Enzymolysis traditional Chinese medicine decoction dry powder preparation and preparation method thereof

Abstract

The invention discloses an enzymolysis traditional Chinese medicine decoction dry powder preparation and a preparation method thereof, and belongs to the field of biomedicine.The enzymolysis traditional Chinese medicine decoction dry powder preparation comprises traditional Chinese medicine components, enzyme and a carrier, and the enzyme is matched with the traditional Chinese medicine components; the carrier comprises an adsorption body and a blocking layer wrapping the adsorption body; wherein the adsorption body is a modified porous yeast cell wall; the modified porous yeast cell wall is a modified substance obtained by performing enzymolysis and chambering on the yeast cell wall through beta-1, 3-glucanase and then performing carboxymethyl chitosan surface modification. According to the enzymolysis traditional Chinese medicine decoction dry powder preparation, the traditional Chinese medicine zymolyte is enriched through the adsorption body, then the adsorption body is wrapped by the blocking layer to prevent leakage of the traditional Chinese medicine zymolyte, and the dry powder preparation which can be stored for a long time is obtained.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine and relates to an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation and a preparation method thereof. Background Art

[0002] Traditional Chinese medicine components are the main components of traditional Chinese medicine health-preserving decoctions. At present, the traditional Chinese medicine component preparations for traditional Chinese medicine health-preserving decoctions mainly adopt two forms: one is a prefabricated liquid decoction, which is convenient for processing and has a good taste, but has a short shelf life (usually requires refrigeration and does not exceed 7 days), and has a high transportation cost; the other is to directly use traditional Chinese medicinal materials. The medicinal materials have a large volume, a long boiling time (usually 2 - 3 hours, which is not conducive to obtaining effective components), and the medicinal residues affect the taste (such as bitter taste or rough texture). There is a lack of a traditional Chinese medicine component preparation that is both convenient for storage and convenient for obtaining effective components of traditional Chinese medicine. Summary of the Invention

[0003] The purpose of the present invention is to provide an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation and a preparation method thereof, which solve the problem that there is currently a lack of a traditional Chinese medicine component preparation for traditional Chinese medicine health-preserving decoctions that is both convenient for storage and convenient for obtaining effective components of traditional Chinese medicine.

[0004] The technical solution adopted by the present invention is as follows: An enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation, comprising traditional Chinese medicine components, an enzyme and a carrier, wherein the enzyme is adapted to the traditional Chinese medicine components; The carrier includes an adsorbent and a blocking layer that wraps the adsorbent; wherein, the adsorbent is a modified porous yeast cell wall; The modified porous yeast cell wall is a modified product obtained by enzymatically expanding the pores of the yeast cell wall with β-1,3-glucanase and then performing surface modification with carboxymethyl chitosan.

[0005] In the present invention, enzymes are used to enzymatically hydrolyze traditional Chinese medicine components to obtain enzymatically hydrolyzed traditional Chinese medicine products. The present invention utilizes the directional enzyme β-1,3-glucanase to expand the pores of yeast cell walls, increase their porosity, and form a porous structure. The β-1,3-glucanase only hydrolyzes the β-1,3 bonds of the yeast wall to form pores with a size of 2-5 nm, which allows small molecule substances to enter and blocks hydrolytic enzymes for macromolecular substances such as traditional Chinese medicine components, avoiding the occupancy competition of macromolecular substances such as enzymes and small molecule traditional Chinese medicine enzymatically hydrolyzed products on the yeast cell wall, and providing sufficient storage space for traditional Chinese medicine enzymatically hydrolyzed products. The pore expansion in this application does not refer to expanding the pore diameter, but rather expanding the proportion of the number of pores. After the directional enzyme pore expansion, the present application further modifies the pore-expanded yeast cell wall with carboxymethyl chitosan. Without affecting the release of the enzymatically hydrolyzed product after the dry powder is redissolved, carboxymethyl chitosan enhances the adsorption strength of the yeast cell wall for substances entering its internal pores. The present invention utilizes pore size screening and adsorption enhancement to achieve targeted adsorption of small molecule enzymatically hydrolyzed products, which is beneficial to increasing the content of small molecule enzymatically hydrolyzed products in the final dry powder preparation and reducing the adsorption effect of main macromolecular substances such as enzymes on small molecule enzymatically hydrolyzed products.

