Children complementary food nutritional supplement helpful for removing lead

Abstract

The invention discloses a formula and a preparation method of a children complementary food nutritional supplement helpful for removing lead, the children complementary food nutritional supplement takes whole milk powder and instant bean flour as main raw materials, and seven vitamins, four minerals, five medicinal and edible materials, three dietary fibers and other ingredients are added to prepare the children complementary food nutritional supplement helpful for removing lead. The material is prepared by a material expansion pulse circulation type mixing technology, and is filled with composite inert gases (nitrogen and carbon dioxide) according to a certain proportion. According to the present invention, the nutrients and the dietary fibers are matched, and the edible and medicinal materials containing a variety of natural active components are matched so as to chelate and capture the metal lead in the children after the children eat, such that the discharge of the metal lead is promoted, the accumulation of the lead in the blood and the tissue is reduced, and the injury caused by lead poisoning is reduced. A high-lead rat model intervention experiment and observation and comparison of the test effect of voluntary tested children with lead exposure contact history and high blood lead content prove that the traditional Chinese medicine composition has a practical and effective effect of helping the body to discharge lead.

Description

Technical Field

[0001] The invention belongs to the production field of complementary food nutritional supplements, and relates to a formula and production process of a children's complementary food nutritional supplement that helps to excrete lead, and is suitable for children with abnormal blood lead levels. Background Art

[0002] Lead is a blue-grey metal and a non-degradable environmental pollutant. It can accumulate in the environment for a long time. It mainly enters the human body through food, soil, water and air. Some of it accumulates in the human body, causing damage to the human nervous, hematopoietic, immune, digestive, growth and other systems and organs to varying degrees. More seriously, lead damages the cognitive and neurobehavioral brain functions of infants and young children, affecting growth and intellectual development. Lead is very harmful to children, especially to the brain. It can cause children to have difficulty concentrating, fatigue, loss of appetite, memory loss, hyperactivity, insomnia, dizziness, dreaminess, slow reaction, irritability, and temper tantrums. Some children may also suffer from anemia, and even serious children may have convulsions, coma and other symptoms. How to eliminate the accumulated lead in the body has become a focus of attention.

[0003] Dietary fiber has strong selectivity and adsorption for lead substances, and can form complex precipitation with lead. Studies have shown that the liver has a good metabolic effect, and lead in the human body can be excreted through bile. Lead precipitated in human bones can be excreted through the blood system, liver, and gallbladder to the intestines, and then part of it will be reabsorbed. If lead meets dietary fiber in the intestines, the interaction between the two will produce insoluble substances, which will eventually be excreted from the body through excrement. Oligosaccharide raw materials in dietary fiber also help create a breeding environment for bifidobacteria in the intestines, reduce the production of harmful bacteria, reduce the burden on the liver due to detoxification, and improve the body's immunity to lead.

[0004] Minerals, vitamins and other nutrients also have the effect of excreting lead. Selenium can produce selenium-protein complexes with lead, a metal ion with a positive charge, to reduce the body's absorption of lead substances and avoid the accumulation of lead; zinc can increase the activity of enzymes in the human body, stimulate the excretion of lead, avoid the oxidation of sulfhydryl groups by hydroxyl radicals, promote the synthesis of glutathione, and reduce the damage of lead poisoning to the human body; the correlation between iron, calcium and lead exposure, supplementation of iron ions can improve the damage of the blood-brain barrier and neuronal apoptosis caused by lead exposure; supplementation of VC and folic acid can improve the stress response caused by lead poisoning; Group B can improve the symptoms of lead poisoning and promote the recovery of physiological functions; VD can promote calcium absorption, thereby assisting the body in excreting lead.

[0005] Medicinal and edible ingredients contain a large amount of amino acids, organic acids, polysaccharides, polyphenols and a large number of nutrients. After consumption, they can induce the body to synthesize metallothionein, which can then chelate with lead in the body while inhibiting the body's absorption of lead. It can also supplement the lack of metal chelators in the body, enhance the body's immunity, and play a role in excreting lead.

[0006] At present, the main lead removal drugs in medical treatment include complex-type lead removal agents such as calcium edetate (Ca-EDTA), calcium-promoting lead removal agent (Ca-DTPA) and sodium dithiosulfate succinate (NaDMS). Although their lead removal effect is strong, they need long-term intravenous injection and will also excrete other essential trace elements, causing the physiological activity of related enzymes to decrease or even inactivate. They are only suitable for emergency use. Children are more prone to lead accumulation than adults. In order to reduce the lead content in children's bodies, the prevention and treatment of lead poisoning needs to shift from clinical treatment to family health care prevention. How to develop a popular, economical food with lead removal function, mainly preventive, long-term use and no side effects is an urgent issue to be solved.

