Preparation method of bio-organic fertilizer

Abstract

The invention provides a preparation method of a bio-organic fertilizer, and belongs to the technical field of bio-organic fertilizers, and the preparation method comprises the following steps: mixing peptone, beef powder, sodium chloride and water, sterilizing to obtain a seed culture medium, inoculating slant strains into the seed culture medium under sterile conditions, and culturing to obtain a seed solution; mixing cane sugar, peptone, sodium chloride and pig manure leaching water, sterilizing to obtain a fermentation medium, and inoculating the seed solution into the fermentation medium for fermentation to obtain a leavening agent; inoculating the leavening agent into a bio-organic fertilizer matrix, and sequentially carrying out anaerobic fermentation and aerobic fermentation to obtain the bio-organic fertilizer. The fermentation agent of the bio-organic fertilizer produced by using the pig manure leaching water as the matrix is applied to the production of the bio-organic fertilizer of the pig manure matrix, and the matrix is homologous, so that the adjustment period of microorganism growth is shortened, the fermentation period of the bio-organic fertilizer is shortened, the production efficiency is improved, and the enterprise efficiency is promoted.

Description

Technical Field

[0001] The invention relates to the technical field of bio-organic fertilizer, and in particular to a preparation method of bio-organic fertilizer. Background Art

[0002] With the continuous development of livestock and poultry farming, the manure and sewage produced by it needs to be utilized as resources, and fertilizer is one of the main ways. With the rise of pig farming industrialization, large-scale pig farms are becoming more and more numerous and larger, and environmental problems are coming along with it. Taking organic nitrogen as an example, chemical fertilizer pollution accounts for 40%, while livestock and poultry manure pollution accounts for 35%, and livestock and poultry manure has become one of the main sources of non-point source pollution in the environment. Pig manure discharge causes huge pollution to the environment and is also a huge waste of resources. If the 400 million tons of pig manure and urine produced throughout the year can be fully utilized, according to the normal amount of fertilizer application, it can solve 1 / 10 of the country's farmland fertilizer use, which will have a great impact on increasing the amount of fertilizer applied in my country's planting industry and reducing the amount of chemical fertilizers. It has become a trend to produce organic fertilizer products using pig farming manure as the main matrix.

[0003] In the production of bio-organic fertilizer products with pig manure as the main matrix, secondary pollutants - pig manure leaching water will be generated. Bio-organic fertilizers (microbial organic fertilizers) refer to a type of fertilizer that combines the effects of microbial fertilizers and organic fertilizers, which are made of specific functional microorganisms and mainly sourced from animal and plant residues and have been harmlessly treated and decomposed (NY884-2012). In the production of bio-organic fertilizers with pig manure as the main matrix, the initial water content of pig manure is generally around 80%. Although 15-30% of straw materials are added during the mixing process, some wastewater will still leach out during the fermentation process, causing secondary pollution. It is necessary to use a sewage treatment system to treat it to meet the secondary emission standards and then discharge it according to regulations.

[0004] Based on this, it is very important to provide a method for preparing a biological organic fertilizer that can take effect faster, thereby improving the fermentation efficiency and shortening the fermentation cycle. Summary of the invention

[0005] The object of the present invention is to provide a method for preparing a bio-organic fertilizer, so as to solve the technical problems of wastewater generation, low fermentation efficiency and long fermentation time in the process of producing the bio-organic fertilizer in the prior art.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The present invention provides a method for preparing a bio-organic fertilizer, comprising the following steps:

[0008] 1) Mix peptone, beef powder, sodium chloride and water, and then sterilize to obtain a seed culture medium. Then, under aseptic conditions, inoculate the slant strain into the seed culture medium for cultivation to obtain a seed solution;

[0009] 2) Mix sucrose, peptone, sodium chloride and leachate of pig manure, and then sterilize to obtain a fermentation culture medium. Then, inoculate the seed solution into the fermentation culture medium for fermentation to obtain a fermenting agent;

[0010] 3) Inoculate the fermenting agent into the biological organic fertilizer matrix and carry out anaerobic fermentation and aerobic fermentation in sequence to obtain biological organic fertilizer.

[0011] Further, the dosage ratio of peptone, beef powder, sodium chloride and water is 8 - 12 g: 1 - 5 g: 3 - 7 g: 1 L.

[0012] Further, the dosage ratio of sucrose, peptone, sodium chloride and leachate of pig manure is 8 - 12 g: 8 - 12 g: 3 - 7 g: 1 L;

[0013] The inoculation amount of the seed solution is 1 - 3% of the fermentation culture medium.

