Atractylodes macrocephala koidz planting method

Abstract

The invention provides a bighead atractylodes rhizome planting method, and relates to the technical field of agricultural planting. The bighead atractylodes rhizome planting method comprises the following steps that bighead atractylodes rhizome is planted through a hole sowing method, fertilizer and soil are mixed and then applied at a time when the bighead atractylodes rhizome is planted, and a plant growth regulator is sprayed during planting. According to the method, the influence of combined application of the nitrogen phosphorus potassium fertilizer and the plant growth regulator on the growth of the atractylodes macrocephala koidz and mineral elements in soil is researched, and the optimal proportion beneficial to the growth of the atractylodes macrocephala koidz is screened out. 19 indexes of atractylodes macrocephala are comprehensively analyzed through a membership function, and the recommended appropriate fertilization amount for planting atractylodes macrocephala under the test conditions is 172 kg / hm < 2 > of nitrogen fertilizer (N), 180 kg / hm < 2 > of phosphorus fertilizer (P2O5), 156 kg / hm < 2 > of potassium fertilizer (K2O) and 100 mg / L of PP333, and growth of atractylodes macrocephala is facilitated.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural planting, and in particular to a method for planting Atractylodes macrocephala. Background Art

[0002] Atractylodes macrocephala Koidz. is a plant of the genus Atractylodes in the Asteraceae family. Its rhizome is used as medicine and it is a commonly used bulk Chinese herbal medicine variety. It is warm in nature, sweet and bitter in taste, and has the effects of strengthening the spleen and replenishing qi, drying dampness and promoting diuresis, stopping sweating, and calming the fetus. In recent years, with the excavation of wild Atractylodes resources, the number of wild Atractylodes has decreased sharply. Even in Zhejiang and Anhui, the amount of wild resources in the origin of Atractylodes is very small. Nowadays, Atractylodes production is mainly cultivated artificially, and artificial cultivation has problems such as unscientific fertilization, serious diseases and insect pests, and continuous cropping obstacles, which has led to a reduction in the planting area of ​​Atractylodes in many provinces where it has been introduced. The quality of seeds and Atractylodes cultivation seriously restricts the quality of Atractylodes medicinal materials. Fertilization can significantly improve the quality of seeds and Atractylodes cultivation. Paclobutrazol, as a growth retardant, can inhibit the vertical growth of plants, promote the lateral growth of plants, enhance the disease resistance and lodging resistance of plants, and thus achieve the purpose of increasing production. Paclobutrazol can be applied to Chinese medicinal materials (such as Angelica sinensis, Codonopsis pilosula, Cassia tora, etc.) and obtain good results. At present, the research on Atractylodes macrocephala is mainly focused on the separation of effective ingredients and their pharmacological effects. Although there are reports on fertilization technology, they are not systematic. Summary of the invention

[0003] Based on the technical problems existing in the background technology, the present invention proposes a method for planting Atractylodes macrocephala.

[0004] The invention provides a method for planting Atractylodes macrocephala, comprising the following steps: planting Atractylodes macrocephala by hole sowing, mixing fertilizer with soil and applying the fertilizer at one time when planting Atractylodes macrocephala, and spraying plant growth regulator during planting.

[0005] Preferably, the steps further include regular field management; the field management includes field weeding, etc.

[0006] Preferably, the fertilizer includes nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer.

[0007] More preferably, the amount of nitrogen fertilizer added is 100-175 kg / hm 2 , the amount of phosphate fertilizer added is 105~180kg / hm 2 , the amount of potassium fertilizer added is 70~235kg / hm 2 .

[0008] Preferably, the plant growth regulator is paclobutrazol.

[0009] The combined use of paclobutrazol and nitrogen, phosphorus and potassium fertilizers can promote the increase of various agronomic indicators of Atractylodes macrocephala, thereby providing a basis for the accumulation of biomass.

[0010] Preferably, the concentration of the plant growth regulator is 100-300 mg / L.

[0011] More preferably, the amount of nitrogen fertilizer added is 172 kg / hm 2 , the amount of phosphate fertilizer added is 180kg / hm 2 , the amount of potassium fertilizer added is 156kg / hm 2 ; The concentration of plant growth regulator is 100mg / L.

[0012] The beneficial effects of the present invention are:

[0013] The present invention adds nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer and plant growth regulator in the process of Atractylodes macrocephala planting, and the synergistic effect significantly increases the plant height, stem thickness, branch number, leaf number, flower bud number, root thickness, root length and biomass of Atractylodes macrocephala, and significantly increases the accumulation of soil mineral elements N, K, P, Mg and Na.

