Fermented Chinese herbal medicine preparation for increasing lactation yield of sows and preparation method of fermented Chinese herbal medicine preparation

Abstract

The invention discloses a fermented Chinese herbal medicine preparation for increasing the lactation yield of sows and a preparation method of the fermented Chinese herbal medicine preparation, Chinese herbal medicines mainly comprise motherwort, folium artemisiae argyi, radix isatidis and jujube powder, and fermented probiotics mainly comprise bacillus subtilis, bacillus coagulans, enterococcus faecalis, lactobacillus plantarum and saccharomycetes. The fermented traditional Chinese medicine preparation produced by the invention has the effects of improving intestinal health and immunity and improving the antioxidant and anti-stress effects of livestock and poultry in the production of the lactating sows, so that the lactation performance of the lactating sows and the production performance of piglets are improved.

Description

Technical Field

[0001] The invention relates to the technical field of fermented Chinese herbal medicine preparation, and in particular to a fermented Chinese herbal medicine preparation for improving sow lactation and a preparation method thereof. Background Art

[0002] Over the past few decades, as the breeding process has accelerated, the litter size of sows has continued to increase from 10 to 20 pigs. Nipple competition has occurred in the litter size and may lead to increased piglet mortality. It has been observed that piglets from large litters spend more time stimulating the udder, causing them to lose more energy and produce less milk; the amount of milk obtained by piglets may be affected by factors such as the number of suckling piglets, the size and health of the piglets, and the stage of lactation. Therefore, as the litter size increases, how to improve the lactation ability of sows has become a research focus and hotspot. Studies have found that drug-induced lactation may have negative effects, while Chinese herbal lactation agents are becoming increasingly popular. Summary of the invention

[0003] The purpose of the present invention is to overcome the above technical deficiencies, propose a fermented Chinese herbal medicine preparation for increasing sow lactation and a preparation method thereof, and solve the technical problems of low effective drug utilization and poor effect of drugs in the prior art.

[0004] In order to achieve the above technical purpose, the first aspect of the present invention provides a fermented Chinese herbal medicine preparation for increasing the lactation of sows, wherein the fermentation matrix and the fermentation agent are in a ratio of 1:1. According to the percentage of added amount, the compatibility and content of the fermentation matrix (Chinese herbal medicine and other nutrients) are as follows: 12% corn, 5% wheat bran, 14.7% soybean meal, 25% shrimp shell powder, 1% benzoic acid, 0.3% sodium butyrate, 15% motherwort, 8% wormwood, 11% isatis root, 8% jujube powder; the proportion and content of the fermentation agent are as follows: 1% Bacillus subtilis, 0.6% Bacillus coagulans, 1.2% Enterococcus faecalis, 0.8% Lactobacillus plantarum, 0.9% yeast, 5.5% brown sugar, and 90% purified water.

[0005] The second aspect of the present invention provides a method for preparing a fermented Chinese herbal medicine preparation for increasing sow lactation, comprising the following steps:

[0006] The probiotic solution is prepared by mixing fermentation bacteria such as Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis, Lactobacillus plantarum, yeast, brown sugar and purified water, stirring evenly, and standing at 36° C. for 24 hours.

[0007] Corn, wheat bran, soybean meal, shrimp shell powder, benzoic acid, sodium butyrate, motherwort, wormwood, isatis root, and jujube powder are added to the probiotic solution and stirred evenly to make the moisture content of the fermented Chinese herbal medicine reach 42%. Anaerobic fermentation is carried out at 36° C. for 10 hours to obtain a fermented Chinese herbal medicine preparation for increasing sow lactation.

[0008] The method for preparing the fermented Chinese herbal medicine preparation for increasing sow lactation provided in the second aspect of the present invention is used to prepare the fermented Chinese herbal medicine preparation for increasing sow lactation provided in the first aspect of the present invention.

[0009] Compared with the prior art, the present invention has the following beneficial effects:

[0010] The fermented Chinese herbal medicine preparation for increasing sow lactation provided by the present invention enhances the efficacy of the drug and reduces toxic side effects through the mutual synergy of traditional Chinese herbal medicine and probiotics, and is used to improve the lactation performance of sows. It has the characteristics of improving nutrient utilization, increasing bioactive ingredients, improving intestinal health and immunity, anti-oxidation and anti-stress, and improving palatability and feed intake. DETAILED DESCRIPTION

[0011] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0012] The first aspect of the present invention provides a fermented Chinese herbal medicine preparation for increasing the lactation of sows, wherein the fermentation substrate and the fermentation agent are in a ratio of 1:1. According to the percentage of added amount, the compatibility and content of the fermentation substrate (Chinese herbal medicine and other nutrients) are as follows: 12% corn, 5% wheat bran, 14.7% soybean meal, 25% shrimp shell powder, 1% benzoic acid, 0.3% sodium butyrate, 15% motherwort, 8% wormwood, 11% isatis root, 8% jujube powder; the proportion and content of the fermentation agent are as follows: 1% Bacillus subtilis, 0.6% Bacillus coagulans, 1.2% Enterococcus faecalis, 0.8% Lactobacillus plantarum, 0.9% yeast, 5.5% brown sugar, and 90% purified water.

