Yeast-source bio-organic fertilizer suitable for aquaculture as well as preparation method and application of yeast-source bio-organic fertilizer

Abstract

The invention belongs to the technical field of fertilizers, and particularly relates to a yeast-source bio-organic fertilizer suitable for aquaculture and a preparation method and application of the yeast-source bio-organic fertilizer. The yeast source biological organic fertilizer suitable for aquaculture is prepared from the following components in parts by weight: 40 to 80 parts of yeast metabolite, 15 to 30 parts of ammonium chloride, 10 to 20 parts of monoammonium phosphate and 3 to 8 parts of complex microbial inoculants. The yeast source bio-organic fertilizer suitable for aquaculture is reasonable in proportion, the number of contained beneficial bacteria is large, the multi-bacteria symbiotic environment is good, and the biological activity is high; organic active nutrients, effective microbial populations and inorganic nutrients are complete and scientifically proportioned, so that the fertilizer has a good water fertilizing and algae culturing effect, can improve the culture water environment, supplement bait organisms, promote the growth of cultured objects and increase the product income, and is more beneficial to environmental protection.

Description

Technical Field

[0001] The present invention belongs to the technical field of fertilizers, and particularly relates to a yeast-derived biological organic fertilizer applicable to aquaculture, a preparation method thereof, and an application thereof. Background Art

[0002] As the saying goes, "raising fish starts with raising water". In the field of aquaculture, water conditioning is the key to aquaculture. It is true that the quality of water is related to the success of aquaculture. Good water quality can not only provide sufficient natural bait for fish and shrimp, but also improve the ecological environment of the aquaculture reservoir and promote the healthy growth of fish and shrimp. At present, there are many types of fertilizers applicable to aquaculture on the market, mainly divided into organic fertilizers or inorganic fertilizers. Among them, the inorganic fertilizers have a relatively fast effect on fertilizing water, but excessive use of chemical fertilizers will lead to the accumulation of nutrient salts in the water body, causing water eutrophication, harming the water quality and fish and shrimp, and the maintenance time is not long; while the organic fertilizers have relatively single nutrients. It can be seen that traditional fertilizers can no longer meet the market demand.

[0003] In order to alleviate the above problems, a large number of biological fertilizers containing active microorganisms have emerged in recent years. It is a green and environmentally friendly fertilizer that can not only provide nutrients, but also improve water quality and inhibit the reproduction of harmful bacteria, which has a good promoting effect on the growth of fish and shrimp. However, single microbial inoculants have problems such as single function and poor adaptability. Therefore, it is urgent to prepare a fertilizer containing microbial inoculants that can effectively improve water quality and promote the growth of fish, shrimp and shellfish, so as to meet the requirements of modern fisheries for high yield, high efficiency and greenness. Summary of the Invention

[0004] The technical problem to be solved by the present invention: In the prior art, most of the biological fertilizers applicable to aquaculture are products containing only a single effective bacterium, and the biological activity of the biological bacteria is weak, resulting in low fertilizer efficiency and unable to meet the market demand.

[0005] In view of the above technical problems, the present invention provides a yeast-derived biological organic fertilizer applicable to aquaculture, a preparation method thereof, and an application thereof.

[0006] Specifically, the present invention provides the following technical solutions:

[0007] In the first aspect, the present invention provides a yeast-derived biological organic fertilizer applicable to aquaculture, which comprises, by weight: 40-80 parts of yeast metabolites, 15-30 parts of ammonium chloride, 10-20 parts of monoammonium phosphate, and 3-8 parts of compound microbial inoculum.

[0008] Preferably, by weight, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 40-70 parts of yeast metabolites; and / or, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 15-25 parts of ammonium chloride; and / or, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 10-15 parts of monoammonium phosphate; and / or, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 3-6 parts of compound microbial agent.

[0009] Preferably, the compound microbial agent comprises one or more selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, and Bacillus coagulans.

[0010] Preferably, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens is 3-7:2-6:0.5-3; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus is 3-7:2-6:0.5-3; or, the weight ratio of Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans is 3-7:2-6:0.5-3.

[0011] Preferably, the number of bacteria in the compound microbial agent is 9-11 billion cfu / g; and / or, in the yeast-derived biological organic fertilizer applicable to aquaculture, based on dry basis, the total content of N, P 2 O 5 and K 2 O is more than 10%; and / or, the pH value is 4.5-8.5; and / or, the density is 1.2-1.3 g / cm 3 .

[0012] In a second aspect, the present invention provides a preparation method of a yeast-derived biological organic fertilizer applicable to aquaculture, comprising the following steps:

[0013] Fully mix yeast metabolites, ammonium chloride, monoammonium phosphate, and compound microbial agent to obtain a yeast-derived biological organic fertilizer applicable to aquaculture.

[0014] Preferably, the yeast metabolites are metabolites after fermentation production and extraction of yeast using molasses or hydrolyzed sugar as a culture medium; and / or, based on the weight of the yeast metabolites, the yeast metabolites comprise a total nitrogen content of 1%-5%, a potassium content of 11%-16%, a sodium content of 2%-8%, an organic matter content of 34%-40%, a fulvic acid content of 32%-38%, and / or an amino acid content of 8%-14%.

[0015] In a third aspect, the present invention provides a yeast-derived biological organic fertilizer applicable to aquaculture, which is prepared by the above preparation method of the yeast-derived biological organic fertilizer applicable to aquaculture.

[0016] Fourthly, the present invention provides an application of a yeast-derived bio-organic fertilizer suitable for aquaculture in the preparation of fertilized water.

[0017] Fifthly, the present invention provides an application of a yeast-derived bio-organic fertilizer suitable for aquaculture in one or more of the functions of reducing the ammonia nitrogen content in water, increasing the chlorophyll content in water, and increasing the types and contents of algae.

[0018] Sixthly, the present invention provides a water fertilizing agent, which comprises the above-mentioned yeast-derived bio-organic fertilizer suitable for aquaculture.

[0019] Seventhly, the present invention provides a composition having one or more of the functions of reducing the ammonia nitrogen content in water, increasing the chlorophyll content in water, and increasing the types and contents of algae, and the composition comprises the above-mentioned yeast-derived bio-organic fertilizer suitable for aquaculture.

[0020] Advantages of the present invention

[0021] (1) The ratio of the yeast-derived bio-organic fertilizer suitable for aquaculture in the present invention is reasonable. The number of beneficial bacteria it contains is much higher than that of ordinary bio-bacterial organic fertilizers. The multi-bacteria symbiotic environment is good and the biological activity is strong. Moreover, it is easy to obtain materials, has a low price, has a good fertilizing effect on water, has a high fertilizer efficiency, can greatly increase the yield and income, and is more environmentally friendly.

