Solid beverage with effects of tonifying spleen, nourishing stomach and nourishing blood vessels and preparation method thereof

Abstract

The invention discloses a solid beverage with the effects of strengthening the spleen, nourishing the stomach and maintaining blood vessels, and belongs to the technical field of beverage making. The health-care food consists of the following components: 15-20 parts of an auricularia polytricha composite extract, 8-15 parts of Chinese yam, 8-12 parts of corn kernels, 3-5 parts of soybeans, 5-10 parts of codonopsis pilosula, 5-10 parts of poria cocos, 4-6 parts of malt, 4-6 parts of peanuts, 10-15 parts of black rice, 10-15 parts of chenopodium quinoa willd, 5-10 parts of a hawthorn extract, 4-8 parts of fish collagen peptide, 3-5 parts of orange peel powder, 4-6 parts of buckwheat, 4-6 parts of inulin, 3-6 parts of blueberry fruit powder and 1-3 parts of a coix seed extract. The health-care food is prepared from the following raw materials in parts by weight: 2-4 parts of coconut granules, 1-2 parts of bitter gourd peptide, 1-2 parts of konjaku flour, 0.5-1 part of fructus momordicae powder, 0.5-1 part of corn oligopeptide powder and 2-4 parts of green tea extract. The method for jointly extracting the polypeptide and the non-dietary fiber from the auricularia polytricha is high in extraction efficiency, simple in process, short in flow, low in cost, environmentally friendly, high in safety and suitable for industrial production, and the solid beverage has the functions of tonifying the spleen, nourishing the stomach and maintaining blood vessels.

Description

Technical Field

[0001] The present invention belongs to the technical field of beverage production, and in particular, relates to a solid beverage with the effects of strengthening the spleen and nourishing the stomach and protecting blood vessels, and a production method thereof. Background Art

[0002] General solid beverages on the current market mainly exist in the form of ordinary beverages. Although some products claim to have certain health effects, they usually only focus on single or a few functions and are difficult to meet consumers' needs for comprehensive health support. Traditional production processes and technologies limit the ability to extract multiple active ingredients from a single raw material. For example, the traditional extraction process of Auricularia polytricha polysaccharide can only focus on extracting one component (such as polysaccharide), while ignoring other potential active ingredients (such as polypeptides and insoluble dietary fiber).

[0003] If this single functionality is not solved, consumers need to purchase multiple different products to meet different health needs, increasing costs and inconvenience. For example, a person who needs to strengthen the spleen and nourish the stomach and has antioxidant needs may need to buy yam powder and blueberry fruit powder at the same time, rather than a comprehensive solid beverage.

[0004] The traditional single-component extraction process does not make full use of raw materials, only extracting one active ingredient from Auricularia polytricha, causing great waste of resources. Therefore, it is urgent to research a process for compound extraction of multiple components to fill the regional gap and make reasonable and effective use of resources. Summary of the Invention

[0005] The purpose of the present invention is to provide a solid beverage with the effects of strengthening the spleen and nourishing the stomach and protecting blood vessels, and a production method thereof, so as to solve the problems raised in the background art.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A solid beverage with the effects of strengthening the spleen and nourishing the stomach and protecting blood vessels, by weight, the solid beverage is composed of the following components: 15-20 parts of Auricularia polytricha composite extract, 8-15 parts of Chinese yam, 8-12 parts of corn kernels, 3-5 parts of soybeans, 5-10 parts of Codonopsis pilosula, 5-10 parts of Poria cocos, 4-6 parts of malt, 4-6 parts of peanuts, 10-15 parts of black rice, 10-15 parts of quinoa, 5-10 parts of hawthorn extract, 4-8 parts of fish collagen peptide, 3-5 parts of orange peel powder, 4-6 parts of buckwheat, 4-6 parts of inulin, 3-6 parts of blueberry fruit powder, 1-3 parts of Coix seed extract, 2-4 parts of coconut particles, 1-2 parts of bitter melon peptide, 1-2 parts of konjac powder, 0.5-1 part of Luo Han Guo powder, 0.5-1 part of corn oligopeptide powder, and 2-4 parts of green tea extract (as an antioxidant).

[0007] A method for preparing a solid beverage with the effects of strengthening the spleen and nourishing the stomach and protecting blood vessels. Auricularia polytricha powder is dried at 60±5°C and pulverized through a 60-mesh sieve, soaked in n-hexane solution for defatting for 24 hours, dried at 50±2°C after defatting, and passed through an 80-mesh sieve for standby.

