Composition containing ergothioneine and capable of promoting growth and development as well as preparation method and application of composition

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a composition containing ergothioneine and capable of promoting growth and development as well as a preparation method and application of the composition. The composition containing ergothioneine and capable of promoting growth and development is prepared from the following raw materials: ergothioneine, a spina date seed extract and plant lactobacillus YYY01, the preservation number of the plant lactobacillus YYY01 is GDMCC (China General Microbiological Culture Collection Center) NO: 65147. The mass ratio of the ergothioneine to the spina date seed extract to the plant lactobacillus YYY01 is (10 to 25) to (500 to 600) to 1. The composition containing ergothioneine and capable of promoting growth and development, provided by the invention, has good effects of soothing the nerves and helping sleep and also has a relatively good effect of promoting teenagers to grow tall; and the preparation method is simple and has a good application prospect.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomedicine, and particularly relates to a composition containing ergothioneine and capable of promoting growth and development, a preparation method thereof, and an application thereof. Background Art

[0002] With the improvement of living standards, people's attention to the health of children and adolescents has increased day by day. Growth and development is a crucial stage in childhood and adolescence. Although there are various nutritional supplements on the market, many parents prefer products with fewer side effects. During the growth period, the decisive factor for human height is growth hormone. The secretion of growth hormone is affected by high-protein diet, physical exercise, and stress, but is mainly related to sleep (especially slow-wave sleep). Both slow-wave sleep and paradoxical sleep can promote the secretion of growth hormone, especially slow-wave sleep, which can secrete most of the daily growth hormone in the human body. Most children secrete 50%-70% of the total daily amount within 60-90 minutes after the start of sleep. The initiation of slow-wave sleep is related to 5-HT.

[0003] Currently, drugs for treating sleep disorders mostly act on gamma-aminobutyric acid receptors or dopamine receptors, and can be roughly divided into benzodiazepines, non-benzodiazepines, melatonin, and antidepressant drugs, etc. Promoting bone growth mostly uses growth hormone or nutritional supplements, but most of the above methods will produce certain side effects.

[0004] For example, Chinese Patent CN112022847A provides a composition capable of improving immunity and sleep, a preparation method thereof, and an application thereof. Among them, the composition includes gamma-aminobutyric acid, sodium hyaluronate, and L-ergothioneine. In this composition, the mass ratio of gamma-aminobutyric acid to L-ergothioneine is 1:1-10:1, and the mass ratio of gamma-aminobutyric acid to sodium hyaluronate is 1:5-20:1. The composition provided by this invention has the effects of improving immunity and sleep, and can be widely applied in the fields of food, health products, medicine, etc.

[0005] For example, Chinese Patent CN118620779A relates to the application of Lactiplantibacillus plantarum for relieving anxiety and calming the nerves and helping sleep and its composition in solid beverages. The preservation number of this Lactiplantibacillus plantarum is CGMCC No. 30265. It is isolated from traditional yogurt, has strong safety, no pathogenicity, and no acute toxicity; and after being cultured and screened, through experimental verification, when used in combination with plant extracts, it promotes the production of 5-hydroxytryptamine and enhances the effects of relieving anxiety and calming the nerves and helping sleep.

[0006] Another example is the research on the mechanism of the extract of Semen Ziziphi Spinosae regulating the cAMP-PKA pathway to promote the body length growth of mice and slow-wave sleep in rats [J]. Shanghai Journal of Traditional Chinese Medicine, Xie Yan, Zhang Yunfang, Ning Taoli, etc. It explored the specific mechanism of the extract of Semen Ziziphi Spinosae regulating the cAMP-PKA pathway to affect the body length growth of mice and slow-wave sleep in rats, providing an experimental basis for future research on other drugs promoting slow-wave sleep. For example, it is possible to interfere with slow-wave sleep from multiple targets such as affecting the expression of Gi / o protein and important nodal factors on the cAMP-PKA pathway, such as AC, cAMP, PKA, and 5-HT1A receptor, providing new ideas and research basis for studying drugs promoting bone growth.