[0014] Further, in the steps 1) and 2), the sterilization temperature is independently 110 - 130 °C, and the sterilization time is independently 20 - 40 min.

[0015] Further, in the step 1), the cultivation temperature is 30 - 40 °C, the cultivation rotation speed is 100 - 200 rpm, and the cultivation time is 18 - 24 min;

[0016] In the step 2), the fermentation temperature is 30 - 40 °C, the fermentation rotation speed is 100 - 200 rpm, and the fermentation time is 18 - 24 min.

[0017] Further, the inoculation amount of the fermenting agent is 1 - 10% of the biological organic fertilizer matrix;

[0018] The biological organic fertilizer matrix includes 80 wt% pig manure and 20 wt% bamboo residues.

[0019] Further, the viable bacteria count of the fermenting agent is 3.2×10 9 ~5.4×10 9 CFU / mL.

[0020] Further, the anaerobic fermentation time is 16 - 20 days, and the aerobic fermentation time is 8 - 12 days.

[0021] Advantages of the present invention:

[0022] 1) On the one hand, the pig manure leachate produced by enterprises in the preparation method of the present invention is utilized in a high-value way, reducing or even eliminating the pressure of wastewater treatment and lowering the operating cost of enterprises; on the other hand, the fermentation agent of bio-organic fertilizer produced with pig manure leachate as the matrix does not need to be purchased externally, reducing the production cost of the bio-organic fertilizer products of enterprises.

[0023] 2) The fermentation agent of bio-organic fertilizer produced with pig manure leachate as the matrix in the preparation method of the present invention is then applied to the production of bio-organic fertilizer with pig manure as the matrix. Due to the same source of the matrix, the adjustment period of microorganisms is shortened, thus shortening the fermentation cycle and improving the production efficiency, which helps enterprises increase efficiency. Specific embodiments

[0024] The present invention provides a preparation method of bio-organic fertilizer, which comprises the following steps:

[0025] 1) Mix peptone, beef powder, sodium chloride and water and sterilize to obtain a seed culture medium, and then inoculate the slant strain into the seed culture medium under sterile conditions for cultivation to obtain a seed solution;

[0026] 2) Mix sucrose, peptone, sodium chloride and pig manure leachate and sterilize to obtain a fermentation culture medium, and then inoculate the seed solution into the fermentation culture medium for fermentation to obtain a fermentation agent;

[0027] 3) Inoculate the fermentation agent into the bio-organic fertilizer matrix and carry out anaerobic fermentation and aerobic fermentation in sequence to obtain bio-organic fertilizer.

[0028] In the present invention, the dosage ratio of peptone, beef powder, sodium chloride and water is 8-12 g: 1-5 g: 3-7 g: 1 L, preferably 9-11 g: 2-4 g: 4-6 g: 1 L, and more preferably 10 g: 3 g: 5 g: 1 L.

[0029] In the present invention, the dosage ratio of sucrose, peptone, sodium chloride and pig manure leachate is 8-12 g: 8-12 g: 3-7 g: 1 L, preferably 9-11 g: 9-11 g: 4-6 g: 1 L, and more preferably 10 g: 10 g: 5 g: 1 L;

[0030] The inoculation amount of the seed solution is 1-3% of the fermentation culture medium, preferably 1.5-2.5%, and more preferably 2%.

[0031] In the present invention, in the steps 1) and 2), the sterilization temperature is independently 110-130 °C, preferably 115-125 °C, and more preferably 118-121 °C; the sterilization time is independently 20-40 min, preferably 25-35 min, and more preferably 30 min.

[0032] In the present invention, in step 1), the cultivation temperature is 30 to 40 °C, preferably 32 to 38 °C, more preferably 34 to 37 °C; the cultivation rotation speed is 100 to 200 rpm, preferably 120 to 180 rpm, more preferably 140 to 160 rpm; the cultivation time is 18 to 24 min, preferably 18.5 to 23.5 min, more preferably 19 to 23 min.

[0033] In the present invention, in step 2), the fermentation temperature is 30 to 40 °C, preferably 32 to 38 °C, more preferably 34 to 37 °C; the fermentation rotation speed is 100 to 200 rpm, preferably 120 to 180 rpm, more preferably 140 to 160 rpm; the fermentation time is 18 to 24 min, preferably 18.5 to 23.5 min, more preferably 19 to 23 min.

[0034] In the present invention, the inoculation amount of the fermenting agent is 1 to 10% of the biological organic fertilizer substrate, preferably 1.5 to 9%, more preferably 2 to 8%;

[0035] The biological organic fertilizer substrate comprises 80 wt% pig manure and 20 wt% bamboo residues.

