Method for improving cold resistance and yield of cistanche tubulosa under Chinese tamarisk forest in Yellow River delta region

Abstract

The invention belongs to the field of under-forest planting, and particularly relates to a method for improving cold resistance and yield of cistanche tubulosa under a Chinese tamarisk forest in the Yellow River Delta district. According to the method, the activity and resistance of host root systems are improved through root system root cutting treatment and treatment liquid spraying; the inoculation resistance and the survival rate of the cistanche tubulosa are promoted through gibberellin seed dressing, three-layer sowing and Hoagland nutrient solution spraying; by means of measures such as near-natural matrix seed dressing, additional application of microbial fertilizer and farmyard manure to soil and later covering treatment, soil nutrition is enriched, the heat preservation performance of the soil is improved, the cold resistance and yield of the cistanche tubulosa are improved, and the cold resistance of the cistanche tubulosa of which the soil is broken in autumn and winter in the current year is improved by spraying exogenous calcium. By means of the measures, the soil temperature is increased by 2-4 DEG C, the cold resistance of the cistanche tubulosa under the Chinese tamarisk forest is improved by 63% or above, the yield is increased by 40% or above, and the method has important significance on industrialized and large-scale cultivation of the cistanche tubulosa under the Chinese tamarisk forest in the Yellow River delta area.

Description

Technical Field

[0001] The present invention belongs to the field of under-forest planting, and particularly relates to a method for improving the cold resistance and yield of Cistanche tubulosa under Tamarix chinensis in the Yellow River Delta region. Background Art

[0002] The statements herein only provide background art related to the present invention and do not necessarily constitute prior art.

[0003] Cistanche tubulosa is a perennial parasitic herb of the genus Cistanche in the family Orobanchaceae, mainly distributed in the Taklimakan Desert and its surrounding areas in Xinjiang Uygur Autonomous Region, and often parasitizes on the roots of plants of the genus Tamarix. The climate in this area is arid, with little rainfall, large evaporation, and long sunshine hours. This special growth environment makes Cistanche tubulosa have strong drought tolerance but poor cold tolerance.

[0004] Tamarix chinensis Lour. is a deciduous large shrub or small tree of the genus Tamarix in the family Tamaricaceae. As the main woody plant constructive species in saline-alkali areas, it has physiological characteristics such as salt tolerance, drought tolerance, and barren tolerance, and has now become the main ecological restoration tree species in the saline-alkali area of the Yellow River Delta. At present, relevant studies on inoculating Cistanche tubulosa with Tamarix chinensis have been successively carried out in the Yellow River Delta region. Among them, Yang Qingshan et al. discussed environmental factors such as soil moisture, temperature and measure factors (cultivation depth, host root distribution) that affect the yield of Cistanche tubulosa, and clarified the influencing factors; Hou Lei et al. investigated the inoculation rate and growth status of Cistanche tubulosa in the Yellow River Delta, and concluded that it is feasible to use wild Tamarix chinensis to inoculate and produce Cistanche tubulosa in the saline-alkali land of the Yellow River Delta.

[0005] As an intersection zone between the two major ecosystems of land and sea, the Yellow River Delta region has a relatively unique ecological type. The soil in the region is severely salinized, the groundwater level is low, the winter climate is dry and cold, and cold winds are frequent. Some studies have shown that the water content of Cistanche tubulosa is as high as over 80%, and the critical cold tolerance temperature is between -2.5°C and -3°C. While the lowest winter temperature in the Yellow River Delta region is mostly around -5°C to -10°C, the soil temperature is mostly between -2°C and -6°C, and there are prominent characteristics such as high soil humidity, long low-temperature duration, and deep frozen soil layer. From this, it can be concluded that freezing damage is the main influencing factor affecting the cultivation and yield of Cistanche tubulosa in the Yellow River Delta region. In addition, the harvesting time of Cistanche tubulosa in this area is around late April to mid-May. Therefore, improving the cold resistance of Cistanche tubulosa itself and enhancing the soil heat preservation are of great significance for the cultivation of Cistanche tubulosa.

