Grease composition for promoting sleep as well as preparation method and application thereof

Abstract

The invention provides a sleep-promoting grease composition as well as a preparation method and application thereof, and relates to the technical field of edible oil. The grease composition comprises, by weight, 40-50 parts of grease and 45-60 parts of auxiliary materials, the grease comprises rice oil, hemp seed oil and spina date seed oil according to the weight ratio of (20-30): (10-15): (5-10), and the auxiliary materials comprise a filler, an emulsifier and an antioxidant. According to the grease composition, the sleep rate, the sleep time and the GABA neurotransmitter level are improved, so that sedative hypnosis and sleep promotion can be achieved, meanwhile, the preparation method is simple, and the stability is good.

Description

Technical Field

[0001] The invention provides a sleep-promoting oil composition, a preparation method and application thereof, and relates to the technical field of edible oils. Background Art

[0002] At the neuronal level, specific areas in the brain, such as the hypothalamus, brainstem, and thalamus, control the sleep-wake cycle. These areas interact with neurotransmitters such as GABA and serotonin to promote or inhibit sleep. GABA is the most widespread neurotransmitter that promotes sleep, and the GABA-A receptor is a key target of the GABA signaling pathway. Serotonin (5-HT) is necessary for optimal sleep and wakefulness. Monoamine neurotransmitters such as norepinephrine promote wakefulness. Terpenes in oils and fats act on receptors and neurotransmitters, can interact with neurotransmitter receptors and increase the permeability of the blood-brain barrier, can relieve tension, improve cognitive function, and support sleep.

[0003] Rice oil is rich in oryzanol, tocopherol, phytosterols, etc. γ-oryzanol in rice oil exists in the form of steryl ferulate, which is a mixture of sterol and triterpene alcohol ferulates and is a strong antioxidant. Existing literature (doi:10.1038 / s41598-019-48743-8) records that after giving sleep disorder subjects 1000mg of rice bran extract (containing 4.5mg of γ-oryzanol) for two consecutive weeks, the subjects' sleep efficiency improved, total sleep time increased, and sleep latency decreased.

[0004] Hemp seed oil contains terpenes (β-caryophyllene, β-myrcene, limonene, linalool, etc.), phytosterols, tocopherols, etc. Terpenes are the source of the characteristic flavor substances of hemp seed oil. Existing literature (doi:10.3390 / molecules29092097) records that β-myrcene and linalool are active ingredients in hemp seed oil that promote sleep.

[0005] Jujube seed oil contains a variety of bioactive ingredients, such as terpenoids, phytosterols, and tocopherols. Existing literature (doi:10.1016 / j.heliyon.2024.e26979) records that terpenoids regulate sleep-related neurotransmitter signaling in the mouse brain, significantly increase 5-HT levels, enhance GABA synthesis, promote GABA-A receptor expression, and reduce glutamate and norepinephrine expression levels. Terpenoids have been identified as the active ingredients responsible for promoting sleep.

[0006] Chinese patent CN1491693A discloses the extraction of Chinese date seed oil and the preparation of soft capsules and the effect on the central nervous system. Chinese date seed oil has sedative, hypnotic, learning and memory promoting and anti-anxiety effects, and can be used to treat neurasthenia and insomnia. However, its effect needs to be improved and there is no research on the combination of Chinese date seed oil and other oils.

[0007] Chinese patent CN107616238A discloses a walnut oil for improving intelligence and nourishing the brain, which includes walnut oil, rice oil, linseed oil, rapeseed oil, perilla seed oil and grape seed oil; the additive includes one of hemp seed oil, sunflower kernel oil, pine nut oil, peanut oil or black sesame oil, and has the effect of improving intelligence and nourishing the brain. However, it does not conduct research on sedation and hypnosis. Summary of the invention

[0008] In order to solve the above technical problems, the present invention provides a sleep-promoting grease composition and a preparation method and application thereof. The grease composition can be sedative and hypnotic to promote sleep and has good stability.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0010] In a first aspect, the present invention provides a sleep-promoting oil composition, comprising, by weight: 40-50 parts of oil, 45-60 parts of auxiliary materials; the oil is rice oil, hemp seed oil and jujube seed oil in a weight ratio of (20-30):(10-15):(5-10); the auxiliary materials are fillers, emulsifiers and antioxidants.

