Bacillus velezensis and application thereof
By using Bacillus Bacillus Belère WXB10 to degrade the herbicide Jinduer, the problem of Jinduer in buckwheat fields was solved, and the protection and yield of buckwheat was improved, while avoiding environmental pollution.
Patent Information
- Application Number
- CN202510233998.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2025-05-30
AI Technical Summary
The existing technology has failed to effectively solve the drug damage problem of the herbicide Jinduer in buckwheat fields, especially the lack of reliable herbicides during the seedling stage and after seedling emergence, resulting in drug damage and environmental pollution.
A Bacillus Veles WXB10 is used to reduce the herbicide Jinduer through its ability to degrade the buckwheat medicine, and increase crop yield and 1,000 grain weight.
Bacillus Veles WXB10 can degrade 49.62% and 63.41% of Jindul in 7 days and 21 days respectively, significantly alleviating the harm of buckwheat, without pollution to humans and animals and the environment, and has good application prospects.
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Figure CN120060044A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of pendimethalin degradation, and more specifically to a Bacillus velezensis strain and its application. Background Art
[0002] Weeds in buckwheat fields are the main factors affecting buckwheat yield. At present, agricultural and mechanical measures cannot be fully utilized for prevention and control, mainly relying on chemical herbicides. However, buckwheat is particularly sensitive to herbicides. When using herbicides for controlling gramineous weeds at the seedling stage, the dosage must be strictly controlled. For broad-leaved weeds in buckwheat fields, there are no available herbicides after emergence, and only individual herbicides in amide herbicides, such as pendimethalin (S - metolachlor), can be used for soil sealing treatment. However, when farmers use it, they often cannot accurately measure the size of the field, accurately weigh the dosage of the drug and the amount of water used, often resulting in phytotoxicity. There is no research on the prevention and control of herbicide phytotoxicity in buckwheat fields at home and abroad, and there is a lack of measures to solve the problems of buckwheat phytotoxicity and environmental hazards in the planting link.
[0003] The method of using microorganisms such as bacteria and fungi to alleviate herbicide phytotoxicity and degrade herbicides is safe and pollution - free, which is beneficial to improving crop quality, yield and safety. However, at present, strains for degrading the herbicide pendimethalin have not been reported.
[0004] In summary, how to provide a degradation bacterium of the herbicide pendimethalin to alleviate the phytotoxicity of pendimethalin to buckwheat is an urgent problem to be solved by those skilled in the art. Summary of the Invention
[0005] In view of this, the present invention provides a Bacillus velezensis strain and its application.
[0006] In order to achieve the above - mentioned purpose, the present invention adopts the following technical scheme:
[0007] A Bacillus velezensis strain WXB10, named WXB10, its taxonomic name is Bacillus velezensis, and it was deposited in the China Center for Type Culture Collection on May 19, 2022, with the deposit number CCTCC NO: M 2022680, and the deposit address is Wuhan University, Wuhan, China.
[0008] The application of the above - mentioned Bacillus velezensis strain WXB10 in degrading the herbicide pendimethalin.
[0009] The application of the above - mentioned Bacillus velezensis strain WXB10 in alleviating pendimethalin phytotoxicity to plants.
[0010] The application of the above - mentioned Bacillus velezensis strain WXB10 in increasing plant yield and 1000 - grain weight.
[0011] Further, the plant is buckwheat.
[0012] A herbicide pendimethalin degrading bacterium agent, including the above-mentioned Bacillus velezensis WXB10.
[0013] As can be seen from the above technical solutions, compared with the prior art, the beneficial effects achieved by the present invention are as follows:
[0014] The pendimethalin degrading bacterium of the present invention is Bacillus velezensis WXB10. Bacillus velezensis WXB10 degrades pendimethalin in the medium for 7 days, and the degradation rate is 49.62%. It degrades pendimethalin in the medium for 21 days, and the degradation rate is 63.41%. The Bacillus velezensis WXB10 provided by the present invention is used for rapid degradation of pendimethalin, alleviates the phytotoxicity of buckwheat seedlings at the seedling stage, is safe for humans and animals, and has no pollution to the environment, and has good development and application prospects. BRIEF DESCRIPTION OF THE DRAWINGS
[0015] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required in the description of the embodiments or the prior art. Obviously, the drawings in the following description are only the embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained according to the provided drawings.