[0006] After the modified porous yeast cell wall adsorbs the traditional Chinese medicine enzymatically hydrolyzed product in the present invention, in order to prevent the traditional Chinese medicine enzymatically hydrolyzed product from leaking from the modified porous yeast cell wall, a sealing layer is used to wrap the modified porous yeast cell wall adsorbed with the traditional Chinese medicine enzymatically hydrolyzed product, which is also beneficial for the long-term preservation of the traditional Chinese medicine enzymatically hydrolyzed product.

[0007] Further, the modified porous yeast cell wall is prepared by the following method: A1. At 50 °C, use 10 U / g of β-1,3-glucanase to enzymatically hydrolyze the yeast cell wall for 1.5 - 2 hours to obtain a porous yeast cell wall with 2 - 5 nm micropores distributed thereon; A2. Dissolve carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution, add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, and stir and react to obtain an activated carboxymethyl chitosan solution; A3. Disperse the porous yeast cell wall in MES buffer to obtain a porous yeast cell wall suspension; A4. Add the activated carboxymethyl chitosan solution to the porous yeast cell wall suspension, stir and react for 4 - 5 hours, then add glycine, stir for 30 - 40 minutes and then separate to obtain a precipitate, which is then washed and freeze-dried to obtain the modified porous yeast cell wall.

[0008] Further, the mass ratio of the porous yeast cell wall to carboxymethyl chitosan is 9 - 10:1.

[0009] Furthermore, the addition amount of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is 2% of the mass of carboxymethyl chitosan, and the addition amount of N-hydroxysuccinimide is 1% of the mass of carboxymethyl chitosan.

[0010] Furthermore, the blocking layer comprises the following components: sodium alginate, gelatin, resistant dextrin and a crosslinking agent.

[0011] Furthermore, the traditional Chinese medicine components comprise the following components: ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus, and the mass ratio of ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus is 4:2:2:1.

[0012] In combination with the actual R & D situation, the present invention provides a specific traditional Chinese medicine component.

[0013] Furthermore, the enzyme is a composite enzyme, the mass ratio of the composite enzyme to the traditional Chinese medicine components is 1:50 - 52, the composite enzyme comprises the following components: cellulase, protease, amylase, β-glucosidase, the pH of enzymatic hydrolysis is 5 - 6, and the mass ratio of cellulase, protease, amylase, β-glucosidase is 1:0.5:0.5:1.

[0014] The enzyme in the present invention is selected based on the traditional Chinese medicine components.

[0015] Furthermore, the mass ratio of resistant dextrin, sodium alginate to gelatin is (3 - 4):(1 - 2):1.

[0016] A preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation comprises the following steps: S1. Obtain a traditional Chinese medicine composition, including ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus, boil the traditional Chinese medicine composition in water for 1 - 1.5 h, and filter to obtain a primary traditional Chinese medicine extract; S2. Use beer yeast cell wall as a raw material, perform enzymatic treatment with β-1,3-glucanase for pore expansion, then perform surface modification with carboxymethyl chitosan, filter and dry to obtain a modified porous yeast cell wall; S3. Cool the primary traditional Chinese medicine extract to 50 °C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, β-glucosidase, perform enzymatic hydrolysis at 50 °C for 2 hours, add the modified porous yeast cell wall, continue to react for 1 hour, and filter to obtain a modified porous yeast cell wall loaded with enzymatically hydrolyzed traditional Chinese medicine components; S4. Add resistant dextrin to water, stir until completely dissolved, then add sodium alginate and gelatin, stir at 50 °C for 1 h to obtain a liquid mixture of the blocking layer, then disperse the modified porous yeast cell wall loaded with enzymatically hydrolyzed traditional Chinese medicine components in the liquid mixture of the blocking layer, stir evenly, and then dropwise add CaCl 2The crosslinking agent undergoes a crosslinking reaction. After the reaction ends, filtration and spray drying are carried out to obtain dry powder of modified porous yeast cell walls loaded with enzymatically hydrolyzed traditional Chinese medicine components wrapped by a blocking layer, which is the dry powder preparation of enzymatically hydrolyzed traditional Chinese medicine decoction.

