Method for improving germination capability of teosinte seeds

Abstract

The invention provides a method for improving germination capacity of teosinte seeds, which comprises the following steps: pretreating the teosinte seeds, soaking and rinsing to obtain rinsed seeds; the method comprises the following steps: rinsing seeds, adding a radix fici simplicissimae freeze-dried powder solution into the rinsed seeds, carrying out ultrasonic treatment, soaking the seeds in a seed soaking solution, rinsing the soaked seeds, and culturing the rinsed seeds, the seed soaking solution being a purple perilla freeze-dried powder solution or a ramie freeze-dried powder solution. According to the method, the teosinte seeds are placed in the radix fici simplicissimae freeze-dried powder solution for ultrasonic treatment, and are soaked in the purple perilla freeze-dried powder solution or the ramie freeze-dried powder solution, so that the dormancy of the seeds is effectively broken, and the germination rate, the germination uniformity and the antibacterial ability of the seeds are improved; and a basic support is provided for cultivation and germplasm innovation of teosinte. The method is easy and convenient to operate and short in time consumption, the seedlings grow normally after being treated, and the method is suitable for being applied to production practice.

Description

Technical Field

[0001] The present invention relates to the technical field of agriculture, and particularly to a method for improving the germination ability of Zea mays subsp. mexicana seeds. Background Art

[0002] Zea mays subsp. mexicana is a plant of the genus Zea in the family Poaceae, with characteristics such as high yield, excellent quality, and strong regeneration ability. It is an excellent green forage or silage for herbivorous livestock and fish. At the same time, as the ancestor of modern maize, Zea mays subsp. mexicana has played an important role in maize germplasm innovation and genetic improvement.

[0003] Zea mays subsp. mexicana is a multi-tillering plant. The inconsistent flowering periods of each tiller result in uneven maturity of the harvested seeds. Moreover, Zea mays subsp. mexicana seeds have a long dormancy period, and their hard seed coats hinder the absorption of water and nutrients by the caryopsis, making it difficult for the embryo to break through the seed coat, and mildew often occurs during the germination process. The above reasons lead to low germination rate and germination quality of Zea mays subsp. mexicana seeds, and poor emergence uniformity. Therefore, studying how to effectively break seed dormancy, improve seed germination ability, and reduce mildew in seed treatment methods is one of the key problems that must be solved in the current cultivation and germplasm innovation of Zea mays subsp. mexicana. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for improving the germination ability of Zea mays subsp. mexicana seeds. By ultrasonic treatment of Zea mays subsp. mexicana seeds in the freeze-dried powder solution of Ficus hirta Vahl, and soaking treatment in the freeze-dried powder solution of Perilla frutescens (L.) Britt. or Boehmeria nivea (L.) W. T. Wang, and other treatments, the seed dormancy can be effectively broken, the seed germination rate, germination uniformity, and antibacterial ability can be improved, providing a basic support for the cultivation and germplasm innovation of Zea mays subsp. mexicana.

[0005] The present invention solves its technical problems by adopting the following technical solutions.

[0006] The present invention provides a method for improving the germination ability of Zea mays subsp. mexicana seeds, comprising the following steps: S1. After pre-treating Zea mays subsp. mexicana seeds, they are soaked and rinsed to obtain rinsed seeds. S2. Add the freeze-dried powder solution of Ficus hirta Vahl to the rinsed seeds and perform ultrasonic treatment, then soak them in the soaking solution for soaking treatment. After the soaked seeds are rinsed, they are cultured, wherein the soaking solution is the freeze-dried powder solution of Perilla frutescens (L.) Britt. or Boehmeria nivea (L.) W. T. Wang.

[0007] The beneficial effects of the method for improving the germination ability of Zea mays subsp. mexicana seeds in the embodiments of the present invention are: The present invention effectively breaks seed dormancy, improves seed germination rate, germination uniformity and antibacterial ability by subjecting teosinte seeds to ultrasonic treatment in the freeze-dried powder solution of Ficus hirta, and soaking treatment in the freeze-dried powder solution of Perilla frutescens or Boehmeria nivea, etc., providing a basic support for the cultivation and germplasm innovation of teosinte.

