Method for extracting selenium-containing flavone from selenium-rich murraya tetragona

Abstract

The invention belongs to the technical field of extraction, and mainly relates to a method for extracting selenium-containing flavone from selenium-rich murraya tetraphylla, which comprises the following steps: (1) pretreatment of fresh leaves and fresh roots of the selenium-rich murraya tetraphylla: removing impurities from the fresh leaves and the fresh roots of the selenium-rich murraya tetraphylla, cleaning, drying in the shade, respectively crushing, spraying deionized water, respectively refrigerating for 2-3 hours, and filtering to obtain filtrate; taking out, soaking in hot water at 40-50 DEG C for 10-20 minutes, taking out, and continuously refrigerating in a low-temperature environment for 1-2 hours; (2) mixing the leaf powder and root powder of the selenium-rich murraya tetraphylla, namely mixing the refrigerated leaf powder and root powder of the selenium-rich murraya tetraphylla according to the weight ratio of 1: (3-4); (3) preparing a selenium-containing flavone extracting solution of the selenium-rich murraya tetraphylla; and (4) extracting the selenium-containing flavone extract of the selenium-rich murraya tetraphylla. According to the method, the selenium-containing flavone in the leaves and the roots of the selenium-rich murraya tetragona can be effectively extracted, and the extraction rate is also remarkably improved.

Description

Technical Field

[0001] The present invention relates to the technical field of extraction, and particularly relates to a method for extracting selenium-containing flavonoids from Murraya tetramera C.C.Huang rich in selenium. Background Art

[0002] Murraya tetramera C.C.Huang, scientifically named Murraya tetramera C.C.Huang, is clearly classified as a plant belonging to the genus Murraya in the Rutaceae family. This classification information is obtained by comprehensively considering various factors such as the morphological characteristics, genetic relationships, and ecological habits of the plant. The Rutaceae family is a family containing various plants, and the genus Murraya is an important genus within it, containing various plants with medicinal and economic values. It is rich in various active ingredients, including aldehydes, phenols, and flavonoids. Currently, there are various methods for extracting its active ingredients.

[0003] Flavonoids refer to a class of compounds with a basic parent nucleus of 2-phenylchromone, which are formed by the mutual connection of two benzene rings (ring A and ring B) with phenolic hydroxyl groups through a central three-carbon atom. Flavonoids have antioxidant and anti-aging effects: Flavonoids are strong antioxidants that can effectively scavenge oxygen free radicals in the body, protect cells from oxidative stress damage, and delay the aging of the human body. Improve blood circulation and cardiovascular health: Flavonoids can improve blood circulation, reduce cholesterol, contain PAF anticoagulant factors, and help prevent cardiovascular and cerebrovascular diseases such as atherosclerosis and improve related symptoms. Anti-inflammatory effect: Flavonoids can inhibit the exudation of inflammatory bioenzymes, reduce the inflammatory response, and have an adjuvant effect on various hypersensitivity and inflammatory diseases such as arthritis and gastroenteritis. Regulate immunity and anti-tumor: Flavonoid compounds can regulate the activity of immune cells, enhance the body's immunity, and at the same time have an inhibitory effect on tumor cells, and can induce apoptosis of tumor cells. Other effects: Flavonoids also have antibacterial and antiviral effects and can be used to prevent and treat infectious diseases. Flavonoids can also promote capillary dilation, accelerate blood flow rate, optimize microcirculation, and ensure that all tissues and organs receive sufficient nutrients and oxygen. In addition, flavonoids have a protective effect on nerve cells and help prevent neurodegenerative diseases. In summary, as a natural compound, flavonoids play multiple positive roles in human health.

[0004] Currently, there are numerous schemes for extracting flavonoids, but no one has studied the efficiency of extracting flavonoids by mixing different parts of Murraya tetramera C.C.Huang rich in selenium. Summary of the Invention

[0005] The invention objective of the present invention is, in view of the above problems, to provide a method for extracting selenium-containing flavonoids from Murraya tetramera C.C.Huang rich in selenium. This method can not only effectively extract selenium-containing flavonoids from the leaves and roots of Murraya tetramera C.C.Huang rich in selenium, but also significantly improve the extraction rate.