[0017] Furthermore, the modified porous yeast cell walls and the initial extract of traditional Chinese medicine are mixed in a ratio of 15 - 20% w / v.

[0018] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are as follows: 1. The dry powder preparation of enzymatically hydrolyzed traditional Chinese medicine decoction of the present invention enriches the enzymatically hydrolyzed traditional Chinese medicine substances with an adsorbent, and then wraps the adsorbent with a blocking layer to prevent the leakage of the enzymatically hydrolyzed traditional Chinese medicine substances, obtaining a dry powder preparation that can be stored for a long time. 2. In the present invention, the adsorbent is modified porous yeast cell walls. The porous yeast cell walls modified with carboxymethyl chitosan have a targeted and efficient adsorption effect on the enzymatically hydrolyzed traditional Chinese medicine substances. Moreover, after surface modification with carboxymethyl chitosan, it can not only significantly improve the thermal stability of the porous yeast cell walls but also block the influence of the enzymes for enzymatically hydrolyzing traditional Chinese medicine on the yeast cell walls, enabling the modified porous yeast cell walls to stably exist in the weakly acidic environment of enzymatically hydrolyzing traditional Chinese medicine without hydrolysis. However, when the dry powder preparation of enzymatically hydrolyzed traditional Chinese medicine decoction of the present application is added to the health preservation decoction and subjected to high-pressure boiling, at a temperature above 100 °C, along with the disintegration of the blocking layer, the modified porous yeast cell walls will rupture, releasing the adsorbed enzymatically hydrolyzed traditional Chinese medicine substances. The enzymatically hydrolyzed traditional Chinese medicine substances after enzymatic hydrolysis enter the traditional Chinese medicine health preservation decoction to play the corresponding health preservation role, and the ruptured modified porous yeast cell walls are small in volume and do not need to be removed, which is also beneficial to health. 3. In the present invention, the blocking layer mainly consists of sodium alginate, gelatin, and resistant dextrin, which can rapidly disintegrate at a temperature above 100 °C and will not have an obvious impact on increasing the viscosity of the decoction after disintegration. 4. The preparation method of the dry powder preparation of enzymatically hydrolyzed traditional Chinese medicine decoction of the present invention obtains a dry powder preparation that can stably adsorb and enrich the enzymatically hydrolyzed traditional Chinese medicine substances in an enzymatic hydrolysis environment at about 50 °C, and can rapidly disintegrate and release the internal enzymatically hydrolyzed traditional Chinese medicine substances in a high-pressure health preservation decoction boiling environment above 100 °C. After being redissolved and dispersed in the health preservation decoction, the dry powder preparation of the present invention will not bring solid substances with a large volume ratio and has little impact on the taste of the health preservation decoction. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required to be used in the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, and therefore should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings, where: Figure 1This is the surface SEM image of the modified porous yeast cell wall powder particles of the present invention. Detailed implementation manners

[0020] In order to make the objectives, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention, that is, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Usually, the components of the embodiments of the present invention described and shown in the accompanying drawings herein can be arranged and designed in various different configurations.

[0021] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative efforts fall within the scope of protection of the present invention.

[0022] It should be noted that relational terms such as "first" and "second" are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising one..." does not exclude the existence of additional identical elements in the process, method, article or device including the said element.

[0023] The features and performance of the present invention will be further described in detail below with reference to the embodiments.

[0024] Embodiment 1: An enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation provided by a preferred embodiment of the present invention includes traditional Chinese medicine components, enzymes and carriers, wherein the enzymes are adapted to the traditional Chinese medicine components; The carrier includes an adsorbent and a blocking layer that wraps the adsorbent; wherein, the adsorbent is a modified porous yeast cell wall; The modified porous yeast cell wall is a modified product obtained by surface modification of carboxymethyl chitosan after the yeast cell wall is enzymatically hydrolyzed and enlarged with β-1,3-glucanase.