[0006] At present, although there are certain research reports on cultivating Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta, the research mainly focuses on conventional cultivation methods, and there is no report on improving the cold resistance of Cistanche tubulosa under Tamarix chinensis forests in this area. Summary of the Invention

[0007] To overcome the deficiencies of the above-mentioned prior art, the present invention provides a method for improving the cold resistance and yield of Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta region. Aiming at the prominent problems such as poor cold resistance and low yield of Cistanche tubulosa, through various measures such as root treatment, seed treatment, sowing treatment, soil treatment, covering treatment and tending management, the root activity of the host Tamarix chinensis is improved, and the inoculation rate, yield and cold resistance of Cistanche tubulosa are promoted, which is of great significance to the industrialization and large-scale development of Cistanche tubulosa in the Yellow River Delta region.

[0008] To achieve the above object, the present invention adopts the following technical solutions:

[0009] A method for improving the cold resistance and yield of Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta region, including root treatment, seed treatment, sowing depth selection, and soil treatment, wherein,

[0010] The method of root treatment is: one year before inoculating Cistanche tubulosa, with the tree trunk as the benchmark, at 35-40 cm on both sides of the row between the trees, use a sharp tool to cut off the lateral roots and fibrous roots vertically downward by 40-50 cm, and at the same time spray the treatment liquid at the cut roots;

[0011] The method of seed treatment is: on the day of sowing, soak the seeds of Cistanche tubulosa in warm water at 30-35 °C for 5-6 hours, then quickly rinse with 75% alcohol for 1-2 minutes, then rinse with running water for 2-3 times, and finally mix the seeds with gibberellin at a concentration of 18-20 mg.kg -1 ;

[0012] The method of sowing treatment is: determine the sowing depth. From late April to early June, dig a trench at 35-40 cm away from the main trunk of Tamarix chinensis, with a width of 25-30 cm. According to the root distribution characteristics of Tamarix chinensis during the root treatment process, determine the trench depth to be 50-60 cm; sow by a three-layer sowing method;

[0013] The method of soil treatment is: after sowing in the current year, in July-August, combine with cultivating and loosening the soil, apply 2-3 kg of microbial bacterial fertilizer per mu, and the cultivating depth should not be too shallow, controlled at 15-20 cm; from mid-October to early November, apply 700-800 kg of farmyard manure per mu.

[0014] In some embodiments, it further includes the step of selecting a tamarisk plantation. The method for selecting a tamarisk plantation is as follows: select a Chinese tamarisk forest land with a soil texture of light loam or sandy soil, the perennial groundwater level should be below 1.2 m, the Chinese tamarisk is required to grow well, have well-developed roots, and be free of pests and diseases, and the forest age is 3 - 5 years.

[0015] In some embodiments, in the method of root treatment, the treatment solution is prepared from glucose, acetic acid, and glycine. Among them, based on the weight of the added water, the mass percentages of each component are: glucose 45%, acetic acid 15%, and glycine 40%.

[0016] In some embodiments, in the method of seed treatment, a substrate is also added when gibberellin is used for seed dressing. Among them, due to the small size of the cistanche tubulosa seeds, the mass ratio of the seeds to the substrate is 1:4 - 1:6; the substrate is prepared from peat soil, surface layer humus soil of the tamarisk forest, perlite, and bagasse. Among them, the mass ratio of peat soil, surface layer humus soil of the tamarisk forest, perlite, and bagasse is 4:3:2:1.

