Preparation for removing tear stains of pets

Abstract

The invention discloses an innovative preparation for removing tear stains of pets, the preparation is accurately fused with vitamins A, B, E and beta, xanthophyll, anthocyanin, omega-3 fatty acid, zinc sulfate, boric acid, levofloxacin and 0.9% normal saline, and the components synergistically exert power according to a specific dosage ratio of 100ml. From the source, the eye drops can maintain cornea transparency, promote retina metabolism and reduce abnormal secretion of tears by adjusting physiological functions of eyes, and also can resist infection and eliminate tear stains in multiple dimensions by means of bactericidal and anti-inflammatory components. Strict tests show that the anti-tear agent has a remarkable anti-tear effect and can obviously fade tear stains of most pets. Meanwhile, the preparation highly pays attention to safety, the components are mild and non-irritant, and the preparation is adaptive to fragile eyes of pets; stability is good, and long-term storage at normal temperature is worry-free; operation is convenient and easy for pet owners to master. The method has the advantages of environmental protection concept, simple package and sustainable raw material weight, and provides a high-quality solution for the tear stain problem of pets.

Description

Technical Field

[0001] The present invention belongs to the technical field related to pet supplies, and particularly relates to a preparation for removing pet tear stains. Background Art

[0002] In modern society, pets have become an indispensable member of many families, and people pay more and more attention to the health and appearance of pets. Among them, the problem of pet tear stains frequently appears, bringing many troubles to pet raising.

[0003] On the one hand, the reasons for the generation of pet tear stains are relatively complex. From a physiological structure perspective, some pet breeds such as Bichon Frise and Poodle have relatively developed lacrimal glands and secrete relatively more tears, so they are more likely to have tear stains by nature. Moreover, an unreasonable diet structure for pets, such as long-term consumption of high-salt and high-fat foods, will lead to metabolic disorders in the body and promote abnormal increase in tear secretion. Furthermore, when the pet's eyes are infected by bacteria or viruses, or suffer from eye diseases such as conjunctivitis and keratitis, it will also cause inflammatory reactions, stimulate the lacrimal gland, and then secrete a large amount of tears. These excess tears accumulate around the eyes for a long time and form obvious tear stains after oxidation.

[0004] On the other hand, there are many defects in the existing pet tear stain removal solutions. Traditional cleaning methods, such as simply wiping with a wet cloth, can only remove surface stains and cannot deeply solve the root cause of tear stains. Some tear stain removal products on the market have complex chemical components and worrying safety. Some products contain strong irritant cleaners. Long-term use will not only damage the delicate skin around the pet's eyes, but also may cause direct harm to the eyes, triggering discomfort symptoms such as redness, swelling, and itching, and even aggravating the eye condition. There are also some products that claim to have the effect of removing tear stains, but the actual effect is very small and cannot meet the urgent needs of pet owners for effectively removing tear stains.

[0005] In addition, with the enhancement of people's environmental protection awareness, the environmental protection requirements for pet care products are also increasing day by day. Some traditional tear stain removal products have excessive packaging, and a large amount of waste is generated during the production process, which is not conducive to resource conservation and environmental protection. Considering the above factors, there is an urgent need to develop a new preparation that can not only efficiently remove pet tear stains, but also ensure the health of the pet's eyes and has environmental protection characteristics at the same time. Summary of the Invention

[0006] The purpose of the present invention is to provide a preparation for removing pet tear stains to solve the problems raised in the above background art.

[0007] To achieve the above purpose, the present invention provides the following technical solutions:

[0008] A preparation for removing pet tear stains, comprising the following components: vitamin A, vitamin B, vitamin E, vitamin β, lutein, anthocyanin, Ω-3 fatty acid, zinc sulfate, boric acid, levofloxacin, and normal saline with a concentration of 0.9%;

[0009] Among them, based on a dosage of 100 ml, the specific ratio of the components is: 1 mg of vitamin A, 1 mg of vitamin B, 1 mg of vitamin E, 1 mg of vitamin β, 5 mg of lutein, 5 mg of anthocyanin, 1 mg of Ω-3 fatty acid, 24 mg of zinc sulfate, 16 mg of boric acid, 2 mg of levofloxacin, and 100 ml of normal saline with a concentration of 0.9%.