[0022] (2) The organic matter, effective microbial population and chemical nutrients in the yeast-derived bio-organic fertilizer suitable for aquaculture in the present invention are all complete.

[0023] (3) The yeast-derived bio-organic fertilizer suitable for aquaculture in the present invention can significantly reduce the ammonia nitrogen content in water, has a good effect of fertilizing water and cultivating algae, can improve the aquaculture water environment, supplement bait organisms, promote the growth of aquaculture objects, and increase product income.

[0024] (4) The yeast-derived bio-organic fertilizer suitable for aquaculture in the present invention has been tested by 27 fish, shrimp and shellfish farmers in the Bohai Rim region, including 5 large-scale seawater aquaculture factories. All of them have reported good fertilizing effects on water, which is beneficial to the reduction of aquaculture costs. Detailed implementation manners

[0025] As described above, the purpose of the present invention is to provide a yeast-derived bio-organic fertilizer suitable for aquaculture, its preparation method and application.

[0026] The present invention provides the following technical solutions:

[0027] Technical solution 1. A yeast-derived biological organic fertilizer applicable to aquaculture, calculated by weight, comprises: 40-80 parts of yeast metabolites, 15-30 parts of ammonium chloride, 10-20 parts of monoammonium phosphate, and 3-8 parts of compound bacterial agent.

[0028] Technical solution 2. The yeast-derived biological organic fertilizer applicable to aquaculture according to Technical solution 1, wherein, calculated by weight, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 40-70 parts of yeast metabolites; preferably, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 40-50 parts of yeast metabolites; more preferably, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 40-42 parts of yeast metabolites;

[0029] and / or, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 15-25 parts of ammonium chloride; preferably, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 15-17 parts of ammonium chloride;

[0030] and / or, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 10-15 parts of monoammonium phosphate; preferably, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 10-12 parts of monoammonium phosphate;

[0031] and / or, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 3-6 parts of compound bacterial agent; preferably, the yeast-derived biological organic fertilizer applicable to aquaculture comprises 3-5 parts of compound bacterial agent.

[0032] Technical solution 3. The yeast-derived biological organic fertilizer applicable to aquaculture according to Technical solution 1 or 2, wherein the compound bacterial agent comprises one or more selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus, and Bacillus coagulans;

[0033] Preferably, the compound bacterial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens; or, the compound bacterial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus; or, the compound bacterial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans;

[0034] More preferably, the compound bacterial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens; or, the compound bacterial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans;

[0035] Even more preferably, the compound bacterial agent is Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens.

[0036] Technical solution 4. The yeast-derived biological organic fertilizer applicable to aquaculture according to technical solution 3, wherein the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens is 3-7:2-6:0.5-3; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus laterosporus is 3-7:2-6:0.5-3; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans is 3-7:2-6:0.5-3;

[0037] Preferably, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens is 4.5-6:3.5-5:1-2; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus laterosporus is 4.5-6:3.5-5:1-2; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans is 4.5-6:3.5-5:1-2;

[0038] More preferably, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens is 4.8-5.2:3.5-4:1-1.5; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus laterosporus is 4.8-5.2:3.5-4:1-1.5; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans is 4.8-5.2:3.5-4:1-1.5.

[0039] Technical solution 5. The yeast-derived biological organic fertilizer applicable to aquaculture according to any one of technical solutions 1-4, wherein the number of bacteria in the composite bacterial agent is 9-11 billion cfu / g;

[0040] And / or, in the yeast-derived biological organic fertilizer applicable to aquaculture, based on dry basis, N, P 2 O 5 And K 2 The total content of O is above 10%; and / or, the pH value is 4.5-8.5; and / or, the density is 1.2-1.3 g / cm 3 ;

[0041] Preferably, the total content of N, P 2 O 5 And K 2 The total content of O is 10%-30%; more preferably, the total content of N, P 2 O 5 And K 2 The total content of O is 20%-30%.

[0042] Technical solution 6. A preparation method of the yeast-derived biological organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, comprising the following steps:

[0043] Mix yeast metabolites, ammonium chloride, monoammonium phosphate and compound bacterial agents to obtain a yeast-derived biological organic fertilizer suitable for aquaculture.

[0044] Technical solution 7. The preparation method of the yeast-derived biological organic fertilizer suitable for aquaculture according to Technical solution 6, wherein the yeast metabolites are metabolites after fermentation production and extraction of yeast using molasses or hydrolyzed sugar as a culture medium; preferably, using molasses as the culture medium, inoculate Saccharomyces cerevisiae on the culture medium for fermentation, after fermentation, separate, retain the supernatant for concentration to obtain a yeast fermentation concentrate, and dry it to obtain yeast metabolites; more preferably, the molasses is beet molasses or cane molasses; and / or, more preferably, the pH of the molasses culture medium is 4.5 to 6.0; and / or, more preferably, the fermentation conditions are: the temperature is 25°C to 33°C, and / or the time is 12h to 18h; and / or, more preferably, the dry matter mass percentage in the yeast fermentation concentrate is 45% to 55%; and / or, more preferably, the drying is spray drying; even more preferably, the inlet air temperature is 230 to 260°C and / or the outlet air temperature is 140 to 160°C;

[0045] Technical solution 8. The preparation method of the yeast-derived biological organic fertilizer suitable for aquaculture according to Technical solution 6 or 7, wherein, based on the weight of the yeast metabolites, the yeast metabolites include a total nitrogen content of 1% to 5%, a potassium content of 11% to 16%, a sodium content of 2% to 8%, an organic matter content of 34% to 40%, a fulvic acid content of 32% to 38%, and / or an amino acid content of 8% to 14%.

[0046] Technical solution 9. A yeast-derived biological organic fertilizer suitable for aquaculture, characterized in that the yeast-derived biological organic fertilizer suitable for aquaculture is prepared by the preparation method of the yeast-derived biological organic fertilizer suitable for aquaculture described in any one of Technical solutions 6-8.

[0047] Technical solution 10. Application of the yeast-derived biological organic fertilizer suitable for aquaculture described in any one of Technical solutions 1-5 or the yeast-derived biological organic fertilizer suitable for aquaculture described in Technical solution 9 in the preparation of fertile water;

[0048] and / or, application of the yeast-derived biological organic fertilizer suitable for aquaculture in reducing the ammonia nitrogen content in water;

[0049] and / or, application of the yeast-derived biological organic fertilizer suitable for aquaculture in increasing the chlorophyll content in water;

[0050] and / or, application of the yeast-derived biological organic fertilizer suitable for aquaculture in increasing the types and contents of algae.