[0017] In some embodiments, in the method of sowing treatment, the three-layer sowing method is specifically as follows: spread the seeds mixed with the substrate on the bottom layer of the ditch, sow another layer after filling 10 cm of soil, and sow a total of three layers upward. For each layer sown, it is sprayed with a 1 / 2 concentration of modified Hoagland nutrient solution. After the third layer of sowing is completed, a layer of 2 - 3 cm of substrate is spread on the upper layer. The substrate is prepared from peat soil, surface layer humus soil of the tamarisk forest, perlite, and bagasse. Among them, the mass ratio of peat soil, surface layer humus soil of the tamarisk forest, perlite, and bagasse is 4:3:2:1.

[0018] Preferably, the sowing amount of cistanche tubulosa is controlled at 260 - 300 g / 666.7 m 2 .

[0019] In some embodiments, it further includes the step of covering treatment. The method of covering treatment is as follows: in late November of the sowing year, with the main trunk of the Chinese tamarisk as the center, extend outward to near the drip line of the tree crown, and use a black weed-proof cloth for surface covering, and remove the surface covering in late March of the following year in a timely manner.

[0020] In some embodiments, it further includes the steps of irrigation, soil loosening, weeding, pruning, and pest control. Among them, when pruning, on the basis of following the conventional pruning principles of branches, the branches between rows are severely retracted and pruned, and the length is cut by 1 / 2 - 3 / 4.

[0021] In some embodiments, for the plots near the coast or the windward side, windbreak barriers should be established around or in the Chinese tamarisk forest land in winter. The windbreak barriers are made of straw or straw mats, with a height set at 100 - 120 cm and buried 30 cm underground.

[0022] In some embodiments, for the Cistanche tubulosa that emerges from the soil in the autumn and winter of the current year, exogenous calcium is also sprayed on its surface.

[0023] In some embodiments, for the Cistanche tubulosa that emerges from the soil in the autumn and winter of the current year, a 15.0 mmol·L -1 concentration of CaCl 2 solution is used to spray the Cistanche tubulosa emerging from the soil twice, with a 10 - 15-day interval between the two sprays.

[0024] The above one or more technical solutions have the following beneficial effects:

[0025] In view of the prominent problems such as poor cold resistance and low yield of Cistanche tubulosa under Tamarix chinensis in the Yellow River Delta region, the present invention proposes a method for improving the yield and cold resistance of Cistanche tubulosa under Tamarix chinensis in the Yellow River Delta region. By means of root cutting treatment and spraying treatment liquid, the root activity and resistance of the host are improved; by soaking seeds with gibberellin, sowing in three layers and spraying Hoagland nutrient solution, the inoculation resistance and survival rate of Cistanche tubulosa are promoted; by measures such as mixing seeds with near-natural substrate, increasing microbial bacterial fertilizer, farmyard manure in the soil and post-covering treatment, the soil nutrition is enriched, the soil heat preservation performance is improved, and the cold resistance and yield of Cistanche tubulosa are increased. In addition, for the Cistanche tubulosa that emerges from the soil in the autumn and winter of the current year, exogenous calcium is sprayed to increase its cold resistance. Through the above measures, the soil temperature is increased by 2 - 4°C, the cold resistance ability of Cistanche tubulosa under Tamarix chinensis is increased by more than 63%, and the yield is increased by more than 40%, which is of great significance for the industrialization and large-scale cultivation of Cistanche tubulosa under Tamarix chinensis in the Yellow River Delta region. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] The accompanying drawings forming a part of this invention are used to provide a further understanding of the invention. The schematic embodiments and descriptions thereof of the invention are used to explain the invention and do not constitute an improper limitation of the invention.

[0027] Figure 1 It shows root cutting and spraying treatment liquid in Example 1 of the present invention;

[0028] Figure 2 It shows the situation of single Tamarix chinensis plant inoculated with Cistanche tubulosa in Example 1 of the present invention;

[0029] Figure 3 It shows the situation of Cistanche tubulosa emerging from the soil under Tamarix chinensis in April after overwintering in Example 1 of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs.

