Photochemical analysis test strip structure

Abstract

The invention provides a photochemical analysis test strip structure which comprises a test strip body, a sample introduction area, a sample introduction flow channel and a reaction detection area which are sequentially communicated are arranged in the test strip body, and a sample introduction port is formed in the test strip body; a hydrophobic layer is arranged at the bottom of the reaction detection area, a notch and a guide hole which are sequentially communicated are formed in the hydrophobic layer, and the guide hole and the reaction detection area are concentrically arranged; the part, located above the hydrophobic layer, of the reaction detection area is an edge area, the other part is a detection center area, and a reagent is arranged in the detection center area; a plurality of first air holes communicated with the edge area are formed in the test strip body. Through the arrangement of the hydrophobic layer, a sample entering the reaction detection area preferentially fills the detection center area and then diffuses to the edge area, the sample is guided to flow orderly, and bubbles are forced to migrate to the edge area and are discharged from the first air hole in the flowing process of the sample, so that the bubble interference of the detection center area is effectively eliminated, and the detection accuracy is further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of body fluid analysis, and particularly to a structure of a photochemical analysis test strip. Background Art

[0002] A photochemical analysis test strip is a rapid diagnostic tool based on the principle of optical detection. By reacting a specific chemical reagent loaded on the test strip with the target substance, color change, fluorescence or chemiluminescence signals are generated, and combined with an optical detection instrument (such as a spectrophotometer, a fluorescence detector) to achieve detection and analysis.

[0003] For example, a Chinese patent application with the publication number CN119574538A discloses a blood lipid test card based on the photochemical principle, including a card body. A sample adding port, a calibration area and at least one test area are provided on the card body. The sample adding port is respectively communicated with the calibration area and the test area. Air holes communicating with the outside are provided in both the calibration area and the test area. A reaction film and a hemolytic agent are provided in the calibration area. An enzyme layer corresponding to the item to be detected and an anti-interference film are provided in the test area. The card body includes a bottom plate and an upper cover that can be attached together. A plurality of grooves are provided on the bottom plate, and the plurality of grooves correspondingly form the calibration area and the test area. The cover plate is provided with a notch and a plurality of flow channels. The notch forms the sample adding port. One ends of the plurality of flow channels are all connected to the notch, and the other ends of the plurality of flow channels are respectively connected to the plurality of grooves. When the above-mentioned prior art test card is in use, the sample flows into the test area through the sample adding port and the flow channels, and the air bubbles in the sample in the test area are not easily discharged, affecting the accuracy of detection.

[0004] Therefore, the prior art still needs to be improved and developed. Summary of the Invention

[0005] The purpose of the present invention is to provide a structure of a photochemical analysis test strip in view of the defects and deficiencies of the prior art.

[0006] In order to achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0007] The present invention provides a structure of a photochemical analysis test strip, including a test strip body. An injection area, an injection flow channel and a reaction detection area are sequentially communicated in the test strip body along the direction of sample flow. A sample injection port communicating with the injection area is provided on the test strip body.

[0008] A hydrophobic layer is provided at the bottom of the reaction detection area. A notch and a guiding hole are sequentially communicated along the direction of sample flow on the hydrophobic layer. The guiding hole is concentrically arranged with the reaction detection area. The part of the reaction detection area above the hydrophobic layer is the edge area, and the rest is the detection central area. A reagent is provided in the detection central area.

[0009] A plurality of first air holes communicating with the edge area are provided on the test strip body.

[0010] It can be understood that the reagent can be attached to the detection center through processes such as freeze-drying and screen printing deposition.

[0011] Through the setting of the hydrophobic layer, the sample entering the reaction detection area preferentially fills the detection center area and then diffuses to the edge area, guiding the orderly flow of the sample. During the flow process of the sample, the bubbles are forced to migrate to the edge area and are discharged to the outside through the first air holes, thereby effectively eliminating the bubble interference in the detection center area and further improving the detection accuracy.