[0011] Preferably, the sleep-promoting oil composition comprises, by weight, 50 parts of oil and 50 parts of auxiliary materials.

[0012] Preferably, the oil is rice oil, hemp seed oil and jujube seed oil in a weight ratio of (25-30):(10-15):10.

[0013] Further preferably, the oil is rice oil, hemp seed oil and wild jujube seed oil in a weight ratio of 25:15:10.

[0014] Furthermore, the auxiliary materials are fillers, emulsifiers and antioxidants in a weight ratio of (38-55):(6-11):(0.01-0.1).

[0015] Preferably, the auxiliary materials are fillers, emulsifiers and antioxidants in a weight ratio of (38-43):(7.5-11):(0.02-0.05).

[0016] Furthermore, the filler is at least one of resistant dextrin, resistant starch, maltodextrin, corn syrup and glucose syrup.

[0017] Furthermore, the emulsifier is emulsifier 1 and emulsifier 2 in a weight ratio of (5.5-10):(0.3-1).

[0018] Preferably, the emulsifier is emulsifier 1 and emulsifier 2 in a weight ratio of (7-10):(1-0.5).

[0019] More preferably, the emulsifier is emulsifier 1 and emulsifier 2 in a weight ratio of 7:0.5.

[0020] Furthermore, the emulsifier 1 is sodium caseinate.

[0021] Furthermore, the emulsifier 2 is at least one of mono- and di-glycerol fatty acid esters, acetylated mono- and di-glycerol fatty acid esters, and polyglycerol fatty acid esters.

[0022] Preferably, the emulsifier 2 is at least one of monoglycerol fatty acid ester, diglycerol fatty acid ester and polyglycerol fatty acid ester.

[0023] Furthermore, the antioxidant is vitamin E.

[0024] In a second aspect, the present invention provides a method for preparing a grease composition, comprising the following steps:

[0025] (1) heating and dissolving oil, antioxidant and emulsifier 2 to obtain L1;

[0026] (2) heating and dissolving the filler and emulsifier 1 to obtain L2;

[0027] (3) Mix L1 and L2, emulsify, filter, homogenize, sterilize, dry and then cool.

[0028] Furthermore, the heating temperature in step (1) and step (2) is 70-75°C.

[0029] Furthermore, the emulsification in step (3) is emulsification shearing, and the drying is spray drying.

[0030] Preferably, the filtration is double 80-100 mesh filtration.

[0031] Preferably, the homogenization is performed twice at 30-35 MP.

[0032] Preferably, the sterilization is UHT sterilization at 135°C for 5s.

[0033] Preferably, the drying temperature is 165-180°C.

[0034] In a third aspect, the present invention provides a product for promoting sleep, comprising the above-mentioned oil composition.

[0035] In a fourth aspect, the present invention provides a method for improving the stability of a fat composition, wherein the fat composition comprises, by weight, 40-50 parts of fat and 45-60 parts of auxiliary materials; the fat is rice oil, hemp seed oil and jujube seed oil in a weight ratio of (20-30):(10-15):(5-10); the auxiliary materials are fillers, emulsifiers and antioxidants.

[0036] The beneficial effects of the present invention are:

[0037] (1) The present invention uses a specific dosage relationship to make rice oil, hemp seed oil and jujube seed oil work synergistically, thereby improving the behavioral indicators and neurotransmitter levels of the sodium pentobarbital synergistic sleep experiment and improving the sedative and hypnotic effect of the oil composition of the present invention.

[0038] (2) The present invention improves the stability of the oil composition by compounding auxiliary materials of specific types and usage ratios, making it less likely to deteriorate during long-term storage and easier to store and transport.