[0014] The present invention studies the effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on the growth of Atractylodes macrocephala and its effects on soil mineral elements, and screens out the best ratio that is beneficial to the growth of Atractylodes macrocephala. Through field experiments, a four-factor three-level orthogonal experimental design was used to determine the effects of different ratios of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on agronomic traits such as plant height, stem diameter, number of branches, number of leaves, and the accumulation of mineral elements in Atractylodes macrocephala. Results: Compared with the untreated group, N: 172kg / hm 2 , P 2 O 5 :180kg / hm 2 , K 2 O: 156kg / hm 2 PP 333 : 100mg / L significantly increased the plant height, stem diameter, number of branches, number of leaves, number of flower buds, root diameter, root length and biomass of Atractylodes macrocephala, and significantly increased the accumulation of soil mineral elements N, K, P, Mg, and Na, with increases of 216.19%, 136.33%, 195.77%, 174.44%, and 192.02%, respectively. Conclusion: Through the comprehensive analysis of 19 indicators of Atractylodes macrocephala through membership function, it is concluded that the recommended amount of nitrogen fertilizer (N) for planting Atractylodes macrocephala under the experimental conditions is: 172kg / hm 2、 Phosphate fertilizer (P 2 O 5 ): 180kg / hm 2 Potash fertilizer (K 2 O)156kg / hm 2 PP 333 : 100mg / L, is beneficial to the growth of Atractylodes macrocephala.

[0015] The invention adopts nitrogen, phosphorus and potassium fertilizers and a plant growth regulator paclobutrazol to plant Atractylodes macrocephala, and systematically explores the nitrogen, phosphorus and potassium fertilizers and the plant growth regulator paclobutrazol application ratio suitable for planting Atractylodes macrocephala by statistically analyzing the agronomic traits and mineral element accumulation of Atractylodes macrocephala under treatments with different ratios of nitrogen, phosphorus and potassium fertilizers and paclobutrazol, further improves the theoretical basis on which nitrogen, phosphorus and potassium fertilizers and the plant growth regulator paclobutrazol affect the production of Atractylodes macrocephala, and provides a theoretical reference for improving the yield and quality of Atractylodes macrocephala and medicinal materials. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on the leaf number of Atractylodes macrocephala.

[0017] Figure 2 To investigate the effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on the root growth of Atractylodes macrocephala.

[0018] Figure 3 To investigate the effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on the root diameter of Atractylodes macrocephala. DETAILED DESCRIPTION

[0019] The technical solution of the present invention is described in detail through specific embodiments.

[0020] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0021] Fertilizers used: nitrogen fertilizer (urea, N ≥ 46%, Guizhou Chitianhua Tongzi Chemical Co., Ltd.), potash fertilizer (agricultural potassium sulfate, K 2 O≥52.0%, State Investment Xinjiang Lop Nur Potash Co., Ltd.), phosphate fertilizer (general calcium, P 2 O 5 ≥12.0%, Guizhou Fuquan Chemical Co., Ltd.).

[0022] Test materials: Atractylodes rhizomes (cultivated Atractylodes macrocephala) were used as test materials. The materials were purchased from Ruihang Chinese Medicinal Materials Seedling Sales Company in Bozhou City, Anhui Province. The weight of each cultivated Atractylodes macrocephala was between 30 and 50 g. Plant growth regulator paclobutrazol (PP 333 ) was purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0023] 1. Experimental Design

[0024] This experiment adopted a four-factor three-level orthogonal experimental design, as shown in Table 1. Four factors were set, namely nitrogen fertilizer, phosphorus fertilizer, potassium fertilizer, and paclobutrazol, and each factor was set at three levels. N: 104 kg / hm 2 、138kg / hm 2 、172kg / hm 2 , P: 108kg / hm 2 、144kg / hm 2、180kg / hm 2 , K: 78kg / hm 2 、156kg / hm 2 、234kg / hm 2 , paclobutrazol: 100mg / L, 200mg / L, 300mg / L. Another untreated group T0 was set up as no fertilizer treatment, with a total of 10 treatments, each with 3 replicates. A total of 30 test plots were arranged in random blocks. The planting density of Atractylodes macrocephala was: row spacing: 35cm, plant spacing: 20cm, and the area of ​​each test plot was 10m 2 . The fertilizer was mixed with the soil and applied once when Atractylodes macrocephala was planted. The "hole sowing" method was used to plant Atractylodes macrocephala. During the period, each fertilization treatment group (except T0) in the nitrogen, phosphorus and potassium compound fertilizer test was sprayed with different concentrations of plant growth regulator paclobutrazol. In order to ensure the normal growth of Atractylodes macrocephala, field management such as field weeding was carried out regularly. The specific test plan is shown in Table 2.