[0008] Preferably in this scheme, pure water is added according to the solid-liquid ratio of 1:50 (g / mL), ultrasonic pretreatment is carried out at an ultrasonic power of 200-400W and 35-45°C for 10 minutes, the pH value is adjusted to 7, 3% of compound enzyme (papain and neutral protease are mixed in a ratio of 1:3) is added, enzymatic hydrolysis is carried out at a constant temperature of 50±2°C for 3 hours, then the enzyme is inactivated at 90°C for 10 minutes, centrifuged, filtered, concentrated, and spray-dried to obtain Auricularia polytricha polypeptide powder.

[0009] Preferably in this scheme, weigh Auricularia auricula powder, pour it into hot water under stirring according to the liquid-solid ratio of 28:1 mL / g, place it at 70°C and continuously stir and dissolve until evenly mixed;

[0010] Stir and heat up to 90°C, maintain for 1.5-2 hours, cool down to 50°C, adjust the pH to 5.0 and stabilize for 15 minutes, then add pectinase and cellulase at the same time, carry out enzymatic hydrolysis reaction at 50°C for 3 hours, and stir from time to time;

[0011] Heat up to 85°C, maintain for 20 minutes, inactivate the enzyme activity, then cool down to 55°C, adjust the pH to 6.5 and stabilize for 15 minutes, first add 0.5% of papain with 1 million activity units, carry out enzymatic hydrolysis reaction at 50°C for 1.5 hours, and stir from time to time, then add 0.5% of neutral protease with 800,000 activity units, carry out enzymatic hydrolysis reaction at 50°C for 1.5 hours, and stir from time to time;

[0012] Heat up to 85°C, maintain for 20 minutes, inactivate the enzyme activity, then cool down to 45°C, stand overnight, cool to natural temperature, centrifuge and separate the solid and liquid the next day, retain the separated solid matter, dry and pulverize it at 80°C, add twice the volume of absolute ethanol to the liquid for alcohol precipitation, filter to obtain crude polysaccharide with a relatively high content, and dry and pulverize it at 80°C.

[0013] Preferably in this scheme, weigh the dried and constant-weight Auricularia polytricha residue, add pure water according to the liquid-solid ratio of 30:1 mL / g, adjust the pH = 6, add α-amylase (0.004 g / g), carry out enzymatic hydrolysis at a temperature of 50°C and pH = 6 for 20 min, then heat up to 95°C to inactivate the enzyme for 10 min, filter at room temperature, add 20.0 mL of 0.001 mol / L NaOH with pH = 11 to the filter residue, extract at 55°C for 2.0 h, filter and wash, collect the filter residue, and freeze-dry it to obtain IDF powder.

[0014] Preferably, the prepared Auricularia polytricha polypeptide powder, Auricularia polytricha polysaccharide, and insoluble dietary fiber (IDF) powder are mixed in a ratio of 1:3:5, pulverized, and sieved through a 60-mesh sieve.

[0015] Preferably, 15 - 20 parts of Auricularia polytricha composite extract, 8 - 15 parts of Chinese yam, 8 - 12 parts of corn kernels, 3 - 5 parts of soybeans, 5 - 10 parts of Codonopsis pilosula, 5 - 10 parts of Poria cocos, 4 - 6 parts of malt, 4 - 6 parts of peanuts, and 1 - 3 parts of Coix lacryma-jobi extract are mixed and puffed. Then, 10 - 15 parts of black rice, 10 - 15 parts of white quinoa, 5 - 10 parts of hawthorn extract, 4 - 8 parts of fish collagen peptide, 3 - 5 parts of orange peel powder, 4 - 6 parts of buckwheat, 4 - 6 parts of inulin, 3 - 6 parts of blueberry fruit powder, 2 - 4 parts of coconut granules, 1 - 2 parts of bitter melon peptide, 1 - 2 parts of konjac powder, 0.5 - 1 part of Luo Han Guo powder, and 0.5 - 1 part of corn oligopeptide powder are mixed with the puffed material, pulverized and sieved, impurities are removed, and then evenly mixed with the powdery materials that do not participate in puffing.

[0016] Preferably, in the degreasing process, ionic liquid is used to replace n-hexane, and the selection range of the ionic liquid is from [C4mim][PF6] to [C8mim][BF4].

[0017] Preferably, in the wastewater treatment stage, membrane separation technology is used to remove harmful substances in the wastewater, and the membrane pore size range is 0.01 - 0.1 microns.

[0018] Compared with the prior art, the technical effects and advantages of the present invention:

[0019] For the solid beverage with the effects of strengthening the spleen and stomach and nourishing blood vessels and its production method, general solid beverages on the existing market usually only focus on one or a few functions. However, through the scientific proportioning of multiple effective ingredients, the present invention realizes the comprehensive effects of strengthening the spleen and stomach, nourishing blood vessels, antioxidant, anti-inflammatory, etc.