[0006] To achieve the above objective, the technical solution adopted by the present invention is:

[0007] A method for extracting selenium-containing flavonoids from Sarcopyramis tetradoxa, comprising the following steps:

[0008] (1) Pretreatment of fresh leaves and fresh roots of Sarcopyramis tetradoxa: Remove impurities from the fresh leaves and fresh roots of Sarcopyramis tetradoxa, wash them, air-dry them, crush them respectively, spray deionized water, and then refrigerate them for 2-3 h respectively. After taking them out, soak them in hot water at 40-50 °C for 10-20 min, and then put them into a low-temperature environment and continue to refrigerate for 1-2 h;

[0009] (2) Mixing of leaf powder and root powder of Sarcopyramis tetradoxa: Mix the refrigerated leaf powder and root powder of Sarcopyramis tetradoxa according to a weight ratio of 1:3-4;

[0010] (3) Selenium-containing flavonoid extract of Sarcopyramis tetradoxa: Add the mixed leaf powder and root powder of Sarcopyramis tetradoxa to an ethyl acetate solution, perform microwave-assisted extraction for 5-10 min, and then continue to extract at room temperature for 5-8 h. Take the filtrate, and repeat the extraction of the filter residue 2-3 times, and combine the filtrates obtained each time;

[0011] (4) Extracting selenium-containing flavonoid extract of Sarcopyramis tetradoxa: Subject the selenium-containing flavonoid extract of Sarcopyramis tetradoxa extracted in step (3) to macroporous resin separation. After elution with an eluent, concentrate and freeze-dry to obtain the selenium-containing flavonoid extract of Sarcopyramis tetradoxa.

[0012] Further explanation: The fresh leaves and fresh roots of Sarcopyramis tetradoxa are air-dried until the moisture content < 10%.

[0013] Further explanation: The amount of deionized water sprayed is 5% of the total mass.

[0014] Preferably, the pretreatment of fresh leaves and fresh roots of Sarcopyramis tetradoxa: Remove impurities from the fresh leaves and fresh roots of Sarcopyramis tetradoxa, wash them, air-dry them, crush them respectively, spray deionized water in an amount of 5% of the total mass, and then refrigerate them for 3 h respectively. After taking them out, soak them in hot water at 50 °C for 20 min, and then put them into a low-temperature environment and continue to refrigerate for 2 h.

[0015] Further explanation: The macroporous resin is macroporous resin AB-8.

[0016] Further explanation: The eluent is an ethanol solution with a concentration of 85%, and the elution rate is 3 BV / h.

[0017] Further explanation: The power of the microwave-assisted extraction is 500 W-600 W.

[0018] Due to the adoption of the above technical solutions, the present invention has the following beneficial effects:

[0019] This application conducts research on the experiment of extracting selenium-containing flavonoids from selenium-rich Murraya tetramera. The research reveals that when the selenium-rich Murraya tetramera leaf powder and root powder are mixed at a specific weight ratio (1:3 to 1:4), the extraction rate of selenium-containing flavonoids can be significantly improved. This discovery indicates that reasonably adjusting the ratio of leaves to roots is crucial for improving the extraction efficiency. When the ratio deviates from this range, whether increasing the proportion of leaves or roots will result in a decrease in the extraction rate, which may be due to the differences in the content of flavonoid compounds and selenium elements in different parts. To further improve the extraction efficiency, this application also carefully explores the pretreatment steps of the raw materials. The experimental results show that spraying deionized water equivalent to 5% of the total mass on the selenium-rich Murraya tetramera leaf powder and root powder first, followed by specific refrigeration (3 hours) and hot water soaking (50°C, 20 minutes), and finally refrigeration again (low-temperature environment, 2 hours), this series of pretreatment measures can significantly improve the extraction rate of selenium-containing flavonoids. This discovery emphasizes the importance of pretreatment in improving extraction efficiency and product quality, which may be achieved through mechanisms such as promoting cell wall rupture and increasing the dissolution rate of active ingredients. In summary, through scientific and rigorous experimental design, this application not only clarifies the optimal mixing ratio of selenium-rich Murraya tetramera leaves and roots, but also innovatively proposes a set of efficient pretreatment processes, providing strong technical support and theoretical basis for the industrial extraction of selenium-containing flavonoid compounds. These findings not only help improve the nutritional value and market competitiveness of related products, but also open up new ways for the development and utilization of selenium-rich plant resources. Detailed implementation manners

