Quality safety rapid detection method of whole grain food and detection instrument thereof

Abstract

The invention discloses a whole grain food quality safety rapid detection method and a detection instrument thereof.The detection instrument comprises a lower box body and an upper box body, an eight-channel colloidal gold detector and two storage boxes are installed in the lower box body, and test paper and a test solution bottle are installed in the two storage boxes respectively; a base is fixedly connected to the interior of the upper box body, and a preparation assembly is arranged in the base. The grinding disc is driven by the driving motor, cereal food is ground through the grinding disc, cereal food powder is prepared, then the cereal food powder is put into the other shell, a test solution in the test solution bottle is poured into the shell, an extracting solution is prepared, and then the content of mycotoxin can be detected at the moment; according to the invention, the tedious step of overall assembly is omitted, the detection operation can be carried out by opening the box body, the detection efficiency is improved, the condition of detection failure caused by misoperation is avoided, and the detection accuracy is improved.

Description

Technical Field

[0001] The present invention relates to a detection method and its detection instrument, specifically a rapid detection method and its detection instrument for the quality and safety of whole grain foods, belonging to the technical field of detection instruments. Background Technique

[0002] Whole grains are grains that retain the caryopsis structure after removing inedible parts such as the husk, maximizing the retention of natural nutrients and active substances in the grains. Whole grain foods include oats, brown rice, corn, sorghum, buckwheat, millet, barley, hulless barley, etc. These whole grain foods are not completely destroyed in the processing process, retaining the starchy endosperm, germ and cortex, and retaining rich nutrients such as protein, vitamins, carbohydrates and dietary fiber.

[0003] Mycotoxins are toxic secondary metabolites produced by molds during the growth process on foods such as grains. These toxins pose potential hazards to human health and may cause acute or chronic poisoning. Through detection, grains containing excessive mycotoxins can be discovered and excluded in a timely manner, thus ensuring the safety of grain products circulating in the market and protecting the health of consumers.

[0004] In the traditional technology for mycotoxin detection, sampling and preservation are required, and then the samples are taken to the laboratory for detection. The detection process is long, and it cannot quickly detect food safety, delaying the transportation and storage of grains, etc. Compared with the traditional method of detecting mycotoxins in the laboratory, the above detection device can perform rapid detection in various environments such as on-site, in warehouses, and in farmlands, greatly facilitating the detection operation and improving the detection efficiency. However, in the actual operation process, there are also some deficiencies: When performing the detection operation, each component needs to be taken out of the toolbox and assembled. This process is relatively cumbersome, including taking out components such as test strips, the main body of the eight-channel colloidal gold detector, and the preparation liquid tank, and correctly assembling and connecting them according to the instructions or operation manuals. This process not only requires a certain amount of time but may also lead to detection failure or inaccurate results due to improper operation or component damage. Therefore, a rapid detection method and its detection instrument for the quality and safety of whole grain foods are proposed. Summary of the Invention

[0005] The purpose of the present invention is to provide a rapid detection method and its detection instrument for the quality and safety of whole grain foods to solve one of the problems raised in the above background technique.

[0006] The present invention is implemented by the following technical solutions: First aspect: A rapid detection method for the quality and safety of whole grain foods, including the following steps: Step 1. Sample pretreatment: Put 20 g of cereal food sample into the grinding chamber, start variable-frequency grinding (gradient change from 50 Hz to 100 Hz), and after passing through an 80-mesh sieve, take 5.0 g of the powder and add 15 mL of acetonitrile-aqueous solution (ratio 84:16). After mixing with a vortex oscillator (rotation speed 2500 rpm, duration 1 min), let it stand for stratification; Step 2. Intelligent purification and extraction: The supernatant is purified through a pre-packed C18 column (500 mg / 6 mL), and the eluate is concentrated to 1 mL by nitrogen blowing and then transferred to the mixing chamber, and 5 mL of PBS buffer solution (pH 7.4, containing 0.1% Tween-20) is added; Step 3. Multi-target synchronous detection: The extract is vertically dropped onto the sample pad of the test strip (100 μL / well). After chromatography for 10 min, it is placed in an eight-channel colloidal gold detector, and multi-spectral scanning (405 / 520 / 650 nm) is started. The system automatically identifies the displacement of the AFB1 and ZEN detection lines (ΔL ± 0.01 mm); Step 4. Cloud big data analysis: The detection data is uploaded to the cloud platform through the NB-IoT module, and the deep residual network (ResNet-18) is called for image feature extraction. Combining the origin traceability information stored in the blockchain to correct the detection results, a PDF report (including a two-dimensional anti-counterfeiting code) is generated and pushed to the user terminal in real time.

[0007] The working principle or structural principle is such that the detection sensitivity is increased to 0.05 μg / kg (AFB1) and 0.1 μg / kg (ZEN), the single detection time is shortened to 15 minutes (the traditional method takes 45 minutes); the error rate of multi-toxin synchronous detection is <5% (the traditional test strip >15%); the recovery rate is increased to 92 - 105% through temperature-controlled extraction (the traditional method is 85 - 95%).