[0020] Traditional single-component extraction processes often can only extract one effective ingredient. However, the present invention adopts a combined extraction method, which can simultaneously extract polypeptides, polysaccharides, and insoluble dietary fiber, make full use of raw materials, and reduce resource waste. The methods of ultrasonic-assisted enzymatic hydrolysis and hot water extraction of polysaccharides significantly improve the extraction efficiency and purity, ensuring the quality of the final product.

[0021] Using environmentally friendly ionic liquid to replace traditional n-hexane for degreasing treatment reduces environmental pollution caused by organic solvents. Using membrane separation technology to treat wastewater ensures that the wastewater discharge meets environmental protection standards and reduces the environmental pollution risk during the production process.

[0022] The addition of Chinese yam and orange peel powder helps strengthen the spleen and nourish the stomach, enhancing digestive function, and is especially suitable for people with weak spleen and stomach. The synergistic effect of polypeptides and polysaccharides further enhances this effect, contributing to the maintenance of digestive system health. Hawthorn extract contains abundant flavonoids, which helps lower cholesterol and maintain cardiovascular health. The polyphenols in green tea extract have antioxidant effects, helping to prevent blood vessel aging and arteriosclerosis. Green tea extract and blueberry fruit powder are rich in antioxidants, which can neutralize free radicals, reduce the damage of oxidative stress to cells, and delay the aging process. Polyphenols also have significant anti-inflammatory properties, helping to reduce inflammatory responses and protect the body from chronic diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the following drawings are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0024] Figure 1 Schematic diagram showing the effect of the amount of added composite enzyme on the protein hydrolysis effect of Auricularia polytricha

[0025] Figure 2 Schematic diagram showing the effect of reaction pH on the protein hydrolysis effect of Auricularia polytricha

[0026] Figure 3 Schematic diagram showing the effect of reaction time on the protein hydrolysis effect of Auricularia polytricha

[0027] Figure 4 Schematic diagram showing the effect of solid-liquid ratio on the protein hydrolysis effect of Auricularia polytricha

[0028] Figure 5 Schematic diagram showing the effect of reaction temperature on the protein hydrolysis effect of Auricularia polytricha

[0029] Figure 6 Schematic diagram showing the effect of the amount of added α-amylase on the IDF extraction rate from Auricularia polytricha residue

[0030] Figure 7 Schematic diagram showing the effect of liquid-to-solid ratio on the IDF extraction rate

[0031] Figure 8 Schematic diagram showing the effect of pH value on the IDF extraction rate

[0032] Figure 9 Schematic diagram showing the effect of extraction temperature on the IDF extraction rate

[0033] Figure 10 Schematic diagram of the influence of the extraction time of the present invention on the IDF extraction rate. Detailed implementation manners

[0034] In the following description, numerous specific details are given to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details. In other instances, some well-known technical features are not described to avoid confusion with the present invention.

[0035] Unless otherwise defined, the directions such as up, down, left, right, front, back, inside and outside involved herein are based on the up, down, left, right, front, back, inside and outside in the figures shown in the present invention, and are hereby explained together.

[0036] This embodiment provides a solid beverage having the effects of strengthening the spleen and nourishing the stomach and protecting blood vessels as Figures 1 to 10 shown. The solid beverage is composed of the following components by weight: 15-20 parts of Auricularia polytricha compound extract, 8-15 parts of Chinese yam, 8-12 parts of corn kernels, 3-5 parts of soybeans, 5-10 parts of Codonopsis pilosula, 5-10 parts of Poria cocos, 4-6 parts of malt, 4-6 parts of peanuts, 10-15 parts of black rice, 10-15 parts of white quinoa, 5-10 parts of hawthorn extract, 4-8 parts of fish collagen peptide, 3-5 parts of orange peel powder, 4-6 parts of buckwheat, 4-6 parts of inulin, 3-6 parts of blueberry fruit powder, 1-3 parts of Coix lacryma-jobi extract, 2-4 parts of coconut particles, 1-2 parts of bitter melon peptide, 1-2 parts of konjac powder, 0.5-1 part of Momordica grosvenori powder, 0.5-1 part of corn oligopeptide powder, and 2-4 parts of green tea extract (as an antioxidant).

[0037] A method for preparing a solid beverage having the effects of strengthening the spleen and nourishing the stomach and protecting blood vessels. The Auricularia polytricha powder is dried at 60±5°C and pulverized to pass through a 60-mesh sieve, soaked in n-hexane solution for defatting for 24 hours, dried at 50±2°C after defatting, and passed through an 80-mesh sieve for standby.

[0038] In this embodiment, pure water is added according to a material-liquid ratio of 1:50 (g / mL), ultrasonic pretreatment is carried out at an ultrasonic power of 200-400 W and 35-45°C for 10 minutes, the pH value is adjusted to 7, 3% of a composite enzyme (papain and neutral protease are mixed in a ratio of 1:3) is added, enzymatic hydrolysis is carried out at a constant temperature of 50±2°C for 3 hours, then the enzyme is inactivated at 90°C for 10 minutes, centrifuged, filtered, concentrated, and spray-dried to obtain Auricularia polytricha polypeptide powder.