[0031] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular forms are also intended to include the plural forms. In addition, it should also be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0032] Example 1:

[0033] Cistanche tubulosa was inoculated under Tamarix chinensis in China. The experiment was divided into two groups, with each group having an inoculation length of 10 m and a seed dosage of 16.2 g. The first group was the experimental group, and the second group was the control group. The seeds of Cistanche tubulosa were sourced from Hotan Prefecture, Xinjiang Uygur Autonomous Region.

[0034] The experimental group carried out cold resistance treatment using the following method:

[0035] A method for improving the cold resistance and yield of Cistanche tubulosa under Tamarix chinensis in the Yellow River Delta region, comprising the following steps:

[0036] (1) Selection of Tamarix chinensis plantation: Select a Tamarix chinensis forest land with a soil texture of light loam or sandy soil, and the perennial groundwater level should be below 1.2 m. Tamarix chinensis is required to grow well, have well-developed roots, and be free of pests and diseases. The forest age is preferably 3 - 5 years. The experimental forest in this example is a 4-year-old 'Luchen No. 1' Tamarix chinensis forest with a plant spacing of 3 m × 2 m.

[0037] (2) Root treatment: One year before inoculating Cistanche tubulosa, with the tree trunk as the benchmark, use a sharp tool to cut off the lateral roots and fibrous roots vertically downward 40 - 50 cm at 35 - 40 cm on both sides of the row. The incision should be smooth to facilitate wound healing and the growth of fine roots. At the same time, spray the treatment liquid ( Figure 1 ) on the cut roots. The treatment liquid is prepared from glucose, acetic acid, and glycine. Among them, based on the weight of the added water, the mass percentages of each component are: glucose 45%, acetic acid 15%, and glycine 40%. By increasing the relative contents of some substances such as sugars, organic acids, and amino acids, the concentration and secretion ability of root exudates are improved, thereby promoting the growth of fine roots and enhancing their stress resistance.

[0038] (3) Seed treatment: On the day of sowing, soak the seeds of Cistanche tubulosa in warm water at 30 - 35 °C for 5 - 6 hours, then quickly rinse them with 75% alcohol for 1 - 2 minutes, then rinse them with running water 2 - 3 times, and finally use a concentration of 18 - 20 mg·kg -1Seed dressing with gibberellin is carried out to promote the germination of Cistanche tubulosa seeds and the formation of haustoria, increasing the inoculation success rate and stress resistance. Since the 1000-seed weight of Cistanche tubulosa seeds is relatively small, a substrate can be added during gibberellin seed dressing. Among them, the mass ratio of seeds to the substrate is 1:4 to facilitate subsequent sowing. The substrate is prepared from peat soil, surface layer humus soil of Tamarix ramosissima forest, perlite and bagasse. Among them, the mass ratio of peat soil, surface layer humus soil of Tamarix ramosissima forest, perlite and bagasse is 4:3:2:1.

[0039] (4) Sowing treatment: From late April to early June, a trench is dug 35 - 40 cm away from the main trunk of Tamarix chinensis, with a width of 25 - 30 cm. According to the root distribution characteristics of Tamarix chinensis during the root treatment process, the depth of the trench is determined to be 50 - 60 cm. Immediately afterwards, the seeds mixed with the substrate are sown in a layer at the bottom of the trench. After filling 10 cm of soil, another layer is sown, and a total of three layers are sown upwards. For each layer sown, a 1 / 2 concentration of modified Hoagland nutrient solution is used for spraying treatment to improve the stress resistance of the seeds by supplementing nutrients from the outside, promoting the survival rate and yield of subsequent inoculation. After the third layer of sowing is completed, a layer of 2 - 3 cm of substrate is spread on the upper layer. The substrate is prepared from peat soil, surface layer humus soil of Tamarix ramosissima forest, perlite and bagasse. Among them, the mass ratio of peat soil, surface layer humus soil of Tamarix ramosissima forest, perlite and bagasse is 4:3:2:1. The near-natural substrate is used to trigger seed germination, improve the cold resistance of seedlings after inoculation and soil heat preservation. The sowing rate of Cistanche tubulosa is controlled at 260 - 300 g / 666.7 m 2 , and finally the trench is backfilled and compacted.

