Composition for brightening skin color, application of composition, makeup removing cream and preparation method of makeup removing cream

Abstract

The invention provides a skin brightening composition and application thereof, makeup removing cream and a preparation method thereof, and relates to the technical field of skin care products. The skin color brightening composition provided by the invention is prepared from a skin conditioner A, polyglycerol-10-laurate, thiotaurine and a skin conditioner B, the camellia extract in the skin conditioner B has the antioxidant characteristics of removing active oxygen and enhancing antioxidant enzyme; the polyglycerol-10-laurate can be used for remarkably inhibiting saccharification reaction, so that protein in the skin is prevented from being damaged; thiotaurine is attached to damaged carbonylated protein to mark protein, so that the thiotaurine is recognized by proteasome and is subjected to enzymolysis to form small peptide fragments, and the small peptide fragments can be further degraded by other enzymes in cell cytoplasm; by utilizing the characteristic that the makeup removing cream is high in oil content, the skin surface lipid peroxidation rate can be reduced through oil-soluble oil and water flushing cleaning steps in the cleaning process; the polyquaternium-51 in the skin conditioner B can wrap the three components, and is attached to the skin to play a role in the cleaning process by utilizing the electrostatic interaction, so that the skin is prevented from being washed away.

Description

Technical Field

[0001] This application relates to the technical field of skin care products, and particularly relates to a skin brightening composition and its application, a cleansing balm and its preparation method. Background Art

[0002] With the development of the market, consumers' skin care needs have become more refined, shifting from traditional anti-aging and moisturizing to more specific skin brightening. At the same time, with the accelerating pace of life, external pressure, environmental factors, physical conditions, and bad living habits are more likely to promote sallow skin complexion. For Eastern beauty-loving women who pursue fair and moist skin, how to solve the problem of dull skin has become a trouble that must be faced.

[0003] The main function of existing cleansing balms is to clean the skin, remove makeup residues, dirt, and excess sebum, rather than brighten the skin complexion. Although some cleansing balms may add moisturizing and antioxidant ingredients, due to the short residence time on the skin, compared with leave-on products such as essence and cream, it is difficult to achieve a significant brightening effect. Summary of the Invention

[0004] The purpose of this application is to provide a skin brightening composition and its application, a cleansing balm and its preparation method, aiming to solve the problem that existing cleansing balms are difficult to achieve a skin brightening effect.

[0005] To achieve the above purpose, this application provides a skin brightening composition, which includes, by mass percentage:

[0006]

[0007] In some embodiments, it includes, by mass percentage:

[0008]

[0009] In some embodiments, it includes, by mass percentage:

[0010]

[0011] In some embodiments, it includes, by mass percentage:

[0012]

[0013] In some embodiments, the skin conditioner A, by weight parts, includes the following components: 45 - 50 parts of 1,3-propanediol, 45 - 54 parts of water, and 1 - 5 parts of camellia extract.

[0014] In some embodiments, the skin conditioner B is in parts by weight and includes the following components: 90 - 94 parts of water, 1 - 5 parts of polyquaternium - 51, and 1 - 9 parts of phenoxyethanol.

[0015] The present application also provides the use of the above - mentioned skin - brightening composition in the preparation of skin care products.

[0016] The present application also provides a cleansing balm, which includes the above - mentioned skin - brightening composition.

[0017] In some embodiments, by mass percentage, the cleansing balm further includes: 45% - 60% of a solvent, 5% - 25% of an emulsifier, and 3% - 8% of an oil - phase thickener.

[0018] In some embodiments, by mass percentage, the cleansing balm includes:

[0019]

[0020] The present application also provides a preparation method of the above - mentioned cleansing balm, which includes:

[0021] Adding isocetane, coco - caprylate / caprate, PEG - 20 glyceryl triisostearate, and synthetic wax into a reaction vessel, heating and stirring to obtain phase A;

[0022] After cooling phase A, adding the remaining components in sequence and stirring to obtain the cleansing balm.

