Composition for rejuvenating, moistening and soothing skin and preparation method thereof

Abstract

The invention discloses a skin-activating, moistening and soothing composition and a preparation method thereof, and belongs to the technical field of cosmetics. The skin-activating, moistening, soothing and nourishing composition comprises a plant extract, a humectant, a chelating agent, a thickening agent, heparin sodium, an emollient, an antioxidant, an emulsifier, a pH regulator and a preservative. The plant extract is introduced, and the proportion of the plant extract, the humectant, the chelating agent, the thickening agent, the heparin sodium, the emollient, the antioxidant, the emulsifier and other components is reasonably adjusted, so that the composition is mild, non-irritating, easy to smear and absorb, capable of improving skin elasticity and excellent in soothing, compression-resisting, repairing and anti-aging effects; the preparation method provided by the invention is simple and easy to implement and convenient to operate, and the prepared composition is stable in quality.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to a skin activating, moisturizing and soothing composition and a preparation method thereof. Background Art

[0002] As people pay more attention to health and quality of life, consumer demand for cosmetics will continue to grow. Existing cosmetics on the market are of various types and have different functions, but there are still some common defects and limitations. For example, most cream cosmetics are too greasy, and the skin feels sticky after use. They are not easily absorbed, and cannot play a good anti-aging effect. They are also easy to irritate the skin and cause allergic reactions, such as redness, swelling, itching, etc. Summary of the invention

[0003] The object of the present invention is to overcome the deficiencies of the above-mentioned prior art and to provide a skin-revitalizing, nourishing and soothing composition and a preparation method thereof.

[0004] To achieve the above object, the technical solution adopted by the present invention is: In a first aspect, the present invention provides a skin revitalizing, nourishing and soothing composition, comprising the following components in percentage by mass: 0.5-5% plant extract, 5-6.9% humectant, 0.01-0.05% chelating agent, 0.05-0.25% thickener, 0.01-0.1% heparin sodium, 16-28% emollient, 0.1-0.5% antioxidant, 4.5-7% emulsifier, 1.1-1.3% pH adjuster, 0.1-0.5% preservative, and water as the remainder, wherein the plant extract comprises at least one of agave stem extract, kava root extract, Centella asiatica extract, gentian root extract, and white osmanthus extract.

[0005] As a preferred embodiment of the present invention, the plant extract includes agave stem extract, kava root extract, Centella asiatica extract, gentian root extract, and white osmanthus extract. The mass ratio of the kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract is (1-4):(0.5-2):(3-6):(0.5-2):10.

[0006] Agave stem extract is rich in active substances such as polysaccharides, amino acids, polyphenols, sterols, flavonoids, sulfur-containing amino acids, etc. It not only has a moisturizing effect, can promote skin metabolism, repair damaged skin tissue, but also has certain anti-inflammatory, antibacterial and antioxidant effects; Kava pepper root extract contains ingredients such as kavalactone and kavapyrone, which have anti-inflammatory and soothing effects on the skin; Centella asiatica extract contains active ingredients such as asiaticoside, hydroxymadecassoside, asiatic acid and hydroxymadecassoside, which can promote collagen synthesis and promote the secretion of mucopolysaccharides, and also have certain antibacterial, anti-inflammatory and antioxidant effects; Gentiana root extract contains active ingredients such as gentiopicroside, gentiobiose and gentisic acid, which can promote the secretion of cholesterol by epidermal cells, improve skin roughness, and have certain anti-inflammatory effects; White lily extract is rich in polyphenols and flavonoids, which has an antioxidant effect, can remove free radicals in the body, help delay skin aging, repair skin damage, and strengthen the skin barrier.

[0007] The inventors have found through research that the agave stem extract, kava root extract, centella asiatica extract, gentian root extract and white lily extract are compounded in a specific ratio, and there is an obvious synergistic effect, which can significantly improve the firmness and elasticity of the skin, and achieve the effects of soothing, anti-stress, repair and anti-aging. This may be because the extracts in the present invention have different effective ingredients, which can jointly promote the expression of anti-aging genes through different mechanisms and stimulate fibroblasts to produce collagen. In addition, the inventors unexpectedly found that the agave stem extract, kava root extract, centella asiatica extract, gentian root extract and white lily extract are compounded in a specific ratio to reduce the irritation of the composition with a high emulsifier content.