[0039] (3) The preparation method of the present invention is simple, efficient and easy to produce on a large scale. DETAILED DESCRIPTION

[0040] In order to make the purpose, technical scheme and advantages of the present invention clearer, the present invention is described by the following specific examples, but is by no means limited thereto. The following is a preferred embodiment of the present invention, which is only used to describe the present invention and cannot be construed as a limitation of the present invention. It should be noted that any modification, equivalent replacement and improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The present invention does not limit the source of the raw materials used. If not otherwise specified, the raw materials used in the present invention are all common commercial products in the technical field, and Table 1 is an exemplary description.

[0041] Table 1

[0042]

[0043] 1. Examples and Comparative Examples

[0044] The material list of Examples 1-3 is shown in Table 2.

[0045] Table 2

[0046]

[0047] The material table of Comparative Examples 1-8 is shown in Table 3.

[0048] Table 3

[0049]

[0050]

[0051] The preparation methods of Examples 1-3 and Comparative Examples 1-8 are:

[0052] (1) heating oil, antioxidant and emulsifier 2 at 75° C. to dissolve to obtain L1;

[0053] (2) heating the filler and emulsifier 1 to 70° C. to dissolve to obtain L2;

[0054] (3) Mix L1 and L2, emulsify by shearing, filter through a double 80-100 mesh filter, homogenize twice with 35MP, sterilize at UHT 135°C for 5s, spray dry at 170°C, and cool to obtain the product.

[0055] 2. Effect Test

[0056] (1) Experimental Materials

[0057] 1) Experimental animals

[0058] 8-week-old SPF-grade ICR male mice, weighing 25±2 g, were provided by Beijing Weitonglihua Laboratory Animal Technology Co., Ltd. with license number: SYXK(Lu)20220009.

[0059] 2) Experimental reagents are shown in Table 4.

[0060] Table 4

[0061]

[0062] 3) Experimental instruments are shown in Table 5.

[0063] Table 5

[0064]

[0065] (2) Experimental methods

[0066] 1) Drug preparation

[0067] Intervention preparation: Example and comparative example 22500 mg + 75 mL normal saline, diazepam solution 6 tablets + 60 mL 0.5% sodium carboxymethylcellulose solution.

[0068] Preparation of sodium pentobarbital solution: subthreshold dose 40 mg / kg, 280 mg sodium pentobarbital + 70 mL normal saline; suprathreshold dose 65 mg / kg, 455 mg sodium pentobarbital + 70 mL normal saline.

[0069] After ultrasonic dissolution, divide into portions for later use.

[0070] 2) Animal handling and sampling

[0071] All mice were fed in an SPF environment, with free access to food and water, room temperature controlled at 20-24°C, relative humidity controlled at 40%-70%, and daily light-dark time ratio of 1:1, day and night cycle. The animal operations in this experiment all met the requirements of the Animal Protection Committee of Shandong University of Traditional Chinese Medicine (No. SDUTCM20230327002) on experimental animal operations and animal welfare. After three days of adaptive feeding, the mice were randomly divided into 9 groups, 10 in each group, with normal saline, diazepam solution, Example 1, Example 2, Comparative Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5. The mice were gavaged for 14 consecutive days. The experimental group used Example 1 (3000 mg / kg) and Example 2 (3000 mg / kg) as emulsions, the control group used Comparative Examples 1-5 (3000 mg / kg) as emulsions, the positive control group used diazepam solution (2.5 mg / kg), the negative control group used normal saline (10 ml / kg), and the sodium pentobarbital solution was injected intraperitoneally. The whole brain was quick-frozen in liquid nitrogen.

[0072] 3) Detection indicators

[0073] ①Weight test: Measure your weight 14 days later.

[0074] ② Sleeping rate experiment: mice were intraperitoneally injected with 40mg / kg sodium pentobarbital solution, and observed whether they fell asleep within 30 minutes, and the sleeping rate was calculated. The criterion for judging whether the animal fell asleep was the disappearance of the turning reflex, that is, after the injection of sodium pentobarbital solution, the animal was turned over when it was motionless. If the mouse was in the supine position for 1 minute, it was considered to be asleep.