[0012] According to the above solution, the plurality of first air holes are evenly distributed above the edge area.

[0013] According to the above solution, the first air hole is set as a tapered hole, and the side with a smaller aperture of the first air hole faces the outside. Through the above setting, it is beneficial to improve the speed of gas discharging to the outside.

[0014] According to the above solution, the sample injection flow channel is set as a serpentine shape.

[0015] The sample injection flow channel connecting the sample injection area and the reaction detection area is set as a serpentine shape. The continuous bending design of the serpentine shape will cause frequent changes in the flow direction of the sample entering the sample injection flow channel, thereby increasing the speed of the sample flowing into the reaction detection area and promoting the mixing efficiency of the reagent and the sample.

[0016] According to the above solution, the outlet of the sample injection area is communicated with the first end of the sample injection flow channel, and the outlet of the sample injection area is set as a tapered structure facing the sample injection flow channel.

[0017] Through the above structural setting, it helps the sample to gradually accelerate when flowing from the outlet of the sample injection area into the sample injection flow channel, so that the sample enters the sample injection flow channel more smoothly and effectively avoids the formation of bubbles.

[0018] According to the above solution, the cone angle α of the tapered structure is 5 - 30°. By controlling the cone angle within 5 - 30°, it not only ensures that the sample can obtain sufficient acceleration when flowing out, but also avoids the increase in flow resistance caused by too large a cone angle.

[0019] According to the above solution, the test strip body includes a light-transmitting film, a double-sided adhesive layer, and a hydrophilic film sequentially arranged from bottom to top, and the hydrophobic layer is arranged between the light-transmitting film and the double-sided adhesive layer;

[0020] The double-sided adhesive layer is provided with a sample injection hole, a sample injection guide groove and a detection hole which are connected in sequence, and the sample injection port is formed on the hydrophilic membrane; the sample injection hole, the light-transmitting membrane and the hydrophilic membrane enclose the sample injection area, the sample injection guide groove, the light-transmitting membrane and the hydrophilic membrane enclose the sample injection flow channel, and the detection hole, the hydrophobic layer, the light-transmitting membrane and the hydrophilic membrane enclose the reaction detection area.

[0021] By adopting a test strip body composed of a light-transmitting membrane, a hydrophobic layer, a double-sided adhesive layer and a hydrophilic membrane which are stacked from top to bottom, it is beneficial to simplify the production process.

[0022] According to the above solution, a super-hydrophilic guiding band is provided on the bottom surface of the hydrophilic membrane, and the super-hydrophilic guiding band covers the outlet of the sample injection hole and the sample injection guide groove. Through the above structural arrangement, the sample in the sample injection area quickly flows into the sample injection flow channel under the siphon effect of the sample injection flow channel and the action of the super-hydrophilic guiding band, and quickly flows out to the reaction detection area.

[0023] According to the above solution, the test strip body further includes a cover film arranged on the bottom surface of the light-transmitting membrane, and a light-transmitting hole corresponding to the detection hole is formed on the cover film. By providing the cover film to play a supporting role, it is beneficial to improve the mechanical properties of the test strip body.

[0024] According to the above solution, a waste liquid storage area is further provided in the test strip body, and the waste liquid storage area is communicated with the reaction detection area and is located downstream of the reaction detection area; a second air hole communicated with the reaction detection area is provided on the test strip body. The waste liquid storage area is used to store the waste liquid after the reaction to prevent backflow from affecting the detection accuracy.

