Tea plant CsCUL1 gene, tea plant CsCUL1 protein and their applications

By regulating the CsCUL1 gene of tea plants through VIGS, the leaf color of tea plants was controlled, solving the problem of leaf color regulation and improving the quality and market competitiveness of tea.

CN120060274BActive Publication Date: 2026-05-26GUIZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUIZHOU UNIV
Filing Date
2025-01-17
Publication Date
2026-05-26

AI Technical Summary

Technical Problem

There are few existing biotechnological methods for regulating the color of tea leaves, making it difficult to effectively regulate the color and quality of tea leaves through gene regulation.

Method used

The CsCUL1 gene and its encoded CsCUL1 protein in tea plants were discovered through screening and VIGS technology. The CsCUL1 gene was silenced or overexpressed using VIGS gene silencing technology to regulate the leaf color phenotype of tea plants and change the content of chlorophyll and carotenoids.

Benefits of technology

This technology enables effective regulation of tea tree leaf color, improves the photosynthetic efficiency and quality of tea leaves, and allows for the production of tea varieties with different leaf colors to meet market demand.

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Abstract

This invention relates to a tea plant CsCUL1 gene, a tea plant CsCUL1 protein, and their applications. The CDS nucleotide sequence of the tea plant CsCUL1 gene is shown in SEQ ID NO.1, and the amino acid sequence of the encoded tea plant CsCUL1 protein is shown in SEQ ID NO.2. This invention utilizes VIGS gene silencing technology and vacuum infection to verify the function of the CsCUL1 gene. The experimental material was one-year-old branches of the Longjing 43 tea plant. The results showed that the chlorophyll and carotenoid content in the leaves of tea plants with silenced CsCUL1 was significantly reduced. This invention can be applied to molecular genetic breeding of tea plant leaves with different leaf colors, which is helpful for the regulation and selection of tea plant varieties with different leaf colors in production and has important significance for improving the sensory quality of tea products.
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