[0025] The modified porous yeast cell wall is prepared by the following method: A1. At 50 °C, use 10 U / g of β-1,3-glucanase to enzymatically hydrolyze the yeast cell wall for 1.5 - 2 hours to obtain a porous yeast cell wall with micropores of 2 - 5 nm distributed thereon; A2. Dissolve carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution. Add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, and stir to react to obtain an activated carboxymethyl chitosan solution; A3. Disperse the porous yeast cell wall in MES buffer to obtain a porous yeast cell wall suspension; A4. Add the activated carboxymethyl chitosan solution to the porous yeast cell wall suspension, stir and react for 4 - 5 hours, then add glycine, stir for 30 - 40 minutes and separate to obtain a precipitate, which is then washed and freeze-dried to obtain a modified porous yeast cell wall.

[0026] The specific surface area of the porous yeast cell wall after pore expansion in the present invention is significantly increased compared to that before pore expansion. As Figure 1 shown in the surface SEM image of the modified porous yeast cell wall powder particles, there are a large number of pore structures distributed.

[0027] The mass ratio of the porous yeast cell wall to carboxymethyl chitosan is 9 - 10:1.

[0028] The addition amount of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is 2% of the mass of carboxymethyl chitosan, and the addition amount of N-hydroxysuccinimide is 1% of the mass of carboxymethyl chitosan.

[0029] The blocking layer comprises the following components: sodium alginate, gelatin, resistant dextrin and a cross-linking agent.

[0030] The traditional Chinese medicine components comprise the following components: ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus. The mass ratio of ginseng, polygonatum sibiricum, cordyceps militaris, astragalus membranaceus is 4:2:2:1. The traditional Chinese medicine components in this example are one of the actually developed traditional Chinese medicine components, and the present invention is not limited to the above traditional Chinese medicine components only, The enzyme is a composite enzyme. The mass ratio of the composite enzyme to the traditional Chinese medicine components is 1:50 - 52. The composite enzyme comprises the following components: cellulase, protease, amylase, β-glucosidase. The pH of enzymatic hydrolysis is 5 - 6, and the mass ratio of cellulase, protease, amylase, β-glucosidase is 1:0.5:0.5:1.

[0031] The mass ratio of resistant dextrin, sodium alginate to gelatin is (3 - 4):(1 - 2):1.

[0032] A preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation comprises the following steps: S1. Obtain a traditional Chinese medicine composition, including ginseng, polygonatum sibiricum, cordyceps militaris, and astragalus membranaceus. Boil the traditional Chinese medicine composition in water for 1 - 1.5 h, and filter to obtain a primary extract of traditional Chinese medicine; S2. Use beer yeast cell wall as raw material, perform enzyme treatment with β-1,3-glucanase to expand pores, and then use carboxymethyl chitosan for surface modification. After filtration and drying, obtain modified porous yeast cell wall; S3. Cool the primary extract of traditional Chinese medicine to 50 °C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, and β-glucosidase. After enzymatic hydrolysis at 50 °C for 2 hours, add the modified porous yeast cell wall and continue to react for 1 hour. Filter to obtain modified porous yeast cell wall loaded with enzymatically hydrolyzed components of traditional Chinese medicine; S4. Add resistant dextrin to water and stir until completely dissolved, then add sodium alginate and gelatin. Stir at a constant temperature of 50 °C for 1 h to obtain a liquid mixture of the blocking layer. Then disperse the modified porous yeast cell wall loaded with enzymatically hydrolyzed components of traditional Chinese medicine in the liquid mixture of the blocking layer, stir evenly, and then dropwise add CaCl 2 Crosslinking agent for crosslinking reaction. After the reaction, filter and spray dry to obtain dry powder of modified porous yeast cell wall loaded with enzymatically hydrolyzed components of traditional Chinese medicine wrapped by the blocking layer, which is the dry powder preparation of enzymatically hydrolyzed traditional Chinese medicine decoction.

[0033] The modified porous yeast cell wall and the primary extract of traditional Chinese medicine are mixed in a ratio of 15% w / v.

[0034] Example 2: Based on Example 1, the difference from Example 1 in this example is that: the modified porous yeast cell wall and the primary extract of traditional Chinese medicine are mixed in a ratio of 17.5% w / v.

[0035] Example 3: Based on Example 1, the difference from Example 1 in this example is that: the modified porous yeast cell wall and the primary extract of traditional Chinese medicine are mixed in a ratio of 20% w / v.