[0025] The beneficial effects of the present invention are as follows:

[0026] The present invention is provided with a hydrophobic layer at the bottom of the reaction detection area. Through the setting of the hydrophobic layer, the sample entering the reaction detection area preferentially fills the detection center area and then diffuses to the edge area, guiding the sample to flow orderly. During the flowing process of the sample, the bubbles are forced to migrate to the edge area and are discharged to the outside through the first air hole, thereby effectively eliminating the bubble interference in the detection center area and further improving the detection accuracy. Description of the Drawings

[0027] Figure 1 is a structural schematic diagram of the present invention;

[0028] Figure 2 is an exploded structural schematic diagram of the present invention;

[0029] Figure 3 is a structural schematic diagram of the hydrophobic layer of the present invention;

[0030] Figure 4 is a structural schematic diagram of the double-sided adhesive layer of the present invention;

[0031] Figure 5 It is a schematic structural diagram of the hydrophilic membrane described in the present invention.

[0032] In the figure: 1. Test strip body; 11. Sampling area; 111. Tapered structure; 12. Sampling flow channel; 13. Reaction detection area; 131. Detection center area; 132. Edge area; 14. Waste liquid storage area; 2. Cover film; 21. Light-transmitting hole; 3. Light-transmitting film; 4. Hydrophobic layer; 41. Notch; 42. Guide hole; 5. Double-sided adhesive layer; 51. Sampling hole; 52. Sampling guide groove; 53. Detection hole; 6. Hydrophilic membrane; 61. Sampling port; 62. First air hole; 63. Super-hydrophilic guiding band; 64. Second air hole. Specific embodiments

[0033] The technical solution of the present invention will be described below with reference to the accompanying drawings and embodiments.

[0034] As Figures 1-5 shown, the present invention provides a photochemical analysis test strip structure, including a test strip body 1, an internal sampling area 11, a sampling flow channel 12 and a reaction detection area 13 that are sequentially connected along the direction of sample flow are arranged in the test strip body 1, and a sampling port 61 communicating with the sampling area 11 is arranged on the test strip body 1; a hydrophobic layer 4 is arranged at the bottom of the reaction detection area 13, and a notch 41 and a guide hole 42 that are sequentially connected along the direction of sample flow are formed on the hydrophobic layer 4, and the guide hole 42 is concentrically arranged with the reaction detection area 13; the part of the reaction detection area 13 located above the hydrophobic layer 4 is the edge area 132, and the rest is the detection center area 131, and a reagent is arranged in the detection center area 131; a plurality of first air holes 62 communicating with the edge area 132 are arranged on the test strip body 1.

[0035] In this embodiment, hemoglobin is taken as an example for detection, the reagent contains a hemolytic agent, and the reagent is attached to the detection center area 131 by a freeze-drying process.

[0036] Through the setting of the hydrophobic layer 4, the sample entering the reaction detection area 13 preferentially fills the detection center area 131 and then diffuses to the edge area 132, guiding the sample to flow orderly. During the flow process of the sample, the bubbles are forced to migrate to the edge area 132 and discharged to the outside through the first air holes 62, thereby effectively eliminating the bubble interference in the detection center area 131 and further improving the detection accuracy.

[0037] Furthermore, a plurality of the first air holes 62 are evenly distributed above the edge area 132.

[0038] Furthermore, the first air hole 62 is set as a tapered hole, and the side with a smaller aperture of the first air hole 62 faces the outside. Through the above setting, it is beneficial to improve the speed of gas discharging to the outside.

[0039] Furthermore, the sample injection flow channel 12 is arranged in a serpentine shape.

[0040] The sample injection flow channel 12 connecting the sample injection area 11 and the reaction detection area 13 is arranged in a serpentine shape. The continuous bending design of this serpentine shape will cause frequent changes in the flow direction of the sample entering the sample injection flow channel 12, thereby increasing the speed of the sample flowing into the reaction detection area 13 and promoting the mixing efficiency of the reagent and the sample.

[0041] Furthermore, the outlet of the sample injection area 11 is communicated with the first end of the sample injection flow channel 12, and the outlet of the sample injection area 11 is arranged as a tapered structure 111 facing the sample injection flow channel 12.

[0042] Through the above structural arrangement, it helps the sample to gradually accelerate when flowing from the outlet of the sample injection area 11 into the sample injection flow channel 12, so that the sample enters the sample injection flow channel 12 more smoothly and effectively avoids the formation of bubbles.

