Hydrolat crystal rich in linalool and preparation method thereof

Abstract

The invention relates to the technical field of extraction of effective active ingredients of plants, and provides a linalool-rich hydrolat crystal and a preparation method thereof. The method comprises the following steps: S1, crushing plant raw materials, adding the crushed plant raw materials into an ethanol water solution, and extracting to obtain an extracting solution; s2, filtering the extracting solution, performing vacuum concentration under a first temperature condition until no alcohol smell exists, performing distillation under a non-vacuum condition and a second temperature condition, and collecting a condensed aqueous solution to obtain hydrolat, the second temperature being higher than the first temperature; s3, the hydrolat is added into a crystallization solvent for crystallization, and hydrolat crystals are obtained. According to the scheme provided by the invention, the hydrolat crystal rich in linalool is obtained by performing extraction, vacuum concentration, distillation and crystallization on the plant raw material, the prepared hydrolat crystal rich in linalool has strong antibacterial activity, effective components of the plant raw material can be more sufficiently extracted, the production cycle is shortened, and the operation is simple.

Description

Technical Field

[0001] The present invention belongs to the technical field of extraction of effective active ingredients from plants, and particularly relates to a hydrosol crystal rich in linalool and a preparation method thereof. Background Art

[0002] Linalool has an aroma similar to that of lilies and lily of the valley, plays an important role in various fragrance formulations, and also has effects such as antibacterial, antiviral, sedative, anti-anxiety, anti-depressant, insect repellent and insecticidal. Moreover, natural linalool has characteristics such as green, safe, efficient, and long-lasting fragrance. Therefore, the demand for natural linalool is increasing.

[0003] The traditional method for obtaining linalool from plant raw materials is mainly to first obtain the mother liquor by steam distillation, and then perform fractional distillation on the mother liquor to obtain linalool. This method has disadvantages such as high energy consumption, long production cycle, and cumbersome steps. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the present invention provides a hydrosol crystal rich in linalool and a preparation method thereof, aiming to solve the problems mentioned in the background art.

[0005] In a first aspect, the present invention provides a hydrosol crystal rich in linalool and a preparation method thereof, including the following steps:

[0006] Step S1: After crushing the plant raw materials, add them to an ethanol aqueous solution for extraction to obtain an extract;

[0007] Step S2: After filtering the extract, under the first temperature condition, perform vacuum concentration until there is no alcohol smell, and then under non-vacuum and the second temperature condition, perform distillation, collect the condensed aqueous solution to obtain a hydrosol, where the second temperature is greater than the first temperature;

[0008] Step S3: Add the hydrosol to a crystallization solvent for crystallization to obtain a hydrosol crystal.

[0009] Further, in step S1, the plant raw materials are one or two of honeysuckle and basil.

[0010] Further, in step S1, the material-liquid ratio of the plant raw materials to the ethanol aqueous solution is 1 g:5 - 20 mL, and an ethanol aqueous solution with a volume fraction of 40 - 100% is used for extraction.

[0011] Further, in step S1, the hot reflux extraction method is used for extraction, where the extraction temperature is 60 - 100 °C, the stirring speed is 100 - 200 r / min, and the extraction time is 2 - 4 h.

[0012] Further, in step S2, the first temperature is 20-50°C and the second temperature is 80-100°C.

[0013] Further, in step S3, the volume ratio of the hydrosol to the crystallization solvent is 1:1-4, and the crystallization solvent is a mixed solvent of two or more of glycerol, ethanol, isopropanol, n-butanol, methanol, or acetone.

[0014] Further, in step S3, the crystallization solvent is a mixed solvent with a mass ratio of n-butanol:glycerol:methanol = 0.5:1.5:8, methanol:ethanol = 2:8, acetone:isopropanol:ethanol = 1:1:8, n-butanol:acetone:ethanol = 0.5:1:8, n-butanol:acetone:ethanol = 0.5:1:8, or isopropanol:glycerol:methanol = 2:2:6.

[0015] Further, in step S3, the crystallization temperature is 0-4°C and the crystallization time is 12 h.

[0016] On the other hand, the present invention also provides a hydrosol crystal rich in linalool.