[0008] Furthermore, the mass ratio of the Piper methysticum root extract, Centella asiatica extract, Gentiana scabra root extract, Rhizoma Cibotii extract and Agave equilana stem extract is (2-3):(1-1.5):(4-5):(1-1.5):10.

[0009] Furthermore, the agave stem extract is prepared by the following method: the agave stem is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:15mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the agave stem extract.

[0010] Furthermore, the kava root extract is prepared by the following method: the kava root is crushed, dried, sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:15mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration, the filtrate is combined, concentrated, dried and sterilized to obtain the kava root extract.

[0011] Furthermore, the Centella asiatica extract is prepared by the following method: the whole Centella asiatica is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:10mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the Centella asiatica extract.

[0012] Furthermore, the gentian root extract is prepared by the following method: the gentian root is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:15mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration, the filtrate is combined, concentrated, dried, and then sterilized to obtain the gentian root extract.

[0013] Furthermore, the Pleurotus eryngii extract is prepared by the following method: the Pleurotus eryngii root is crushed, dried, sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the residue and filtrate are separated by filtration; the residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:10mL for ultrasonic extraction, and the residue and filtrate are separated by filtration, the filtrate is combined, concentrated, dried and sterilized to obtain the Pleurotus eryngii extract.

[0014] As a preferred embodiment of the present invention, the skin revitalizing, nourishing and soothing composition comprises the following components in percentage by mass: 2-4% plant extract, 6-6.5% humectant, 0.02-0.04% chelating agent, 0.16-0.2% thickener, 0.02-0.05% heparin sodium, 20.4-21.8% emollient, 0.12-0.3% antioxidant, 5.1-6.3% emulsifier, 1.1-1.3% pH adjuster, 0.1-0.5% preservative, and the remainder water.

[0015] As a preferred embodiment of the present invention, the moisturizing agent includes at least one of sodium hyaluronate, betaine, glycerol, glyceryl polyether-26, allantoin, panthenol, trehalose, dipropylene glycol, and butylene glycol.

[0016] As a preferred embodiment of the present invention, the thickener comprises carbomer.

[0017] As a preferred embodiment of the present invention, the emollient includes at least one of sea buckthorn oil, jojoba oil, cyclopentasiloxane, cyclohexasiloxane, caprylic / capric triglyceride, avocado butter, isopropyl myristate, ethylhexyl palmitate, hydrogenated polyisobutene, macadamia seed oil, hydrogenated polydecene, isodecyl neopentanoate, and dicaprylyl carbonate.

[0018] Further preferably, in the skin revitalizing, nourishing and soothing composition, the mass percentages of the components of the emollient are as follows: sea buckthorn oil 0.05-0.3%, jojoba oil 0.5-3%, cyclopentasiloxane 0.4-2.4%, cyclohexasiloxane 0.1-0.6%, caprylic / capric triglyceride 0.5-2%, avocado butter 0.1-0.5%, isopropyl myristate 3-10%, ethylhexyl palmitate 3-10%, hydrogenated polyisobutene 1-4%, and macadamia seed oil 0.05-0.3%.

[0019] As a preferred embodiment of the present invention, the emulsifier includes at least one of cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 stearate.

[0020] Further preferably, in the skin revitalizing, moisturizing and soothing composition, the mass percentages of the various components of the emulsifier are as follows: 1-3% cetearyl alcohol, 1-4% first mixed emulsifier, and 0.5-2% second mixed emulsifier, wherein the first mixed emulsifier is compounded with cetearyl alcohol-80 and cetearyl glucoside-20 in a mass ratio of (75-85):(15-25), and the second mixed emulsifier is compounded with glyceryl stearate-65 and PEG-100 stearate-35 in a mass ratio of (50-75):(25-50).

[0021] Furthermore, in the skin revitalizing, moisturizing and soothing composition, the mass percentages of the various components of the emollient are as follows: sea buckthorn oil 0.05-0.3%, jojoba oil 0.5-3%, cyclopentasiloxane 0.4-2.4%, cyclohexasiloxane 0.1-0.6%, caprylic / capric triglyceride 0.5-2%, avocado butter 0.1-0.5%, isopropyl myristate 3-10%, ethylhexyl palmitate 3-10%, hydrogenated polyisobutene 1-4%, and macadamia seed oil 0.05-0.3%; in the skin revitalizing, moisturizing and soothing composition, the mass percentages of the various components of the emulsifier are as follows: cetearyl alcohol 1.6-2.5%, a first mixed emulsifier 2-3%, and a second mixed emulsifier 0.8-1.5%. The inventors have found through research that when the components of the emulsifier and the moisturizer are within the above range, the water phase component and the oil component in the composition can be fully mixed to form a stable milky white paste, thereby making the texture of the composition smoother and more delicate, improving the spreadability and absorbability of the composition, and being able to gently nourish the skin.