[0025] Table 1

[0026]

[0027] Table 2

[0028]

[0029] 2. Measurement indicators and methods

[0030] 2.1 Basic physical and chemical properties of soil in the experimental site

[0031] The five-point sampling method was used to collect soil samples from the test site. The soil of 10-20 cm was dug at each point, impurities were removed, and the five points were mixed to serve as the basic fertility test samples of the site. The soil was air-dried, ground, and passed through 2.00 mm, 0.300 mm, and 0.149 mm sieves for the determination of basic physical and chemical properties, including total nitrogen, total phosphorus, total potassium, available phosphorus, available potassium, alkaline nitrogen, organic matter, and pH.

[0032] 2.2 Determination of agronomic traits of Atractylodes macrocephala

[0033] Random sampling was used, and each treatment and each plot (the planting density of Atractylodes macrocephala was: row spacing: 35 cm, plant spacing: 20 cm, and the area of ​​each experimental plot was 10m 2 . ) Randomly select three plants with good growth and no diseases as statistical objects, dig them out with roots, wash them, wipe them dry, and count their plant height, main stem thickness, root length, root thickness, number of leaves, number of flower buds, number of branches, root fresh weight, stem fresh weight, leaf fresh weight, root dry weight, stem dry weight, leaf dry weight (first kill the green at 105℃ and then dry at 55℃ to constant weight, after drying, the samples are divided into four parts of root, stem, leaf and bud according to the marks and stored for later use). The determination method is shown in Table 3.

[0034] Table 3

[0035]

[0036] 2.3 Determination of N, P, K, Na and Mg content in Atractylodes macrocephala

[0037] Take the dry sample powder treated in 2.2, accurately weigh 0.5g of the dry sample, and place it in a porcelain crucible. Use a muffle furnace to slowly incinerate at 200℃ for 2h, 400℃ for 2h, and 600℃ for 4h. Add 5ml of 50% nitric acid (HNO 3 :DIwater=1:1) to dissolve the ash, filter into a 25ml volumetric flask, make up to volume, and dilute 10 times. Use ICP (Inductively Coupled Plasma Emission Spectronmetry, ICP, Optima 8100) to determine the content of P, K, Na and Mg elements. Take the dry sample powder treated in 2.2, accurately weigh 0.5g of the dry sample, and use Kjeldahl nitrogen analyzer (FOSS 8400) to determine the content of N element.

[0038] 2.4 Determination of Atractylodes lactones Ⅰ, Ⅱ, Ⅲ and atractylodesone

[0039] ① Preparation of reference solution: Accurately weigh the reference substances respectively, make up to 25mL volumetric flask, and prepare the mother solutions of atractylodes lactone I, II, III and atractylodes ketone with concentrations of 0.7038mg / mL, 0.8231mg / mL, 0.7013mg / mL and 0.8532mg / mL respectively.

[0040] ② Preparation of sample solution: Accurately weigh 2 g of Atractylodes macrocephala powder (passed through No. 3 sieve), place in a stoppered test tube, add 20 mL of methanol, weigh, let stand for 30 min, ultrasonicate for 30 min, wait for cooling, add methanol to make up for the lost weight, filter, make up to 25 mL, filter through a 0.45 μm filter membrane, and set aside.

[0041] ③Standard curve: Accurately measure the mother solutions prepared in item ①, and dilute each mother solution to 0.00485 mg / mL, 0.0097 mg / mL, 0.0194 mg / mL, 0.0388 mg / mL, 0.05173 mg / mL, 0.07760 mg / mL for Atractylodes lactone I, and 0.00072 mg / mL, 0.00288 mg / mL, 0.00576 mg / mL for Atractylodes lactone II , 0.02157mg / mL, 0.02876mg / mL, atractylodes lactone Ⅲ were 0.07102mg / mL, 0.1776mg / mL, 0.3551mg / mL, 0.4735mg / mL, 0.7102mg / mL, atractylodes ketone were 0.221mg / mL, 0.2947mg / mL, 0.442mg / mL, 0.5892mg / mL, 0.8777mg / mL. The regression equation was obtained by taking the concentration of the reference solution as the abscissa and the chromatographic peak area as the ordinate. Atractylodes lactone Ⅰ: Y=6×10 7 X-7792(R 2 =0.9981) Atractylodes lactone II: Y = 9 × 10 7 X-7906(R 2 =0.9979) Atractylodes lactone III: Y = 4 × 10 7 X+34451(R 2 =0.9986) Atractylodes ketone: Y = 1 × 10 7 X-15607(R 2 =0.9991).