[0010] Preferably, the specific ratio steps of the above components are as follows:

[0011] S1. Apparatus preparation: Prepare a clean glass beaker, glass rod, measuring cylinder, electronic balance, and volumetric flask, and clean and disinfect them to ensure no impurity residue;

[0012] S2. Raw material preparation: Accurately weigh 1 mg of vitamin A, 1 mg of vitamin B, 1 mg of vitamin E, 1 mg of vitamin β, 5 mg of lutein, 5 mg of anthocyanin, 1 mg of Ω-3 fatty acid, 24 mg of zinc sulfate, 16 mg of boric acid, and 2 mg of levofloxacin according to the formula, and at the same time prepare a sufficient amount of normal saline with a concentration of 0.9%;

[0013] S3. Preliminary dissolution: Measure about 80 ml of normal saline with a concentration of 0.9% with a measuring cylinder, pour it into a glass beaker, and sequentially add the weighed vitamin A, vitamin B, vitamin E, vitamin β, lutein, anthocyanin, Ω-3 fatty acid, and zinc sulfate into the beaker, and slowly stir with a glass rod. Observe carefully during the stirring process to ensure that each component is fully dissolved. Since the dissolution rate of some components may be slow, the stirring time can be appropriately extended;

[0014] S4. Add boric acid and dissolve: After the above components are completely dissolved, add the weighed boric acid into the beaker, and continue to stir with a glass rod to completely dissolve the boric acid in the solution. There may be some small particles when boric acid dissolves, and continuous stirring is required until the solution is clear and free of precipitation;

[0015] S5. Add levofloxacin and mix: Then add levofloxacin and stir evenly to ensure that levofloxacin is completely dissolved in the solution;

[0016] S6. Constant volume: Transfer the well - stirred solution to a 100 - ml volumetric flask by means of a glass rod. Then rinse the glass beaker and the glass rod several times with a small amount of 0.9% saline solution, and pour the rinsing liquid into the volumetric flask as well to ensure that all solutes are transferred to the volumetric flask. Then continue to add 0.9% saline solution to the volumetric flask. When the liquid level is close to 1 - 2 cm below the calibration line, use a pipette to add drop by drop until the lowest point of the concave liquid surface of the solution is tangent to the calibration line, thus completing the constant - volume operation;

[0017] S7. Mixing: After constant volume, cover the stopper of the volumetric flask, hold the stopper with the index finger, and support the bottom of the volumetric flask with the other hand. Invert the volumetric flask up and down repeatedly to mix the solution evenly, thus obtaining the preparation for removing pet eye stains.

[0018] Preferably, the vitamin A, vitamin B, vitamin E, and vitamin β all adopt pharmaceutical - grade purity standards, with a purity of not less than 99%, to ensure that each vitamin component can stably play the role of nourishing and repairing pet eye cells at effective doses, and to minimize the potential adverse effects that impurities may bring.

[0019] Preferably, the lutein is extracted from natural plants, mainly including marigold, and is processed by ultrafine grinding to make its particle fineness reach the nanometer level. This can significantly improve the dispersibility of lutein in the preparation, and then enhance its targeted repair effect on the macula area of the pet's eyeball, improving the efficacy of removing eye stains.

[0020] Preferably, the anthocyanin is high - purity anthocyanin, with a proanthocyanidin content of not less than 95%, derived from blueberry extract. By virtue of the various synergistic components naturally rich in blueberries, it strengthens the protection of the pet's eye blood vessels, promotes eye blood circulation, and more efficiently reduces the eye - stain problems caused by poor blood circulation.

[0021] Preferably, the Ω - 3 fatty acid is sourced from deep - sea fish oil and is purified by molecular distillation technology to remove heavy - metal impurities and odor components, ensuring the safety of pet intake. At the same time, it optimizes its stability in the preparation, enabling it to synergistically enhance the effect with other components and stably play the role of slowing down macular degeneration of the eyeball.

[0022] Preferably, the zinc sulfate is in the form of anhydrous zinc sulfate crystals, and needs to be micronized before being added to the preparation, with its particle size between 10 - 50 microns. This helps to accelerate its dissolution rate in the solution, ensuring that it can be quickly and evenly dispersed during the preparation process of the preparation, and fully play the function of promoting the growth and regeneration of retinal cells.

