Antiviral composition containing dextran and thymol and application thereof

Abstract

The invention discloses an antiviral composition containing dextran and thymol, and particularly relates to an antiviral composition containing dextran serving as a first active component and thymol serving as a second active component, the weight ratio of the first active component to the second active component in the composition is 50: 1-1: 50, the composition can be prepared into dosage forms such as water aqua, wettable powder, soluble powder, water dispersible granules, a suspending agent, a dispersible oil suspending agent, an emulsion in water and a microemulsion. The composition is used for preventing and treating tobacco mosaic virus diseases and vegetable and fruit virus diseases, is reasonable in components, has the effects of resisting viruses and improving crop immunity, is good in antiviral effect, reduces the medication frequency and is low in medication cost, and the activity and the antiviral effect of the composition are not simple superposition of the activities of all the components but have a remarkable synergistic effect, so that the generation of resistance is retarded; the composition has good safety to crops, and meets the safety requirements of pesticide preparations. The bactericidal composition has a good control effect on tobacco virus diseases and virus diseases of vegetables, melons and fruits.

Description

Technical Field

[0001] The present invention relates to an antiviral composition containing glucomannan and thymol, and particularly to an antiviral composition for preventing and controlling tobacco mosaic virus disease and vegetable and fruit virus diseases, belonging to the technical field of pesticides. Background Art

[0002] Glucomannan, English name: Glucomannan, is composed of D-glucose and D-mannose monomers connected by β-1,4 glycosidic bonds. All glucomannans are acetylated, which makes them water-soluble.

[0003] Glucomannan can effectively inactivate viruses and has good control effects on diseases caused by various viruses. At the same time, it can also promote photosynthesis, increase the accumulation of sugars and vitamins, improve the crop's own immunity and defense response, enhance the stress resistance of crops, and effectively promote crop growth, branching, flowering, fruiting, etc. Its elicitor for inducing systemic acquired resistance in crops exhibits unique biological activities. On the one hand, it can induce crops to produce excellent broad-spectrum disease resistance, not only inhibiting the early colonization, proliferation, and expansion of virus diseases, but also inactivating the virus in vitro and inhibiting the long-distance spread of the virus. It has a high virus inhibition efficiency, good degradability, and the characteristics of no residue, safe operation, and strong stability. The control of plant diseases by glucomannan has been shown by the field efficacy tests of our company to have good control effects on tobacco, vegetable, and fruit virus diseases. However, the glucomannan has a short effective period, resulting in more frequent drug applications. Currently, glucomannan mainly exists in the form of single agents. However, continuous long-term use of glucomannan has led to certain resistance, and the single use cost is relatively high.

[0004] The common name of thymol is thymol, and its Chinese aliases are: thymol; thymol; 5-methyl-2-isopropylphenol; -isopropyl-5-methylphenol; 3-hydroxy-p-cymene. Its chemical name is 2-isopropyl-5-methylphenol (2-isopropyl-5-methyl-phenol), CAS number: 89-83-8. The domestic research on the action mechanism of active ingredients of plant essential oils such as thymol mainly focuses on antibacterial aspects. Compared with other active ingredients of plant essential oils, due to different molecular structures, the action mechanisms are different. Generally, it is considered that thymol mainly acts on the cell membrane structure, changes its permeability, causes the leakage of cell contents, and leads to the death of the bacteria. Thymol has an inhibitory effect on plant viruses and also has various biological activities, including antibacterial, antioxidant, insecticidal, and anti-cancer. At present, the specific action mechanism of thymol on plant viruses has not been fully clarified, and its antibacterial and antiviral properties make it have potential applications in plant protection.

[0005] Tobacco mosaic virus disease and vegetable and fruit virus diseases are common and frequently-occurring viral diseases, which often cause devastating disasters. At present, in the actual use process of our company, it is found that polyoxyn has a good control effect on vegetable and fruit virus diseases, but the polyoxyn has a short effective period, resulting in a large number of drug uses and high use costs. As a plant essential oil active ingredient, thymol has a high inhibitory activity against plant virus diseases, but it has not been promoted and used in the market, and the single use cost is relatively high. Summary of the Invention

[0006] The purpose of the present invention is to provide an antiviral composition with reasonable components, good antiviral effect, low drug cost and not easy to produce drug resistance.

[0007] Another purpose of the present invention is to provide the antiviral use of the above composition for preventing and treating tobacco mosaic virus disease and vegetable and fruit virus diseases.

