:0.2:25. Then add the catalyst phosphotungstic acid, and the addition amount is 7% of the mass of sulfonated alkali lignin. After stirring well, heat up for reaction. The reaction temperature is 130 °C, keep stirring for 2 h. After the reaction is completed, remove the solvent, wash it three times with alcohol and then dry it to obtain alkali lignin-modified lincomycin mycelial residue; Step 5: Mix the alkali lignin-modified lincomycin mycelial residue and livestock and poultry manure, and then sprinkle Pseudomonas RST-1 in the aerobic bacterium Pseudomonas. The effective viable count is ≥2×10 9 cfu / g, and the addition amount of the aerobic bacterium is 3.5% of the mass of the modified lincomycin mycelial residue. After stirring well, perform aerobic composting treatment. The aeration rate is 0.7 L / min / kg, turn the pile once every 12 h, and perform composting treatment for 10 days to obtain organic fertilizer.

[0034] Comparative Example 1 A method for producing organic fertilizer using lincomycin mycelial residue, which is different from Example 1 in that irradiated lincomycin mycelial residue powder is used to replace the mixture of alkali lignin-modified lincomycin mycelial residue and livestock and poultry manure, and the other steps are the same as those in Example 1. Specifically: A method for producing organic fertilizer using lincomycin mycelial residue, comprising the following steps: Step 1, placing fresh lincomycin residue (lincomycin content is 0.43wt%) in an oven at a temperature of 120°C, drying until the moisture content is less than 10%, then grinding in a mortar at a speed of 250r / min, and then passing through a 100-mesh sieve to collect powder to obtain lincomycin residue powder; Step 2, spreading the lincomycin residue powder, and treating it using electron beam irradiation technology, with an accelerator energy of 1.8 MeV and a beam current of 50 mA; the irradiation absorbed dose is 80 kGy, to obtain irradiated lincomycin residue powder; Step 3: Mix the irradiated lincomycin residue powder and livestock manure in a mass ratio of 1:2, and then sprinkle Pseudomonas RST-1 from the genus Pseudomonas, with an effective viable count of ≥ 2×10 9 cfu / g, the amount of aerobic bacteria added is 2.5% of the mass of the irradiated lincomycin residue powder. After sufficient stirring, aerobic composting is carried out with an aeration rate of 0.5L / min / kg. The compost is turned every 12 hours. The composting is carried out for 7 days to obtain organic fertilizer.

[0035] Comparative Example 2 A method for producing organic fertilizer using lincomycin residue, which differs from Example 1 in that a mixture of irradiated lincomycin residue powder and alkali lignin is used to replace alkali lignin-modified lincomycin residue, and then mixed with livestock manure, and the remaining steps are the same as those in Example 1. Specifically: Step 1, placing fresh lincomycin residue (lincomycin content is 0.43wt%) in an oven at a temperature of 120°C, drying until the moisture content is less than 10%, then grinding in a mortar at a speed of 250r / min, and then passing through a 100-mesh sieve to collect powder to obtain lincomycin residue powder; Step 2, spreading the lincomycin residue powder, and treating it using electron beam irradiation technology, with an accelerator energy of 1.8 MeV and a beam current of 50 mA; the irradiation absorbed dose is 80 kGy, to obtain irradiated lincomycin residue powder; Step 3: Mix the irradiated lincomycin residue powder, alkali lignin and livestock manure in a mass ratio of 0.7:0.3:2, and then sprinkle Pseudomonas RST-1 from the genus Pseudomonas, with an effective viable count of ≥2×10 9 cfu / g, the amount of aerobic bacteria added is 2.5% of the mass of the modified lincomycin residue. After sufficient stirring, aerobic composting is carried out with an aeration rate of 0.5L / min / kg. The compost is turned every 12 hours. The composting is carried out for 7 days to obtain organic fertilizer.

[0036] Experimental testing For the specific application detection of the organic fertilizers in Example 1 and Comparative Examples 1-2 in pepper cultivation, the location was a certain place in Yancheng, Jiangsu. The variety was Sujiao No. 5, and the sowing time was March 10. The land was deeply plowed and sun-dried 3 times before sowing. The young pepper plants (3-4 true leaves) were planted in the land. The seedbed was 1.1 m wide and the bed soil was 10 cm thick. Fertilization started 7 days after normal watering, and the fertilization amount was 510 g / m 2 , and the harvest was on July 22. The corresponding indicators were measured, and the results are shown in Table 1 below.

