Application of fish maw and colla corii asini composition in preparation of anti-aging product

Abstract

The invention relates to application of a fish maw and colla corii asini composition in preparation of anti-aging products, and belongs to the technical field of health-care products. The mass ratio of the fish maw to the colla corii asini in the fish maw and colla corii asini composition is (1-2): 1, and the colla corii asini is Dongshan colla corii asini powder. The fish maw and colla corii asini composition has the advantages that the anti-oxidation level, the collagen metabolism level and the cell function level are mutually cooperated, the anti-oxidation capability is obviously improved, the dermis layer is delayed to be thinned, the skin elasticity is effectively improved, and the anti-aging effect is achieved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of health care products, and particularly relates to application of a composition of fish maw and donkey-hide gelatin in preparation of anti-aging products. BACKGROUND

[0002] In recent years, with the progress of biotechnology and the improvement of social and economic level, the rapid development of skin cosmetic research has been promoted, especially the development of cosmetic raw materials with multiple functions such as moisturizing, inhibiting melanin production and delaying aging. Skin aging is a multifactorial phenomenon driven by endogenous biological programmed decline and exogenous oxidative damage. In the process of natural aging, the I collagen fibers in the reticular layer of the dermis gradually decrease, leading to impaired biomechanical function of the dermal-epidermal junction, and clinical manifestations of decreased skin elasticity and elastosis.

[0003] 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) and 2,2-diazene-di (3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt (ABTS) free radical scavenging capacity and total antioxidant capacity (T-AOC) are key biological markers for evaluating the potential of nutritional matrix to delay aging. In addition, the steady-state imbalance between free radical generation and endogenous clearance mechanism can exacerbate oxidative stress and trigger deterioration of cell ultrastructure and function. The depletion of key antioxidants (such as glutathione, GSH) and antioxidant enzymes (such as superoxide dismutase, SOD) can weaken the cell defense ability and accelerate functional degradation. Fibroblasts, as the main cells of collagen synthesis in the dermis, secrete about 90% of the total collagen as type I collagen (ColI), which is essential for maintaining skin elasticity and delaying aging. Studies have shown that enhancing antioxidant capacity, up-regulating antioxidant enzyme activity and removing excess free radicals can reduce cellular oxidative damage and maintain tissue homeostasis, thereby delaying skin aging.

[0004] Donkey-hide gelatin is a solid gelatinous substance made from boiled and concentrated donkey skin, rich in protein, polypeptide, amino acid, trace element and active polysaccharide components, with biological activities such as hemostasis, anti-fatigue, anti-tumor, immune regulation and anti-aging. Wang et al. found that donkey-hide gelatin can scavenge free radicals, enhance antioxidant enzyme activity and regulate the expression of aging-related genes.

[0005] Eel collagen contains fibrous collagen peptides, essential amino acid polymers, omega-3 eicosapentaenoic acid complexes, zinc / selenium metal enzymes, hyaluronic acid glycosaminoglycans, and sulfated polysaccharide conjugates, and other bioactive ingredients, which are used as multifunctional nutrients for immune regulation and tissue regeneration in traditional pharmacology. Studies have shown that eel collagen has antioxidant, anti-aging, anti-fatigue, wound healing and skin elasticity improving effects, and its collagen and hyaluronic acid have significant effects on delaying skin aging. Yang et al. found that eel collagen peptides have strong scavenging capacity for DPPH, hydroxyl radical and ABTS radical. Ren et al. found that eel enzymatic hydrolysate can improve cell viability, inhibit lipid peroxidation and reduce active oxygen (ROS) accumulation.

[0006] However, the anti-aging effect of single eel or eel collagen is limited, and it is still necessary to improve the anti-aging effect by accurately matching the two kinds of collagen sources. SUMMARY

[0007] The purpose of the present application is to provide an application of a eel and eel collagen composition in the preparation of an anti-aging product, wherein the mass ratio of eel and eel collagen in the eel and eel collagen composition is (1-2):1.

[0008] In an embodiment of the present application, the anti-aging is to enhance the antioxidant capacity of the body, improve the skin morphology, promote cell migration and vitality recovery, and stimulate collagen production.

[0009] In an embodiment of the present application, the preparation of the eel and eel collagen composition comprises the following steps: eel dry is cooked in a steamer for 8-10 min, and after cooling, the eel dry is soaked in cold water for 10-14 h, and then crushed after the soaking is completed; then add eel collagen, stir evenly, and obtain the eel and eel collagen composition.