[0075] ③ Induced sleep experiment: Mice were intraperitoneally injected with 65mg / kg sodium pentobarbital solution, and the changes in the mice's sleep latency and sleep time were observed. The sleep latency is the time from the injection of sodium pentobarbital solution to the disappearance of the flipping reflex, and the sleep time is the time from the mice falling asleep to waking up. The criterion for judging the awakening of mice is the recovery of the righting reflex. When the mouse is sleeping (the righting reflex disappears), it is in the supine position. When it returns to the normal position, it is immediately turned over to the supine position. If it recovers again within 30s, it is judged to be awake.

[0076] ④ ELISA method was used to determine the contents of GABA, GABAAR, 5-HT, IL-1β, and NE according to the instructions. GABA (γ-aminobutyric acid) is the main inhibitory neurotransmitter, which promotes relaxation and sleep by reducing the excitability of neurons. GABAAR (GABAA type receptor) is the main receptor for GABA, which reduces neural activity by regulating chloride ion channels, exerting a sedative and sleep effect. Norepinephrine (NE) increases alertness and wakefulness by enhancing the activity of the sympathetic nervous system. Serotonin (5-HT) has a dual effect of supporting wakefulness during the day and promoting sleep at night by converting to melatonin. Interleukin-1β (IL-1β), as a pro-inflammatory factor, helps regulate sleep at appropriate levels, but excessive amounts may trigger an inflammatory response.

[0077] 4) Statistical methods

[0078] All data in this study were double-entered and analyzed using IBM SPSS28.0 software package. Measurement data were described in the form of (mean±SD). Normally distributed data were compared using variance analysis, and non-normally distributed data were compared using rank sum test. P≤0.05 indicated statistical significance.

[0079] 5) Experimental results

[0080] The results of body weight and behavioral indicators of the sodium pentobarbital-assisted sleep experiment in each group are shown in Table 6.

[0081] Table 6

[0082]

[0083] Compared with the normal saline group, a: P < 0.05. Compared with Example 1, b: P < 0.05.

[0084] As shown in Table 6, compared with the saline group (negative control), the diazepam liquid group (positive control) mice had significant differences in body weight, and there were no statistical differences in other groups. The results of the subthreshold dose hypnosis experiment of sodium pentobarbital showed that the diazepam liquid group (positive control) and the Example 1 group performed well in terms of the rate of falling asleep, and the Example 2 group was second. The sodium pentobarbital suprathreshold dose hypnosis experiment found that compared with the saline group (negative control), there was no statistical difference in the latency of falling asleep in each group, and the Example 1 group had a slight advantage; in terms of sleep time, compared with the saline group (negative control), there were significant differences between the Example 1 group and the Example 2 group, and there were no statistical differences in other groups. The sleep time of Comparative Examples 1-5 was significantly lower than that of Examples 1-2, and Comparative Examples 2, 3, and 5 were significantly different from Example 1.

[0085] The neurotransmitter levels in each group are shown in Table 7.

[0086] Table 7

[0087]

[0088] Compared with the normal saline group, a: P < 0.05. Compared with Example 1, b: P < 0.05.

[0089] As shown in Table 7, compared with the saline group (negative control), there are significant differences in GABA content between Example 1 and Example 2, and no statistical differences in other groups. The GABA content of Comparative Examples 1-5 is significantly lower than that of Examples 1-2, and is significantly different from that of Example 1.

[0090] 3. Stability Test

[0091] (1) Experimental Materials

[0092] 1) Experimental reagents are shown in Table 8.

[0093] Table 8

[0094]

[0095]

[0096] 2) Experimental instruments are shown in Table 9.

[0097] Table 9

[0098]

[0099] (2) Experimental methods

[0100] 1) Embedding rate = 1-surface oil content / total oil content

[0101] 2 g of Example 1 and Comparative Examples 6-8 were placed in a Buchner funnel, washed with 25 mL of petroleum ether, and filtered. The filtrate was collected and heated.