[0039] In this embodiment, weigh the Auricularia polytricha powder, pour it into hot water under stirring according to a liquid-material ratio of 28:1 mL / g, place it at 70°C and continuously stir and dissolve until it is evenly mixed;

[0040] Stir and heat up to 90 °C, keep for 1.5 - 2 hours, cool down to 50 °C, adjust the pH to 5.0 and stabilize for 15 minutes, then add pectinase and cellulase simultaneously, and carry out enzymatic hydrolysis reaction at 50 °C for 3 hours, with stirring from time to time;

[0041] Heat up to 85 °C, maintain for 20 minutes to inactivate the enzyme activity, then cool down to 55 °C, adjust the pH to 6.5 and stabilize for 15 minutes, first add 0.5% of papain with 1 million activity units, carry out enzymatic hydrolysis reaction at 50 °C for 1.5 hours, with stirring from time to time, then add 0.5% of neutral protease with 800,000 activity units, and carry out enzymatic hydrolysis reaction at 50 °C for 1.5 hours, with stirring from time to time;

[0042] Heat up to 85 °C, maintain for 20 minutes to inactivate the enzyme activity, then cool down to 45 °C, let it stand overnight, cool to the natural temperature, centrifuge and separate the next day, carry out solid - liquid separation, keep the separated solid matter, dry and crush it at 80 °C, add twice the volume of absolute ethanol to the liquid for alcohol precipitation, filter to obtain crude polysaccharide with a relatively high content, and place it at 80 °C for drying and crushing.

[0043] In this example, weigh the residue of Auricularia polytricha with constant weight after drying, add pure water according to the liquid - to - solid ratio of 30:1 mL / g, adjust the pH = 6, add α - amylase (0.004 g / g), carry out enzymatic hydrolysis at a temperature of 50 °C and pH = 6 for 20 min, then heat up to 95 °C to inactivate the enzyme for 10 min, filter at room temperature, add 20.0 mL of 0.001 mol / L NaOH with pH = 11 to the filter residue, extract at 55 °C for 2.0 h, filter and wash, collect the filter residue and freeze - dry it to obtain IDF powder.

[0044] In this example, mix the prepared Auricularia polytricha polypeptide powder, Auricularia polytricha polysaccharide, and insoluble dietary fiber (IDF) powder in a ratio of 1:3:5, crush, and sieve through 60 - mesh.

[0045] In this example, mix 15 - 20 parts of Auricularia polytricha composite extract, 8 - 15 parts of Chinese yam, 8 - 12 parts of corn kernels, 3 - 5 parts of soybeans, 5 - 10 parts of Codonopsis pilosula, 5 - 10 parts of Poria cocos, 4 - 6 parts of malt, 4 - 6 parts of peanuts, and 1 - 3 parts of Coix lacryma - jobi extract for puffing. Then mix 10 - 15 parts of black rice, 10 - 15 parts of white quinoa, 5 - 10 parts of hawthorn extract, 4 - 8 parts of fish collagen peptide, 3 - 5 parts of orange peel powder, 4 - 6 parts of buckwheat, 4 - 6 parts of inulin, 3 - 6 parts of blueberry fruit powder, 2 - 4 parts of coconut granules, 1 - 2 parts of bitter melon peptide, 1 - 2 parts of konjac powder, 0.5 - 1 part of Luo Han Guo powder, and 0.5 - 1 part of corn oligopeptide powder with the puffed material, crush and sieve, clean the impurities, and mix evenly with the non - puffed powdery materials.

[0046] In this embodiment, an ionic liquid is used instead of n-hexane during the degreasing process, and the selection range of the ionic liquid is from [C4mim][PF6] to [C8mim][BF4].

[0047] In this embodiment, a membrane separation technology is adopted in the wastewater treatment stage to remove harmful substances in the wastewater, and the membrane pore size ranges from 0.01 to 0.1 microns.

[0048] In this embodiment, Auricularia Polyricha Sacc, also known as Auricularia polytricha (Mont.) Sacc. var. pubescens Pilát or Auricularia polytricha (Mont.) Sacc. var. hypoleuca (Berk.) Henn., grows in tropical and subtropical regions. It is a common large edible mushroom with both medicinal and edible properties in China and has relatively high medicinal efficacy. It contains nutrients such as polysaccharides, proteins, amino acids, polyphenols, vitamins, dietary fiber, and mineral elements.

