Guanine glycoside-based bactericidal composition and application thereof

Abstract

The invention discloses a bactericidal composition based on guanine glycoside and application thereof. The bactericidal composition based on guanine glycoside comprises the following active ingredients: guanine glycoside, and at least one of boscalid, kasugamycin and ningnanmycin, the application comprises at least one of the following aspects: prevention and treatment of plant fungal diseases, prevention and treatment of plant bacterial diseases and prevention and treatment of plant virus diseases. The guanine glycoside is reasonably mixed with boscalid, kasugamycin and ningnanmycin, different pesticide formulations with remarkable synergistic effects are prepared through a conventional method, and the bactericidal composition can be used for preventing and treating various diseases such as fungi, bacteria and viruses of plants, is remarkable in synergistic effect, can reduce the pesticide dosage, saves the cost, and has a broad application prospect. The application range of the medicine is expanded, the safety is extremely high, and the environment-friendly effect is realized.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition based on guanoside and its application. Background Art

[0002] Pesticides are an important means for preventing and controlling crop diseases and increasing production and improving quality. However, the overuse and abuse of pesticides often cause non-point source pollution, excessive residues, etc., which have an adverse impact on the agricultural ecological environment. Implementing the action of reducing pesticide use and increasing efficiency is of great significance for improving the agricultural production environment, ensuring the quality and safety of agricultural products, and promoting the sustainable development of agriculture. Under the new form of ecological agriculture, it is urgent to seek more environmentally friendly, economical and efficient new plant protection methods.

[0003] With the increasing promotion of the development of green agriculture and food safety, biological pesticides, as a new type of agricultural agent that is more environmentally friendly, show great advantages. Biological pesticides are derived from nature and used in nature. They are easily degraded in nature, have no pesticide residues, are safe for the environment, safe for non-target organisms, and will not harm human health.

[0004] 2'-Deoxyguanosine riboside is an antiviral component isolated from a strain with extremely high antiviral activity. After being approved by the National Technical Committee for Pesticide Standardization, its Chinese common name is officially named "guanoside" (Letter on Naming the Chinese Common Name for "Guanoside", (2022) Nongbiaozi No. 077). Previous studies have found that guanoside can directly activate the ethylene and salicylic acid signal pathways and the PTI response. It can significantly stimulate the classical immune response of plants at 10 ng / ml. At the same time, it has a plant growth regulation effect, can promote crop growth, improve yield and quality, and has important value in the research and development of new biological pesticides in the future. Chinese patent document CN114600897A (application number: 202210496024.5) provides a preparation method and application of 2'-deoxyguanosine, guanosine and their compositions. This patent document discloses the preparation methods of 2'-deoxyguanosine, guanosine and their compositions and their applications in the antiviral direction, but does not conduct in-depth research and exploration on the synergistic effect of the compounding of guanoside with different chemical agents. Summary of the Invention

[0005] In view of the deficiencies of the prior art, the present invention provides a bactericidal composition based on guanoside and its application. The combination of guanoside and other pesticides can reduce the dosage of chemical pesticides, delay the drug resistance of crops, expand the functions of pesticides, is safe and friendly to crops and the environment, and has remarkable effects in preventing and controlling crop bacterial, fungal and viral diseases.

[0006] The technical solution adopted by the present invention to solve its technical problems is:

[0007] On the one hand, the present invention provides a bactericidal composition based on guanosine glycoside, the active ingredients of which include guanosine glycoside and at least one of boscalid, kasugamycin, and ningnanmycin.

[0008] Boscalid is a new type of nicotinamide fungicide developed by BASF in Germany. It has a broad bactericidal spectrum and is active against almost all types of fungal diseases. Boscalid belongs to the inhibitor of succinate coenzyme Q reductase in the mitochondrial respiratory chain, has a strong inhibitory ability on spore germination, and has no cross-resistance with other fungicides. Boscalid is very effective in controlling powdery mildew, gray mold, sclerotinia, and various rot diseases, and is also effective against resistant bacteria of other agents. It is mainly used for the control of diseases in rapeseed, grapes, fruit trees, vegetables, and field crops, etc.

