Jinmetolachlor degrading bacterium DH5 and application thereof
By developing the Aspergillus fungus DH5, the problem of herbicide damage in the buckwheat field was solved, and the efficient degradation and drug damage relief of the Jinduer was achieved, and the environmentally friendly.
Patent Information
- Application Number
- CN202510232410.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2025-05-30
AI Technical Summary
The prior art lacks effective measures to degrade and prevent and control the drug damage caused by the herbicide Jinduer used in buckwheat fields.
A fungus of Aspergillus genus DH5 was developed. Through screening and identification, it was found that it could efficiently degrade Jindul. This strain is used to prepare herbicide Jinduer degrading bacteria agent, which is used to degrade Jinduer in the soil, thereby alleviating the drug damage to buckwheat.
The strain DH5 has a significant effect in degrading Jinduer, with a degradation rate of 40.43% in 7 days and a degradation rate of 46.53% in 21 days, effectively alleviating the drug damage of Jinduer to buckwheat, and has no pollution to humans, animals and the environment, and has good application prospects.
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Figure CN120059967A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of pendimethalin degradation, and more particularly to a pendimethalin-degrading bacterium DH5 and its application. Background Art
[0002] Weeds in buckwheat fields are the main factor affecting buckwheat yield. At present, agricultural and mechanical measures cannot be fully utilized for prevention and control, mainly relying on chemical herbicides. However, buckwheat is particularly sensitive to herbicides. When using herbicides for controlling gramineous weeds at the seedling stage, the dosage must be strictly controlled. For broad-leaved weeds in buckwheat fields, there are no available herbicides after emergence, and only individual herbicides in amide herbicides, such as pendimethalin (S - metolachlor), can be used for soil sealing treatment. However, when farmers use it, they often cannot accurately measure the size of the field, accurately weigh the dosage of the drug and the amount of water used, often resulting in phytotoxicity. There is no research on the prevention and control of herbicide phytotoxicity in buckwheat fields at home and abroad, and there is a lack of measures to solve the problems of buckwheat phytotoxicity and environmental hazards in the planting link.
[0003] The method of using microorganisms such as bacteria and fungi to alleviate herbicide phytotoxicity and degrade herbicides is safe and pollution-free, which is beneficial to improving crop quality, yield and safety. However, at present, strains for degrading the herbicide pendimethalin have not been reported.
[0004] In summary, how to provide a bacterium for degrading the herbicide pendimethalin to alleviate the phytotoxicity of pendimethalin to buckwheat is an urgent problem to be solved by those skilled in the art. Summary of the Invention
[0005] In view of this, the present invention provides a pendimethalin-degrading bacterium DH5 and its application.
[0006] The present invention takes soil samples from 0 - 10 cm from the surface of the plot where pendimethalin has been applied all year round. The bacterial cake of the strain is inverted on the basic inorganic salt solid medium with pendimethalin as the sole carbon source, and the microorganisms in the soil are preliminarily screened by observing whether a transparent circle can appear around the bacterial cake. The strain is rescreened by measuring the degradation rate through high performance liquid chromatography. The screened strain is identified by morphology and molecular biology to obtain a pendimethalin-degrading bacterium, a fungus of the genus Aspergillus (Aspergillus sp.). And the strain DH5 is subjected to pot control efficacy tests and field control efficacy tests on buckwheat seedlings.
[0007] In order to achieve the above object, the present invention adopts the following technical scheme:
[0008] A fungus of the genus Aspergillus DH5, named DH5, classified as Aspergillus sp., was deposited in the China Center for Type Culture Collection on July 1, 2024, with the deposit number CCTCC NO: M20241438, and the deposit address is Wuhan University, Wuhan, China.
[0009] Application of the above-mentioned Aspergillus sp. DH5 in degrading the herbicide S-metolachlor.
[0010] Application of the above-mentioned Aspergillus sp. DH5 in preparing a microbial agent for degrading the herbicide S-metolachlor.
[0011] Application of the above-mentioned Aspergillus sp. DH5 in increasing the yield and 1000-grain weight of buckwheat.
[0012] A microbial agent for degrading the herbicide S-metolachlor, comprising the above-mentioned Aspergillus sp. DH5.
[0013] A method for degrading the herbicide S-metolachlor in soil, by applying the above-mentioned Aspergillus sp. DH5.