[0022] As a preferred embodiment of the present invention, the pH regulator is a compound of arginine and water, wherein the mass percentage of arginine in the composition is 0.1-0.3%.

[0023] For example, the mass percentage of arginine in the composition can be 0.1%, 0.15%, 0.16%, 0.2%, 0.25%, 0.3% or a range consisting of any two groups of values ​​therein.

[0024] As a preferred embodiment of the present invention, the antioxidant comprises tocopherol acetate.

[0025] As a preferred embodiment of the present invention, the skin revitalizing, nourishing and soothing composition further comprises the following components in percentage by mass: 0.01-0.5% of essence.

[0026] The essence can be at least one of lavender oil, rose flower oil, rose flower oil, camellia oil, and daily essence.

[0027] In a second aspect, the present invention provides a method for preparing the skin-revitalizing, nourishing and soothing composition as described in the first aspect, comprising the following steps: S1, mixing water, moisturizing agent, chelating agent, thickener and heparin sodium, heating to 82-85°C, stirring and dissolving completely to obtain P1 phase; S2, mixing the emulsifier, emollient and antioxidant and heating them to 78-82°C to completely dissolve them to obtain phase P2; S3. Add phase P2 to phase P1 under stirring, homogenize for 5-8 minutes until completely dissolved, keep warm for 15-20 minutes, and defoam. S4, cooling the mixture after defoaming in step S3 to 64-66°C, adding pH regulators in sequence, stirring evenly, and then cooling to a temperature not higher than 45°C, adding plant extracts and preservatives in sequence, and stirring evenly; S5. Adjust the temperature of the mixture obtained in step S4 to 38-40° C., filter through a 100-mesh filter, and then discharge the mixture and allow it to stand to obtain the skin-revitalizing, nourishing and soothing composition.

[0028] Compared with the prior art, the present invention has the following beneficial effects: The present invention introduces plant extracts and reasonably mixes the proportions of plant extracts, moisturizers, chelating agents, thickeners, heparin sodium, emollients, antioxidants, emulsifiers and other components to make the composition mild and non-irritating, easy to apply and absorb, able to improve skin elasticity, and having excellent soothing, anti-stress, repairing and anti-aging effects; the preparation method provided by the present invention is simple and easy to operate, and the prepared composition has stable quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 A photograph of the composition obtained in Example 1 provided by the present invention; Figure 2 The test results provided for the effect test 1 of the present invention; Figure 3 The test results provided for the effect test example 2 of the present invention. DETAILED DESCRIPTION

[0030] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments and comparative examples, the purpose of which is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0031] Unless otherwise specified, the components and raw materials used in the embodiments and comparative examples of the present invention are all commercially available raw materials, and the components and raw materials used in each parallel experiment are all of the same kind.

[0032] The agave stem extracts in the following examples and comparative examples were prepared by the following method: The agave stem is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and filtered to separate the filter residue and the filtrate; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:15 mL, ultrasonically extracted at a power of 300 W for 45 minutes, filtered to separate the filter residue and the filtrate, the filtrate is combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, and sterilized at 100-120° C. after drying to obtain the agave stem extract.

[0033] The kava root extract in the following examples and comparative examples was prepared by the following method: The kava root is crushed, dried, sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL, ultrasonically extracted at a power of 300 W for 45 minutes, and filtered to separate the residue and the filtrate; the residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:15 mL, ultrasonically extracted at a power of 300 W for 45 minutes, filtered to separate the residue and the filtrate, the filtrate is combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, dried, and sterilized at 100-120° C. to obtain the kava root extract.

[0034] The Centella asiatica extract in the following examples and comparative examples was prepared by the following method: The whole herb of Centella asiatica is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration; the filter residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:10mL for ultrasonic extraction, and the filter residue and the filtrate are separated by filtration, the power of each ultrasonic extraction is 300W, and the time of each ultrasonic extraction is 45min. The filtrates are combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, and sterilized at 100-120°C after drying to obtain the Centella asiatica extract.