[0036] In the present invention, the viable count of the fermenting agent is 3.2×10 9 ~5.4×10 9 CFU / mL, preferably 3.3×10 9 ~5.3×10 9 CFU / mL, more preferably 3.4×10 9 ~5.2×10 9 CFU / mL.

[0037] In the present invention, the anaerobic fermentation time is 16 to 20 days, preferably 17 to 19 days, more preferably 18 days; the aerobic fermentation time is 8 to 12 days, preferably 9 to 11 days, more preferably 10 days.

[0038] In the present invention, pig manure leachate refers to the wastewater generated in the production of biological organic fertilizer with pig manure as the main substrate.

[0039] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0040] Example 1

[0041] Mix 10 g of peptone, 3 g of beef extract, 5 g of sodium chloride and 1 L of water, and then sterilize at 121 °C for 30 min to obtain a seed medium. Then, under sterile conditions, inoculate the slant strain into the seed medium and culture at 37 °C and 150 rpm for 18 h to obtain a seed solution;

[0042] Mix 10 g of sucrose, 10 g of peptone, 5 g of sodium chloride and 1 L of leachate of pig manure, and then sterilize at 121 °C for 30 min to obtain a fermentation medium. Then, aseptically add a 1:1 diluted hydrochloric acid solution to adjust the pH value of the medium to 7.0. Inoculate the prepared seed solution into the fermentation medium at an inoculation amount of 2%, and ferment at 37 °C and 150 rpm for 18 h to obtain a fermenter, and the viable count of the fermenter is 3.2×10 9 CFU / mL;

[0043] Inoculate the fermenter into the biological organic fertilizer substrate (80% pig manure + 20% bamboo residue) at an inoculation amount of 2%, and then carry out anaerobic fermentation for 18 days and aerobic fermentation for 10 days in sequence to obtain a biological organic fertilizer, and the quality of the biological organic fertilizer meets the product standard of biological organic fertilizer NY 884-2012.

[0044] Example 2

[0045] Mix 10 g of peptone, 3 g of beef extract, 5 g of sodium chloride and 1 L of water, and then sterilize at 121 °C for 30 min to obtain a seed medium. Then, under sterile conditions, inoculate the slant strain into the seed medium and culture at 37 °C and 150 rpm for 18 h to obtain a seed solution;

[0046] Mix 10 g of sucrose, 10 g of peptone, 5 g of sodium chloride and 1 L of leachate of pig manure, and then sterilize at 121 °C for 30 min to obtain a fermentation medium. Then, aseptically add a 1:1 diluted hydrochloric acid solution to adjust the pH value of the medium to 7.0. Inoculate the prepared seed solution into the fermentation medium at an inoculation amount of 2%, and ferment at 37 °C and 150 rpm for 18 h to obtain a fermenter, and the viable count of the fermenter is 3.2×10 9 CFU / mL;

[0047] Inoculate the fermenter into the biological organic fertilizer substrate (80% pig manure + 20% bamboo residue) at an inoculation amount of 8%, and then carry out anaerobic fermentation for 17 days and aerobic fermentation for 10 days in sequence to obtain a biological organic fertilizer, and the quality of the biological organic fertilizer meets the product standard of biological organic fertilizer NY 884-2012.

[0048] Example 3

[0049] Mix 10 g of peptone, 3 g of beef powder, 5 g of sodium chloride and 1 L of water, then sterilize at 121 °C for 30 min to obtain a seed culture medium. Then, under sterile conditions, inoculate the slant strain into the seed culture medium and culture at 37 °C and 150 rpm for 18 h to obtain a seed solution;

[0050] Mix 10 g of sucrose, 10 g of peptone, 5 g of sodium chloride and 1 L of leachate from pig manure, then sterilize at 121 °C for 30 min to obtain a fermentation medium. Then, aseptically add a 1:1 diluted hydrochloric acid solution to adjust the pH value of the medium to 7.0. Inoculate the prepared seed solution into the fermentation medium at an inoculation amount of 2%, and ferment at 37 °C and 150 rpm for 20 h to obtain a fermenter, and the viable count of the fermenter is 5.2×10 9 CFU / mL;

[0051] Inoculate the fermenter into the biological organic fertilizer substrate (80% pig manure + 20% bamboo residue) at an inoculation amount of 2%, and then carry out anaerobic fermentation for 17 days and aerobic fermentation for 10 days in sequence to obtain biological organic fertilizer, and the quality of the biological organic fertilizer meets the biological organic fertilizer product standard of NY 884-2012.