[0016] Figure 1 It is the result diagram of the plate test in Embodiment 2 of the present invention;
[0017] Figure 2 It is the strain morphology diagram of strain WXB10 in Embodiment 4 of the present invention;
[0018] Figure 3 It is the Gram staining diagram of strain WXB10 in Embodiment 4 of the present invention;
[0019] Figure 4 It is the phylogenetic tree of strain WXB10 in Embodiment 4 of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0021] The agents required for the present invention are conventional experimental agents, purchased from commercial channels; the experimental methods not mentioned are conventional experimental methods, which will not be elaborated here one by one.
[0022] The components of the culture media used in the following examples are as follows:
[0023] LB solid medium: 10 g of peptone, 5 g of yeast extract powder, 10 g of NaCl, 15 g of agar, 1000 mL of distilled water.
[0024] LB liquid medium: 10 g of peptone, 5 g of yeast extract powder, 10 g of NaCl, 1000 mL of distilled water.
[0025] PDA medium: 200 g of peeled potatoes, 18 g of agar, 20 g of glucose, 1000 mL of distilled water.
[0026] Basic inorganic salt medium: KH 2 PO 4 1.6 g, K 2 HPO 4 0.52 g, NH 4 NO 3 1.0 g, 0.5 g of NaCl, MgSO 4 ·7H 2 O 0.5 g, 1000 mL of distilled water.
[0027] Basic medium for carbon source determination: KH 2 PO 4 2.38 g, K 2 HPO 4 ·3H 2 O 5.65 g, (NH 4 ) 2 SO 4 2.64 g, MgSO 4 ·7H 2 O 1 g, CuSO 4 ·5H 2 O 6.4 mg, ZnSO 4 ·7H 2 O 1.5 mg, FeSO 4 ·7H 2 O 1.1 mg, MnCl 2 ·7H 2 O 7.9 mg, 15 g of agar, 1000 mL of distilled water.
[0028] Basic medium for nitrogen source determination: 10 g of glucose, K 2 HPO 4 ·3H 2 O 1 g, MgSO 4 ·7H 2 O 5 g, 5 g of NaCl, FeSO 4·7H 2 O 10 mg, agar 15 g, distilled water 1000 mL.
[0029] Malonate test medium: Sodium malonate 3 g, yeast extract 1 g, NaCl 2 g, (NH 4 ) 2 SO 4 2 g, KH 2 PO 4 0.4 g, K 2 HPO 4 ·3H 2 O 0.6 g, bromothymol blue 25 mg, distilled water 1000 mL, pH 7.4.
[0030] Methyl red test medium: Peptone 7.0 g, glucose 5 g, NaCl 5 g, distilled water 1000 mL.
[0031] Starch hydrolysis test medium: Soluble starch 2 g, beef extract 3 g, peptone 5 g, glucose 2.5 g, agar 18 g, distilled water 1000 mL, pH 7.0.
[0032] Fat hydrolysis test medium: Peptone 10 g, CaCl 2 ·2H 2 O 0.1 g, agar 17 g, distilled water 1000 mL, pH 7.4.
[0033] NA medium: Beef extract 3 g, peptone 5 g, glucose 2.5 g, agar 18 g, distilled water 1000 mL, pH 7.0.
[0034] Example 1
[0035] Isolation of strains
[0036] Soil samples were taken from the soil layer 0 - 10 cm from the surface of a plot in Wuchuan County, Hohhot City, Inner Mongolia. Weigh 5 g of soil sample and place it in a liquid basic inorganic salt culture medium containing 95 mL. Add pendimethalin as the sole carbon source for microbial growth and utilization, so that the final concentration of pendimethalin is 50 mg / L. Incubate on a shaker at 150 r / min and 28 °C for 7 days. Take 1 mL and transfer it to a basic inorganic salt culture medium containing 100 mg / L pendimethalin and incubate for 7 days. Then take 1 mL and transfer it to a basic inorganic salt culture medium containing 150 mg / L pendimethalin and incubate for 7 days. By gradually increasing the concentration of pendimethalin, the selection pressure of the herbicide on the microorganisms was increased, aiming to isolate and screen highly efficient herbicide-degrading strains. After 21 days of enrichment culture, the basic inorganic salt culture medium was made into 10 -1 、10 -3 、10 -4 、10 -5At different concentrations, 200 μL of culture media at different concentrations were respectively spread on PDA and LB media. 10 -3 concentration of the culture medium was spread on PDA, and 10 -4 and 10 -5 concentration of the culture medium were spread on LB for the isolation of fungi and bacteria. It was repeated 3 times. After inoculation, it was placed in an incubator at 25 °C for 2 - 5 days. Single colonies with different morphologies were picked for purification, numbered, stored, and reserved for use.
