Combined preparation containing intelligently assembled nicotinamide as well as preparation method and application of combined preparation

Abstract

The invention discloses a combined preparation containing intelligently assembled nicotinamide, belongs to the technical field of functional food, and solves the problem of unsatisfactory effect of whitening and anti-aging preparations in the prior art. The combined preparation containing the intelligently assembled nicotinamide is prepared from the following components in parts by mass: 5 to 12g of intelligently assembled nicotinamide GPC micro-capsule liquid, 2 to 3g of grape powder, 0.1 to 0.5 g of yeast extract, 0.03 to 0.05 g of vitamin C and 2 to 5g of red grape concentrated juice. The traditional action pathway is broken, the technical prejudice is overcome, and the anti-aging and whitening effects are realized from the mitochondrial cell layer.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and more particularly to a combined preparation containing intelligent assembly nicotinamide, a preparation method thereof and an application thereof. Background Art

[0002] In addition to being affected by internal factors such as age and genetics, skin aging is also affected by various external factors such as sunlight, environment, and health. The specific manifestation symptoms of skin aging include thinning of the skin, abnormal stratum corneum, dryness and water shortage, increased wrinkles, increased spots, dull skin color, lack of luster, etc.; among these manifestations, pigmentation and dull skin color are particularly prominent. The degree of skin dullness is closely related to the formation of melanin. Melanin is an important biological pigment that protects the human skin from ultraviolet radiation. It is affected by many factors such as genetics, endocrine, and sunlight exposure. Excessive production of melanin leads to skin pigmentation, which can cause problems such as skin dullness, darkening, and melasma. Therefore, a method of improving skin dullness by the combined action of anti-aging and reducing melanin accumulation is more scientific and effective.

[0003] The whitening mechanism of traditional edible preparations is mainly to inhibit melanin by hindering the activity of tyrosinase, and secondly to act on the biological transfer and metabolism of melanin; the action mechanisms of anti-aging drinking preparations are mostly to scavenge free radicals, resist glycation, anti-inflammatory, enhance immunity, and repair the skin barrier, etc. Their anti-aging and whitening effects only stay on the basic effects of the raw materials. Therefore, a product that can avoid production loss, has high bioavailability, has high anti-aging and whitening effects, and will not cause metabolic burden after long-term consumption is a very important and expected research direction. Summary of the Invention

[0004] The present invention provides a combined preparation containing intelligent assembly nicotinamide, a preparation method thereof and an application thereof, so as to solve the problem that the effect of existing whitening and anti-aging preparations is not ideal.

[0005] In a first aspect, the present invention provides a combined preparation containing intelligent assembly nicotinamide, which, in parts by mass, includes: 5-12 g of intelligent assembly nicotinamide GPC microcapsule liquid, 2-3 g of grape powder, 0.1-0.5 g of yeast extract, 0.03-0.05 g of vitamin C, and 2-5 g of red grape concentrate.

[0006] As a possible implementation, in parts by mass, it includes: 8 g of intelligent assembly nicotinamide GPC microcapsule liquid, 2.5 g of grape powder, 0.3 g of yeast extract, 0.04 g of vitamin C, and 3 g of red grape concentrate.

[0007] Second aspect, the present invention provides a preparation method of the combined preparation containing intelligent assembled niacinamide according to any possible implementation manner of the first aspect, comprising the following steps: dissolving the grape powder, the yeast extract, and the vitamin C in pure water, and stirring evenly; adding the red grape concentrate and the intelligent assembled niacinamide GPC microcapsule liquid, and performing high-speed shearing and stirring, then adding pure water until the soluble solid content is 10.6 - 11.0 and the pH is 2.88 - 3.28, and performing encapsulation and sterilization to obtain the combined preparation containing intelligent assembled niacinamide.

[0008] As a possible implementation manner, the preparation method of the intelligent assembled niacinamide GPC microcapsule liquid comprises the following steps: adding isomaltooligosaccharide and L-α-glycerylphosphorylcholine (GPC) to a gelatin solution, completely dissolving and mixing evenly under the condition of constant temperature at 40°C, adding niacinamide to the reaction system under stirring conditions, and continuing stirring to obtain a precursor of the intelligent assembled niacinamide GPC microcapsule liquid; detecting, volumetrically fixing, filling, and sealing to obtain the intelligent assembled niacinamide GPC microcapsule liquid.

