Sample collection device and sample detection method

Abstract

The invention provides a sample collection device and a detection method.The sample collection device comprises a collection bottle, a sampler and a separation part, a sample inlet and a sample outlet are formed in the two opposite ends of the sample collection device respectively, a through hole of the separation part is located in the lower portion of the sample inlet, and a sampling rod is inserted into the collection bottle from the sample inlet through the through hole of the separation part; and the liquid suction head is a quantitative capillary tube. The sample collection device is adopted in the detection process, so that the sample collection and storage are convenient, and the test sample can be quantitatively sucked.

Description

Technical Field

[0001] The present invention relates to the technical field of medical detection instruments, and particularly to a device for sample collection and quantitative sampling, as well as a detection method using the sample collection device. Background Art

[0002] Feces, as human waste, becomes a detection sample for indicators related to intestinal diseases, with the advantages of less damage to the human body and convenient sampling. For example, for the detection of intestinal pathogens such as intestinal adenopathy, rotavirus, and Clostridium difficile, and the detection of intestinal physicochemical functions such as fecal occult blood and calprotectin, feces are preferably used as the detection sample. Due to the complex composition and large individual differences of fecal samples, and the low content of substances to be detected, generally, a small amount of sample needs to be collected from the original feces with a sampling rod before testing, then the sample is diluted and mixed, and finally a certain amount of the mixed solution is taken for the detection of physiological indicators such as calprotectin.

[0003] For example, US Patent No. US6780160 is a sample collection patent for quantitatively collecting samples. A diaphragm is installed in a collection bottle filled with a buffer solution, and a through hole is provided in the middle of the diaphragm. The sampling part of the sampling rod is designed with a spiral groove. When the sampling rod is inserted into the collection bottle through the through hole, the diaphragm scrapes off the excess sample attached to the area outside the groove of the sampling rod, thus realizing the quantitative collection of fecal samples. The sample attached to the sampling rod and passing through the through hole is mixed with the buffer solution in the collection bottle to form a mixed solution. When using the mixed solution as a sample for detection, the tip of the closed dropper at the bottom of the collection bottle needs to be broken, and the mixed solution is extruded from the dropper. However, this process is likely to contaminate the operator, and the volume of the liquid droplets extruded from the dropper cannot be controlled, so quantitative dripping of the sample mixed solution cannot be achieved. Summary of the Invention

[0004] The present invention provides a new sample collection device, which can achieve quantitative sampling, quantitative dripping of the sample mixed solution, and avoid contaminating the operator during the operation.

[0005] Specifically, a sample collection device provided by the present invention includes a collection bottle, a sampler, and a separator. The opposite ends of the sample collection device are respectively provided with a sample inlet and a sample outlet end. The separator is connected to the sample inlet in the collection bottle, and the through hole of the separator is located below the sample inlet. The sampling rod of the sampler is inserted into the collection bottle through the through hole of the separator from the sample inlet. The pipettor includes a liquid suction head and a holding part. The liquid suction head is set as a quantitative capillary, and the liquid suction head is inserted into the collection bottle from the sample outlet end.

[0006] Further, the sampler includes a sampling rod, a sampling head located at the end of the sampling rod, and a holding part. The outer wall of the sampling head is provided with recesses at intervals. The sampler is connected to the collection bottle by inserting the sampling rod through the sample inlet and the through hole of the separator into the collection bottle.

[0007] Further, a sampler holder is provided at the sample outlet end of the sample collection device. The sampler holder further includes a sample outlet and a storage cavity. One end of the storage cavity is open, and the other end facing the inside of the collection bottle is closed.

[0008] Further, corresponding to the sampler holder, the sampler includes a liquid suction head, a gripping part, and a stopper. After the sampler is inserted into the sampler holder, the liquid suction head of the sampler is located in the storage cavity, and the stopper of the sampler is plugged into the sample outlet of the holder and seals the sample outlet liquid-tightly.