[0051] Technical solution 11. A fertilizer and water agent, wherein the fertilizer and water agent comprises the yeast-derived biological organic fertilizer applicable to aquaculture according to any one of Technical solutions 1-5 or the yeast-derived biological organic fertilizer applicable to aquaculture according to Technical solution 9.

[0052] Technical solution 12. A composition having one or more of the effects of reducing the ammonia nitrogen content in water, increasing the chlorophyll content in water, and increasing the types and content of algae, wherein the composition comprises the yeast-derived biological organic fertilizer applicable to aquaculture according to any one of Technical solutions 1-5 or the yeast-derived biological organic fertilizer applicable to aquaculture according to Technical solution 9.

[0053] Technical solution 13. The yeast-derived biological organic fertilizer applicable to aquaculture according to any one of Technical solutions 1-5 or the yeast-derived biological organic fertilizer applicable to aquaculture according to Technical solution 9, wherein the yeast metabolite uses molasses as a culture medium, inoculates Saccharomyces cerevisiae on the culture medium for fermentation, after the fermentation is completed, separates, retains the supernatant for concentration, obtains a yeast fermentation concentrate, and dries it to obtain a yeast metabolite.

[0054] Technical solution 14. The yeast-derived biological organic fertilizer applicable to aquaculture according to any one of Technical solutions 1-5 or the yeast-derived biological organic fertilizer applicable to aquaculture according to Technical solution 9 or 13, wherein the yeast-derived biological organic fertilizer applicable to aquaculture comprises 40 to 80 parts of yeast metabolites. For example, in some embodiments, the yeast-derived biological organic fertilizer comprises 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79 or 80 parts of yeast metabolites, or contains yeast metabolites within the numerical range formed by any two of the above specific numerical values as endpoints.

[0055] Technical solution 15. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of Technical solutions 1-5 or the yeast-derived bio-organic fertilizer applicable to aquaculture according to Technical solutions 9, 13 or 14, wherein the yeast-derived bio-organic fertilizer applicable to aquaculture comprises 15-30 parts of ammonium chloride. For example, in some embodiments, the yeast-derived bio-organic fertilizer comprises 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 parts of ammonium chloride, or ammonium chloride within the numerical range formed by any two of the above specific numerical values as endpoints. The present invention selects ammonium chloride as the nitrogen source considering that, compared with other nitrogen sources, ammonium chloride has a high nitrogen content, good stability, is easy to absorb, and is relatively inexpensive; moreover, when ammonium chloride is used as the nitrogen source in the compounding process with yeast metabolites, the compounded product is not easily hygroscopic and caked, and the product quality stability is good.

[0056] Technical solution 16. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of Technical solutions 1-5 or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of Technical solutions 9 or 13-15, wherein the yeast-derived bio-organic fertilizer applicable to aquaculture comprises 10-20 parts of monoammonium phosphate. For example, in some embodiments, the yeast-derived bio-organic fertilizer comprises 10, 10.5, 11 / 11.5, 12, 12.5, 13, 13.5, 14, 14.5, 15, 15.5, 16, 16.5, 17, 17.5, 18, 18.5, 19, 19.5 or 20 parts of monoammonium phosphate, or monoammonium phosphate within the numerical range formed by any two of the above specific numerical values as endpoints. The monoammonium phosphate selected by the present invention as the phosphorus source is considered because, compared with other phosphorus sources, agricultural-grade monoammonium phosphate has reasonable nitrogen and phosphorus contents, good stability, good water solubility, is easy to absorb, and is relatively inexpensive; moreover, when monoammonium phosphate is used as the phosphorus source in the compounding process with yeast metabolites, the compounded product is not easily hygroscopic and caked, and the product quality stability is good.

[0057] Technical solution 17. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of Technical solutions 1-5 or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of Technical solutions 9 or 13-16, wherein the yeast-derived bio-organic fertilizer applicable to aquaculture comprises 3-8 parts of compound microbial inoculum. For example, in some embodiments, the yeast-derived bio-organic fertilizer comprises 3, 3.2, 3.4, 3.6, 3.8, 4, 4.2, 4.4, 4.6, 4.8, 5, 5.2, 5.4, 5.6, 5.8, 6, 6.2, 6.4, 6.6, 6.8, 7, 7.2, 7.4, 7.6, 7.8 or 8 parts of compound microbial inoculum, or compound microbial inoculum within the numerical range formed by any two of the above specific numerical values as endpoints.

[0058] Technical solution 18. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-17, wherein, by weight, the composite bacterial agent contains 3 to 7 parts of Bacillus subtilis. For example, in some embodiments, the composite bacterial agent includes 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9 or 7 parts of Bacillus subtilis, or contains Bacillus subtilis within the numerical range formed by any two of the above specific numerical values as endpoints.

[0059] Technical solution 19. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-18, wherein, by weight, the composite bacterial agent contains 2 to 6 parts of Bacillus licheniformis. For example, in some embodiments, the composite bacterial agent includes 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9 or 6 parts of Bacillus licheniformis, or contains Bacillus licheniformis within the numerical range formed by any two of the above specific numerical values as endpoints.

[0060] Technical solution 20. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-19, wherein, by weight, the composite bacterial agent contains 0.5 to 3 parts of Bacillus amyloliquefaciens. For example, in some embodiments, the composite bacterial agent includes 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3 parts of Bacillus amyloliquefaciens, or contains Bacillus amyloliquefaciens within the numerical range formed by any two of the above specific numerical values as endpoints.

[0061] Technical solution 21. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5 or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-20, wherein, by weight, the compound bacterial agent contains 0.5 to 3 parts of Bacillus laterosporus. For example, in some embodiments, the compound bacterial agent includes 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3 parts of Bacillus laterosporus, or contains Bacillus laterosporus within the numerical range formed by any two of the above specific values as endpoints.

[0062] Technical solution 22. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5 or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-21, wherein, by weight, the compound bacterial agent contains 0.5 to 3 parts of Bacillus coagulans. For example, in some embodiments, the compound bacterial agent includes 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3 parts of Bacillus coagulans, or contains Bacillus coagulans within the numerical range formed by any two of the above specific values as endpoints.

[0063] Technical solution 23. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5 or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-22, wherein the preparation method of the compound bacterial agent comprises the following steps: first mix Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens to obtain a bacterial strain mixture, and then add zeolite powder and calcium carbonate and mix evenly to obtain the compound bacterial agent.