[0040] (5) Soil treatment: After sowing in the current year, from July to August, 2 - 3 kg of microbial bacterial fertilizer is applied per mu in combination with mid - cultivation and soil loosening. The depth of mid - cultivation should not be too shallow, controlled at 15 - 20 cm. The application of bacterial fertilizer promotes the absorption of soil nutrient elements by the roots of Tamarix chinensis and the secretion of key parasitic substances, improving root vitality and stress resistance, thereby enhancing the growth and cold resistance of Cistanche tubulosa. The organic matter in this bacterial fertilizer is ≥50%, humus is ≥20%, N + P 2 O 5 +K 2 O≥4%, total viable bacteria count ≥200 million / g, effective viable bacteria count ≥50 million / g. Thereafter, from mid - October to early November, 700 - 800 kg of farmyard manure is additionally applied per mu, which can increase the heat absorption capacity and water retention capacity of the soil. Under the action of the microbial bacterial fertilizer, the organic matter in the winter farmyard manure decomposes rapidly and continuously releases heat energy, thereby increasing the soil temperature and providing nutrients for the subsequent growth of Cistanche tubulosa.

[0041] (6) Coverage treatment: In late November of the sowing year, with the main trunk of Tamarix chinensis as the center, expand outward to near the drip line of the tree crown, and surface coverage can be carried out to increase soil temperature. The coverage should be carried out with black weed-proof cloth, which can form a heat preservation layer by absorbing solar heat radiation and soil heat radiation, so as to weaken soil evaporation and heat dissipation. In addition, the black weed-proof cloth should be removed in late March of the following year to avoid affecting the emergence and growth of Cistanche tubulosa.

[0042] (7) Cultivation and management measures: According to the growth characteristics of Tamarix chinensis and Cistanche tubulosa under the forest, irrigation, soil loosening, weeding, pruning and pest control work should be carried out regularly.

[0043] Among them, to reduce the risk of freezing damage, irrigation is prohibited after the end of November to prevent reducing the aeration of the soil and affecting the respiration of the roots of Tamarix chinensis and Cistanche tubulosa. In addition, when pruning, on the basis of following the conventional pruning principles of branches, the branches between rows can be severely retracted and pruned, and shortened by 1 / 2 - 3 / 4 to increase the light transmittance between rows, so as to achieve the effects of increasing ground temperature and facilitating later harvesting. In winter, for the plots near the coast or the windward side, windbreaks should be established around or in the Tamarix chinensis forest land to reduce the invasion of cold wind on Cistanche tubulosa under the forest. The windbreaks can be made of straw or straw mats, with a height of 100 - 120 cm and buried 30 cm underground. At the same time, for the Cistanche tubulosa that emerges from the ground in the autumn and winter of the current year, on the basis of doing the above cultivation and management well, spray the emerged Cistanche tubulosa with a 15.0 mmol·L -1 concentration of CaCl 2 solution twice, with an interval of 10 - 15 days between the two times.

[0044] The control group was treated by the conventional method of inoculating Cistanche tubulosa:

[0045] (1) Selection of Tamarix chinensis plantation: Select a Tamarix chinensis forest land with a soil texture of light loam or sandy soil, and the perennial groundwater level should be below 1.2 m. Tamarix chinensis is required to grow well, have well-developed roots and no diseases and pests, and the forest age is preferably 3 - 5 years. The experimental forest in this example is a 4-year-old 'Luchen No. 1' Tamarix chinensis forest, with a plant spacing of 3 m × 2 m.

[0046] (2) Root treatment: No root cutting treatment was carried out.

[0047] (3) Seed treatment: On the sowing day, soak the seeds of Cistanche tubulosa in warm water at 30 - 35 °C for 5 - 6 hours, then quickly rinse them with 75% alcohol for 1 - 2 minutes, and then rinse them with running water for 2 - 3 times. No gibberellin treatment was carried out, and the seeds were directly mixed with the substrate in a ratio of 1:4. Among them, the substrate is river sand.