[0035] The gentian root extracts in the following examples and comparative examples were prepared by the following method: The gentian root is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1 g:20 mL, ultrasonically extracted at a power of 300 W for 45 min, and the residue and the filtrate are separated by filtration; the residue is added to an 80% ethanol solution at a solid-liquid ratio of 1 g:15 mL, ultrasonically extracted at a power of 300 W for 45 min, and the residue and the filtrate are separated by filtration, the filtrate is combined, and concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, and sterilized at 100-120° C. after drying to obtain the gentian root extract.

[0036] The Bletilla striata extract in the following examples and comparative examples was prepared by the following method: The root of Pleurotus eryngii is crushed, dried, and sieved, and then added to an 80% ethanol solution at a solid-liquid ratio of 1g:20mL for ultrasonic extraction, and the residue and the filtrate are separated by filtration; the residue is added to an 80% ethanol solution at a solid-liquid ratio of 1g:10mL for ultrasonic extraction, and the residue and the filtrate are separated by filtration, the power of each ultrasonic extraction is 300W, and the time of each ultrasonic extraction is 45min. The filtrates are combined, concentrated by rotary evaporation to 1 / 10 of the volume of the combined filtrate, and sterilized at 100-120°C after drying to obtain the Pleurotus eryngii extract.

[0037] In the present invention, the 80% ethanol solution refers to an ethanol aqueous solution in which the mass percentage of ethanol is 80%.

[0038] Plant extract S1 is a mixture of kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract. The mass ratio of the kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract is 3:1:4:1.5:10.

[0039] Plant extract S2 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 2:1.5:5:1:10.

[0040] Plant extract S3 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 1:0.5:6:2:10.

[0041] Plant extract S4 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 4:2:3:0.5:10.

[0042] The plant extract D1 is a mixture of Centella asiatica extract, gentian root extract, white osmanthus extract and agave stem extract, and the mass ratio of the Centella asiatica extract, gentian root extract, white osmanthus extract and agave stem extract is Centella asiatica extract: gentian root extract: white osmanthus extract: agave stem extract = 1:4:1.5:10.

[0043] The plant extract D2 is prepared by mixing gentian root extract, white osmanthus extract and agave stem extract in a mass ratio of gentian root extract: white osmanthus extract: agave stem extract = 4:1.5:10.

[0044] Plant Extract D3 In this comparative example, the plant extract consists of a Bletilla striata extract and an Agave equilana stem extract in a mass ratio of Bletilla striata extract: Agave equilana stem extract = 1.5:10.

[0045] The plant extract D4 is a mixture of gentian root extract and agave stem extract in a mass ratio of gentian root extract: agave stem extract = 4:10.

[0046] Plant extract D5 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 0.1:0.1:0.1:0.1:10.

[0047] Plant extract D6 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 0.5:0.5:0.5:0.5:10.

[0048] Plant extract D7 is prepared by mixing kava root extract, Centella asiatica extract, gentian root extract, iris extract and agave stem extract in a mass ratio of kava root extract: Centella asiatica extract: gentian root extract: iris extract: agave stem extract = 0.5:0.5:1:0.5:10.

[0049] Embodiment 1~4 The embodiment of the skin revitalizing, nourishing and soothing composition of the present invention, the formula of the skin revitalizing, nourishing and soothing composition is shown in Table 1 below.

[0050] The preparation method of the skin-revitalizing, nourishing and soothing composition is as follows: S1. Add the components of phase P1 into the main machine, heat to 82-85°C, stir and dissolve completely to obtain phase P1; S2, add the components of P2 phase into the oil phase pot, heat to 78-82°C until completely dissolved, and obtain P2 phase; S3. Evacuate the main machine, and pump the P2 phase into the P1 phase under stirring conditions, homogenize for 5-8 minutes, stir and dissolve completely, keep warm for 15-20 minutes, and defoam; S4, cooling the mixture after defoaming in step S3 to 64-66°C, adding the components of phase P3 in sequence, stirring evenly, and then cooling to a temperature not higher than 45°C, adding phase P4, phase P5 and phase P6 in sequence, and stirring evenly; S5. The mixture obtained in step S4 is adjusted to a temperature of 38-40° C., filtered through a 100-mesh filter, and then discharged and allowed to stand to obtain the composition.

[0051] Table 1 Example 5 The difference between this embodiment and embodiment 1 is that the plant extract used in this embodiment is plant extract S2.