[0017] Further, the hydrosol crystal inhibits the growth of Escherichia coli, Candida albicans, or Staphylococcus aureus.

[0018] The present invention has the following technical effects: By extracting, vacuum concentrating, distilling, and crystallizing from plant raw materials, a hydrosol crystal rich in linalool is obtained. The prepared hydrosol crystal rich in linalool has strong antibacterial properties and particularly has a strong inhibitory effect on the growth of Escherichia coli, Candida albicans, and Staphylococcus aureus. This preparation method can more fully extract the effective components of plant raw materials, shorten the production cycle, and is simple to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] By referring to the following drawings, the exemplary embodiments of the present invention can be more fully understood:

[0020] Figure 1 It is a flowchart of the preparation method of the hydrosol crystal rich in linalool provided by the embodiment of the present invention.

[0021] Figure 2 It is the qualitative analysis result of analyzing the hydrosol crystal by the GC-MS method provided by Example 1 of the present invention.

[0022] Figure 3 It is the GC-MS analysis spectrum of linalool provided by Example 1 of the present invention.

[0023] Figure 4 It is the result diagram of the inhibition of Escherichia coli by the hydrosol crystal provided by Example 1 of the present invention, specifically the blank control group.

[0024] Figure 5 This is the inhibition result graph of hydrosol crystals provided in Example 1 of the present invention, specifically the hydrosol crystal group.

[0025] Figure 6 This is the inhibition result graph of hydrosol crystals provided in Example 1 of the present invention against Candida albicans, specifically the blank control group.

[0026] Figure 7 This is the inhibition result graph of hydrosol crystals provided in Example 1 of the present invention against Candida albicans, specifically the hydrosol crystal group.

[0027] Figure 8 This is the inhibition result graph of hydrosol crystals provided in Example 1 of the present invention against Staphylococcus aureus, specifically the blank control group.

[0028] Figure 9 This is the inhibition result graph of hydrosol crystals provided in Example 1 of the present invention against Staphylococcus aureus, specifically the hydrosol crystal group. Detailed implementation manners

[0029] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0031] As Figure 1 shown, in some embodiments, the present invention provides a preparation method of hydrosol crystals rich in linalool, including the following steps:

[0032] Step S1: After crushing the plant raw materials, add them to an ethanol aqueous solution for extraction to obtain an extract.

[0033] Step S2: After filtering the extract, under the first temperature condition, perform reduced pressure concentration until there is no alcohol smell, and then under non-vacuum and the second temperature condition, perform distillation, and collect the condensed aqueous solution to obtain hydrosol, where the second temperature is greater than the first temperature.

[0034] Step S3: Add the hydrosol to a crystallization solvent for crystallization to obtain hydrosol crystals.

[0035] Specifically, in step S1, the plant raw materials are one or two of honeysuckle and basil.

[0036] Specifically, in step S1, the material-liquid ratio of the plant raw material to the aqueous ethanol solution is 1 g: 5-20 mL, and the extraction is carried out using an aqueous ethanol solution with a volume fraction of 40-100%.

[0037] Specifically, in step S1, the extraction is carried out by the hot reflux extraction method, where the extraction temperature is 60-100 °C, the stirring speed is 100-200 r / min, and the extraction time is 2-4 h.

[0038] Specifically, in step S2, the first temperature is 20-50 °C and the second temperature is 80-100 °C.

[0039] Specifically, in step S3, the volume ratio of the hydrosol to the crystallization solvent is 1: 1-4, and the crystallization solvent is a mixed solvent of two or more of glycerol, ethanol, isopropanol, n-butanol, methanol or acetone.

[0040] Specifically, in step S3, the crystallization solvent is a mixed solvent with a mass ratio of n-butanol: glycerol: methanol = 0.5: 1.5: 8, methanol: ethanol = 2: 8, acetone: isopropanol: ethanol = 1: 1: 8, n-butanol: acetone: ethanol = 0.5: 1: 8, n-butanol: acetone: ethanol = 0.5: 1: 8 or isopropanol: glycerol: methanol = 2: 2: 6.

[0041] Specifically, in step S3, the crystallization temperature is 0-4 °C and the crystallization time is 12 h.

[0042] In some embodiments, the present invention also provides a hydrosol crystallization rich in linalool.