[0008] In order to overcome the defects of existing single preparations, the technical solution of the present invention is solved as follows: The present invention contains polyoxyn as the first active ingredient and thymol as the second active ingredient, wherein the weight ratio of the first active ingredient to the second active ingredient is 50:1 to 1:50. The cumulative amount of the first active ingredient and the second active ingredient is 0.1% to 50% of the total weight of the composition, preferably 1% to 20%.

[0009] According to the methods well-known to those skilled in the art, the antiviral composition containing polyoxyn and thymol of the present invention can be formulated into any dosage form permitted in agriculture during actual application, and the preparation dosage forms are aqueous solution, wettable powder, soluble powder, water dispersible granule, suspension, dispersible oil suspension, emulsion in water, microemulsion.

[0010] For the aqueous solution dosage form, those skilled in the art are very familiar with using corresponding auxiliaries to complete the present invention. Emulsifiers such as calcium dodecylbenzenesulfonate (Agricultural Emulsion 500#), Agricultural Emulsion 700# (Common Name: Alkylphenol Formaldehyde Resin Polyoxyethylene Ether), Agricultural Emulsion 2201#, Span-60# (Common Name: Sorbitan Monostearate), Tween-60# (Common Name: Polyoxyethylene Sorbitan Monostearate), TX-10 (Common Name: Octylphenol Polyoxyethylene (10) Ether), Penetrant JFC (Fatty Alcohol Polyoxyethylene Ether), Agricultural Emulsion 1601# (Common Name: Triphenylethylphenol Polyoxypropylene Polyoxyethylene Block Polymer), Agricultural Emulsion 600#, Agricultural Emulsion 700#, Agricultural Emulsion 400#, dodecyl dimethyl benzyl ammonium chloride, one or more of them; pH regulators such as sodium carbonate, sodium bicarbonate, sodium hydroxide, potassium hydroxide, ammonia water, triethylamine, triethanolamine, one or more of them; and the water is deionized water.

[0011] For wettable powders, those skilled in the art are familiar with using corresponding adjuvants to complete the present invention. The adjuvants that can be used include: one or more of dispersants such as polycarboxylates, lignosulfonates, alkylnaphthalenesulfonates; one or more of wetting agents such as alkyl sulfates, alkyl sulfonates, naphthalenesulfonates; one or more of fillers such as diatomaceous earth, kaolin, white carbon black, light calcium carbonate, talc powder, attapulgite clay, pottery clay.

[0012] For soluble powders, those skilled in the art are familiar with using corresponding adjuvants to complete the present invention. Dispersants such as one or more of polycarboxylates, lignosulfonates, alkylnaphthalenesulfonates; wetting agents such as one or more of alkyl sulfates, alkyl sulfonates, naphthalenesulfonates; disintegrants such as one or more of soluble starch, ammonium sulfate, urea, sucrose, glucose, sodium sulfate, white carbon black.

[0013] For water dispersible granules, those skilled in the art are familiar with using corresponding adjuvants to complete the present invention. Dispersants such as one or more of polycarboxylates, lignosulfonates, alkylnaphthalenesulfonates; wetting agents such as one or more of alkyl sulfates, alkyl sulfonates, naphthalenesulfonates; disintegrants such as one or more of ammonium sulfate, urea, sucrose, glucose; binders such as one or more of diatomaceous earth, corn starch, PVA, carboxymethyl (ethyl) cellulose; fillers such as one or more of diatomaceous earth, kaolin, white carbon black, light calcium carbonate, talc powder, attapulgite clay, pottery clay.

[0014] For suspension concentrates, the adjuvants that can be used include: one or more of dispersants such as polycarboxylates, lignosulfonates, alkylnaphthalenesulfonates, TERSPERSE 2425 (produced by Huntsman Corporation, USA, alkylnaphthalenesulfonate type); one or more of emulsifiers such as Nongru 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Nongru 2201, Span - 60# (common name: sorbitan monostearate), emulsifier T - 60 (common name: polyoxyethylene sorbitan monostearate), Nongru 1601# (common name: phenethylphenol polyoxyethylene polyoxypropylene ether), TERSPERSE 4894 (produced by Huntsman Corporation, USA), TERSPERSE 2500 (produced by Huntsman Corporation, USA); one or more of wetting agents such as alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfates, alkyl sulfonates, naphthalenesulfonates, TERSPERSE 2500 (produced by Huntsman Corporation, USA); one or more of thickeners such as xanthan gum, polyvinyl alcohol, bentonite; one or more of preservatives such as formaldehyde, benzoic acid, sodium benzoate; defoamers such as silicone defoamers; antifreeze agents such as one or more of ethylene glycol, propylene glycol, glycerol, urea, inorganic salts such as sodium chloride.

