Method for preparing organic fertilizer by utilizing lincomycin mushroom dregs

Abstract

The invention relates to the field of fertilizers, in particular to a method for preparing an organic fertilizer from lincomycin residues, which comprises the following steps: 1, preparing hydroxylated lincomycin residues; step 2, preparing modified lincomycin mushroom dregs; and 3, preparing the organic fertilizer. According to the organic fertilizer prepared by the invention, the utilization of agricultural wastes is more recycled, and the obtained fertilizer is extremely low in lincomycin content and excellent in fertilizer efficiency, can improve the basic soil fertility of soil and improve the quality of agricultural products, and accords with the directions of agricultural sustainable development and green agricultural product production.

Description

Technical Field

[0001] The invention relates to the field of fertilizers, and in particular to a method for preparing organic fertilizer by utilizing lincomycin residue. Background Art

[0002] Antibiotic residues are solid wastes generated during the production of antibiotics. Their main components are mycelium of antibiotic-producing bacteria, unused culture medium, metabolites generated during the fermentation process, degradation products of culture medium, and a small amount of antibiotics. Existing antibiotic residues mainly include tetracyclics, aminoglycosides, peptides, macrolides, antifungals, antituberculosis, etc. Because they contain a lot of nutrients, in the past, antibiotic residues were processed by drying and used as feed or feed additives. Since there are a small amount of antibiotics left in them, long-term consumption by animals will cause drug resistance, zoonoses and other problems. The country has paid more and more attention to the removal of antibiotics in feed, and in 2002, it was included in the "List of Drugs Prohibited from Use in Feed and Animal Drinking Water".

[0003] Lincomycin belongs to the lincosamide antibiotics, which has good thermal stability and can be stable for 1 month at 70℃. At present, the method used by pharmaceutical companies to treat lincomycin residue is to incinerate at a high temperature of 500-600℃. Incineration not only brings huge economic burden to pharmaceutical companies, but also the useful components in the residue are not utilized, resulting in waste of resources. Anaerobic digestion treatment is a complex ecosystem. The raw material ratio has a great influence on the anaerobic digestion effect. It has a high antibiotic removal rate, but a large amount of pollutants are generated, and wastewater and waste residue treatment is difficult. Aerobic composting method can make the residual antibiotics in the residue be utilized, decomposed or converted into other harmless substances by microorganisms under the action of aerobic microorganisms, realize the harmless treatment of the residue, and develop special fertilizers to realize the resource utilization of the residue. Although this method has a good degradation effect, the specific practice has limitations, takes a long time, is difficult to control, and the existing aerobic composting technology does not completely degrade lincomycin in the residue. Summary of the invention

[0004] In view of the problems existing in the prior art, the object of the present invention is to provide a method for preparing organic fertilizer by utilizing lincomycin residue.

[0005] The purpose of the present invention is achieved by the following technical solutions: A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue is first dried, then crushed and sieved, then mixed and soaked with a hydrogen peroxide solution, and ozone is introduced at the same time. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh the phosphorylated glucan and the organic solvent, mix them, stir them thoroughly, add the hydroxylated lincomycin residue, add a small amount of catalyst, heat and stir to react, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain the modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue is mixed with organic fertilizer, water is sprayed to adjust the humidity, and then aerobic bacteria are sprinkled in and composted to obtain usable organic fertilizer.

[0006] Preferably, in the first step, the drying temperature of the lincomycin residue is 100-120° C., and the drying time is 2-8 hours; the crushing and screening is performed through a 50-100 mesh sieve.

[0007] Preferably, in the step 1, the mass fraction of the hydrogen peroxide solution is 5%-15%, and the mass ratio of lincomycin residue to the hydrogen peroxide solution is 1:10-20.

[0008] Preferably, in the first step, the amount of ozone introduced is 200-800 mL / min, and the ozone mixed with the hydrogen peroxide solution is immersed at room temperature for 2-6 hours.

[0009] Preferably, in the second step, the organic solvent is at least one of tetrahydrofuran, dimethyl sulfoxide, dichloromethane and N,N-dimethylformamide.