[0064] Technical solution 24. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5 or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-23, wherein, by weight of the compound bacterial agent, the compound bacterial agent includes 45 to 55 parts of the bacterial strain mixture, 20 to 30 parts of zeolite powder and 20 to 30 parts of calcium carbonate. Further preferably, the compound bacterial agent includes 48 to 52 parts of the bacterial strain mixture, 23 to 27 parts of zeolite powder and 23 to 27 parts of calcium carbonate. More preferably, the compound bacterial agent includes 49 to 51 parts of the bacterial strain mixture, 24 to 26 parts of zeolite powder and 24 to 26 parts of calcium carbonate.

[0065] Technical solution 25. The yeast-derived biological organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5 or the yeast-derived biological organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-24, wherein the zeolite powder refers to the powder obtained by grinding zeolite. Further preferably, any commercially available or self-made zeolite can be used in the present invention to prepare the zeolite powder described in the present invention. Even more preferably, the zeolite used in the zeolite powder includes one or more selected from the group consisting of natrolite, analcime, clinoptilolite, chabazite, heulandite, mordenite, stilbite, laumontite, gismondine and erionite.

[0066] Technical solution 26. Application of the yeast-derived biological organic fertilizer applicable to aquaculture according to technical solution 10 in reducing the ammonia nitrogen content in water, wherein, on the 1st day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 12% - 17%; preferably, on the 1st day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 13% - 15%;

[0067] and / or, on the 2nd day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 16% - 50%; preferably, on the 2nd day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 16% - 20%;

[0068] and / or, on the 3rd day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 25% - 50%; preferably, on the 3rd day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 30% - 32%;

[0069] and / or, on the 4th day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 25% - 80%; preferably, on the 4th day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 55% - 80%; more preferably, on the 4th day after adding the yeast-derived biological organic fertilizer, the removal rate of ammonia nitrogen is 70% - 80%.

[0070] Technical solution 27. Application of the yeast-derived biological organic fertilizer applicable to aquaculture according to technical solution 10 in increasing the chlorophyll content in water, wherein, on the 1st day after adding the yeast-derived biological organic fertilizer, the content of chlorophyll a is 56 - 69 mg / m 3 ; preferably, on the 1st day after adding the yeast-derived biological organic fertilizer, the content of chlorophyll a is 65 - 68 mg / m 3 ;

[0071] and / or, on the 2nd day after adding the yeast-derived biological organic fertilizer, the content of chlorophyll a is 73 - 104 mg / m 3 ; preferably, on the 2nd day after adding the yeast-derived biological organic fertilizer, the content of chlorophyll a is 73 - 100 mg / m3 ;

[0072] and / or, on the 3rd day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 103 - 145 mg / m 3 ; preferably, on the 3rd day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 129 - 145 mg / m 3 ; more preferably, on the 3rd day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 138 - 145 mg / m 3 ;

[0073] and / or, on the 4th day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 125 - 157 mg / m 3 ; preferably, on the 4th day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 138 - 157 mg / m 3 ; more preferably, on the 4th day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 147 - 157 mg / m 3 ;

[0074] and / or, on the 5th day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 105 - 143 mg / m 3 ; preferably, on the 5th day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 125 - 143 mg / m 3 ; more preferably, on the 5th day after adding the yeast-derived bio-organic fertilizer, the content of chlorophyll a is 142 - 143 mg / m 3 .

[0075] Technical solution 28. The application of the yeast-derived bio-organic fertilizer applicable to aquaculture according to Technical solution 10 in improving the types and contents of algae, wherein the algae include one or more selected from the group consisting of Merismopedia sp2, Chroococcus, Chlorococcum, Scenedesmus, Kirchneriella, Cryptomonas, and Cyclotella.

[0076] Technical solution 29. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9 or 13-25. Among them, the present invention does not make any limitation on the source of yeast metabolites, and any commercially available or self-made yeast metabolites can be used in the present invention. Specifically, the inventors of the present invention have found through research that as long as the total nitrogen content of the commercially available or self-made yeast metabolites is ≥1%, the potassium content is ≥11%, the sodium content is ≥2%, the organic matter content is ≥25%, the fulvic acid content is ≥25% and / or the amino acid content is ≥8%, it can be used in the present invention; preferably, the yeast metabolites include a total nitrogen content of 1% to 5%, a potassium content of 11% to 16%, a sodium content of 2% to 8%, an organic matter content of 34% to 40%, a fulvic acid content of 32% to 38%, and / or an amino acid content of 8% to 14%; more preferably, the yeast metabolites include a total nitrogen content of 2.6% to 3.6%, a potassium content of 13.5% to 14.5%, a sodium content of 5% to 6%, an organic matter content of 36.7% to 37.7%, a fulvic acid content of 35% to 36%, and / or an amino acid content of 10.7% to 11.7%; most preferably, the yeast metabolites include a total nitrogen content of 3.19%, a potassium content of 13.92%, a sodium content of 5.78%, an organic matter content of 37.2%, a fulvic acid content of 35.36%, and / or an amino acid content of 11.2%.

[0077] Technical solution 30. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9, 29 or 13-25. Among them, the present invention does not make any limitation on the source of Bacillus subtilis, and any commercially available or self-made Bacillus subtilis can be used in the present invention; preferably, the inventors of the present invention have found through research that as long as the viable count of the commercially available or self-made Bacillus subtilis is ≥15 billion CFU / g, it can be used in the present invention, and the viable count is preferably 19 billion to 25 billion CFU / g, more preferably 19 billion to 21 billion CFU / g, further preferably 19.5 billion to 20.5 billion CFU / g, and more preferably 19.9 billion to 20.1 billion CFU / g.

[0078] Technical solution 31. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9, 29, 30, or 13-25. Among them, the present invention does not make any limitation on the source of Bacillus licheniformis, and any commercially available or self-made Bacillus licheniformis can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of the commercially available or self-made Bacillus licheniformis is ≥ 15 billion CFU / g, it can be used in the present invention. The viable count is preferably 19 billion - 25 billion CFU / g, more preferably 19 billion - 21 billion CFU / g, further preferably 19.5 billion - 20.5 billion CFU / g, and even more preferably 19.9 billion - 20.1 billion CFU / g.

[0079] Technical solution 32. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9, 29-31, or 13-25. Among them, the present invention does not make any limitation on the source of Bacillus laterosporus, and any commercially available or self-made Bacillus laterosporus can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of the commercially available or self-made Bacillus laterosporus is ≥ 15 billion CFU / g, it can be used in the present invention. The viable count is preferably 19 billion - 25 billion CFU / g, more preferably 19 billion - 21 billion CFU / g, further preferably 19.5 billion - 20.5 billion CFU / g, and even more preferably 19.9 billion - 20.1 billion CFU / g.