[0009] As a possible implementation manner, in terms of mass ratio, the addition ratio of the isomaltooligosaccharide and the L-α-glycerylphosphorylcholine is (0.2 - 0.5):(0.03 - 0.05); the final content of niacinamide in the intelligent assembled niacinamide GPC microcapsule liquid is 0.16 - 0.20 mg / kg; the indexes and standards for the detection are: the soluble solid content is 8.5 ± 0.2, and the PH is 6.7 ± 0.2.

[0010] Third aspect, the present invention provides an application of the combined preparation containing intelligent assembled niacinamide according to any possible implementation manner of the first aspect or the combined preparation containing intelligent assembled niacinamide prepared by the preparation method according to any possible implementation manner of the second aspect in the preparation of a whitening and anti-aging drinking preparation.

[0011] As a possible implementation manner, the combined preparation containing intelligent assembled niacinamide is used for scavenging free radicals and / or for inhibiting the activity of tyrosinase.

[0012] Third aspect, the present invention provides a whitening and anti-aging drinking preparation, comprising the combined preparation containing intelligent assembled niacinamide according to any possible implementation manner of the first aspect or the combined preparation containing intelligent assembled niacinamide prepared by the preparation method according to any possible implementation manner of the second aspect, and excipients acceptable in drinking products.

[0013] As a possible implementation manner, the excipients acceptable in drinking products include but are not limited to: sweeteners, acidulants, flavors / spices, pigments, preservatives, antioxidants, emulsifiers, thickeners, enzyme preparations, carbon dioxide.

[0014] As a possible implementation, it is prepared as powder, granule, tablet, solution, suspension, colloid, paste.

[0015] The combination preparation provided by the present invention overcomes technical prejudice, breaks the traditional action pathway, and exerts the effects of anti-aging and whitening from the mitochondrial cell level: Nicotinamide (NAM), as a precursor of NAD + , enters the body to promote the generation of NAD + ; NAD + can eliminate superoxide anion radicals, inhibit protease-activated receptor 2 (PAR-2), prevent the transmission of melanin, accelerate the metabolism of melanin, promote collagen synthesis, supplement NAD + in the body, promote mitochondrial repair, delay cell aging, improve skin condition; act from the cell level, delay aging from the inside out, and last without rebound.

[0016] The nicotinamide in the combination preparation provided by the present invention is different from the traditional nicotinamide source. The preparation method of the present invention is simple in operation, short in time-consuming, free of organic reagents, and free of high-temperature treatment, effectively protecting the activity of nicotinamide. The core raw material of the combination preparation provided by the present invention is the intelligent assembly nicotinamide GPC microcapsule liquid, which can better exert the effects of whitening and anti-aging, and has the characteristics of stronger efficacy, lower cost, and higher safety.

[0017] In the intelligent assembly nicotinamide GPC microcapsule liquid, which is the raw material of the combination preparation provided by the present invention, the nicotinamide can withstand high-temperature treatment during production and the action of gastrointestinal enzymes, and the loss of active ingredients is significantly reduced.

[0018] The combination preparation containing intelligent assembly nicotinamide provided by the present invention has the effects of delaying cell aging, scavenging free radicals, inhibiting melanin synthesis, and accelerating melanin metabolism. The dosage is low, there is no metabolic burden, diseases caused by long-term use are effectively avoided, it is beneficial to long-term whitening and anti-aging, and it is suitable for the general population to take. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0020] Figure 1 It is the test result of free radical scavenging performance provided by the embodiment of the present invention.

[0021] Figure 2 It is the test result of the ability to inhibit tyrosinase provided by the embodiment of the present invention. Detailed implementation manners

[0022] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0023] To solve the problem that the anti-aging preparations for Sino-US white have unsatisfactory effects in the prior art, the present invention provides a combined preparation containing intelligent assembled nicotinamide, its preparation method and application.