[0015] For the oil-dispersible suspension concentrate, the auxiliaries that can be used are: wetting agents such as alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, lauryl alcohol polyoxyethylene ether sodium sulfate, sodium dodecylbenzenesulfonate, alkylnaphthalenesulfonate, sodium dodecyl sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate sodium sulfosuccinate, disodium alkylphenol polyoxyethylene ether sulfosuccinate, one or several of them; dispersants such as sodium lignosulfonate, calcium lignosulfonate, alkylnaphthalene formaldehyde condensate sulfonate, polycarboxylate (TERSPERSE 2700 produced by Huntsman Corporation, USA), TERSPERSE 2425 (produced by Huntsman Corporation, USA, alkylnaphthalenesulfonate type), one or several of one or more of them; emulsifiers such as calcium dodecylbenzenesulfonate, castor oil polyoxyethylene ether, alkylphenol polyoxyethylene ether formaldehyde condensate, alkylphenol polyoxyethylene polyoxypropylene ether, polyoxyethylene ether oleoyl alcoholate, alkylphenol formaldehyde resin polyoxyethylene ether, sorbitan stearate, polyoxyethylene sorbitan stearate, tribenzylethylphenol polyoxypropylene polyoxyethylene block polymer, one or several of them; thickeners such as xanthan gum, carboxymethyl cellulose, white carbon black, polyvinyl alcohol, bentonite, magnesium aluminum silicate, any one or several of them; stabilizers such as propylene oxide, tributyl phosphate, triphenyl phosphite, epoxidized soybean oil, sodium benzoate, sodium sorbate, formaldehyde, one or several of them; antifreeze agents such as ethylene glycol, propylene glycol, glycerol, urea, an inorganic salt such as sodium chloride; defoamers are one of dimethyl silicone oil defoamers, polyether-modified silicone defoamers, polyether defoamers; non-aqueous dispersion media such as soybean oil, corn oil, cottonseed oil, palm oil and linseed oil, turpentine oil, rapeseed oil, corn oil, methyl esterified vegetable oils such as methyl oleate, one or several of them.

[0016] For the emulsion in water, the auxiliaries that can be used are: emulsifiers such as nonylphenol polyoxyethylene (EO = 10) ether phosphate, tribenzylethylphenol polyoxyethylene ether phosphate, agricultural emulsion 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), agricultural emulsion 2201#, Span - 60# (common name: sorbitan stearate), Tween - 60# (common name: polyoxyethylene sorbitan stearate), TX - 10 (common name: octylphenol polyoxyethylene (10) ether), agricultural emulsion 1601 (common name: tribenzylethylphenol polyoxypropylene polyoxyethylene block polymer), agricultural emulsion 600#, agricultural emulsion 400#, one or more of them; solvents such as xylene, toluene, cyclohexanone, solvent oil (grades: S - 150, S - 180, S - 200), one or more of them; stabilizers such as triphenyl phosphite, epichlorohydrin, epoxidized soybean oil, one or more of them; antifreeze agents: ethylene glycol, propylene glycol, glycerol, urea, an inorganic salt such as sodium chloride, one or more of them; thickeners such as xanthan gum, polyvinyl alcohol, bentonite, magnesium aluminum silicate, one or more of them; preservatives such as formaldehyde, benzoic acid, sodium benzoate, one or more of them, and water is deionized water.

[0017] For microemulsions, the auxiliary agents that can be used include: emulsifiers such as calcium dodecylbenzenesulfonate (Agricultural Emulsifier 500#), Agricultural Emulsifier 700# (common name: alkylphenol formaldehyde resin polyoxyethylene ether), Agricultural Emulsifier 2201#, Span-60# (common name: sorbitan stearate), Tween-60# (common name: polyoxyethylene sorbitan stearate), TX-10 (common name: octylphenol polyoxyethylene (10) ether), Agricultural Emulsifier 1601 (common name: tribenzylethylphenol polyoxypropylene polyoxyethylene block polymer), Agricultural Emulsifier 600#, Agricultural Emulsifier 400#; co-emulsifiers such as methanol, isopropanol, n-butanol, ethanol; solvents such as cyclohexanone, N-methylpyrrolidone, xylene, toluene, solvent naphtha (grades: S-150, S-180, S-200); stabilizers such as triphenyl phosphite, epichlorohydrin; and the water is deionized water.