[0010] Preferably, in the second step, the mass ratio of hydroxylated lincomycin residue, phosphorylated glucan and organic solvent is 1:0.27-0.54:20-30.

[0011] Preferably, in the second step, the catalyst is one of phosphotungstic acid, sulfuric acid and fluoroboric acid, and the added amount is 2%-6% of the mass of the phosphorylated dextran.

[0012] Preferably, in the second step, the reaction temperature is 50-90° C., and the stirring is maintained at this temperature for 2-8 hours.

[0013] Preferably, in the third step, the mass ratio of the modified lincomycin residue to the organic fertilizer is 1-3:1.

[0014] Preferably, in the third step, the organic fertilizer is a mixture of livestock manure and plant straw in a mass ratio of 1-3:1-2.

[0015] Preferably, in step 3, water is sprayed to adjust the humidity to 55%-75%.

[0016] Preferably, in the step 3, the aerobic bacteria are a mixture of one or more of Ochrobactrum sp., Sphingobacterium sp., Pseudomonas sp. and Stenotrophomonas sp.

[0017] Preferably, in step 3, the aerobic bacteria is Pseudomonas RST-1 in the genus Pseudomonas, and the effective viable count is ≥ 2×10 9 cfu / g.

[0018] Preferably, in the third step, the amount of aerobic bacteria added is 1%-3.5% of the mass of the modified lincomycin residue.

[0019] Preferably, in the step 3, during the composting process, the aeration rate is 0.1-1 L / min / kg, the compost is turned every 12 hours, and the composting process lasts for 5-10 days.

[0020] The beneficial effects of the present invention are: 1. The present invention provides a method for preparing organic fertilizer using lincomycin residue. In the method, the lincomycin residue is first treated by combining ozone with hydrogen peroxide solution to kill the antibiotics while obtaining hydroxylated lincomycin residue; then phosphorylated glucan is used to combine with it to obtain modified lincomycin residue; finally, the modified lincomycin residue and organic fertilizer are subjected to aerobic fermentation treatment to finally obtain organic fertilizer. The organic fertilizer prepared by the present invention not only makes the utilization of agricultural waste more resource-oriented, but also has a very low lincomycin content in the obtained fertilizer, excellent fertilizer efficiency, can improve soil basic fertility and improve the quality of agricultural products, and is in line with the direction of sustainable agricultural development and green agricultural product production.

[0021] 2. The fungus residue contains a large amount of mycelium and a variety of active substances such as sugars, organic acids, enzymes, etc. produced during the growth of mycelium. In the present invention, the purpose of using ozone and hydrogen peroxide solution to treat lincomycin fungus residue is to, on the one hand, kill antibiotics such as lincomycin in the fungus residue; on the other hand, use the strong oxidizing property of hydrogen peroxide solution to activate the fungus residue, increase the hydroxyl content on its surface, and then use phosphorylated glucan containing phosphate groups to esterify and combine with it to form fungus residue containing phosphated glucan sodium.

[0022] 3. The phosphorylated glucan used in the present invention is obtained by phosphorylation of glucan and phosphorous acid in molten urea. The phosphorylated glucan is a monobasic phosphate glucan with a phosphate group. The present invention synthesizes the phosphorylated structure by esterification reaction with hydroxylated lincomycin residue, which gives the residue more adsorption activity, so that it can have a certain slow release when combined with other potassium fertilizers and nitrogen fertilizers, thereby further enhancing its fertility, and can significantly improve crop yield and quality, such as pepper, tomato and other crops. DETAILED DESCRIPTION

[0023] The technical solution of the present invention is described below through specific examples. It should be understood that the one or more method steps mentioned in the present invention do not exclude the existence of other method steps before and after the combination step or the insertion of other method steps between these explicitly mentioned steps; it should also be understood that these embodiments are only used to illustrate the present invention and are not used to limit the scope of the present invention. Moreover, unless otherwise specified, the numbering of each method step is only a convenient tool for identifying each method step, and is not intended to limit the order of arrangement of each method step or to limit the scope of the present invention. The change or adjustment of the relative relationship thereof shall also be regarded as the scope of the present invention without substantially changing the technical content.