[0007] There are many chemical factors for excreting lead in natural foods, which can balance trace elements and excrete lead from the body without causing element deficiency. At the same time, the side effects are small, the form is easily accepted by children, and the intake is simple. The source is wide and the cost is low, which can reduce the probability of lead poisoning from the root. The present invention aims at the current high lead poisoning rate and lead poisoning hazards of children. In the supplementary food nutritional supplement, nutrients are added to dietary fiber, and medicine and food containing a variety of natural active ingredients are used. After consumption, the metal lead in the child's body is chelated and captured, thereby promoting the excretion of metal lead, reducing the accumulation of lead in the blood and tissues, and reducing the damage caused by lead poisoning. Summary of the invention

[0008] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a children's food supplement that helps to excrete lead and a preparation method thereof.

[0009] In order to achieve the above-mentioned object, the present invention adopts the following technical scheme: a children's complementary food nutritional supplement that helps to excrete lead, the addition amount per kilogram: 220-330 g of whole milk powder, 170-260 g of instant soy powder, 100-160 g of concentrated whey protein, 150-230 g of composite dietary fiber powder, 75-120 g of composite medicine and food powder, 35-55 g of DHA algae oil powder, 20-30 g of composite minerals, 3-5.5 g of composite vitamins, and 3-5 g of selenium-enriched yeast; Preferably, the compound dietary fiber powder is added in an amount of 150-230 g per kilogram: RS 3 Type resistant starch 80-130 g, stachyose 40-65 g, fucoidan 25-45 g, of which S 3Resistant starch and fucoidan can have strong selectivity and adsorption for lead substances, and can form complex precipitation with lead elements; stachyose can reduce the production of harmful bacteria in the intestines, thereby reducing the burden on the liver due to detoxification and improving the body's immunity to lead elements.

[0010] Preferably, the added amount of the composite medicine and food powder is 75-120 g per kilogram: 20-35 g chrysanthemum powder, 20-30 g wolfberry powder, 10-25 g roxburghii powder, 10-20 g sea buckthorn powder, and 8-15 g kudzu root powder, which is formulated with reference to ancient Chinese medicine prescriptions, wherein chrysanthemum and wolfberry are the main medicines for invigorating the spleen, detoxifying and removing lead, roxburghii is the minister medicine for relieving food stagnation, sea buckthorn is the adjuvant medicine for calming the liver and tranquilizing the mind, and kudzu root is the guiding medicine for removing blood stasis and detoxifying. All the medicines are combined to play the role of invigorating the spleen and stomach, removing dampness and removing lead.

[0011] Preferably, the amount of compound minerals added is 3-5.5 g per kilogram: 11-18 g of calcium carbonate, 2.5-4.2 g of sodium ferric ethylenediaminetetraacetate, and 0.12-0.25 g of zinc oxide. There is a great correlation between iron, calcium and lead exposure. Supplementing iron ions can improve the damage of the blood-brain barrier and neuronal apoptosis caused by lead exposure; zinc can increase the activity of enzymes in the human body, stimulate the excretion of lead, avoid the oxidation of sulfhydryl groups by hydroxyl radicals, promote the synthesis of glutathione, and reduce the damage of lead poisoning to the human body.

[0012] Preferably, the amount of compound vitamins added is 3-5.5 g per kilogram: 2-3.5 g of sodium L-ascorbate, 0.03-0.05 g of thiamine hydrochloride, 0.03-0.05 g of riboflavin, 0.01-0.022 g of retinyl acetate, 0.0035-0.0068 g of folic acid, 0.0003-0.0005 g of cholecalciferol, and 0.000038-0.000055 of cyanocobalamin. VC and folic acid can improve the stress response caused by lead poisoning; Group B can improve the symptoms of lead poisoning and promote the recovery of physiological functions; VD can promote calcium absorption, thereby assisting the body in excreting lead.

[0013] Preferably, the present invention contains high-content protein, DHA algae oil powder and selenium-enriched yeast while adding the above nutrients. Protein can improve the nutritional status of children with high blood lead disease, improve the body's immunity, and thus improve the body's lead metabolism ability; DHA algae oil powder has a good intervention effect on children's lead excretion. Studies have shown that docosahexaenoic acid (DHA) levels also show obvious abnormalities in children with abnormal blood lead levels. An appropriate amount of DHA supplementation can promote the body's lead excretion to a certain extent; selenium, as a negatively charged non-metallic ion, can combine with lead in the body to form a lead-selenium-protein complex, which is directly excreted from the body or excreted from the body through bile secretion, playing a detoxification and excretion role.