[0018] The components of the present invention are reasonable, having the effects of antiviral and improving the self-immunity of crops, with good antiviral effects, reducing the number of pesticide applications and the cost of using pesticides. Moreover, its activity and antiviral effect are not a simple superposition of the activities of each component, but have a significant synergistic effect, slowing down the generation of resistance, being safe for crops, meeting the safety requirements of pesticide formulations, and being suitable for the production of green foods such as vegetables. The present invention has good control effects on tobacco mosaic virus disease and vegetable and fruit virus diseases. Detailed implementation manners

[0019] The following further illustrates the content of the present invention in conjunction with embodiments.

[0020] In order to control tobacco mosaic virus disease and vegetable and fruit virus diseases in agricultural production, an indoor bioassay synergistic study on the mutual compounding of polyglucuronic acid and thymol components was carried out.

[0021] The tests were conducted using tobacco mosaic virus, watermelon mosaic virus, cucumber mosaic virus, and tomato yellow leaf curl virus (TYLCV) as the test subjects. The specific methods were as follows: 1) The thymol used in the tests was the technical material produced by Jiangxi Yuyang Chemical Co., Ltd., and the β-glucan technical material was the technical material produced by Shandong Kailinong Biotechnology Co., Ltd. The specific method was as follows: The technical materials were formulated into the required test agents. First, 5 different concentration gradients were set for the single agents and each mixed agent (set in geometric progression within the range of 5% - 90% control efficacy). Susceptible tobacco, watermelon, cucumber, and tomato varieties were selected for pot cultivation and used when the seedlings grew to the 2 - 3 leaf stage. The virus inoculation concentration was 8%, and inoculation was carried out by sap rubbing. Each treatment had no less than 3 pots, with 5 plants in each pot. 24 hours after inoculation, the agents were evenly sprayed onto the reserved tobacco, watermelon, cucumber, and tomato seedlings by the spraying method, and then cultured under suitable conditions. When the test crops were fully diseased, all the leaves of each pot of seedlings were investigated, and the following grading method was used: Grade 0: Disease-free; Grade 1: The diseased area accounts for less than 5% of the entire leaf area; Grade 3: The diseased area accounts for 6% - 15% of the entire leaf area; Grade 5: The diseased area accounts for 16% - 25% of the entire leaf area; Grade 7: The diseased area accounts for 26% - 50% of the entire leaf area; Grade 9: The diseased area accounts for more than 50% of the entire leaf area.

[0022] Calculation method: Disease index = [∑(number of diseased leaves at each level × relative level value) / (total number of investigated leaves × 9)] × 100 Control efficacy (%) = [(control disease index - treatment disease index) / control disease index] × 100 Through the linear regression analysis between the probit value of the control efficacy and the logarithm of the series of concentrations, the EC50 value of each agent was obtained, and the co-toxicity coefficient (CTC) of the mixed agent was calculated by the Sun Yunpei method to evaluate the activity of the test agents against the virus. The co-toxicity coefficient (CTC) of the compound preparation ≥ 120 showed a synergistic effect; CTC ≤ 80 showed an antagonistic effect; 80 < CTC < 120 showed an additive effect.

[0023]

[0024]

[0025]

[0026] As can be seen from Tables 1 to 4, when polyoxenose and thymol are compounded at a weight ratio of 50:1 to 1:50, they have a good synergistic effect on tobacco mosaic virus disease, watermelon mosaic virus disease, zucchini mosaic virus disease, and tomato yellow leaf curl virus disease.

[0027] Formulation Example 1: Weigh 5% polyoxenose, 0.1% thymol, 3% TX-10, 1% triethylamine, and add deionized water to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, a 5.1% polyoxenose·thymol aqueous solution is prepared.

[0028] In this example, the weight ratio of polyoxenose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 5.1%, forming a new example.

[0029] Formulation Example 2: Weigh 0.5% polyoxenose, 0.5% thymol, 3% penetrant JFC, 3% agricultural emulsifier 700#, 0.8% triethanolamine, and add deionized water to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, a 1% polyoxenose·thymol aqueous solution is prepared.

[0030] In this example, the weight ratio of polyoxenose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 1%, forming a new example.

[0031] Formulation Example 3: Weigh 1% polyoxenose, 10% thymol, 3% penetrant JFC, 2% ammonia water, and add deionized water to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, an 11% polyoxenose·thymol aqueous solution is prepared.

[0032] In this example, the weight ratio of polyoxenose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 11%, forming a new example.

[0033] Formulation Example 4: Weigh 0.5% polyoxenose, 25% thymol, 4% dodecyldimethylbenzylammonium chloride, 0.3% ammonia water, and add deionized water to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, a 25.5% polyoxenose·thymol aqueous solution is prepared.