[0009] In this design, when assembling the sample collection device, to prevent the liquid suction head of the sampler from not being inserted into the sample outlet and the stopper of the sampler from not being inserted into the storage cavity as required, the opening sizes of the sample outlet of the sampler holder and the opening of the liquid suction head storage cavity can be made different.

[0010] Further, the stopper of the sampler and the sample outlet of the sampler holder are in interference fit to prevent liquid from flowing out of the sample outlet during the storage of the reagent or the temporary storage of the mixed sample.

[0011] Further, the sampler and the sampler holder can be connected by plugging and unplugging.

[0012] Further, when sucking the sample in the collection bottle with the liquid suction head of the sampler, the liquid suction head of the sampler is inserted from the sample outlet into the collection bottle.

[0013] Further, the opening size of the through hole of the separating member is substantially equivalent to or slightly smaller than the cross-sectional size of the sampling head.

[0014] Further, the cross-sectional size of the sampling head is slightly smaller than the cross-sectional size of the sampling rod.

[0015] Further, the sampling rod of the sampler and the through hole of the separating member are in interference fit.

[0016] Further, the sampler may further include a connecting arm, and the liquid suction head is connected to the gripping part through the connecting arm.

[0017] Further, the front end of the liquid suction head is provided with a liquid suction port, and the rear end is provided with a liquid outlet port, and there is a certain space above the liquid outlet port.

[0018] The present invention also provides a detection method, including the following steps:

[0019] (1) Take out the sample collection device. The sample collection device includes a collection bottle, a sampler, and a separating member. The opposite ends of the sample collection device are respectively provided with a sample inlet and a sample outlet end. The separating member is connected to the sample inlet in the collection bottle. The through hole of the separating member is located below the sample inlet. The sampler includes a liquid suction head and a gripping part. The liquid suction head is set as a quantitative capillary; the sampler is installed on the sample inlet, and the sampler is installed at the sample outlet end;

[0020] (2) Pull out the sampler from the sampling port and insert the sampling head of the sampler into the sample to take a sample;

[0021] (3) inserting the sampler containing the sample back into the collection bottle through the through hole of the separation piece, so that the sample on the sampling head and the reagent in the bottle are mixed to form a mixed sample;

[0022] (4) Turn over the sample collection device with the sample outlet facing upward, and remove the sample injector containing the mixed sample;

[0023] (5) The sample adder adds the mixed sample to the sample adding area of ​​the test card;

[0024] (6) The test card completes the test of the sample.

[0025] Furthermore, a sample adder bracket is provided at the sample outlet end of the sample collection device, and the sample adder bracket includes a sample outlet and a storage cavity, one end of the storage cavity is open, and the other end of the storage cavity facing the inside of the collection bottle is closed.

[0026] Furthermore, the matching sample injector comprises a liquid pipetting head, a gripping part and a plug. The liquid pipetting head of the sample injector is stored in the storage cavity, and the plug of the sample injector is plugged in the sample outlet of the bracket.

[0027] Furthermore, before forming the mixed sample, the pipette head of the pipette is stored in the storage chamber, and the stopper of the pipette is sealed in the sample outlet of the pipette bracket; after forming the mixed sample, the pipette is separated from the pipette bracket, and the pipette head is inserted into the collection bottle through the sample outlet of the pipette bracket; the capillary of the pipette head absorbs a quantitative mixed sample.

[0028] The present invention also provides a detection kit, which comprises a detection card and the sample collection device of the present invention.

[0029] Furthermore, the test includes a sample addition hole and an observation window, and a test paper is installed in the test card.

[0030] Furthermore, a diluent hole is provided upstream of the sample addition hole of the detection card, and an observation window is provided downstream of the sample addition hole.

[0031] Furthermore, the test strip is a lateral flow test strip.