[0043] Specifically, the hydrosol crystallization inhibits the growth of Escherichia coli, Candida albicans or Staphylococcus aureus.

[0044] It can be understood that by extracting, concentrating under reduced pressure, distilling and crystallizing from plant raw materials, the obtained hydrosol crystallization rich in linalool has strong antibacterial properties, especially strong inhibitory effects on the growth of Escherichia coli, Candida albicans and Staphylococcus aureus. This preparation method can extract the effective components of plant raw materials more fully, shorten the production cycle and is simple to operate.

[0045] Example 1:

[0046] After crushing 200 g of whole Ocimum basilicum L. plants, add them to an ethanol aqueous solution with a volume fraction of 80%, with a solid-liquid ratio of 1 g:10 mL, and perform thermal reflux extraction. The extraction temperature is 60 °C, the stirring speed is 100 r / min, and the extraction time is 2 h to obtain an extract; after filtering the extract, perform vacuum concentration at 20 °C until there is no alcohol smell, that is, fully remove ethanol, then turn off the vacuum, raise the temperature to 80 °C for distillation, collect the condensed aqueous solution to obtain hydrosol; add the hydrosol to 3 times its volume of a crystallization solvent (n-butanol: glycerol: methanol = 0.5:1.5:8), and perform crystallization at 0 °C for 12 h to obtain hydrosol crystals, which are colorless needle-like crystals. After dehydrating the hydrosol crystals, dissolve them in petroleum ether, and then analyze the chemical components in the hydrosol crystals by GC-MS method, and perform antibacterial experiments on the hydrosol crystals again.

[0047] The qualitative analysis results of the hydrosol crystals analyzed by GC-MS method are as Figure 2 shown. The collection times in the figure at 8.4 min, 9.5 min, 10.2 min, 10.6 min, 10.8 min, 12.2 min, 13.7 min, 14.1 min, 14.8 min, 15.2 min, 16.4 min, and 16.7 min are eucalyptol, linalool, camphor, isoborneol thiocyanoacetate, 4-terpineol, isobornyl acetate, γ-gurjunene, cedrene, β-cubebene, γ-cadinene, cubenol, and cadinol respectively.

[0048] The quantitative analysis results of the hydrosol crystals analyzed by GC-MS method are shown in Table 1. It can be seen that the area proportion of linalool in the hydrosol crystals is 72.15%. The GC-MS analysis chart of linalool is as Figure 3 shown.

[0049] Table 1 Quantitative analysis results of the hydrosol crystals by GC-MS

[0050] Serial number Peak Collection time Area Percentage of area (%) 1 Eucalyptol 8.42 36482.66 1.53 2 Linalool 9.552 1690796.04 72.15 3 Camphor 10.264 26035.56 3.99 4 Isobornyl thiocyanoacetate 10.606 21324.42 3.40 5 4-Terpineol 10.839 63565.19 10.50 6 Isobornyl acetate 12.275 47364.21 8.74 7 γ-Gurjunene 13.711 31640.98 6.40 8 Cedrene 14.1 30680.31 6.63 9 β-Cubebene 14.876 59261.37 13.72 10 γ-Cadinene 15.264 107976.62 28.97 11 Cuparene 16.486 34073.53 12.87 12 Cadinol 16.771 230565.24 9.68

[0051] The experimental results of the antibacterial experiments on the hydrosol crystals are as Figure 4 - 9 shown. Figure 4 - 5 They are the antibacterial effects of the blank control group and the hydrosol crystal group on Escherichia coli respectively, and the antibacterial rate is calculated to be 99.98%; Figure 6 - 7 They are the antibacterial effects of the blank control group and the hydrosol crystal group on Candida albicans respectively, and the antibacterial rate is calculated to be 98.78%; Figure 8 - 9 They are the antibacterial effects of the blank control group and the hydrosol crystal group on Staphylococcus aureus respectively, and the antibacterial rate is calculated to be 99.62%; it can be seen that the hydrosol crystals have strong inhibitory effects on the growth of Escherichia coli, Candida albicans, and Staphylococcus aureus.