[0020] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0021] Embodiment 1:

[0022] A method for extracting selenium-containing flavonoids from selenium-rich Murraya tetramera, comprising the following steps:

[0023] (1) Pretreatment of fresh leaves and fresh roots of selenium-rich Murraya tetramera: Remove impurities and wash the fresh leaves and fresh roots of selenium-rich Murraya tetramera, then air-dry them until the moisture content < 10%. After pulverizing and spraying 5% of the total mass of deionized water respectively, refrigerate them for 2 h, take them out, soak them in hot water at 40°C for 10 min, and then put them into a low-temperature environment and continue to refrigerate for 1 h;

[0024] (2) Mixing of selenium-rich Murraya tetramera leaf powder and root powder: Mix the refrigerated selenium-rich Murraya tetramera leaf powder and root powder according to a weight ratio of 1:3;

[0025] (3) Selenium-rich flavonoid extract of Murraya tetramera: Add the mixed and processed leaf powder and root powder of Murraya tetramera to ethyl acetate solution, perform microwave-assisted extraction at 500 W for 5 min, then continue extraction at room temperature for 5 h. Take the filtrate, and repeat the extraction of the filter residue 2 times, and combine the filtrates obtained each time;

[0026] (4) Extract selenium-rich flavonoid extract of Murraya tetramera: Separate the selenium-rich flavonoid extract of Murraya tetramera obtained in step (3) through macroporous resin AB-8, elute with 85% ethanol solution at an elution rate of 3 BV / h, then concentrate and freeze-dry to obtain the selenium-rich flavonoid extract of Murraya tetramera.

[0027] Example 2:

[0028] A method for extracting selenium-rich flavonoids from Murraya tetramera, comprising the following steps:

[0029] (1) Pretreatment of fresh leaves and fresh roots of selenium-rich Murraya tetramera: Remove impurities and wash the fresh leaves and fresh roots of selenium-rich Murraya tetramera, then air-dry until the moisture content < 10%. Crush them respectively, spray 5% of the total mass of deionized water, then refrigerate for 3 h respectively. After taking them out, soak them in hot water at 50 °C for 20 min, and then put them into a low-temperature environment and continue to refrigerate for 2 h;

[0030] (2) Mixing of leaf powder and root powder of selenium-rich Murraya tetramera: Mix the refrigerated leaf powder and root powder of selenium-rich Murraya tetramera according to a weight ratio of 1:4;

[0031] (3) Selenium-rich flavonoid extract of Murraya tetramera: Add the mixed and processed leaf powder and root powder of Murraya tetramera to ethyl acetate solution, perform microwave-assisted extraction at 600 W for 10 min, then continue extraction at room temperature for 8 h. Take the filtrate, and repeat the extraction of the filter residue 3 times, and combine the filtrates obtained each time;

[0032] (4) Extract selenium-rich flavonoid extract of Murraya tetramera: Separate the selenium-rich flavonoid extract of Murraya tetramera obtained in step (3) through macroporous resin AB-8, elute with 85% ethanol solution at an elution rate of 3 BV / h, then concentrate and freeze-dry to obtain the selenium-rich flavonoid extract of Murraya tetramera.