[0032] By using the cooperation between the sampling head of the sample collection device of the present invention and the through hole of the separation member, quantitative collection of the sample is achieved, so that the collected sample is mixed with the reagent in the collection bottle according to a predetermined ratio; the capillary liquid suction head of the pipettor can accurately suck the mixed sample, and the mixed sample will not dirty the operator's hands; the interference fit between the sampling rod of the sampler and the through hole of the separation member, and the interference fit between the stopper of the pipettor and the sample outlet of the pipettor bracket can prevent the liquid in the collection bottle from leaking out. Before taking the mixed sample, the liquid suction head of the pipettor is temporarily stored in the liquid suction head storage cavity, so that the liquid suction head of the pipettor does not come into contact with the liquid in advance, which can avoid the situation that it is not easy for the mixed sample to enter the liquid suction head because the liquid suction head has sucked the solution in advance. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 FIG. is a schematic diagram of a sample collection device of the present invention, and the body of the collection bottle is made of a transparent material.

[0034] Figure 2 is Figure 1 exploded view of the sample collection device shown.

[0035] Figure 3 FIG. is a schematic diagram of the collection bottle of another sample collection device of the present invention.

[0036] Figure 4 is Figure 3 A-A sectional view in the direction of.

[0037] Figure 5 FIG. is a sectional view of the sampler and the pipettor inserted into the collection bottle and filled with reagent.

[0038] Figure 6 is Figure 5 exploded view of the first angle of the sample collection device shown.

[0039] Figure 7 is Figure 5 exploded view of another angle of the sample collection device shown.

[0040] Figure 8 FIG. is a schematic diagram of the pipettor separated from the collection bottle.

[0041] Figure 9 FIG. is a schematic diagram of the sampler taken out of the collection bottle.

[0042] Figure 10 FIG. is a schematic diagram of the sampler inserted into the sample.

[0043] Figure 11 FIG. is a schematic diagram of the sampler reinserted into the collection bottle.

[0044] Figure 12 FIG. is a schematic diagram of the sample collection bottle being flipped after the sample is collected.

[0045] Figure 13 It is a schematic diagram of the sampler taking out from the collection bottle.

[0046] Figure 14 It is a schematic diagram of the liquid suction head of the sampler inserting into the collection bottle to aspirate the solution.

[0047] Figure 15 It is a schematic diagram of the liquid suction head containing the sample solution adding sample to the test card.

[0048] Figure 16 It is a schematic diagram of the liquid suction head containing the sample solution inserting into the sample adding hole of the test card. Specific implementation mode

[0049] Such as Figure 1 And Figure 2 The sample collection device 1 shown in the figure includes a collection bottle 10, a sampler 20 and a sampler 30. The collection bottle 10 is provided with a sample inlet 11 and a sample outlet end. The sample outlet end has a sample outlet 12 and a separating member 13. The separating member is located inside the collection bottle and is connected to the sample inlet. The sampler 20 is inserted into the bottle through the sample inlet 11 via the separating member 13, and the liquid suction head 31 of the sampler 30 is inserted into the bottle through the sample outlet 12.

[0050] The sampler 20 is inserted into the collection bottle 10 through the sample inlet 11. The sample is transferred into the collection bottle 10 through the sampler 30. The sampler 30 is inserted into the collection bottle through the sample outlet 12 to obtain the liquid sample in the collection bottle.

[0051] The sampler 20 includes a sampling head 21, a sampling rod 22 and a holding part 23. The sampling head 21 is the lower part of the sampling rod 22. The sampling head 21 includes recessed parts 211 arranged at intervals on the outer wall. Of course, the shape of the sampling head 21 can also be other shapes, such as formed by a spiral groove or a long strip groove provided on the outer wall of the end of the sampling rod 22. During sampling, the sampling head 21 is inserted into samples such as feces, so that the sample is stored in the concave surface or groove and other recessed parts 211 of the sampling head.