[0008] The method for improving the germination ability of teosinte seeds of the present invention is simple to operate and time-saving. After being treated by this method, the seedlings grow normally and are suitable for application in production practice. Specific embodiments

[0009] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Those not specified in the embodiments are carried out according to conventional conditions or conditions recommended by the manufacturer. Those reagents or instruments not specified by the manufacturer can be obtained as conventional products through commercial purchase.

[0010] The method for improving the germination ability of teosinte seeds in the embodiments of the present invention will be specifically described below.

[0011] The embodiments of the present invention provide a method for improving the germination ability of teosinte seeds, including the following steps: S1. After the teosinte seeds are pretreated, they are soaked and rinsed to obtain the rinsed seeds. Before the pretreatment of the teosinte seeds of the present invention, it further includes: placing the teosinte seeds in distilled water and removing the bad seeds and underdeveloped seeds floating on the water surface.

[0012] Further, in a preferred embodiment of the present invention, the pretreatment step is: soaking the teosinte seeds in distilled water at 25-30 °C for 1.2-1.7 h, and stirring once every 30 min during the soaking. Preferably, the soaking time is 1.5 h. The present invention can improve enzyme activity, break dormancy, promote uniform water absorption of the seed coat and improve the emergence uniformity through pretreatment.

[0013] Further, in a preferred embodiment of the present invention, before the soaking, it further includes: placing the pretreated teosinte seeds in sand grains and gently rubbing them until the seed surface is slightly rough. The present invention uses sand grains to treat the seeds, which can enhance permeability and promote seed germination.

[0014] Further, in a preferred embodiment of the present invention, the steps of soaking and rinsing are as follows: Soak the pre-treated teosinte seeds in a potassium permanganate solution with a mass concentration of 0.1% for 10 to 20 minutes, and then stir and rinse with distilled water 5 to 8 times to obtain the rinsed seeds. Preferably, soak the pre-treated teosinte seeds in the potassium permanganate solution for 15 minutes. The present invention uses potassium permanganate to soak the seeds, which can effectively eliminate the pathogenic bacteria on the seed surface and improve the survival rate of the seeds.

[0015] S2. Add the freeze-dried powder solution of Ficus hirta Vahl to the rinsed seeds and perform ultrasonic treatment, and then soak them in the seed soaking solution for seed soaking treatment. After the rinsed seeds obtained from the seed soaking treatment are cultured, wherein the seed soaking solution is the freeze-dried powder solution of Perilla frutescens (L.) Britt. or Boehmeria nivea (L.) W. T. Wang. The present invention can improve the activity of enzymes in the seeds through ultrasonic treatment, promote the conversion of starch and the like into soluble substances, and accelerate seed germination. The freeze-dried powder of Ficus hirta Vahl has certain abilities to break dormancy and inhibit bacteria. The present invention adds the rinsed seeds to the freeze-dried powder solution of Ficus hirta Vahl for ultrasonic treatment, thereby breaking seed dormancy and inhibiting the growth of bacteria and other microorganisms.