[0049] In this embodiment, plant polypeptides are important low-molecular substances in organisms. They can serve as the precursors of RNA and DNA and can also participate in the body's metabolism as regulatory substances in biochemical processes. Auricularia Polyricha Sacc polypeptides can, to a certain extent, reduce the levels of cholesterol and triglycerides in the blood, reduce the deposition of lipids on the blood vessel walls, thereby reducing the risk of atherosclerosis, and can inhibit platelet aggregation and prevent blood clots from blocking blood vessels.

[0050] In this embodiment, there are types such as lignin, cellulose, and hemicellulose in insoluble dietary fiber (IDF). The human small intestine cannot effectively absorb it. The insoluble dietary fiber extracted from Auricularia Polyricha Sacc can promote gastrointestinal peristalsis, help digestion, prevent constipation, thereby reducing the burden on the spleen and stomach, and can adsorb impurities in the intestine, making food residues and the like more smoothly discharged from the body.

[0051] In this embodiment, Codonopsis pilosula + Poria cocos + Dioscorea opposita form a core combination for strengthening the spleen and replenishing qi. Codonopsis pilosula is a classic medicinal herb for invigorating qi and strengthening the spleen, which can enhance digestive function and improve anorexia. Poria cocos is good at strengthening the spleen and promoting diuresis to eliminate dampness, and promoting the spleen and stomach to transform and transport water and grain essence. Dioscorea opposita powder can tonify the spleen and stomach, promote the production of body fluid and moisten the lungs, and has an improvement effect on chronic diarrhea and indigestion. The three form a "qi-invigorating - spleen-strengthening - root-consolidating" golden triangle structure.

[0052] In this embodiment, Coix lacryma-jobi + Hordeum vulgare L. var. germinatum + Crataegus pinnatifida + Citrus reticulata Blanco form an auxiliary compatibility for dispelling dampness and promoting digestion. Coix lacryma-jobi can promote diuresis and percolate dampness, and resolve spleen deficiency with dampness obstruction. Hordeum vulgare L. var. germinatum can promote digestion and regulate the middle energizer, and is especially good at resolving food stagnation of rice and flour. Crataegus pinnatifida can strengthen the stomach and promote digestion. Together with Citrus reticulata Blanco (dried tangerine peel), it can regulate qi and resolve phlegm, and improve abdominal distension and excessive phlegm.

[0053] In this embodiment, Auricularia Polyricha Sacc powder + Amorphophallus konjac powder + inulin dietary fiber have synergistic effects. Auricularia Polyricha Sacc contains crude fiber to promote intestinal peristalsis, and together with the glucomannan of Amorphophallus konjac powder, it forms a prebiotic environment. Inulin, as a soluble dietary fiber, can regulate the balance of intestinal flora and complement the dampness-dispelling function of Poria cocos.

[0054] In this embodiment, the combination of grains and miscellaneous grains: grains such as soybeans, peanuts, corn, black rice flour, quinoa, and buckwheat provide nutritional substrates for supplementation: plant protein (repairing gastric mucosa), B vitamins (promoting the synthesis of digestive enzymes), and complex carbohydrates (gently nourishing the spleen and stomach). This is based on the traditional Chinese medicine concept of "grains are for nourishment" for spleen and stomach nourishment. The special functional ingredient peptide strengthening combination: fish collagen peptide: repairs gastric mucosa damage; bitter gourd peptide + corn oligopeptide: regulates intestinal flora and improves metabolism; Luo Han Guo powder: a natural sweetener replaces sucrose to avoid greasiness and hinderance to the stomach.

[0055] In this embodiment, the principle of the formula for vascular maintenance:

[0056] The core combination for blood lipid metabolism regulation:

[0057] 1. Auricularia polytricha powder: contains auricularia polysaccharide and dietary fiber, can inhibit platelet aggregation and reduce blood viscosity, and its anticoagulant effect is close to that of aspirin but without side effects;

[0058] 2. Buckwheat flour / hawthorn: rich in rutin and hawthorn flavonoids, reduces cholesterol synthesis by inhibiting the activity of HMG-CoA reductase;

[0059] 3. Fish collagen peptide: promotes the production of apolipoprotein A1 and increases the level of high-density lipoprotein.

[0060] The synergistic mechanism for blood pressure regulation:

[0061] 1. Poria cocos polysaccharide: regulates the activity of sodium-potassium pump and promotes sodium ion excretion;

[0062] 2. Corn oligopeptide: inhibits the activity of angiotensin-converting enzyme (ACE);

[0063] 3. Codonopsis pilosula saponin: enhances myocardial contractility while dilating peripheral blood vessels.

[0064] The vascular protection system:

[0065] 1. Blueberry anthocyanin: repairs oxidative damage of vascular endothelium and improves the bioavailability of nitric oxide;

[0066] 2. Bitter gourd peptide: activates the AMPK pathway to inhibit abnormal proliferation of vascular smooth muscle;

[0067] 3. Inulin: metabolizes to produce short-chain fatty acids and inhibits the process of vascular calcification.