[0023] In some embodiments, the heating temperature is 92 - 95 °C, and the cooling temperature is 80 - 85 °C.

[0024] Compared with the prior art, the beneficial effects of the present application include:

[0025] The skin - brightening composition provided by the present application includes skin conditioner A, polyglyceryl - 10 - laurate, thiotaurine, and skin conditioner B. The camellia extract in skin conditioner A has antioxidant properties of scavenging reactive oxygen species and enhancing antioxidant enzymes; polyglyceryl - 10 - laurate can significantly inhibit the glycation reaction, thereby protecting proteins in the skin from damage; thiotaurine attaches to damaged carbonylated proteins to label the proteins, making them recognizable by the proteasome. The enzymatic components of the proteasome shear the proteins into small peptide fragments, enabling them to be further degraded by other enzymes in the cell cytoplasm; polyquaternium - 51 in skin conditioner B is a living - body - affinity polymer with phosphorylcholine groups. It can encapsulate the above three components and, by electrostatic action, attach to the skin during the cleaning process and play a role without being washed away.

[0026] The cleansing balm provided by this application adopts the above-mentioned skin brightening composition. Starting from the skin mechanism that causes dull skin color and combining with the characteristics of the cleansing balm category, it brightens the skin color comprehensively from four dimensions, including reducing free radicals on the skin surface, inhibiting the synthesis of advanced glycation end products (AGES), removing the sources of skin dullness, namely carbonyl proteins and lipid peroxides, and reducing the accumulation of old cutin on the skin surface. It solves the problem that the reduction effect is not obvious in a single aspect. Through the synergistic effect of the four aspects, it can more effectively solve skin dullness and brighten the skin color. Detailed implementation manners

[0027] As used herein, the terms:

[0028] "Prepared from" is synonymous with "comprising". As used herein, the terms "comprising", "including", "having", "containing" or any other variation thereof are intended to cover non-exclusive inclusion. For example, a composition, step, method, article or device containing the listed elements does not have to be limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, article or device.

[0029] The connecting word "consisting of" excludes any unstated element, step or component. If used in a claim, this phrase will render the claim closed, excluding materials other than those described, except for conventional impurities associated therewith. When the phrase "consisting of" appears in a clause of the claim body rather than immediately following the subject, it only limits the elements described in that clause; other elements are not excluded from the claim as a whole.

[0030] When an equivalent, concentration, or other value or parameter is expressed as a range, a preferred range, or a range defined by a series of upper preferred values and lower preferred values, this should be understood to specifically disclose all ranges formed by any pairing of any range upper limit or preferred value with any range lower limit or preferred value, regardless of whether the range is disclosed individually. For example, when the range "1 - 5" is disclosed, the described range should be interpreted to include the ranges "1 - 4", "1 - 3", "1 - 2", "1 - 2 and 4 - 5", "1 - 3 and 5", etc. When a numerical range is described herein, unless otherwise specified, the range is intended to include its end values and all integers and fractions within the range.

[0031] In these examples, unless otherwise specified, the parts and percentages are by mass.

[0032] "Parts by mass" refers to the basic measurement unit representing the mass ratio relationship of multiple components. One part can represent any unit mass, such as 1 g or 2.689 g, etc. If we say that the mass of component A is a parts by mass and the mass of component B is b parts by mass, it means the mass ratio of component A to component B is a:b. Or it means the mass of component A is aK and the mass of component B is bK (K is an arbitrary number representing a multiple factor). It should not be misunderstood that, different from the mass fraction, the sum of the mass parts of all components is not limited to 100 parts.

[0033] For skin dullness and skin darkness, their mechanisms are different. Skin darkness is related to melanin in the skin: Melanin is produced by melanosomes in melanocytes. According to skin physiology, the main factors determining skin color depth are the content and distribution of skin melanin. Melanocytes are located in the basal layer of the skin. The secretion of melanin by melanocytes is an extremely complex biological process, involving mechanisms such as the generation, dendrite formation, maturation, and transport of melanosomes, and is closely related to keratinocytes and the extracellular matrix, etc.