[0014] As can be seen from the above technical solutions, compared with the prior art, the beneficial effects achieved by the present invention are as follows:
[0015] The S-metolachlor-degrading bacterium of the present invention is Aspergillus sp. DH5. The degradation rate of S-metolachlor in the culture medium by strain DH5 is 40.43% after 7 days of degradation, and 46.53% after 21 days of degradation. The Aspergillus sp. DH5 provided by the present invention is used for rapid degradation of S-metolachlor, alleviates the phytotoxicity of S-metolachlor in the seedling stage of buckwheat, is safe for humans and livestock, and has no pollution to the environment, and has good development and application prospects. Description of the Drawings
[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained according to the provided drawings without creative efforts.
[0017] Figure 1 It is the result diagram of the plate test in Example 2 of the present invention;
[0018] Figure 2 It is the morphological diagram of strain DH5 in Example 4 of the present invention;
[0019] Figure 3 It is the construction of the phylogenetic tree of strain DH5 in Example 4 of the present invention. Detailed Embodiments
[0020] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.
[0021] The reagents required for the present invention are conventional experimental reagents and are purchased from commercial channels; the experimental methods not mentioned are conventional experimental methods and will not be elaborated one by one here.
[0022] The components of the culture media used in the following examples are as follows:
[0023] LB solid medium: 10 g of peptone, 5 g of yeast extract powder, 10 g of NaCl, 15 g of agar, 1000 mL of distilled water.
[0024] LB liquid medium: 10 g of peptone, 5 g of yeast extract powder, 10 g of NaCl, 1000 mL of distilled water.
[0025] PDA medium: 200 g of peeled potatoes, 18 g of agar, 20 g of glucose, 1000 mL of distilled water.
[0026] Basic inorganic salt medium: KH 2 PO 4 1.6 g, K 2 HPO 4 0.52 g, NH 4 NO 3 1.0 g, 0.5 g of NaCl, MgSO 4 ·7H 2 O 0.5 g, 1000 mL of distilled water.
[0027] Carbon source determination basic medium: KH 2 PO 4 2.38 g, K 2 HPO 4 ·3H 2 O 5.65 g, (NH 4 ) 2 SO 4 2.64 g, MgSO 4 ·7H 2 O 1 g, CuSO 4 ·5H 2 O 6.4 mg, ZnSO 4 ·7H 2 O 1.5 mg, FeSO 4 ·7H 2 O 1.1 mg, MnCl2 ·7H 2 O 7.9 mg, agar 15 g, distilled water 1000 mL.
[0028] Nitrogen source determination basal medium: glucose 10 g, K 2 HPO 4 ·3H 2 O 1 g, MgSO 4 ·7H 2 O 5 g, NaCl 5 g, FeSO 4 ·7H 2 O 10 mg, agar 15 g, distilled water 1000 mL.
[0029] Malonate test medium: sodium malonate 3 g, yeast extract 1 g, NaCl 2 g, (NH 4 ) 2 SO 4 2 g, KH 2 PO 4 0.4 g, K 2 HPO 4 ·3H 2 O 0.6 g, bromothymol blue 25 mg, distilled water 1000 mL, pH 7.4.
[0030] Methyl red test medium: peptone 7.0 g, glucose 5 g, NaCl 5 g, distilled water 1000 mL.
[0031] Starch hydrolysis test medium: soluble starch 2 g, beef extract 3 g, peptone 5 g, glucose 2.5 g, agar 18 g, distilled water 1000 mL, pH 7.0.
[0032] Fat hydrolysis test medium: peptone 10 g, CaCl 2 ·2H 2 O 0.1 g, agar 17 g, distilled water 1000 mL, pH 7.4.
[0033] NA medium: beef extract 3 g, peptone 5 g, glucose 2.5 g, agar 18 g, distilled water 1000 mL, pH 7.0.