[0042] 2.5 Determination of moisture, total ash and extract of Atractylodes macrocephala

[0043] The moisture content of Atractylodes macrocephala is determined according to Method 2 of General Chapter 0832 of Part IV of the Pharmacopoeia of the People's Republic of China (2020 edition); the total ash content of Atractylodes macrocephala is determined according to General Chapter 2302; the extract of Atractylodes macrocephala is determined according to the hot soaking method under the determination method of alcohol-soluble extract (General Chapter 2201), using 60% ethanol as the solvent.

[0044] 2.6 Determination of Atractylodes macrocephala polysaccharides

[0045] ① Preparation of reference solution: Take 9.10 mg of anhydrous glucose reference substance dried to constant weight at 105°C, accurately weigh it, place it in a 25 ml volumetric flask, add water to dissolve and dilute to the scale, shake well, and obtain.

[0046] ② Preparation of standard curve: Accurately measure the reference solution, place it in a 10ml stoppered test tube, add water to 2.00ml, shake well, add 0.2% anthrone-sulfuric acid solution to the scale in an ice water bath, mix well, cool and then bathe in warm water for 10 minutes, take out, immediately place in an ice water bath for 10 minutes, take out, use the corresponding reagent as blank, and measure the absorbance at a wavelength of 582nm.

[0047] ③Sample preparation: Accurately weigh 0.25g of Atractylodes macrocephala sample, place in a 250ml conical flask, add 150ml of 80% ethanol, heat under reflux in a water bath for 1h, filter, retain the residue, add 150ml of water, heat under reflux in a water bath for 1h, cool, filter, take 1ml of filtrate, and measure the absorbance according to the method in item ② starting from "add 2.00ml of water", and calculate the polysaccharide content by the regression equation.

[0048] 2.7 Data Processing

[0049] Microsoft Excel 2010 software was used for raw data processing and chart drawing, SPSS22.0 statistical software was used for statistics and analysis, and One-way ANOVA was used for variance analysis (P<0.05).

[0050] Membership function = (X-Xmin) / (Xmax-Xmin); where X is the index measurement value, Xmin is the minimum value of the index measurement, and Xmax is the maximum value of the index measurement. The membership function values ​​of each index are accumulated and the average value is calculated. The larger the average value, the better the overall performance of the Atractylodes macrocephala plant.

[0051] 3. Results Analysis

[0052] 3.1 Effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on the growth of Atractylodes macrocephala

[0053] 3.1.1 Effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on the plant height of Atractylodes macrocephala

[0054] There are many factors that affect the production of Atractylodes macrocephala, such as germination conditions, soil fertility, climate change, cultivation measures, germplasm resources, etc. This study used fertilization and plant growth regulators together. The results are shown in Table 4. May to June is the period of rapid increase in plant height, and the plant height remains basically unchanged from July to November, indicating that the plant height of Atractylodes macrocephala has been established before July. In May, T7 and T9 treatments significantly increased the plant height of Atractylodes macrocephala seedlings. In June, T5, T6, T7 and T9 had a significant effect on the increase in plant height; compared with the control, the plant height of one-year-old Atractylodes macrocephala was significantly increased. From July to November, there was little effect on the plant height of Atractylodes macrocephala. It can be seen that the application of nitrogen fertilizer significantly increased the plant height of Atractylodes macrocephala, and the plant height increased with the increase in nitrogen application.

[0055] Table 4

[0056]

[0057]

[0058] Note: Different lowercase letters in the same column indicate significant differences (p<0.05), and the same lowercase letters indicate insignificant differences (p>0.05), the same below.

[0059] 3.1.2 Effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on stem diameter of Atractylodes macrocephala

[0060] As shown in Table 5, the stem diameter of Atractylodes macrocephala showed a gradual increase from May to November, from 0.92-2.99 mm in May to 5.11-8.23 mm in November; the increase in stem diameter was about 1.00 mm each month, except for September and October. Compared with the control, the stem diameter of annual Atractylodes macrocephala was significantly increased. From June to August, T9 had the best promoting effect on the increase of stem diameter, with increases of 103.03%, 62.60%, and 47.32% respectively compared with T0, and the promoting effect gradually decreased. It can be seen that the combined use of fertilization and plant growth regulators is conducive to the increase of stem diameter of Atractylodes macrocephala, but the effect is inconsistent in different periods.