[0013] The main active ingredients of motherwort are flavonoids, alkaloids, diterpenes, phenylethanoid glycosides, etc., which have the functions of regulating the uterus, enhancing immune function, resisting inflammation, and improving antioxidant levels. Flavonoids have antioxidant and estrogen-like functions, and have the functions of promoting postpartum uterine recovery and regulating endocrine; alkaloids have the functions of promoting blood circulation, regulating menstruation, and contracting the uterus, and are often used to stimulate rhythmic uterine contractions and excite the uterus; phenylethanoid glycoside compounds are a natural product with a wide range of pharmacological activities such as antibacterial, anti-inflammatory, antiviral, and antioxidant.

[0014] Artemisia argyi is the leaf of wormwood, which contains flavonoids, volatile oils, polysaccharides, terpenes, tannins, eucalyptone, trace elements and other ingredients. Traditional Chinese medicine believes that it has the effects of warming the blood and meridians, and is often used to treat symptoms such as uterine bleeding and menstrual disorders caused by deficiency and cold. Modern medical research has found that the ingredients of wormwood have multiple pharmacological effects such as anti-inflammatory, anti-tumor, antioxidant, antibacterial, antiviral, and immune regulation. In animal production, it can be used as a feed additive to promote animal growth and development and improve the quality of livestock and poultry products.

[0015] Isatis root is the dried root of the cruciferous plant Isatis indigotica. Its effective active ingredients mainly include nucleosides, lignans, amino acids, alkaloids, organic acids, polysaccharides, etc. It has antibacterial and anti-inflammatory, antiviral, anti-endotoxin and immunomodulatory effects.

[0016] Jujube powder is made from high-quality red dates through grinding, which retains most of the nutrients and medicinal value of red dates. Jujube powder is rich in nutrients, such as polysaccharides, vitamins, minerals, etc. These ingredients may indirectly enhance the efficacy of other Chinese herbal medicines by enhancing the body's immunity, improving microcirculation, and promoting drug absorption.

[0017] From the above description, it can be seen that the beneficial effects of the present invention are: the traditional Chinese herbal medicine made of motherwort, wormwood, isatis root, and jujube powder and probiotics exert efficient synergistic effects, enhance the efficacy of drugs, reduce toxic side effects, improve the utilization rate of nutrients, increase bioactive ingredients, improve intestinal health and immunity, anti-oxidation and anti-stress, and improve palatability and feed intake, and can be effectively used to increase sow milk production.

[0018] Preferably, the mass ratio of the total amount of corn, wheat bran, soybean meal, shrimp shell powder, benzoic acid, sodium butyrate to the total amount of motherwort, wormwood, isatis root, and jujube powder is 1: (0.7-0.8). Within this range, it can provide energy and nutrition, improve feed quality, improve animal intestinal environment, promote animal digestion and absorption, improve feed utilization, and have a certain antibacterial effect.

[0019] Preferably, the fermented Chinese herbal medicine preparation for increasing sow lactation, the fermentation matrix and the fermentation agent, the ratio is 1:1. According to the percentage of added amount, the compatibility and content of the fermentation matrix (Chinese herbal medicine and other nutrients) are: corn 12%, wheat bran 5%, soybean meal 14.7%, shrimp shell powder 25%, benzoic acid 1%, sodium butyrate 0.3%, motherwort 15%, wormwood 8%, isatis root 11%, jujube powder 8%; the fermentation agent ratio and content are: Bacillus subtilis 1%, Bacillus coagulans 0.6%, Enterococcus faecalis 1.2%, Lactobacillus plantarum 0.8%, yeast 0.9%, brown sugar 5.5%, purified water 90%.

[0020] The second aspect of the present invention provides a method for preparing a fermented Chinese herbal medicine preparation for increasing sow lactation, comprising the following steps:

[0021] S1: Mix Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis, Lactobacillus plantarum, yeast and other fermentation agents, brown sugar and purified water, stir evenly, and let stand at 36°C for 24 hours to prepare a probiotic solution.