[0048] (4) Sowing treatment: From late April to early June, dig a trench at a distance of 35 - 40 cm from the trunk of Tamarix chinensis, with a width of 25 - 30 cm. Based on the root distribution characteristics of Tamarix chinensis, determine the trench depth to be 50 cm. Control the sowing rate of Cistanche tubulosa at 260 - 300 g / 666.7 m 2 , and finally backfill and compact the trench.

[0049] (5) Soil treatment: After sowing in the same year, in July - August, apply 2 - 3 kg of ordinary compound fertilizer per mu in combination with cultivating and loosening the soil. The cultivating depth should not be too shallow, controlled at 15 - 20 cm. Thereafter, from mid - October to early November, apply an additional 700 - 800 kg of farmyard manure per mu, which can increase the soil's heat absorption capacity and water retention capacity.

[0050] (6) Cover treatment: In late November of the sowing year, with the trunk of Tamarix chinensis as the center, expand outward to near the drip line of the tree crown, and conduct surface covering to increase soil temperature, mainly using rice straw for covering.

[0051] (7) Cultivation and management measures: According to the growth characteristics of Tamarix chinensis and Cistanche tubulosa under the forest, regularly carry out conventional cultivation such as irrigation, soil loosening, weeding, pruning, and pest control.

[0052] Among them, the inoculation situation of Cistanche tubulosa on individual plants of Tamarix chinensis in the experimental group is as Figure 2 shown, and the emergence situation of Cistanche tubulosa under the Tamarix chinensis forest in April after overwintering is as Figure 3 shown.

[0053] Using the method of the present invention and the conventional method, measure the number of emerged plants (measured over 5 meters), yield, and soil temperature at 40 cm in January after inoculating Cistanche tubulosa under the Tamarix chinensis forest. Calculate the semi - lethal temperature through conductivity.

[0054] According to the method described in this application, 46 plants of Cistanche tubulosa emerged after 1 year of inoculation, with a yield of 1109.3 kg per mu and a soil temperature of 1.2 °C, as shown in Table 1.

[0055] Table 1

[0056]

[0057] Example 2:

[0058] The test forest is a 4 - year - old Tamarix chinensis forest of 'Luchen No.1', with a plant spacing of 3 m × 2 m.

[0059] Experimental methods: The root cutting + spraying treatment solution, gibberellin seed dressing treatment, three-layer sowing + Hoagland nutrient solution treatment in the experimental group method of Example 1 were used separately. Other treatment steps were conventional treatment methods. The untreated (CK) method was the same as the control group (conventional method) in Example 1. Among them, the inoculation length, seed dosage, and seed source of Cistanche tubulosa in the above different treatment groups were the same as those in Example 1.

[0060] One year after inoculation of Cistanche tubulosa under Tamarix chinensis, the number of unearthed plants (measured at 5 m), yield measurement, and soil temperature at 40 cm in January were measured.

[0061] As shown in Table 2, when using only the root cutting + spraying treatment solution alone, 30 plants of Cistanche tubulosa were unearthed, and the yield per mu reached 647.2 kg; when using only the gibberellin seed dressing treatment alone, 32 plants of Cistanche tubulosa were unearthed, and the yield per mu reached 716.5 kg; when using only the three-layer sowing + Hoagland nutrient solution treatment alone, 35 plants of Cistanche tubulosa were unearthed, and the yield per mu reached 802.4 kg.

[0062] Table 2

[0063]

[0064] Example 3:

[0065] The test forest was a 4-year-old 'Luchen No. 1' Tamarix chinensis forest, with a plant spacing of 3 m × 2 m.