[0052] Example 2:

[0053] After crushing 200 g of honeysuckle, it was added to an ethanol aqueous solution with a volume fraction of 40%, the solid-liquid ratio was 1 g:15 mL, and heat reflux extraction was carried out. The extraction temperature was 70 °C, the stirring speed was 150 r / min, and the extraction time was 3 h to obtain an extract. After filtering the extract, vacuum concentration was carried out at 30 °C until there was no alcohol smell, that is, ethanol was fully removed, then the vacuum was closed, the temperature was raised to 90 °C for distillation, and the condensed aqueous solution was collected to obtain hydrosol. The hydrosol was added to 4 times the volume of the crystallization solvent (methanol:ethanol = 2:8), and crystallization was carried out at 2 °C for 12 h to obtain hydrosol crystals, which were colorless needle-shaped crystals. After the hydrosol crystals were dehydrated, they were dissolved in petroleum ether, and then the chemical components in the hydrosol crystals were analyzed by GC-MS method, and the hydrosol crystals were further subjected to an antibacterial experiment.

[0054] Analysis results: The area ratio of linalool in the hydrosol crystals was 64.36%; the antibacterial rate of the hydrosol crystals against Escherichia coli was 97.58%; the antibacterial rate of the hydrosol crystals against Candida albicans was 96.89%; the antibacterial rate of the hydrosol crystals against Staphylococcus aureus was 96.86%.

[0055] Example 3:

[0056] After crushing 200 g of honeysuckle, it was added to an ethanol aqueous solution with a volume fraction of 100%, the solid-liquid ratio was 1 g:20 mL, and heat reflux extraction was carried out. The extraction temperature was 60 °C, the stirring speed was 150 r / min, and the extraction time was 2 h to obtain an extract. After filtering the extract, vacuum concentration was carried out at 50 °C until there was no alcohol smell, that is, ethanol was fully removed, then the vacuum was closed, the temperature was raised to 100 °C for distillation, and the condensed aqueous solution was collected to obtain hydrosol. The hydrosol was added to 1 time the volume of the crystallization solvent (acetone:isopropanol:ethanol = 1:1:8), and crystallization was carried out at 0 °C for 12 h to obtain hydrosol crystals, which were colorless needle-shaped crystals. After the hydrosol crystals were dehydrated, they were dissolved in petroleum ether, and then the chemical components in the hydrosol crystals were analyzed by GC-MS method, and the hydrosol crystals were further subjected to an antibacterial experiment.

[0057] Analysis results: The area ratio of linalool in the hydrosol crystals was 61.42%; the antibacterial rate of the hydrosol crystals against Escherichia coli was 96.85%; the antibacterial rate of the hydrosol crystals against Candida albicans was 95.68%; the antibacterial rate of the hydrosol crystals against Staphylococcus aureus was 96.23%.

[0058] Example 4:

[0059] After crushing 200 g of honeysuckle flowers, add them to an ethanol aqueous solution with a volume fraction of 90%, with a material-liquid ratio of 1 g:10 mL, and carry out hot reflux extraction. The extraction temperature is 60 °C, the stirring speed is 200 r / min, and the extraction time is 4 h to obtain an extract. After filtering the extract, carry out vacuum concentration at 20 °C until there is no alcohol smell, that is, fully remove ethanol, then close the vacuum, raise the temperature to 90 °C for distillation, collect the condensed aqueous solution to obtain hydrosol. Add the hydrosol to 2 times the volume of the crystallization solvent (n-butanol:acetone:ethanol = 0.5:1:8), carry out crystallization at 4 °C, and the crystallization time is 12 h to obtain hydrosol crystals, which are colorless needle-shaped crystals. After dehydrating the hydrosol crystals, dissolve them in petroleum ether, and then use the GC-MS method to analyze the chemical components in the hydrosol crystals, and the hydrosol crystals are further subjected to an antibacterial experiment.

[0060] Analysis results: The area proportion of linalool in the hydrosol crystals is 69.35%; the antibacterial rate of the hydrosol crystals against Escherichia coli is 97.66%; the antibacterial rate of the hydrosol crystals against Candida albicans is 98.42%; the antibacterial rate of the hydrosol crystals against Staphylococcus aureus is 97.61%.