[0102] Drying can be used to obtain the surface oil content; Soxhlet extraction method can be used to measure the total oil content.

[0103] 2) Changes in peroxide value (POV) during accelerated storage experiment

[0104] POV is the main indicator for evaluating whether oxidative rancidity occurs in oil products. Accelerated oxidation experiments were carried out on the embodiments and comparative examples. 100 g of the sample was placed in a 60°C incubator for 6 days of accelerated oxidation experiment. The POV value was tested at 0, 2, 4, and 6 days. The POV value test method refers to GB 5009.227-2023.

[0105] (3) Experimental results

[0106] The embedding rate and POV value of each group are shown in Table 10.

[0107] Table 10

[0108]

[0109]

[0110] As the amount of sodium caseinate decreased (7%→5%→2%), the amount of mono- and di-glycerol fatty acid esters decreased (0.3%→0.1%), and the amount of antioxidants decreased (0.02%→0%), the surface oil content of the oil powder increased, the embedding rate decreased, and the embedding effect was poor. Under accelerated storage conditions, the peroxide values ​​of Example 1, Comparative Example 6, Comparative Example 7, and Comparative Example 8 showed a gradual increase. By comparison, it was found that Comparative Example 8 was the most susceptible to oxidation and rancidity, followed by Comparative Example 7, and Example 1 was the least susceptible to oxidation and rancidity.

[0111] The present invention is described in detail above. The present invention uses specific examples to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the present invention and its core ideas. It should be pointed out that for ordinary technicians in this technical field, without departing from the principles of the present invention, the present invention can also be improved and modified in a number of ways, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A sleep-promoting fat composition, characterized in that: In parts by weight, it includes: 40-50 parts of fat, 45-60 parts of auxiliary materials; The oil is rice oil, hemp seed oil and jujube seed oil in a weight ratio of (20-30):(10-15):(5-10); The auxiliary materials are fillers, emulsifiers and antioxidants.

2. The grease composition according to claim 1, characterized in that The auxiliary materials are fillers, emulsifiers and antioxidants in a weight ratio of (38-55):(6-11):(0.01-0.1).

3. The grease composition according to claim 2, characterized in that The filler is at least one of resistant dextrin, resistant starch, maltodextrin, corn syrup and glucose syrup; and / or The emulsifiers are emulsifier 1 and emulsifier 2 in a weight ratio of (5.5-10):(0.3-1); and / or The emulsifier 1 is sodium caseinate; and / or The emulsifier 2 is at least one of mono- and di-glycerol fatty acid esters, acetylated mono- and di-glycerol fatty acid esters and polyglycerol fatty acid esters; and / or The antioxidant is vitamin E.

4. The method for preparing the grease composition according to any one of claims 1 to 3, characterized in that: The steps include: (1) heating and dissolving oil, antioxidant and emulsifier 2 to obtain L1; (2) heating and dissolving the filler and emulsifier 1 to obtain L2; (3) Mix L1 and L2, emulsify, filter, homogenize, sterilize, dry and then cool.

5. The preparation method according to claim 4, characterized in that: The heating temperature in step (1) and step (2) is 70-75°C.

6. The preparation method according to claim 4, characterized in that: The emulsification in step (3) is emulsification shearing, and the drying is spray drying.

7. A product for promoting sleep, characterized in that: The invention comprises the grease composition according to any one of claims 1 to 3 or the grease composition prepared by the preparation scheme according to any one of claims 4 to 6.

8. The product according to claim 7, characterized in that The product is a food.

9. Use of the oil composition according to any one of claims 1 to 3 or the oil composition prepared according to any one of claims 4 to 6 in the preparation of sedative and hypnotic products.

10. A method for improving the stability of a grease composition, characterized in that: The oil composition comprises, by weight: 40-50 parts of oil and 45-60 parts of auxiliary materials; The oil is rice oil, hemp seed oil and jujube seed oil in a weight ratio of (20-30):(10-15):(5-10); The auxiliary materials are fillers, emulsifiers and antioxidants.

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