[0037] Example 2
[0038] Primary screening of pendimethalin-degrading bacteria by plate method
[0039] The isolated bacterial strains were secondarily purified on LB medium by the streak plate method, and the fungal strains were secondarily purified on PDA medium by the single spore isolation method. The purified bacterial strains were spread on LB medium by the dilution plating method and cultured for 3 days, and the fungal strains were inoculated on PDA medium and cultured for 5 days. 500 μL of the herbicide pendimethalin was quickly added to 99.5 mL of the basal inorganic salt medium at a temperature of 40 ± 2.5 °C to make the total volume of the solution in the medium 100 mL, and it was immediately mixed evenly. Before the medium solidified, it was poured into glass petri dishes, 20 mL per dish. After the medium solidified in the petri dishes, bacterial and fungal disks with a diameter of 5 mm were inverted and inoculated into the petri dishes. The non-inoculated petri dishes were used as controls. Each treatment was repeated 3 times and cultured at 25 °C. Observe whether there is a clear zone around the disks. After culturing for 7 days, measure the diameter of the clear zone to preliminarily judge whether the strain can utilize the herbicide pendimethalin. The results are shown in Table 1, Figure 1 as shown.
[0040] Table 1 Results of plate test
[0041]
[0042] After primary screening, 1 strain was obtained and named WXB10. Strain WXB10 showed a clear zone on the plate, and the diameter D of the clear zone was 1.54 cm, indicating its ability to utilize the herbicide pendimethalin.
[0043] Example 3
[0044] Determination of the pendimethalin degradation rate of strain WXB10
[0045] Method for determining the pendimethalin degradation rate: Take a concentration of 1×10 85 mL of the bacterial suspension to be tested with a concentration of CFU / mL was aseptically transferred into 95 mL of a basal inorganic salt medium with a pendimethalin concentration of 100 mg / L, and incubated at a constant temperature in a shaker at 25 °C and 180 r / min for 7 d and 21 d to obtain the test solution. 5 mL of the test solution was placed in a 50 mL centrifuge tube, 5 mL of acetonitrile was added, and the mixture was shaken on a shaker at 25 °C and 220 r / min for 1 h. 1 g of NaCl was added and shaken until obvious liquid layering was observed. Then, it was centrifuged at 4000 g for 5 min, and the upper organic liquid was aspirated with a pipette. The supernatant was filtered through a 0.22 μm disposable filter, and the degradation rate was determined using a high-performance liquid chromatograph after filtration.
[0046] Instrument conditions: The concentration of S - metolachlor in the sample was determined by liquid chromatography. The mobile phase was acetonitrile: ultrapure water (20:80, V / V). The detection wavelength of S - metolachlor was 254 nm, the flow rate was 1.0 mL / min, the injection volume was 5 μL, the column temperature was 25 °C, and the retention time was 8 min. The substance was determined according to its elution time, and the content of S - metolachlor was quantitatively calculated according to its peak area.
[0047]
[0048] The results are shown in Table 2.
[0049] Table 2 Degradation rate results of the isolated strains on S - metolachlor
[0050]
[0051]
[0052] Example 4
[0053] Identification of strain WXB10
[0054] (1) Morphological identification
[0055] The strain was spread on an LB solid medium by the dilution method to grow single colonies. The single colonies of strain WXB10 were milky white, with a dry and rough surface, wrinkles, opaque, and irregular edges ( Figure 2 ).
[0056] (2) Physiological and biochemical identification
[0057] The test referred to the Manual of Common Bacterial System Identification, and the physiological and biochemical indexes of the strain, such as the utilization of carbon sources, nitrogen sources, malonate, methyl red test, starch hydrolysis, oxidase test, and catalase test, were determined. Strain WXB10 was a Gram - positive bacterium ( Figure 3) Mannitol, fructose, glucose, histidine, ammonium nitrate, and ammonium sulfate can be used as the sole carbon and nitrogen sources. It can hydrolyze starch and fat, utilize malonate, and produce catalase. See Table 3 for details.