[0008] As a further optimization of this technical solution, the extract is vertically dropped onto the sample pad of the test strip, and after chromatography, it is placed in an eight-channel colloidal gold detector, and multi-spectral scanning is started. The eight-channel colloidal gold detector automatically identifies the displacement of the AFB1 and ZEN detection lines, specifically including: Integrate 405 nm / 520 nm / 650 nm three-wavelength LED light sources based on the eight-channel colloidal gold detector; Capture the chromatography process at a frame rate of 120 fps according to the CMOS image sensor; Real-time track the color development kinetic curve of the detection line through the improved YOLOv5 algorithm; Construct a time-chromaticity three-dimensional standard curve (R² > 0.99).

[0009] Second aspect: A rapid detection instrument for the quality and safety of whole grain foods, comprising a lower box body and an upper box body. An eight-channel colloidal gold detector and two storage boxes are installed inside the lower box body. Test strips and test solution bottles are respectively installed inside the two storage boxes. A base is fixedly connected inside the upper box body, and a preparation component is arranged inside the base; The preparation component includes two connecting seats, two connecting disks, two outer shells, a driving seat, a driving motor and two connecting shafts; A grinding disk and a stirring rod are respectively arranged inside the two outer shells. The driving seat is fixedly connected to the output shaft of the driving motor. Sealing covers are screwed to the tops of the two outer shells. One connecting seat and one connecting disk are symmetrically fixedly connected to the top and bottom ends of the grinding disk respectively, and the other connecting seat and the other connecting disk are symmetrically fixedly connected to the top and bottom ends of the stirring rod respectively. The two connecting shafts are rotatably connected inside the two outer shells, and a driving disk is fixedly connected to the top end of the connecting shaft.

[0010] As a further preference of this technical solution: The preparation component further includes four limiting blocks, an insertion post, insertion holes, a handle and a driving groove; The four limiting blocks are symmetrically fixedly connected to the outer side walls of the outer shells. The insertion post is symmetrically fixedly connected to the upper surface of the driving disk. The insertion holes are symmetrically opened inside the connecting disk. The driving groove is opened inside the connecting shaft. The handle is fixedly connected to the upper surface of the sealing cover.

[0011] As a further preference of this technical solution: The insertion post is inserted into the insertion hole. The connecting seat is rotatably connected inside the sealing cover. The driving disk is fixedly connected to the top end of the connecting shaft. The size of the driving groove is adapted to the size of the driving seat.

[0012] As a further preference of this technical solution: Three limiting grooves are equidistantly opened on the upper surface of the base. The two outer shells are symmetrically slidably connected to the inner side walls of the two limiting grooves. The driving motor is embedded in the inner bottom wall of the other limiting groove.

[0013] As a further preference of this technical solution: Four guiding guard plates are symmetrically fixedly connected to the upper surface of the base. The guiding guard plates are annularly distributed outside the driving motor. Two measuring tool grooves are opened on the upper surface of the base, and measuring cups are installed inside the measuring tool grooves.

[0014] As a further preference of this technical solution: A limiting component is installed inside the upper box body. The limiting component includes a limiting baffle, a limiting plate, a sliding groove, two pull rods, two limiting insertion rods, a spring and two guiding rods; The limiting baffle is rotatably connected to the top of the inner side wall of the upper box body. The limiting plate is fixedly connected to one side of the limiting baffle. The sliding groove is formed on the upper surface of the limiting plate. The two pull rods are symmetrically slidably connected to the inner side wall of the sliding groove. The two limiting insertion rods are symmetrically fixedly connected to the opposite surfaces of the two pull rods. The two pull rods are slidably connected to the outer side walls of the two guide rods. The spring is sleeved on the outer side wall of the guide rod.

[0015] As a further preference of this technical solution: The two ends of the spring abut against the adjacent surfaces of the two pull rods. The limiting insertion rod is slidably connected to the inside of the limiting plate. A tool strap is fixedly connected to the lower surface of the limiting baffle.

[0016] As a further preference of this technical solution: A groove is formed on one side of the upper surface of the upper box body. The limiting plate is slidably connected to the inside of the groove. A limiting hole is formed in the inside of the groove. The limiting insertion rod is inserted into the limiting hole.

[0017] As a further preference of this technical solution: A lock catch is installed on the front surface of the lower box body. Support feet are installed on the outer side walls of the upper box body and the lower box body.

[0018] Advantages of the present invention: 1. In the present invention, after weighing the cereal food sample, it is placed into the outer shell. The grinding disc is driven by the driving motor, and the cereal food is ground by the grinding disc to prepare cereal food powder. Then the cereal food powder is put into another outer shell, the test solution in the test solution bottle is poured into the outer shell, and under the drive of the driving motor, the stirring rod mixes the test solution with the powder. Through the high-speed agitation of the stirring rod, an extract can be prepared, and at this time, the detection of the mycotoxin content can be carried out; 2. The present invention eliminates the cumbersome overall assembly steps. The detection operation can be carried out by opening the box body, which improves the detection efficiency, avoids the situation of detection failure caused by improper operation, and improves the detection accuracy. Description of the drawings

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.