[0014] The principle of the material expansion pulsation circulation mixing technology used is that the material forms a suspended state under the action of the expansion pulsation airflow power and is involved in the expansion pulsation cycle. The kinetic energy of the high-speed rotating airflow and the fluid mechanics effect keep the material in a suspended state in the mixing chamber, and then rise, fall and circulate with the circulating airflow, so that different particles mix with each other and achieve a uniform mixing effect. It avoids the wear, dust and energy consumption problems caused by mechanical stirring in traditional mixing equipment, maintains the purity of the material during the mixing process, and improves the mixing efficiency and quality. At the same time, the use of mixed inert gas filling can effectively prevent the attenuation of nutrients in complementary food nutritional supplements.

[0015] The preparation method adopted is a material expansion pulsating circulation type mixing technology, a nitrogen and carbon dioxide composite inert gas filling process in a certain proportion, and the steps include the following: (1) Material expansion pulsating circulation mixing technology: first weigh 200 kg of whole milk powder, instant soybean powder, concentrated whey protein, compound dietary fiber powder, compound medicine and food powder, DHA algae oil powder, compound minerals, compound vitamins, and selenium-enriched yeast in proportion; The whole milk powder, concentrated whey protein powder, compound minerals and compound vitamins are sieved through an oscillating sieve and then sucked into a material expansion pulsation circulation tank by a vacuum feeder for standby use; The composite medicine and food homologous powder and selenium-enriched yeast are sucked into the material expansion pulsation circulation premixing tank through a vacuum feeder, mixed to obtain mixed powder I, and the mixed powder I is transported to the material expansion pulsation circulation tank by nitrogen positive pressure; The instant bean powder, compound dietary fiber powder and DHA algae oil powder are sieved through an oscillating screen and sucked into a material expansion pulsating circulation tank by a vacuum feeder, and finally mixed by a material expansion pulsating circulation type mixing technology to obtain a children's complementary food nutritional supplement that helps to excrete lead.

[0016] Mixing with inert gas: setting the ratio of inert gas mixed with nitrogen and carbon dioxide to 2-5:2 by controlling the human-machine interface of the canning machine control system, and after the total gas pressure reaches 0.4-0.6MPa, the gas is filled into the gas storage tank, and mixed for 5-10 minutes at a flow rate of 300-1000L / h and a temperature of 20-25°C. The obtained mixed powder of children's complementary food nutritional supplement that helps to excrete lead is mixed through the mixed gas and then canned by a fully automatic bagged food canning machine to obtain a children's complementary food nutritional supplement that helps to excrete lead.

[0017] Mixed inert gas filling canning: Under the mediation of mixed gas with a mixed gas flow rate of 500-1000L / h and a mixed gas pressure of 0.6-1.0MPa, quantitative canning is carried out through a fully automatic bagged food canning machine, the filling amount is controlled to be 18±0.18g / bag, and the quantitative packaging accuracy is more than 99.9%. The residual oxygen content of the obtained children's complementary food nutritional supplement that helps to excrete lead is <3%, and the quality rate is ≥99%. DETAILED DESCRIPTION

[0018] The present invention is described in further detail below in conjunction with actual practice, but the embodiments of the invention are not limited thereto. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art. The test methods for which specific experimental conditions are not specified in the following examples are usually in accordance with conventional experimental conditions. Unless otherwise specified, the reagents and raw materials used in the present invention can be obtained commercially.

[0019] Example 1: A method for preparing a children's complementary food nutritional supplement that helps to excrete lead: weigh 43.65 kg of whole milk powder, 40 kg of instant soy powder, 40 kg of concentrated whey protein, 34.6 kg of composite dietary fiber powder, 17.7 kg of composite medicine and food powder, 8 kg of DHA algae oil powder, 4.5 kg of composite minerals, 0.8 kg of composite vitamins, and 0.75 kg of selenium-enriched yeast. kg totaling 190kg; whole milk powder, concentrated whey protein powder, composite minerals and composite vitamins are sieved through an oscillating sieve and then sucked into a material expansion pulsating circulation tank through a vacuum feeder for standby use; composite medicine and food powder and selenium-enriched yeast are sucked into a material expansion pulsating circulation premixing tank through a vacuum feeder, mixed to obtain mixed powder I, and the mixed powder I is transported to the material expansion pulsating circulation tank through nitrogen positive pressure; instant soy powder, composite dietary fiber powder and DHA algae oil powder are sieved through an oscillating sieve and sucked into a material expansion pulsating circulation tank through a vacuum feeder, and finally mixed through a material expansion pulsating circulation mixing technology to obtain a children's complementary food nutritional supplement that helps to excrete lead.