[0034] In this example, the weight ratio of glucopolyose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 25.5%, forming a new example.

[0035] Formulation Example 5: Weigh 1% glucopolyose, 20% thymol, 1% agricultural emulsifier 600#, 3% Tween-60#, 0.4% sodium hydroxide, and add deionized water to 100% by weight. After the above raw materials are mixed and stirred until completely dissolved, a 21% glucopolyose·thymol aqueous solution is prepared.

[0036] In this example, the weight ratio of glucopolyose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 21%, forming a new example.

[0037] Formulation Example 6 Weigh 5% glucopolyose, 45% thymol, 5% calcium lignosulfonate, 2% Nekal BX (sodium dibutylnaphthalenesulfonate), 1% K-12 (sodium lauryl sulfate), 20% white carbon black, and add kaolin to 100% by weight. After the above raw materials are mixed and air-flow pulverized, a 40% glucopolyose·thymol wettable powder is prepared.

[0038] In this example, the weight ratio of glucopolyose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 50%, forming a new example.

[0039] Formulation Example 7 Weigh 10% glucopolyose, 0.5% thymol, 4% calcium lignosulfonate, 3% TERSPERSE 2700 (a polycarboxylate produced by Huntsman Corporation, USA), 2% Nekal BX (sodium dibutylnaphthalenesulfonate), 2% K-12 (sodium lauryl sulfate), 10% white carbon black, and add light calcium carbonate to 100% by weight. After the above raw materials are mixed and air-flow pulverized, a 10.5% glucopolyose·thymol wettable powder is prepared.

[0040] In this example, the weight ratio of glucopolyose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 10.5%, forming a new example.

[0041] Formulation Example 8 Weigh 0.02% glucopolyose, 0.08% thymol, 2% dispersant NNO (alkylnaphthalenesulfonate formaldehyde condensate), 1% Nekal BX (sodium dibutylnaphthalenesulfonate), 2% K-12 (sodium lauryl sulfate), 5% white carbon black, and add sodium sulfate to 100% by weight. After the above raw materials are mixed and air-flow pulverized, a 0.1% glucopolyose·thymol soluble powder is prepared.

[0042] In this embodiment, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 0.1%, forming a new embodiment.

[0043] Formulation Example 9 Weigh 0.1% glucan oligosaccharide, 2% thymol, 2% TERSPERSE 2700 (a polycarboxylate produced by Huntsman Corporation, USA), 1% K-12 (sodium dodecyl sulfate), 2% white carbon black, and ammonium sulfate to 100% by weight. The above raw materials are mixed and then micronized by air flow to obtain a 2.1% soluble powder of glucan oligosaccharide·thymol.

[0044] In this embodiment, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 2.1%, forming a new embodiment.

[0045] Formulation Example 10: Weigh 0.5% glucan oligosaccharide, 10% thymol, 3% TERSPERSE 2700 (a polycarboxylate produced by Huntsman Corporation, USA), 2% dispersant NNO (alkylnaphthalenesulfonate formaldehyde condensate), 3% Nekal BX (sodium dibutylnaphthalenesulfonate), 2% K-12 (sodium dodecyl sulfate), 3% diatomaceous earth, 5% glucose, and kaolin to 100% by weight. The above raw materials are processed by the conventional method for preparing water-dispersible granules, i.e., mixing, ultrafine air flow pulverization, mixing, and granulation steps, to obtain a 10.5% water-dispersible granule of glucan oligosaccharide·thymol.

[0046] In this embodiment, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 10.5%, forming a new embodiment.

[0047] Formulation Example 11 Weigh 1% glucan oligosaccharide, 49% thymol, 3% TERSPERSE 2700 (a polycarboxylate produced by Huntsman Corporation, USA), 3% dispersant NNO (alkylnaphthalenesulfonate formaldehyde condensate), 2% Nekal BX (sodium dibutylnaphthalenesulfonate), 1% K-12 (sodium dodecyl sulfate), 1% carboxymethyl cellulose, 3% urea, and diatomaceous earth to 100% by weight. The above raw materials are processed by the conventional method for preparing water-dispersible granules, i.e., mixing, ultrafine air flow pulverization, mixing, and granulation steps, to obtain a 50% water-dispersible granule of glucan oligosaccharide·thymol.

[0048] In this embodiment, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 80%, forming a new embodiment.