[0023] Preferably, the boric acid is pharmaceutical-grade boric acid. The dissolution process in the preparation needs to be carried out in a constant-temperature water bath environment at 25-30°C. Utilizing the best dissolution characteristics of boric acid in this temperature range can ensure its complete dissolution while avoiding the problem of crystal precipitation caused by too high or too low temperature, and ensuring the stable exertion of the disinfection effect.

[0024] Preferably, the levofloxacin is the levorotatory form, and its optical purity is not less than 98%, so as to enhance its targeted inhibitory ability against eye bacteria and reduce drug side effects. When synergistically inhibiting the eye inflammatory reaction with normal saline, it can more accurately and efficiently reduce the tear secretions.

[0025] Preferably, the 0.9% concentration of normal saline is prepared with medical-grade sodium chloride and is filtered and sterilized through a 0.22-micron microporous membrane after preparation, providing a sterile environment for the preparation, further reducing the risk of pet eye infections caused by microbial contamination, and ensuring the safety and effectiveness of the preparation.

[0026] Compared with the prior art, the present invention provides a preparation for removing pet tear stains, having the following beneficial effects:

[0027] Excellent tear stain removal efficacy: This preparation acts directly on the root cause of tear stains through the synergistic effect of a variety of carefully selected and precisely proportioned ingredients. Vitamins A, B, E, and β start from maintaining the basic physiological functions of the eyes, ensuring the health of the eye epithelial tissue, retina, and cornea, reducing abnormal tear secretion caused by tissue lesions or functional disorders, and laying a solid foundation for tear stain removal; lutein and anthocyanin work together. The former focuses on relieving macular lesions of the eyeball and delaying cataracts, while the latter focuses on promoting the synthesis of visual purple in the eyes and enhancing blood circulation in the eyes. The two jointly improve the overall state of the eyes, effectively curbing excessive tear secretion, and fundamentally reducing tear stains. Ω-3 fatty acids and zinc sulfate act on macular lesions of the eyeball and the growth and regeneration of retinal cells respectively, further optimizing the internal environment of the eyes, reducing increased tears caused by eye discomfort, and achieving a significant tear stain removal effect through multiple measures. After actual testing, after using this preparation for a period of time, the tear stains of the vast majority of pets are significantly faded or even disappeared.

[0028] All-round eye health care: While removing tear stains, each ingredient provides all-round protection for the pet's eyes. Vitamin components continuously nourish eye cells, enhance cell resistance, and resist the invasion of external germs; boric acid and levofloxacin efficiently disinfect and sterilize, forming a strong resistance to common bacterial and viral infections in the eyes, preventing the breeding of eye inflammation, and reducing the risk of tear stains caused by infections; the 0.9% concentration of normal saline creates an osmotic pressure environment similar to that of eye tissues, which is mild and non-irritating. It not only helps other ingredients play their roles but also avoids causing additional burdens to the eyes, comprehensively protecting the health of the pet's eyes and keeping the pet's eyes always bright.

[0029] High safety and mildness: The preparation materials are strictly selected, and all ingredients are strictly screened to ensure that they are non-irritating to the pet's eyes and surrounding skin. High-purity vitamins, lutein and anthocyanins from natural plants, purified omega-3 fatty acids, etc., have eliminated harmful impurities and minimized the risk of adverse reactions such as allergies; medicinal-grade boric acid, levofloxacin, and medical-grade saline strictly follow medical standards and are safe and reliable. The entire preparation process is sophisticated, taking into full consideration the fragile characteristics of pets' eyes. Whether it is used in direct contact or long-term use, it will not cause discomfort symptoms such as redness, itching, and pain in the pet's eyes, allowing pet owners to use it with peace of mind.

[0030] Good stability and ease of use: In formula design, the chemical stability of each component is fully considered, and reasonable matching ensures that the preparation can be stored for a long time at room temperature, is not easy to deteriorate and decompose, and maintains the activity of the effective ingredients. Its appearance is a clear and transparent solution without precipitation or turbidity. When using it, you only need to dip it with simple tools such as cotton balls and gently wipe around the pet's eyes. It is easy to operate and does not require professional skills. Pet owners can easily take care of their pets' eyes at home to solve the problem of tear marks.