[0034] Example 1
[0035] Isolation of strains
[0036] Soil samples were taken from the farmland plot of the new campus of Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, at a depth of 0 - 10 cm from the ground surface. 5 g of soil samples were weighed and placed in 95 mL of liquid basic inorganic salt culture medium, and S-metolachlor was added as the sole carbon source for microbial growth and utilization, so that the final concentration of S-metolachlor was 50 mg / L. The mixture was cultured on a shaker at 150 r / min and 28 °C for 7 days with shaking. Then, 1 mL was transferred to the basic inorganic salt culture medium containing 100 mg / L of S-metolachlor and cultured for 7 days. Subsequently, 1 mL was transferred to the basic inorganic salt culture medium containing 150 mg / L of S-metolachlor and cultured for 7 days. By gradually increasing the concentration of S-metolachlor, the selective pressure of the herbicide on microorganisms was increased, aiming to isolate and screen highly efficient herbicide-degrading strains. After 21 days of enrichment culture, the basic inorganic salt culture medium was made into 10 -1 、10 -3 、10 -4 、10 -5 different concentrations. 200 μL of the culture medium with different concentrations was respectively spread on PDA and LB media. 10 -3 concentration of the culture medium was spread on PDA, and 10 -4 and 10 -5 concentrations of the culture medium were respectively spread on LB for the isolation of fungi and bacteria. This was repeated 3 times. After inoculation, it was placed in an incubator at 25 °C for 2 - 5 days. Different morphological single colonies were picked for purification, numbered, preserved, and reserved for use.
[0037] Example 2
[0038] Primary screening of S-metolachlor-degrading bacteria by plate method
[0039] The isolated bacterial strains were secondarily purified on LB medium by the streak plate method, and the fungal strains were secondarily purified on PDA medium by the single spore isolation method. The purified bacterial strains were spread on LB medium by the dilution plating method and cultured for 3 days, and the fungal strains were inoculated on PDA medium and cultured for 5 days. 500 μL of the herbicide S-metolachlor was quickly added to 99.5 mL of the basic inorganic salt medium at a temperature of 40 ± 2.5 °C to make the total volume of the solution in the medium 100 mL, and immediately mixed evenly. Before the medium solidified, it was poured into glass petri dishes, 20 mL per dish. After the medium solidified in the petri dishes, the bacterial and fungal colonies with a diameter of 5 mm were inverted and inoculated into the petri dishes. The non-inoculated petri dishes were used as controls. Each treatment was repeated 3 times and cultured at 25 °C. Whether there was a clear zone around the colonies was observed. After 7 days of culture, the diameter of the clear zone was measured to preliminarily determine whether the strains could utilize the herbicide S-metolachlor. The results are shown in Table 1, Figure 1 as follows.
[0040] Table 1 Results of plate tests
[0041]
[0042] After primary screening, one strain was obtained and named DH5. Strain DH5 showed a clear zone on the plate. The diameter D of the clear zone was 1.06 cm, indicating its ability to utilize the herbicide S-metolachlor.
[0043] Example 3
[0044] Determination of the degradation rate of S-metolachlor by strain DH5
[0045] Method for determining the degradation rate of S-metolachlor: Take 5 mL of the fungal suspension to be tested with a spore concentration of 1×10 7 CFU / mL, transfer it aseptically into 95 mL of a basal inorganic salt medium with a S-metolachlor concentration of 100 mg / L, and incubate it at a constant temperature in a shaker at 25 °C and 180 r / min for 7 d and 21 d to obtain the test solution. Take 5 mL of the test solution and place it in a 50 mL centrifuge tube, add 5 mL of acetonitrile, shake it on a shaker at 25 °C and 220 r / min for 1 h, add 1 g of NaCl, and shake until obvious liquid stratification can be observed. Then centrifuge it at 4000 g for 5 min, pipette the upper organic liquid, filter the supernatant through a 0.22 μm disposable filter, and determine the degradation rate using a high-performance liquid chromatograph.
[0046] Instrument conditions: The concentration of S-isopropyl metolachlor in the sample was determined by liquid chromatography. The mobile phase was acetonitrile: ultrapure water (20:80, V / V), the detection wavelength of S-isopropyl metolachlor was 254 nm, the flow rate was 1.0 mL / min, the injection volume was 5 μL, the column temperature was 25 °C, and the retention time was 8 min. The substance was determined according to its elution time, and the content of S-isopropyl metolachlor was quantitatively calculated according to its peak area.
[0047]
[0048] The results are shown in Table 2.