[0009] Kasugamycin is an antibiotic product. Its mechanism of action is to inhibit the protein synthesis of pathogenic bacteria, and it has the characteristics of low residue and pollution-free. It is an environment-friendly green biological pesticide. Kasugamycin can be used to control various diseases caused by bacteria and some fungi, and is particularly effective against rice blast. With the recognition of its control effect, its application scope has gradually expanded.

[0010] Ningnanmycin is an innovative biological pesticide developed by the Chengdu Institute of Biology, Chinese Academy of Sciences. It can control plant virus diseases by inhibiting the replication of viral nucleic acids and the synthesis of coat proteins. It has been registered for tobacco mosaic virus disease, tomato virus disease, pepper virus disease, rice damping-off, soybean root rot, rice stripe disease, apple blotch leaf spot, and cucumber powdery mildew. In addition, it has been widely applied on a large scale in the control of sclerotinia of rapeseed, litchi downy blight, virus diseases of other crops, stem rot, gummy stem blight, powdery mildew and other diseases.

[0011] On the other hand, the present invention also provides the application of the bactericidal composition based on guanosine glycoside, and the application includes at least one of the following aspects:

[0012] a) Controlling plant fungal diseases;

[0013] b) Controlling plant bacterial diseases;

[0014] c) Controlling plant virus diseases.

[0015] In the embodiment of the present invention, a bactericidal composition for controlling plant fungal diseases is provided, the active ingredients of which are guanosine glycoside and boscalid, and the plant fungal disease is tomato gray mold.

[0016] Preferably according to the present invention, the mass ratio of guanosine glycoside to boscalid is 1:10 to 20:1.

[0017] More preferably, the mass ratio of guanosine glycoside to boscalid is 1:5 to 10:1.

[0018] Further preferably, the mass ratio of guanosine glycoside to boscalid is 1:2.

[0019] In the embodiment of the present invention, a bactericidal composition for preventing and controlling plant bacterial diseases is provided, and the active ingredients are guanosine glycoside and kasugamycin, and the plant bacterial disease is bacterial leaf spot.

[0020] Preferably, the mass ratio of guanosine glycoside to kasugamycin is 1:15 to 10:1.

[0021] Further preferably, the mass ratio of guanosine glycoside to kasugamycin is 5:1.

[0022] In the embodiment of the present invention, a composition for preventing and controlling plant viral diseases is provided, and the active ingredients are guanosine glycoside and ningnanmycin, and the plant viral disease is tobacco mosaic virus.

[0023] Preferably, the mass ratio of guanosine glycoside to ningnanmycin is 1:25 to 10:1.

[0024] Further preferably, the mass ratio of guanosine glycoside to ningnanmycin is 1:25 to 1:5.

[0025] Further preferably, the mass ratio of guanosine glycoside to ningnanmycin is 1:5.

[0026] The bactericidal composition provided by the present invention further includes auxiliaries, and the auxiliaries include dispersants, antifreezing agents, thickeners, defoaming agents, solvents, etc. or other known substances beneficial to the stability of the active ingredients in the preparation and the exertion of drug efficacy, and various auxiliary components commonly used or permitted to be used in the field of pesticide preparations are adopted.

[0027] The dosage forms of the bactericidal composition of the present invention include suspension concentrates, water dispersible granules, and wettable powders, and 6 kinds of pesticide mixtures such as 30% guanosine glycoside·boscalid suspension concentrate, 30% guanosine glycoside·boscalid water dispersible granules, 24% guanosine glycoside·kasugamycin wettable powder, 24% guanosine glycoside·kasugamycin water dispersible granules, 18% guanosine glycoside·ningnanmycin suspension concentrate, and 18% guanosine glycoside·ningnanmycin wettable powder are prepared. The pesticide dosage forms of the present invention include but are not limited to the above 6 kinds.