[0031] Environmental protection and sustainability advantages: Compared with some traditional pet tear stain removal products, this preparation focuses on the implementation of environmental protection concepts during the research and development process. The packaging adopts a simple design to reduce the waste of resources caused by excessive packaging; the selection of raw materials focuses on sustainability, such as giving priority to natural plant extracts to reduce the negative impact on the environment. While protecting the health of pets, it also contributes to the earth's ecology. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] The present invention provides a preparation for removing tear stains of pets, comprising the following components: vitamin A, vitamin B, vitamin E, vitamin β, lutein, anthocyanin, omega-3 fatty acids, zinc sulfate, boric acid, levofloxacin and 0.9% concentration of physiological saline;

[0034] Among them, based on the dosage of 100 ml, the specific ratio of the components is: vitamin A 1 mg, vitamin B 1 mg, vitamin E 1 mg, vitamin β 1 mg, lutein 5 mg, anthocyanin 5 mg, omega-3 fatty acid 1 mg, zinc sulfate 24 mg, boric acid 16 mg, levofloxacin 2 mg and 0.9% concentration of normal saline 100 ml.

[0035] The specific proportioning steps of the above components are as follows:

[0036] S1. Apparatus preparation: Prepare clean glass beakers, glass rods, measuring cylinders, electronic balances, volumetric flasks, and clean and disinfect them to ensure no impurity residues.

[0037] S2. Raw material preparation: Accurately weigh 1 mg of vitamin A, 1 mg of vitamin B, 1 mg of vitamin E, 1 mg of vitamin β, 5 mg of lutein, 5 mg of anthocyanin, 1 mg of Ω-3 fatty acid, 24 mg of zinc sulfate, 16 mg of boric acid, and 2 mg of levofloxacin according to the formula. At the same time, prepare a sufficient amount of 0.9% concentration physiological saline.

[0038] S3. Preliminary dissolution: Measure about 80 ml of 0.9% concentration physiological saline with a measuring cylinder and pour it into a glass beaker. Then, add the weighed vitamin A, vitamin B, vitamin E, vitamin β, lutein, anthocyanin, Ω-3 fatty acid, and zinc sulfate into the beaker in sequence, and slowly stir with a glass rod. Observe during the stirring process to ensure that each component is fully dissolved. Since the dissolution rate of some components may be slow, the stirring time can be appropriately extended.

[0039] S4. Add boric acid and dissolve: After the above components are completely dissolved, add the weighed boric acid into the beaker and continue to stir with a glass rod to completely dissolve the boric acid in the solution. There may be some small particles when boric acid dissolves, and continuous stirring is required until the solution is clear and free of precipitation.

[0040] S5. Add levofloxacin and mix: Then add levofloxacin and stir evenly to ensure that levofloxacin is completely dissolved in the solution.

[0041] S6. Volume fixation: Transfer the evenly stirred solution to a 100 ml volumetric flask through the drainage of a glass rod. Then, rinse the glass beaker and glass rod with a small amount of 0.9% concentration physiological saline several times, and pour the rinsing solution into the volumetric flask as well to ensure that all solutes are transferred to the volumetric flask. Then, continue to add 0.9% concentration physiological saline to the volumetric flask. When the liquid level is close to 1 - 2 cm below the scale line, use a burette to add drop by drop until the lowest point of the concave liquid surface of the solution is tangent to the scale line to complete the volume fixation operation.

[0042] S7. Shake well: After volume fixation, cover the stopper of the volumetric flask, hold the stopper with the index finger, and support the bottom of the volumetric flask with the other hand. Repeatedly invert it up and down to shake well to make the solution evenly mixed, and thus prepare the preparation for removing pet eye stains.

[0043] Example 1

[0044] Raw material preparation:

[0045] Select vitamins A, B, E, and β with pharmaceutical-grade purity standards and a purity of not less than 99%. Accurately weigh 1 mg of each. These high-purity vitamin raw materials are purchased from professional pharmaceutical suppliers and stored under specified low-temperature and light-proof conditions during storage to ensure their activity.

[0046] Lutein collected from natural marigolds and ultrafinely ground to a nanoscale particle fineness, weigh 5 mg. The ultrafine grinding process uses advanced air-flow grinding equipment and is carried out under low temperature and inert gas protection to avoid lutein oxidation. The prepared nanoscale lutein powder is placed in a sealed and light-proof container for standby.

[0047] Select 5 mg of high-purity anthocyanins with a proanthocyanidin content of not less than 95% and sourced from blueberry extracts. After the blueberries are picked, they are quickly frozen, and the anthocyanins are extracted using a low-temperature countercurrent extraction technique to maximize the retention of co-nutrients in the blueberries, such as vitamin C and vitamin E. The extracted anthocyanins are concentrated and dried to obtain high-purity anthocyanin powder, which is stored in a cool and dry place.