[0049] Table 2 Degradation rate results of the isolated strains on S-isopropyl metolachlor
[0050]
[0051] Example 4
[0052] Identification of strain DH5
[0053] (1) Morphological identification
[0054] The strain was spread on PDA medium by the single spore isolation method. By observing the morphology, it was found that strain DH5 was a fungus with white or light-colored mycelia that turned grayish-brown when old, and the sporangia were spherical and irregular ( Figure 2 ).
[0055] (2) Molecular biological identification
[0056] The DNA was extracted according to the instructions of the Ezup Fungal Genomic DNA Extraction Kit (centrifugal column type), purchased from Sangon Biotech (Shanghai) Co., Ltd. The PCR primers for strain DH5 were ITS1 and ITS4 respectively, and the primer sequences are shown in Table 3 for details.
[0057] The 25 μL reaction system contained: 17 μL of ddH 2 O, 2.5 μL of 10×PCR Buffer, 2 μL of dNTP, 1 μL of upstream primer, 1 μL of downstream primer, 0.5 μL of Taq DNA polymerase, and 1 μL of template DNA. PCR reaction conditions: pre-denaturation at 94°C for 5 min; denaturation at 94°C for 30 s; annealing temperature for 30 s; extension at 72°C for 1 min; 34 cycles; extension at 72°C for 10 min.
[0058] The PCR products were detected by 1% agarose gel electrophoresis and sent to Sangon Biotech (Shanghai) Co., Ltd. for 16S rDNA sequencing, and the sequencing results are as follows. The obtained 1 gene sequence was compared with the GenBank nucleic acid database, and the strain sequences with higher similarity were selected for analysis, and the phylogenetic tree was constructed using the software MEGA7.0. Strain DH5 clustered with Aspergillus sp. (accession number: OY740230) ( Figure 3 ).
[0059] Table 3 Primers and Sequences
[0060]
[0061] ITS1 ITS4:
[0062]
[0063] Based on the comprehensive morphological and molecular biological identification results, the strain DH5 was identified as a fungus of the genus Aspergillus (Aspergillus sp.).
[0064] (3) Preservation
[0065] The Aspergillus fungus was named DH5, and its taxonomic name is Aspergillus sp. It was preserved in the China Center for Type Culture Collection on July 1, 2024, with the preservation number CCTCC NO: M 20241438, and the preservation address is Wuhan University, Wuhan, China.
[0066] Example 5
[0067] Mitigation effect of strain DH5 on the phytotoxicity of Pendimethalin in pot experiments
[0068] The seeds of buckwheat were dressed with strain DH5, and then herbicides with different concentrations were sprayed. The degradation effect of strain DH5 on Pendimethalin and the mitigation effect on phytotoxicity were analyzed through the change of phytotoxicity index.
[0069] The strain DH5 was cultured in PDB medium in a shaker at 25 °C and 180 r / min for 3 days, and then diluted to a spore concentration of 1×10 7 CFU / mL to prepare a spore suspension; for seed dressing of buckwheat, 20 mL of the spore suspension was added to every 20 g of seeds, stirred evenly, and placed in a cool and dry place to dry for later use.
[0070] There were two pot experiments in total: one was conducted with sterilized soil (July 2023) and the other with non-sterilized soil (December 2023).
[0071] For the first pot experiment, soil samples were taken from the farm of Inner Mongolia Agricultural University, sterilized at 180 °C for 2 h in an oven, and then mixed evenly with vermiculite at a volume ratio of 2:1 for use; for the second pot experiment, the soil from the new area farm of Inner Mongolia Agricultural University was used directly without sterilization, and the rest was the same as the first pot experiment.
[0072] For each treatment, 6 herbicide concentrations of 0, 80, 100, 120, 140, and 160 mL / 667 m 2 were set, with 3 replicates.
[0073] After sowing, the soil was covered with 2 - 3 cm of soil. The soil surface area was calculated based on the diameter of the soil in different flower pots, and the water consumption was 60 kg per 667 m 2 The herbicide dosages were 0, 80, 100, 120, 140, and 160 mL / 667 m 2Six different application concentrations were used. The water consumption and chemical application amount in the flower pot were calculated according to the soil surface area in the flower pot, and then the herbicide Pendimethalin was sprayed randomly. Ten days after sowing, the phytotoxicity level was investigated according to the phytotoxicity grading standard (Table 4), and the phytotoxicity index and the ratio of reducing phytotoxicity were calculated.