[0020] Figure 1 It is a flowchart of a method for rapid detection of the quality and safety of whole grain foods according to the present invention; Figure 2 It is a structural schematic diagram of a rapid detection instrument for the quality and safety of whole grain foods according to the present invention; Figure 3 Schematic diagram of the buckling structure of the lower box body and the upper box body in the second embodiment of the present invention; Figure 4 Schematic diagram of the opened limiting component in the second embodiment of the present invention; Figure 5 Schematic diagram of the preparation component structure in the second embodiment of the present invention; Figure 6 Exploded schematic diagram of the preparation component structure in the second embodiment of the present invention; Figure 7 Working schematic diagram of the preparation component in the second embodiment of the present invention; Figure 8 Schematic diagram of the grinding disc structure in the second embodiment of the present invention; Figure 9 Schematic diagram of the connecting shaft structure in the second embodiment of the present invention; Figure 10 Schematic diagram of the stirring rod structure in the second embodiment of the present invention; Figure 11 Schematic diagram of the limiting plate structure in the second embodiment of the present invention; Figure 12 Schematic diagram of the connection between the pull rod and the limiting insertion rod in the second embodiment of the present invention.

[0021] In the figure: 11, lower box body; 12, upper box body; 13, support feet; 14, lock; 16, eight-channel colloidal gold detector; 17, test strip; 18, storage box; 19, test solution bottle; 20, base; 301, preparation component; 31, sealing cover; 32, connecting seat; 33, grinding disc; 34, connecting disc; 35, driving disc; 36, outer shell; 37, driving seat; 38, driving motor; 39, connecting shaft; 40, limiting block; 41, inserting column; 42, inserting hole; 43, handle; 44, driving groove; 45, stirring rod; 46, limiting groove; 47, measuring tool groove; 48, guiding guard plate; 49, measuring cup; 501, limiting component; 51, limiting baffle; 52, tool binding strap; 53, limiting plate; 54, sliding groove; 55, pull rod; 56, limiting insertion rod; 57, spring; 58, guiding rod; 59, groove. Detailed implementation manners

[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0023] Embodiment 1 For this purpose, please refer to Figure 1, the present invention provides a technical solution: a rapid method for detecting the quality and safety of whole grain foods, comprising the following steps: Step 1, sample pretreatment: Put 20 g of the grain food sample into the grinding chamber, start variable-frequency grinding (gradient change from 50 Hz to 100 Hz), and after passing through an 80-mesh sieve, take 5.0 g of the powder and add 15 mL of acetonitrile-aqueous solution (ratio 84:16), mix by a vortex oscillator (rotation speed 2500 rpm, duration 1 min), and then let it stand for layering; Specifically, weigh 20 g of the whole grain food sample to be detected to ensure that the sample is uniform and meets the experimental requirements. Use a high-precision electronic balance (for example, with an accuracy of 0.001 g) for weighing to avoid the influence of weighing errors on subsequent analysis; put the sample into the variable-frequency grinding chamber, start the variable-frequency grinding device and set the grinding frequency to change in a gradient from 50 Hz to 100 Hz, perform efficient grinding in a short time to avoid overheating or uneven grinding of the sample. During the grinding process, the equipment automatically monitors the grinding effect and ensures that the particle fineness reaches the ideal level through vibration monitoring. Screen the ground sample through an 80-mesh sieve to remove larger particles and ensure uniform powder. Use appropriate screening equipment to ensure that the sieved powder does not exceed 5.0 g. Add 5.0 g of the sieved powder to 15 mL of acetonitrile-aqueous solution (ratio: acetonitrile 84%, water 16%). Acetonitrile is a commonly used polar solvent, which helps to extract mycotoxins from the grain powder. After adding the solvent, use a vortex oscillator to mix. Set the rotation speed of the vortex oscillator to 2500 rpm and continuously oscillate for 1 minute to ensure uniform mixing of the sample and the solvent. After mixing, let the sample solution stand for layering. The upper layer of the solution is the clear liquid containing the dissolved toxin, and the lower layer is the undissolved impurities. The standing process is generally 10 - 15 minutes until obvious layering occurs to ensure a clear supernatant is obtained.