[0028] The beneficial effects of the present invention are as follows:

[0029] 1. In the present invention, guanosine glycoside is rationally mixed with boscalid, kasugamycin, and ningnanmycin, and different pesticide dosage forms with significant synergistic effects are prepared by conventional methods, which can be used to prevent and control various diseases such as plant fungi, bacteria, and viruses. The synergistic effect is significant, achieving the effect of 1+1>2; the dosage of pesticides can be reduced and the cost can be saved.

[0030] 2. The pesticide mixture of the present invention plays an important role in controlling plant fungal diseases (such as gray mold), bacterial diseases (such as bacterial leaf spot), and viral diseases (such as PVX). The control effect is significantly higher than that of single agents, and it has high safety, is friendly to the environment, and has great economic and social benefits.

[0031] 3. By reasonably combining and proportioning biological pesticides and conventional chemical pesticides, the present invention not only makes up for the disadvantages of single action and sharp reduction in resistance of chemical pesticides, reduces the amount of pesticide used, but also combines the advantages of low resistance, broad-spectrum action, safety and environmental protection of biological pesticides. The advantages are complementary and the control effect is doubled. The reasonable combination of biological pesticides and chemical pesticides is an important technical means to promote the reduction of pesticide use and increase efficiency, and to achieve green and sustainable development of agriculture. Detailed implementation manners

[0032] It should be noted that the following detailed description is exemplary and is intended to provide further illustration of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs. The embodiments described are only a part of the embodiments of the present invention, rather than all cases.

[0033] Indoor bactericidal activity test method:

[0034] 1. Test strains: Select appropriate test strains according to the test requirements.

[0035] 2. Test agents: Guanine glycoside was prepared in the laboratory, and 50% boscalid technical, 6% kasugamycin, and 10% ningnanmycin are all commercially available. After dissolving various chemical agents, they were diluted with 0.1% Tween-80 solution to prepare single-agent mother liquors. Multiple groups of ratios were set, and 5 mass concentration gradients were set for each single-agent mother liquor and the mixed agents of the ratios according to the equal ratio method.

[0036] 3. Test method

[0037] The mycelial growth rate method was adopted. The pre-melted culture medium was added to a sterile conical flask. 1 ml of the liquid medicine was quantitatively absorbed in turn from low concentration to high concentration and added to the above conical flask. After shaking well, it was poured into a petri dish with a diameter of 9 cm to make a drug-containing plate with the corresponding concentration. A treatment without the agent was set as a blank control, and 6 replicates were set for each treatment. A mycelial disc with a diameter of 5 mm was cut from the edge of the test strain colony and inoculated in the center of the drug-containing plate and the blank control plate, and then placed in a suitable incubator for cultivation. The diameter of the colony was measured by the cross method, and the inhibition rate of mycelial growth by different agent treatments was calculated.

[0038]

[0039] 4. Data analysis

[0040] SPSS software was used for data statistical analysis. Taking the logarithm value of the fungicide concentration as x and the probit value of the corresponding mycelial growth inhibition rate as y, a linear regression was performed to obtain the virulence regression equation, calculate the EC50 value of the virulence of the agent against the target pathogen, and calculate the co-toxicity coefficient (CTC) according to the Sun Yunpei method. The combined virulence effect of the two agents was judged by the CTC value. Less than 80 indicates antagonistic effect, 100 - 200 indicates additive effect between the agents, greater than 120 indicates synergistic effect, and 80 - 120 indicates additive effect.