[0024] In order to better understand the above technical scheme, the exemplary embodiments of the present invention are described in more detail below. Although exemplary embodiments of the present invention are shown, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided in order to enable a more thorough understanding of the present invention and to enable the scope of the present invention to be fully communicated to those skilled in the art.

[0025] The present invention will be further described below in conjunction with the following examples.

[0026] Example 1 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue provided by cold chain transportation is taken, and the lincomycin content accounts for 0.27wt%. The lincomycin residue is dried at 110°C for 5h, crushed and sieved through an 80-mesh sieve, and then mixed with a 10wt% hydrogen peroxide solution, and ozone is introduced at the same time, and the ozone introduction amount is 500mL / min. It is soaked at room temperature for 4h, and the mass ratio of lincomycin residue to hydrogen peroxide solution is 1:15. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh phosphorylated glucan and tetrahydrofuran, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated glucan and tetrahydrofuran is 1:0.41:25, add a small amount of catalyst phosphotungstic acid, heat to 70°C, stir and react for 5h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 2:1. The humidity was adjusted to 65% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the addition amount is 2% of the mass of the modified lincomycin residue, and the composting is carried out with continuous aeration at a rate of 0.5L / min / kg. The pile is turned over every 12 hours, and after 7 days of composting, usable organic fertilizer is obtained.

[0027] Example 2 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue was dried at 100° C. for 2 h, crushed and passed through a 50-mesh sieve, and then mixed with a 5wt% hydrogen peroxide solution, and ozone was introduced at a rate of 800 mL / min. The residue was soaked at room temperature for 2 h, and the mass ratio of the lincomycin residue to the hydrogen peroxide solution was 1:10. After the treatment, the residue was filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue. Step 2, preparing modified lincomycin residue: Weigh phosphorylated glucan and dimethyl sulfoxide, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated glucan and dimethyl sulfoxide is 1:0.27:20, add a small amount of catalyst sulfuric acid, the amount of which is 2% of the mass of phosphorylated glucan, heat to 50°C, stir and react for 2h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 1:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 1:1. The humidity was adjusted to 55% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×109 cfu / g, the addition amount is 1% of the mass of the modified lincomycin residue, and the composting is carried out with continuous aeration at a rate of 0.1L / min / kg. The pile is turned over every 12 hours, and after 5 days of composting, usable organic fertilizer is obtained.

[0028] Example 3 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue was dried at 100° C. for 8 h, crushed and passed through a 50-mesh sieve, and then mixed with an 11 wt % hydrogen peroxide solution, and ozone was introduced at a rate of 300 mL / min. The residue was soaked at room temperature for 3 h, and the mass ratio of the lincomycin residue to the hydrogen peroxide solution was 1:12. After the treatment, the residue was filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue. Step 2, preparing modified lincomycin residue: Weigh phosphorylated glucan and dichloromethane, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated glucan and dichloromethane is 1:0.32:24, add a small amount of catalyst sulfuric acid, the amount of which is 3% of the mass of phosphorylated glucan, heat to 60°C, stir and react for 3h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 1:2. The humidity was adjusted to 60% by spraying water. Then, the aerobic bacteria Pseudomonas RST-1 was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the added amount is 1.5% of the modified lincomycin residue, and composting is carried out with continuous aeration at a rate of 0.7L / min / kg. The pile is turned over every 12 hours, and after 8 days of composting, usable organic fertilizer is obtained.

[0029] Example 4 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue was dried at 120° C. for 8 h, then crushed and passed through a 100-mesh sieve, and then mixed with a 15 wt % hydrogen peroxide solution, and ozone was introduced at a rate of 200 mL / min. The mixture was soaked at room temperature for 6 h, and the mass ratio of the lincomycin residue to the hydrogen peroxide solution was 1:20. After the treatment, the mixture was filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue. Step 2, preparing modified lincomycin residue: Weigh phosphorylated glucan and N,N-dimethylformamide, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated glucan and N,N-dimethylformamide is 1:0.54:30, add a small amount of catalyst fluoroboric acid, the amount of which is 6% of the mass of phosphorylated glucan, heat to 90°C, stir and react for 8h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 3:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 3:2. The humidity was adjusted to 75% by spraying water. Then, the aerobic bacteria Pseudomonas RST-1 was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the added amount is 3.5% of the modified lincomycin residue, and composting is carried out with continuous aeration at a rate of 1L / min / kg. The pile is turned over every 12 hours, and after 10 days of composting, usable organic fertilizer is obtained.