[0043] Furthermore, the cone angle α of the tapered structure 111 is 5 - 30°. By controlling the cone angle α within 5 - 30°, it not only ensures that the sample can obtain sufficient acceleration when flowing out, but also avoids the increase in flow resistance caused by too large a cone angle.

[0044] Furthermore, the test strip body 1 includes a light-transmitting film 3, a double-sided adhesive layer 5, and a hydrophilic film 6 arranged in sequence from bottom to top. The hydrophobic layer 4 is arranged between the light-transmitting film 3 and the double-sided adhesive layer 5; an injection hole 51, an injection guide groove 52, and a detection hole 53 are sequentially communicated on the double-sided adhesive layer 5, and an injection port 61 is opened on the hydrophilic film 6; the injection hole 51, the light-transmitting film 3, and the hydrophilic film 6 enclose the sample injection area 11, the injection guide groove 52, the light-transmitting film 3, and the hydrophilic film 6 enclose the sample injection flow channel 12, and the detection hole 53, the hydrophobic layer 4, the light-transmitting film 3, and the hydrophilic film 6 enclose the reaction detection area 13.

[0045] In this embodiment, a screen printing method is used to coat the hydrophobic ink on the light-transmitting film 3, so that a hydrophobic layer 4 with a notch 41 and a guiding hole 42 is formed on the light-transmitting film 3. By using the light-transmitting film 3, the hydrophobic layer 4, the double-sided adhesive layer 5, and the hydrophilic film 6 which are stacked from top to bottom to form the test strip body 1, it is beneficial to simplify the production process.

[0046] Furthermore, a super-hydrophilic guiding strip 63 is arranged on the bottom surface of the hydrophilic film 6, and the super-hydrophilic guiding strip 63 covers the outlet of the injection hole 51 and the injection guide groove 52. Through the above structural arrangement, the sample in the sample injection area 11 quickly flows into the sample injection flow channel 12 under the action of the siphon effect of the sample injection flow channel 12 and the super-hydrophilic guiding strip 63, and quickly flows out to the reaction detection area 13.

[0047] Furthermore, the test strip body 1 further includes a cover film 2 disposed on the bottom surface of the light-transmitting film 3, and a light-transmitting hole 21 corresponding to the detection hole 53 is formed in the cover film 2. By providing the cover film 2 to play a supporting role, it is beneficial to improve the mechanical properties of the test strip body 1.

[0048] Furthermore, a waste liquid storage area 14 is also provided in the test strip body 1. The waste liquid storage area 14 is communicated with the reaction detection area 13 and is located downstream of the reaction detection area 13; a second air hole 64 communicated with the reaction detection area 13 is provided on the test strip body 1. The waste liquid storage area 14 is used for storing the waste liquid after the reaction to prevent backflow from affecting the detection accuracy.

[0049] When the photochemical analysis test strip structure of the present invention is in use, the sample is added into the sampling area 11 from the sampling port 61. Under the siphon effect of the sampling flow channel 12 and the action of the super-hydrophilic guiding band 63, the sample quickly enters the sampling flow channel 12 from the outlet of the sampling area 11, and then is accelerated through the continuous bending design of the sampling flow channel 12 and quickly enters the reaction detection area 13 to be dissolved and mixed with the reagent; for the sample entering the reaction detection area 13, under the hydrophobic effect of the hydrophobic layer 4, it preferentially fills the detection center area 131 and then diffuses to the edge area 132, guiding the sample to flow orderly. During the flowing process of the sample, the bubbles are forced to migrate to the edge area 132 and are discharged to the outside through the first air hole 62; the sample reacts with the reagent in the reaction detection area 13, and the red blood cells in the sample are destroyed to release hemoglobin. The waste liquid after the reaction flows into the waste liquid storage area 14, and the gas in the waste liquid storage area 14 is discharged to the outside through the second air hole 64; then the test strip body 1 is placed into an optical detection instrument to irradiate and detect the sample in the detection center area 131.