[0024] The raw materials or chemical reagents used in the present invention, unless otherwise specified, are all obtained through conventional commercial channels. The sources of some raw materials are as follows: nicotinamide: Shandong Jinchengda Biotechnology Co., Ltd.; L-α-glycerylphosphorylcholine: Shandong Aobo Biotechnology Co., Ltd.; isomaltooligosaccharide: Shandong Bailong Chuangyuan Biotechnology Co., Ltd.; grape powder (5% resveratrol): Shaanxi Angxu Biotechnology Co., Ltd.; yeast extract (glutathione); Angel Yeast Co., Ltd.; vitamin C: Henan Bloomage Biotechnology Co., Ltd.; red grape concentrate: Tianjin Yuepengtai Food Co., Ltd.; gelatin: Rosette.

[0025] Next, the technical solutions of the present invention will be further elaborated in conjunction with specific embodiments.

[0026] Example 1

[0027] This example provides the preparation of a combined preparation containing intelligent assembled nicotinamide.

[0028] Add 0.2 - 0.5 kg of isomaltooligosaccharide and 0.03 - 0.05 kg of L-α-glycerylphosphorylcholine (GPC) to a gelatin solution (obtained by dissolving 0.05 kg of gelatin powder in 4.2 kg of water), completely dissolve and mix at a constant temperature of 40°C. Under stirring conditions, add NAM to the reaction system to make its final concentration 0.16 - 0.20 mg / kg, and continue stirring for 1 h to obtain an intelligent assembled microexplosion bead mixture; detect (detection index: soluble solids content is 8.5 ± 0.2, PH is 6.7 ± 0.2), make up the volume to 10 kg, and obtain an intelligent assembled nicotinamide GPC microcapsule liquid (solution embedding nicotinamide) after filling and sealing.

[0029] Dissolve 2 - 3 g of grape powder (5% resveratrol), 0.1 - 0.5 g of yeast extract (glutathione), and 0.03 - 0.05 g of vitamin C in 10 g of pure water, and stir for 5 min; sequentially add 2 - 5 g of red grape concentrate and 5 - 6 g of the prepared intelligent assembled nicotinamide GPC microcapsule liquid into the reaction system, perform high-speed shear stirring for 10 min, and make up the volume to 100 g with pure water. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2; perform 80-mesh filtration, homogenization, and ultra-high temperature instantaneous sterilization in sequence and then conduct detection. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2. If it meets the standard, then fill and seal, and sterilize to obtain a combined preparation containing intelligent assembled nicotinamide.

[0030] Example 2

[0031] This example provides the preparation of a combined preparation containing intelligent assembled nicotinamide.

[0032] Add 0.2 - 0.5 kg of isomaltooligosaccharide and 0.03 - 0.05 kg of L-α-glycerylphosphorylcholine (GPC) to a gelatin solution (obtained by dissolving 0.05 kg of gelatin powder in 4.2 kg of water), completely dissolve and mix evenly under the condition of constant temperature at 40 °C. Add NAM to the reaction system under stirring conditions to make its final concentration 0.16 - 0.20 mg / kg, and continue stirring for 1 h to obtain an intelligent assembled microcapsule bead mixture; conduct detection (detection indicators: soluble solids content is 8.5 ± 0.2, PH is 6.7 ± 0.2), make up the volume to 10 kg, and after filling and sealing, obtain intelligent assembled nicotinamide GPC microcapsule liquid.

[0033] Dissolve 2 - 3 g of grape powder (5% resveratrol), 0.1 - 0.5 g of yeast extract (glutathione), and 0.03 - 0.05 g of vitamin C in 10 g of pure water, and stir for 5 min; sequentially add 2 - 5 g of red grape concentrate and 10 - 12 g of the prepared intelligent assembled nicotinamide GPC microcapsule liquid into the reaction system, perform high-speed shear stirring for 10 min, and make up the volume to 100 g with pure water. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2; perform 80-mesh filtration, homogenization, and ultra-high temperature instantaneous sterilization in sequence and then conduct detection. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2. If it meets the standard, then fill and seal, and sterilize to obtain a combined preparation containing intelligent assembled nicotinamide.

[0034] Example 3

[0035] This example provides the preparation of a combined preparation containing intelligent assembled nicotinamide.