[0049] Formulation Example 12: Weigh 40% polyoxenose, 10% thymol, 4% TERSPERSE 2700 (polycarboxylate, produced by Huntsman Corporation, USA), 2% dispersant NNO (alkylnaphthalenesulfonate formaldehyde condensate), 2% Nekal BX (sodium dibutylnaphthalenesulfonate), 2% K-12 (sodium dodecyl sulfate), 3% corn starch, 5% ammonium sulfate, and attapulgite up to 100% by weight. The above raw materials are prepared into 50% polyoxenose·thymol water dispersible granules by the conventional method for preparing water dispersible granules, namely, mixing, ultrafine air flow pulverization, mixing, and granulation steps.

[0050] In this example, the weight ratio of polyoxenose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 50%, forming a new example.

[0051] Formulation Example 13: Weigh 20% polyoxenose, 20% thymol, 4% Morwet D-425 (sodium salt of alkylnaphthalenesulfonic acid condensate, produced by AkzoNobel), 3% dispersant NNO (alkylnaphthalenesulfonate formaldehyde condensate), 2% Nekal BX (sodium dibutylnaphthalenesulfonate), 2% K-12 (sodium dodecyl sulfate), 3% corn starch, 5% sucrose, and kaolin up to 100% by weight. The above raw materials are prepared into 40% polyoxenose·thymol water dispersible granules by the conventional method for preparing water dispersible granules, namely, mixing, ultrafine air flow pulverization, mixing, and granulation steps.

[0052] In this example, the weight ratio of polyoxenose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 40%, forming a new example.

[0053] Formulation Example 14: Weigh 15% polyoxenose, 15% thymol, 3% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 2% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 3% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.2% xanthan gum, 3% white carbon black, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29, produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and deionized water up to 100% by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then milled with a sand mill to obtain 30% polyoxenose·thymol suspension.

[0054] In this example, the weight ratio of polyoxenose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 30%, forming a new example.

[0055] Formulation Example 15: Weigh 10% glucopolyose, 30% thymol, 3% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 2% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 3% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.2% xanthan gum, 3% silica white, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29, produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight. The above raw materials are mixed and dispersed by high-speed shearing for 30 min, and then milled with a sand mill to obtain a 40% glucopolyose·thymol suspending agent.

[0056] In this example, the weight ratio of glucopolyose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 40%, forming a new example.

[0057] Formulation Example 16: Weigh 2% glucopolyose, 20% thymol, 3% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 1.5% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 3% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.15% xanthan gum, 5% silica white, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29, produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight. The above raw materials are mixed and dispersed by high-speed shearing for 30 min, and then milled with a sand mill to obtain a 22% glucopolyose·thymol suspending agent.

[0058] In this example, the weight ratio of glucopolyose to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 22%, forming a new example.

[0059] Formulation Example 17: Weigh 2% glucopolyose, 1% thymol, 3% TERSPERSE 4894 (produced by Huntsman Corporation, USA), 1.5% TERSPERSE 2500 (produced by Huntsman Corporation, USA), 3% TERSPERSE 2425 (produced by Huntsman Corporation, USA), 0.15% xanthan gum, 5% silica white, 5% ethylene glycol, 0.3% benzoic acid, 0.5% silicone defoamer (trade name: s-29, produced by Nanjing Sixin Applied Chemicals Co., Ltd.), and add deionized water to 100% by weight. The above raw materials are mixed and dispersed by high-speed shearing for 30 min, and then milled with a sand mill to obtain a 3% glucopolyose·thymol suspending agent.

[0060] In this embodiment, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 3%, forming a new embodiment.

[0061] Formulation Example 18 Weigh 5% glucan oligosaccharide, 0.5% thymol, 3% alkylnaphthalene sulfonate (TERSPERSE 2425) as dispersant, 2% sorbitan stearate (Span - 60#) as emulsifier, 2% polyoxyethylene sorbitan stearate (Tween - 60#) as emulsifier, 1% sodium lauryl polyoxyethylene ether sulfate as wetting agent, 2% bentonite as thickener, 5% ethylene glycol as antifreeze, 0.2% dimethyl silicone oil defoamer as defoamer, 1% epichlorohydrin as stabilizer, and add non - aqueous dispersion medium soybean oil to 100% by weight. The above raw materials are mixed, sheared and dispersed at high speed for 30 min, and then ground with a sand mill to a particle size less than 5 microns to obtain a 5.5% glucan oligosaccharide·thymol dispersible oil suspension concentrate.

[0062] In this embodiment, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 5.5%, forming a new embodiment.