[0061] Table 5

[0062]

[0063] 3.1.3 Effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on the number of branches of Atractylodes macrocephala

[0064] As shown in Table 6, the number of branches of Atractylodes macrocephala gradually increased from June to September, and the number of branches remained unchanged after September. There was no significant difference in the number of branches between the treatments from June to July, indicating that the apical dominance of the annual Atractylodes macrocephala was dominant at this time; in August, branch differentiation appeared, and the number of branches in each treatment was between 3.68 and 6.45; in September, there were differences in the number of branches in each treatment, and the number of branches was between 4.78 and 9.13; from October to November, the number of branches did not change significantly. August to September is the main period for the increase in the number of branches, that is, it appears in the reproductive growth period, and T9 has the best effect, with an increase of 75.27% and 67.52% respectively compared with the control group. It can be seen from the table that the combined use of fertilization and plant growth regulators significantly promotes the increase of Atractylodes macrocephala branches, but the effects of different ratio combinations are inconsistent.

[0065] Table 6

[0066]

[0067] 3.1.4 Effects of NPK fertilizers and plant growth regulators on the number of Atractylodes macrocephala leaves

[0068] like Figure 1As shown in the figure, from May to November, the number of Atractylodes macrocephala leaves under different treatments generally increased first and then decreased. The maximum number of leaves of T3, T5 and T9 appeared in October, the maximum number of leaves of T4 appeared in August, and the rest of the treatments were in September. The number of leaves in each treatment was relatively small in May; May to June was the first rapid increase period of leaf number, July to August was the second rapid increase period, June to July was the first slow increase period, and August to September was the second slow increase period. The number of leaves increased with the increase of nitrogen application, and nitrogen application significantly increased the number of leaves in the first and second rapid increase periods of leaves, and N2 and N3 could maintain the number of functional leaves.

[0069] 3.1.5 Effects of NPK fertilizers and plant growth regulators on root growth of Atractylodes macrocephala

[0070] like Figure 2 As shown in the figure, from May to November, the root length of Atractylodes macrocephala increased first and then decreased, and the maximum increase in each treatment was achieved in July. The root length increased rapidly from June to August. In May, fertilization and plant growth regulators had little effect on promoting root length growth. The root length of T0, T1, T2 and T3 treatments increased significantly in June, while the root length of T4, T5, T6, T7, T8 and T9 increased less, which may be due to the root's tendency to seek fertilizer. In July, the root length increase of T0, T1, T2 and T3 was less than that of T4, T5, T6, T7, T8 and T9. This period may be the period of normal elongation of the root length of Atractylodes macrocephala, so it is more conducive to root length growth under the condition of more fertilizer. The difference in the increase of root length of each treatment gradually narrowed in August; the increase of root length from September to November was not significant. In general, T1, T2, T4, T6, T7, T8 and T9 had better effects on promoting root length than the control, while the effects of T3 and T5 were not obvious.

[0071] 3.1.6 Effects of nitrogen, phosphorus, potassium fertilizers and plant growth regulators on root diameter of Atractylodes macrocephala

[0072] like Figure 3 As shown in the figure, the root diameter expansion of Atractylodes macrocephala under different treatments showed three periods, a slow expansion period from June to July, a rapid expansion period from August to October, and a slow expansion period in November, showing a slow-fast-slow trend. The application of fertilizers and plant growth regulators increased the increase in root diameter from August to October, with T3, T6, and T9 having the best effects.

[0073] 3.1.7 Effects of NPK fertilizers and plant growth regulators on the number of flower buds in Atractylodes macrocephala

[0074] As shown in Table 7, the number of flower buds of Atractylodes macrocephala was significantly increased under different treatments, and increased with the increase of nitrogen fertilizer. The number of flower buds under each treatment ranged from 3.56 to 12.98, and the increase in the treatment group compared with the control group was between 77.24% and 264.61%, and T7 had the largest number of flower buds.