[0024] Step 2, intelligent purification and extraction: The supernatant is purified through a pre-packed C18 column (500 mg / 6 mL), and the eluate is concentrated to 1 mL by nitrogen blowing and then transferred to the mixing chamber, and 5 mL of PBS buffer solution (pH 7.4, containing 0.1% Tween-20) is added; Specifically, a C18 solid phase extraction column (500 mg / 6 mL) was used. The C18 column is a commonly used reverse phase extraction material that can effectively remove impurities and other interfering substances in the sample. First, the solid phase extraction column was activated with 5 mL of methanol, and then rinsed with 5 mL of water to ensure that the inside of the extraction column was clean and suitable for sample extraction. The supernatant after stratification was slowly passed through the C18 solid phase extraction column so that the mycotoxins in it were adsorbed by the column, and the toxins were eluted through an appropriate solvent (acetonitrile-water solution 84:16, v / v). The purpose of this process is to elute the toxins from the solid phase extraction column into the solution. After solid phase extraction through the C18 column, the target mycotoxin is eluted with acetonitrile-water solution (84:16, v / v). The elution solvent is a mixture of acetonitrile and water. Such a solvent combination can effectively separate the target toxins. After collection, it is ensured that there is no significant color difference in the liquid after each elution to ensure purity. The eluate is concentrated to 1 mL using a nitrogen blow concentration module. Set the nitrogen blowing module to work at 50°C and 0.2MPa pressure to quickly remove excess solvent and concentrate the target toxin in a short time (about 5 minutes). Nitrogen blowing concentration helps to increase the toxin concentration in the extract and ensure the sensitivity of subsequent detection. Add 5mL PBS buffer (pH 7.4, containing 0.1% Tween-20) to the concentrated extract. PBS buffer can help stabilize the pH value of the extract and reduce possible errors during sample processing. Tween-20 is a non-ionic surfactant that helps improve the solubility of toxins in the extract and ensure uniform dispersion. Use a mixer or vortex oscillator to fully mix the solution to ensure that the PBS buffer is completely integrated with the extract. Set the mixing speed to 2500rpm and the mixing time to 1-2 minutes until the solution is completely uniform.

[0025] Step 3, multi-target simultaneous detection: the extract was vertically dripped onto the sample pad of the test strip (100 μL / well), and after chromatography for 10 min, it was placed into an eight-channel colloidal gold detector, and multi-spectral scanning (405 / 520 / 650 nm) was started. The system automatically identified the displacement of the AFB1 and ZEN detection lines (ΔL±0.01 mm); Specifically include: Based on eight-channel colloidal gold detector, integrated with 405nm / 520nm / 650nm three-wavelength LED light source; Capture the tomography process at 120fps based on CMOS image sensors; The improved YOLOv5 algorithm is used to track the color kinetic curve of the detection line in real time; Construct a time-chromaticity three-dimensional standard curve (R²>0.99); Specifically, insert the test strip into an eight-channel colloidal gold detector. This device is equipped with an automatic feeding system that can accurately align the test strip with the optical reading area of the eight-channel colloidal gold detector; During the reading process, the eight-channel colloidal gold detector automatically irradiates the test strip with multi-angle light through the built-in three-wavelength LED light source (405nm, 520nm, 650nm) to stimulate the antibody reaction products labeled with colloidal gold; The eight-channel colloidal gold detector is built with a CMOS image sensor that captures images at a frame rate of 120fps. The sensor records the changes in light intensity in real time, especially focusing on the color development changes in the sample area and the detection line area; During the detection process, the eight-channel colloidal gold detector monitors the color development kinetic curve of the detection line based on real-time data. It uses the built-in algorithm to analyze the color intensity and position changes in real time; The system dynamically analyzes the color intensity through light sources of multiple wavelengths (405nm, 520nm, 650nm). The response of each wavelength helps to distinguish the reaction characteristics of different types of mycotoxins (such as AFB1 and ZEN); The system in the eight-channel colloidal gold detector automatically records the color intensity of each channel, makes a comprehensive judgment to ensure the accuracy of the results; The system presets a set of standard curves (based on multiple samples and different concentrations of mycotoxins), and the concentration range of each toxin (such as aflatoxin B1 and zearalenone) varies from 0.05 μg / kg to 100 μg / kg; The system compares the color intensity of the detection line with the standard curve to calculate the concentration of mycotoxins in the sample; Using the relationship between the color development intensity and the mycotoxin concentration, based on the slope of the color change and the fitting degree of the standard curve, the system calculates the concentration of the target toxin according to the algorithm; This process is completed by calculating the intensity changes of each detection channel and mapping them to the corresponding toxin concentration range; To improve the detection accuracy, the system is built with multiple data correction algorithms, such as matrix effect correction, temperature influence correction, etc. The detection results will be displayed as the final mycotoxin concentration after correction; The eight-channel colloidal gold detector automatically displays the calculated mycotoxin concentration results on the display screen for the user. At the same time, the system generates a detailed detection report, recording the type of toxin measured, the concentration value, and the test conditions; Step 4, Cloud big data analysis: The detection data is uploaded to the cloud platform through the NB-IoT module, and the deep residual network (ResNet-18) is called for image feature extraction. The detection results are corrected by combining the origin traceability information stored in the blockchain, and a PDF report (including a two-dimensional anti-counterfeiting code) is generated and pushed to the user terminal in real time.

[0026] Specifically, the results will be uploaded to the cloud platform in real time for storage and further analysis. The cloud system uses big data technology for cross-verification, data analysis, and trend recognition to make real-time evaluations of different batches of samples; On the cloud platform, based on the uploaded detection data, the system uses a deep residual network (ResNet-18) to extract image features, further improving the accuracy and robustness of the data; Combined with the origin information of the product (through blockchain traceability), the system can further optimize the detection results to ensure the authenticity and reliability of the detection results; the cloud platform will generate a real-time PDF detection report according to the analysis results. The report includes sample information, mycotoxin concentration, detection methods, and quality control information. The report will also include a QR code anti-counterfeiting function to ensure the authenticity and immutability of the report.