[0063] Formulation Example 19 Weigh 5% glucan oligosaccharide, 25% thymol, 2% polycarboxylate (TERSPERSE 2700 produced by Huntsman Corporation, USA) as dispersant, 2% sorbitan stearate (Span - 60#) as emulsifier, 3% castor oil polyoxyethylene ether (BY - 125) as emulsifier, 1% disodium alkylphenol polyoxyethylene ether sulfosuccinate as wetting agent, 2% magnesium aluminum silicate as thickener, 4% propylene glycol as antifreeze, 0.2% polyether - modified silicone defoamer (trade name: s - 29 produced by Nanjing Sixin Applied Chemicals Co., Ltd.) as defoamer, 10% epoxidized soybean oil as stabilizer, and add non - aqueous dispersion medium methyl oleate to 100% by weight. The above raw materials are mixed, sheared and dispersed at high speed for 20 min, and then ground with a sand mill to a particle size less than 5 microns to obtain a 30% glucan oligosaccharide·thymol dispersible oil suspension concentrate.

[0064] In this embodiment, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 30%, forming a new embodiment.

[0065] Formulation Example 20 Weigh 1% glucan oligosaccharide, 5% thymol, 3% agricultural emulsifier 2201#, 2% nonylphenol polyoxyethylene (EO = 10) ether phosphate, 2% Tween - 60#, 2% Span - 60#, 1% epichlorohydrin, 5% propylene glycol, 10% xylene, 5% cyclohexanone, 0.2% xanthan gum, 0.5% benzoic acid, and add deionized water to 100% by weight. The above raw materials are mixed and emulsified by high - speed shearing to obtain 6% glucan oligosaccharide·thymol aqueous emulsion.

[0066] In this example, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 6%, forming a new example.

[0067] Formulation Example 21 Weigh 0.5% glucan oligosaccharide, 1% thymol, 3% agricultural emulsifier 2201#, 2% nonylphenol polyoxyethylene (EO = 10) ether phosphate, 2% triphenylethylphenol polyoxyethylene ether phosphate, 2% Span - 60#, 1% epichlorohydrin, 5% ethylene glycol, 20% xylene, 10% cyclohexanone, 0.2% xanthan gum, 0.5% benzoic acid, and add deionized water to 100% by weight. The above raw materials are mixed and emulsified by high - speed shearing to obtain 1.5% glucan oligosaccharide·thymol aqueous emulsion.

[0068] In this example, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 1.5%, forming a new example.

[0069] Formulation Example 22 Weigh 5% glucan oligosaccharide, 10% thymol, 4% TX - 10, 5% agricultural emulsifier 700#, 6% agricultural emulsifier 500#, 4% agricultural emulsifier 1601#, 20% cyclohexanone, 5% xylene, 5% n - butanol, 2% triphenyl phosphite. After complete dissolution and uniform mixing, add deionized water to 100% by weight, and stir to obtain 15% glucan oligosaccharide·thymol microemulsion.

[0070] In this example, the weight ratio of glucan oligosaccharide to thymol can vary between 50:1 and 1:50, and the total weight component of the two is still 15%, forming a new example.

[0071] Formulation Example 23 Weigh 1% glucan oligosaccharide, 4% thymol, 5% agricultural emulsifier 700#, 6% agricultural emulsifier 500#, 6% agricultural emulsifier 600#, 10% xylene, 10% methanol, 2% triphenyl phosphite. After complete dissolution and uniform mixing, add deionized water to 100% by weight, and stir to obtain 5% glucan oligosaccharide·thymol microemulsion.

[0072] In this embodiment, the weight ratio of glucanol to thymol can vary between 50:1 and 1:50, and the total weight content of the two is still 5%, forming a new embodiment.

[0073] Biological Example 1 Field Efficacy Test for Controlling Tobacco Virus Disease The investigation of the prevention and control of tobacco virus diseases refers to the "Guidelines for Field Efficacy Tests of Pesticides Part 73 NY / T464.73-2018 Fungicides for the Prevention and Control of Tobacco Virus Diseases". The first application of pesticides was 1 day before tobacco transplanting, and the first application of pesticides was preventive on the seedbed. After the transplanting survived, the pesticides were applied twice continuously, with an interval of 7 days. The prevention and control effect was investigated after the drug was applied. The first investigation was 10 days after the last application of pesticides, and the last investigation was before the tobacco buds appeared. A representative 5-point sampling method was randomly selected for investigation in each plot. 5 points were checked in each plot, 10 plants were checked at each point, and a total of 50 plants were checked. The investigation was graded by plant. Grading method.