[0058] Table 3 Physiological and Biochemical Characteristics of Strain WXB10
[0059]
[0060] Note: "+" indicates positive, and "-" indicates negative.
[0061] (3) Molecular Biology Identification
[0062] DNA extraction was carried out according to the instructions of the TIAN GEN Bacterial Genomic DNA Extraction Kit (centrifugal column type) (Beijing, China), purchased from Tiangen Biochemical Technology (Beijing) Co., Ltd. The PCR primers for strain WXB10 were 27F and 1492R, 7F and 1540R, rpoBF and rpoBR, trpBF and trpBR, respectively. The primer sequences are shown in Table 4.
[0063] The 25 μL reaction system contained: 17 μL of ddH 2 O, 2.5 μL of 10×PCR Buffer, 2 μL of dNTP, 1 μL of the upstream primer, 1 μL of the downstream primer, 0.5 μL of Taq DNA polymerase, and 1 μL of template DNA. PCR reaction conditions: pre-denaturation at 94 °C for 5 min; denaturation at 94 °C for 30 s; annealing temperature for 30 s; extension at 72 °C for 1 min; 34 cycles; extension at 72 °C for 10 min.
[0064] The PCR products of the 4 genes were detected by 1% agarose gel electrophoresis and sent to Shanghai Sangon Biotech Co., Ltd. for 16S rDNA sequencing. The sequencing results are as follows. The 4 gene sequences obtained were spliced, and the splicing results were compared with the GenBank nucleic acid database. The strain sequences with higher similarity were selected for analysis, and the phylogenetic tree was constructed using the software MEGA7.0. Strain WXB10 clustered with Bacillus velezensis ( Figure 4 ).
[0065] Table 4 Primers and Sequences
[0066]
[0067] The amplified sequence of WXB10 is as follows:
[0068] 27F 1492R:
[0069]
[0070] 7F 1540R:
[0071]
[0072] rpoBR rpoBF:
[0073]
[0074] trpBF trpBR:
[0075]
[0076] Based on the results of comprehensive morphological, physiological and biochemical tests and molecular biological identification, the strain WXB10 was identified as Bacillus velezensis.
[0077] (4) Preservation
[0078] The Bacillus velezensis was named WXB10, and its taxonomic name is Bacillus velezensis. It was preserved in the China Center for Type Culture Collection on May 19, 2022, with the preservation number CCTCC NO: M 2022680, and the preservation address is Wuhan University, Wuhan, China.
[0079] Example 5
[0080] Mitigation effect of strain WXB10 on pendimethalin phytotoxicity in pot experiments
[0081] Strain WXB10 was used for seed dressing of buckwheat, and then different concentrations of herbicides were sprayed. The effects of strain WXB10 on degrading pendimethalin and mitigating phytotoxicity were analyzed through the change of phytotoxicity index.
[0082] Strain WXB10 was cultured in LB liquid medium in a shaker at 25 °C and 180 r / min, and diluted to 1×10 8 CFU / mL to prepare a bacterial agent; for seed dressing of buckwheat, 20 mL of the bacterial agent was added to every 20 g of seeds and stirred evenly, and then placed in a cool and dry place to dry for later use.
[0083] There were two pot experiments in total: one was carried out with sterilized soil (July 2023) and the other with non-sterilized soil (December 2023).
[0084] For the first pot experiment, soil samples were taken from the farm of Inner Mongolia Agricultural University, sterilized at 180 °C for 2 h in an oven, and then mixed evenly with vermiculite at a volume ratio of 2:1 for use; for the second pot experiment, the soil from the new area farm of Inner Mongolia Agricultural University was used directly without sterilization, and the rest was the same as the first pot experiment.
[0085] For each treatment, 6 herbicide concentrations of 0, 80, 100, 120, 140, 160 mL / 667m 2 were set, and there were 3 replicates.
[0086] After sowing, the soil was covered with 2 - 3 cm of soil. The soil surface area was calculated based on the diameter of the soil in different flower pots. According to the water consumption of 60 kg per 667m 2 and herbicides of 0, 80, 100, 120, 140, 160 mL / 667m 2Six different application concentrations were used. The water consumption and chemical dosage in the flowerpots were calculated according to the soil surface area in the flowerpots, and then the herbicide Pendimethalin was sprayed randomly. Ten days after sowing, the phytotoxicity level was investigated according to the phytotoxicity grading standard (Table 5), and the phytotoxicity index and the ratio of reducing phytotoxicity were calculated.