[0036] Example 4: Based on Example 1, the present invention gives another solution of a traditional Chinese medicine composition with different functions and its corresponding enzymes. The difference from Example 1 is that: the traditional Chinese medicine composition is agaricus blazei, angelica sinensis, and polygonatum odoratum, the enzymes are cellulase, protease, and amylase, the enzymatic hydrolysis temperature is 50 °C, and the enzymatic hydrolysis pH is 5; the mass ratio of agaricus blazei, angelica sinensis, and polygonatum odoratum is 5:3:2, and the mass ratio of cellulase, protease, and amylase is 1:0.5:1. This example is basically equivalent to Example 1 in terms of adsorption effect, disintegration time, and change in solution viscosity after disintegration.

[0037] Comparative Example 1: This comparative example is based on Example 2, and the difference from Example 2 is that the mass ratio of porous yeast cell wall to carboxymethyl chitosan is 8:1.

[0038] Comparative Example 2: This comparative example is based on Example 2, and the difference from Example 2 is that the mass ratio of porous yeast cell wall to carboxymethyl chitosan is 11:1.

[0039] Comparative Example 3: This comparative example is based on Example 2, and the difference from Example 2 is that the carrier does not include a blocking layer. A preparation method of an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation includes the following steps: S1. Obtain a traditional Chinese medicine composition including ginseng, polygonatum sibiricum, cordyceps militaris, and astragalus membranaceus. Boil the traditional Chinese medicine composition in water for 1 - 1.5 h, and filter to obtain a primary traditional Chinese medicine extract; S2. Use beer yeast wall as a raw material, perform enzyme treatment with β-1,3-glucanase for pore expansion, and then use carboxymethyl chitosan for surface modification. After filtration and drying, obtain a modified porous yeast cell wall; S3. Cool the primary traditional Chinese medicine extract to 50 °C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, and β-glucosidase. After enzymatic hydrolysis at 50 °C for 2 hours, add the modified porous yeast cell wall, continue to react for 1 hour, filter to obtain a modified porous yeast cell wall loaded with enzymatically hydrolyzed traditional Chinese medicine components, and spray dry to obtain an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation.

[0040] Comparative Example 4: This comparative example is based on Example 2, and the difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by directly performing carboxymethyl chitosan surface modification on yeast cell wall without enzymatic hydrolysis and pore expansion with β-1,3-glucanase. The modified porous yeast cell wall is prepared by the following method: A1. Dissolve carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution. Add 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, and stir and react to obtain an activated carboxymethyl chitosan solution; A2. Disperse yeast cell wall in MES buffer to obtain a yeast cell wall suspension; A3. Add the activated carboxymethyl chitosan solution to the yeast cell wall suspension, stir and react for 4 - 5 hours, then add glycine, stir for 30 - 40 minutes, separate to obtain a precipitate, and then wash and freeze-dry to obtain a modified porous yeast cell wall.

[0041] Comparative Example 5: This comparative example is based on Example 2. The difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by only enzymatically expanding the pores of the yeast cell wall with β-1,3-glucanase without surface modification with carboxymethyl chitosan.

[0042] The modified porous yeast cell wall is prepared by the following method: at 50 °C, the yeast cell wall is enzymatically hydrolyzed with 10 U / g β-1,3-glucanase for 1.5 - 2 hours to obtain a porous yeast cell wall with 2 - 5 nm micropores distributed thereon, followed by washing and freeze-drying to obtain the modified porous yeast cell wall.

[0043] Comparative Example 6: This comparative example is based on Example 2. The difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by enzymatically expanding the pores of the yeast cell wall with β-1,6-glucanase followed by surface modification with carboxymethyl chitosan. The method for enzymatically expanding the pores refers to the existing usage conditions of β-1,6-glucanase, and the enzymatic hydrolysis temperature is 55 °C.

[0044] Comparative Example 7: This comparative example is based on Example 2. The difference from Example 2 is that the modified porous yeast cell wall is a modified product obtained by enzymatically expanding the pores of the yeast cell wall with cellulase followed by surface modification with carboxymethyl chitosan. The method for enzymatically expanding the pores refers to the existing usage conditions of cellulase. The pore size is too large to prevent the adsorption of macromolecules and target the adsorption of small molecules.