[0050] The above description is only a preferred embodiment of the present invention. Therefore, all equivalent changes or modifications made according to the structures, features, and principles described in the scope of the present invention patent application are included in the scope of the present invention patent application.

Claims

1. A photochemical analysis test strip structure, comprising a test strip body (1), wherein the test strip body (1) is provided with a sample injection area (11), a sample injection channel (12) and a reaction detection area (13) which are sequentially connected along the direction of sample flow, and the test strip body (1) is provided with a sample injection port (61) which is connected with the sample injection area (11), characterized in that: A hydrophobic layer (4) is provided at the bottom of the reaction detection area (13); a notch (41) and a guide hole (42) are provided on the hydrophobic layer (4) and are connected in sequence along the direction of sample flow; the guide hole (42) is arranged concentrically with the reaction detection area (13); the portion of the reaction detection area (13) located above the hydrophobic layer (4) is an edge area (132), and the remaining portion is a detection center area (131); a reagent is arranged in the detection center area (131); The test strip body (1) is provided with a plurality of first air holes (62) which are in communication with the edge area (132).

2. The photochemical analysis test strip structure according to claim 1, characterized in that: A plurality of the first air holes (62) are evenly distributed above the edge region (132).

3. The photochemical analysis test strip structure according to claim 2, characterized in that: The first air hole (62) is configured as a conical hole, and the side of the first air hole (62) with a smaller hole diameter faces the outside.

4. The photochemical analysis test strip structure according to claim 1, characterized in that: The sample inlet channel (12) is configured in a serpentine shape.

5. The photochemical analysis test strip structure according to claim 1, characterized in that: The outlet of the injection zone (11) is in communication with the first end of the injection channel (12), and the outlet of the injection zone (11) is arranged as a tapered structure (111) facing the injection channel (12).

6. The photochemical analysis test strip structure according to claim 5, characterized in that: The taper angle α of the tapered structure (111) is 5-30°.

7. The photochemical analysis test strip structure according to any one of claims 1 to 6, characterized in that: The test strip body (1) comprises a light-transmitting film (3), a double-sided adhesive layer (5) and a hydrophilic film (6) which are arranged in sequence from bottom to top, and the hydrophobic layer (4) is arranged between the light-transmitting film (3) and the double-sided adhesive layer (5); The double-sided adhesive layer (5) is provided with a sampling hole (51), a sampling guide groove (52) and a detection hole (53) which are connected in sequence, and the hydrophilic membrane (6) is provided with the sampling port (61); The injection hole (51), the light-transmitting membrane (3) and the hydrophilic membrane (6) form the injection area (11); the injection guide groove (52), the light-transmitting membrane (3) and the hydrophilic membrane (6) form the injection channel (12); and the detection hole (53), the hydrophobic layer (4), the light-transmitting membrane (3) and the hydrophilic membrane (6) form the reaction detection area (13).

8. The photochemical analysis test strip structure according to claim 7, characterized in that: A super-hydrophilic guide belt (63) is provided on the bottom surface of the hydrophilic membrane (6), and the super-hydrophilic guide belt (63) covers the outlet of the injection hole (51) and the injection guide groove (52).

9. The photochemical analysis test strip structure according to claim 7, characterized in that: The test strip body (1) further comprises a cover film (2) arranged on the bottom surface of the light-transmitting film (3), and a light-transmitting hole (21) corresponding to the detection hole (53) is formed on the cover film (2).

10. The photochemical analysis test strip structure according to claim 1, characterized in that: The test strip body (1) is also provided with a waste liquid storage area (14), the waste liquid storage area (14) is connected to the reaction detection area (13) and is located downstream of the reaction detection area (13); the test strip body (1) is provided with a second air hole (64) connected to the reaction detection area (13).

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