[0036] Add 0.3 kg of isomaltooligosaccharide and 0.04 kg of L-α-glycerylphosphorylcholine to a gelatin solution (obtained by dissolving 0.05 kg of gelatin powder in 4.2 kg of water), completely dissolve and mix them under the condition of constant temperature at 40°C. Add NAM to the reaction system under stirring conditions to make its final concentration 0.16 - 0.20 mg / kg, and continue stirring for 1 h to obtain a smart-assembled micro-explosion bead mixture; detect (detection index: soluble solid content is 8.5, PH is 6.7), make up the volume to 10 kg, and obtain a smart-assembled nicotinamide GPC microcapsule solution after filling and sealing.

[0037] Dissolve 2.5 g of grape powder (5% resveratrol), 0.3 g of yeast extract (glutathione), and 0.04 g of vitamin C in 10 g of pure water, and stir for 5 min; sequentially add 3 g of red grape concentrate and 8 g of the prepared smart-assembled nicotinamide GPC microcapsule solution to the reaction system, and perform high-speed shearing and stirring for 10 min. Make up the volume to 100 g with pure water. It is required that the soluble solid content of the product is 10.8 and the pH is 3.08; perform 80-mesh filtration, homogenization, and ultra-high temperature instantaneous sterilization in sequence and then detect. It is required that the soluble solid content of the product is 10.8 and the pH is 3.08. If it meets the standard, fill and seal, and sterilize to obtain a combined preparation containing smart-assembled nicotinamide.

[0038] Comparative Example 1

[0039] This example provides the preparation of a combined preparation.

[0040] Dissolve 2 - 3 g of grape powder (5% resveratrol), 0.1 - 0.5 g of yeast extract (glutathione), and 0.03 - 0.05 g of vitamin C in 10 g of pure water, and stir for 5 min; add 2 - 5 g of red grape concentrate to the reaction system, and perform high-speed shearing and stirring for 10 min. Make up the volume to 100 g with pure water. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2; perform 80-mesh filtration, homogenization, and ultra-high temperature instantaneous sterilization in sequence and then detect. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2. If it meets the standard, fill and seal, and sterilize to obtain a combined preparation without smart-assembled nicotinamide.

[0041] Comparative Example 2

[0042] This example provides the preparation of a combined preparation.

[0043] Dissolve 2 - 3 g of grape powder (5% resveratrol), 0.1 - 0.5 g of yeast extract (glutathione), and 0.03 - 0.05 g of vitamin C in 10 g of pure water, and stir for 5 min; sequentially add 2 - 5 g of red grape concentrate and 0.003 g of niacinamide into the reaction system, perform high-speed shearing and stirring for 10 min, and make up the volume to 100 g with pure water. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2; perform 80-mesh filtration, homogenization, and ultra-high temperature instantaneous sterilization in sequence and then conduct detection. It is required that the soluble solid content of the product is 10.8 ± 0.2 and the pH is 3.08 ± 0.2. If it meets the standard, fill and seal, and sterilize to obtain a combined preparation containing niacinamide.

[0044] Example 4

[0045] This example provides a test on the free radical scavenging performance of a combined preparation.

[0046] Perform free radical scavenging tests on the combined preparations containing intelligent assembled niacinamide prepared in Examples 1 - 4 respectively. The experiments are as follows:

[0047] DPPH solution (the solvent is absolute ethanol, and the concentration is 0.05 g / L), VC solution (the solvent is physiological saline, and the concentration is 1 g / L). Use the VC solution as the positive control, and add reagents into the 96-well plate according to Table 1.

[0048] Table 1 Types of reagents added to the 96-well plate

[0049]

[0050] Vortex and mix each test tube, let it stand in the dark at room temperature for 30 min, and measure its absorbance at 517 nm. One control tube needs to be set for each test tube. The blank tube is measured 1 - 2 times.

[0051] Calculate the DPPH free radical scavenging rate according to the following formula: P% = [1 - (As - Ac) / (Ab - A blank)] × 100%; obtain the results shown in Table 2 and Figure 1 In Table 2, the unit of the concentration in the first column is μg / mL.

[0052] Among them, P% represents the DPPH free radical scavenging rate, As represents the absorbance of the mixture of the test solution and the DPPH solution; Ac represents the absorbance of the mixture of the test solution and the absolute ethanol solution; Ab represents the absorbance of the mixture of the DPPH solution and the sample solvent solution; A blank represents the absorbance of the blank tube.