[0087] Table 5 Phytotoxicity grading standard of Pendimethalin on buckwheat
[0088]
[0089] Note: Record the number of damaged buckwheat seedlings and the damage level during the investigation, and calculate the phytotoxicity index and the control effect of phytotoxicity.
[0090]
[0091]
[0092] The results are shown in Tables 6, 7, 8, and 9.
[0093] Table 6 Phytotoxicity index of buckwheat after seed dressing with strains and spraying Pendimethalin (July 2023, soil sterilized)
[0094]
[0095] Table 7 Ratio of reducing phytotoxicity of buckwheat after seed dressing with strains and spraying Pendimethalin (%) (July 2023, soil sterilized)
[0096]
[0097] Table 8 Phytotoxicity index of buckwheat after seed dressing with strains and spraying Pendimethalin (December 2023, soil not sterilized)
[0098]
[0099] Table 9 Ratio of reducing phytotoxicity of buckwheat after seed dressing with strains and spraying Pendimethalin (%) (December 2023, soil not sterilized)
[0100]
[0101] As can be seen from Tables 6 and 7, under the condition of soil sterilization, when applying herbicides at 80, 100, 120, 140, 160 mL / 667 m 2 , strain WXB10 could relieve the phytotoxicity of Pendimethalin on buckwheat. Among them, when the herbicide dosage was 80 mL / 667 m 2 and 100 mL / 667 m 2 , the relief effect was the best, and the reduction ratios were 58.0% and 51.7%. As can be seen from Tables 8 and 9, under the condition of non-sterilized soil, when applying herbicides at 80, 100, 120, 140, 160 mL / 667 m 2When applied, strain WXB10 can alleviate the phytotoxicity of pendimethalin to buckwheat. Among them, when the herbicide dosage is 80 mL / 667 m 2 The alleviation effect is the best, and the reduction ratio is 39.8%.
[0102] Example 6
[0103] Alleviation effect of strain WXB10 on pendimethalin phytotoxicity in field trials
[0104] Buckwheat seeds were dressed with strain WXB10, and then different concentrations of herbicides were sprayed. The degradation effect of strain WXB10 on pendimethalin and the alleviation effect of phytotoxicity were analyzed by the change of phytotoxicity index.
[0105] The test site was set at the teaching base of Inner Mongolia Agricultural University in Hailiutu, Tumote Left Banner, Hohhot, Inner Mongolia. In the test, six concentrations of the herbicide pendimethalin, namely 0, 80, 100, 120, 140, and 160 mL / 667 m 2 were set for each treatment, and three replicates were set. The pretreatment method of dressing seeds with the bacterial solution was the same as that in Example 5.
[0106] Sowing was carried out on July 20, 2023, with a row spacing of 40 cm. A furrow with a depth of 4 - 5 cm was opened with a furrow opener. The seeds dressed with the bacterial solution were sown and immediately covered with soil, and then different concentrations of the soil - closed herbicide pendimethalin were applied immediately. The herbicide was diluted according to 60 kg of water consumption per 667 m 2 and six different application concentrations of 0, 80, 100, 120, 140, and 160 mL / 667 m 2 of pendimethalin. 15 days after sowing, the phytotoxicity level was investigated according to the phytotoxicity grading standard (Table 5), and the phytotoxicity index and the ratio of alleviating phytotoxicity were calculated. The calculation formulas were the same as those in Example 5.
[0107] The effect of strain WXB10 on alleviating buckwheat seedling phytotoxicity can be seen in Table 10 and Table 11.
[0108] Table 10 Phytotoxicity index of buckwheat after spraying pendimethalin with strain dressing in field trials
[0109]
[0110] Table 11 Effect of strain dressing on alleviating buckwheat phytotoxicity after spraying pendimethalin in field trials (%)
[0111]
[0112] As can be seen from Table 10 and Table 11, when the herbicide dosages are 80, 100, 120, 140, and 160 mL / 667 m 2 are applied, strain WXB10 can all alleviate the phytotoxicity of pendimethalin to buckwheat. Among them, when the herbicide dosage is 80 mL / 667 m 2The mitigation effect is the best, with a reduction ratio of 80.6%. Next is 100 mL / 667 m 2 , with a reduction ratio of 54.3%.