[0068] The blood glucose management network:

[0069] 1. Konjac glucomannan: forms an intestinal gel barrier to delay sugar absorption;

[0070] 2. Quinoa protein: enhances the sensitivity of insulin receptors;

[0071] 3. Mogroside: a zero-calorie sweetener that replaces sucrose.

[0072] Synergistic design:

[0073] 1. Coix seeds + Poria: a classic diuretic and dampness-inducing drug pair, improving water-sodium retention type hypertension;

[0074] 2. Hawthorn + malt: promote enzymatic synergy of lipid decomposition;

[0075] 3. Yam + soybean: phytoestrogens and soy isoflavones combine to provide antioxidant effects.

[0076] The solid beverage in this embodiment is formulated and combined with natural ingredients such as Auricularia auricula composite extract (containing Auricularia auricula polypeptides, Auricularia auricula polysaccharides, and non-dietary fiber), yam, hawthorn, Codonopsis pilosula, Poria cocos, Coix seeds, malt, tangerine peel, etc. according to the principle of monarch, minister, assistant and envoy compatibility, which effectively exerts the effects of various ingredients, is conducive to the synergistic effect between the ingredients, has the functions of strengthening the spleen and stomach and maintaining blood vessels, and the prepared solid beverage has a simple preparation process, and the product is easy to store and convenient to use.

[0077] In this embodiment, please refer to Figures 1 - 10 As shown in the figure, the effect of the amount of added complex enzyme on the hydrolysis effect of Auricularia auricularia protein was determined by adding 2%, 3%, 4%, 5%, and 6% of the complex enzyme. Figure 1 As shown in the figure, it can be found that with the increase of the amount of compound enzyme added, the hydrolysis degree of Auricularia auriculariae protein will first increase. When the amount of compound enzyme added reaches 3%, the protein hydrolysis degree reaches the maximum value. After adding more than 3%, the hydrolysis effect will deteriorate. The main reason is that when the amount of enzyme added is 3%, the action sites of the enzyme and Auricularia auriculariae have reached the saturation point

[13] . If compound enzyme is added to it, it is equivalent to increasing the amount of solvent. The concentration of polypeptides produced by the hydrolysis of Auricularia auriculariae will become smaller and smaller, and the chance of contact between the enzyme and Auricularia auriculariae will decrease. Therefore, continuing to add enzyme will not increase the hydrolysis degree. Therefore, 3% is the optimal amount of compound enzyme added.

[0078] In this embodiment, Figure 2 To determine the effect of reaction pH on the hydrolysis of Auricularia auricularia protein, the effects of reaction pH values ​​of 6.5, 7.0, 7.5, 8.0, and 8.5 on the hydrolysis of Auricularia auricularia protein were determined. Figure 2 As shown. It was found that the hydrolysis degree of Auricularia auricularia protein first increased with the increase of reaction pH. When the pH increased to 7.5, the protein hydrolysis effect was the best. After the pH was greater than 7.5, the hydrolysis degree would not continue to increase, but would gradually decrease. This is because when the pH continued to increase, the excessively high pH would cause the enzyme to be inactivated, thereby reducing the enzymatic hydrolysis rate. Therefore, the optimal pH for the reaction is 7.5.

[0079] In this example, the effects of reaction times of 1 h, 2 h, 3 h, 4 h, and 5 h on the hydrolysis of Auricularia polytricha protein were determined, as Figure 3 shown. As the reaction time continued, the degree of hydrolysis also increased. However, when the reaction time exceeded 3 h, the hydrolysis degree of Auricularia polytricha protein remained basically unchanged. Due to the presence of the enzyme, macromolecular proteins would be hydrolyzed into polypeptides

[14] . With a certain amount of substrate, the reaction reached the saturation point at 3 h. As the time continued to increase, the hydrolysis degree would not change much. Therefore, 3 h is the optimal enzymatic hydrolysis time.

[0080] In this example, the effects of solid-liquid ratios of 1:30 g / mL, 1:40 g / mL, 1:50 g / mL, 1:60 g / mL, and 1:70 g / mL on the hydrolysis of Auricularia polytricha protein were determined, as Figure 4 shown. The more distilled water was added, the greater the hydrolysis degree of Auricularia polytricha protein. However, when the solid-liquid ratio reached 1:50 g / mL, the protein hydrolysis degree decreased continuously. The main reasons are as follows: When less solvent was added, the solution was distributed with a high concentration of protein, resulting in slow water flow [9], the movement of the enzyme was restricted, and the chance of the enzyme contacting Auricularia polytricha decreased, and finally the hydrolysis degree would also decrease; while if the concentration was too low, the possibility of protein and substrate collision would decrease, and the hydrolysis effect of Auricularia polytricha protein was not good

[15] . Therefore, 1:50 g / mL is the optimal solid-liquid ratio.

