Bacillus thuringiensis and application thereof

Abstract

The invention discloses a bacillus thuringiensis strain and an application thereof. Belongs to the technical field of gold metolachlor degradation. The method comprises the following steps: taking a soil sample from a perennial land where Jinmetor is applied, taking a bacterial cake of the strain in a basic inorganic salt solid culture medium which takes Jinmetor as a unique carbon source, carrying out primary screening on microorganisms in the soil by observing whether a transparent circle can appear around the bacterial cake or not, determining the degradation rate of the strain through a liquid chromatography, and carrying out secondary screening, the screened strain is identified through morphology, physiology, biochemistry and molecular biology, and the bacillus thuringiensis JGZ26 is obtained. When the bacillus thuringiensis JGZ26 is used for potting control effect tests and field control effect tests on buckwheat seedlings, the phytotoxicity in the buckwheat seedling stage can be relieved, and the bacillus thuringiensis JGZ26 is safe to people and livestock.

Description

Technical Field

[0001] The present invention relates to the technical field of pendimethalin degradation, and more specifically, to a Bacillus thuringiensis strain and its application. Background Art

[0002] Weeds in buckwheat fields are the main factors affecting buckwheat yield. At present, agricultural and mechanical measures cannot be fully utilized for prevention and control, and mainly rely on chemical herbicides. However, buckwheat is particularly sensitive to herbicides. When using herbicides to control grassy weeds at the seedling stage, the dosage must be strictly controlled. For broad-leaved weeds in buckwheat fields, there are no available herbicides after emergence, and only individual herbicides in amide herbicides, such as pendimethalin (S - metolachlor), can be used for soil sealing treatment. However, when farmers use it, they often cannot accurately measure the size of the field, accurately weigh the dosage of the drug and the amount of water used, which often leads to phytotoxicity. There is no research on the prevention and control of herbicide phytotoxicity in buckwheat fields at home and abroad, and there is a lack of measures to solve the problems of buckwheat phytotoxicity and environmental hazards in the planting process.

[0003] The method of using microorganisms such as bacteria and fungi to alleviate herbicide phytotoxicity and degrade herbicides is safe and pollution - free, and is beneficial to improving crop quality, yield and safety. However, at present, strains capable of degrading the herbicide pendimethalin have not been reported.

[0004] In summary, how to provide a degradation bacterium of the herbicide pendimethalin to alleviate the phytotoxicity of pendimethalin to buckwheat is an urgent problem to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a Bacillus thuringiensis strain and its application.

[0006] In order to achieve the above - mentioned purpose, the present invention adopts the following technical solutions:

[0007] A Bacillus thuringiensis strain JGZ26, named JGZ26, its taxonomic name is Bacillus thuringiensis, was deposited in the China Center for Type Culture Collection on May 22, 2023, with the deposit number CCTCC NO: M 2023806, and the deposit address is Wuhan University, Wuhan, China.

[0008] The above - mentioned Bacillus thuringiensis strain JGZ26 is used in the degradation of the herbicide pendimethalin.

[0009] The above - mentioned Bacillus thuringiensis strain JGZ26 is used in alleviating the phytotoxicity of pendimethalin to plants.

[0010] The above - mentioned Bacillus thuringiensis strain JGZ26 is used in improving the yield and 1000 - grain weight of plants.

[0011] Further, the plant is buckwheat.

[0012] An acetochlor-degrading microbial agent, which includes the above-mentioned Bacillus thuringiensis JGZ26.

[0013] As can be seen from the above technical solutions, compared with the prior art, the beneficial effects achieved by the present invention are as follows:

[0014] The acetochlor-degrading bacterium of the present invention is Bacillus thuringiensis JGZ26. Bacillus thuringiensis JGZ26 degrades acetochlor in the culture medium for 7 days, and the degradation rate is 56.32%. It degrades acetochlor in the culture medium for 21 days, and the degradation rate is 71.61%. The Bacillus thuringiensis JGZ26 provided by the present invention is used for the rapid degradation of acetochlor, alleviates the phytotoxicity of buckwheat seedlings at the seedling stage, is safe for humans and livestock, and has no pollution to the environment, and has good development and application prospects. Description of the Drawings

[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained according to the provided drawings without creative efforts.