[0027] This report will be pushed to the user terminal in real time through the notification system, and users can view it at any time.

[0028] Specifically, dynamic multispectral scanning is performed through LED light sources with three wavelengths of 405nm, 520nm, and 650nm, improving the recognition accuracy of the samples.

[0029] The improved YOLOv5 algorithm is used to track the color development curve in real time to ensure that every detail in the detection process is precisely controlled.

[0030] The combination of the built-in standard curve and real-time chromaticity analysis ensures the accurate calculation of the sample concentration, avoiding errors caused by manual intervention.

[0031] The influence of the environment and sample processing on the detection results is eliminated through multiple correction algorithms (such as temperature, matrix, etc.), improving the detection accuracy.

[0032] With the help of the cloud deep learning model and blockchain technology, the intelligent level of data analysis is improved, and the security and traceability of the data are ensured.

[0033] Embodiment 2 In the prior art, during the detection operation, it is necessary to take out and assemble each component from the toolbox. This process is relatively cumbersome, which includes taking out components such as test strips, the main body of the eight-channel colloidal gold detector, batteries, connecting wires, etc., and correctly assembling and connecting them according to the instruction manual or operation guide. This process not only requires a certain amount of time but may also lead to detection failure or inaccurate results due to improper operation or component damage; To this end, please refer to Figures 2 - 12, the present invention provides a technical solution: a rapid detection instrument for the quality and safety of whole grain foods, including a lower box body 11 and an upper box body 12. An eight-channel colloidal gold detector 16 and two storage boxes 18 are installed inside the lower box body 11. Test strips 17 and test solution bottles 19 are respectively installed inside the two storage boxes 18. A base 20 is fixedly connected inside the upper box body 12. The main body of the detection instrument is installed inside the box body, which is convenient for carrying the whole; A preparation component 301 is arranged inside the base 20. The preparation component 301 is used for preparing the extract of grain foods, including steps such as grinding and mixing. After the extract is prepared, the extract is dropped onto the sample application area of the test strip 17. The mycotoxin in the sample binds to the colloidal gold-labeled antibody on the test strip 17. This binding inhibits the binding of the antibody and the toxin-conjugate on the detection line. The color intensity on the detection line will change. This change is closely related to the content of the toxin in the sample. Then the test strip 17 is inserted into the eight-channel colloidal gold detector 16. The eight-channel colloidal gold detector 16 automatically reads the color intensity on the detection line and calculates the content of the mycotoxin in the sample according to the built-in standard curve, thereby realizing the detection of the mycotoxin content; The preparation component 301 includes two connecting seats 32, two connecting disks 34, two outer shells 36, a driving seat 37, a driving motor 38 and two connecting shafts 39; A grinding disk 33 and a stirring rod 45 are respectively arranged inside the two outer shells 36, so that grinding and mixing operations can be respectively carried out inside the two outer shells 36. Sealing covers 31 are threadedly connected to the tops of the two outer shells 36. The sealing covers 31 can ensure the sealing performance during the preparation process. One connecting seat 32 and one connecting disk 34 are symmetrically fixedly connected to the top and bottom ends of the grinding disk 33, and the other connecting seat 32 and the other connecting disk 34 are symmetrically fixedly connected to the top and bottom ends of the stirring rod 45. The two connecting shafts 39 are rotatably connected inside the two outer shells 36. A driving disk 35 is fixedly connected to the top end of the connecting shaft 39. Power can be transmitted through the connecting shaft 39, and the positions of the grinding disk 33 and the stirring rod 45 can be limited through the connecting seat 32; The driving seat 37 is fixedly connected to the output shaft of the driving motor 38, and the size of the driving groove 44 is adapted to the size of the driving seat 37; When grinding grain foods, put the grain food sample into the outer shell 36, then take out the outer shell 36, dock the driving seat 37 with the driving groove 44, drive the driving seat 37 through the driving motor 38, the driving seat 37 drives the connecting shaft 39 to rotate, the connecting shaft 39 drives the connecting disk 34 through the driving disk 35, and the connecting disk 34 drives the grinding disk 33 to rotate, so that the grain food can be ground by the grinding disk 33 to prepare the grain food powder; When preparing the extraction solution, the prepared cereal food powder is put into another outer shell 36, and then the test solution in the test solution bottle 19 is poured into the outer shell 36. Driven by the drive motor 38, the stirring rod 45 mixes the test solution with the powder. Through the high-speed agitation of the stirring rod 45, the extraction solution can be prepared, and at this time, the detection of the mycotoxin content can be carried out; In this embodiment, specifically: the preparation assembly 301 further includes four limit blocks 40, insertion posts 41, insertion holes 42, a handle 43 and a drive groove 44; The insertion posts 41 are symmetrically and fixedly connected to the upper surface of the drive disk 35, the insertion holes 42 are symmetrically opened inside the connection disk 34, the drive groove 44 is opened inside the connection shaft 39, the handle 43 is fixedly connected to the upper surface of the sealing cover 31, the insertion posts 41 are inserted into the insertion holes 42, the drive disk 35 is fixedly connected to the top end of the connection shaft 39. Through the docking of the drive groove 44 and the drive seat 37, the power can be transmitted into the connection shaft 39. When the connection shaft 39 rotates, it drives the drive disk 35. The drive disk 35 drives the connection disk 34 through the insertion posts 41, and the connection disk 34 drives the stirring rod 45 or the grinding disk 33, so as to realize the preparation of the extraction solution; The connection seat 32 is rotatably connected inside the sealing cover 31. Through the connection seat 32, the positions of the stirring rod 45 and the grinding disk 33 can be limited. When the stirring rod 45 and the grinding disk 33 rotate at high speed, there will be no eccentric shaking. Moreover, since the sealing cover 31 is threadedly connected to the outer shell 36, when the outer shell 36 is removed, the stirring rod 45 and the grinding disk 33 can be taken out together for cleaning.