[0052] Example 6 The difference between this embodiment and embodiment 1 is that the plant extract used in this embodiment is plant extract S3.

[0053] Example 7 The difference between this embodiment and embodiment 1 is that the plant extract used in this embodiment is plant extract S4.

[0054] Comparative Example 1 The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D1.

[0055] Comparative Example 2 The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D2. Comparative Example 3 The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D3.

[0056] Comparative Example 4 The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D4.

[0057] Comparative Example 5 The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D5.

[0058] Comparative Example 6 The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D6.

[0059] Comparative Example 7 The difference between this comparative example and Example 1 is that the plant extract used in this comparative example is plant extract D7.

[0060] Comparative Example 8 The difference between this comparative example and Example 1 is that in this comparative example, the mass percentage of cetearyl alcohol in the composition is 6%, the mass percentage of caprylic / capric triglyceride in the composition is 5%, and the mass percentage of water in the P1 phase in the composition is 53.665%.

[0061] The compositions obtained in the above examples and comparative examples were respectively placed in transparent containers. It was found through observation that each composition was a stable milky white paste. For example, the appearance of the composition provided in Example 1 was as follows: Figure 1 shown.

[0062] Effect test example 1 With plant extract S1 (corresponding to S1 group), plant extract S2 (corresponding to S2 group), plant extract S3 (corresponding to S3 group), plant extract S4 (corresponding to S3 group), plant extract D1 (corresponding to D1 group), plant extract D2 (corresponding to D2 group), plant extract D3 (corresponding to D3 group), plant extract D4 (corresponding to D4 group), plant extract D5 (corresponding to D5 group), plant extract D6 (corresponding to D6 group), and plant extract D7 (corresponding to D7 group) as samples, the following tests were carried out: Human dermal fibroblasts (HDF) were subcultured and when the cell density reached 80-90%, the cells were cultured at 12,000 / cm 2 The cells were seeded at a density of 100 μL in a 6-well plate in a culture medium containing 10% FBS and 1% double antibody. After overnight attachment, the original culture medium was discarded, and an equal amount of PBS buffer solution (as a control) and samples of each group were added to the corresponding wells, and culture was continued for 48 h. After 48 h of culture, the original culture medium was discarded, and the cells were washed with phosphate buffered saline (DPBS). After the cells were digested with trypsin, they were washed with phosphate buffered saline (DPBS), and 500 μL of lysis buffer for RNA extraction was added to each well. The cells were resuspended by pipetting until no cell precipitation was visible and the suspension was basically clear (this indicated that the cells had been fully lysed), and then the cell suspension was temporarily stored at -80°C. According to the operating procedures of the commercially available RNA extraction kit, RNA was extracted from each group, and the extracted RNA was dissolved in enzyme-free water, and its concentration was determined by a micro-spectrophotometer. Reverse transcription was performed according to the kit method, and 1000 ng of RNA was used for reverse transcription and qPCR. qPCR was used to detect the expression level of the COL1A1 gene related to collagen synthesis.

[0063] Test results such as Figure 2 shown.

[0064] from Figure 2 It can be seen that compared with the control group, the plant extracts used in groups S1 to S4, including kava root extract, centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract, significantly increased the expression level of the COL1A1 gene related to collagen synthesis. This shows that the plant extracts used in the present invention can promote the synthesis of collagen and have anti-aging effects.

[0065] From group D1 to group D4, it can be seen that group D3 to group D4 only used two extracts, and the expression level of COL1A1 gene was low; compared with group D3 to group D4, group D2 contained three extracts and group D1 contained four extracts, and the expression level of COL1A1 gene in group D1 and group D2 was significantly improved. This shows that there is a synergistic effect between kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract. Only when the above five extracts coexist, the synergistic effect between the components can be significantly enhanced.

[0066] It can be seen from groups S1 to S4 and groups D5 to D7 that when Kava root extract, Centella asiatica extract, Gentiana root extract, Viburnum tiliaceum extract and Agave stem extract are compounded in appropriate proportions, the up-regulation effect on the expression level of COL1A1 is more obvious.