[0074] Table 4 Phytotoxicity grading standard of Pendimethalin on buckwheat
[0075]
[0076] Note: During the investigation, record the number of damaged buckwheat seedlings and the damage level, and calculate the phytotoxicity index and phytotoxicity control effect.
[0077]
[0078]
[0079] The results are shown in Tables 5, 6, 7, and 8.
[0080] Table 5 Phytotoxicity index of buckwheat after seed dressing with strains and spraying Pendimethalin (July 2023, soil sterilized)
[0081]
[0082] Table 6 Ratio of reducing phytotoxicity of buckwheat after seed dressing with strains to spraying Pendimethalin (%) (July 2023, soil sterilized)
[0083]
[0084] Table 7 Phytotoxicity index of buckwheat after seed dressing with strains and spraying Pendimethalin (December 2023, soil not sterilized)
[0085]
[0086] Table 8 Ratio of reducing phytotoxicity of buckwheat after seed dressing with strains to spraying Pendimethalin (%) (December 2023, soil not sterilized)
[0087]
[0088] As can be seen from Tables 5 and 6, under the condition of soil sterilization, when applying the herbicide at 80, 100, 120, 140, 160 mL / 667 m 2 , the strain DH5 could alleviate the phytotoxicity of Pendimethalin to buckwheat. Among them, when the herbicide dosage was 100 mL / 667 m 2 , the effect was the best, and the reduction ratio was 89.1%. Followed by 80 mL / 667 m 2 and 140 mL / 667 m 2, the reduction ratios were 74.5% and 48.3% respectively. As can be seen from Table 7 and Table 8, when applying herbicides at 80, 100, 120, 140, 160 mL / 667m 2 in non-sterilized soil, strain DH5 could alleviate the phytotoxicity of pendimethalin to buckwheat. Among them, when the herbicide dosage was 80 mL / 667m 2 , the effect was the best, and the reduction ratio was 84.0%. Followed by 100 mL / 667m 2 , and the reduction ratios were 54.7% respectively.
[0089] Example 6
[0090] Alleviating effect of strain DH5 on pendimethalin phytotoxicity in field trials
[0091] Buckwheat seeds were dressed with strain DH5, and then different concentrations of herbicides were sprayed. The degradation effect of strain DH5 on pendimethalin and the alleviating effect on phytotoxicity were analyzed through the change of phytotoxicity index.
[0092] The test site was set at the teaching base of Inner Mongolia Agricultural University in Hailiutu, Tumote Left Banner, Hohhot, Inner Mongolia. In the test, 6 concentrations of pendimethalin herbicide, namely 0, 80, 100, 120, 140, 160 mL / 667m 2 , were set for each treatment, and 3 replicates were set. The pretreatment method of dressing seeds with spore suspension was the same as that in Example 5.
[0093] Sowing was carried out on July 20, 2023, with a row spacing of 40 cm. A furrow with a depth of 4 - 5 cm was opened with a furrow opener. The seeds dressed with spore suspension were sown and immediately covered with soil, and then different concentrations of soil-sealing herbicide pendimethalin were applied immediately. The herbicide was diluted according to 60 kg of water consumption per 667m 2 , and 6 different application concentrations of herbicide were 0, 80, 100, 120, 140, 160 mL / 667m 2 . 15 days after sowing, the phytotoxicity level was investigated according to the phytotoxicity grading standard (Table 4), and the phytotoxicity index and the ratio of alleviating phytotoxicity were calculated. The calculation formulas were the same as those in Example 5.
[0094] The effect of strain DH5 on alleviating buckwheat seedling phytotoxicity can be seen in Table 9 and Table 10.
[0095] Table 9 Phytotoxicity index of buckwheat after spraying pendimethalin with strain dressing in field trials
[0096]
[0097] Table 10 Alleviating effect of strain dressing on buckwheat phytotoxicity after spraying pendimethalin in field trials (%)
[0098]
[0099] As can be seen from Table 9 and Table 10, in the field experiment, when applying herbicides at 80, 100, 120, 140, and 160 mL / 667m 2 , the strain DH5 could relieve the phytotoxicity of pendimethalin to buckwheat. Among them, when the herbicide dosage was 80, 100, and 140 mL / 667m 2 , the effects were the best, and the reduction ratios were 38.9%, 34.3%, and 35.3% respectively. Followed by 160 mL / 667m 2 , and the reduction ratio was 27.0%.