[0034] In this embodiment, specifically: three limit grooves 46 are equidistantly opened on the upper surface of the base 20. Two outer shells 36 are symmetrically and slidably connected to the inner side walls of the two limit grooves 46, and the drive motor 38 is embedded in the inner bottom wall of another limit groove 46. Through the limit grooves 46, the positions of the outer shells 36 can be limited.

[0035] In this embodiment, specifically: four guiding guard plates 48 are symmetrically and fixedly connected to the upper surface of the base 20. The guiding guard plates 48 are annularly distributed outside the drive motor 38. Four limit blocks 40 are symmetrically and fixedly connected to the outer side wall of the outer shell 36. Through the guiding guard plates 48, the outer shell 36 can be limited, and during the preparation process, the outer shell 36 can be prevented from tipping over. Through the limit blocks 40, the outer shell 36 can be prevented from rotating axially; Two measuring tool grooves 47 are opened on the upper surface of the base 20. Measuring cups 49 are installed inside the measuring tool grooves 47. During the preparation process, the dosages of the cereal food powder and the test solution can be measured respectively through the two measuring cups 49.

[0036] In order to solve the problems existing in the prior art, the embodiment of the present invention provides a rapid quality and safety detection instrument for whole grain food, and solves the problems through the above technical solutions: The weighed cereal food sample is put into the shell 36, the shell 36 is taken out from the limiting groove 46, the driving seat 37 is docked with the driving groove 44, the driving motor 38 is controlled to work, the driving motor 38 drives the driving seat 37, the driving seat 37 drives the connecting shaft 39 to rotate, the connecting shaft 39 drives the connecting disk 34 through the driving disk 35, the connecting disk 34 drives the grinding disk 33, the cereal food is ground by the grinding disk 33 to prepare the cereal food powder, and then the cereal food powder is put into another shell 36, the test solution in the test solution bottle 19 is poured into the shell 36, and under the drive of the driving motor 38, the stirring rod 45 mixes the test solution with the powder, and the extract can be prepared through the high-speed stirring of the stirring rod 45, and then the mycotoxin content can be detected at this time. Compared with the prior art, the present invention eliminates the cumbersome overall assembly steps, and the detection operation can be carried out by opening the box, thereby improving the detection efficiency, avoiding the detection failure caused by improper operation, and improving the accuracy of the detection.

[0037] A filtering system can also be provided on the lower box 11 according to usage requirements. The filter is installed on a bracket. A platform for placing the test paper 17 is designed at the bottom of the bracket. The test solution is poured into the filter after being fully stirred and mixed. The filter uses the filter medium inside it to remove solid particles and impurities in the test solution, thereby ensuring the purity of the test solution. After filtration, an extract is obtained, and the extract flows out through the outlet pipe of the filter. A solenoid valve and a flow sensor are provided on the outlet pipe. By controlling the opening and closing of the solenoid valve, the flow of the test solution can be accurately regulated.