[0113] Example 7
[0114] Effect of strain WXB10 on the yield and 1000-grain weight of buckwheat in field trials
[0115] Buckwheat seeds were dressed with strain WXB10, and then different concentrations of herbicides were sprayed. The yield increase effect of the isolated strain was analyzed through yield and 1000-grain weight.
[0116] The test site was set at the teaching base of Inner Mongolia Agricultural University in Hailiutu, Tumd Left Banner, Hohhot, Inner Mongolia. In the test, each treatment had 6 concentrations of the herbicide Jinduer at 0, 80, 100, 120, 140, 160 mL / 667 m 2 and one manual weeding control, with 3 replicates. The pretreatment method of dressing the seeds with the bacterial solution was the same as in Example 5.
[0117] Sowing was carried out on July 20, 2023, with a row spacing of 40 cm. A furrow with a depth of 4 - 5 cm was opened with a furrow opener. The seeds dressed with the bacterial solution were sown and immediately covered with soil, and then different concentrations of the soil-sealing herbicide Jinduer were immediately applied. The herbicide was diluted according to 60 kg of water consumption per 667 m 2 and 6 different application concentrations of the herbicide at 0, 80, 100, 120, 140, 160 mL / 667 m 2 After harvesting, the yield and 1000-grain weight were measured. The yield increase effect of strain WXB10 is shown in Tables 12 and 13.
[0118] Table 12 Effect of strain dressing on the yield of buckwheat after spraying Jinduer in field trials (kg / 667 m 2 )
[0119]
[0120] Table 13 Effect of strain dressing on the 1000-grain weight of buckwheat after spraying Jinduer in field trials (g)
[0121]
[0122] As can be seen from Table 12, when the microbial agent WXB10 was applied, the yield was greater than that without applying the microbial agent CK at each herbicide dosage. Among those without applying the microbial agent CK, when the herbicide dosage was 100 mL / 667 m 2 , there was no significant difference in yield compared with the manual weeding control, indicating that when the herbicide dosage was 100 mL / 667 m 2 , it was safe for buckwheat and did not affect the yield. When the microbial agent WXB10 was applied and the herbicide dosage was 80 mL / 667 m 2, 100 mL / 667 m 2 When it is 100 mL / 667 m, there is no significant difference in yield compared with the manual weeding control, indicating that this bacterial agent can alleviate the yield reduction caused by 80 - 100 mL / 667 m 2 of pendimethalin. As can be seen from Table 13, in CK without applying the bacterial agent, when the herbicide dosage is 80 mL / 667 m 2 , there is no significant difference in 1000 - grain weight compared with the manual weeding control, indicating that when the herbicide dosage is 80 mL / 667 m 2 , it is safe for buckwheat and does not affect the 1000 - grain weight. When applying the bacterial agent WXB10 and the herbicide dosage is 80 mL / 667 m 2 , there is no significant difference in 1000 - grain weight compared with the manual weeding control.
[0123] In this specification, each embodiment is described in a progressive manner. The key point of each embodiment is to illustrate the differences from other embodiments. For the same or similar parts among the embodiments, reference can be made to each other.
[0124] The above description of the disclosed embodiments enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be obvious to those skilled in the art. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.
Claims
1. A strain of Bacillus Velezii WXB10, characterized in that: The Bacillus velezensis was named WXB10, and its classification name was Bacillus velezensis. It was deposited in the China Center for Type Culture Collection on May 19, 2022, with the deposit number CCTCC NO: M 2022680, and the deposit address is Wuhan University, Wuhan, China.
2. Use of the Bacillus Velez subtilis WXB10 described in claim 1 in degrading the herbicide Kindor.
3. Use of the Bacillus Velez subtilis WXB10 described in claim 1 in alleviating Jindul pesticide damage to plants.
4. Use of the Bacillus Velez subtilis WXB10 described in claim 1 in improving plant yield and thousand-grain weight.
5. The use according to any one of claims 3 or 4, characterized in that: The plant is buckwheat.
6. A herbicide Jindul degrading bacterial agent, characterized in that: The invention comprises the Bacillus Velez subtilis WXB10 described in claim 1.