[0080] Technical solution 33. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9, 29-32, or 13-25. Among them, the present invention does not make any limitation on the source of Bacillus coagulans, and any commercially available or self-made Bacillus coagulans can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of the commercially available or self-made Bacillus coagulans is ≥ 15 billion CFU / g, it can be used in the present invention. The viable count is preferably 19 billion - 25 billion CFU / g, more preferably 19 billion - 21 billion CFU / g, further preferably 19.5 billion - 20.5 billion CFU / g, and even more preferably 19.9 billion - 20.1 billion CFU / g.

[0081] Technical solution 34. The yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 1-5, or the yeast-derived bio-organic fertilizer applicable to aquaculture according to any one of technical solutions 9, 29-33, or any one of 13-25. Among them, the present invention does not make any limitation on the source of Bacillus coagulans, and any commercially available or self-made Bacillus amyloliquefaciens can be used in the present invention; preferably, the inventors have found through research that as long as the viable count of the commercially available or self-made Bacillus amyloliquefaciens ≥ 15 billion CFU / g, it can be used in the present invention, and the viable count is preferably 19-25 billion CFU / g, more preferably 19-21 billion CFU / g, further preferably 19.5-20.5 billion CFU / g, and more preferably 19.9-20.1 billion CFU / g.

[0082] All kinds of reagents / instruments used in the examples and comparative examples of the present invention are conventional commercially available products unless otherwise specified. The sources of the experimental materials used in the present invention are shown in Table 1 below.

[0083] Table 1 Experimental materials and suppliers

[0084]

[0085] Among them, in the present invention, the detection method of total nitrogen content refers to the 3.1 distillation and titration method (arbitration method) in the determination of total nitrogen, phosphorus and potassium content of water-soluble fertilizers in the agricultural industry standard NY / T1977-2010 of the People's Republic of China. The detection method of potassium content refers to the determination of potassium content in the determination of total nitrogen, phosphorus and potassium content of water-soluble fertilizers in the agricultural industry standard NY / T1977-2010 of the People's Republic of China, the potassium tetraphenylborate gravimetric method. The detection method of phosphorus content refers to the determination of phosphorus content in the determination of total nitrogen, phosphorus and potassium content of water-soluble fertilizers in the agricultural industry standard NY / T1977-2010 of the People's Republic of China, the phosphomolybdic acid quinoline gravimetric method. The detection method of sodium content refers to the 3.1 flame photometry in the determination of sodium, selenium and silicon content of water-soluble fertilizers in the agricultural industry standard NY / T1972-2010 of the People's Republic of China. The detection method of organic matter content refers to the determination of organic matter content of water-soluble fertilizers in the agricultural industry standard NY / T1976-2010 of the People's Republic of China. The detection method of fulvic acid content refers to the determination of humic acid (total humic acid or soluble humic acid) and fulvic acid content (volumetric method) in the analysis method of ammonium humate fertilizer in the chemical industry standard HG / T 3276-2019 of the People's Republic of China. The detection method of amino acid content refers to the 3 conventional acid hydrolysis method in the determination of amino acids in feed in the national standard GB / T 18246-2019 of the People's Republic of China.

[0086] In order to better understand the technical solution of the present invention, the technical solution of the present invention will be described in detail below with specific examples.

[0087] Example 1

[0088] (1) Formula of yeast-derived bio-organic fertilizer applicable to aquaculture

[0089] A yeast-derived bio-organic fertilizer applicable to aquaculture, calculated by weight, includes 60 parts of yeast metabolites, 22 parts of ammonium chloride, 14 parts of monoammonium phosphate, and 4 parts of compound microbial agents. Among them, the compound microbial agents contain Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens with a mass ratio of 5:4:1. The viable count in the compound microbial agents is 10 billion CFU / g.

[0090] Among them, the preparation method of the compound microbial agents includes the following steps: first, mix Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens according to a mass ratio of 5:4:1 to obtain a strain mixture, and then add zeolite powder and calcium carbonate and mix evenly to obtain the compound microbial agents; among them, calculated by the total mass of the compound microbial agents, the compound microbial agents include 50% strain mixture, 25% zeolite powder, and 25% calcium carbonate.

[0091] Among them, the yeast metabolites are purchased from Angel Yeast (Binzhou) Co., Ltd. The yeast metabolites include: calculated by the weight of the yeast metabolites, the total nitrogen content is 3.19%, the potassium content is 13.92%, the sodium content is 5.78%, the organic matter content is 37.2%, the fulvic acid content is 35.36%, and the amino acid content is 11.2%.

[0092] (2) Preparation method of yeast-derived bio-organic fertilizer applicable to aquaculture

[0093] A preparation method of a yeast-derived bio-organic fertilizer applicable to aquaculture includes the following steps:

[0094] Send the yeast metabolites into the feeding pipeline, and at the same time, send the ammonium chloride, monoammonium phosphate, and compound microbial agents that have been added to the feeding bin of the auxiliary material adding system into the feeding pipeline according to the formula ratio of the yeast-derived bio-organic fertilizer applicable to aquaculture. Adjust the feeding flow rates of the yeast metabolites, ammonium chloride, monoammonium phosphate, and compound microbial agents through their respective flow control valves to make them fully mixed evenly in the material sample collection pipeline. The evenly mixed material samples gather in the packaging bin and are discharged through the lower port for sub-packaging.

[0095] The product indexes of the prepared yeast-derived bio-organic fertilizer applicable to aquaculture: the total nutrient (the sum of N, P 2 O 5 and K 2 O) (calculated based on the dry basis of the organic fertilizer) is 26%; the moisture content is 0.32%; the pH is 6.6; the density is 1.25 g / cm 3 .

[0096] Example 2

[0097] (1) Formula of a yeast-derived biological organic fertilizer suitable for aquaculture

[0098] A yeast-derived biological organic fertilizer suitable for aquaculture, by weight, comprises 40 parts of yeast metabolites, 15 parts of ammonium chloride, 10 parts of monoammonium phosphate and 3 parts of compound microbial agents. Among them, the compound microbial agents contain Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens with a mass ratio of 5:4:1. The viable count in the compound microbial agents is 10 billion CFU / g.

[0099] Among them, the preparation method of the compound microbial agents is the same as that of the compound microbial agents in Example 1. The yeast metabolites are the same as those in Example 1.

[0100] (2) The preparation method of the yeast-derived biological organic fertilizer suitable for aquaculture is the same as that of the yeast-derived biological organic fertilizer suitable for aquaculture in Example 1.

[0101] Product indexes of the prepared yeast-derived biological organic fertilizer suitable for aquaculture: total nutrients (sum of N, P 2 O 5 and K 2 O) (calculated based on the dry weight of the organic fertilizer) is 25%; moisture content is 0.43%; pH is 5.9; density is 1.28 g / cm 3 .