[0034] The reasons for skin dullness include: oxidation, glycation, carbonylation, and the accumulation of oxidized lipids. Carbonylation and the accumulation of oxidized lipids are the main culprits causing skin dullness. Reactive Carbonyl Species (RCS) are some active aldehyde compounds containing unsaturated chemical bonds. RCS play a very important role in our human body. In the process of oxidants and free radicals attacking the human body and causing various damage mechanisms, RCS is the direct factor, and the main target of carbonylation is proteins.

[0035] Proteins react with the reactive carbonyl species RCS under non-enzymatic conditions and are modified by irreversible carbonyl groups. At the same time, under oxidative stress, they are carbonylated by oxygen to form carbonylated proteins. After protein carbonylation, the shape and function of the protein itself are changed, and the original function of the protein in the human body is lost. Carbonylated proteins play a very important role in the mechanism of human skin dullness. The accumulation of carbonylated proteins and lipid peroxides in the stratum corneum causing skin dullness is the most important. Simply inhibiting glycation and oxidation reactions cannot solve the problem at the root. Protein carbonylation and lipid peroxidation are irreversible modifications, so there is only degradation and accumulation.

[0036] This application provides a skin brightening composition, which includes, by mass percentage:

[0037]

[0038] Wherein, the skin conditioner A comprises the following components by weight: 45-50 parts of 1,3-propylene glycol, 45-54 parts of water, and 1-5 parts of camellia extract, wherein the active ingredient is camellia extract, which has a good ability to scavenge free radicals. The camellia extract increases the protein expression and activity of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxidase in cells. Camellia extract has the antioxidant properties of scavenging active oxygen and enhancing antioxidant enzymes. Skin conditioner A can be, for example, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5% or any value between 0.05% and 0.5%.

[0039] Among them, the main function of polyglycerol-10-laurate is to resist sugar. Glucose undergoes a glycation reaction with proteins in the body to produce AGEs (advanced glycation end products) - brown, inferior proteins. AGEs not only damage the key components in the skin structure - collagen and its supporting structure elastin, causing the skin to lose its firmness, become loose and droopy, but also cause the skin to become dark yellow. Polyglycerol-10-laurate can significantly inhibit the glycation reaction, thereby protecting the proteins in the skin from damage. Polyglycerol-10-laurate can, for example, be 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5% or any value between 0.05% and 0.5%.

[0040] Among them, the main function of thiotaurine is to resist carbonyl groups. Thiotaurine attaches to damaged carbonylated proteins and marks the proteins so that they can be recognized by proteasomes. The enzymatic components of proteasomes cleave proteins into small peptide fragments, which can be further degraded by other enzymes in the cell cytoplasm. The makeup remover cream emulsifies after meeting water, forming hydrophilic micelles to wrap hydrophobic dirt, and takes away oxidation products (such as formed lipid peroxides) during rinsing, directly reducing oxidation products. Thiotaurine can be, for example, 0.05%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.35%, 0.4%, 0.45%, 0.5% or any value between 0.05% and 0.5%.

[0041] Among them, the skin conditioner B is in parts by weight and includes the following components: 90 - 94 parts of water, 1 - 5 parts of polyquaternium - 51, and 1 - 9 parts of phenoxyethanol. Among them, the active ingredient is polyquaternium - 51, which is a living body - affinity polymer with phosphorylcholine (PC) groups. It can encapsulate the above three components and, by means of electrostatic action, attach to the skin during the cleaning process to exert its function and avoid being washed away. By adding a wrapped long - chain cationic raw material, the active ingredients are tightly adsorbed on the skin surface, acting on carbonylated proteins and oxidized lipids and not being washed away during the flushing process. The skin conditioner B can be, for example, any value between 0.05%, 0.1%, 0.15%, 0.2% or 0.05% - 0.2%.