[0052] In an embodiment where the inlet and the separation member are integrally formed, the inlet 11 is located above the separation member 13. The separation member 13 extends in the direction of the collection bottle to form a funnel-shaped structure. The opening size of the lower through-hole 131 of the funnel shape is approximately equivalent to or slightly smaller than the cross-sectional size of the sampling head 21. When the sampler with the fecal sample attached is inserted into the collection bottle 10 through the through-hole 131 of the separation member 13 via the inlet 11, the inner edge of the lower through-hole 131 of the separation member contacts the sample on the sampling head 11, thereby scraping off the excess sample outside the recessed portion 211 of the sampling head, ensuring that a fixed amount of the sample to be detected can be collected. The fixed amount of the sample is the sample stored in the recessed portion 211 of the sampling head. The excess sample scraped off from the sampling head can accumulate in the upper region 132 of the funnel. The combined member of the integrally formed inlet 11 and the separation member 13 can be assembled to one end of the collection bottle body by ultrasonic welding. The separation member can also be integrally formed with the collection bottle body with an inlet.

[0053] In an embodiment where the separation member 13 is an independent component, the collection bottle 10 includes an inlet 11. The separation member 13 is a funnel-shaped structure. The separation member is connected to the inlet 11. Specifically, the upper part 132 of the funnel is in close fit with the inner wall of the inlet 11. For example, an O-ring is installed on the outer wall of the separation member. In an embodiment where the separation member 13 is a T-shaped structure, the through-hole 131 of the separation member is provided at the "│" of the T-shape, and the "-" of the separation member is clamped to the upper end of the bottle body. A certain space can also be left between the "-" of the separation member and the upper end wall of the bottle body for collecting the scraped-off excess sample.

[0054] In an embodiment where the cross-sectional size of the sampling head 21 of the sampler is slightly smaller than the cross-sectional size of the sampling rod 22, there is an interference fit between the sampling rod 22 and the through-hole 131 of the separation member. When the sampling head is completely inserted into the collection bottle and contacts the reagent, since the outer wall of the sampling rod is in close contact with the inner wall of the through-hole of the separation member, it ensures that the reagent liquid in the collection bottle is in a reliable sealed state and will not flow out from the through-hole of the separation member.

[0055] A lid can be separately configured for the inlet 11, or the holding part 23 of the sampler can be designed as the lid of the inlet. After the sampler is inserted into the bottle body, the holding part 23 with the lid function is used to cover the inlet 11 to prevent the liquid in the collection bottle from flowing out through the inlet.

[0056] The pipette 30 includes a liquid suction head 31 and a gripping part 33. The pipette may also include a connecting arm 32, and the liquid suction head 31 is connected to the gripping part 33 through the connecting arm 32. In the embodiment where the liquid suction head 31 is of a capillary structure, a liquid suction port 311 is provided at the front end of the capillary structure, and a liquid outlet 312 is provided at the rear end. A certain space is left above the liquid outlet so that the gas in the liquid suction head can be discharged from the liquid outlet. When the sampling head with the sample is completely inserted into the collection bottle and contacts the reagent in the collection bottle to form a mixed solution, the sample collection device is flipped, and the liquid suction port of the pipette liquid suction head contacts the mixed solution, and then the sample in the liquid suction head can be transferred to a detection device (such as a test card).

[0057] A lid can be separately configured for the sample outlet 12, or the gripping part 33 of the pipette can be designed as the lid of the sample outlet. After the liquid suction head 31 of the pipette 30 is inserted into the bottle through the sample outlet 12, the gripping part 33 with the lid function is used to cover the sample outlet 12 to prevent the liquid in the bottle from flowing out of the sample outlet 12.

[0058] Figures 3 to 8 The shown sample collection device 1 includes a collection bottle 10, a sampler 20, and a pipette 30. The sample collection device also includes a sample inlet 11, a separating member 13, and a pipette support 14. A sample outlet 141 is provided on the pipette support 14. The separating member 13 with the sample inlet 11 is located at one end of the collection bottle, and the pipette support 14 with the sample outlet 141 is located at the other end of the collection bottle.