[0067] Effect test example 2 Hyaluronic acid is synthesized by fibroblasts in the dermis. More hyaluronic acid in the skin can reduce the denaturation rate of collagen and elastin, slow down the denaturation rate from soluble protein to insoluble protein, improve the colloidal solution environment between skin cells, and thus make the original wrinkles formed by the space between cells smooth. Ultraviolet (UV) irradiation can lead to the production of hyaluronidase in the skin, degrade hyaluronic acid, and cause photoaging of the skin. Hyaluronidase is a type of glycosidase widely distributed in nature, which degrades hyaluronic acid (HA) by acting on β-1,3 or β-1,4 glycosidic bonds.

[0068] The present invention uses the compositions obtained in the above embodiments and comparative examples as samples, and uses the ELISA method to detect the inhibitory effect of each sample on hyaluronidase; prepares a hyaluronidase ELISA kit, and performs the following tests according to the instructions of the kit: (1) The sample was mixed with PBS buffer solution (pH 7) at a ratio of 1:29 to prepare the test sample. After equilibration at room temperature for 20 minutes, the required strips were removed from the aluminum foil bag. (2) Set up blank wells, control wells, and sample wells. Add 50 μL of hyaluronidase (concentration of 1000 U / L) to the control wells and sample wells respectively. Do not add enzyme to the blank wells. Incubate at 37°C for 10 min. Then add the test sample to the sample wells. Do not add enzyme to the blank wells and control wells. Incubate for 10 min. Wash the plate 5 times. (3) Except for the blank wells, add 100 μL of horseradish peroxidase-labeled detection antibody to the control wells and sample wells, seal each well with a sealing film, and incubate at 37°C in a constant temperature incubator for 60 min; (4) Discard the liquid, pat dry with absorbent paper, and wash the plate three times; (5) Add 50 μL of substrate A and B to each well and incubate at 37°C in the dark for 15 min; (6) Add 50 μL of stop solution to each well. Within 15 min, measure the OD value of each well at a wavelength of 450 nm and calculate the inhibition rate according to the following formula: Inhibition rate (%) = (OD 空白孔 +OD 对照孔 -OD 样品孔 ) / (OD 空白孔 +OD 对照孔 )×100%.

[0069] Test results such as Figure 3 shown.

[0070] from Figure 3 It can be seen that the plant extracts used in the embodiments of the present invention contain kava root extract, Centella asiatica extract, gentian root extract, white osmanthus extract, and agave stem extract, which have a certain inhibitory effect on hyaluronidase and have a good soothing effect.

[0071] Compared with Example 1, the plant extracts used in Comparative Examples 3 to 4 contain only two extracts, and the inhibition rate of hyaluronidase is significantly reduced; relative to Comparative Examples 3 to 4, the plant extract in Comparative Example 1 contains four extracts, and the plant extract in Comparative Example 2 contains three extracts, and the inhibition rate of hyaluronidase in Comparative Examples 1 to 2 is significantly higher than that in Comparative Examples 3 to 4. This shows that there is a synergistic effect between the Kava Piper melongena root extract, Centella asiatica extract, Gentiana root extract, White Vitex extract, and Agave stem extract, and only when the above five extracts exist together can the inhibitory effect of the composition on hyaluronidase be significantly enhanced.

[0072] It can be seen from Example 1, Examples 5 to 7 and Comparative Examples 5 to 7 that when the Piper methysticum root extract, Centella asiatica extract, Gentiana root extract, Viburnum tiliaceum extract and Agave equilana stem extract are compounded in appropriate proportions, the inhibitory effect of the composition on hyaluronidase is more obvious.

[0073] Effect test example 3 The compositions obtained in Examples 1 to 4 and Comparative Examples 1 and 7 were tested as follows: 1. 140 volunteers aged between 20 and 45 were selected. All of them were capsaicin-positive sensitive skin people. The 140 volunteers with sensitive skin were randomly divided into 7 groups. After daily cleaning, they used the composition for full-face massage. After 28 days, dermatologists scored the full-face skin condition of the sensitive skin volunteers according to the requirements of skin adverse reaction results described in the "Technical Specifications for Safety of Cosmetics-2015 Edition", and counted the number of people with irritation reactions (such as erythema, papules, blisters, edema, etc.).

[0074] 2. 140 volunteers aged between 20 and 45 were selected and randomly divided into 7 groups. After daily cleansing, they used the composition for full face massage for 2 consecutive months (60 days).

[0075] (1) The skin moisture content of the volunteers was measured using a skin moisture meter (CM825), and the increase in skin moisture content was calculated according to the following formula: Increase in skin moisture content = skin moisture content after use - skin moisture content before use.