[0042] The brightening complexion composition provided by this application includes skin conditioner A, polyglyceryl - 10 - laurate, taurine, and skin conditioner B. Skin conditioner A includes camellia extract, which has antioxidant properties of scavenging reactive oxygen species and enhancing antioxidant enzymes; polyglyceryl - 10 - laurate can significantly inhibit the glycation reaction, thereby protecting proteins in the skin from damage; taurine attaches to damaged carbonylated proteins to label the proteins, enabling them to be recognized by the proteasome. The enzymatic components of the proteasome shear the proteins into small peptide fragments, allowing them to be further degraded by other enzymes in the cytoplasm of the cell; polyquaternium - 51 in skin conditioner B is a living body - affinity polymer with phosphorylcholine groups. It can encapsulate the above three components and, by means of electrostatic action, attach to the skin during the cleaning process to exert its function and avoid being washed away.

[0043] In some embodiments, by mass percentage, it includes:

[0044]

[0045] In some embodiments, by mass percentage, it includes:

[0046]

[0047] In some embodiments, by mass percentage, it includes:

[0048]

[0049] In some embodiments, the skin conditioner A is in parts by weight and includes the following components: 45 - 50 parts of 1,3 - propanediol, 45 - 54 parts of water, and 1 - 5 parts of camellia extract.

[0050] Among them, 1,3-propylene glycol can be, for example, 45 parts, 46 parts, 47 parts, 48 ​​parts, 49 parts, 50 parts or any value between 45-50 parts; water can be, for example, 45 parts, 46 parts, 47 parts, 48 ​​parts, 49 parts, 50 parts, 51 parts, 52 parts, 53 parts, 54 parts or any value between 45-54 parts; camellia extract can be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts or any value between 1-5 parts.

[0051] In some embodiments, the skin conditioner B comprises the following components by weight: 90-94 parts of water, 1-5 parts of polyquaternium-51, and 1-9 parts of phenoxyethanol.

[0052] Among them, water can be, for example, 90 parts, 91 parts, 92 parts, 93 parts, 94 parts or any value between 90-94 parts; polyquaternium-51 can be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts or any value between 1-5 parts; phenoxyethanol can be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts or any value between 1-9 parts.

[0053] The present application also provides the use of the above-mentioned skin lightening composition in the preparation of skin care products.

[0054] The present application also provides a makeup removing cream, comprising the above-mentioned skin lightening composition.

[0055] The makeup remover provided in this application uses the above-mentioned skin lightening composition, starting from the skin mechanism that causes dull skin tone, combined with the characteristics of the makeup remover category, and comprehensively brightens the skin tone in four dimensions, including reducing free radicals on the skin surface, inhibiting the synthesis of AGES, the end product of the glycation reaction, removing the source of dull skin - carbonyl protein and lipid peroxide, reducing the accumulation of waste keratin on the skin surface, solving the problem that the effect of single-sided reduction is not obvious, and through the synergistic effect of the four aspects, it is more effective to solve the dull skin tone and brighten the skin tone. Make use of the oil-dissolving property of the makeup remover to dissolve the waste keratin attached to the skin surface, so as to achieve the purpose of brightening the skin tone.

[0056] In some embodiments, the composition, measured by mass percentage, includes:

[0057]

[0058] The present application also provides a method for preparing the above-mentioned makeup remover cream, comprising:

[0059] Add isohexadecane, coconut oil alcohol monocaprylate / caprate, PEG-20 glyceryl triisostearate and synthetic wax into a reaction container, heat up and stir to obtain phase A;

[0060] After cooling down phase A, add the remaining ingredients in sequence and stir to obtain a cleansing cream.

[0061] In some embodiments, the temperature for heating up is 92 - 95 °C, and the temperature for cooling down is 80 - 85 °C.