[0059] The structure of the separating member 13 and the sampler 20 and the cooperation relationship between them are the same as those of Figure 1 and Figure 2 the shown separating member and sampler. The sampling rod 22 of the sampler 20 is inserted into the collection bottle through the through hole 131 of the separating member 13 via the sample inlet 11, and the gripping part 23 of the sampler 20 covers the sample inlet 11.

[0060] The pipette support 14 used in cooperation with the pipette 30 includes a sample outlet 141 and a storage cavity 142. The pipette support 14 is installed at one end of the collection bottle 10. The sample outlet 141 communicates the inside of the collection bottle with the outside. One end of the storage cavity 142 is open, and the other end facing the inside of the collection bottle is closed.

[0061] As Figure 8 shown, the pipette support 14 can be installed on the collection bottle 10 by ultrasonic welding, and the separating member 13 and the collection bottle 10 can also be integrally formed.

[0062] The pipette 30 includes a liquid suction head 31, a holding part 33, and a stopper 34. The liquid suction head 31 and the stopper 34 are located on the holding part 33. The pipette may further include a connecting arm 32, and the liquid suction head 31 is connected to the holding part 33 through the connecting arm 32. The pipette 30 is connected to the pipette holder 14 in a plug-and-play manner, and the stopper 34 of the pipette is in interference fit with the sample outlet 141 of the pipette holder. Before sampling, the liquid suction head 31 of the pipette 30 is stored in the storage cavity 142, and the stopper 34 of the pipette is plugged into the sample outlet 141 of the pipette holder.

[0063] The liquid suction head 31 can be a capillary structure, with a liquid suction port 311 at its front end and a liquid outlet 312 at its rear end. There can be a certain space 313 above the liquid outlet to allow the gas in the liquid suction head to be discharged from the liquid outlet 312. After the capillary liquid suction port 311 comes into contact with the liquid, under the action of capillary siphon, the liquid will automatically be sucked into the capillary of the liquid suction head. The capillary can be a quantitative capillary of different specifications, such as a liquid suction volume of 1 μL, 5 μL, etc.

[0064] As Figure 1 shown, the sample collection device does not have a pipette holder 14, and the liquid suction head 31 of the pipette is directly inserted into the collection bottle body filled with liquid. Before the sampler 20 transfers the sample to the collection bottle, the liquid suction head 31 of the pipette extending into the collection bottle will come into contact with the liquid reagent in the collection bottle. When the liquid suction head is of a capillary structure, the reagent in the bottle will automatically be sucked into the capillary, that is, before sampling, there is already liquid in the liquid suction head 31. This may cause the liquid suction head 31 of the pipette to be unable to suck the mixed liquid for detection after the sample is mixed with the liquid, or it may be necessary to flip the sample collection device multiple times to allow the mixed liquid to be detected to enter the capillary liquid suction head 31.

[0065] As Figures 3 to 5 shown, the sample collection device is provided with a pipette holder 14. Before the sample is mixed with the reagent, the liquid suction head 31 of the pipette is stored in the storage cavity 142. Since one end of the storage cavity is sealed, the liquid suction head 31 is isolated from the reagent in the collection bottle. Therefore, before the sampler 20 transfers the sample to the collection bottle 10, the liquid suction head 31 of the pipette 30 will not come into contact with the liquid reagent in the collection bottle 10. After the sample is mixed with the reagent, the liquid suction head 31 of the pipette is taken out from the storage cavity 142. At this time, there is no liquid in the capillary of the liquid suction head 31 of the pipette. Therefore, when the liquid suction head 31 of the pipette is inserted into the sample outlet 141 to suck the mixed liquid in the collection bottle, after the liquid suction head is inserted from the sample outlet and comes into contact with the liquid, the capillary of the liquid suction head can quickly suck the mixed sample.