[0016] Figure 1 It is the result diagram of the plate test in Embodiment 2 of the present invention;

[0017] Figure 2 It is the strain morphology diagram of strain JGZ26 in Embodiment 4 of the present invention;

[0018] Figure 3 It is the Gram staining diagram of strain JGZ26 in Embodiment 4 of the present invention;

[0019] Figure 4 It is the phylogenetic tree of strain JGZ26 in Embodiment 4 of the present invention. Detailed Embodiments

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0021] The agents required for the present invention are conventional experimental agents, which are purchased from commercial channels; the experimental methods not mentioned are conventional experimental methods, and will not be elaborated here one by one.

[0022] The components of the culture media used in the following examples are as follows:

[0023] LB solid medium: 10 g of peptone, 5 g of yeast extract powder, 10 g of NaCl, 15 g of agar, 1000 mL of distilled water.

[0024] LB liquid medium: 10 g of peptone, 5 g of yeast extract powder, 10 g of NaCl, 1000 mL of distilled water.

[0025] PDA medium: 200 g of peeled potatoes, 18 g of agar, 20 g of glucose, 1000 mL of distilled water.

[0026] Basic inorganic salt medium: KH 2 PO 4 1.6 g, K 2 HPO 4 0.52 g, NH 4 NO 3 1.0 g, 0.5 g of NaCl, MgSO 4 ·7H 2 O 0.5 g, 1000 mL of distilled water.

[0027] Basic medium for carbon source determination: KH 2 PO 4 2.38 g, K 2 HPO 4 ·3H 2 O 5.65 g, (NH 4 ) 2 SO 4 2.64 g, MgSO 4 ·7H 2 O 1 g, CuSO 4 ·5H 2 O 6.4 mg, ZnSO 4 ·7H 2 O 1.5 mg, FeSO 4 ·7H 2 O 1.1 mg, MnCl 2 ·7H 2 O 7.9 mg, 15 g of agar, 1000 mL of distilled water.

[0028] Basic medium for nitrogen source determination: 10 g of glucose, K 2 HPO 4 ·3H 2 O 1 g, MgSO 4 ·7H 2 O 5 g, 5 g of NaCl, FeSO 4·7H 2 O 10 mg, agar 15 g, distilled water 1000 mL.

[0029] Malonate test medium: Sodium malonate 3 g, yeast extract 1 g, NaCl 2 g, (NH 4 ) 2 SO 4 2 g, KH 2 PO 4 0.4 g, K 2 HPO 4 ·3H 2 O 0.6 g, bromothymol blue 25 mg, distilled water 1000 mL, pH 7.4.

[0030] Methyl red test medium: Peptone 7.0 g, glucose 5 g, NaCl 5 g, distilled water 1000 mL.

[0031] Starch hydrolysis test medium: Soluble starch 2 g, beef extract 3 g, peptone 5 g, glucose 2.5 g, agar 18 g, distilled water 1000 mL, pH 7.0.

[0032] Lipid hydrolysis test medium: Peptone 10 g, CaCl 2 ·2H 2 O 0.1 g, agar 17 g, distilled water 1000 mL, pH 7.4.

[0033] NA medium: Beef extract 3 g, peptone 5 g, glucose 2.5 g, agar 18 g, distilled water 1000 mL, pH 7.0.

[0034] Example 1

[0035] Isolation of strains

[0036] Soil samples were taken from the 0 - 10 cm depth of the soil in Ningcheng County, Chifeng City, Inner Mongolia. Weigh 5 g of soil sample and place it in a liquid basal inorganic salt culture medium containing 95 mL. Add pendimethalin as the sole carbon source for microbial growth and utilization, so that the final concentration of pendimethalin is 50 mg / L. Incubate it on a shaker at 150 r / min and 28 °C for 7 days with enrichment culture. Take 1 mL and transfer it to a basal inorganic salt culture medium containing 100 mg / L pendimethalin, and culture for 7 days. Then take 1 mL and transfer it to a basal inorganic salt culture medium containing 150 mg / L pendimethalin and culture for 7 days. By gradually increasing the concentration of pendimethalin, the selection pressure of the herbicide on microorganisms is increased, aiming to isolate and screen highly efficient herbicide-degrading strains. After 21 days of enrichment culture, the basal inorganic salt culture medium was made into 10 -1 、10 -3 、10 -4 、10 -5At different concentrations, 200 μL of culture media at different concentrations were respectively applied to PDA and LB media. 10 -3 concentration of the culture medium was applied to PDA, and 10 -4 and 10 -5 concentration of the culture medium were applied to LB for the isolation of fungi and bacteria. It was repeated 3 times. After inoculation, it was placed in an incubator at 25 °C for 2 - 5 days. Single colonies with different morphologies were picked for purification, numbered, preserved, and reserved for use.