[0062] The following will describe the implementation schemes of the present application in detail with specific examples. However, those skilled in the art will understand that the following examples are only used to illustrate the present application and should not be regarded as limiting the scope of the present application. For those not specified in the examples, the operations are carried out under conventional conditions or conditions recommended by the manufacturer. For the reagents or instruments not specified for the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0063] In the following experiments, the specific components of skin conditioner A in Examples 1 to 4 and Comparative Examples 1 to 6 are as follows by mass fraction: 50 parts of 1,3 - propanediol, 48 parts of water, and 2 parts of camellia extract; the specific components of skin conditioner B are as follows by mass fraction: 2 parts of polyquaternium - 51, 94 parts of water, and 1 part of phenoxyethanol. The specific components of skin conditioner A in Example 5 are as follows by mass fraction: 50 parts of 1,3 - propanediol, 45 parts of water, and 5 parts of camellia extract; the specific components of skin conditioner B are as follows by mass fraction: 3 parts of polyquaternium - 51, 94 parts of water, and 3 parts of phenoxyethanol.

[0064] Example 1

[0065] Example 1 provides a skin - brightening composition, by mass percentage, including: 0.1% of skin conditioner A; 0.1% of polyglyceryl - 10 - laurate; 0.1% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0066] Example 2

[0067] Example 2 provides a skin - brightening composition, by mass percentage, including: 0.5% of skin conditioner A; 0.5% of polyglyceryl - 10 - laurate; 0.5% of thiotaurine; 0.1% of skin conditioner B, and the balance is water.

[0068] Example 3

[0069] Example 3 provides a skin - brightening composition, by mass percentage, including: 0.1% of skin conditioner A; 0.05% of polyglyceryl - 10 - laurate; 0.05% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0070] Example 4

[0071] Example 4 provides a skin - brightening composition, by mass percentage, including: 0.05% of skin conditioner A; 0.1% of polyglyceryl - 10 - laurate; 0.1% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0072] Example 5

[0073] Example 5 provides a skin brightening composition, which comprises, by mass percentage: 0.1% of skin conditioner A; 0.1% of polyglyceryl-10 laurate; 0.1% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0074] Comparative Example 1

[0075] Comparative Example 1 provides a skin brightening composition, which comprises, by mass percentage: 0.1% of polyglyceryl-10 laurate; 0.1% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0076] Comparative Example 2

[0077] Comparative Example 2 provides a skin brightening composition, which comprises, by mass percentage: 0.1% of skin conditioner A; 0.05% of skin conditioner B, and the balance is water.

[0078] Comparative Example 3

[0079] Comparative Example 3 provides a skin brightening composition, which comprises, by mass percentage: 0.1% of skin conditioner A; 0.1% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0080] Comparative Example 4

[0081] Comparative Example 4 provides a skin brightening composition, which comprises, by mass percentage: 0.1% of skin conditioner A; 0.1% of polyglyceryl-10 laurate; 0.05% of skin conditioner B, and the balance is water.

[0082] Comparative Example 5

[0083] Comparative Example 5 provides a skin brightening composition, which comprises, by mass percentage: 0.1% of polyglyceryl-10 laurate; 0.1% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0084] Comparative Example 6

[0085] Comparative Example 6 provides a skin brightening composition, which comprises, by mass percentage: 0.1% of tea extract; 0.1% of polyglyceryl-10 laurate; 0.1% of thiotaurine; 0.05% of skin conditioner B, and the balance is water.

[0086] The formulations of the skin brightening compositions of each example and comparative example are shown in Table 1.

[0087] Table 1 Formulations of the skin brightening compositions of each example and comparative example

[0088]

[0089]

[0090] The skin - brightening compositions of each example and comparative example were respectively subjected to efficacy tests.

[0091] 1. Antioxidant experiment - DPPH scavenging rate

[0092] Prepare a 0.1 mM DPPH solution and a certain amount of the test solution. Use a 96 - well plate, with three groups, and set 3 replicates for each group. The addition amount per well and the distribution on the 96 - well plate are as follows, and add the samples:

[0093] sample (sample group): 100 μL of sample solution + 100 μL of DPPH alcohol solution (3 holes for each concentration);

[0094] blank (blank group): 100 μL of sample solution + 100 μL of absolute ethanol (3 holes for each concentration);

[0095] control (control group): 100 μL of DPPH alcohol solution + 100 μL of water;

[0096] Use an ultraviolet spectrophotometer to measure the absorbance at 517 nm, take the average value, and calculate the DPPH scavenging rate of the sample at each concentration through the following formula: scavenging rate = (1 - (A(sample)-A(blank)) / A(control))×100%.