[0053] Table 2 Results of DPPH free radical scavenging rate (%)

[0054] Concentration Positive control VC Example 1 Example 2 Example 3 Comparative example 1 Comparative example 2 500 59.20±0.15 67.17±0.43 77.74±0.23 80.45±0.17 60.53±0.34 64.23±0.52 250 54.80±0.23 66.23±0.46 75.34±0.43 78.29±0.21 57.99±0.32 62.13±0.43 125 50.20±0.33 63.87±0.51 74.25±0.53 75.37±0.34 55.30±0.13 60.02±0.34

[0055] As shown in Table 2 and Figure 1 it can be seen that the composition preparations with different components and additives at different concentrations all have the ability to scavenge free radicals. Among them, compared with the positive control group, the ability of Examples 1, 2, and 3 to scavenge free radicals is significantly enhanced, and the scavenging ability of Example 3 is the strongest. Compared with the positive group, for Comparative Example 1 and Comparative Example 2, the effect of scavenging free radicals is significantly better than that of the positive control. Compared with Examples 1 to 3, the ability of Examples 1 to 3 to scavenge free radicals is stronger; the results of the free radical scavenging ability test show that the combined preparation containing intelligent assembled nicotinamide prepared by the present invention can promote the enhancement of the free radical scavenging ability under certain concentration conditions.

[0056] Example 5

[0057] This example provides a test for the ability of a combined preparation to inhibit tyrosinase.

[0058] Tyrosinase, also known as polyphenol oxidase, is a copper-containing redox enzyme of about 75 ku, which is widely present in animals, plants, microorganisms and the human body. It is the rate-limiting enzyme for melanin synthesis and directly affects the synthesis of melanin. The expression and activity of tyrosinase determine the rate and yield of melanin production. The higher the activity of tyrosinase, the more melanin is formed in the skin. The experiment of detecting tyrosinase activity in vitro can detect the effect on skin whitening.

[0059] The combined preparations containing intelligent assembled nicotinamide prepared in Examples 1 to 4 were respectively tested for free radical scavenging. The experiment is as follows:

[0060] Phosphate buffer (NaH 2 PO 4 ·2H 2 O and Na 2 HPO 4 ·12H 2 O with a molar ratio of 1:1, pH of 6.8, and a molar concentration of 1 / 15 mmol·L -1 ), mushroom tyrosinase solution (the solvent is phosphate buffer solution, with a molar concentration of 0.2 mg·mL -1 ), L-DOPA solution (the solvent is phosphate buffer solution, with a molar concentration of 2.0 mmol·L -1 ), and VC solution (concentration of 0.5 mg·mL -1 ) were used as the positive control group. The test sample solutions of Examples 1 to 4 were diluted with phosphate buffer to a concentration of 0.5 mg·mL -1 .

[0061] The reaction was carried out in a 96-well plate. 50 μL of mushroom tyrosinase solution, the test sample solutions of Examples 1 to 4, phosphate buffer solution, and L-DOPA solution were added in sequence, and the total volume of the reaction system was 200 μL. At the same time, a control group was set up, and 50 μL of mushroom tyrosinase solution, 100 μL of phosphate buffer solution, and 50 μL of L-DOPA solution were added in sequence. After the sample addition was completed, the mixture was immediately shaken and mixed evenly for 5 s using a multi-functional microplate reader, and the OD value of the reaction system at a wavelength of 475 nm was recorded under the constant temperature condition of 37°C. When the enzymatic reaction showed first-order kinetics, the slope of the straight line was the enzyme activity. Each group was parallelized for 3 experiments, and the average value was taken. The relative enzyme activity was calculated according to the following formula: relative enzyme activity (%) = Vs / Vc × 100, and the results shown in Table 3 and Figure 2 are as follows.

[0062] Among them, Vc represents the enzyme activity of the control group (OD 475 ·min -1 ), and Vs represents the enzyme activity of the sample group (OD 475 ·min -1 ).