[0066] The opening sizes of the sample outlet 141 of the pipette holder 14 and the opening of the pipette tip storage cavity 142 can be different, so as to prevent the pipette tip and the stopper from being placed in the wrong position during assembly. For example, the opening of the sample outlet 141 is larger than the opening of the storage cavity 142. If the pipette 30 is inserted into the pipette holder in a way that the stopper 34 is paired with the sample outlet 141 during assembly, the pipette can be smoothly installed on the pipette holder; if the pipette is inserted into the pipette holder in a way that the stopper is paired with the storage cavity during assembly, it will be difficult for the stopper to be inserted into the storage cavity, so it can prompt people that the installation position may be incorrect.

[0067] In order to enable the sample collection device to be placed upright, the gripping parts of the sampler and the pipette can each have a horizontal plane, so that the sample collection device 1 can stand upright on the operating table surface regardless of which end is facing down.

[0068] The material of the sample collection device can be plastic or other materials.

[0069] Take Figure 5 、 Figure 6 、 Figure 7 The sample collection device 1 shown and Figure 15 and Figure 16 The calprotectin test card shown as an example to illustrate the usage method of the sample collection device.

[0070] The sample collection device 1 has been pre-loaded with the reagent 50, and the sampling rod 22 of the sampler is inserted into the through hole 131 to prevent the reagent in the collection bottle 10 from flowing out through the through hole 131. The stopper 34 of the pipette is inserted into the sample outlet 141 to prevent the reagent from flowing out of the sample outlet 12. The pipette tip 31 of the pipette is temporarily stored in the pipette tip storage cavity 142, and the pipette tip 31 of the pipette is a 5 μL capillary tube.

[0071] The test card 70 includes a sample addition hole 71, a diluent hole 72 is provided upstream of the sample addition hole, and an observation window 73 is downstream of the sample addition hole. A test strip is installed in the test card, such as a test strip based on the lateral flow principle.

[0072] Such as Figure 9 and Figure 10 shown, pull out the sampler 20 and insert it into the fecal sample 60 to take different parts of the fecal sample; as Figure 11 shown, re-insert the sampler 20 containing the sample back into the collection bottle 10 to mix the sample and the reagent; as Figure 12 、 Figure 13 and Figure 14 shown, turn the sample collection device 1 over so that the pipette 30 is on top; pull the pipette 30 off the sampler holder 14 and insert the pipette tip 31 into the sample inlet 141 of the sampler holder 14 to siphon 5 μL of the mixed sample in the collection bottle 10; as Figure 15 and Figure 16As shown, bring the liquid suction head 31 that has completed liquid suction into contact with the sample addition hole 71 of the test card 70. After the sample solution is adsorbed into the sample addition hole, the sample addition operation is completed.

[0073] After the sample addition is completed, add 80 μL of diluent to the diluent hole 72, check the result through the observation window 73, or read the test result through an instrument such as a fluorescence immunoassay analyzer.

[0074] Sealing experiment of the sample collection device in Example 1

[0075] Randomly select Figure 5 、 Figure 6 、 Figure 7 20 sample collection devices as shown. Experimental steps: Take out the sampler 20, pour diluent from the sample inlet 11 until the collection bottle is full, reinsert the sampler 20 and dry the outside of the sample collection device. Horizontally place these 20 sample collection devices filled with 6 ml of diluent into a transparent vacuum dryer (ASONE), and place a dry paper towel under each sample collection device. Open the transparent vacuum dryer, release the air until the negative pressure reaches 0.05 MPa, close the transparent vacuum dryer, and observe whether the dry paper towel is wet to determine whether there is a liquid leakage phenomenon in the sample collection device. The experimental results show that the sample collection device of the present invention does not show a liquid leakage phenomenon and has good sealing performance.