[0102] Example 3

[0103] (1) Formula of a yeast-derived biological organic fertilizer suitable for aquaculture

[0104] A yeast-derived biological organic fertilizer suitable for aquaculture, by weight, comprises 80 parts of yeast metabolites, 30 parts of ammonium chloride, 20 parts of monoammonium phosphate and 8 parts of compound microbial agents. Among them, the compound microbial agents contain Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens with a mass ratio of 5:4:1. The viable count in the compound microbial agents is 10 billion CFU / g.

[0105] Among them, the preparation method of the compound microbial agents is the same as that of the compound microbial agents in Example 1. The yeast metabolites are the same as those in Example 1.

[0106] (2) The preparation method of the yeast-derived biological organic fertilizer suitable for aquaculture is the same as that of the yeast-derived biological organic fertilizer suitable for aquaculture in Example 1.

[0107] Product indexes of the prepared yeast-derived biological organic fertilizer suitable for aquaculture: total nutrients (sum of N, P 2 O 5 and K 2The total content of N, P, and K (calculated based on the dry weight of the organic fertilizer) is 27%; the moisture content is 0.49%; the pH is 6.9; the density is 1.26 g / cm 3 .

[0108] Example 4

[0109] (1) Formula of a yeast-derived biological organic fertilizer applicable to aquaculture

[0110] A yeast-derived biological organic fertilizer applicable to aquaculture, by weight, comprises 60 parts of yeast metabolites, 25 parts of ammonium chloride, 10 parts of monoammonium phosphate, and 5 parts of compound microbial agents. Among them, the compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 6:5:2. The viable count in the compound microbial agent is 10 billion CFU / g.

[0111] Among them, the preparation method of the compound microbial agent includes the following steps: First, mix Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 6:5:2 to obtain a strain mixture, and then add zeolite powder and calcium carbonate and mix evenly to obtain the compound microbial agent; among them, based on the total mass of the compound microbial agent, the compound microbial agent includes 50% of the strain mixture, 25% of zeolite powder, and 25% of calcium carbonate.

[0112] Among them, the yeast metabolites are the same as those in Example 1.

[0113] (2) The preparation method of the yeast-derived biological organic fertilizer applicable to aquaculture is the same as that of the yeast-derived biological organic fertilizer applicable to aquaculture in Example 1.

[0114] The product indexes of the prepared yeast-derived biological organic fertilizer applicable to aquaculture: total nutrients (N, P 2 O 5 and K 2 The total content of O (calculated based on the dry weight of the organic fertilizer) is 23%; the moisture content is 0.35%; the pH is 7; the density is 1.24 g / cm 3 .

[0115] Example 5

[0116] (1) Formula of a yeast-derived biological organic fertilizer applicable to aquaculture

[0117] A yeast-derived biological organic fertilizer applicable to aquaculture, by weight, comprises 42 parts of yeast metabolites, 30 parts of ammonium chloride, 20 parts of monoammonium phosphate, and 8 parts of compound microbial agents. Among them, the compound microbial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 4:3:1. The viable count in the compound microbial agent is 10 billion CFU / g.

[0118] Among them, the preparation method of the composite bacterial agent includes the following steps: First, mix Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloliquefaciens in a mass ratio of 4:3:1 to obtain a bacterial strain mixture, and then add zeolite powder and calcium carbonate and mix evenly to obtain the composite bacterial agent; among them, based on the total mass of the composite bacterial agent, the composite bacterial agent includes 50% of the bacterial strain mixture, 25% of zeolite powder, and 25% of calcium carbonate.

[0119] Among them, the yeast metabolite is the same as the yeast metabolite in Example 1.

[0120] (2) The preparation method of the yeast-derived bio-organic fertilizer applicable to aquaculture is the same as the preparation method of the yeast-derived bio-organic fertilizer applicable to aquaculture in Example 1.

[0121] Product indicators of the prepared yeast-derived bio-organic fertilizer applicable to aquaculture: The total nutrients (the sum of N, P 2 O 5 and K 2 O) (calculated based on the dry weight of the organic fertilizer) is 29%; the moisture content is 0.37%; the pH is 6.2; the density is 1.26 g / cm 3 .

[0122] Example 6

[0123] (1) Formula of a yeast-derived bio-organic fertilizer applicable to aquaculture

[0124] A yeast-derived bio-organic fertilizer applicable to aquaculture, in parts by weight, includes 60 parts of yeast metabolite, 22 parts of ammonium chloride, 14 parts of monoammonium phosphate, and 4 parts of composite bacterial agent. Among them, the composite bacterial agent contains Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus in a mass ratio of 5:3.5:1.5. The viable bacteria count in the composite bacterial agent is 100 billion CFU / g.

[0125] Among them, the preparation method of the composite bacterial agent includes the following steps: First, mix Bacillus subtilis, Bacillus licheniformis, and Bacillus laterosporus in a mass ratio of 5:3.5:1.5 to obtain a bacterial strain mixture, and then add zeolite powder and calcium carbonate and mix evenly to obtain the composite bacterial agent; among them, based on the total mass of the composite bacterial agent, the composite bacterial agent includes 50% of the bacterial strain mixture, 25% of zeolite powder, and 25% of calcium carbonate.

[0126] Among them, the yeast metabolite is the same as the yeast metabolite in Example 1.

[0127] (2) The preparation method of the yeast-derived bio-organic fertilizer applicable to aquaculture is the same as the preparation method of the yeast-derived bio-organic fertilizer applicable to aquaculture in Example 1.

[0128] Product indicators of the yeast-derived bio-organic fertilizer suitable for aquaculture: Total nutrients (sum of N, P 2 O 5 and K 2 O) (calculated based on the dry weight of the organic fertilizer) is 26%; moisture content is 0.29%; pH is 6.5; density is 1.25 g / cm 3 .

[0129] Example 7

[0130] (1) Formula of a yeast-derived bio-organic fertilizer suitable for aquaculture

[0131] A yeast-derived bio-organic fertilizer suitable for aquaculture, by weight, comprises 40 parts of yeast metabolites, 15 parts of ammonium chloride, 10 parts of monoammonium phosphate, and 3 parts of compound microbial agents. Among them, the compound microbial agents contain Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans with a mass ratio of 5:3.5:1.5. The viable count of the compound microbial agents is 10 billion CFU / g.

[0132] Among them, the preparation method of the compound microbial agents includes the following steps: First, mix Bacillus subtilis, Bacillus licheniformis, and Bacillus coagulans in the compound microbial agents according to a mass ratio of 5:3.5:1.5 to obtain a microbial strain mixture, and then add zeolite powder and calcium carbonate and mix evenly to obtain the compound microbial agents; among them, based on the total mass of the compound microbial agents, the compound microbial agents include 50% of the microbial strain mixture, 25% of zeolite powder, and 25% of calcium carbonate.