[0007] Although the above patents and literatures have certain effects of calming the nerves and helping sleep, their preparation methods are complex, the absorption is slow, and their effects on promoting the growth of teenagers need to be further studied. Therefore, there is an urgent need to study a composition that can improve sleep, has a simple preparation method, and can promote the growth and development of teenagers. Summary of the Invention

[0008] Based on the deficiencies of the prior art, the present invention provides a composition containing ergothioneine and capable of promoting growth and development, its preparation method and application. This composition has a good promoting effect on the height growth of teenagers.

[0009] To achieve the above object, the present invention adopts the following technical solutions:

[0010] On the one hand, the present invention provides a composition containing ergothioneine and capable of promoting growth and development, comprising the following raw materials: ergothioneine, the extract of Semen Ziziphi Spinosae, and Lactiplantibacillus plantarum YYY01; the preservation number of Lactiplantibacillus plantarum YYY01 is GDMCC NO: 65147.

[0011] Preferably, the mass ratio of ergothioneine, the extract of Semen Ziziphi Spinosae, and Lactiplantibacillus plantarum YYY01 is 10-25:500-600:1.

[0012] More preferably, the mass ratio of ergothioneine, the extract of Semen Ziziphi Spinosae, and Lactiplantibacillus plantarum YYY01 is 18-22:500-550:1.

[0013] Even more preferably, the mass ratio of ergothioneine, the extract of Semen Ziziphi Spinosae, and Lactiplantibacillus plantarum YYY01 is 20:500:1.

[0014] On the second hand, the present invention provides a preparation method of the composition containing ergothioneine and capable of promoting growth and development, comprising the following steps: mixing ergothioneine, the extract of Semen Ziziphi Spinosae, and Lactiplantibacillus plantarum YYY01 according to the ratio and stirring evenly to obtain.

[0015] In a third aspect, the present invention provides the use of the above composition containing ergothioneine and capable of promoting growth and development in the preparation of a product having the effect of promoting the growth and development of adolescents or soothing the nerves and helping sleep.

[0016] Specifically, the product includes food, health products or drugs.

[0017] Preferably, the food further includes nutritionally acceptable nutritional additives.

[0018] Preferably, the nutritional additives include, but are not limited to, one or more of dietary fiber, prebiotics, proteins, lipid substances, minerals, and vitamins.

[0019] Preferably, the food includes tablet candies, soy milk, yogurt, canned foods, biscuits, chocolates, pastries, creams, cheeses, milk powders, formula milks, ice creams, jams, purees, candied fruits, preserved fruits, breads, egg rolls, protein drinks, solid drinks, lactic acid bacteria drinks, plant protein drinks, carbonated drinks, coffee or puffed foods.

[0020] In a fourth aspect, the present invention provides the use of the above composition containing ergothioneine and capable of promoting growth and development in milk powder.

[0021] Preferably, the addition amount of the composition containing ergothioneine and capable of promoting growth and development in milk powder is 3 - 5 mg / g, preferably 4 mg / g.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] (1) The composition provided by the present invention has a good effect of soothing the nerves and helping sleep while also having a good effect of promoting the growth of adolescents.

[0024] (2) The preparation method of the composition provided by the present invention is simple and has good application prospects.

[0025] Depositing Instructions

[0026] Deposited strain: Lactiplantibacillus plantarum YYY01;

[0027] Taxonomic name: Lactiplantibacillus plantarum;

[0028] Deposit number: GDMCC N: 65147;

[0029] Deposit date: September 20, 2024;

[0030] Depositary institution: Guangdong Provincial Microbial Culture Collection Center;

[0031] Depository Address: 5th Floor, Building 59, No. 100 Compound, Xianlie Middle Road, Guangzhou City. Detailed Embodiments

[0032] The technical solutions of the present invention will be clearly and completely described below with specific embodiments. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of them. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts fall within the scope of protection of the present invention. The materials, reagents, etc. used, unless otherwise specified, are reagents and materials that can be obtained from commercial sources.

[0033] Ergothioneine was purchased from Barnet Products; wild jujube seed extract was purchased from Nanjing Zelang Biotechnology Co., Ltd.; Lactiplantibacillus plantarum YYY01 was provided by the School of Food Science and Technology, Jiangnan University, with a viable count of 50 billion CFU / g; Lactobacillus rhamnosus LGG was purchased from CHR HANSEN, with a viable count of 50 billion / g. Skim milk powder was purchased from Jiangsu Fuyang Food Co., Ltd.; compound vitamins and compound minerals were purchased from Jin Kangpu Nutrition.