[0016] Perilla frutescens (L.) Britt. is an annual herbaceous plant. Natural antioxidants can be extracted from its leaves and stems. Its main components are phenols, flavonoids, sterols, steroid saponins and triterpenoids, which have various biological activities such as antioxidant and anti-inflammatory effects, and have a positive impact on the growth and development of plants. Boehmeria nivea (L.) W. T. Wang contains bioactive phenolic compounds such as chlorogenic acid and flavonoids. These active ingredients can promote seed germination and seedling growth by affecting the metabolic processes in plants. The active ingredients in the freeze-dried powder solutions of Perilla frutescens (L.) Britt. and Boehmeria nivea (L.) W. T. Wang can stimulate the internal metabolic activities of teosinte seeds, improve the germination rate and germination speed of the seeds, and the freeze-dried powder solutions of Perilla frutescens (L.) Britt. and Boehmeria nivea (L.) W. T. Wang also have antioxidant and anti-inflammatory effects, which can protect the seeds from damage by the external environment and improve the stress resistance of the seeds. The present invention uses the freeze-dried powder solution of Perilla frutescens (L.) Britt. or Boehmeria nivea (L.) W. T. Wang to perform seed soaking treatment on teosinte seeds, which can significantly improve the germination ability of the seeds. The germination rate, germination potential and radicle length of the seeds after the seed soaking treatment are significantly improved. In addition, the freeze-dried powder is convenient for storage, and the freeze-dried powder solution is more convenient in actual use than the extract.

[0017] Further, in a preferred embodiment of the present invention, the preparation steps of the freeze-dried powder solution of Ficus hirta Vahl, the freeze-dried powder solution of Perilla frutescens (L.) Britt. and the freeze-dried powder solution of Boehmeria nivea (L.) W. T. Wang are as follows: Lay the fruits of Ficus hirta Vahl, the stems and leaves of Perilla frutescens (L.) Britt. and the stems and leaves of Boehmeria nivea (L.) W. T. Wang on the trays of the freeze-dryer respectively, pre-freeze at -45 °C for 4 h, lower the temperature of the partition plate to -40 °C, lower the temperature of the cold trap to below -70 °C, lower the vacuum degree to below 20 Pa, and run the freeze-drying program. After that, perform pulverization and sieving to obtain the freeze-dried powder of Ficus hirta Vahl, the freeze-dried powder of Perilla frutescens (L.) Britt. and the freeze-dried powder of Boehmeria nivea (L.) W. T. Wang. Among them, the freeze-drying program is as follows: Table 1 Freeze-drying procedure

[0018] Respectively mix the dried powder of Ficus hirta, the dried powder of Perilla frutescens or the dried powder of Boehmeria nivea with distilled water to obtain the liquid of dried powder of Ficus hirta, the liquid of dried powder of Perilla frutescens and the liquid of dried powder of Boehmeria nivea. Among them, the mass-volume ratio of the dried powder of Ficus hirta, the dried powder of Perilla frutescens and the dried powder of Boehmeria nivea to the distilled water is 10 g: 100 mL.

[0019] Furthermore, in a preferred embodiment of the present invention, the step of adding the liquid of dried powder of Ficus hirta to the seeds after rinsing and performing ultrasonic treatment is as follows: put the rinsed seeds into an ultrasonic instrument, add the liquid of dried powder of Ficus hirta, and perform ultrasonic treatment at 300 W and 37 °C for 10 - 20 min. Preferably, the ultrasonic treatment time is 15 min.

[0020] Furthermore, in a preferred embodiment of the present invention, the soaking treatment is carried out in the dark at room temperature, and the soaking treatment time is 22 - 26 h. Preferably, the soaking treatment time is 24 h.

[0021] Furthermore, in a preferred embodiment of the present invention, the step of culturing the seeds after soaking and rinsing is as follows: rinse the soaked seeds with distilled water 3 - 5 times, then put them into a culture dish with a water-retaining cushion layer and culture them in the dark for 3 d, and then put them back into a culture dish with a moisturizing filter paper and place them in a light incubator for culture.

[0022] Furthermore, in a preferred embodiment of the present invention, the water-retaining cushion layer is used after being soaked in the extraction solution of dried powder of Ficus hirta. Among them, in the extraction solution of dried powder of Ficus hirta, the mass-volume ratio of the dried powder of Ficus hirta to distilled water is 1 g: 100 mL, and the extraction time is 24 - 30 h.