[0066] Experimental methods: Different sowing treatment methods and soil treatment methods were selected. After sowing, laying the substrate, applying only microbial fertilizer, applying only farmyard manure, and applying microbial fertilizer + farmyard manure in the experimental group method of Example 1 were used separately. Other treatment steps were conventional treatment methods. Cistanche tubulosa was inoculated. The untreated (CK) method was the same as the control group (conventional method) in Example 1. Among them, the inoculation length, seed dosage, and seed source of Cistanche tubulosa in the above different treatment groups were the same as those in Example 1.

[0067] One year after inoculation of Cistanche tubulosa under Tamarix chinensis, the number of unearthed plants (measured at 5 m), yield measurement, and soil temperature at 40 cm in January were measured.

[0068] As shown in Table 3, according to the single methods described above, when using the substrate laid after sowing, 32 plants of Cistanche tubulosa were unearthed, the yield per mu reached 688.7 kg, and the soil temperature reached 0.2 °C; when using only microbial fertilizer, 29 plants of Cistanche tubulosa were unearthed, the yield per mu reached 620.4 kg, and the soil temperature reached -0.6 °C; when using only farmyard manure, 26 plants of Cistanche tubulosa were unearthed, the yield per mu reached 594.2 kg, and the soil temperature reached -0.3 °C; when using microbial fertilizer + farmyard manure in combination, 31 plants of Cistanche tubulosa were unearthed, the yield per mu reached 769.5 kg, and the soil temperature reached 0.5 °C.

[0069] Table 3

[0070]

[0071] Example 4:

[0072] The experimental forest is a 4-year-old Chinese tamarisk forest of 'Luchen No. 1', with a plant spacing of 3m×2m.

[0073] Experimental method: Select different covering treatment methods, and separately adopt the black weed-proof cloth and soil covering methods in the experimental group method of Example 1. Other treatment steps are conventional treatment methods. Inoculate Cistanche tubulosa, and the untreated (CK) method is the same as the control group (conventional method) in Example 1. Among them, the inoculation length, seed dosage, and seed source of Cistanche tubulosa in the above different treatment groups are the same as those in Example 1.

[0074] Measure the number of Cistanche tubulosa unearthed after one year of inoculation under Chinese tamarisk (measured at 5 meters), the yield measurement situation, and the soil temperature at 40 cm in January.

[0075] As shown in Table 4, according to the single method described above, using black weed-proof cloth covering, 27 Cistanche tubulosa were unearthed, the yield per mu reached 581.2 kg, and the soil temperature reached 1.2°C; using soil covering, 24 Cistanche tubulosa were unearthed, the yield per mu reached 466.4 kg, and the soil temperature reached -0.7°C.

[0076] Table 4

[0077]

[0078] Example 5:

[0079] The experimental forest is a 4-year-old Chinese tamarisk forest of 'Luchen No. 1', with a plant spacing of 3m×2m.

[0080] Experimental method: Select different forest tending management measures, and separately adopt the winter irrigation, pruning, and windbreak setting methods in the experimental group method of Example 1. Other treatment steps are conventional treatment methods. Inoculate Cistanche tubulosa, and the untreated (CK) method is the same as the control group (conventional method) in Example 1. Among them, the inoculation length, seed dosage, and seed source of Cistanche tubulosa in the above different treatment groups are the same as those in Example 1.

[0081] Measure the number of Cistanche tubulosa unearthed after one year of inoculation under Chinese tamarisk (measured at 5 meters), the yield measurement situation, and the soil temperature at 40 cm in January.

[0082] As shown in Table 5, according to a certain method described in this patent application, by using winter irrigation, 8 Cistanche tubulosa plants emerged, the yield per mu reached 194.3 kg, and the soil temperature reached -4.2 °C; by using pruning, 20 Cistanche tubulosa plants emerged, the yield per mu reached 401.6 g, and the soil temperature reached -2.4 °C; by using windbreak setting, 21 Cistanche tubulosa plants emerged, the yield per mu reached 324.4 g, and the soil temperature reached -1.9 °C.