[0034] Example 1 A composition containing ergothioneine and capable of promoting growth and development

[0035] It is composed of the following raw materials: 20 parts of ergothioneine, 500 parts of wild jujube seed extract, and 1 part of Lactiplantibacillus plantarum YYY01.

[0036] Example 2 A composition containing ergothioneine and capable of promoting growth and development

[0037] It is composed of the following raw materials: 10 parts of ergothioneine, 600 parts of wild jujube seed extract, and 1 part of Lactiplantibacillus plantarum YYY01.

[0038] Comparative Example 1 A composition containing ergothioneine and capable of promoting growth and development is composed of the following raw materials:

[0039] It only contains 20 parts of ergothioneine.

[0040] Comparative Example 2 A composition containing ergothioneine and capable of promoting growth and development is composed of the following raw materials:

[0041] It only contains 500 parts of wild jujube seed extract.

[0042] Comparative Example 3 A composition containing ergothioneine and capable of promoting growth and development is composed of the following raw materials:

[0043] It only contains 1 part of Lactiplantibacillus plantarum YYY01.

[0044] Comparative Example 4 A composition containing ergothioneine and capable of promoting growth and development is composed of the following raw materials: 20 parts of ergothioneine and 500 parts of wild jujube seed extract.

[0045] Comparative Example 5 A composition containing ergothioneine and capable of promoting growth and development is composed of the following raw materials: 20 parts of ergothioneine, 500 parts of wild jujube seed extract, and 1 part of Lactobacillus rhamnosus casei.

[0046] Comparative Example 6 A composition containing ergothioneine and capable of promoting growth and development is composed of the following raw materials: 5 parts of ergothioneine, 350 parts of wild jujube seed extract, and 1 part of Lactobacillus plantarum YYY01.

[0047] Mix the compositions obtained in Examples 1-2 and Comparative Examples 1-6 to obtain a composition containing ergothioneine and capable of promoting growth and development.

[0048] Effect experiment

[0049] 1. Experimental method

[0050] Select 54 male weaned rats. After adapting for one week under the conditions of room temperature of 22-25 °C, relative humidity of 50%-60%, normal day and night alternation, and free intake of experimental animal feed and drinking water, the experiment is carried out. The experiment is divided into a normal control group, Examples 1-2 groups, and Comparative Examples 1-6 groups, a total of 9 groups, with 6 rats in each group. Dissolve 100 mg of the compositions prepared in Examples 1-2 groups and Comparative Examples 1-6 groups in 3 ml of drinking water, and gavage once a day. The normal control group is gavaged with 3 ml of drinking water without adding other components. The administration time of the test samples is 42 days. At the end of the experiment, anesthetize with 0.05 mg / g pentobarbital. After the anesthesia is completed, open the chest and abdomen, take blood from the abdominal aorta, centrifuge to obtain serum, and then take out the left femur of the rat.

[0051] 2. Observation indexes

[0052] Body length and weight: The body length and weight of the young rats on the 0th day and the 42nd day of the start of the experiment.

[0053] Femur growth condition: Remove all soft tissues from the obtained left femur of the rat, weigh it with a precision electronic balance, and measure the femur length and the diameter of the middle part of the femur with a vernier caliper.

[0054] Serum indexes: Detect the levels of 5-HT and GH in the collected serum samples strictly according to the instructions of the ELISA kit, and the kit is purchased from Wuhan Ealite Biotechnology Co., Ltd.

[0055] Data processing and result determination

[0056] The data are all expressed as mean ± standard deviation. Statistical analysis is carried out using SPSS 22.0 statistical software. One-way ANOVA is used for comparison among multiple groups. p<0.05 indicates a significant difference between the two, and p<0.01 indicates a very significant difference between the two.

[0057] 3. Experimental Results

[0058] 3.1 Effects on the body weight and body length of rats

[0059] Compared with the normal control group, the body weight and body length of the rats in the example group were significantly increased. Among them, the increases in body length and body weight in Example 1 group were the largest, as shown in Table 1 below.