[0075] Table 7

[0076]

[0077] 3.2 Effects of NPK fertilizers and plant growth regulators on the content of available N, P, K, Na and Mg in the soil around the roots of Atractylodes macrocephala

[0078] Mineral elements are the material basis for plant growth, which are crucial to the physiological and biochemical reactions of plants, and will affect the formation of crop yield and quality, and even affect the accumulation of important secondary metabolites. As shown in Table 8, the total accumulation of N, K, P, Mg and Na elements in Atractylodes macrocephala under different treatments was different. The combination of nitrogen, phosphorus and potassium fertilizers and paclobutrazol is conducive to the accumulation of N, K, P, Mg and Na elements in Atractylodes macrocephala, and the different proportions of nitrogen, phosphorus and potassium fertilizers and paclobutrazol will lead to differences in accumulation. Taking all factors into consideration, T9 can significantly promote the accumulation of N, K, P, Mg and Na elements in Atractylodes macrocephala, with increases of 216.19%, 136.33%, 195.77%, 174.44% and 192.02% respectively compared with T0.

[0079] In summary, the combined use of nitrogen, phosphorus and potassium fertilizers and plant growth agents can not only promote the increase of various agronomic indicators of Atractylodes macrocephala, but also maintain the duration of the leaf functional period, thereby providing a basis for the accumulation of biomass.

[0080] Table 8

[0081]

[0082] 3.3 Correlation analysis between yield and quality indexes of Atractylodes macrocephala under the combined application of nitrogen, phosphorus, potassium fertilizers and plant growth regulators

[0083] Table 9 shows the correlation analysis between the yield of Atractylodes macrocephala and various quality indicators of Atractylodes macrocephala under the application of nitrogen, phosphorus and potassium fertilizers and plant growth regulators. It can be seen that the water content of Atractylodes macrocephala is significantly negatively correlated with the yield, with a correlation coefficient of 0.89, and positively correlated with Atractylodes macrocephala lactone I, with a correlation coefficient of 0.79; the extract is significantly negatively correlated with polysaccharides, with a correlation coefficient of 0.86. The total ash is significantly positively correlated with the extract, with a correlation coefficient of 0.78; the yield is negatively correlated with ash, Atractylodes macrocephala lactone I, and Atractylodes macrocephala lactone II, with correlation coefficients of 0.14, 0.53, and 0.15, respectively, and positively correlated with polysaccharides, extracts, atractylodesone, and Atractylodes macrocephala lactone III, with correlation coefficients of 0.08, 0.03, 0.28, and 0.33, respectively.

[0084] Table 9

[0085]

[0086] 3.4 Membership function evaluation

[0087] As shown in Table 10, the membership function model was used to comprehensively analyze 19 indicators such as plant height, stem diameter, and number of branches in each treatment of Atractylodes macrocephala in the harvest month, and the membership function values ​​of all indicators were averaged. The result showed that the mean value of T9 treatment was the largest, that is, T9 treatment was the best ratio to promote the growth of Atractylodes macrocephala.

[0088] Table 10

[0089]

[0090]

[0091] In summary, the Atractylodes macrocephala planting method provided by the present invention provides an optimal fertilization ratio that is beneficial to the growth of Atractylodes macrocephala, significantly improves the plant height, stem thickness, number of branches, number of leaves, number of flower buds, root thickness, root length and biomass of Atractylodes macrocephala, and at the same time significantly increases the accumulation of soil mineral elements N, K, P, Mg and Na.

[0092] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A method for planting Atractylodes macrocephala, comprising the following steps: The hole sowing method is used to plant Atractylodes macrocephala. The fertilizer is mixed with the soil and applied all at once when the Atractylodes macrocephala is planted. Plant growth regulators are sprayed during the planting period.

2. The method for planting Atractylodes macrocephala according to claim 1, characterized in that: The fertilizers include nitrogen fertilizer, phosphorus fertilizer and potash fertilizer.

3. The method for planting Atractylodes macrocephala according to claim 2, characterized in that: The amount of nitrogen fertilizer added is 100-175 kg / hm 2 , the amount of phosphate fertilizer added is 105~180kg / hm 2 , the amount of potassium fertilizer added is 70~235kg / hm 2 .

4. The method for planting Atractylodes macrocephala according to claim 1, characterized in that: The plant growth regulator is paclobutrazol.

5. The method for planting Atractylodes macrocephala according to claim 1, characterized in that: The concentration of the plant growth regulator is 100-300 mg / L.

6. The method for planting Atractylodes macrocephala according to claim 1, characterized in that: The steps also include regular field management.

7. The method for planting Atractylodes macrocephala according to any one of claims 1 to 6, characterized in that: The amount of nitrogen fertilizer added is 172kg / hm 2 , the amount of phosphate fertilizer added is 180kg / hm 2 , the amount of potassium fertilizer added is 156kg / hm 2 ; The concentration of plant growth regulator is 100mg / L.

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