[0037] Among them, the lincomycin bacterial residue was from Jiangsu Huiju Pharmaceutical Co., Ltd., provided through cold chain transportation, and stored at 4 °C before use. The lincomycin content (%) = (the mass of lincomycin in the lincomycin bacterial residue organic fertilizer / the mass of lincomycin in the lincomycin bacterial residue before treatment) * 100%.

[0038] Table 1 Cultivation test results of different fertilizers

[0039] It can be seen from the results in Table 1 that the organic fertilizer in Example 1 has a lower lincomycin content, and the growth state of the plants is better. The length and weight of the harvested fruits are both higher than those in Comparative Example 1. This shows that the organic fertilizer prepared in Example 1 of the present invention removes antibiotics more thoroughly, which may be related to the subsequent binding process with alkali lignin in the organic solvent; and it has a better promoting effect on the growth and fruiting of pepper plants.

[0040] In the description of this specification, the description of reference terms such as "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic expressions of the above terms should not be understood as necessarily referring to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples. In addition, those skilled in the art can combine and combine the different embodiments or examples described in this specification.

[0041] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention.

Claims

1. A method for producing organic fertilizer using lincomycin residue, characterized in that: The following steps are involved: Step 1, drying fresh lincomycin residue in an oven, grinding in a mortar, and then sieving to collect powder to obtain lincomycin residue powder; Step 2, spreading the lincomycin residue powder and treating it using electron beam irradiation technology to obtain irradiated lincomycin residue powder; Step 3, mixing the alkali lignin and the sodium hydroxide solution, heating to 50-80°C, stirring for 15-45 minutes, adding the formaldehyde solution, keeping warm and stirring for 1-2 hours, adding sodium sulfite, heating to 80-100°C, keeping warm and stirring for 2-4 hours, cooling to room temperature after the treatment, and discharging in anhydrous ethanol to obtain sulfonated alkali lignin; Step 4, mixing the irradiated lincomycin residue powder and the sulfonated alkali lignin in an organic solvent, adding a catalyst, stirring sufficiently, heating to react, removing the solvent after the reaction is completed, washing with alcohol three times and drying to obtain the alkali lignin modified lincomycin residue; Step 5, mixing the alkali lignin modified lincomycin residue and livestock manure, adding aerobic bacteria, stirring thoroughly, and aerobic composting to obtain organic fertilizer.

2. A method for producing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that, In the step 1, the content of lincomycin in the fresh lincomycin residue is 0.43wt%.

3. A method for producing organic fertilizer by utilizing lincomycin slag according to claim 1, characterized in that, In the step 1, the temperature in the oven is 110-150° C., and the drying is performed until the moisture content is less than 10%; the grinding speed is 200-300 r / min, and the grinding is passed through a 100-mesh sieve.

4. A method for producing organic fertilizer by utilizing lincomycin slag according to claim 1, characterized in that, In step 2, the parameters of the electron beam irradiation technology include: accelerator energy of 1.3-2 MeV, beam current of 30-80 mA; and irradiation absorbed dose of 50-100 kGy.

5. A method for producing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that, In step 3, the mass fraction of the sodium hydroxide solution is 1%-5%, the mass fraction of the formaldehyde solution is 35%-40%; the mass ratio of alkali lignin, formaldehyde, sodium sulfite and sodium hydroxide solution is 1:0.5-0.8:0.2-0.4:5-10.

6. A method for producing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that, In the step 4, the mass ratio of the irradiated lincomycin residue powder, the sulfonated alkali lignin and the organic solvent is 1:0.2-0.7:15-25.

7. A method for producing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that, In step 4, the catalyst is phosphotungstic acid, and the added amount is 2%-7% of the mass of sulfonated alkali lignin; the reaction temperature is 90-130° C., and the mixture is stirred for 2-5 hours.

8. A method for producing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that, In the step 5, the amount of aerobic bacteria added is 1%-3.5% of the mass of the alkali lignin modified lincomycin residue; in the step 5, the mass ratio of the irradiated lincomycin residue powder to the sulfonated alkali lignin is 1:1-3.

9. A method for producing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that, In step 5, during the composting process, the aeration rate is 0.1-1 L / min / kg, the compost is turned every 12 hours, and the composting process lasts 5-10 days.

10. An organic fertilizer produced by using lincomycin residue, characterized in that: The organic fertilizer is prepared by the method according to claim 1.

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