[0048] Prepare 1 mg of Ω-3 fatty acids sourced from deep-sea fish oil purified by molecular distillation technology. After purification, this raw material undergoes strict heavy metal detection to ensure compliance with pet food safety standards. The Ω-3 fatty acids after removing the odor components are stored in a nitrogen-filled sealed package to prevent oxidation.

[0049] Use anhydrous zinc sulfate in crystal form and process it to a particle size between 10 - 50 microns using a micronization device. Weigh 24 mg. The micronized anhydrous zinc sulfate crystals are stored in a dry and moisture-proof container to avoid moisture absorption and caking.

[0050] Select 16 mg of pharmaceutical-grade boric acid and store it in a dry environment to prevent deliquescence.

[0051] Select 2 mg of the levorotatory form of levofloxacin with an optical purity of not less than 98%. Purchase it from a regular pharmaceutical company and store it refrigerated according to the drug storage requirements to ensure the drug efficacy.

[0052] Prepare 100 ml of 0.9% concentration physiological saline made of medical-grade sodium chloride. Immediately after preparation, filter and sterilize it through a 0.22-micron microporous filter membrane, use a sterile package, and store it in a 4 - 10°C and light-proof environment.

[0053] Preparation of the preparation:

[0054] In a laboratory ultra-clean workbench with a cleanliness level of 100,000, use a graduated cylinder to measure approximately 80 ml of pre-sterilized and refrigerated 0.9% concentration physiological saline and pour it into a glass beaker that has been sterilized at high temperature.

[0055] Weigh out high-purity vitamin A, vitamin B, vitamin E, vitamin β, nano-level lutein, high-purity anthocyanin, purified Ω-3 fatty acid, and micronized anhydrous zinc sulfate crystals in sequence and add them to a beaker. Slowly stir with a sterilized glass rod at a stirring speed controlled at 30 - 50 revolutions per minute for 10 - 15 minutes to ensure that each component is fully dissolved;

[0056] Add the weighed pharmaceutical-grade boric acid to the beaker. Place the beaker in a constant temperature water bath environment at 25 - 30 °C and continuously stir at a stirring speed of 100 - 150 revolutions per minute for 5 - 10 minutes to completely dissolve the boric acid and avoid crystallization;

[0057] Then add the refrigerated levo-levofloxacin and continue to stir for 3 - 5 minutes to ensure its complete dissolution in the solution;

[0058] Drain the well-stirred solution through a sterilized glass rod and transfer it to a 100 ml volumetric flask. Then rinse the glass beaker and the glass rod several times with a small amount of pre-sterilized and refrigerated 0.9% concentration physiological saline, and pour the rinsing solution into the volumetric flask as well to ensure that all solutes are transferred to the volumetric flask. Then continue to add physiological saline stored under the same conditions to the volumetric flask. When the liquid level is close to 1 - 2 cm from the calibration line, use a burette to add drop by drop until the lowest point of the concave liquid surface of the solution is tangent to the calibration line to complete the volume-fixing operation;

[0059] After volume-fixing, cover the stopper of the volumetric flask, hold the stopper with the index finger, and support the bottom of the volumetric flask with the other hand. Invert and shake repeatedly up and down to mix the solution evenly, thus obtaining the preparation for removing pet eye stains.

[0060] Quality inspection:

[0061] Conduct an appearance inspection on the prepared preparation. The solution should be clear and transparent, without precipitation, turbidity, or color change;

[0062] Use high-performance liquid chromatography (HPLC) to detect the content of each active ingredient to ensure that the contents of vitamin A, vitamin B, vitamin E, vitamin β, lutein, anthocyanin, Ω-3 fatty acid, zinc sulfate, and levofloxacin are within the specified range, with an error not exceeding ±5%;

[0063] Detect the osmotic pressure of the preparation, which should be close to the osmotic pressure of the pet's eye tissue, with an error range within ±10 mOsm / kg, to ensure that it will not cause irritation to the pet's eyes during use;

[0064] Conduct a sterility test on the preparation and operate according to the sterility test method specified in the Chinese Pharmacopoeia to ensure that the preparation is sterile.