[0010] In an embodiment of the present application, the eel collagen is Donge Eel Collagen Powder, which has an antioxidant effect.

[0011] In an embodiment of the present application, the Donge Eel Collagen Powder is a powder prepared by decocting and concentrating the dried or fresh skin of Equus asinus L., a horse.

[0012] In an embodiment of the present application, the anti-aging product is used once a day to three times a day, and each time 2 g to 4 g.

[0013] In an embodiment of the present application, the anti-aging product is a food.

[0014] In an embodiment of the present application, the food is one or more of a beverage, a paste food, a preserved fruit food, and a pastry food.

[0015] In an embodiment of the present application, the anti-aging product is a health product.

[0016] In an embodiment of the present application, the dosage form of the health product is selected from one or more of tablets, capsules, soft capsules, granules, pills and oral liquids.

[0017] The technical solution of the present application has the following advantages compared with the prior art:

[0018] (1) The composition of dried flowered donkey-hide gelatin and donkey-hide gelatin in the present application realizes synergistic anti-aging through three mechanisms: first, in the aspect of antioxidant, the active ingredients such as small molecule peptides and polysaccharides in donkey-hide gelatin can scavenge free radicals, and the small molecule peptides can activate the Nrf2 signaling pathway to induce the body to synthesize antioxidant enzymes such as glutathione peroxidase, significantly improve the activity of SOD, and reduce the damage of oxidative stress to immune cells; and the zinc and selenium rich in dried flowered donkey-hide gelatin are the core cofactors of SOD and GSH-Px, which together construct an efficient antioxidant network and form an antioxidant positive cycle. Secondly, in the aspect of collagen metabolism, donkey-hide gelatin stimulates the production of type I collagen by activating the TGF-β / Smad signaling pathway, and dried flowered donkey-hide gelatin provides synthesis raw materials such as hydroxyproline, so that the amount of collagen synthesis is significantly increased; at the same time, the two synergistically inhibit matrix metalloproteinases to reduce collagen degradation and restore the thickness of the dermal layer. Finally, in the aspect of cell function: the hyaluronic acid in dried flowered donkey-hide gelatin repairs the extracellular matrix, and the donkey-hide gelatin peptides promote the migration of fibroblasts, the migration rate is greatly improved, and tissue regeneration is accelerated and aging is delayed. The composition fully plays the synergistic effect of the two in supplementing collagen and antioxidant, and significantly enhances the anti-aging effect.

[0019] (2) The composition of dried flowered donkey-hide gelatin and donkey-hide gelatin in the present application can significantly improve the antioxidant capacity, delay the thinning of the dermal layer, effectively improve the skin elasticity, and achieve the effect of anti-aging. BRIEF DESCRIPTION OF DRAWINGS

[0020] In order to make the content of the present application more easily understood, the present application will be further described in detail below according to specific embodiments of the present application and in conjunction with the drawings, in which:

[0021] Figure 1 Table 1 is the in vitro antioxidant capacity test results of the ACC group, the LFM group, the HFM group, the LAF group and the HAF group in Example 1 of the present application; wherein different letters indicate significant differences between groups (P<0.05);

[0022] Figure 2 Table 2 is the influence of the ACC group, the LFM group, the HFM group, the LAF group and the HAF group on the organ coefficients of rats in Example 2 of the present application;

[0023] Figure 3Effects of the ACC group, the LFM group, the HFM group, the LAF group and the HAF group on the SOD activity (A) and the MDA content (B) of the skin of rats in Example 3 of the present application;

[0024] Figure 4 HE staining photographs of different groups in Example 4 of the present application are shown in the following table, wherein (A) is NC, (B) is AM, (C) is ACC, (D) is LFM, (E) is HFM, (F) is LAF, and (G) is HAF;

[0025] Figure 5 Polarized light photographs of slices dyed with saffron red in different groups in Example 4 of the present application are shown in the following table, wherein (A) is NC, (B) is AM, (C) is ACC, (D) is LFM, (E) is HFM, (F) is LAF, and (G) is HAF;

[0026] Figure 6 Effects of the ACC group, the LFM group, the HFM group, the LAF group and the HAF group on cell viability in Example 5 of the present application;

[0027] Figure 7 Effects of the LAF group on the migration of HFF-1 cells in Example 6 of the present application are shown in the following table, wherein (A) is the microscope observation of the KB group cultured for 0 h, (B) is the microscope observation of the LAF group cultured for 0 h, (C) is the microscope observation of the cells cultured for 12 h, (D) is the microscope observation of the cells cultured for 12 h after adding the LAF group to the culture medium, and (E) is the cell migration rate of each group after being cultured for 12 h;

[0028] Figure 8 Effects of the LAF group on the synthesis of collagen of HFF-1 cells in Example 7 of the present application. DETAILED DESCRIPTION

[0029] The present application will be further described in conjunction with the drawings and specific examples so that those skilled in the art can better understand the present application and implement it, but the examples are not intended to limit the present application.