[0133] Among them, the yeast metabolites are the same as those in Example 1.

[0134] (2) The preparation method of the yeast-derived bio-organic fertilizer suitable for aquaculture is the same as that of the yeast-derived bio-organic fertilizer suitable for aquaculture in Example 1.

[0135] Product indicators of the yeast-derived bio-organic fertilizer suitable for aquaculture: Total nutrients (sum of N, P 2 O 5 and K 2 O) (calculated based on the dry weight of the organic fertilizer) is 25%; moisture content is 0.48%; pH is 6.6; density is 1.28 g / cm 3 .

[0136] Application Example 1

[0137] Add the yeast-derived bio-organic fertilizer prepared in the example to water, and test its effects on dissolved oxygen in water, ammonia nitrogen removal rate, chlorophyll a content, and algal biomass and algal community structure.

[0138] The experimental pond was divided into eight regions of the same size, named as Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group, Example 7 group and control group. The Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Example 6 group and Example 7 group were respectively added with the bio-organic fertilizers prepared in Example 1, Example 2, Example 3, Example 4, Example 5, Example 6 and Example 7. The control group was not added with fertilizers. Except for the control group, the application rate of fertilizers was 4.0 kg / mu. The experimental conditions were outdoor natural light and temperature. Among them, the 0th day refers to the day after adding fertilizers. The weather conditions and water temperature during the experiment are recorded in Table 2 below.

[0139] Table 2 Weather Conditions and Water Temperature Record during the Experiment

[0140]

[0141] (1) Dissolved oxygen content in water

[0142] After adding fertilizers to each group of ponds, water samples 30 cm below the water surface were taken at the middle of the south bank of each group of ponds, 1 meter away from the pond bank, at the same time period (10 am) on the 0th, 1st, 2nd, 3rd, 4th, 5th and 6th days respectively, to analyze the dissolved oxygen content (DO) in the aquaculture water body of each group of ponds and evaluate the impact of different fertilizers on aquaculture water quality.

[0143] Among them, the dissolved oxygen content (DO) in water was detected by the LDO portable dissolved oxygen tester of HACH Company. The data of the dissolved oxygen content in water (unit: mg / L) obtained by detection are shown in Table 3 below. TM The data of the dissolved oxygen content in water are summarized in Table 3 below.

[0144] Table 3 Summary of Dissolved Oxygen Content Data in Water

[0145]

[0146] As can be seen from Table 4, on the 3rd day after adding the bio-organic fertilizers prepared in Examples 1-7, the dissolved oxygen content in water was significantly higher than that of the control group. Among them, the strains of the compound bacterial agents in the bio-organic fertilizers used in the Example 6 and Example 7 groups and the Example 1-5 groups were different. On the 1st day after adding the bio-organic fertilizers obtained in Example 6 and Example 7 respectively, the dissolved oxygen content in water and its increasing rate over time were lower than those of the Example 1-5 groups, indicating that the bio-organic fertilizers obtained by compounding preferred strains had more excellent effects on improving the dissolved oxygen content in water.

[0147] (2) Removal of ammonia nitrogen in water

[0148] After adding fertilizer treatment to each group of ponds, water samples 30 cm below the water surface were taken at the middle of the south bank of each pond, 1 meter away from the pond bank, at the same time period (10:00 am) on the 0th, 1st, 2nd, 3rd and 4th days respectively, and the main water physical and chemical factors of ammonia nitrogen (NH 2+ -N) in the aquaculture water bodies of each group of ponds were analyzed to evaluate the effects of different fertilizers on aquaculture water quality.

[0149] Ammonia nitrogen is a common harmful substance in aquaculture water bodies, mainly coming from the metabolic wastes of fish and the digestive metabolism process of feed. Higher concentrations will affect the physiological functions and enzyme activities of shrimp bodies, causing their metabolism to be unbalanced, growth to be inhibited, and disease resistance to decline. Ammonia nitrogen has two forms in the aqueous environment, NH 4+ and NH 3 , among which non-ionic ammonia (NH 3 ) causes serious harm to aquatic animals, corrodes the gill tissues of fish and shrimps, and destroys the respiratory function of gills.

[0150] The determination of the mass concentration (mg / L) of ammonia nitrogen (NH 2+ -N) in the water sample refers to the Nessler reagent spectrophotometric method for the determination of ammonia nitrogen in water quality specified in the National Environmental Protection Standard HJ535-2009 of the People's Republic of China. Then, the removal rate (α 1 ) of ammonia nitrogen in the experimental group was calculated based on the concentration of ammonia nitrogen in the detected water sample. The specific calculation formula is as follows:

[0151]

[0152] In the formula:

[0153] α 1 is the removal rate of ammonia nitrogen, and the value is expressed in %;

[0154] c 初始 is the mass concentration of ammonia nitrogen in each group of ponds at the initial stage (i.e., before adding fertilizer), with the unit of mg / L;

[0155] c n is the mass concentration of ammonia nitrogen in each group of ponds on the nth day after adding fertilizer, with the unit of mg / L, and n is the 1st, 2nd, 3rd and 4th days respectively;

[0156] △c = cK 初始 - cK n , that is, the difference between the mass concentration of ammonia nitrogen in the control group of ponds at the initial stage (before adding fertilizer in the experimental group and the competitor control group) and the mass concentration of ammonia nitrogen on the nth day (the nth day after adding fertilizer in the experimental group and the competitor control group), with the unit of mg / L.

[0157] The calculation formula for the removal rate (α 2 ) of ammonia nitrogen in the control group is as follows:

[0158]

[0159] In the formula:

[0160] α 2 is the removal rate of ammonia nitrogen, and the value is expressed in %;

[0161] cK 初始 is the mass concentration of ammonia nitrogen at the initial stage of the pond in the control group (before adding fertilizer to the experimental group and the competitor control group), and the unit is mg / L;

[0162] cK n is the mass concentration of ammonia nitrogen in the pond of the control group on the nth day after adding fertilizer, and the unit is mg / L. n is the 1st, 2nd, 3rd, and 4th days respectively.

[0163] The calculation data of the ammonia nitrogen removal rate of each group are shown in Table 4 below.

[0164] Table 4 Summary data of ammonia nitrogen removal rate

[0165]

[0166] As can be seen from Table 4, compared with the control group, the addition of fertilizers in Example Groups 1-7 significantly improved the ammonia nitrogen removal rate. Among them, after adding the biological organic fertilizers in Example Groups 1-5, the ammonia nitrogen removal rate gradually increased with the extension of time. Among them, the ammonia nitrogen removal rate in Example Groups 1-3 could reach more than 70% on the 4th day; while after adding the biological organic fertilizers in Example Groups 6-7, it showed a trend of first increasing and then decreasing with the extension of time, indicating that the biological organic fertilizers prepared in Example Groups 1-3 have more advantages in removing ammonia nitrogen in water.