[0030] Example 5 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue provided by cold chain transportation is taken, and the lincomycin content accounts for 0.27wt%. The lincomycin residue is dried at 110°C for 5h, crushed and sieved through an 80-mesh sieve, and then mixed with a 10wt% hydrogen peroxide solution, and ozone is introduced at the same time, and the ozone introduction amount is 500mL / min. It is soaked at room temperature for 4h, and the mass ratio of lincomycin residue to hydrogen peroxide solution is 1:15. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh phosphorylated dextran and tetrahydrofuran, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated dextran and tetrahydrofuran is 1:0.28:25, add a small amount of catalyst phosphotungstic acid, heat to 70°C, stir and react for 5h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 2:1. The humidity was adjusted to 65% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the addition amount is 1.3% of the mass of the modified lincomycin residue, and composting is carried out with continuous aeration at a rate of 0.5L / min / kg. The pile is turned over every 12 hours, and after composting for 7 days, usable organic fertilizer is obtained.

[0031] Example 6 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue provided by cold chain transportation is taken, and the lincomycin content accounts for 0.27wt%. The lincomycin residue is dried at 110°C for 5h, crushed and sieved through an 80-mesh sieve, and then mixed with a 10wt% hydrogen peroxide solution, and ozone is introduced at the same time, and the ozone introduction amount is 500mL / min. It is soaked at room temperature for 4h, and the mass ratio of lincomycin residue to hydrogen peroxide solution is 1:15. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh phosphorylated dextran and tetrahydrofuran, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated dextran and tetrahydrofuran is 1:0.29:25, add a small amount of catalyst phosphotungstic acid, heat to 70°C, stir and react for 5h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 2:1. The humidity was adjusted to 65% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the addition amount is 1.8% of the mass of the modified lincomycin residue, and the composting is treated with continuous aeration at a rate of 0.5L / min / kg. The pile is turned over every 12 hours. After 7 days of composting, usable organic fertilizer is obtained.

[0032] Example 7 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue provided by cold chain transportation is taken, and the lincomycin content accounts for 0.27wt%. The lincomycin residue is dried at 110°C for 5h, crushed and sieved through an 80-mesh sieve, and then mixed with a 10wt% hydrogen peroxide solution, and ozone is introduced at the same time, and the ozone introduction amount is 500mL / min. It is soaked at room temperature for 4h, and the mass ratio of lincomycin residue to hydrogen peroxide solution is 1:15. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh phosphorylated dextran and tetrahydrofuran, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated dextran and tetrahydrofuran is 1:0.33:25, add a small amount of catalyst phosphotungstic acid, heat to 70°C, stir and react for 5h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 2:1. The humidity was adjusted to 65% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the addition amount is 2.1% of the mass of the modified lincomycin residue, and the composting is carried out with continuous aeration at a rate of 0.5L / min / kg. The pile is turned over every 12 hours, and after 7 days of composting, usable organic fertilizer is obtained.

[0033] Example 8 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue provided by cold chain transportation is taken, and the lincomycin content accounts for 0.27wt%. The lincomycin residue is dried at 110°C for 5h, crushed and sieved through an 80-mesh sieve, and then mixed with a 10wt% hydrogen peroxide solution, and ozone is introduced at the same time, and the ozone introduction amount is 500mL / min. It is soaked at room temperature for 4h, and the mass ratio of lincomycin residue to hydrogen peroxide solution is 1:15. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh phosphorylated dextran and tetrahydrofuran, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated dextran and tetrahydrofuran is 1:0.48:25, add a small amount of catalyst phosphotungstic acid, heat to 70°C, stir and react for 5h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 2:1. The humidity was adjusted to 65% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the added amount is 2.4% of the mass of the modified lincomycin residue, and the composting is carried out with continuous aeration at a rate of 0.5L / min / kg. The pile is turned over every 12 hours, and after 7 days of composting, usable organic fertilizer is obtained.