[0041]

[0042] Theoretical toxicity index (TTI) = ∑(toxicity index of a certain agent × percentage of a certain agent in the mixture)

[0043]

[0044] Example 1: Indoor toxicity determination of different ratios of guanosine glycoside and boscalid against Botrytis cinerea of tomato

[0045] The Botrytis cinerea pathogen used in this experiment was preserved in the microbial library of Shandong Pengbo Biotechnology Co., Ltd. and activated with PDA medium for standby. Guanosine glycoside and boscalid were mixed in different ratios and tested according to the above indoor bactericidal activity test method. The results are as follows:

[0046] Table 1 Indoor biological activity determination of different ratios of guanosine glycoside and boscalid against Botrytis cinerea of tomato

[0047]

[0048] The results of indoor toxicity determination showed that when guanosine glycoside and boscalid were compounded at a mass ratio of 1:10 - 20:1, the co-toxicity coefficients for controlling Botrytis cinerea of tomato were all above 120, showing a synergistic effect. When the mass ratio of guanosine glycoside and boscalid was 1:5 - 10:1, the co-toxicity coefficient was greater than 140, with a significant synergistic effect, and the co-toxicity coefficient was the largest when the mass ratio of guanosine glycoside and boscalid was 1:2.

[0049] Example 2: Indoor toxicity determination of different ratios of guanosine glycoside and kasugamycin against bacterial leaf spot of tomato

[0050] The Pseudomonas syringae pv tomato (PstDC3000) used in this experiment was preserved in the microbial library of Shandong Pengbo Biotechnology Co., Ltd. and activated with KB medium containing rifampicin for standby. Guanosine glycoside and kasugamycin were mixed in different ratios and tested according to the above indoor bactericidal activity test method. The results are as follows:

[0051] Indoor bioactivity determination of different ratios of guanosine glycoside and kasugamycin against bacterial leaf spot of tomato (pstDC3000) in Table 2

[0052]

[0053] It can be seen from the indoor virulence determination results in Table 2 that after the compounding of guanosine glycoside and kasugamycin, the co-toxicity coefficient against bacterial leaf spot of tomato is greater than 120 when the mass ratio is 1:15 to 10:1, showing a synergistic effect. When the mass ratio is 5:1, the co-toxicity coefficient is 152.11, showing a significant synergistic effect.

[0054] Example 3: Indoor virulence determination of different ratios of guanosine glycoside and ningnanmycin against tobacco mosaic virus

[0055] 1. Test method

[0056] (1) Bacterial liquid activation: The TMV-infected diseased leaves used in this test were preserved by Shandong Pengbo Biotechnology Co., Ltd. Take out 0.1 g of the infected diseased leaves from the -80°C refrigerator, add 2 ml of PBS and grind them into a pulp to prepare the infected juice. Select 3 expanded tobacco leaves, use quartz powder for rubbing inoculation, observe the disease occurrence of the inoculated leaves under long-wave ultraviolet light, obtain fresh infected diseased leaves, and prepare fresh infected bacterial liquid.

[0057] (2) Virus inoculation: Compound guanosine glycoside and ningnanmycin in different ratios to make corresponding concentration medicament solutions, and uniformly spray them on the surface of tobacco leaves. 2 hours after the application of the medicament, select 3 expanded tobacco leaves, use quartz powder for rubbing inoculation (50 μL of virus juice is inoculated on each leaf), and 6 plants are inoculated for each treatment.

[0058] (3) Disease investigation and data statistics: Continue to culture for 5 days after inoculation, investigate the disease occurrence, calculate the relative control efficacy of the medicament based on the disease index, obtain the virulence regression equation, and calculate the co-toxicity coefficient of the mixed medicament.

[0059] The disease index is divided into 5 grades:

[0060] Grade 0: No mosaic;

[0061] Grade 1: Individual dark green spots appear on the leaves;

[0062] Grade 2: Individual leaves show slight mosaic;

[0063] Grade 3: 1 or 2 leaves show severe mosaic;

[0064] Grade 4: Most leaves show severe mosaic.

[0065]

[0066] 2. Test results

[0067] Table 3 Indoor Bioactivity Determination of Different Ratios of Guanine Glycoside and Ningnanmycin against Tobacco Mosaic Virus (TMV)

[0068]

[0069]

[0070] As can be seen from the results in Table 2, after the compounding of guanine glycoside and ningnanmycin, the co-toxicity coefficient against tomato bacterial leaf spot is greater than 120 when the mass ratio is 1:25 to 10:1, showing a synergistic effect. When the mass ratio is 1:25 to 1:5, the co-toxicity coefficient is greater than 140, showing a significant synergistic effect. Among them, the co-toxicity coefficient is the largest when the mass ratio of guanine glycoside and ningnanmycin is 1:5.