[0061] Example 5:

[0062] After crushing 200 g of Ocimum basilicum whole herb, add it to an ethanol aqueous solution with a volume fraction of 60%, with a material-liquid ratio of 1 g:15 mL, and carry out hot reflux extraction. The extraction temperature is 70 °C, the stirring speed is 150 r / min, and the extraction time is 2 h to obtain an extract. After filtering the extract, carry out vacuum concentration at 30 °C until there is no alcohol smell, that is, fully remove ethanol, then close the vacuum, raise the temperature to 100 °C for distillation, collect the condensed aqueous solution to obtain hydrosol. Add the hydrosol to 2 times the volume of the crystallization solvent (isopropanol:glycerol:methanol = 2:2:6), carry out crystallization at 0 °C, and the crystallization time is 12 h to obtain hydrosol crystals, which are colorless needle-shaped crystals. After dehydrating the hydrosol crystals, dissolve them in petroleum ether, and then use the GC-MS method to analyze the chemical components in the hydrosol crystals, and the hydrosol crystals are further subjected to an antibacterial experiment.

[0063] Analysis results: The area proportion of linalool in the hydrosol crystals is 58.39%; the antibacterial rate of the hydrosol crystals against Escherichia coli is 94.52%; the antibacterial rate of the hydrosol crystals against Candida albicans is 93.68%; the antibacterial rate of the hydrosol crystals against Staphylococcus aureus is 92.69%.

[0064] Example 6:

[0065] After crushing 200 g of whole Ocimum basilicum L. plants, add them to an ethanol aqueous solution with a volume fraction of 40%, with a material-liquid ratio of 1 g:20 mL, and perform thermal reflux extraction. The extraction temperature is 50 °C, the stirring speed is 200 r / min, and the extraction time is 4 h to obtain an extract. After filtering the extract, perform vacuum concentration at 40 °C until there is no alcohol smell, that is, fully remove ethanol, then turn off the vacuum, raise the temperature to 100 °C for distillation, collect the condensed aqueous solution to obtain hydrosol. Add the hydrosol to 3 times its volume of a crystallization solvent (acetone: isopropanol: methanol = 1:1:8), and perform crystallization at 0 °C for 12 h to obtain hydrosol crystals, which are colorless needle-like crystals. After dehydrating the hydrosol crystals, dissolve them in petroleum ether, and then use the GC-MS method to analyze the chemical components in the hydrosol crystals, and the hydrosol crystals are further subjected to an antibacterial experiment.

[0066] Analysis results: The area proportion of linalool in the hydrosol crystals is 52.68%; the antibacterial rate of the hydrosol crystals against Escherichia coli is 90.56%; the antibacterial rate of the hydrosol crystals against Candida albicans is 89.65%; the antibacterial rate of the hydrosol crystals against Staphylococcus aureus is 91.65%.

[0067] Comparative example 1:

[0068] After crushing 200 g of Flos Lonicerae, add them to an ethanol aqueous solution with a volume fraction of 20%, with a material-liquid ratio of 1 g:10 mL, and perform thermal reflux extraction. The extraction temperature is 60 °C, the stirring speed is 200 r / min, and the extraction time is 4 h to obtain an extract. After filtering the extract, perform vacuum concentration at 20 °C until there is no alcohol smell, that is, fully remove ethanol, then turn off the vacuum, raise the temperature to 90 °C for distillation, collect the condensed aqueous solution to obtain hydrosol. Add the hydrosol to 2 times its volume of a crystallization solvent (n-butanol: acetone: ethanol = 0.5:1:8), and perform crystallization at 4 °C for 12 h to obtain hydrosol crystals, which are colorless needle-like crystals. After dehydrating the hydrosol crystals, dissolve them in petroleum ether, and then use the GC-MS method to analyze the chemical components in the hydrosol crystals, and the hydrosol crystals are further subjected to an antibacterial experiment.

[0069] Analysis results: The area proportion of linalool in the hydrosol crystals is 40.22%; the antibacterial rate of the hydrosol crystals against Escherichia coli is 83.26%; the antibacterial rate of the hydrosol crystals against Candida albicans is 85.69%; the antibacterial rate of the hydrosol crystals against Staphylococcus aureus is 82.39%.