[0052] Control 1

[0053] Mix 10 g of peptone, 3 g of beef powder, 5 g of sodium chloride and 1 L of water, then sterilize at 121 °C for 30 min to obtain a seed culture medium. Then, under sterile conditions, inoculate the slant strain into the seed culture medium and culture at 37 °C and 150 rpm for 18 h to obtain a seed solution;

[0054] Mix 10 g of sucrose, 10 g of peptone, 5 g of sodium chloride and 1 L of water, then sterilize at 121 °C for 30 min to obtain a fermentation medium. Then, aseptically add a 1:1 diluted hydrochloric acid solution to adjust the pH value of the medium to 7.0. Inoculate the prepared seed solution into the fermentation medium at an inoculation amount of 2%, and ferment at 37 °C and 150 rpm for 20 h to obtain a fermenter, and the viable count of the fermenter is 4.5×10 9 CFU / mL;

[0055] Inoculate the fermenter into the biological organic fertilizer substrate (80% pig manure + 20% bamboo residue) at an inoculation amount of 2%, and then carry out anaerobic fermentation for 20 days and aerobic fermentation for 10 days in sequence to obtain biological organic fertilizer, and the quality of the biological organic fertilizer meets the biological organic fertilizer product standard of NY 884-2012.

[0056] As can be seen from the above embodiments, the present invention provides a method for preparing a biological organic fertilizer, comprising the following steps: mixing peptone, beef powder, sodium chloride and water and then sterilizing to obtain a seed culture medium, and then inoculating a slant strain into the seed culture medium under sterile conditions for culturing to obtain a seed liquid; mixing sucrose, peptone, sodium chloride and pig manure leachate and then sterilizing to obtain a fermentation medium, and then inoculating the seed liquid into the fermentation medium for fermentation to obtain a fermentation agent; inoculating the fermentation agent into a biological organic fertilizer matrix and sequentially performing anaerobic fermentation and aerobic fermentation to obtain a biological organic fertilizer. Compared with Comparative Example 1, the biological organic fertilizer fermentation agent produced by the present invention using pig manure leachate as a matrix is then applied to the production of biological organic fertilizer with a pig manure matrix, reducing the wastewater generated during the production of biological organic fertilizer, and because of the same source of the matrix, shortening the adjustment period of microorganisms, thereby shortening the fermentation cycle and improving the production efficiency, thus helping enterprises increase efficiency.

[0057] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A method for preparing a bio-organic fertilizer, characterized in that: The following steps are involved: 1) Peptone, beef powder, sodium chloride and water are mixed and sterilized to obtain a seed culture medium, and then the slant strain is inoculated into the seed culture medium under sterile conditions for culturing to obtain a seed solution; 2) mixing sucrose, peptone, sodium chloride and pig manure leaching water and sterilizing the mixture to obtain a fermentation medium, and then inoculating the seed liquid into the fermentation medium for fermentation to obtain a starter; 3) The fermentation agent is inoculated into the bio-organic fertilizer matrix to sequentially perform anaerobic fermentation and aerobic fermentation to obtain the bio-organic fertilizer.

2. The preparation method of bio-organic fertilizer according to claim 1, characterized in that, The usage ratio of peptone, beef powder, sodium chloride and water is 8-12g:1-5g:3-7g:1L.

3. The preparation method of bio-organic fertilizer according to claim 2, characterized in that, The dosage ratio of the sucrose, peptone, sodium chloride and pig manure leaching water is 8-12g:8-12g:3-7g:1L; The inoculation amount of the seed liquid is 1-3% of the fermentation medium.

4. The preparation method of the bio-organic fertilizer according to claim 2 or 3, characterized in that, In the steps 1) and 2), the sterilization temperature is independently 110-130° C., and the sterilization time is independently 20-40 minutes.

5. The preparation method of bio-organic fertilizer according to claim 4, characterized in that, In the step 1), the culture temperature is 30-40°C, the culture speed is 100-200 rpm, and the culture time is 18-24 min; In the step 2), the fermentation temperature is 30-40° C., the fermentation speed is 100-200 rpm, and the fermentation time is 18-24 min.

6. The preparation method of the bio-organic fertilizer according to claim 2 or 3 or 5, characterized in that, The inoculation amount of the fermentation agent is 1-10% of the biological organic fertilizer matrix; The bio-organic fertilizer matrix comprises 80 wt % of pig manure and 20 wt % of bamboo residue.

7. The preparation method of bio-organic fertilizer according to claim 6, characterized in that, The viable bacteria count of the fermentation agent was 3.2×10 9 ~5.4×10 9 CFU / mL.

8. The preparation method of bio-organic fertilizer according to claim 7, characterized in that, The anaerobic fermentation time is 16 to 20 days, and the aerobic fermentation time is 8 to 12 days.