[0023] Furthermore, in a preferred embodiment of the present invention, the conditions for dark culture are: culture in the dark at 24 °C, and spray the extraction solution of dried powder of Ficus hirta once a day, and the spraying amount should be such that the water-retaining cushion layer is soaked and there is no flow of extraction solution in the culture dish; the step of placing it in a light incubator for culture is: culture with a light intensity of 1600 lx at 24 °C for 14 h, and then culture in the dark at 16 °C for 10 h, repeat the above steps, and spray distilled water on the culture dish once a day during the culture period, and the spraying amount should be such that the moisturizing filter paper is soaked and there is no water flow in the culture dish. The extraction solution of dried powder of Ficus hirta has an antibacterial effect. Spraying the extraction solution of dried powder of Ficus hirta during the dark culture process in the present invention can reduce the mildew of seeds in the early stage of germination and improve the seed germination rate.

[0024] The method for improving the germination ability of teosinte seeds of the present invention is simple to operate and time-saving. It can effectively break the seed dormancy state, improve the seed germination rate, and has a high germination uniformity. After being treated by this method, the seedlings grow normally and are suitable for application in production practice.

[0025] The features and performance of the present invention will be further described in detail below in conjunction with the embodiments.

[0026] Example 1 This example provides freeze-dried powder of Ficus hirta Vahl, Perilla frutescens (L.) Britt., and Boehmeria nivea (L.) W. T. Wang, which is prepared according to the following steps: (1) Spread the fruits of Ficus hirta Vahl, the stems and leaves of Perilla frutescens (L.) Britt., and Boehmeria nivea (L.) W. T. Wang on the trays of a freeze dryer (GZKJ-10 vacuum freeze dryer, Shanghai Guancai Machinery Technology Co., Ltd.).

[0027] (2) Pre-freeze at -45°C for 4 h, lower the temperature of the partition board to -40°C, lower the temperature of the cold trap to below -70°C, lower the vacuum degree to below 20 Pa, and run the freeze-drying program as shown in Table 1.

[0028] Table 1 Freeze-drying program

[0029] (3) Take out the sample after reaching the drying end point, pulverize it, and sieve it through a 60-mesh sieve to obtain the freeze-dried powder of Ficus hirta Vahl, the freeze-dried powder of Perilla frutescens (L.) Britt., and the freeze-dried powder of Boehmeria nivea (L.) W. T. Wang.

[0030] Example 2 This example provides a method for improving the germination ability of teosinte seeds, including the following steps: (1) Place the teosinte seeds in distilled water, remove the bad seeds and underdeveloped seeds floating on the water surface, and then carry out pretreatment. Among them, the pretreatment method is: soak in distilled water at 25°C for 1.5 h, and stir the seeds once every 30 min during the soaking process.

[0031] (2) Gently rub the pretreated teosinte seeds in sand until the seed surface is slightly rough. Then soak the seeds in a 0.1% potassium permanganate solution for 15 min, and stir and rinse with distilled water 8 times.

[0032] (3) Put the rinsed seeds into an ultrasonic instrument, add the freeze-dried powder solution of Ficus hirta Vahl (the ratio of freeze-dried powder to distilled water is 10 g:100 mL), and carry out ultrasonic treatment at 300 W and 37°C for 15 min.

[0033] (4) Soak the seeds after ultrasonic treatment in the freeze-dried powder solution of Perilla frutescens (L.) Britt. (the ratio of freeze-dried powder to distilled water is 10 g:100 mL), keep them at room temperature in the dark, and the seed soaking treatment time is 24 h.

[0034] (5) After the seed soaking treatment, rinse the seeds 5 times with distilled water, and place them in a petri dish with a water-retaining cushion layer containing the extract of Ficus hirta Vahl freeze-dried powder (the ratio of freeze-dried powder to distilled water is 1 g: 100 mL, and the extract is obtained after extracting for 24 h). Incubate in the dark at 24 °C for 3 d, and spray the extract of Ficus hirta Vahl freeze-dried powder once a day during the incubation period, so that the water-retaining cushion layer is saturated and there is no flow of the extract in the petri dish.