[0060] Table 1

[0061]

[0062]

[0063] Note: Compared with the normal control group, *p < 0.05, **p < 0.01; different letters indicate significant differences between groups.

[0064] 3.2 Effects of the composition on the femoral growth of rats

[0065] Compared with the normal control group, the femoral bone length in Example 1 group was the longest, the dry weight was the heaviest, and the middle diameter was the longest, as shown in Table 2 below.

[0066] Table 2

[0067]

[0068] Note: Compared with the normal control group, *p < 0.05, **p < 0.01; different letters indicate significant differences between groups.

[0069] 3.3 Effects on 5-HT and growth hormone GH in rat serum

[0070] Example 1 group could significantly increase the contents of 5-HT and GH in rat serum, as shown in Table 3 below.

[0071] Table 3

[0072]

[0073]

[0074] Note: Compared with the normal control group, *p < 0.05, **p < 0.01; different letters indicate significant differences between groups.

[0075] In summary, the composition provided by the present invention has good effects of calming the nerves and promoting bone growth.

[0076] Application Example 1 A milk powder for promoting the growth of teenagers

[0077] The specific components are as shown in Table 4 below:

[0078] Table 4

[0079] Name of raw material or ingredient Usage per ton of powder (kg) Skim milk powder 400 Whole milk powder 400 Lactose 100 Isomaltooligosaccharide 75 Fructooligosaccharide 2 Galactooligosaccharide 2 Milk mineral salts 2 Whey protein powder 1 Compound vitamin 2 Compound mineral 2 Calcium carbonate 10 Composition containing ergothioneine 4

[0080] The above detailed description is a specific description of one of the feasible embodiments of the present invention, and this embodiment is not intended to limit the patent scope of the present invention. It should be noted that all equivalent implementations or changes made without departing from the present invention should be included within the scope of the technical solution of the present invention. Therefore, the protection scope of the present invention shall be subject to the appended claims.

Claims

1. A composition containing ergothioneine and capable of promoting growth and development, characterized in that, The invention comprises the following raw materials: ergothioneine, jujube seed extract and plant lactobacillus YYY01; the preservation number of the plant lactobacillus YYY01 is GDMCC NO: 65147.

2. The composition containing ergothioneine and capable of promoting growth and development according to claim 1, characterized in that, The mass ratio of ergothioneine, the jujube seed extract and Lactobacillus plantarum YYY01 is 10-25:500-600:

1.

3. The composition containing ergothioneine and capable of promoting growth and development according to claim 1, characterized in that, The mass ratio of ergothioneine, the jujube seed extract and Lactobacillus plantarum YYY01 is 20:500:

1.

4. by the preparation method of the composition containing ergothioneine and can promote growth and development described in any one of claims 1-3, it is characterized in that, The method comprises the following steps: mixing ergothioneine, ergothioneine, a jujube seed extract and Lactobacillus plantarum YYY01 according to a proportion, and stirring evenly to obtain the mixture.

5. the application of the composition containing ergothioneine and promoting growth and development described in any one of claim 1-3 or the composition prepared by the preparation method of claim 4 in the product having the effect of promoting the growth and development of adolescents or calming the nerves and helping sleep.

6. The use according to claim 5, characterized in that: The products include food, health products or medicines.

7. The use according to claim 6, characterized in that: The food includes candy flakes, soy milk, yogurt, canned food, biscuits, chocolate, cakes, cream, cheese, milk powder, formula milk powder, ice cream, jam, puree, candied fruit, preserved fruit, bread, egg rolls, protein drinks, solid drinks, lactic acid bacteria drinks, plant protein drinks, carbonated drinks, coffee or puffed food.

8. The use according to claim 6, characterized in that: The food also includes nutritionally acceptable nutritional additives.

9. The use according to claim 8, characterized in that: The nutritional additives include, but are not limited to, one or more of dietary fiber, prebiotics, protein, lipids, minerals, and vitamins.

10. The use according to claim 7, characterized in that: The added amount of the composition containing ergothioneine and capable of promoting growth and development in the milk powder is 3-5 mg / g.

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