[0071] Example 4 Preparation of Different Pesticide Formulations of Guanine Glycoside Bactericidal Composition

[0072] Taking guanine glycoside and one of boscalid, kasugamycin, and ningnanmycin as active ingredients, a variety of pesticide formulations can be prepared, including: suspension concentrate, emulsifiable concentrate, water dispersible granules, microemulsion, wettable powder, etc., all of which are prepared by conventional methods. The following are some examples.

[0073] 1. 30% Guanine Glycoside · Boscalid Suspension Concentrate (1:2): Guanine Glycoside (active ingredient) 10%, Boscalid (active ingredient) 20%, Sodium Dodecyl Sulfate (wetting agent) 5.5%, Fatty Alcohol Polyoxyethylene Ether Phosphate (dispersant) 6%, Gum Arabic (thickener) 2.5%, Glycerol (antifreeze) 3%, Organosilicon Oil (defoamer) 0.3%, Deionized Water (solvent) to make up 100%. First, mix other auxiliaries, shear and mix them evenly in a shear machine, then add guanine glycoside and boscalid, and grind them in a ball mill for 2 hours to obtain 30% guanine glycoside · boscalid suspension concentrate.

[0074] 2. 30% Guanine Glycoside · Boscalid Water Dispersible Granules (1:2): Guanine Glycoside (active ingredient) 10%, Boscalid (active ingredient) 20%, Dispersant NNO 2%, Sodium Lignosulfonate 9%, Sodium Dodecyl Sulfate 4%, Bentonite to make up 100%. Mix all components evenly, grind them into a wettable powder in a conventional manner by air flow, and then add an appropriate amount of water to mix and extrude into granules. After drying and screening, 30% guanine glycoside · boscalid water dispersible granules are obtained.

[0075] 3.24% guanine glycoside·kasugamycin wettable powder (5:1): guanine glycoside (active ingredient) 20%, kasugamycin (active ingredient) 4%, sodium lauryl sulfate 2%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, kaolin to 100%. The ingredients are fully mixed and crushed in a superfine grinder in a conventional manner to obtain 24% guanine glycoside·kasugamycin wettable powder.

[0076] 4.24% guanine glycoside·kasugamycin water dispersible granules (5:1): guanine glycoside (active ingredient) 20%, boscalid (active ingredient) 4%, dispersant NNO 2%, sodium lignin sulfonate 9%, sodium lauryl sulfate 4%, bentonite to 100%. The ingredients are mixed evenly, air-blasted into wettable powder in a conventional manner, then an appropriate amount of water is added to mix, extruded and granulated, and water dispersible granules are obtained after drying and sieving.

[0077] 5.18% guanine glycoside·Ningnanmycin suspension (1:5): guanine glycoside (active ingredient) 3%, Ningnanmycin (active ingredient) 15%, calcium dodecylbenzenesulfonate (wetting agent) 3.5%, fatty alcohol polyoxyethylene ether phosphate (dispersant) 4.5%, xanthan gum (thickener) 2%, propylene glycol (antifreeze agent) 2%, silicone oil (defoaming agent) 0.3%, deionized water (solvent) is added to 100%; first mix other auxiliary agents, shear and mix evenly in a shearing machine, then add Ningnanmycin and fluopicolide, grind in a ball mill for 2 hours, and 18% guanine glycoside·Ningnanmycin suspension can be obtained.

[0078] 18% guanine glycoside·Ningnanmycin wettable powder (1:5): guanine glycoside (active ingredient) 3%, Ningnanmycin (active ingredient) 15%, sodium dodecyl sulfate 2%, sodium lignin sulfonate 5%, naphthalene sulfonate 3%, kaolin to 100%. The ingredients are fully mixed and crushed by a superfine grinder in a conventional manner to obtain 18% guanine glycoside·Ningnanmycin wettable powder.