[0097] The test results are shown in Table 2.

[0098] Table 2 DPPH scavenging rates of the skin - brightening compositions of each example and comparative example

[0099]

[0100] According to Table 2, it can be seen that the antioxidant effect of Comparative Example 1 without camellia extract is the worst. The antioxidant effect of Comparative Example 6 with tea extract is much worse than that of Example 1 with camellia extract, proving that the use of camellia extract in this application has a better effect. Examples 1, Comparative Example 2, and Comparative Example 3 show that in terms of antioxidant effect, the synergistic effect between camellia extract and taurine is relatively obvious, and there is also a synergistic effect among camellia extract, taurine, and polyglyceryl - 10 - laurate. Example 2 shows that the higher the content of camellia extract, the better the antioxidant effect. Example 3 shows that too low contents of taurine and polyglyceryl - 10 - laurate will also affect the antioxidant effect.

[0101] 2. Anti - glycation experiment

[0102] Preparation of glucose (Glu) solution: 18 g of a certain mass of Glu was dissolved in PBS solution to make its concentration 1 M, and then filtered and sterilized.

[0103] 0.1 M carbonate buffer solution with pH 10.8: 9.54 g of anhydrous Na 2 CO 3 and 0.84 g of NaHCO 3 were both of analytical purity, and distilled water was added to make up the volume to 1000 ml.

[0104] NBT staining test solution: 100 mg was dissolved in the above carbonate buffer solution.

[0105] Prepare detection substance solutions with certain concentrations, and the concentration gradients were referred to as 1, 0.5, 10(-1), 10(-2) g.L(-1). They were respectively added to the glucose / bovine serum albumin glycation system. At the same time, other controls were set up: (1) a complete non-enzymatic glycation system without the detection substance; (2) a system without Glu without the detection substance; (3) a glycation system with the detection substance without albumin; (4) a glycation system with the detection substance without Glu. Incubate at a certain temperature for a period of time. After incubation, take 0.05 ml of the reaction solution, add 4 ml of NBT, incubate at 37 °C for 15 min, then terminate the reaction with 0.1 ml of 15% acetic acid and cold bath, and then colorimetric at 530 nm on a spectrophotometer.

[0106] Calculate the inhibitory rate (IR) according to the following formula:

[0107]

[0108] The test results are shown in Table 3.

[0109] Table 3 Anti-glycation data of the skin brightening compositions of each example and comparative example

[0110]

[0111] It can be seen from Table 3 that the anti-glycation effect of Comparative Example 3 without polyglyceryl-10 laurate is the worst, and the content of polyglyceryl-10 laurate added in Example 3 is relatively low, and the anti-glycation effect is also poor. It can be seen from Comparative Example 4 and Comparative Example 5 that in terms of the anti-glycation effect, the synergistic effect of polyglyceryl-10 laurate and taurine is less than the synergistic effect of polyglyceryl-10 laurate and camellia extract. It can be seen from Example 1 and the comparative example that in terms of the anti-glycation effect, at appropriate dosages, the synergistic effect of polyglyceryl-10 laurate, taurine and camellia extract is stronger than the synergistic effect of the two. Example 2 shows that the higher the content of polyglyceryl-10 laurate, the better the anti-glycation effect. In Example 4, the dosage of camellia extract is relatively small, but it instead improves the anti-glycation effect.

[0112] 3. Carbonyl Protein Clearance Experiment

[0113] Use an oxidant to induce protein carbonylation as a positive control.

[0114] Mix another part of the protein sample with the sample to be tested to simulate the process of clearing carbonylated proteins.

[0115] Extract the target protein with lysis buffer, centrifuge to remove cell debris, and collect the supernatant. Determine the protein concentration using the BCA or Bradford method and adjust it to 1 - 2 mg / mL. Prepare a 10 mM DNPH solution (dissolved in 2 M HCl). Add the following amounts to each well and distribute them in a 96 - well plate for the samples:

[0116] Experimental group: 100 μL protein sample + 100 μL DNPH solution.