[0038] In this embodiment, specifically: a limiting assembly 501 is installed inside the upper box 12, and the limiting assembly 501 is used to limit the position of the preparation assembly 301. When the lower box 11 is buckled with the upper box 12 and carried, the limiting assembly 501 respectively supports the handle 43, thereby preventing the housing 36 from falling from the limiting groove 46; The limiting assembly 501 includes a limiting baffle 51, a limiting plate 53, a slide slot 54, two pull rods 55, two limiting plug rods 56, a spring 57 and two guide rods 58; The limit baffle 51 is rotatably connected to the top of the inner wall of the upper box body 12. The limit baffle 51 is a hollow structure, which ensures the limit effect while reducing the mass; The limit plate 53 is fixedly connected to one side of the limit baffle 51. A chute 54 is formed on the upper surface of the limit plate 53. Two pull rods 55 are symmetrically and slidably connected to the inner side wall of the chute 54. The position of the pull rods 55 can be limited through the chute 54. Two limit insertion rods 56 are symmetrically and fixedly connected to the opposite surfaces of the two pull rods 55. The two pull rods 55 are slidably connected to the outer side walls of two guide rods 58. A spring 57 is sleeved on the outer side walls of the guide rods 58. Two ends of the spring 57 abut against the adjacent surfaces of the two pull rods 55. The limit insertion rods 56 are slidably connected to the inside of the limit plate 53. By pulling the two pull rods 55, the two pull rods 55 approach each other. At this time, the spring 57 is compressed under force. The pull rods 55 drive the limit insertion rods 56, and thus the unlocking action can be realized. When the pull rods 55 are released, the spring 57 pushes the pull rods 55, and the pull rods 55 drive the limit insertion rods 56 to insert into the inside of the upper box body 12, and thus the locking action is realized, and the position of the preparation assembly 301 can be limited; A tool strap 52 is fixedly connected to the lower surface of the limit baffle 51. Tools such as pliers, tweezers, test tubes, etc. can be placed in the tool strap 52 according to actual usage requirements.

[0039] In this embodiment, specifically: a groove 59 is formed on one side of the upper surface of the upper box body 12. The limit plate 53 is slidably connected to the inside of the groove 59. A limit hole is formed in the inside of the groove 59. The limit insertion rod 56 is inserted into the limit hole. When the limit insertion rod 56 is inserted into the inside of the limit hole, the position of the limit baffle 51 is fixed.

[0040] In this embodiment, specifically: a lock catch 14 is installed on the front surface of the lower box body 11. The upper box body 12 and the lower box body 11 can be connected through the lock catch 14, which is convenient for carrying. Support feet 13 are installed on the outer side walls of the upper box body 12 and the lower box body 11. When the upper box body 12 is opened, the overall stability can be enhanced through the support feet 13.

[0041] During the working principle or structural principle, in use, open the upper box body 12. Both the upper box body 12 and the lower box body 11 are in contact with the ground through the support feet 13. Then pull the two pull rods 55. The two pull rods 55 approach each other. At this time, the spring 57 is compressed under force. The pull rods 55 drive the limit insertion rods 56, and the limit insertion rods 56 are disengaged from the limit holes. Then rotate the limit baffle 51 to turn over the limit baffle 51 to leave a working space for the preparation assembly 301; Put the weighed cereal food sample into the outer shell 36. After covering the sealing cover 31, take out the outer shell 36 from the limiting groove 46, dock the driving seat 37 with the driving groove 44, control the driving motor 38 to work, the driving motor 38 drives the driving seat 37, the driving seat 37 drives the connecting shaft 39 to rotate, the connecting shaft 39 drives the connecting disk 34 through the driving disk 35, the connecting disk 34 drives the grinding disk 33, and grind the cereal food through the grinding disk 33 to prepare cereal food powder. Then open the outer shell 36, put the cereal food powder into another outer shell 36, pour the test solution in the test solution bottle 19 into the outer shell 36 and cover the sealing cover 31. Driven by the driving motor 38, the stirring rod 45 mixes the test solution and the powder. Through the high-speed agitation of the stirring rod 45, the extract can be prepared. Drop the extract onto the sample addition area of the test strip 17. The mycotoxin in the sample binds to the colloidal gold-labeled antibody on the test strip 17, and the color depth on the detection line changes. Then insert the test strip 17 into the eight-channel colloidal gold detector 16. The eight-channel colloidal gold detector 16 automatically reads the color intensity on the detection line and calculates the content of mycotoxin in the sample according to the built-in standard curve, thereby realizing the detection of the mycotoxin content.

[0042] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A method for rapid detection of the quality and safety of whole grain food, characterized in that: The following steps are involved: Step 1, sample pretreatment: put the cereal food sample into the grinding chamber, start the variable frequency grinding, take the powder after sieving, add acetonitrile-water solution, mix it with a vortex oscillator, and then stand for stratification; Step 2, intelligent purification and extraction: the supernatant is purified by a pre-loaded C18 column, the eluate is concentrated by nitrogen blowing and transferred to a mixing chamber, and PBS buffer is added; Step 3, multi-target simultaneous detection: the extract is dripped vertically onto the sample pad of the test strip, and after chromatography, it is placed into an eight-channel colloidal gold detector, and multi-spectral scanning is started. The eight-channel colloidal gold detector automatically identifies the displacement of the AFB1 and ZEN detection lines; Step 4: Cloud-based big data analysis: The test data is uploaded to the cloud platform through the NB-IoT module, and the deep residual network is called to extract image features. The test results are corrected in combination with the origin traceability information stored in the blockchain, and a PDF report is generated and pushed to the user terminal in real time.