[0034] Example 9 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue provided by cold chain transportation is taken, and the lincomycin content accounts for 0.27wt%. The lincomycin residue is dried at 110°C for 5h, crushed and sieved through an 80-mesh sieve, and then mixed with a 10wt% hydrogen peroxide solution, and ozone is introduced at the same time, and the ozone introduction amount is 500mL / min. It is soaked at room temperature for 4h, and the mass ratio of lincomycin residue to hydrogen peroxide solution is 1:15. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh phosphorylated glucan and tetrahydrofuran, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated glucan and tetrahydrofuran is 1:0.5:25, add a small amount of catalyst phosphotungstic acid, heat to 70°C, stir and react for 5h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 2:1. The humidity was adjusted to 65% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the addition amount is 3% of the mass of the modified lincomycin residue, and the composting is carried out with continuous aeration at a rate of 0.5L / min / kg. The pile is turned over every 12 hours, and after 7 days of composting, usable organic fertilizer is obtained.

[0035] Example 10 A method for preparing organic fertilizer using lincomycin residue comprises the following steps: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue provided by cold chain transportation is taken, and the lincomycin content accounts for 0.27wt%. The lincomycin residue is dried at 110°C for 5h, crushed and sieved through an 80-mesh sieve, and then mixed with a 10wt% hydrogen peroxide solution, and ozone is introduced at the same time, and the ozone introduction amount is 500mL / min. It is soaked at room temperature for 4h, and the mass ratio of lincomycin residue to hydrogen peroxide solution is 1:15. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh phosphorylated glucan and tetrahydrofuran, mix them, stir them thoroughly, add hydroxylated lincomycin residue, the mass ratio of hydroxylated lincomycin residue, phosphorylated glucan and tetrahydrofuran is 1:0.52:25, add a small amount of catalyst phosphotungstic acid, heat to 70°C, stir and react for 5h, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue was mixed with organic fertilizer in a mass ratio of 2:1. The organic fertilizer was a mixture of livestock manure and plant straw in a mass ratio of 2:1. The humidity was adjusted to 65% by spraying water. Then, Pseudomonas RST-1 of the genus Pseudomonas was sprinkled in. The effective live bacteria count was ≥2×10 9 cfu / g, the addition amount is 3% of the mass of the modified lincomycin residue, and the composting is carried out with continuous aeration at a rate of 0.5L / min / kg. The pile is turned over every 12 hours, and after 7 days of composting, usable organic fertilizer is obtained.

[0036] Comparative Example 1 A method for preparing organic fertilizer using lincomycin residue, which differs from Example 1 in that hydroxylated lincomycin residue is used instead of modified lincomycin residue, and the remaining steps are the same as those in Example 1.

[0037] Comparative Example 2 A method for preparing organic fertilizer using lincomycin residue, which differs from Example 1 in that a mixture of hydroxylated lincomycin residue and phosphorylated glucan is used to replace the modified lincomycin residue, the mass ratio of the hydroxylated lincomycin residue to the phosphorylated glucan is 1:0.41, and the remaining steps are the same as those in Example 1.

[0038] Experimental testing The organic fertilizers of Example 1 and Comparative Examples 1-2 were tested for specific application in pepper cultivation. The location was a certain place in Yancheng, Jiangsu Province. The variety was Sujiao No. 5. The peppers were sown in March. The seeds were deep-turned and sun-dried 2-3 times before sowing. The pepper seedlings (3-4 true leaves) were planted in the soil. The width of the seedbed was 1.1m, and the thickness of the soil was 8cm. Fertilization was started 7 days after normal watering. The fertilizer application rate was 300g / m 2 , and waited for harvest in July to measure the corresponding indicators. The results are shown in Table 1 below.

[0039] Table 1 Test results

[0040] Among them, lincomycin residue comes from Jiangsu Huiju Pharmaceutical Co., Ltd., is provided through cold chain transportation, and is stored at 4°C before use. The lincomycin content (%) = (the mass of lincomycin in lincomycin residue organic fertilizer / the mass of lincomycin in lincomycin residue before treatment) * 100%.