[0076] Liquid suction accuracy experiment of the sample collection device in Example 2

[0077] Randomly select Figure 5 、 Figure 6 、 Figure 7 10 sample collection devices as shown. Experimental steps: Take out the sampler 20, pour 6 ml of diluent from the sample inlet 11, and reinsert the sampler 20 to its original position. Insert the sampler 20 into the fecal sample for sampling, and then insert it into the collection bottle again through the sample inlet and the separation part, and shake well. Invert the sample collection device, pull out the sampler 30 of the sample collection device, insert the liquid suction head 31 of the sampler into the sample outlet 141 of the sampler bracket and suck the liquid (fecal mixture). Weigh the sampler once before and once after liquid suction to obtain the liquid suction amount of the sampler each time (liquid suction amount = weight of the sampler after sucking the sample - weight of the sampler before sucking the sample). The experimental results are shown in Table 1. From the experimental results, the liquid suction accuracy of the sampler is high.

[0078] Table 1

[0079]

[0080]

Claims

1. A sample collection device, comprising a collection bottle, a sampler and a separator. The opposite ends of the sample collection device are respectively provided with a sample inlet and a sample outlet end. It is characterized in that the separator is connected to the sample inlet in the collection bottle. The through hole of the separator is located below the sample inlet. The sampling rod of the sampler is inserted into the collection bottle through the through hole of the separator from the sample inlet. The sample collection device further comprises a sampler, which includes a liquid suction head and a holding part. The liquid suction head is set as a quantitative capillary tube, and the liquid suction head is inserted into the collection bottle from the sample outlet end.

2. The sample collection device according to claim 1, It is characterized in that a sampler support is provided at the sample outlet end of the sample collection device. The sampler support includes a sample outlet and a storage cavity. One end of the storage cavity is open, and the other end facing the inside of the collection bottle is closed.

3. The sample collection device according to claim 2, It is characterized in that the sampler further comprises a stopper. The liquid suction head of the sampler is located in the storage cavity, and the stopper of the sampler liquid-seals the sample outlet of the sampler support.

4. The sample collection device according to claim 3, It is characterized in that the stopper of the sampler and the sample outlet of the sampler support are in interference fit.

5. The sample collection device according to claim 3, It is characterized in that the opening sizes of the sample outlet of the sampler support and the opening of the liquid suction head storage cavity are different.

6. The sample collection device according to claim 2, It is characterized in that when sucking the sample in the collection bottle with the liquid suction head of the sampler, the liquid suction head of the sampler is inserted into the collection bottle from the sample outlet.

7. A sample detection method, It is characterized in that comprises the following steps: (1) Take out the sample collection device. The sample collection device includes a collection bottle, a sampler, a separator and a sampler. The opposite ends of the sample collection device are respectively provided with a sample inlet and a sample outlet end. The separator is connected to the sample inlet in the collection bottle. The through hole of the separator is located below the sample inlet. The sampler includes a liquid suction head and a holding part. The liquid suction head is set as a quantitative capillary tube; the sampler is installed on the sample inlet, and the sampler is installed at the sample outlet end; (2) Pull out the sampler from the sample inlet, and insert the sampling head of the sampler into the sample for sampling; (3) Reinsert the sampling rod containing the sample back into the collection bottle through the through hole of the separator, and mix the sample on the sampling head with the reagent in the bottle to form a mixed sample; (4) Flip the sample collection device with the sample outlet end facing up, and pull out the sampler sucking the mixed sample; (5) The sampler adds the mixed sample to the sample addition area of the test card; (6) The test card completes the detection of the sample.

8. The detection method according to claim 7, It is characterized in that a sampler support is provided at the sample outlet end of the sample collection device. The sampler support includes a sample outlet and a storage cavity. One end of the storage cavity is open, and the other end facing the inside of the collection bottle is closed.

9. The detection method according to claim 8, It is characterized in that the sampler further comprises a stopper; before forming the mixed sample, the liquid suction head of the sampler is stored in the storage cavity, and the stopper of the sampler is sealed in the sample outlet of the sampler support; after forming the mixed sample, separate the sampler from the sampler support, and insert the liquid suction head into the collection bottle through the sample outlet of the sampler support; The capillary of the pipette tip aspirates a quantified mixed sample.

10. The detection method according to claim 9, wherein, the plug of the sampler and the sample outlet of the sampler holder are in interference fit.

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