[0020] The contents of a children's complementary food nutritional supplement that helps to excrete lead are placed in a silo of a fully automatic bagged food canning machine, and the ratio of nitrogen to carbon dioxide is set to 2:2 by controlling the human-machine interface of the control system of the fully automatic bagged food filling machine. After the total gas pressure reaches the set pressure of the two gases, the gas is filled into the gas storage tank, and a mixing process is performed under the conditions of a mixed gas pressure of 0.4MPa, a flow rate of 300L / h, a temperature of 20°C, and a mixing time of 5 minutes; then, quantitative filling and heat sealing treatment are performed by the fully automatic bagged food canning machine under the mediation of a mixed gas with a mixed gas flow rate of 500L / h and a mixed gas pressure of 0.6MPa, the filling amount is controlled to be 18±0.18 g / bag, and the quantitative filling accuracy is above 99.9%, so as to obtain a children's complementary food nutritional supplement that helps to excrete lead; the residual oxygen content of the product is <0.25%, and the rate of excellent products is ≥99%.

[0021] Example 2: A method for preparing a children's complementary food nutritional supplement that helps to excrete lead: weigh 49.5 kg of whole milk powder, 45 kg of instant soy powder, 42 kg of concentrated whey protein, and RS 3 15 kg of resistant starch, 7 kg of stachyose, 5 kg of fucoidan, 4.2 kg of chrysanthemum powder, 2.1 kg of wolfberry powder, 2.49 kg of roxburghii powder, 2.5 kg of sea buckthorn powder, 1.69 kg of kudzu root powder, 8.4 kg of DHA algae oil powder, 3 kg of calcium carbonate, 0.66 kg of sodium ferric EDTA, 0.035 kg of zinc oxide, 0.6 kg of sodium L-ascorbate, 0.008 kg of thiamine hydrochloride, 0.008 kg of riboflavin, 0.003 kg of retinyl acetate, 0.0011 kg of folic acid, 0.00008 kg of cholecalciferol, 0.00001 kg of cyanocobalamin, 0.89 kg of selenium-enriched yeast, a total of 190 kg; whole milk powder, concentrated whey protein powder, calcium carbonate, sodium ferric EDTA, zinc oxide, sodium L-ascorbate, thiamine hydrochloride, riboflavin, retinyl acetate, folic acid, cholecalciferol, and cyanocobalamin are sieved through an oscillating sieve and sucked into a material expansion pulsating circulation tank through a vacuum feeder for standby use; chrysanthemum powder, wolfberry powder, roxburghii powder, sea buckthorn powder, kudzu root powder, and selenium-enriched yeast are sucked into a material expansion pulsating circulation premixing tank through a vacuum feeder, mixed to obtain a mixed powder I, and the mixed powder I is transported to the material expansion pulsating circulation tank by nitrogen positive pressure; instant soybean powder, RS 3Resistant starch, stachyose, fucoidan, and DHA algae oil powder are sieved through an oscillating screen and sucked into a material expansion pulsating circulation tank through a vacuum feeder. They are finally mixed through a material expansion pulsating circulation mixing technology to obtain a children's complementary food nutritional supplement that helps to excrete lead. The contents of a children's complementary food nutritional supplement that helps to excrete lead are placed in a silo of a fully automatic bagged food canning machine, and the ratio of nitrogen to carbon dioxide is set to 3:2 by controlling the human-machine interface of the control system of the fully automatic bagged food filling machine. After the total gas pressure reaches the set pressure of the two gases, the gas is filled into the gas storage tank, and a mixing process is performed under the conditions of a mixed gas pressure of 0.5 MPa, a flow rate of 600 L / h, a temperature of 21°C, and a mixing time of 7 minutes; then, quantitative filling and heat sealing treatment are performed by the fully automatic bagged food canning machine under the mediation of a mixed gas with a mixed gas flow rate of 600 L / h and a mixed gas pressure of 0.8 MPa, the filling amount is controlled to be 18±0.18 g / bag, and the precision of quantitative filling is above 99.9%, so as to obtain a children's complementary food nutritional supplement that helps to excrete lead; the residual oxygen content of the product is <0.25%, and the rate of excellent products is ≥99%.

[0022] Example 3: Experimental evaluation of the effect of a children's food supplement that helps to excrete lead (prepared in Example 1) (1) Animal experiment to evaluate lead excretion effect 1) Establishment of lead poisoning rat model Fifty clean-grade SD (SpragIIe Dawley) male rats, weighing 160-180 g, were selected. The rats were kept in an SPF animal room with a room temperature of 20-22 ℃, a relative humidity of 50-60%, and natural light. During the experiment, the rats had free access to food and water (drinking water was sterilized by high-pressure steam, and feed was sterilized by ultraviolet irradiation). After being adaptively fed with ordinary feed for 7 days, the rats were randomly divided into 5 groups, namely, blank control group (group A), model control group (group B), low-dose intervention group of children's complementary food nutritional supplements that help excrete lead (group C), normal-dose intervention group of children's complementary food nutritional supplements that help excrete lead (group D), and high-dose intervention group of children's complementary food nutritional supplements that help excrete lead (group E), with 10 rats in each group.