[0045] Comparative Example 8: This comparative example is based on Example 2. The difference from Example 2 is that the adsorbent is mesoporous silica. A preparation method for an enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation includes the following steps: S1. Obtain a traditional Chinese medicine composition including ginseng, polygonatum, cordyceps militaris, and astragalus membranaceus. Boil the traditional Chinese medicine composition in water for 1 - 1.5 h, and filter to obtain a primary traditional Chinese medicine extract; S2. Cool the primary traditional Chinese medicine extract to 50 °C, adjust the pH to 5 - 6, then add cellulase, protease, amylase, and β-glucosidase, enzymatically hydrolyze at 50 °C for 2 hours, add mesoporous silica, and continue the reaction for 1 hour, and filter to obtain mesoporous silica loaded with enzymatically hydrolyzed traditional Chinese medicine components; S4. Add resistant dextrin to water and stir until completely dissolved, then add sodium alginate and gelatin, stir at 50 °C for 1 h to obtain a blocked layer liquid mixture. Then disperse the mesoporous silica loaded with enzymatically hydrolyzed traditional Chinese medicine components in the blocked layer liquid mixture, stir evenly, and then dropwise add CaCl 2 A cross-linking agent for cross-linking reaction. After the reaction is completed, filter and spray-dry to obtain a dry powder of mesoporous silica loaded with enzymatically hydrolyzed traditional Chinese medicine components wrapped by a blocked layer, which is the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation.

[0046] Comparative Example 9: Based on Example 2, the difference in this comparative example from Example 2 is that the blocking layer does not include resistant dextrin.

[0047] Test Example 1: Detect the adsorption rates of the adsorbents in Examples 1 - 3 and Comparative Examples 1 - 9 for ginsenosides and cordycepin in a test environment with a pH of 5 - 6 and a temperature of 50°C, and detect the disintegration time of the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparations prepared in Examples 1 - 3 and Comparative Examples 1 - 9 in an aqueous solution under a pressure of 120°C and the solution viscosity after disintegration; the results are shown in Table 1. The detection methods for the adsorption rate, disintegration time, and solution viscosity are all existing technologies and will not be elaborated here; Table 1 Performance detection of the adsorbent and the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation: .

[0048] Test Example 2: Detect the storage time of the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparations prepared in Examples 1 - 4 and Comparative Examples 1 - 9 and the toxicity detection after disintegration, and the detection results are shown in Table 2.

[0049] The detection method for the storage time is as follows: Seal the dry powder in an aluminum foil bag (humidity < 30%), take samples for detection every month, and detect the retention rate of active ingredients (HPLC method): such as changes in the peak areas of ginsenosides, cordycepin, etc., and the microbial limit (GB4789.2): It needs to meet the food standard (total number of colonies < 1,000 CFU / g); Endpoint determination: Terminate when the retention rate of active ingredients < 90% or the microorganisms exceed the standard; Acute toxicity (rat experiment): Refer to GB 15193.3 - 2014 "National Food Safety Standard Acute Oral Toxicity Test"; Table 2 Detection results of the storage stability and toxicity of the enzymatically hydrolyzed traditional Chinese medicine decoction dry powder preparation: .

[0050] Combining the data in Table 1 and Table 2, it can be seen that Example 2 of the present invention is the best solution.

[0051] The above are only the preferred embodiments of the present invention and are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements, and improvements made by any person skilled in the art within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. An enzymatic Chinese medicine decoction dry powder preparation, characterized in that: It includes a Chinese medicine component, an enzyme and a carrier, wherein the enzyme is compatible with the Chinese medicine component; The carrier includes an adsorbent and a blocking layer wrapping the adsorbent; wherein the adsorbent is a modified porous yeast cell wall; The modified porous yeast cell wall is a modified product obtained by enzymatic hydrolysis and pore expansion of the yeast cell wall by β-1,3-glucanase and then surface modification with carboxymethyl chitosan.