[0037] Example 2

[0038] Primary screening of pendimethalin - degrading bacteria by plate method

[0039] The isolated bacterial strains were secondarily purified on LB medium by the streak plate method, and the fungal strains were secondarily purified on PDA medium by the single - spore isolation method. The purified bacterial strains were applied to LB medium by the dilution - plating method and cultured for 3 days, and the fungal strains were inoculated into PDA medium and cultured for 5 days. 500 μL of the herbicide pendimethalin was quickly added to 99.5 mL of the basal inorganic salt medium at a temperature of 40 ± 2.5 °C to make the total volume of the solution in the medium 100 mL, and it was immediately mixed evenly. Before the medium solidified, it was poured into glass Petri dishes, 20 mL per dish. After the medium solidified in the Petri dishes, bacterial and fungal discs with a diameter of 5 mm were inverted and inoculated into the Petri dishes. The non - inoculated Petri dishes were used as controls. Each treatment was repeated 3 times and cultured at 25 °C. Observe whether there is a clear zone around the discs. After culturing for 7 days, measure the diameter of the clear zone to preliminarily judge whether the strain can utilize the herbicide pendimethalin. The results are shown in Table 1 Figure 1 as follows.

[0040] Table 1 Results of plate tests

[0041]

[0042] After primary screening, 1 strain was obtained and named JGZ26. Strain JGZ26 showed a clear zone on the plate, and the diameter D of the clear zone was 1.52 cm, indicating its ability to utilize the herbicide pendimethalin.

[0043] Example 3

[0044] Determination of the pendimethalin degradation rate of strain JGZ26

[0045] Method for determining the pendimethalin degradation rate: Take a concentration of 1×10 85 mL of the bacterial suspension to be tested at CFU / mL was aseptically transferred into 95 mL of a basal inorganic salt medium with a pendimethalin concentration of 100 mg / L, and incubated at a constant temperature in a shaker at 25 °C and 180 r / min for 7 d and 21 d to obtain the test solution. 5 mL of the test solution was placed in a 50 mL centrifuge tube, 5 mL of acetonitrile was added, and the mixture was shaken on a shaker at 25 °C and 220 r / min for 1 h. 1 g of NaCl was added and shaken until obvious liquid layering was observed. Then, it was centrifuged at 4000 g for 5 min, and the upper organic liquid was aspirated with a pipette. The supernatant was filtered through a 0.22 μm disposable filter, and the degradation rate was determined using a high performance liquid chromatograph after filtration.

[0046] Instrument conditions: The concentration of S - metolachlor in the sample was determined by liquid chromatography. The mobile phase was acetonitrile: ultrapure water (20:80, V / V). The detection wavelength of S - metolachlor was 254 nm, the flow rate was 1.0 mL / min, the injection volume was 5 μL, the column temperature was 25 °C, and the retention time was 8 min. The substance was identified according to its peak time, and the content of S - metolachlor was quantitatively calculated according to its peak area.

[0047]

[0048] The results are shown in Table 2.

[0049] Table 2 Degradation rate results of the isolated strain on S - metolachlor

[0050]

[0051]

[0052] Example 4

[0053] Identification of strain JGZ26

[0054] (1) Morphological identification

[0055] The strain was spread on an LB solid medium by the dilution method to grow single colonies. Strain JGZ26 was light yellow, with a neat edge, opaque, slightly raised, and waxy ( Figure 2 ).

[0056] (2) Physiological and biochemical identification

[0057] The test referred to the "Manual for Systematic Identification of Common Bacteria" to determine the physiological and biochemical indexes of the strain, such as the utilization of carbon sources, nitrogen sources, malonate, methyl red test, starch hydrolysis, oxidase test, and catalase test. Strain JGZ26 was a Gram - positive bacterium ( Figure 3) It can utilize malonate, shows a positive catalase reaction, and cannot produce oxidase. Strain JGZ26 can decompose starch and fat, can utilize glucose, maltose, rhamnose, and mannitol as the sole carbon source, and potassium nitrate, sodium nitrate, and histidine can be used as the sole nitrogen source. See Table 3 for details.

[0058] Table 3 Physiological and Biochemical Characteristics of Strain JGZ26

[0059]

[0060] Note: “+” indicates positive, and “-” indicates negative.