[0074] Disease severity classification method: Level 0: The whole plant is disease-free; Level 1: The heart veins are open and the leaves are slightly mosaic, but the whole plant has no obvious dwarfing; Level 3: 1 / 3 of the leaves are mosaic but not deformed, or the diseased plant is dwarfed to more than 3 / 4 of the normal plant height; Level 5: 1 / 3~1 / 2 of the leaves are mosaic, or a few leaves are deformed, or the main veins turn black, or the diseased plants are dwarfed to 2 / 3~3 / 4 of the normal plant height; Level 7: 1 / 2~2 / 3 of the leaves are mosaic, or deformed or the main and lateral veins are necrotic, or the diseased plants are dwarfed to 1 / 2~2 / 3 of the normal plant height; Level 9: The leaves of the entire plant are mosaic, severely deformed or necrotic, or the diseased plant is shrunk to more than 1 / 2 of the normal plant height.

[0075] Prevention and treatment effect (%) = (CK disease index - PT disease index) / CK disease index × 100

[0076] As can be seen from Table 5, after the mixture of glucanose and thymol in a certain ratio, it has a good synergistic effect, and at a certain application dosage, it has a good control effect on tobacco virus diseases, and can significantly reduce the application amount of each active ingredient. All treatments in the experiment had no obvious adverse effects on the growth of tobacco, and the leaf color, yield, etc. were normal, and the safety was good.

[0077] Biological Example 2 Field Efficacy Test for Controlling Tomato Virus Disease The control of tomato virus disease was carried out according to the "Guidelines for Field Efficacy Tests of Pesticides Part 8 NY / T 1464.8-2007 Fungicides for Control of Tobacco Virus Disease". The plots of test agent, control agent and blank control were arranged in random blocks with a plot area of ​​30m 2, repeated 4 times, applied at the initial stage of the disease onset. Before application, the disease base number was investigated. Applied three times, with a 7-day interval between each application. The control effect was investigated before the next application and 7 - 14 days after application. The investigation adopted the random 5-point sampling method in each plot, 5 points were investigated in each plot, and 6 plants were investigated at each point. Grading method: Grade 0: No symptoms; Grade 1: Clear veins, mild mosaic; Grade 3: Mosaic on the heart leaves and middle leaves; Grade 5: Mosaic on the heart leaves and middle leaves, a few leaves are deformed, wrinkled or the plants are slightly dwarfed; Grade 7: Severe mosaic, most leaves are deformed, wrinkled or the plants are dwarfed; Grade 9: Severe mosaic, the leaves are significantly deformed, linear leaves, the plants are severely dwarfed and even die.

[0078] Drug efficacy calculation method:

[0079]

[0080] In the formula: CK 0 , CK 1 are the disease index before and after application in the blank control area respectively; PT 0 , PT 1 are the disease index before and after application in the medicament treatment area respectively.

[0081]

[0082] It can be seen from Table 6 that after mixing laminarin and thymol in a certain proportion, it has a good synergistic effect. At a certain application dose, it has a good control effect on tomato virus disease and can significantly reduce the application amount of each active ingredient. All treatments in the experiment have no obvious adverse effects on the growth of tomatoes, and the leaf color, yield, etc. are normal, and the safety is good.

[0083] To sum up, the prevention and treatment of tobacco virus disease and vegetable and fruit virus disease by the present invention has reasonable components, good antiviral effect, reduced application times, low application cost, and has a significant synergistic effect, slows down the generation of resistance, has good safety for crops, and meets the safety requirements of pesticide preparations.

Claims

1. An antiviral composition containing glucanol and thymol, characterized in that include: A) a first active ingredient, glucan; B) the second active ingredient thymol; The weight ratio of the first active ingredient to the second active ingredient is 50:1 to 1:

50.

2. The antiviral composition containing glucanol and thymol according to claim 1, characterized in that: The cumulative amount of the first active ingredient and the second active ingredient is 0.1% to 50% of the total weight of the composition.

3. The antiviral composition containing glucanol and thymol according to claim 2, characterized in that: The cumulative amount of the first active ingredient and the second active ingredient is 1% to 20% of the total weight of the composition.

4. The antiviral composition containing glucanol and thymol according to claim 1, characterized in that: When the composition is actually used, it is formulated into any dosage form permitted in agriculture.

5. The antiviral composition containing glucanol and thymol according to claim 4, characterized in that: The composition is in the form of water-based agent, wettable powder, soluble powder, water-dispersible granules, suspension, dispersible oil suspension, aqueous emulsion and microemulsion.

6. Use of the antiviral composition containing glucan and thymol according to claim 1 in preventing and treating tobacco mosaic virus disease and vegetable and fruit virus disease.

Images