[0079] Example 5 Verification of the Disease Control Effect of Guanine Glycoside Composition

[0080] 1. Test methods

[0081] The specific methods for selecting the above-mentioned agents for indoor control tests on tomato gray mold, Arabidopsis bacterial leaf spot, and tobacco mosaic virus diseases are as follows:

[0082] (1) Indoor control test of tomato gray mold: The gray mold pathogen strain is activated and set aside, and the prepared agent is evenly sprayed on the surface of tomato leaves. Collect the detached leaves 4 hours after application and inoculate with gray mold fungi. Select tomato seedlings with uniform growth. Take 3 leaves from each plant and place them on an inoculation tray, with 4 inoculation points on each leaf. Pierce a small hole in the midrib of the leaf, use a puncher with a diameter of about 5 mm to punch holes along the outer edge of the bacterial plate, use a needle to pick up the bacterial cake and place it on the wound on the leaf, and induce the disease in a culture room at 25℃ (16:8h) and 6000Lux. 5-7d after inoculation, observe the disease condition of the leaves, measure the area of ​​lesions, and calculate the efficacy of the agent. Gray mold disease grade grading standard: The severity of tomato gray mold disease is divided into 6 grades:

[0083] Level 0: healthy and asymptomatic;

[0084] Level 1: The lesion area accounts for 1%-25% of the total leaf area;

[0085] Level 2: The lesion area accounts for 25%-50% of the total leaf area;

[0086] Level 3: The lesion area accounts for 50%-75% of the total leaf area;

[0087] Level 4: The lesion area accounts for 75%-95% of the total leaf area;

[0088] Level 5: The diseased area accounts for more than 95% of the total leaf area.

[0089] (2) Indoor control test of bacterial leaf spot of Arabidopsis thaliana: Pseudomonas PstDC3000 strain was activated and treated with 10 mM MgCl 2 Resuspend and adjust the bacterial solution concentration to OD600 value of 0.0002 (approximately 2×10 5 cfu / ml). Select healthy and mature Arabidopsis leaves, mark them and inoculate them with a 1ml syringe without a needle until the bacterial solution covers the entire leaf. Inoculate 3-4 leaves per plant. Cut the leaves 4 days after inoculation, take 3-4 leaves from each plant and weigh them, put them into a 2ml centrifuge tube, and add 1ml 10mM MgCl 2 and small steel beads, and then broken on a tissue disruptor for 2 min. Use 10 mM MgCl 2 Dilute according to the concentration gradient, take 20μl of the bacterial solution of each concentration, drop it on the KB medium containing rifampicin, and culture it in a 28℃ incubator for 1-2 days until the colonies on the culture dish are clearly visible. Observe and record the colony count under an ordinary microscope, and calculate the unit bacterial content of the leaves and the protective effect of the agent.

[0090] (3) Indoor control test for tobacco virus diseases: Potato virus X (PVX) was provided by Shandong Pengbo Biotechnology Co., Ltd. Nicotiana benthamiana was selected as the test plant, and seedlings at the 4-8 leaf stage with the same size, growth vigor, and free of diseases and pests were selected for the test. The medicament should be evenly sprayed on both the front and back sides of the leaves, and continuous spraying should be carried out until the medicament is evenly sprayed on both the front and back sides of the leaves, so that the medicament reaches a saturated state on the leaf surface. A little quartz powder was evenly sprinkled on the front sides of the 3 expanded leaves under the incompletely expanded young leaves of the tobacco, and the virus was inoculated onto the 3 expanded leaves by friction inoculation (50 μL of PVX virus-containing juice was inoculated on each leaf, and the preparation ratio of the virus juice was (0.1 g of virus-infected leaves + 2 ml of PBS buffer) mixed and ground, and it could be prepared according to the actual required amount of virus solution). 6 plants were inoculated for each treatment, and each plant was used as 1 replicate. After inoculation, they were placed in an artificial climate chamber and continued to be cultured for 5 days. The disease incidence of the inoculated tobacco was observed under long-wave ultraviolet light and the results were photographed. Then, samples of the diseased young leaves of each plant were taken, and the virus expression level was detected by ELISA to calculate the virus inhibition rate.