[0061] (3) Molecular Biology Identification

[0062] DNA extraction was carried out according to the instructions of the TIAN GEN Bacterial Genomic DNA Extraction Kit (centrifugal column type) (Beijing, China), purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd. The PCR primers for strain JGZ26 were 27F and 1492R, 7F and 1540R, rpoBR and rpoBF, trpBF and trpBR respectively. See Table 4 for primer sequences.

[0063] The 25 μL reaction system contained: 17 μL of ddH 2 O, 2.5 μL of 10×PCR Buffer, 2 μL of dNTP, 1 μL of upstream primer, 1 μL of downstream primer, 0.5 μL of Taq DNA polymerase, and 1 μL of template DNA. PCR reaction conditions: pre-denaturation at 94 °C for 5 min; denaturation at 94 °C for 30 s; annealing temperature for 30 s; extension at 72 °C for 1 min; 34 cycles; extension at 72 °C for 10 min.

[0064] The PCR products of the 4 genes were detected by 1% agarose gel electrophoresis and sent to Shanghai Sangon Biotech Co., Ltd. for 16S rDNA sequencing. The sequencing results are as follows. The 4 gene sequences obtained were spliced, and the splicing results were compared with the Gen Bank nucleic acid database. The strain sequences with higher similarity were selected for analysis, and the phylogenetic tree was constructed using the software MEGA7.0. Strain JGZ26 and Bacillus thuringiensis (MG011551.1) clustered together ( Figure 4 ).

[0065] Table 4 Primers and Sequences

[0066]

[0067] 27F 1492R:

[0068]

[0069] 7F 1540R:

[0070]

[0071] rpoBF rpoBR:

[0072]

[0073] trpBF trpBR:

[0074]

[0075] Based on the comprehensive identification results of morphology, physiology, biochemistry and molecular biology, the strain JGZ26 was identified as Bacillus thuringiensis.

[0076] (4) Preservation

[0077] The above-mentioned Bacillus thuringiensis was named JGZ26, and its taxonomic name is Bacillus thuringiensis. It was preserved in the China Center for Type Culture Collection on May 22, 2023, with the preservation number CCTCC NO: M 2023806, and the preservation address is Wuhan University, Wuhan, China.

[0078] Example 5

[0079] Mitigation effect of strain JGZ26 on pendimethalin phytotoxicity in pot experiments

[0080] The seeds of buckwheat were dressed with strain JGZ26, and then different concentrations of herbicides were sprayed. The degradation effect of strain JGZ26 on pendimethalin and the mitigation effect of phytotoxicity were analyzed through the change of phytotoxicity index.

[0081] The strain JGZ26 was cultured in LB liquid medium in a shaker at 25 °C and 180 r / min, and diluted to 1×10 8 CFU / mL to prepare a microbial agent; for buckwheat seed dressing, 20 mL of the microbial agent was added to every 20 g of seeds and stirred evenly, and then placed in a cool and dry place to dry for later use.

[0082] There were two pot experiments in total: experiments were carried out with sterilized soil (July 2023) and non-sterilized soil (December 2023) respectively.

[0083] For the first pot experiment, soil samples were taken from the farm of Inner Mongolia Agricultural University, sterilized at 180 °C for 2 h in an oven, and then mixed evenly with vermiculite at a volume ratio of 2:1 for use; for the second pot experiment, the soil from the new area farm of Inner Mongolia Agricultural University was taken without sterilization and used directly, and the rest was the same as the first pot experiment.

[0084] For each treatment, 6 herbicide concentrations of 0, 80, 100, 120, 140, 160 mL / 667m 2 were set, and there were 3 replicates.

[0085] After sowing, the soil was covered with 2 - 3 cm of soil. The soil surface area was calculated by the diameter of the soil in different flowerpots, and the water consumption was 60 kg per 667m 2 The herbicide concentrations were 0, 80, 100, 120, 140, 160 mL / 667m 2Six different application concentrations were used. The water consumption and drug application amount in the flower pot were calculated according to the soil surface area in the flower pot, and then the herbicide Pendimethalin was sprayed randomly. Ten days after sowing, the phytotoxicity level was investigated according to the phytotoxicity grading standard (Table 5), and the phytotoxicity index and the ratio of reducing phytotoxicity were calculated.

[0086] Table 5 Phytotoxicity grading standard of Pendimethalin for buckwheat

[0087]

[0088] Note: During the investigation, record the number of buckwheat seedlings damaged and the damage level, and calculate the phytotoxicity index and phytotoxicity control effect.