[0070] Comparative example 2:

[0071] After crushing 200 g of honeysuckle, it was added to an ethanol aqueous solution with a volume fraction of 40%, the solid-liquid ratio was 1 g:15 mL, and heat reflux extraction was carried out at an extraction temperature of 70 °C, a stirring speed of 150 r / min, and an extraction time of 3 h to obtain an extract; after filtering the extract, vacuum concentration was carried out at 30 °C until there was no alcohol smell, that is, ethanol was completely removed, then the vacuum was turned off, the temperature was raised to 90 °C for distillation, and the condensed aqueous solution was collected to obtain hydrosol; the hydrosol was added to 2 volumes of crystallization solvent (methanol), and crystallization was carried out at 2 °C for 12 h to obtain hydrosol crystals, and the hydrosol crystals were colorless needle-like crystals. After the hydrosol crystals were dehydrated, they were dissolved in petroleum ether, and then the chemical components in the hydrosol crystals were analyzed by GC-MS method, and the hydrosol crystals were further subjected to antibacterial experiments.

[0072] Analysis results: The area percentage of linalool in the hydrosol crystals was 45.69%; the antibacterial rate of the hydrosol crystals against Escherichia coli was 85.21%; the antibacterial rate of the hydrosol crystals against Candida albicans was 86.12%; the antibacterial rate of the hydrosol crystals against Staphylococcus aureus was 84.29%.

[0073] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for preparing linalool-rich hydrosol crystals, characterized in that: The following steps are involved: Step S1: crushing the plant material, adding it into an ethanol aqueous solution for extraction, and obtaining an extract; Step S2: After filtering the extract, the extract is concentrated under reduced pressure at a first temperature until there is no alcohol taste, and then distilled under non-vacuum and second temperature conditions to collect the condensed aqueous solution to obtain a hydrosol, wherein the second temperature is greater than the first temperature; Step S3: adding the hydrosol into a crystallization solvent for crystallization to obtain hydrosol crystals.

2. A method for preparing a linalool-rich hydrosol crystal as claimed in claim 1, characterized in that: In step S1, the plant raw materials are one or both of honeysuckle and basil.

3. A method for preparing a linalool-rich hydrosol crystal as claimed in claim 2, characterized in that: In step S1, the material-liquid ratio of the plant raw material to the ethanol aqueous solution is 1 g:5-20 mL, and the extraction is performed using an ethanol aqueous solution with a volume fraction of 40-100%.

4. A method for preparing a linalool-rich hydrosol crystal as claimed in claim 3, characterized in that: In step S1, hot reflux extraction is used for extraction, wherein the extraction temperature is 60-100° C., the stirring speed is 100-200 r / min, and the extraction time is 2-4 h.

5. A method for preparing a linalool-rich hydrosol crystal as claimed in claim 4, characterized in that: In step S2, the first temperature is 20-50°C, and the second temperature is 80-100°C.

6. A method for preparing a linalool-rich hydrosol crystal as claimed in claim 5, characterized in that: In step S3, the volume ratio of the hydrosol to the crystallization solvent is 1:1-4, and the crystallization solvent is a mixed solvent of two or more of glycerol, ethanol, isopropanol, n-butanol, methanol or acetone.

7. A method for preparing a linalool-rich hydrosol crystal as claimed in claim 6, characterized in that: In step S3, the crystallization solvent is a mixed solvent with a mass ratio of n-butanol: glycerol: methanol = 0.5:1.5:8, methanol: ethanol = 2:8, acetone: isopropanol: ethanol = 1:1:8, n-butanol: acetone: ethanol = 0.5:1:8, n-butanol: acetone: ethanol = 0.5:1:8 or isopropanol: glycerol: methanol = 2:2:

6.

8. A method for preparing a linalool-rich hydrosol crystal as claimed in claim 7, characterized in that: In step S3, the crystallization temperature is 0-4°C and the crystallization time is 12h.

9. A linalool-rich hydrosol crystal, wherein the linalool-rich hydrosol crystal is prepared according to the preparation method according to any one of claims 1 to 8.

10. The linalool-rich hydrosol crystal according to claim 9, characterized in that: Hydrosol crystals inhibit the growth of Escherichia coli, Candida albicans or Staphylococcus aureus.

Images