[0100] Example 7
[0101] Effect of strain DH5 on the yield and 1000-grain weight of buckwheat in field experiment
[0102] Buckwheat seeds were dressed with strain DH5, and then different concentrations of herbicides were sprayed. The yield-increasing effect of the isolated strain was analyzed through yield and 1000-grain weight.
[0103] The test site was set at the teaching base of Inner Mongolia Agricultural University in Hailiutu, Tumd Left Banner, Hohhot, Inner Mongolia. In the test, each treatment had 6 pendimethalin concentrations of 0, 80, 100, 120, 140, 160 mL / 667m 2 and an artificial weeding control, with 3 replicates. The pretreatment method of dressing seeds with spore suspension was the same as that in Example 5.
[0104] Sowing was carried out on July 20, 2023. The row spacing was set at 40 cm. A furrow with a depth of 4 - 5 cm was opened with a furrow opener. The seeds dressed with spore suspension were sown and immediately covered with soil, and then different concentrations of soil-sealing herbicide pendimethalin were immediately applied. The herbicide was diluted according to 60 kg of water consumption per 667m 2 and 6 different application concentrations of 0, 80, 100, 120, 140, 160 mL / 667m 2 . After harvesting, the yield and 1000-grain weight were measured. The yield-increasing effect of strain DH5 is shown in Table 11 and Table 12.
[0105] Table 11 Effect of strain dressing on the yield of buckwheat after spraying pendimethalin in field experiment (kg / 667m 2 )
[0106]
[0107] Table 12 Effect of strain dressing on the 1000-grain weight of buckwheat after spraying pendimethalin in field experiment (g)
[0108]
[0109] As can be seen from Table 11, when the pendimethalin dosage was 100 mL / 667m without applying the microbial agent CK 2At this time, there was no significant difference in yield compared with the manual weeding control, and it was greater than 100 mL / 667 m 2 The yield decreased significantly. When the microbial agent DH5 was applied, when the dosage of the herbicide S-metolachlor was greater than 100 mL / 667 m 2 At this time, compared with other herbicide concentrations, the yield decreased significantly, indicating that the ability of the microbial agent DH5 to alleviate the yield reduction caused by the herbicide decreased when the dosage of the herbicide S-metolachlor was greater than 100 mL / 667 m 2 As can be seen from Table 12, when no microbial agent (CK) was used and the dosage of the herbicide S-metolachlor was 80 mL / 667 m 2 At this time, there was no significant difference in 1000-grain weight compared with the manual weeding control, indicating that when the dosage of S-metolachlor was 80 mL / 667 m 2 It was safe for buckwheat and did not reduce the 1000-grain weight. When the dosage of S-metolachlor was greater than 80 mL / 667 m 2 , the 1000-grain weight was significantly lower than that of the manual weeding control, indicating that a herbicide dosage greater than 80 mL / 667 m 2 would reduce the 1000-grain weight of buckwheat. When the microbial agent DH5 was applied and the dosage of the herbicide S-metolachlor was 80 mL / 667 m 2 At this time, there was no significant difference in 1000-grain weight compared with the manual weeding control. When the dosage of S-metolachlor was greater than 80 mL / 667 m 2 , the 1000-grain weight was significantly lower than that of the manual weeding control.
[0110] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the embodiments, reference can be made to each other.
[0111] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. An Aspergillus fungus DH5, characterized in that The Aspergillus fungus is named DH5, and its classification name is Aspergillus sp. It was deposited in the China Center for Type Culture Collection on July 1, 2024, with the deposit number CCTCCNO: M 20241438, and the deposit address is Wuhan University, Wuhan, China.
2. Use of the Aspergillus fungus DH5 according to claim 1 in degrading the herbicide Jindul.
3. Use of the Aspergillus fungus DH5 described in claim 1 in preparing a herbicide Jindul-degrading bacterial agent.
4. Use of the Aspergillus fungus DH5 according to claim 1 in increasing buckwheat yield and thousand-grain weight.
5. A herbicide Jindul degrading bacterial agent, characterized in that: It comprises the Aspergillus fungus DH5 described in claim 1.
6. A method for degrading the herbicide Jindul in soil, characterized in that: The Aspergillus fungus DH5 according to claim 1 is used.