[0035] (6)After the dark incubation is completed, place the seeds in a petri dish with a moisturizing filter paper and place them in a light incubator for incubation. The incubation conditions are as follows: Incubate under light of 1600 lx at 24 °C for 14 h, and then incubate in the dark at 16 °C for 10 h, and repeat this cycle of incubation. Spray distilled water on the petri dish once a day during the incubation period, so that the filter paper is saturated and there is no water flow in the petri dish.

[0036] Example 3 This example provides a method for improving the germination ability of teosinte seeds, including the following steps: (1)Place the teosinte seeds in distilled water, remove the bad seeds and underdeveloped seeds floating on the water surface, and then perform pretreatment. The pretreatment method is as follows: Soak the seeds in distilled water at 25 °C for 1.5 h, and stir the seeds once every 30 min during the soaking process.

[0037] (2)Place the pretreated teosinte seeds in sand and gently rub them until the seed surface is slightly rough. Soak the seeds in a 0.1% potassium permanganate solution for 15 min, and then stir and rinse them 8 times with distilled water.

[0038] (3)Put the rinsed seeds into an ultrasonic cleaner, add the extract of Ficus hirta Vahl freeze-dried powder (the ratio of freeze-dried powder to distilled water is 10 g: 100 mL), and then perform ultrasonic treatment at 300 W and 37 °C for 15 min.

[0039] (4)Soak the seeds after ultrasonic treatment in the extract of Boehmeria nivea L. Gaud. freeze-dried powder (the ratio of freeze-dried powder to distilled water is 10 g: 100 mL), keep them at room temperature in the dark, and the seed soaking treatment time is 24 h.

[0040] (5)After the seed soaking treatment, rinse the seeds 5 times with distilled water, and place them in a petri dish with a water-retaining cushion layer containing the extract of Ficus hirta Vahl freeze-dried powder (the ratio of freeze-dried powder to distilled water is 1 g: 100 mL, and the extract is obtained after extracting for 24 h). Incubate in the dark at 24 °C for 3 d, and spray the extract of Ficus hirta Vahl freeze-dried powder once a day during the incubation period, so that the water-retaining cushion layer is saturated and there is no flow of the extract in the petri dish.

[0041] After the dark incubation is completed, place the seeds in a petri dish lined with moist filter paper and place it in an incubator with light. The incubation conditions are as follows: incubate with light of 1600 lx at 24 °C for 14 h, and then incubate in the dark at 16 °C for 10 h, and repeat this cycle. During the incubation period, spray distilled water on the petri dish once a day, and it is appropriate that the filter paper is soaked and there is no water flowing in the dish.

[0042] Comparative Example 1 This comparative example provides a method for germinating teosinte seeds, including the following steps: (1) Place the teosinte seeds in distilled water, remove the bad seeds and underdeveloped seeds floating on the water surface, and then perform pretreatment. The pretreatment method is: soak in distilled water at 25 °C for 1.5 h, and stir the seeds once every 30 min during the soaking process.

[0043] (2) After pretreatment, place the seeds in a petri dish lined with moist filter paper and incubate in the dark at 24 °C for 3 days. During the incubation period, spray distilled water once a day, and it is appropriate that the moist filter paper is soaked and there is no water flowing in the dish.

[0044] (3) After the dark incubation is completed, place the seeds in a petri dish lined with moist filter paper and place it in an incubator with light. The incubation conditions are as follows: incubate with light of 1600 lx at 24 °C for 14 h, and then incubate in the dark at 16 °C for 10 h, and repeat this cycle. During the incubation period, spray distilled water on the petri dish once a day, and it is appropriate that the filter paper is soaked and there is no water flowing in the dish.

[0045] Comparative Example 2 This comparative example provides a method for germinating teosinte seeds, including the following steps: (1) Place the teosinte seeds in distilled water, remove the bad seeds and underdeveloped seeds floating on the water surface, and then perform pretreatment. The pretreatment method is: soak in distilled water at 25 °C for 1.5 h, and stir the seeds once every 30 min during the soaking process.