[0030] In the present application, unless otherwise specified, the SD rats used in the examples of the present application are provided by Spafas (Suzhou) Biotechnology Co., Ltd. (license number SCXK (Jing) 2022-0006).

[0031] In the present application, unless otherwise specified, the donkey-hide gelatin powder used in the examples of the present application is Donge Donkey-hide Gelatin Powder, which is purchased from Donge Donkey-hide Gelatin Co., Ltd. and has a product batch number of 20230403.

[0032] In the present application, unless otherwise specified, the flower gelatin used in the examples of the present application is purchased from Donge Donkey-hide Gelatin Co., Ltd.

[0033] In the present application, the basal feed used in the embodiments of the present application is purchased from Jiangsu Cooperation Pharmaceutical Bioengineering Co., Ltd. unless otherwise specified.

[0034] In the present application, the preparation of the dried flower and donkey-hide gelatin composition used in the embodiments of the present application includes the following steps: rinsing the dust and impurities on the surface of the dried flower with running water, softening the dried flower in a steamer for 9 min, soaking the dried flower in cold water overnight (about 12 h) after cooling, taking out the dried flower and draining the water after the soaking is completed, then crushing, adding a certain amount of donkey-hide gelatin powder after passing through an 80-mesh screen, and mixing uniformly by shaking to obtain the dried flower and donkey-hide gelatin composition.

[0035] Example 1: In vitro antioxidant capacity determination

[0036] Test method: The antioxidant capacity of the ACC group, the LFM group, the HFM group, the LAF group and the HAF group was detected by a kit, specifically including: DPPH free radical scavenging capacity (purchased from Nanjing Jiancheng, item number A153-1-1), ABTS free radical scavenging capacity (purchased from Beijing Solabio, item number BC4770) and T-AOC total antioxidant capacity (purchased from Nanjing Jiancheng, item number A015-3-1), and the test results are shown in Table 1. Figure 1

[0037] From the results shown in Table 1, it can be seen that the antioxidant capacity of the LAF group and the HAF group is significantly higher than that of the ACC group, the LFM group and the HFM group, and the antioxidant capacity of the LAF group is significantly higher than that of the HAF group. Figure 1 ​It can be seen that the DPPH radical scavenging ability is in the order of HAF group > HFM group > LAF group > LFM group > ACC group; the DPPH radical scavenging ability of the HAF group is 79.93 ± 4.35%, which is significantly higher than that of the ACC group (63.59 ± 1.70%) and the HFM group (71.60 ± 4.79%). The ABTS radical scavenging ability is in the order of ACC group > LAF group > HAF group > LFM group > HFM group; the ABTS scavenging ability of the ACC group is the strongest (78.77 ± 3.56%) (P < 0.05), and the ABTS scavenging ability of the HFM group is the weakest (24.67 ± 2.13) (P < 0.05). Compared with the LFM group and the HFM group, the ABTS scavenging ability of the LAF group and the HAF group is significantly enhanced (P < 0.05), and the content of the flower jelly is negatively correlated with the antioxidant property (P < 0.05), which indicates that the donkey-hide gelatin dominates the ABTS radical scavenging in the composition. The combination of the donkey-hide gelatin and the flower jelly can significantly improve the T-AOC, and the total antioxidant capacity of the T-AOC is in the order of LAF group > HAF group > LFM group > HFM group > ACC group; the T-AOC of the LAF group is the highest (1.47 ± 0.05 U / mL) (P < 0.05), and there is no significant difference in the T-AOC of the LFM group, the HFM group and the ACC group (P > 0.05), which indicates that the combination of the donkey-hide gelatin and the flower jelly can significantly improve the T-AOC. It can be seen that the in-vitro antioxidant capacity of the flower jelly and the donkey-hide gelatin composition is stronger than that of the single donkey-hide gelatin and the single flower jelly.