[0089]

[0090] The results are shown in Tables 6, 7, 8, and 9.

[0091] Table 6 Phytotoxicity index of buckwheat after seed dressing with strains and spraying Pendimethalin (July 2023, soil sterilized)

[0092]

[0093] Table 7 Ratio of reducing phytotoxicity of buckwheat after seed dressing with strains and spraying Pendimethalin (%) (July 2023, soil sterilized)

[0094]

[0095] Table 8 Phytotoxicity index of buckwheat after seed dressing with strains and spraying Pendimethalin (December 2023, soil not sterilized)

[0096]

[0097] Table 9 Ratio of reducing phytotoxicity of buckwheat after seed dressing with strains and spraying Pendimethalin (%) (December 2023, soil not sterilized)

[0098]

[0099] As can be seen from Tables 6 and 7, under the condition of soil sterilization, when applying herbicides at 80, 100, 120, 140, 160 mL / 667 m 2 the strain JGZ26 could alleviate the phytotoxicity of Pendimethalin to buckwheat. Among them, when the herbicide dosage was 80 mL / 667 m 2 the alleviating effect was the best, and the reduction ratio was 64.6%. Followed by the herbicide dosage of 100 mL / 667 m 2 with a reduction ratio of 52.2%. As can be seen from Tables 9 and 10, when applying herbicides at 80, 100, 120, 140, 160 mL / 667 m 2When applied, strain JGZ26 can alleviate the phytotoxicity of pendimethalin to buckwheat. Among them, when the herbicide dosage is 80 mL / 667 m 2 The alleviation effect is the best, and the reduction ratio is 64.0%.

[0100] Example 6

[0101] Alleviation effect of strain JGZ26 on pendimethalin phytotoxicity in field trials

[0102] Buckwheat seeds were dressed with strain JGZ26, and then different concentrations of herbicides were sprayed. The effects of strain JGZ26 on degrading pendimethalin and alleviating phytotoxicity were analyzed by changes in phytotoxicity index.

[0103] The test site was set at the teaching base of Inner Mongolia Agricultural University in Hailiutu, Tumd Left Banner, Hohhot, Inner Mongolia. In the test, 0, 80, 100, 120, 140, and 160 mL / 667 m 2 Six concentrations of the herbicide pendimethalin were set for each treatment, and three replicates were set. The pretreatment method of dressing seeds with the bacterial solution was the same as in Example 5.

[0104] Sowing was carried out on July 20, 2023, with a row spacing of 40 cm. A furrow with a depth of 4 - 5 cm was opened with a furrow opener. The seeds dressed with the bacterial solution were sown and immediately covered with soil, and then different concentrations of the soil - closed herbicide pendimethalin were applied immediately. The herbicide was diluted according to 60 kg of water consumption per 667 m 2 and 0, 80, 100, 120, 140, 160 mL / 667 m 2 Six different application concentrations. 15 days after sowing, the phytotoxicity level was investigated according to the phytotoxicity grading standard (Table 5), and the phytotoxicity index and the ratio of alleviating phytotoxicity were calculated. The calculation formulas were the same as in Example 5.

[0105] The effect of strain JGZ26 on alleviating buckwheat seedling phytotoxicity can be seen in Table 10 and Table 11.

[0106] Table 10 Phytotoxicity index of buckwheat after spraying pendimethalin with strain dressing in field trials

[0107]

[0108] Table 11 Effect of strain dressing on alleviating pendimethalin phytotoxicity to buckwheat in field trials (%)

[0109]

[0110] As can be seen from Table 10 and Table 11, in the field trial, when the herbicide dosages were 80, 100, 120, 140, 160 mL / 667 m 2 strain JGZ26 could alleviate the phytotoxicity of pendimethalin to buckwheat. Among them, when the herbicide dosage was 120 mL / 667 m 2The alleviation effect is the best, with a reduction ratio of 73.9%. Next is when the herbicide dosage is 80 and 100 mL / 667m 2 , and the reduction ratios are 47.2% and 52.2% respectively.

[0111] Example 7

[0112] Effect of strain JGZ26 on the yield and 1000-grain weight of buckwheat in field trials

[0113] Buckwheat seeds were dressed with strain JGZ26, and then different concentrations of herbicides were sprayed. The yield increase effect of the isolated strain was analyzed through yield and 1000-grain weight.