2. The enzymatic hydrolysis Chinese medicine decoction dry powder preparation according to claim 1, characterized in that: The modified porous yeast cell wall is prepared by the following method: A1. At 50°C, the yeast cell wall was enzymatically hydrolyzed using 10U / g β-1,3-glucanase for 1.5-2 hours to obtain a porous yeast cell wall with 2-5 nm micropores; A2, dissolving carboxymethyl chitosan in MES buffer to obtain a carboxymethyl chitosan solution, adding 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide to the carboxymethyl chitosan solution, stirring and reacting to obtain an activated carboxymethyl chitosan solution; A3, dispersing the porous yeast cell wall in MES buffer to obtain a porous yeast cell wall suspension; A4. Add the activated carboxymethyl chitosan solution to the porous yeast cell wall suspension, stir and react for 4-5 hours, then add glycine, stir for 30-40 minutes, separate to obtain a precipitate, wash and freeze-dry to obtain a modified porous yeast cell wall.

3. The enzymatic hydrolysis Chinese medicine decoction dry powder preparation according to claim 2, characterized in that: The mass ratio of porous yeast cell wall to carboxymethyl chitosan is 9-10:

1.

4. The enzymatic hydrolysis Chinese medicine decoction dry powder preparation according to claim 3, characterized in that: The added amount of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride is 2% of the mass of carboxymethyl chitosan, and the added amount of N-hydroxysuccinimide is 1% of the mass of carboxymethyl chitosan.

5. The enzymatic Chinese medicine decoction dry powder preparation according to claim 1, characterized in that: The blocking layer comprises the following components: sodium alginate, gelatin, resistant dextrin and a cross-linking agent.

6. The enzymatic hydrolysis Chinese medicine decoction dry powder preparation according to claim 1, characterized in that: The traditional Chinese medicine components include the following components: ginseng, polygonatum, cordyceps militaris, and astragalus. The mass ratio of ginseng, polygonatum, cordyceps militaris, and astragalus is 4:2:2:

1.

7. The enzymatic Chinese medicine decoction dry powder preparation according to claim 6, characterized in that: The enzyme is a composite enzyme, and the mass ratio of the composite enzyme to the traditional Chinese medicine component is 1:50-52. The composite enzyme includes the following components: cellulase, protease, amylase, and beta-glucosidase. The enzymolysis pH is 5-6, and the mass ratio of the cellulase, protease, amylase, and beta-glucosidase is 1:0.5:0.5:

1.

8. The enzymatic hydrolysis Chinese medicine decoction dry powder preparation according to claim 5, characterized in that: The mass ratio of resistant dextrin, sodium alginate and gelatin is (3-4): (1-2):

1.

9. A method for preparing an enzymatic Chinese medicine decoction dry powder preparation according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. Obtain a Chinese medicine composition, including ginseng, polygonatum, cordyceps militaris, and astragalus, add water to the Chinese medicine composition and boil for 1-1.5 hours, and filter to obtain a primary extract of the Chinese medicine; S2, using beer yeast wall as raw material, enzymatically treating with β-1,3-glucanase to expand the pores, then using carboxymethyl chitosan for surface modification, filtering and drying to obtain modified porous yeast cell wall; S3, cooling the Chinese medicine primary extract to 50°C, adjusting the pH to 5-6, adding cellulase, protease, amylase, and β-glucosidase, and adding modified porous yeast cell walls after enzymolysis at 50°C for 2 hours, continuing the reaction for 1 hour, and filtering to obtain modified porous yeast cell walls loaded with Chinese medicine enzymolysis components; S4. Add resistant dextrin into water and stir until completely dissolved, then add sodium alginate and gelatin, and stir at a constant temperature of 50°C for 1 h to obtain a blocking layer liquid mixture. Disperse the modified porous yeast cell wall loaded with Chinese medicine enzymatic hydrolysis components in the blocking layer liquid mixture, stir evenly, and then add CaCl2 cross-linking agent dropwise for cross-linking reaction. After the reaction is completed, filter and spray dry to obtain the modified porous yeast cell wall dry powder loaded with Chinese medicine enzymatic hydrolysis components wrapped in the blocking layer, which is the enzymatic Chinese medicine decoction dry powder preparation.

10. The method for preparing an enzymatic Chinese medicine decoction dry powder preparation according to claim 9, characterized in that: The modified porous yeast cell wall and the primary extract of traditional Chinese medicine are mixed in a ratio of 15-20% w / v.

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