[0076] (2) The skin elasticity of the volunteers was measured using a skin elasticity meter, and the skin elasticity improvement rate was calculated according to the following formula: Skin elasticity improvement rate = (skin elasticity parameter after use - skin elasticity parameter before use) * 100%.

[0077] The test results are shown in Table 2.

[0078] Table 2 Group Number of stimulus responses Increase in skin moisture content Skin elasticity improvement rate Example 1 0 7.4% 16.2% Example 2 0 6.3% 13.2% Example 3 0 4.1% 9.3% Example 4 2 5.4% 7.6% Comparative Example 1 0 3.1% 4.1% Comparative Example 7 0 3.4% 2.3% Comparative Example 8 5 4.6% 6.8% It can be seen from Table 2 that compared with Examples 3-4 and Comparative Examples 1 and 7-8, the compositions provided by Examples 1-2 of the present invention are mild and non-irritating, suitable for sensitive skin, and have better moisturizing and repairing anti-aging effects.

[0079] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. A skin-revitalizing, nourishing and soothing composition, characterized in that: The invention comprises the following components in percentage by mass: 0.5-5% plant extract, 5-6.9% moisturizer, 0.01-0.05% chelating agent, 0.05-0.25% thickener, 0.01-0.1% heparin sodium, 16-28% emollient, 0.1-0.5% antioxidant, 4.5-7% emulsifier, 1.1-1.3% pH adjuster, 0.1-0.5% preservative and balance water. The plant extract comprises agave stem extract, kava root extract, Centella asiatica extract, gentian root extract and iris extract. The mass ratios of kava root extract, Centella asiatica extract, gentian root extract, iris extract and agave stem extract are (1-4):(0.5-2):(3-6):(0.5-2):

10.

2. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The mass ratio of the kava root extract, the Centella asiatica extract, the gentian root extract, the white osmanthus extract and the agave stem extract is (2-3): (1-1.5): (4-5): (1-1.5):

10.

3. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The invention comprises the following components in percentage by weight: 2-4% plant extract, 6-6.5% moisturizing agent, 0.02-0.04% chelating agent, 0.16-0.2% thickening agent, 0.02-0.05% heparin sodium, 20.4-21.8% emollient, 0.12-0.3% antioxidant, 5.1-6.3% emulsifier, 1.1-1.3% pH adjusting agent, 0.1-0.5% preservative and the balance water.

4. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The thickening agent includes carbomer.

5. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The moisturizing agent includes at least one of sodium hyaluronate, betaine, glycerol, glyceryl polyether-26, allantoin, panthenol, trehalose, dipropylene glycol, and butylene glycol.

6. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The emollient includes at least one of sea buckthorn oil, jojoba oil, cyclopentasiloxane, cyclohexasiloxane, caprylic / capric triglyceride, avocado butter, isopropyl myristate, ethylhexyl palmitate, hydrogenated polyisobutene, macadamia seed oil, hydrogenated polydecene, isodecyl neopentanoate, and dicaprylyl carbonate.

7. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The emulsifier includes at least one of cetearyl alcohol, cetearyl glucoside, glyceryl stearate, and PEG-100 stearate.

8. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The pH regulator is a compound of arginine and water, wherein the mass percentage of arginine in the composition is 0.1-0.3%.

9. The skin revitalizing, nourishing and soothing composition according to claim 1, characterized in that: The antioxidant includes tocopherol acetate.

10. A method for preparing the skin activating, nourishing and soothing composition according to any one of claims 1 to 9, characterized in that: The steps include: S1, mixing water, moisturizing agent, chelating agent, thickener and heparin sodium, heating to 82-85°C, stirring and dissolving completely to obtain P1 phase; S2, mixing the emulsifier, emollient and antioxidant and heating them to 78-82°C to completely dissolve them to obtain phase P2; S3. Add phase P2 to phase P1 under stirring, homogenize for 5-8 minutes until completely dissolved, keep warm for 15-20 minutes, and defoam. S4, cooling the mixture after defoaming in step S3 to 64-66°C, adding pH regulators in sequence, stirring evenly, and then cooling to a temperature not higher than 45°C, adding plant extracts and preservatives in sequence, and stirring evenly; S5. Adjust the temperature of the mixture obtained in step S4 to 38-40° C., filter, discharge, and allow to stand to obtain the skin revitalizing, moisturizing, and soothing composition.

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