[0022] S2: Add corn, wheat bran, soybean meal, shrimp shell powder, benzoic acid, sodium butyrate, motherwort, wormwood, isatis root, and jujube powder to the above probiotic solution, stir evenly to make the moisture content of the fermented Chinese herbal medicine reach 42%, and perform anaerobically fermentation at 36°C for 10 hours to obtain a fermented Chinese herbal medicine preparation for increasing sow lactation.

[0023] Preferably, the raw material powders of motherwort, wormwood, isatis root and red dates are all obtained by drying, crushing and sieving.

[0024] Preferably, the raw material powders of motherwort, wormwood, isatis root and red dates are obtained by the following steps:

[0025] Specifically, the raw material powder is obtained by drying, crushing and sieving motherwort, wormwood, isatis root and red dates, respectively, wherein the drying temperature is 40-60° C., the drying time is 6-12 hours, and the particle size of the obtained raw material powder passes through a 100-mesh sieve.

[0026] To avoid redundancy, in the following embodiments of the present invention, the raw material powders of motherwort, wormwood, isatis root and red dates are obtained by the following steps:

[0027] The raw material powder (motherwort, wormwood, isatis root, red dates) was air-dried at 60° C. for 6 hours and passed through a 100-mesh sieve to obtain raw material powder.

[0028] It should be noted that since the extraction rates of motherwort, wormwood, isatis root and red dates are inconsistent, in the process of preparing the raw material powders of motherwort, wormwood, isatis root and red dates, motherwort, wormwood, isatis root and red dates are extracted separately through the above-mentioned process, and then the obtained extracts are mixed according to the amount for use in the present invention.

[0029] It should be noted that in the following embodiments, the Chinese medicine components are: motherwort, wormwood, isatis root, jujube powder, the required components are corn, wheat bran, soybean meal, shrimp shell powder, benzoic acid, sodium butyrate, and the other components are Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis, Lactobacillus plantarum, yeast, brown sugar, and purified water.

[0030] Test Case

[0031] The fermented Chinese herbal medicine produced by the present invention is used to carry out animal experiments.

[0032] 45 "Chang × Da" binary hybrid sows with 3 to 4 parities, good health and similar weight, one week before delivery, were selected and randomly divided into three treatment groups. The three groups of sows were fed with the fermented Chinese herbal medicine of Example 1 of the invention for testing, including the control group (basic diet), the 2% fermented Chinese herbal medicine group (basic diet + 2% fermented Chinese herbal medicine preparation), and the 4% fermented Leonurus group (basic diet + 4% fermented Chinese herbal medicine preparation). There were 15 replicates in each group, and 1 sow in each replicate. All sows were transferred to the delivery room one week before the due date, and were raised in the delivery pen during the lactation period. They were fed freely and supplied with water by automatic drinking fountains. The pig house was kept ventilated, hygienic and dry. The pre-test period was 7 days, and the main test period was 21 days. The colostrum of the sows was collected within 4 hours after delivery. 30 mL of colostrum samples were manually collected from the nipples in the front, middle and back of the breast, and the colostrum-related indicators were measured to analyze the use effect. Evaluation criteria: total lactation capacity, daily lactation capacity, milk fat rate, milk protein rate, lactose rate, non-fat milk solids, and density. The test results are shown in Table 1.

[0033] Table 1

[0034] project Control group 2% Fermented Chinese Herbal Medicine Group 4% Fermented Chinese Herbal Medicine Group Total lactation capacity / L 140.080 152.200 178.780 Daily lactation capacity / L 6.667 7.240 8.520 Milk fat percentage / (%) 3.900 4.000 4.700 Milk protein rate / (%) 7.700 7.900 8.300 Lactose rate / (%) 11.900 12.000 12.500 Non-fat milk solids / (%) 21.300 21.600 22.300 Density / (kg / m3) 71.125 75.650 77.675

[0035] As can be seen from Table 1, Example 1 of the present invention has a good effect on increasing sow lactation. Compared with no addition of fermented Chinese herbal preparation, adding 2% and 4% of fermented Chinese herbal preparation to feed can increase total lactation capacity, daily lactation capacity, milk fat rate, and milk protein rate.

[0036] The fermented Chinese herbal medicine preparation of the present invention can be used to improve the lactation performance of sows, and has the characteristics of regulating endocrine, improving mammary tissue function or promoting the absorption and conversion of nutrients, thereby increasing milk secretion and improving the quality of milk components.