[0023] Lead acetate was prepared into a solution with a concentration of 2 g / L, and the rats were exposed to lead acetate solution at a concentration of 10 mg / kg body weight (i.e., the exposure volume of lead acetate solution for rats was 5 ml / kg). The model control group (group B), the low-dose intervention group of children's complementary food nutritional supplements that help excrete lead (group C), the normal-dose intervention group of children's complementary food nutritional supplements that help excrete lead (group D), and the high-dose intervention group of children's complementary food nutritional supplements that help excrete lead (group E) were exposed to lead acetate solution by oral gavage at a concentration of 10 mg / kg body weight (i.e., the exposure volume of lead acetate solution for rats was 5 ml / kg). The blank control group was given deionized water by gavage at the same volume as the exposed groups, once a day for 4 consecutive weeks to establish a rat model of lead poisoning.

[0024] The children's food supplement that helps to excrete lead is prepared according to Example 1.

[0025] 2) Experimental procedures Each rat in groups A and B was gavaged twice a day with 3 ml of deionized water at about 40°C; each rat in group C was gavaged twice a day with 0.15 g of the children's complementary food nutritional supplement of the present invention, which was diluted to 3 ml with deionized water at about 40°C during gavage (equivalent to a child consuming half a pack of the children's complementary food nutritional supplement of the present invention per day); each rat in group D was gavaged twice a day with 0.3 g of the children's complementary food nutritional supplement of the present invention, which was diluted to 3 ml with deionized water at about 40°C during gavage (equivalent to a child consuming one pack of the children's complementary food nutritional supplement of the present invention per day); each rat in group E was gavaged twice a day with 0.6 g of the children's complementary food nutritional supplement of the present invention, which was diluted to 3 ml with deionized water at about 40°C during gavage (equivalent to a child consuming two packs of the children's complementary food nutritional supplement of the present invention per day), with an interval of about 6 hours between two gavages. During the experiment, the rats were fed with complete feed, and they could eat and drink water normally.

[0026] The intervention lasted for 28 days (4 weeks). The experimental animals were weighed every 7 days. Twenty-four hours after the last intervention, the rats were anesthetized, whole blood samples were collected, and kidney and liver samples were collected by dissection. After digestion, the lead content was determined using a graphite furnace atomic absorption spectrophotometer (or ICP-MS or other methods).

[0027] 3) Effects of children's complementary food nutritional supplements that help excrete lead on the body weight, physical signs and behavior of lead-poisoned rats The rats in each experimental group drank water freely during the experiment, and there was no obvious abnormality in feed intake. From the perspective of rat physical signs and behavior, the survival status and physical signs of rats in group A were good; the overall performance of rats in the other four groups was poor at the beginning of the experiment, with poor mental state, often showing aggressive, nervous, oversensitive behaviors, and signs of poisoning such as tongue swelling, reduced appetite, irregular and loose fur accompanied by hair loss, mental depression, hemiplegia, etc. However, as the experiment progressed, the conditions of rats in groups C, D, and E all improved to varying degrees, while the condition of rats in group B did not improve and was the most serious.

[0028] Depend on Figure 1 , Figure 2 It can be seen that the weight and growth of rats in group A have been higher than those in other lead-poisoned groups, and the weight and growth of rats in group B have been lower than those in other experimental groups. In addition, there was a significant difference in the average weekly weight gain of rats in groups C, D, and E, which were given children's complementary food nutritional supplements with different daily intakes that help excrete lead, compared with the model control group B (P < 0.05), but there was no significant difference in the average weekly weight gain of rats between groups E and A (P > 0.05).

[0029] It shows that administering lead acetate solution can significantly inhibit the growth and development of rats; the intake of children's complementary food nutritional supplements that help excrete lead can supplement the nutrients needed by the body of experimental animals, improve the body's immune ability of experimental animals, and have a certain promoting effect on the health recovery of lead-poisoned rats.

[0030] 4) Effects of children's complementary food nutritional supplements that help excrete lead on the lead content in the whole blood, liver and kidney of lead-poisoned rats According to the National Occupational Exposure Hazard Classification Standard (GBZ230-2010), lead is a highly hazardous substance. Lead can produce multiple toxic effects such as nerves, reproduction, cardiovascular, liver, kidney, and immunity. After lead poisoning, it is distributed to various tissues and organs through blood circulation. The liver and kidney play an important role in the accumulation, detoxification and excretion of lead, making them important target organs for lead toxicity. Therefore, this experiment uses whole blood lead, liver lead, and kidney lead as lead excretion research indicators.