[0117] Blank control group: 100 μL protein sample + 100 μL 2 M HCl (without DNPH).

[0118] Incubate with shaking at room temperature in the dark for 1 hour (or 30 minutes at 37 °C in the dark), and mix every 10 minutes. Add 500 μL of 20% trichloroacetic acid (TCA), and precipitate the protein in an ice bath for 10 minutes. Centrifuge for 10 minutes and discard the supernatant. Wash the precipitate 3 times with a mixture of ethanol - ethyl acetate (1:1, v / v), and then centrifuge to remove unbound DNPH and impurities. Dry at room temperature for 5 - 10 minutes to remove the residual solvent. Dissolve the precipitate with 6 M guanidine hydrochloride solution and vortex until completely dissolved. Finally, measure the absorbance of the experimental group and the blank control group at 370 nm.

[0119]

[0120] · Where, V 总 : Dissolution volume (mL); ε: Molar extinction coefficient of DNP - hydrazone (22,000 M -1 cm -1 );

[0121] · d: Light path of the cuvette (cm); C 蛋白 : Protein concentration (mg / mL).

[0122] The test results are shown in Table 4.

[0123] Table 4 Anti - carbonyl data of the skin - brightening compositions of each example and comparative example

[0124]

[0125] As can be seen from Table 4, Comparative Example 4 without added taurine has the worst anti-carbonyl effect. In Example 3, the content of added taurine is relatively low, and the anti-carbonyl effect is also poor. From Comparative Example 4 and Comparative Example 5, it can be seen that in terms of the anti-carbonyl effect, the synergistic effect of camellia extract and taurine is less than that of polyglyceryl-10 laurate and taurine. From Example 1 and the comparative examples, it can be seen that in terms of the anti-carbonyl effect, when taurine, polyglyceryl-10 laurate and camellia extract are used in appropriate amounts, the synergistic effect of the three is stronger than that of the two. Example 2 shows that the higher the content of taurine, the better the anti-carbonyl effect.

[0126] Application Example

[0127] To sum up, Example 1 in this application is the most preferred brightening complexion composition scheme, which can achieve an ideal effect with a relatively low dosage. Add the brightening complexion composition of Example 1 with synergistic effect to the cleansing balm system to prepare a cleansing product, and compare and verify the irritation of the finished product with the blank sample and the best-selling products on the market respectively.

[0128] This application provides a cleansing balm, which includes, by mass percentage: isocetane 28.65%; coco-caprylate / caprate 55%; PEG-20 glyceryl triisostearate 10%; synthetic wax 6%; camellia extract 0.1%; polyglyceryl-10 laurate 0.1%; taurine 0.1%; polyquaternium-51 0.05%.

[0129] This application provides a preparation method of a cleansing balm, including:

[0130] Put isocetane, coco-caprylate / caprate, PEG-20 glyceryl triisostearate and synthetic wax into the main pot, turn on the heating and stirring, heat up to 92-95 °C, and stir until all are dissolved and transparent to obtain Phase A.

[0131] Cool down to 80-85 °C, and sequentially add camellia extract, polyglyceryl-10 laurate, taurine, and polyquaternium-51, stir to dissolve completely, and homogenize and stir until completely uniform.

[0132] After stirring evenly, take a sample to obtain the cleansing balm.

[0133] The formula of the blank sample, by mass percentage, includes: isocetane 29%; coco-caprylate / caprate 55%; PEG-20 glyceryl triisostearate 10%; synthetic wax 6%.

[0134] Efficacy Test - Complexion Brightening Experiment

[0135] Volunteers uniformly marked 3 skin areas with an area of 3 cm × 3 cm on each of the left and right cheeks as the test areas, with at least a 2-cm interval between each area. The 3 test areas on the left cheek were sequentially numbered as L1, L2, and L3, and the 3 test areas on the right cheek were sequentially numbered as R1, R2, and R3. Sit still for 30 min, use a microscope to collect images of each test area, and use a spectrophotometer to measure the L*, a*, and b* values of the skin before cleaning.