[0041] It can be seen from Table 1 that the organic fertilizer prepared in Example 1 of the present invention not only reduces the lincomycin content to a very low level, but also can significantly improve the yield and quality of crops such as peppers, and has a very good yield-increasing effect.

[0042] Although the embodiments of the present invention have been shown and described above, it is to be understood that the above embodiments are exemplary and are not to be construed as limitations of the present invention. A person skilled in the art may change, modify, replace and vary the above embodiments within the scope of the present invention.

Claims

1. A method for preparing organic fertilizer using lincomycin residue, characterized in that: The following steps are involved: Step 1, preparation of hydroxylated lincomycin residue: The lincomycin residue is first dried, then crushed and sieved, then mixed and soaked with a hydrogen peroxide solution, and ozone is introduced at the same time. After the treatment, it is filtered, washed with water three times, and dried to obtain hydroxylated lincomycin residue; Step 2, preparing modified lincomycin residue: Weigh the phosphorylated glucan and the organic solvent, mix them, stir them thoroughly, add the hydroxylated lincomycin residue, add a small amount of catalyst, heat and stir to react, then cool to room temperature, remove the solvent under reduced pressure, wash the reaction product with water until it is neutral, and dry it to obtain the modified lincomycin residue; Step 3, prepare organic fertilizer: The modified lincomycin residue is mixed with organic fertilizer, water is sprayed to adjust the humidity, and then aerobic bacteria are sprinkled in and composted to obtain usable organic fertilizer.

2. A method for preparing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that: In the first step, the drying temperature of the lincomycin residue is 100-120° C., and the drying time is 2-8 hours; the crushing and screening is to pass through a 50-100 mesh sieve.

3. A method for preparing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that: In the first step, the amount of ozone introduced is 200-800 mL / min, the mass fraction of the hydrogen peroxide solution is 5%-15%, the mass ratio of lincomycin residue to the hydrogen peroxide solution is 1:10-20; and the mixed immersion with the hydrogen peroxide solution is carried out at room temperature for 2-6 hours.

4. A method for preparing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that: In the second step, the organic solvent is at least one of tetrahydrofuran, dimethyl sulfoxide, dichloromethane, and N,N-dimethylformamide.

5. A method for preparing organic fertilizer by utilizing lincomycin residue according to claim 1, characterized in that: In the second step, the mass ratio of the hydroxylated lincomycin residue, the phosphorylated glucan and the organic solvent is 1:0.27-0.54:20-30.

6. A method for preparing organic fertilizer by using lincomycin residue according to claim 1, characterized in that: In the second step, the catalyst is one of phosphotungstic acid, sulfuric acid and fluoroboric acid, and the added amount is 2%-6% of the mass of phosphorylated dextran; the reaction temperature is 50-90° C., and the mixture is stirred for 2-8 hours.

7. A method for preparing organic fertilizer by using lincomycin residue according to claim 1, characterized in that: In the third step, the mass ratio of the modified lincomycin residue to the organic fertilizer is 1-3:1; the organic fertilizer is a mixture of livestock manure and plant straw in a mass ratio of 1-3:1-2; and the humidity is adjusted to 55%-75% by spraying water.

8. A method for preparing organic fertilizer using lincomycin residue according to claim 1, characterized in that: In the third step, the aerobic bacteria are a mixture of one or more of Ochrobactrum sp., Sphingobacterium sp., Pseudomonas sp. and Stenotrophomonas sp.

9. A method for preparing organic fertilizer using lincomycin residue according to claim 8, characterized in that: In the third step, the aerobic bacteria is Pseudomonas RST-1 in the genus Pseudomonas, and the effective viable count is ≥ 2×10 9 cfu / g.

10. A method for preparing organic fertilizer using lincomycin residue according to claim 1, characterized in that: In the step 3, the amount of aerobic bacteria added is 1%-3.5% of the mass of the modified lincomycin residue; in the step 3, during the composting process, the aeration rate is 0.1-1L / min / kg, the compost is turned every 12 hours, and the composting process is 5-10 days.

Images