[0033] Example 3:

[0034] A method for extracting selenium-rich flavonoids from Murraya tetramera, comprising the following steps:

[0035] (1) Pretreatment of fresh leaves and fresh roots of selenium-rich Murraya tetramera: Remove impurities from the fresh leaves and fresh roots of selenium-rich Murraya tetramera, wash them, and air-dry until the moisture content < 10%. After crushing them respectively and spraying 5% of the total mass of deionized water, refrigerate them for 2.5 h respectively. Take them out and soak them in hot water at 45 °C for 15 min, and then put them into a low-temperature environment and continue to refrigerate for 1.5 h;

[0036] (2) Mixing of leaf powder and root powder of selenium-rich Murraya tetramera: Mix the refrigerated leaf powder and root powder of selenium-rich Murraya tetramera according to a weight ratio of 1:3.5;

[0037] (3) Selenium-containing flavone extract of selenium-rich Murraya tetramera: Add the mixed leaf powder and root powder of Murraya tetramera after treatment to an ethyl acetate solution, and perform microwave-assisted extraction at 500W - 600W for 5 - 10 min, and then continue to extract at room temperature for 5 - 8 h. Take the filtrate, and repeat the extraction of the filter residue 2 - 3 times, and combine the filtrates extracted each time;

[0038] (4) Extracting the selenium-containing flavone extract of selenium-rich Murraya tetramera: Separate the selenium-containing flavone extract of selenium-rich Murraya tetramera extracted in step (3) through macroporous resin AB-8, elute it with an 85% ethanol solution at an elution rate of 3 BV / h, and then concentrate and freeze-dry to obtain the selenium-containing flavone extract of selenium-rich Murraya tetramera.

[0039] Test example:

[0040] Experiment 1: Compare the influence of different mixing ratios of leaves and roots of selenium-rich Murraya tetramera on the extraction rate of flavones

[0041] Since different parts are used for extraction and the extraction rates are different, in order to explore the influence of the mixing ratio of leaves and roots on the extraction rate during the extraction process, the applicant preliminarily designed several ratios.

[0042] Group 1: Extract the selenium-containing flavone using the scheme of Example 1.

[0043] Group 2: Extract the selenium-containing flavone using the scheme of Example 2.

[0044] Group 3: It is basically the same as the scheme of Example 1, and the difference is: (2) Mixing of leaf powder and root powder of selenium-rich Murraya tetramera: Mix the refrigerated leaf powder and root powder of selenium-rich Murraya tetramera according to a weight ratio of 1:1.

[0045] Group 4: Extract the selenium-containing flavone using the scheme of Example 1, and the difference is: (2) Mixing of leaf powder and root powder of selenium-rich Murraya tetramera: Mix the refrigerated leaf powder and root powder of selenium-rich Murraya tetramera according to a weight ratio of 1:2.

[0046] Group 5: The selenium-containing flavonoids were extracted using the solution of Example 1, with the difference that: (2) Mixing the leaf powder and root powder of selenium-enriched Murraya tetramera: After refrigeration, the leaf powder and root powder of selenium-enriched Murraya tetramera were mixed at a weight ratio of 1:5.

[0047] The above 5 experimental groups were all experimented with different ratios of the leaf powder and root powder of selenium-enriched Murraya tetramera, and the extraction rates of the selenium-containing flavonoids obtained from the above 5 experimental groups were calculated. The specific results are shown in Table 1.

[0048] Table 1

[0049] Group Leaf powder and root powder / (g: g) Amount of selenium-containing flavonoids / g Extraction rate of selenium-containing flavonoids / % Group 1 150:450 61.24 10.21 Group 2 120:480 60.68 10.11 Group 3 300:300 45.65 7.61 Group 4 200:400 50.21 8.37 Group 5 100:500 51.38 8.56

[0050] From the above extraction rate results, it can be seen that when the leaf powder and root powder of selenium-enriched Murraya tetramera are mixed in a ratio of 1:3 - 4, the selenium-containing flavonoids extracted are high. When the leaf powder and root powder of selenium-enriched Murraya tetramera are mixed in ratios of 1:1, 1:2, and 1:5, the extraction rate of the selenium-containing flavonoids decreases by about 2 - 3%.