[0136] According to the dosage of 2.00 mg / cm 2 , evenly apply the blank cleansing balm sample and the cleansing balm sample of this case on each test area on the flexor side of the left forearm; knead the test area 20 times, rinse it with clean water, and gently blot the water with a tissue. Sit still for 30 min, use a microscope to collect images of each test area, and use a spectrophotometer to measure the L*, a*, and b* values of the skin after cleansing and makeup removal.

[0137] L* represents black and white degree. The larger the L* value, the more the skin tends to be white, and vice versa, it tends to be black; a* represents red and green degree. The larger the a* value, the more the skin tends to be red, and vice versa, it tends to be green; b* represents blue and yellow degree. The larger the b* value, the more the skin tends to be yellow. The laboratory maintains constant temperature and humidity, the test temperature is 23°C to 24°C, and the relative humidity is 50% to 60%, and vice versa, it tends to be blue.

[0138] Compared with the initial state, the skin color difference after cleansing and makeup removal is denoted as △E (after cleaning). △E can comprehensively reflect the three-dimensional change of skin chromaticity. The larger the △E value, the more obvious the skin color change, and the smaller the △E value, the closer the skin color. Calculate the brightening ability through △E.

[0139] The test results are shown in Table 5.

[0140] Table 5 Color difference of the cleansing balm of this application after cleaning the skin

[0141]

[0142] According to Table 5, it can be seen that the color difference of the cleansing balm of this application after cleaning the skin is the largest, and the color difference is significantly improved compared with the existing cleansing balms on the market, indicating that the cleansing balm of this application can brighten the skin color and improve the problem of dull skin color after use.

[0143] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of this application, rather than to limit it; although this application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of this application.

[0144] In addition, those skilled in the art will understand that although some embodiments herein include certain features included in other embodiments rather than other features, the combination of features of different embodiments means that it is within the scope of the present application and forms different embodiments. For example, in the claims above, any one of the claimed embodiments can be used in any combination. The information disclosed in this background section is only intended to deepen the understanding of the overall background art of the present application, and should not be regarded as an admission or any form of implication that this information constitutes the prior art known to those skilled in the art.

Claims

1. A skin lightening composition, characterized in that: In terms of mass percentage, it includes:

2. The skin lightening composition according to claim 1, characterized in that: In terms of mass percentage, it includes:

3. The skin lightening composition according to claim 2, characterized in that: In terms of mass percentage, it includes:

4. The skin lightening composition according to claim 3, characterized in that: In terms of mass percentage, it includes:

5. The skin lightening composition according to any one of claims 1 to 4, characterized in that: The skin conditioner A comprises the following components by weight: 45-50 parts of 1,3-propylene glycol, 45-54 parts of water, and 1-5 parts of camellia extract; the skin conditioner B comprises the following components by weight: 90-94 parts of water, 1-5 parts of polyquaternium-51, and 1-9 parts of phenoxyethanol.

6. Use of the skin lightening composition according to any one of claims 1 to 5 in the preparation of skin care products.

7. A makeup removing cream, characterized in that: The composition for brightening skin color comprises the composition according to any one of claims 1 to 5.

8. The makeup removing cream according to claim 7, characterized in that: Calculated by mass percentage, it also includes: 45%-60% of solvent, 5%-25% of emulsifier, and 3%-8% of oil phase thickener.

9. The makeup removing cream according to claim 8, characterized in that: In terms of mass percentage, it includes:

10. A method for preparing the makeup remover cream according to claim 9, characterized in that: include: Add isohexadecane, coconut oil alcohol monocaprylate / caprate, PEG-20 glyceryl triisostearate and synthetic wax into a reaction container, heat up and stir to obtain phase A; After cooling down phase A, add the remaining ingredients in sequence and stir to obtain a cleansing cream; Optionally, the heating temperature is 92-95°C, and the cooling temperature is 80-85°C.

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