[0083] Table 5

[0084]

[0085] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for improving the cold resistance and yield of Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta, characterized in that: Including root treatment, seed treatment, sowing depth selection, soil treatment, among which, The method of root treatment is: one year before inoculation of Cistanche tubulosa, using the trunk of Tamarix chinensis as the reference, cut off the lateral roots and fibrous roots 35-40 cm on both sides of the row and 40-50 cm vertically from the ground, and spray the treatment liquid on the broken roots at the same time; The seed treatment method is as follows: on the day of sowing, soak the seeds of Cistanche tubulosa at 30-35℃ for 5-6 hours, rinse with 75% alcohol for 1-2 minutes, rinse with running water 2-3 times, and use a concentration of 18-20mg.kg -1 Gibberellic acid is used for seed dressing; The sowing treatment method is: from late April to early June, dig a furrow 35 to 40 cm away from the main trunk of Chinese Tamarix, with a width of 25 to 30 cm and a depth of 50 to 60 cm; sow in a three-layer sowing method; The method of soil treatment is: after sowing in the same year, from July to August, apply 2 to 3 kg of microbial fertilizer per mu in combination with tillage and loosening the soil, and control the tillage depth at 15 to 20 cm; from mid-October to early November, apply 700 to 800 kg of farmyard manure per mu.

2. The method according to claim 1, characterized in that The method also includes the step of selecting a Tamarix artificial forest. The method for selecting a Tamarix artificial forest is as follows: select a Chinese Tamarix forest land with a soil texture of light loam or sandy soil, a groundwater level below 1.2m, and a forest age of 3-5 years.

3. The method according to claim 1, characterized in that In the root treatment method, the treatment solution is prepared from glucose, acetic acid and glycine.

4. The method according to claim 1, characterized in that In the seed treatment method, a matrix is ​​added when mixing the seeds with gibberellins, and the matrix is ​​prepared from peat soil, humus soil on the surface of the Tamarix forest, perlite and bagasse, wherein the mass ratio of peat soil, humus soil on the surface of the Tamarix forest, perlite and bagasse is 4:3:2:

1.

5. The method according to claim 1, characterized in that In the sowing treatment method, the three-layer sowing method is specifically as follows: sow the seeds mixed with the substrate at the bottom of the ditch, fill the soil with 10 cm and then sow one layer, sow three layers upwards, spray each layer with 1 / 2 concentration of modified Hoagland nutrient solution, and after the third layer is sown, sprinkle a 2-3 cm layer of substrate on the upper layer; Preferably, the seeding rate of Cistanche tubulosa is controlled at 260-300 g / 666.7 m 2 .

6. The method according to claim 1, characterized in that The method also includes a covering treatment step, wherein the covering treatment method is: in late November of the sowing year, with the Chinese Tamarix trunk as the center and expanding outward to the crown drip line, black weed-proof cloth is used to cover the surface, and the surface cover is promptly removed in late March of the following year.

7. The method according to claim 1, characterized in that It also includes the steps of irrigation, loosening the soil, weeding, pruning and disease and pest control. Among them, pruning follows the conventional principles of branch pruning, and the branches between rows are heavily pruned back and shortened to 1 / 2 to 3 / 4.

8. The method according to claim 1, characterized in that For plots close to the sea or windward, windbreaks are built around or inside the Chinese Tamarix forest in winter. The windbreaks are made of straw or mats, with a height of 100 to 120 cm and buried 30 cm deep.

9. The method according to claim 1, characterized in that For the Cistanche tubulosa that breaks through the soil in the autumn and winter of the same year, it also includes spraying exogenous calcium on its surface.

10. The method according to claim 9, characterized in that For Cistanche tubulosa that broke through the soil in autumn and winter of the same year, 15.0mmol·L -1 Spray the emerging Cistanche tubulosa with a high concentration of CaCl2 solution, with an interval of 10-15 days between the two sprays.

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