[0031] The lead content in the whole blood, liver and kidney of the experimental rats was statistically analyzed in Table 1. The lead content in the whole blood, liver and kidney of the rats in group B was higher than that in group A (P < 0.05), indicating that the lead poisoning model was successfully established.

[0032] The lead levels in the whole blood, liver and kidney of rats in groups D and E, which were given different gradient daily intakes of children's complementary food nutritional supplements that help excrete lead, were lower than those in group B. Figure 3 The comparison chart of the blood lead, liver lead and kidney lead clearance rates of lead-poisoned rats with children's complementary food nutritional supplements that help to excrete lead shows that as the daily intake of children's complementary food nutritional supplements that help to excrete lead increases, the blood lead, liver lead and kidney lead clearance rates of lead-poisoned rats also increase. Among them, the blood lead, liver lead and kidney lead clearance rates of rats in group E after experimental intervention were the highest at 69.41%, 88.52% and 43.10% respectively.

[0033] This indicates that children's food supplements that help excrete lead have a promoting effect on the excretion of lead in the liver and kidneys, and the clearance rate increases with the increase of intake.

[0034] Group Group A Group B Group C Group D Group E Whole blood lead (ng / ml) <![CDATA[2.18±0.16 a ]]> <![CDATA[50.62±1.01 e ]]> <![CDATA[27.33±2.15 d ]]> <![CDATA[19.66±0.64 c ]]> <![CDATA[15.48±1.82 b ]]> Liver lead (ng / ml) <![CDATA[6.38±0.44 a ]]> <![CDATA[198.14±2.38 d ]]> <![CDATA[30.81±1.74 c ]]> <![CDATA[29.39±1.56 c ]]> <![CDATA[22.74±2.25 b ]]> Renal lead (ng / ml) <![CDATA[35.37±2.66 a ]]> <![CDATA[361.59±1.71 e ]]> <![CDATA[265.15±4.75 d ]]> <![CDATA[225.99±3.63 c ]]> <![CDATA[205.73±3.31 b ]]> Note: Different letters in the same indicator in the figure indicate significant differences between the values ​​(P < 0.05) (2) Test to evaluate the lead removal effect 1) Experimental design and personnel grouping requirements Subject criteria: Voluntary children with a history of lead exposure and high blood lead levels. Blood lead concentration ≥ 100 μg / L; no allergy to the ingredients of the present invention and no drug allergy.

[0035] Exclusion criteria for subjects: those with cognitive dysfunction, recent use of antipsychotic drugs; those with congenital malformations, coagulation dysfunction or malignant tumors; those with severe liver and kidney dysfunction, hematopoietic system abnormalities or systemic infections.

[0036] 98 children with lead poisoning were selected and divided into two groups by lottery. There were 49 cases in the control group, including 29 males and 20 females, aged 2-7 years old, with an average age of 4.88±0.89 years old, and a course of disease of 1-6 months, with an average age of 3.98±0.78 months; there were 49 cases in the observation group, including 30 males and 19 females, aged 2-7 years old, with an average age of 4.53±0.78 years old, and a course of disease of 1-6 months, with an average age of 3.52±0.54 months.

[0037] 2) Experimental methods Control group: Calcium sodium edetate combined with water-soluble vitamins. Each time, 25 mg / kg of calcium sodium edetate injection was mixed with 5% glucose sodium chloride injection to make a 0.2% solution, which was intravenously dripped once a day for 3 consecutive days and stopped for 4 days; 2 bottles of water-soluble vitamins were taken each time, mixed with 5% glucose sodium chloride injection, and intravenously dripped once a day for 28 consecutive days.

[0038] Observation group: The patients in the control group were treated with the children's food supplement for lead excretion according to the present invention, twice a day, 1 bag each time, and each bag was mixed with about 120 ml of warm water for 28 consecutive days.

[0039] The children's food supplement that helps to excrete lead is prepared according to Example 1.

[0040] Effect of children's complementary food nutritional supplements that help excrete lead on blood lead levels in children with lead poisoning Calcium sodium edetate (CaNa2-EDTA) is a commonly used lead-removing drug in clinical practice. It reacts with lead to form a stable water-soluble complex, which is then excreted in the urine.