[0167] (3) Chlorophyll a content

[0168] Chlorophyll is an indispensable substance in plant growth and an important basis for the survival and reproduction of aquatic animals. The chlorophyll content in water directly reflects the growth status of phytoplankton and algae in the water.

[0169] Samples were taken on the 0th, 1st, 2nd, 3rd, 4th, and 5th days during the experiment to measure the chlorophyll a content. The detection method of chlorophyll a content refers to 1 Materials and Methods in the reference (Chen Yuwei, Chen Kaining, Hu Yaohui. Discussion on the "hot ethanol method" for the determination of phytoplankton chlorophyll a and its measurement error [J]. Journal of Lake Sciences, 2006, (05): 550-552). The calculation data of the chlorophyll a content of each group are shown in Table 5 below.

[0170] Table 5 Summary of change data of chlorophyll a content

[0171]

[0172]

[0173] As can be seen from Table 5, compared with the control group, the addition of fertilizers in the fertilizer groups of Examples 1-7 inhibited the rate of decline of chlorophyll a, and starting from the 3rd day, the content of chlorophyll a was significantly increased. This indicates that the bio-organic fertilizer prepared in the examples is beneficial to increasing the content of chlorophyll a in the water body and has a promoting effect on the proliferation and growth of algae.

[0174] (4) Types of water body algae

[0175] On the 5th day of the experiment, 500 mL of water samples were taken from the ponds of each group. After sampling, Lugol's solution (take 20 g of iodine and 30 g of potassium iodide, add 500 mL of water, and dissolve to obtain Lugol's solution) was added and allowed to stand and precipitate for 24 h. The supernatant was removed with a siphon, and the sample was concentrated to 50 mL. A plankton counting frame was used to identify and count the algae, and the cell density of various algae was calculated. If the cell density of a certain type of algae accounts for 10% or more of the total cell density, then this type of algae is defined as the dominant species. The dominant species of algae in each group are shown in Table 6 below.

[0176] Table 6 Dominant species in each group during the experiment

[0177]

[0178] As can be seen from Table 6, compared with the control group, the addition of fertilizers in the fertilizer groups of Examples 1-4 increased the types of algae. This indicates that the bio-organic fertilizers prepared in Examples 1-4 can significantly promote the growth of algae in the aquaculture water body, increase the abundance of water body algae, and improve the dissolved oxygen conditions in the pond water body.

[0179] Although the specific implementation manners of the present invention are described above, it does not limit the protection scope of the present invention. Based on the technical solutions of the present invention, various modifications or deformations that can be made by those skilled in the art without creative labor are still within the protection scope of the present invention.

Claims

1. A yeast-derived bio-organic fertilizer suitable for aquaculture, characterized in that: Calculated by weight, it comprises: 40-80 parts of yeast metabolites, 15-30 parts of ammonium chloride, 10-20 parts of monoammonium phosphate and 3-8 parts of compound bacterial agent.

2. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1, characterized in that: In parts by weight, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40 to 70 parts of yeast metabolites; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40 to 50 parts of yeast metabolites; more preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 40 to 42 parts of yeast metabolites; And / or, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 15 to 25 parts of ammonium chloride; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 15 to 17 parts of ammonium chloride; And / or, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 10 to 15 parts of monoammonium phosphate; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 10 to 12 parts of monoammonium phosphate; And / or, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3 to 6 parts of a composite bacterial agent; preferably, the yeast-derived bio-organic fertilizer suitable for aquaculture comprises 3 to 5 parts of a composite bacterial agent.

3. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 1 or 2, characterized in that: The composite bacterial agent includes one or more than two selected from the group consisting of Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus laterosporus and Bacillus coagulans.

4. The yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 3, characterized in that: The weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens is 3-7:2-6:0.5-3; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus laterosporus is 3-7:2-6:0.5-3; or, the weight ratio of the Bacillus subtilis, Bacillus licheniformis and Bacillus coagulans is 3-7:2-6:0.5-3.

5. The yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1 to 4, characterized in that: The viable bacterial count of the composite bacterial agent is 9 to 11 billion cfu / g; and / or, the yeast-derived bio-organic fertilizer suitable for aquaculture has a total content of N, P2O5 and K2O of more than 10% on a dry basis; and / or, a pH value of 4.5 to 8.5; and / or, a density of 1.2 to 1.3 g / cm 3 .

6. A method for preparing a yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of claims 1 to 5, characterized in that: The following steps are involved: Yeast metabolites, ammonium chloride, monoammonium phosphate and a composite bacterial agent are mixed to obtain a yeast-derived bio-organic fertilizer suitable for aquaculture.

7. The method for preparing a yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 6, characterized in that: The yeast metabolites are metabolites produced by fermenting and extracting yeast using molasses or hydrolyzed sugar as a culture medium; And / or, based on the weight of the yeast metabolites, the yeast metabolites include 1% to 5% total nitrogen content, 11% to 16% potassium content, 2% to 8% sodium content, 34% to 40% organic matter content, 32% to 38% fulvic acid content, and / or 8% to 14% amino acid content.

8. A yeast-derived bio-organic fertilizer suitable for aquaculture, characterized in that: The yeast-derived bio-organic fertilizer suitable for aquaculture is prepared by the preparation method of the yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 6 or 7.

9. An application of a yeast-derived bio-organic fertilizer suitable for aquaculture in preparing fertilizer water, characterized in that: The yeast-derived bio-organic fertilizer suitable for aquaculture is the yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1 to 5 or the yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 8.

10. A use of a yeast-derived bio-organic fertilizer suitable for aquaculture in reducing the ammonia nitrogen content in water, increasing the chlorophyll content in water and / or increasing the types and contents of algae, characterized in that: The yeast-derived bio-organic fertilizer suitable for aquaculture is the yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1 to 5 or the yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 8.

11. A water fertilizer, characterized in that: The water fertilizer comprises the yeast-derived bio-organic fertilizer suitable for aquaculture as described in any one of claims 1 to 5 or the yeast-derived bio-organic fertilizer suitable for aquaculture as described in claim 8.

12. A composition for reducing the ammonia nitrogen content in water, increasing the chlorophyll content in water, and / or increasing the types and contents of algae, characterized in that: The composition comprises the yeast-derived bio-organic fertilizer suitable for aquaculture according to any one of claims 1 to 5 or the yeast-derived bio-organic fertilizer suitable for aquaculture according to claim 8.

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