[0037] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A fermented Chinese herbal medicine preparation for increasing sow lactation, comprising a fermentation matrix and a fermentation agent in a ratio of 1:

1. Calculated by percentage of added amount, the fermentation matrix (Chinese herbal medicine and other nutrients) is composed of: 12% corn, 5% wheat bran, 14.7% soybean meal, 25% shrimp shell powder, 1% benzoic acid, 0.3% sodium butyrate, 15% motherwort, 8% wormwood leaf, 11% isatis root, 8% jujube powder; the fermentation agent is composed of: 1% Bacillus subtilis, 0.6% Bacillus coagulans, 1.2% Enterococcus faecalis, 0.8% Lactobacillus plantarum, 0.9% yeast, 5.5% brown sugar, and 90% purified water.

2. According to claim 1, the fermented Chinese herbal medicine preparation for increasing sow lactation, the fermentation matrix (Chinese herbal medicine and other nutrients), the mass ratio of the total amount of corn, wheat bran, soybean meal, shrimp shell powder, benzoic acid, sodium butyrate added to the total amount of motherwort, wormwood, isatis root, and jujube powder added is 1: (0.7~0.8).

3. According to claim 1, the fermented Chinese herbal medicine preparation for increasing sow lactation, the fermentation bacteria, the total amount of Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis, Lactobacillus plantarum, yeast added to the mass ratio of brown sugar and purified water is 1:1.2:

20.

4. The fermented Chinese herbal medicine preparation for increasing sow lactation according to claim 1, wherein the ratio of the fermentation substrate to the fermentation agent is 1:

1. According to the percentage of addition, the compatibility and content of the fermentation substrate (Chinese herbal medicine and other nutrients) are: corn 12%, wheat bran 5%, soybean meal 14.7%, shrimp shell powder 25%, benzoic acid 1%, sodium butyrate 0.3%, motherwort 15%, wormwood 8%, isatis root 11%, jujube powder 8%; the ratio and content of the fermentation agent are: Bacillus subtilis 1%, Bacillus coagulans 0.6%, Enterococcus faecalis 1.2%, Lactobacillus plantarum 0.8%, yeast 0.9%, brown sugar 5.5%, purified water 90%.

5. A method for preparing a fermented Chinese herbal medicine preparation for increasing sow lactation as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: The probiotic solution is prepared by mixing fermentation bacteria such as Bacillus subtilis, Bacillus coagulans, Enterococcus faecalis, Lactobacillus plantarum, yeast, brown sugar and purified water, stirring evenly, and standing at 36° C. for 24 hours. Corn, wheat bran, soybean meal, shrimp shell powder, benzoic acid, sodium butyrate, motherwort, wormwood, isatis root, and jujube powder are added to the probiotic solution and stirred evenly to make the moisture content of the fermented Chinese herbal medicine reach 42%. Anaerobic fermentation is carried out at 36° C. for 10 hours to obtain a fermented Chinese herbal medicine preparation for increasing sow lactation.

6. The method for preparing the fermented Chinese herbal medicine preparation for increasing sow lactation according to claim 5, characterized in that: The raw material powders of motherwort, wormwood leaf, isatis root and red dates are all obtained by drying, crushing and sieving.

7. The method for preparing the fermented Chinese herbal medicine preparation for increasing sow lactation according to claim 6, characterized in that: The raw material powders of motherwort, wormwood, isatis root and red dates are obtained by the following steps: Cleaning: Choose fresh motherwort plants, wormwood plants, and isatis root plants, clean them, and remove dirt, dead leaves, and other debris. Remove the pits from the red dates and wash them. dry: After washing, low-temperature drying equipment is used to accelerate the drying process to ensure that the internal moisture of motherwort, wormwood, isatis root and red dates is reduced to a level suitable for grinding. Crushing: The dried motherwort, wormwood leaf, isatis root and red dates are first cut into small pieces or fragments to make them easier to crush. Then a Chinese medicine grinder is used to grind the chopped motherwort, wormwood leaf, isatis root and red dates into powder. Sieve: The crushed motherwort, wormwood, isatis root and red dates powders are gently shaken through a sieve to allow fine powder to pass through the sieve holes, leaving larger particles or lumps for re-crushing until a satisfactory powder fineness is obtained.

8. The method for preparing the fermented Chinese herbal medicine preparation for increasing sow lactation according to claim 7, characterized in that: The raw material powder is obtained by drying, crushing and sieving motherwort, wormwood, isatis root and red dates respectively.

9. The method for preparing the fermented Chinese herbal medicine preparation for increasing sow lactation according to claim 8, characterized in that: The drying temperature is 40-60° C., the drying time is 6-12 hours, and the particle size of the obtained raw material powder passes through a 100-mesh sieve.