[0041] Comparison of blood lead levels in the two groups of lead poisoned children before and after treatment Figure 4 It can be seen that there was no significant difference in the blood lead levels of the two groups of children before treatment. After treatment, the blood lead levels of both groups were higher than before treatment (P < 0.05). And after 28 days of treatment, the blood lead level of the observation group was lower than that of the control group (P < 0.05). There were no adverse reactions in the two groups of children during the experiment.

[0042] The results show that the consumption of children's complementary food nutritional supplements that help to excrete lead has a significant effect on assisting the treatment of children with lead poisoning. After consumption, the absorption of lead by the body of children with lead poisoning can be inhibited, and the steady excretion of lead in the body can be promoted. Moreover, after consumption, the adverse reactions of children to the lead excretion treatment will not increase. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is a diagram showing the effect of children's complementary food nutritional supplements that help excrete lead on the weight changes of lead-poisoned rats (groups: Group A: blank control group; Group B: model control group; Group C: low-dose intervention group of children's complementary food nutritional supplements that help excrete lead; Group D: normal-dose intervention group of children's complementary food nutritional supplements that help excrete lead; Group E: high-dose intervention group of children's complementary food nutritional supplements that help excrete lead); Figure 2 This is a comparative diagram of the effects of children's complementary food nutritional supplements that help excrete lead on the average weekly weight growth of lead-poisoned rats (different letters in the same indicator in the figure indicate significant differences between the values ​​(P < 0.05), groups: Group A: blank control group; Group B: model control group; Group C: low-dose intervention group of children's complementary food nutritional supplements that help excrete lead; Group D: normal-dose intervention group of children's complementary food nutritional supplements that help excrete lead; Group E: high-dose intervention group of children's complementary food nutritional supplements that help excrete lead); Figure 3 This is a comparative diagram of the effects of children's complementary food nutritional supplements that help excrete lead on the clearance rates of blood lead, liver lead and kidney lead in lead-poisoned rats (different letters in the same index in the figure indicate significant differences between the values ​​(P < 0.05), groups: Group A: blank control group; Group B: model control group; Group C: low-dose intervention group of children's complementary food nutritional supplements that help excrete lead; Group D: normal-dose intervention group of children's complementary food nutritional supplements that help excrete lead; Group E: high-dose intervention group of children's complementary food nutritional supplements that help excrete lead); Figure 4 This is a graph showing the effect of children's complementary food nutritional supplements that help excrete lead on the blood lead content of children with lead poisoning (different letters in the figure indicate significant differences between the values, P < 0.05).

Claims

1. A children's food supplement that helps to excrete lead. It is characterized in that The complementary food nutritional supplement is prepared by using whole milk powder and instant bean powder as main raw materials, adding seven vitamins, four minerals, five medicinal and edible substances, five dietary fibers and other ingredients, and is prepared by a material expansion pulsating circulation type mixing technology and a composite inert gas (nitrogen, carbon dioxide) filling process according to a certain proportion; The ingredients include whole milk powder, instant soy powder, concentrated whey protein, compound dietary fiber powder (RS 3 The invention comprises the following components: a type of resistant starch, stachyose, fucoidan), a compound edible and medicinal powder (chrysanthemum powder, wolfberry powder, roxburghii powder, sea buckthorn powder, kudzu root powder), DHA algae oil powder, a compound mineral substance (calcium carbonate, sodium ferric EDTA, zinc oxide), a compound vitamin (sodium L-ascorbate, thiamine hydrochloride, riboflavin, retinyl acetate, folic acid, cholecalciferol, cyanocobalamin), and selenium-enriched yeast. Through the intervention experiment of high-lead rat model and the observation and comparison of the trial effect of voluntary test children with a history of lead exposure and high blood lead content, it is proved that the invention has a practical and effective effect in helping the body to excrete lead.