[0065] Example 2

[0066] Raw material adjustment:

[0067] On the basis of Example 1, lutein extracted from natural marigolds of another brand is selected, but it is also required to be ultrafinely ground to the nanoscale, as well as Ω-3 fatty acids from deep-sea fish oil of different origins, to ensure the substitutability and stability of the raw materials.

[0068] For vitamin raw materials, some vitamin derivatives are used, such as vitamin A ester. After converting according to its equivalent activity with vitamin A, the corresponding mass is weighed to explore the influence of different forms of raw materials on the preparation effect.

[0069] Preparation of the preparation:

[0070] Repeat the steps of preparing the preparation in Example 1, and observe the differences in dissolution characteristics and mixing uniformity during the preparation process for different raw material combinations.

[0071] Effect comparison:

[0072] Select two groups of 10 pets each with the same degree of tear stain problems, and use the preparations prepared in Example 1 and Example 2 respectively. Apply once in the morning and once in the evening every day. Dip a cotton ball in the preparation and gently wipe around the pet's eyes, and continue to use for 3 weeks.

[0073] By observing indicators such as the degree of reduction of pet tear stains, the improvement of eye inflammation, and the behavioral responses of pets, compare the actual effects of the two preparations, record and analyze the data, and further optimize the preparation formula and preparation process.

[0074] Example 3

[0075] Process optimization:

[0076] During the preparation process of the preparation, change the stirring tool, use a magnetic stirrer instead of a glass rod for stirring, and adjust the rotation speed and stirring time interval parameters of the magnetic stirrer to study its influence on the dissolution rate of components and the uniformity of the solution.

[0077] For the step of dissolving boric acid in a constant temperature water bath, try to use ultrasonic-assisted dissolution technology. Turn on the ultrasonic device at 25 - 30 °C, set different ultrasonic powers and action times, and observe the dissolution effect of boric acid and its influence on the quality of the preparation.

[0078] Preparation and detection of the preparation:

[0079] Prepare the preparation according to the optimized process, and conduct the same quality inspections as in Example 1, including appearance inspection, component content detection, osmotic pressure detection, and sterility detection.

[0080] Comprehensive evaluation:

[0081] The preparation prepared in Example 3 was comprehensively compared with the preparations of Example 1 and Example 2, and evaluated in terms of preparation efficiency, cost control, quality stability, and actual use effects, etc., to screen out the most optimized preparation process plan, providing a basis for large-scale industrial production.

[0082] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A preparation for removing tear stains from pets, characterized in that: Contains the following ingredients: vitamin A, vitamin B, vitamin E, vitamin beta, lutein, anthocyanin, omega-3 fatty acids, zinc sulfate, boric acid, levofloxacin and 0.9% saline; Among them, based on the dosage of 100 ml, the specific ratio of the components is: vitamin A 1 mg, vitamin B 1 mg, vitamin E 1 mg, vitamin β 1 mg, lutein 5 mg, anthocyanin 5 mg, omega-3 fatty acid 1 mg, zinc sulfate 24 mg, boric acid 16 mg, levofloxacin 2 mg and 0.9% concentration of normal saline 100 ml.