[0081] In this example, the effects of reaction temperatures of 40 °C, 45 °C, 50 °C, 55 °C, and 60 °C on the hydrolysis of Auricularia polytricha protein were determined, as Figure 5 shown. As the reaction temperature increased, the hydrolysis degree of Auricularia polytricha protein also increased. However, when the temperature exceeded 50 °C, the protein hydrolysis effect gradually became worse. Because in a low-temperature environment, the enzyme activity was not high, resulting in a poor hydrolysis effect; while high temperature would cause the enzyme to denature and inactivate. Therefore, the optimal reaction temperature is 50 °C.

[0082] In this example, the effects of different amounts of α-amylase on the extraction rate of IDF were analyzed, as Figure 6 shown. A single-factor experiment on the extraction of insoluble dietary fiber was carried out for the amount of added α-amylase. From the trend in the figure, among the extraction results of the five added amounts of α-amylase, the trend graph is in the shape of a small mountain peak. When the amount of added α-amylase was 0.004 g / g, the extraction rate of IDF reached the highest. Therefore, from the above experimental analysis, the optimal amount of α-amylase added for extracting insoluble dietary fiber from Auricularia polytricha residue should be 0.004 g / g.

[0083] In this example, the effects of different liquid-to-material ratios on the extraction rate of IDF were analyzed, as Figure 7As shown, a single-factor experiment on the extraction of insoluble dietary fiber was conducted for the extracted solid-to-liquid ratio. From the trend in the figure, it can be seen that for the extraction results under five solid-to-liquid ratio conditions, the trend graph is in the shape of a small mountain peak. When the solid-to-liquid ratio is 30:1 mL / g, the extraction rate of IDF reaches the peak. Therefore, from the above experimental analysis, the optimal solid-to-liquid ratio for extracting insoluble dietary fiber from Auricularia polytricha residues should be 30:1 mL / g.

[0084] In this embodiment, the effect of different pH values on the extraction rate of IDF was analyzed. As Figure 8 shown, a single-factor experiment on the extraction of insoluble dietary fiber was conducted for the pH value. From the trend in the figure, it can be seen that for the extraction results under five pH value conditions, the trend graph is in the shape of a small mountain peak. When the pH value is 11, the extraction rate of IDF reaches the peak. Therefore, from the above experimental analysis, the optimal pH value for extracting insoluble dietary fiber from Auricularia polytricha residues should be 11.

[0085] In this embodiment, the effect of different extraction temperatures on the extraction rate of IDF was analyzed. As Figure 9 shown, a single-factor experiment on the extraction of insoluble dietary fiber was conducted for the extraction temperature. From the trend in the figure, it can be seen that for the extraction results under five extraction temperature conditions, the trend graph is in the shape of a small mountain peak. When the extraction temperature is 55 °C, the extraction rate of IDF reaches the peak. Therefore, from the above experimental analysis, the optimal extraction temperature for extracting insoluble dietary fiber from Auricularia polytricha residues should be 55 °C.

[0086] In this embodiment, the effect of different extraction times on the extraction rate of IDF was analyzed. As Figure 10 shown, a single-factor experiment on the extraction of insoluble dietary fiber was conducted for the extraction time. From the trend in the figure, it can be seen that for the extraction results under five extraction time conditions, the trend graph is in the shape of a small mountain peak. When the extraction time is 2.0 h, the extraction rate of IDF reaches the peak. Therefore, from the above experimental analysis, the optimal extraction time for extracting insoluble dietary fiber from Auricularia polytricha residues should be 2.0 h.

[0087] It should be noted that in this text, relational terms such as "one" and "two" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements but also includes other elements not expressly listed, or further includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising a..." does not exclude the presence of additional identical elements in the process, method, article or device comprising said element.

[0088] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A solid beverage having the effects of strengthening the spleen and stomach and maintaining blood vessels, characterized in that: The solid beverage consists of the following ingredients by weight: 15-20 parts of Auricularia auricula composite extract, 8-15 parts of yam, 8-12 parts of corn kernels, 3-5 parts of soybeans, 5-10 parts of Codonopsis pilosula, 5-10 parts of Poria cocos, 4-6 parts of malt, 4-6 parts of peanuts, 10-15 parts of black rice, 10-15 parts of quinoa, 5-10 parts of hawthorn extract, 4-8 parts of fish collagen peptides, 3-5 parts of orange peel powder, 4-6 parts of buckwheat, 4-6 parts of inulin, 3-6 parts of blueberry powder, 1-3 parts of coix seed extract, 2-4 parts of coconut kernels, 1-2 parts of bitter melon peptide, 1-2 parts of konjac powder, 0.5-1 parts of monk fruit powder, 0.5-1 parts of corn oligopeptide powder, and 2-4 parts of green tea extract.