2. Preparation of a children's food supplement that helps to remove lead, It is characterized in that The following steps are involved: Weigh whole milk powder, instant soy powder, concentrated whey protein, compound dietary fiber powder, compound medicine and food powder, DHA algae oil powder, compound minerals, compound vitamins, and selenium-enriched yeast according to the proportion; The whole milk powder, concentrated whey protein powder, compound minerals and compound vitamins are sieved through an oscillating sieve and then sucked into a material expansion pulsation circulation tank by a vacuum feeder for standby use; The composite medicine and food homologous powder and selenium-enriched yeast are sucked into the material expansion pulsation circulation premixing tank through a vacuum feeder, mixed to obtain mixed powder I, and the mixed powder I is transported to the material expansion pulsation circulation tank by nitrogen positive pressure; The instant bean powder, the composite dietary fiber powder and the DHA algae oil powder are sieved through an oscillating sieve and sucked into a material expansion pulsating circulation tank by a vacuum feeder, and finally mixed by a material expansion pulsating circulation type mixing technology to obtain a children's complementary food nutritional supplement that helps to excrete lead; The ratio of each component of the inert gas is set by controlling the human-machine interface of the canning machine control system. After the total gas pressure reaches the set pressure of the two gases, the gas is filled into the gas storage tank, and the obtained mixed powder of the children's complementary food nutritional supplement that helps to remove lead is mixed through the mixed gas and then canned by the fully automatic bagged food canning machine to obtain the children's complementary food nutritional supplement that helps to remove lead; (1) The ratio of the mixed inert gas is 2-5:2 of nitrogen to carbon dioxide; (2) The mixing under the mixed gas is carried out under the mediation of nitrogen and carbon dioxide mixed gas during the mixing process, the pressure of the mixed gas is 0.4-0.6 MPa, the flow rate is 300-1000 L / h, the temperature is 20-25 ° C, and the mixing time is 5-10 minutes, so as to obtain a children's complementary food nutritional supplement that helps to excrete lead; (3) The preparation of the children's food supplement that helps to excrete lead under the mediated mixed gas is a powder of the children's food supplement that helps to excrete lead, which is prepared by a quantitative canning and heat sealing process under the mediated mixed gas of nitrogen and carbon dioxide; (4) The quantitative canning and heat sealing treatment is carried out by an automatic bagged food canning machine under the mediation of a mixed gas with a mixed gas flow rate of 500 to 1000 L / h and a mixed gas pressure of 0.6 to 1.0 MPa, with the filling volume controlled to be 18±0.18 g / bag and the quantitative packaging accuracy being above 99.9%. The residual oxygen content of the obtained children's complementary food nutritional supplement that helps to excrete lead is <3%, and the rate of excellent products is ≥99%.

3. A children's food supplement that helps to remove lead. It is characterized in that Added amount per kilogram: whole milk powder 220-330 g, instant soy powder 170-260 g, concentrated whey protein 100-160 g, compound dietary fiber powder 150-230 g, compound medicine and food powder 75-120 g, DHA algae oil powder 35-55 g, compound minerals 20-30 g, compound vitamins 3-5.5 g, selenium-enriched yeast 3-5 g.

4. The children's food supplement that helps to remove lead according to claim 3, It is characterized in that The composite dietary fiber powder is prepared by compounding three dietary fiber powders according to the mass ratio, and the addition amount per kilogram is: RS 3 Type resistant starch 80-130 g, stachyose 40-65 g, fucoidan 25-45 g.

5. The children's food supplement that helps to remove lead according to claim 3, It is characterized in that The composite edible and medicinal powder is prepared by compounding five edible and medicinal powders according to a mass ratio, and the added amount per kilogram is: 20-35 g chrysanthemum powder, 20-30 g wolfberry powder, 10-25 g roxburghii powder, 10-20 g sea buckthorn powder, and 8-15 g kudzu root powder.

6. The children's food supplement that helps to excrete lead according to claim 3, It is characterized in that The composite mineral is prepared by compounding three mineral nutritional enhancers according to a mass ratio, and the added amount per kilogram is: 11-18g of calcium carbonate, 2.5-4.2g of sodium ferric ethylenediaminetetraacetate, and 0.12-0.25g of zinc oxide.

7. The children's food supplement that helps to expel lead according to claim 3, It is characterized in that The multivitamin is prepared by compounding seven vitamin nutritional enhancers according to a mass ratio, and the added amount per kilogram is: 2-3.5 g of L-sodium ascorbate, 0.03-0.05 g of thiamine hydrochloride, 0.03-0.05 g of riboflavin, 0.01-0.022 g of retinyl acetate, 0.0035-0.0068 g of folic acid, 0.0003-0.0005 g of cholecalciferol, and 0.000038-0.000055 of cyanocobalamin.

8. A gas conditioning process for the children's food supplement that helps to remove lead as claimed in claim 2, It is characterized in that The ratio of the mixed inert gas is 2-5:2 between nitrogen and carbon dioxide.

9. A gas conditioning process for the children's food supplement that helps to remove lead as claimed in claim 2, It is characterized in that The mixing method mediated by mixed gas is carried out under the conditions of mixed gas pressure of 0.4-0.6MPa, flow rate of 300-1000L / h, temperature of 20-25°C, and mixing time of 5-10 minutes.

10. A gas conditioning process for the children's food supplement that helps to remove lead as claimed in claim 2, It is characterized in that The nitrogen-mediated quantitative bagging and heat-sealing treatment method is carried out under the mediation of a mixed gas flow rate of 500-1000 L / h and a mixed gas pressure of 0.6-1.0 MPa, with a residual oxygen content of <3% and a high-quality rate of ≥99%.

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