[0091] Virus inhibition rate = [(control virus content - treated virus content) / control virus content] × 100%

[0092] 2. Test results

[0093] As can be seen from Table 4, the 6 compounded medicaments had significant synergistic effects compared with the single-agent controls. The control effects of the 30% guanylglycoside·boscalid suspension concentrate and the 30% guanylglycoside·boscalid water dispersible granule on tomato gray mold were increased by 62.83% and 52.89% respectively compared with 50% guanylglycoside, and increased by 45.65% and 36.76% respectively compared with 50% boscalid. The control effects of the 24% guanylglycoside·kasugamycin wettable powder and the 24% guanylglycoside·kasugamycin water dispersible granule on Arabidopsis thaliana bacterial leaf spot were increased by 89.48% and 102.69% respectively compared with 50% guanylglycoside, and increased by 30.31% and 39.40% respectively compared with 6% kasugamycin. The control effects of the 18% guanylglycoside·ningnanmycin suspension concentrate and the 18% guanylglycoside·ningnanmycin wettable powder on tobacco virus diseases were increased by 64.72% and 67.38% respectively compared with 50% guanylglycoside, and increased by 55.17% and 57.68% respectively compared with 10% ningnanmycin.

[0094] Table 4 Indoor control effects of guanylglycoside composition suspension concentrates on different diseases

[0095]

[0096] The test results show that the six formulated pesticides can effectively control fungal, bacterial, and viral diseases, with a significant synergistic effect. The control efficacy is significantly higher than that of single pesticides, thus reducing the amount of pesticides used, delaying the emergence of pesticide resistance, having a long residual period, being environmentally friendly, and meeting the safety requirements of compound pesticide formulations.

[0097] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various modifications and changes. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A fungicidal composition based on guanine glycoside, characterized in that The active ingredients include guanine glycoside and at least one of boscalid, kasugamycin and ningnanmycin.

2. The use of the guanine glycoside-based bactericidal composition according to claim 1, characterized in that: The application includes at least one of the following aspects: a) Prevent and control plant fungal diseases; b) Preventing and controlling bacterial diseases of plants; c) Prevent and control plant viral diseases.

3. A fungicidal composition for preventing and controlling plant fungal diseases, characterized in that: The active ingredients are guanine glycoside and boscalid, and the plant fungal disease is tomato gray mold.

4. The fungicidal composition for preventing and controlling plant fungal diseases according to claim 3, characterized in that: The mass ratio of guanine glycoside to boscalid is 1:10 to 20:

1.

5. The fungicidal composition for preventing and controlling plant fungal diseases according to claim 3, characterized in that: The mass ratio of guanine glycoside to boscalid is 1:5 to 10:

1.

6. A bactericidal composition for preventing and controlling plant bacterial diseases, characterized in that: The active ingredients are guanine glycoside and kasugamycin, and the plant bacterial disease is bacterial leaf spot.

7. The bactericidal composition for preventing and controlling plant bacterial diseases according to claim 6, characterized in that: The mass ratio of guanine glycoside to kasugamycin is 1:15 to 10:

1.

8. A composition for preventing and treating plant viral diseases, characterized in that: The active ingredients are guanine glycoside and ningnanmycin, and the plant virus disease is tobacco mosaic virus.

9. The composition for preventing and controlling plant viral diseases according to claim 8, characterized in that: The mass ratio of guanine glycoside to ningnanmycin is 1:25 to 10:

1.

10. The guanine glycoside-based bactericidal composition according to claim 1, characterized in that: The invention also includes an auxiliary agent, which includes at least one of a dispersant, an antifreeze agent, a thickener, a defoaming agent or a solvent.

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