2. A method for preparing the solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels as claimed in claim 1, characterized in that: The Auricularia auricula powder was dried at 60±5°C and crushed to pass through a 60-mesh sieve, and then soaked and defatted in a n-hexane solution for 24 hours. The defatted Auricularia auricula powder was dried at 50±2°C and passed through an 80-mesh sieve for later use.

3. The method for preparing a solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels according to claim 2, characterized in that: Purified water was added at a material-liquid ratio of 1:50 (g / mL), ultrasonic pretreatment was performed at an ultrasonic power of 200-400 W and 35-45° C. for 10 minutes, the pH value was adjusted to 7, 3% of the composite enzyme was added, enzymolysis was performed at a constant temperature of 50±2° C. for 3 hours, and then the enzyme was inactivated at 90° C. for 10 minutes, centrifuged, filtered, concentrated, and spray-dried to obtain Auricularia auriculariae polypeptide powder.

4. The method for preparing a solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels according to claim 3, characterized in that: Weigh the fungus powder, pour it into hot water with stirring at a liquid-to-solid ratio of 28:1 mL / g, place it at 70°C and stir it continuously to dissolve until it is evenly mixed; Stir and heat to 90°C, maintain for 1.5-2 hours, cool to 50°C, adjust pH to 5.0 and stabilize for 15 minutes, then add pectinase and cellulase at the same time, and perform enzymolysis at 50°C for 3 hours with occasional stirring; Raise the temperature to 85°C, maintain for 20 minutes to inactivate the enzyme, then cool to 55°C, adjust the pH to 6.5 and stabilize for 15 minutes, first add 0.5% of 1 million units of papain, and perform enzymatic reaction at 50°C for 1.5 hours with occasional stirring, then add 0.5% of 800,000 units of neutral protease, and perform enzymatic reaction at 50°C for 1.5 hours with occasional stirring; The temperature was raised to 85°C and maintained for 20 minutes to inactivate the enzyme, then the temperature was lowered to 45°C, allowed to stand overnight, cooled to natural temperature, and centrifuged the next day for solid-liquid separation. The separated solids were retained, dried at 80°C and crushed, and two times the volume of anhydrous ethanol was added to the liquid for alcohol precipitation, filtered to obtain crude polysaccharides with a relatively high content, which were dried at 80°C and crushed.

5. The method for preparing a solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels according to claim 4, characterized in that: Weigh the dried Auricularia auricularia residue of constant weight, add pure water according to the liquid-to-solid ratio of 30:1 mL / g, adjust the pH to 6, add α-amylase, hydrolyze at 50°C and pH = 6 for 20 minutes, then heat to 95°C to inactivate the enzyme for 10 minutes, filter at room temperature, add 20.0 mL of 0.001 mol / L NaOH with a pH of 11 to the residue, extract at 55°C for 2.0 h, filter, wash, collect the residue, and freeze-dry to obtain IDF powder.

6. The method for preparing a solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels according to claim 5, characterized in that: The Auricularia auricularia polypeptide powder, Auricularia auricularia polysaccharide and water-insoluble dietary fiber powder prepared above were mixed in a ratio of 1:3:5, crushed and sieved through 60 meshes.

7. The method for preparing a solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels according to claim 6, characterized in that: 15-20 parts of Auricularia auricula composite extract, 8-15 parts of yam, 8-12 parts of corn kernels, 3-5 parts of soybeans, 5-10 parts of Codonopsis pilosula, 5-10 parts of Poria cocos, 4-6 parts of malt, 4-6 parts of peanuts and 1-3 parts of Coix seed extract are mixed and puffed; 10-15 parts of black rice, 10-15 parts of quinoa, 5-10 parts of hawthorn extract, 4-8 parts of fish collagen peptide, 3-5 parts of orange peel powder, 4-6 parts of buckwheat, 4-6 parts of inulin, 3-6 parts of blueberry powder, 2-4 parts of coconut grains, 1-2 parts of bitter melon peptide, 1-2 parts of konjac powder, 0.5-1 parts of monk fruit powder and 0.5-1 parts of corn oligopeptide powder are mixed with the puffed materials, then crushed and sieved to remove impurities and evenly mixed with the powdered materials not involved in the puffing.

8. The method for preparing a solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels according to claim 7, characterized in that: Ionic liquids are used instead of n-hexane in the degreasing process, and the selection range of ionic liquids is [C4mim][PF6] to [C8mim][BF4].

9. The method for preparing a solid beverage having the effects of strengthening the spleen and stomach and protecting blood vessels according to claim 8, characterized in that: Membrane separation technology is used in the wastewater treatment stage to remove harmful substances in the wastewater, and the membrane pore size ranges from 0.01-0.1 microns.

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