[0046] (2) Rub the pretreated teosinte seeds gently in sand until the seed surface is slightly rough. Soak the seeds in a 0.1% potassium permanganate solution for 15 min, and stir and rinse 8 times with distilled water.

[0047] (3) Place the rinsed seeds in an ultrasonic cleaner, add distilled water, and perform ultrasonic treatment at 300 W and 37 °C for 15 min.

[0048] (4) Soak the seeds after ultrasonic treatment in distilled water, place them at room temperature and in the dark, and the soaking treatment time is 24 h.

[0049] After presoaking treatment, rinse with distilled water 5 times, and place in a petri dish with a water-retaining cushion containing distilled water. Incubate in the dark at 24 °C for 3 days, and spray distilled water once a day during the incubation period to ensure that the water-retaining cushion is saturated and there is no flow of leaching solution in the dish.

[0050] (6)After the dark incubation is completed, place the seeds in a petri dish lined with moisturizing filter paper and place it in a light incubator for incubation. The incubation conditions are as follows: Incubate with a light intensity of 1600 lx at 24 °C for 14 h, and then incubate in the dark at 16 °C for 10 h, and repeat this cycle of incubation. Spray distilled water once a day on the petri dish during the incubation period to ensure that the filter paper is saturated and there is no water flow in the dish.

[0051] Test Example 1 In Examples 2 - 3 and Comparative Examples 1 - 2, each example or comparative example was set with 3 replicates, and 30 seeds were placed in each replicate. They were carried out simultaneously in a random arrangement, and the germination potential, germination rate, and mildew rate were respectively counted. Among them, the calculation formulas for the germination potential, germination rate, and mildew rate are as follows: Germination potential (%) = Number of germinated seeds on the 7th day / Number of tested seeds × 100% (1) Germination rate (%) = Number of germinated seeds / Number of tested seeds × 100% (2) Mildew rate (%) = Number of moldy seeds / Number of tested seeds × 100% (3) Table 2 shows the measurement results of the seed germination rate, germination potential, and mildew rate in Examples 2 - 3 and Comparative Examples 1 - 2.

[0052] Table 2 Seed germination rate, germination potential, and mildew rate in different examples and comparative examples

[0053] Note: Lowercase letters indicate significant differences (p < 0.05) among the same measurement indicators. As shown in Table 2, the germination potential of Example 2 was increased by 14.44% and 12.22% compared with Comparative Example 1 and Comparative Example 2 respectively, the germination rate was increased by 13.33% and 10.00% respectively, the mildew rate was decreased by 37.78% and 14.44% respectively, and the radicle was increased by 1.97 cm and 1.20 cm respectively compared with Comparative Example 1 and Comparative Example 2. The germination potential of Example 3 was increased by 28.89% and 26.67% compared with Comparative Example 1 and Comparative Example 2 respectively, the germination rate was increased by 32.22% and 28.89% respectively, the mildew rate was decreased by 36.67% and 13.33% respectively, and the radicle was increased by 4.17 cm and 3.40 cm respectively compared with Comparative Example 1 and Comparative Example 2.

[0054] It can be seen that adopting the technical solution of the present invention can significantly improve the germination rate and germination potential of teosinte seeds, the radicle grows significantly, and it can significantly improve the antibacterial ability of the seeds, effectively promoting the germination ability of the seeds.

[0055] The embodiments described above are some, but not all, of the embodiments of the present invention. The detailed description of the embodiments of the present invention is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention.

Claims

1. A method for improving the germination ability of zea mays seeds, characterized in that: The following steps are involved: S1. After pretreatment, the teosinte seeds are soaked and rinsed to obtain rinsed seeds; S2. Add freeze-dried powder of Eleutherodactyl quinquefolium to the rinsed seeds and perform ultrasonic treatment, and then soak them in a seed soaking liquid for soaking treatment, and culture the soaked seeds after rinsing, wherein the seed soaking liquid is freeze-dried powder of Perilla frutescens or freeze-dried powder of Ramie.