[0114] The test site was set at the teaching base of Inner Mongolia Agricultural University in Hailiutu, Tumote Left Banner, Hohhot, Inner Mongolia. In the test, each treatment had 6 concentrations of the herbicide S-metolachlor at 0, 80, 100, 120, 140, 160 mL / 667m 2 and one manual weeding control, with 3 replicates. The pretreatment method of dressing the seeds with the bacterial solution was the same as in Example 5.

[0115] Sowing was carried out on July 20, 2023. The row spacing was set at 40 cm. A ridging tool was used to open ridges with a depth of 4 - 5 cm. The seeds dressed with the bacterial solution were sown and immediately covered with soil, and then different concentrations of the soil-sealing herbicide S-metolachlor were immediately applied. The herbicide was diluted according to 60 kg of water consumption per 667m 2 and 6 different application concentrations of the herbicide at 0, 80, 100, 120, 140, 160 mL / 667m 2 After harvesting, the yield and 1000-grain weight were measured. The yield increase effect of strain JGZ26 is shown in Tables 12 and 13.

[0116] Table 12 Effect of strain dressing on the yield of buckwheat after spraying S-metolachlor in field trials (kg / 667m 2 )

[0117]

[0118] Table 13 Effect of strain dressing on the 1000-grain weight of buckwheat after spraying S-metolachlor in field trials (g)

[0119]

[0120] As can be seen from Table 12, the yield with the application of the microbial agent JGZ26 is greater than that without the application of the microbial agent CK at each herbicide concentration. When no microbial agent CK was applied, when the dosage of the herbicide S-metolachlor was 100 mL / 667m 2 , the yield was 84.1 kg / 667m 2 , and the yield of the manual weeding control was 85.2 kg / 667m 2There was no significant difference, indicating that when the dosage of Pendimethalin was 100 mL / 667 m 2 , the yield was not reduced. When the dosage of Pendimethalin was greater than 100 mL / 667 m 2 , the yield was significantly lower than that of the manual weeding control, indicating that a herbicide dosage greater than 100 mL / 667 m 2 would reduce the buckwheat yield. When applying the microbial agent JGZ26, when the dosage of the herbicide Pendimethalin was 100 or 120 mL / 667 m 2 , there was no significant difference from the manual weeding, indicating that when the dosage of Pendimethalin was 100 or 120 mL / 667 m 2 , the yield was not reduced.

[0121] As can be seen from Table 13, when not using the microbial agent CK and the dosage of the herbicide Pendimethalin was 80 mL / 667 m 2 , the 1000-grain weight was 22.2 g, and there was no significant difference from the 1000-grain weight of 22.6 g in the manual weeding control, indicating that when the dosage of Pendimethalin was 80 mL / 667 m 2 , it was safe for buckwheat and did not reduce the 1000-grain weight. When the dosage of Pendimethalin was greater than 80 mL / 667 m 2 , the 1000-grain weight was significantly lower than that of the manual weeding control, indicating that a herbicide dosage greater than 80 mL / 667 m 2 would reduce the buckwheat 1000-grain weight. When using the JGZ26 microbial agent, the 1000-grain weight was greater than that without using the microbial agent CK, indicating that the JGZ26 microbial agent could increase the 1000-grain weight. When the dosage of Pendimethalin was greater than 140 mL / 667 m 2 , the 1000-grain weight was significantly reduced compared with other herbicide concentrations, indicating that when the herbicide dosage was greater than 140 mL / 667 m 2 , the ability of the microbial agent JGZ26 to alleviate the reduction of the 1000-grain weight caused by Pendimethalin decreased.

[0122] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the embodiments, reference can be made to each other.

[0123] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A strain of Bacillus thuringiensis JGZ26, characterized in that: The Bacillus thuringiensis was named JGZ26, and its classification name was Bacillus thuringiensis. It was deposited in the China Center for Type Culture Collection on May 22, 2023, with the deposit number CCTCC NO: M 2023806, and the deposit address is Wuhan University, Wuhan, China.

2. Use of the Bacillus thuringiensis JGZ26 according to claim 1 in degrading the herbicide Jindul.

3. Use of the Bacillus thuringiensis JGZ26 according to claim 1 in alleviating Jindul pesticide damage to plants.

4. Use of the Bacillus thuringiensis JGZ26 according to claim 1 in increasing plant yield and thousand-grain weight.

5. The use according to any one of claims 3 or 4, characterized in that: The plant is buckwheat.

6. A herbicide Jindul degrading bacterial agent, characterized in that: Including the Bacillus thuringiensis JGZ26 described in claim 1.

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