2. A preparation for removing tear stains from pets according to claim 1, characterized in that: The specific proportioning steps of the above components are as follows: S1. Instrument preparation: prepare clean glass beakers, glass rods, measuring cylinders, electronic balances, and volumetric flasks, and clean and sterilize them to ensure that no impurities remain; S2. Raw material preparation: according to the formula, accurately weigh 1 mg of vitamin A, 1 mg of vitamin B, 1 mg of vitamin E, 1 mg of vitamin β, 5 mg of lutein, 5 mg of anthocyanin, 1 mg of omega-3 fatty acid, 24 mg of zinc sulfate, 16 mg of boric acid, and 2 mg of levofloxacin, and prepare a sufficient amount of 0.9% concentration of normal saline; S3. Preliminary dissolution: Use a measuring cylinder to measure about 80 ml of 0.9% saline solution, pour it into a glass beaker, add the weighed vitamin A, vitamin B, vitamin E, vitamin β, lutein, anthocyanin, omega-3 fatty acids, and zinc sulfate into the beaker in turn, stir slowly with a glass rod, pay attention to observe during the stirring process to ensure that each component is fully dissolved. Since some components may dissolve slowly, the stirring time can be appropriately extended; S4. Add boric acid and dissolve: After the above ingredients are completely dissolved, add the weighed boric acid into the beaker and continue to stir with a glass rod to completely dissolve the boric acid in the solution. There may be some small particles when the boric acid dissolves, so continue stirring until the solution is clear without precipitation; S5. Add levofloxacin and mix: Then add levofloxacin and stir evenly to ensure that levofloxacin is completely dissolved in the solution; S6. Volume adjustment: Drain the stirred solution through a glass rod and transfer it to a 100 ml volumetric flask. Rinse the glass beaker and the glass rod several times with a small amount of 0.9% saline solution. Pour the rinse solution into the volumetric flask to ensure that all solutes are transferred to the volumetric flask. Then continue to add 0.9% saline solution to the volumetric flask. When the liquid level is close to the scale line at 1-2 cm, use a rubber-tipped dropper to add dropwise until the lowest point of the concave liquid surface of the solution is tangent to the scale line. The volume adjustment operation is completed. S7. Shake well: After adjusting the volume, cover the bottle stopper of the volumetric flask, hold the stopper with your index finger, and support the bottom of the volumetric flask with your other hand. Invert and shake the flask repeatedly to mix the solution evenly. This is the preparation for removing tear stains from pets.

3. The preparation for removing tear stains from pets according to claim 1, characterized in that: The vitamin A, vitamin B, vitamin E, and vitamin β all adopt pharmaceutical grade purity standards with a purity of not less than 99%, to ensure that each vitamin component can stably play a nourishing and repairing role on the pet's eye cells at an effective dose, and minimize the potential adverse effects that may be caused by impurities.

4. The preparation for removing tear stains from pets according to claim 1, characterized in that: The lutein is extracted from natural plants, mainly including marigold, and is ultra-finely ground to achieve nano-level particle size, which can significantly improve the dispersibility of lutein in the preparation, thereby enhancing its targeted repair effect on the macular area of ​​the pet's eye and improving the efficacy of removing tear marks.

5. The preparation for removing tear stains from pets according to claim 1, characterized in that: The anthocyanidin is a high-purity anthocyanidin with a proanthocyanidin content of not less than 95%. It is derived from blueberry extract. With the help of a variety of synergistic ingredients naturally rich in blueberries, it strengthens the protection of pet's eye blood vessels, promotes eye blood circulation, and more effectively reduces the tear stain problem caused by poor blood circulation.

6. The preparation for removing tear stains from pets according to claim 1, characterized in that: The omega-3 fatty acids are derived from deep-sea fish oil and are purified using molecular distillation technology to remove heavy metal impurities and odor components to ensure safety for pets to ingest. At the same time, their stability in the preparation is optimized so that they can synergize with other ingredients to stably play a role in slowing down macular degeneration of the eye.

7. The preparation for removing tear stains from pets according to claim 1, characterized in that: The zinc sulfate is in the form of anhydrous zinc sulfate crystals and needs to be micronized before being added to the preparation so that its particle size is between 10-50 microns, which helps to accelerate its dissolution rate in the solution, ensures that it can be quickly and evenly dispersed during the preparation process, and fully exerts the function of promoting the growth and regeneration of retinal cells.

8. The preparation for removing tear stains from pets according to claim 1, characterized in that: The boric acid is pharmaceutical grade boric acid, and the dissolution process in the preparation needs to be carried out in a constant temperature water bath environment of 25-30°C, utilizing the optimal solubility characteristics of boric acid in this temperature range to ensure its complete dissolution while avoiding crystallization problems caused by excessively high or low temperatures, thereby ensuring the stable disinfection effect.

9. The preparation for removing tear stains from pets according to claim 1, characterized in that: The levofloxacin is a levorotatory isomer with an optical purity of not less than 98%, so as to enhance its targeted inhibition ability on ocular bacteria and reduce drug side effects. When it cooperates with normal saline to inhibit the inflammatory response of the eye, it can more accurately and efficiently reduce tear secretion.

10. The preparation for removing tear stains from pets according to claim 1, characterized in that: The 0.9% concentration of physiological saline is prepared with medical grade sodium chloride and is sterilized by filtration through a 0.22 micron microporous filter membrane after preparation, thereby providing a sterile environment for the preparation, further reducing the risk of pet eye infection caused by microbial contamination, and ensuring the safety and effectiveness of the preparation.