[0051] Experiment 2: Comparing the effects of different treatment methods of selenium-enriched Murraya tetramera on the extraction of flavonoids

[0052] In terms of the research experience of the applicant, different raw material treatment methods have an impact on the flavonoid extraction rate. The applicant designed several treatment factors (other steps and parameters are the same as in Example 1) to test the index of the flavonoid extraction rate, which are as follows:

[0053] Table 2 Factor levels

[0054]

[0055] Table 3 Orthogonal experiment results

[0056]

[0057]

[0058] As can be seen from Table 3, the primary and secondary order of the influence of each factor on the flavonoid extraction rate is E > B > A > D > C, that is, the amount of deionized water sprayed accounting for the total mass has the greatest influence on the flavonoid extraction rate, followed by the soaking temperature. The best combination is A3B3C3D3E3, that is, the amount of deionized water sprayed accounts for 5% of the total mass, the refrigeration time is 3 h, the soaking temperature is 50 °C, the soaking time is 20 min, and the continued refrigeration time is 2 h.

[0059] The above description is a detailed description of the preferred and feasible embodiments of the present invention, but the embodiments are not intended to limit the patent application scope of the present invention. Any equivalent changes or modifications completed under the technical concept disclosed by the present invention should fall within the patent scope covered by the present invention.

Claims

1. A method for extracting selenium-containing flavonoids from selenium-rich ophiopogon strychnifolia, characterized in that: The following steps are involved: (1) Pretreatment of fresh leaves and roots of selenium-rich Myrica ovata: remove impurities from the fresh leaves and roots of Myrica ovata, wash them, dry them in the shade, crush them, spray them with deionized water, refrigerate them for 2-3 hours, take them out and soak them in 40-50° C. hot water for 10-20 minutes, take them out and continue to refrigerate them in a low-temperature environment for 1-2 hours; (2) Mixing the selenium-rich leaf powder and root powder of the osmanthus fragrans: Mixing the refrigerated selenium-rich leaf powder and root powder of the osmanthus fragrans at a weight ratio of 1:3-4; (3) Selenium-enriched extract of Selenium-containing flavonoids from Selenium-rich Myrica oleifera: Add the mixed leaf powder and root powder of Selenium oleifera to ethyl acetate solution, perform microwave-assisted extraction for 5-10 minutes, and then continue to extract at room temperature for 5-8 hours, take the filtrate, and then repeat the extraction of the filter residue for 2-3 times, and combine the filtrate of each extraction; (4) Extracting the selenium-containing flavonoid extract of selenium-rich Myrica oleifera: The selenium-containing flavonoid extract of selenium-rich Myrica oleifera extracted in step (3) is separated by a macroporous resin, and after elution with an eluent, the extract is concentrated and freeze-dried to obtain the selenium-containing flavonoid extract of selenium-rich Myrica oleifera.

2. A method for extracting selenium-containing flavonoids from selenium-rich Rhizoma Cibotii according to claim 1, characterized in that: The fresh leaves and fresh roots of the selenium-rich Osmanthus fragrans are shade-dried to a moisture content of less than 10%.

3. A method for extracting selenium-containing flavonoids from selenium-rich ophiopogon odoratus according to claim 1, characterized in that: The amount of the sprayed deionized water is 5% of the total mass.

4. A method for extracting selenium-containing flavonoids from selenium-rich Rhizoma Cibotii according to claim 3, characterized in that: The fresh leaves and roots of the selenium-rich Osmanthus fragrans are pretreated by removing impurities from the fresh leaves and roots of the selenium-rich Osmanthus fragrans, washing them and drying them in the shade, crushing them separately, spraying them with deionized water in an amount of 5% of the total mass, refrigerating them for 3 hours, taking them out and soaking them in 50°C hot water for 20 minutes, and then placing them in a low-temperature environment and continuing to refrigerate them for 2 hours.

5. A method for extracting selenoflavones from selenium-rich Rhizoma Cibotii according to claim 1, characterized in that: The macroporous resin is macroporous resin AB-8.

6. A method for extracting selenium-containing flavonoids from selenium-rich ophiopogon odoratus according to claim 1, characterized in that: The eluent is an ethanol solution with a concentration of 85%, and the elution rate is 3 BV / h.

7. The method for extracting selenoflavones from selenium-rich Rhizoma Cibotii according to claim 1, characterized in that: The power of the microwave-assisted extraction is 500W-600W.

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