2. A method for rapid quality and safety detection of whole grain food according to claim 1, characterized in that: The extract is dripped vertically onto the test strip sample pad, and after chromatography, it is placed into an eight-channel colloidal gold detector, and multi-spectral scanning is started. The eight-channel colloidal gold detector automatically identifies the displacement of the AFB1 and ZEN detection lines, specifically including: Based on eight-channel colloidal gold detector, integrated with 405nm / 520nm / 650nm three-wavelength LED light source; Capture the tomography process at 120fps based on CMOS image sensors; The improved YOLOv5 algorithm is used to track the color kinetic curve of the detection line in real time; Construct a three-dimensional time-chromaticity standard curve.

3. A whole grain food quality and safety rapid detection instrument, applied to a whole grain food quality and safety rapid detection method as claimed in claim 1 or 2, characterized in that: The device comprises a lower box (11) and an upper box (12); an eight-channel colloidal gold detector (16) and two storage boxes (18) are installed inside the lower box (11); test papers (17) and test solution bottles (19) are installed inside the two storage boxes (18), respectively; a base (20) is fixedly connected inside the upper box (12); a preparation component (301) is arranged inside the base (20); The preparation assembly (301) comprises two connection seats (32), two connection plates (34), two housings (36), a drive seat (37), a drive motor (38) and two connection shafts (39); A grinding disc (33) and a stirring rod (45) are respectively arranged inside the two outer shells (36); the driving seat (37) is fixedly connected to the output shaft of the driving motor (38); the tops of the two outer shells (36) are threadedly connected to sealing covers (31); one connecting seat (32) and a connecting plate (34) are symmetrically fixedly connected to the top and bottom ends of the grinding disc (33); the other connecting seat (32) and the other connecting plate (34) are symmetrically fixedly connected to the top and bottom ends of the stirring rod (45); the two connecting shafts (39) are rotatably connected to the inside of the two outer shells (36); and the top ends of the connecting shafts (39) are fixedly connected to the driving disc (35).

4. The whole grain food quality and safety rapid testing instrument according to claim 3, characterized in that: The preparation assembly (301) further comprises four limit blocks (40), a plug post (41), a plug hole (42), a handle (43) and a drive slot (44); The four limit blocks (40) are symmetrically fixedly connected to the outer wall of the housing (36), the plug posts (41) are symmetrically fixedly connected to the upper surface of the drive disk (35), the plug holes (42) are symmetrically opened inside the connecting disk (34), the driving grooves (44) are opened inside the connecting shaft (39), and the handle (43) is fixedly connected to the upper surface of the sealing cover (31).

5. The whole grain food quality and safety rapid testing instrument according to claim 4, characterized in that: The plug post (41) is inserted into the interior of the plug hole (42), the connecting seat (32) is rotatably connected to the interior of the sealing cover (31), the driving disk (35) is fixedly connected to the top end of the connecting shaft (39), and the size of the driving groove (44) is compatible with the size of the driving seat (37).

6. The whole grain food quality and safety rapid testing instrument according to claim 4, characterized in that: The upper surface of the base (20) is provided with three limit slots (46) at equal intervals, the two housings (36) are symmetrically slidably connected to the inner side walls of the two limit slots (46), and the drive motor (38) is embedded in the inner bottom wall of another limit slot (46).

7. The whole grain food quality and safety rapid testing instrument according to claim 6, characterized in that: Four guide guard plates (48) are symmetrically fixedly connected to the upper surface of the base (20), and the guide guard plates (48) are distributed in an annular manner outside the drive motor (38). Two measuring tool grooves (47) are provided on the upper surface of the base (20), and measuring cups (49) are installed inside the measuring tool grooves (47).

8. The whole grain food quality and safety rapid testing instrument according to claim 3, characterized in that: A limit assembly (501) is installed inside the upper box body (12), and the limit assembly (501) comprises a limit baffle (51), a limit plate (53), a slide groove (54), two pull rods (55), two limit plug rods (56), a spring (57) and two guide rods (58); The limit baffle (51) is rotatably connected to the top of the inner wall of the upper box body (12), the limit plate (53) is fixedly connected to one side of the limit baffle (51), the slide groove (54) is opened on the upper surface of the limit plate (53), the two pull rods (55) are symmetrically slidably connected to the inner wall of the slide groove (54), the two limit plug rods (56) are symmetrically fixedly connected to the away surfaces of the two pull rods (55), the two pull rods (55) are slidably connected to the outer walls of the two guide rods (58), and the spring (57) is sleeved on the outer wall of the guide rod (58).

9. The whole grain food quality and safety rapid testing instrument according to claim 8, characterized in that: The two ends of the spring (57) support the adjacent surfaces of the two pull rods (55), the limit rod (56) is slidably connected to the inside of the limit plate (53), and the lower surface of the limit baffle (51) is fixedly connected to the tool strap (52); a groove (59) is provided on one side of the upper surface of the upper box body (12), the limit plate (53) is slidably connected to the inside of the groove (59), a limit hole is provided inside the groove (59), and the limit rod (56) is inserted into the limit hole.

10. The whole grain food quality and safety rapid testing instrument according to claim 3, characterized in that: A lock buckle (14) is installed on the front surface of the lower box body (11), and supporting feet (13) are installed on the outer side walls of the upper box body (12) and the lower box body (11).

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