[0063] Table 3 Relative enzyme activity test results (%)

[0064]

[0065] As can be seen from Table 3 and Figure 2 , the compositions and formulations with different ratios and different concentrations all have the ability to inhibit tyrosinase activity. Among them, compared with the blank group, the ability of Examples 1, 2, and 3 to inhibit tyrosinase activity exceeded 50%. Compared with the positive control group, the ability of Examples 1, 2, and 3 to inhibit tyrosinase activity was significantly enhanced, and compared with Comparative Example 1 and Comparative Example 2, the ability to inhibit tyrosinase activity was stronger. Compared with the positive control group, the ability of Comparative Example 1 and Comparative Example 2 to inhibit tyrosinase activity was somewhat enhanced. The results of the tyrosinase activity inhibition test show that the combination preparation containing intelligent-assembled nicotinamide prepared by the present invention can promote the enhancement of tyrosinase activity inhibition.

[0066] Although the preferred embodiments of the present invention have been described, those skilled in the art can make additional changes and modifications to these embodiments once they know the basic creative concepts. Therefore, the appended claims are intended to be construed to include the preferred embodiments as well as all changes and modifications that fall within the scope of the present invention.

[0067] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalent technologies, the present invention is also intended to include these modifications and variations.

Claims

1. A combination preparation containing intelligent assembly nicotinamide, characterized in that: Calculated by mass, it includes: 5-12g of smart assembly nicotinamide GPC microcapsule liquid, 2-3g of grape powder, 0.1-0.5g of yeast extract, 0.03-0.05g of vitamin C, and 2-5g of red grape concentrated juice.

2. The combined preparation containing intelligent assembly nicotinamide according to claim 1, characterized in that: Calculated by mass, it includes: 8g of smart assembly niacinamide GPC microcapsule liquid, 2.5g of grape powder, 0.3g of yeast extract, 0.04g of vitamin C, and 3g of red grape concentrated juice.

3. The method for preparing the combined preparation containing nicotinamide according to any one of claims 1 to 2, characterized in that: The following steps are involved: Dissolve the grape powder, the yeast extract and the vitamin C in pure water and stir evenly; The red grape concentrated juice and the smart-assembled nicotinamide GPC microcapsule liquid are added, high-speed shear stirring is performed, pure water is added until the soluble solid content is 10.6-11.0 and the pH is 2.88-3.28, and the combined preparation containing the smart-assembled nicotinamide is obtained after packaging and sterilization.

4. The preparation method according to claim 3, characterized in that: The preparation method of the intelligent assembly nicotinamide GPC microcapsule liquid comprises the following steps: Add isomaltooligosaccharide and L-α-phosphoglycerate choline to the gelatin solution, completely dissolve and mix at a constant temperature of 40°C, add nicotinamide to the reaction system under stirring conditions, continue stirring, and obtain a smart assembly nicotinamide GPC microcapsule liquid precursor; After testing, volume determination, filling and sealing, the intelligently assembled nicotinamide GPC microcapsule liquid is obtained.

5. The preparation method according to claim 4, characterized in that: In terms of mass ratio, the ratio of the added amount of the isomaltooligosaccharide to the added amount of the L-α-glycerophosphorylcholine is (0.2-0.5):(0.03-0.05); The final content of nicotinamide in the smart assembly nicotinamide GPC microcapsule solution is 0.16-0.20 mg / kg; The detection indexes and standards are: soluble solid content is 8.5±0.2, and pH is 6.7±0.

2.

6. Use of the combined preparation containing intelligently assembled nicotinamide according to any one of claims 1 to 2 or the combined preparation containing intelligently assembled nicotinamide prepared by the preparation method according to any one of claims 3 to 5 in the preparation of whitening and anti-aging drinking preparations.

7. The use according to claim 6, characterized in that: The combined preparation containing intelligent assembly nicotinamide is used for scavenging free radicals and / or for inhibiting the activity of tyrosinase.

8. A whitening and anti-aging drinking preparation, characterized in that: The invention comprises the combination preparation containing intelligently assembled nicotinamide according to any one of claims 1 to 2 or the combination preparation containing intelligently assembled nicotinamide prepared by the preparation method according to any one of claims 3 to 5, and excipients acceptable in drinking products.

9. The whitening and anti-aging drinkable preparation according to claim 8, characterized in that: Acceptable auxiliary materials in the drinking product include, but are not limited to, sweeteners, acidulants, flavors / spices, pigments, preservatives, antioxidants, emulsifiers, thickeners, enzyme preparations, and carbon dioxide.

10. The whitening and anti-aging drinking preparation according to claim 8, characterized in that: It is prepared into powder, granules, tablets, solutions, suspensions, colloids and pastes.

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