2. The method according to claim 1, characterized in that In step S1, the pretreatment step is: soaking the zea macrophylla seeds in distilled water at 25-30° C. for 1.2-1.7 hours, and stirring once every 30 minutes during the soaking.

3. The method according to claim 1, characterized in that: In step S1, before soaking, the process further includes: placing the pretreated zea macrophylla seeds in sand and gently rubbing the seeds until the surface of the seeds is slightly rough.

4. The method according to claim 1, characterized in that In step S1, the soaking and rinsing steps are: soaking the pretreated zea macrophylla seeds in a potassium permanganate solution with a mass concentration of 0.1% for 10 to 20 minutes, and then stirring and rinsing them with distilled water for 5 to 8 times to obtain the rinsed seeds.

5. The method according to claim 1, characterized in that In step S2, the preparation steps of the lyophilized powder liquid of Prunus armeniaca, the lyophilized powder liquid of Perilla frutescens and the lyophilized powder liquid of Ramie are as follows: The fruits of Prunus armeniaca, the stems and leaves of Perilla frost and the stems and leaves of Ramie were respectively spread flat on the tray of the freeze dryer, pre-frozen at -45°C for 4 h, the partition temperature was reduced to -40°C, the cold trap temperature was reduced to below -70°C, the vacuum degree was reduced to below 20 Pa, and the freeze-dried powder of Prunus armeniaca, Perilla frost and Ramie frost were obtained after crushing and sieving, wherein the freeze-drying procedure is: The lyophilized powder of Eleutherodendron chinense, the lyophilized powder of Perilla frost or the lyophilized powder of Ramie are respectively mixed with distilled water to obtain the lyophilized powder liquid of Eleutherodendron chinense, the lyophilized powder liquid of Perilla frost and the lyophilized powder liquid of Ramie, wherein the mass volume ratio of the lyophilized powder of Eleutherodendron chinense, the lyophilized powder of Perilla frost and the lyophilized powder of Ramie to the distilled water is 10g:100mL.

6. The method according to claim 1, characterized in that In step S2, the step of adding the freeze-dried powder of Eleutherodactyl quinquefolius to the rinsed seeds and performing ultrasonic treatment is: putting the rinsed seeds into an ultrasonic instrument, adding the freeze-dried powder of Eleutherodactyl quinquefolius, and then performing ultrasonic treatment at 300W and 37°C for 10 to 20 minutes.

7. The method according to claim 1, characterized in that In step S2, the seed soaking treatment is carried out at room temperature in the dark, and the seed soaking treatment time is 22 to 26 hours.

8. The method according to claim 5, characterized in that In step S2, the step of culturing the soaked seeds after rinsing is as follows: rinsing the soaked seeds with distilled water for 3 to 5 times, placing them in a culture dish covered with a water-retaining cushion layer and culturing them in the dark for 3 days, then placing them back in a culture dish covered with a moisturizing filter paper, and placing them in a light incubator for culturing.

9. The method according to claim 8, characterized in that The water-retaining cushion layer is used after being soaked in an extract of freeze-dried powder of Eleutherodactylus indica. In the extract of freeze-dried powder of Eleutherodactylus indica, the mass volume ratio of freeze-dried powder of Eleutherodactylus indica to distilled water is 1 g:100 mL, and the extraction time is 24 to 30 h.

10. The method according to claim 9, characterized in that The dark culture conditions are: dark culture at 24°C, and spraying the five-fingered peach freeze-dried powder extract once a day, and the spraying amount is suitable for the water-retaining cushion layer to be soaked and no extract flows in the culture dish; the step of placing in a light incubator for culture is: culturing at 24°C with 1600lx light for 14 hours, and then culturing in the dark at 16°C for 10 hours, repeating the above steps, and during the culture period, spraying distilled water on the culture dish once a day, and the spraying amount is suitable for the moisture-retaining filter paper to be soaked and no water flows in the culture dish.

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