[0038] Example 2 Effects on the organ indexes of SD rats

[0039] Test method: 60 SPF 9-month-old healthy female SD rats were pre-fed in the quarantine room of the barrier facility for 5 days, and then the animals were randomly divided into 6 groups according to the animal weight, namely the model group (AM, basic feed), the donkey-hide gelatin group (ACC, 0.25 g / kg BW), the flower jelly low-dose group (LFM, 0.25 g / kg BW), the flower jelly high-dose group (HFM, 0.5 g / kg BW), the donkey-hide gelatin + flower jelly low-dose group (LAF, 0.25 g / kg BW + 0.25 g / kg BW) and the donkey-hide gelatin + flower jelly high-dose group (HAF, 0.25 g / kg BW + 0.5 g / kg BW), 10 animals in each group. The addition amount of each group was calculated according to the rat food intake of 80 g / kg, the composition was mixed with the basic feed, and then the solid feed was obtained by mechanical compression, and the rats were continuously fed by the feed mixing method for 60 days. Ten 3-month-old healthy female SD rats were purchased one week before the last feeding as the adult control group (NC, basic feed). The water and food intake of the rats in each group was not controlled during the whole feeding period, and the rat food intake, water intake and body weight were recorded every week. The SD rats were fasted for 16 h after the last feeding, weighed and sacrificed, and the heart, thymus, liver, lung, spleen and kidney were weighed to calculate the organ index (the ratio of the organ weight of the experimental animal to the body weight), and the results are as follows Figure 2As shown.

[0040] from Figure 2 As can be seen, compared with the NC group, the thymus, liver, kidney, and spleen indices of SD rats in the AM group were significantly reduced (P<0.05), indicating that the aging model has been successfully established. Compared with the AM group, the indices of all organs in the SD rats of the test substance group were increased, but there was no significant difference (P>0.05), indicating that donkey-hide gelatin and fish maw can maintain the body's immune function and play a certain anti-aging role. The mortality rate of all groups was 0%, indicating that donkey-hide gelatin and fish maw have a certain degree of safety for consumption.

[0041] Example 3: Effects of SOD activity and MDA content in the skin of SD rats

[0042] Experimental Methods: Based on Example 2, after euthanizing SD rats, the backs of the rats were electrically shaved, and the skin from the backs of the SD rats was stored at -80°C. After thawing, the skin from the backs of the SD rats was uniformly sampled, and the activity of superoxide dismutase (SOD) (purchased from Nanjing Jiancheng, catalog number A001-3-1) and the content of malondialdehyde (MDA) (purchased from Nanjing Jiancheng, catalog number A003-1-1) were determined using a kit. The results are as follows: Figure 3 As shown.

[0043] from Figure 3 As can be seen, compared with the NC group, the SOD activity in the skin of SD rats in the AM group was significantly reduced (P<0.05), while the MDA content was significantly increased (P<0.05), indicating that the aging model was successfully established. Compared with the AM group, the SOD activity in the skin of SD rats in the test group was significantly increased (P<0.05), with the highest SOD activity in the LAF, HAF, and HFM groups (P<0.05), indicating that the test group can improve the SOD activity in the skin of SD rats, scavenge free radicals in the body, and alleviate the level of oxidative stress. Meanwhile, the MDA content in the skin of SD rats in the test group was significantly lower than that in the AM group (P<0.05), indicating that both donkey-hide gelatin and fish maw help improve the oxidative state of the skin of aging SD rats and enhance antioxidant capacity, with the HFM and HAF groups showing particularly significant effects.

[0044] Example 4: Effects of dermal thickness and collagen in SD rat skin

[0045] Experimental method: Based on Example 2, after euthanizing SD rats, the hair on the back of the SD rats was electrically shaved, and a 1cm section was taken. 2Back skin was fixed in 4% paraformaldehyde. The fixed skin tissue was first embedded in paraffin and sectioned. After dewaxing and hydration, the sections were stained with hematoxylin and eosin (HE) and Sirius red, respectively. The dehydrated sections were then cleared with xylene and mounted with neutral resin. HE-stained sections were observed under a regular optical microscope, while Sirius red-stained sections were observed under a polarized light microscope. Sirius red binds to collagen fibers of different diameters, exhibiting different colors under polarized light: type I collagen appears red or yellow under polarized light, while type III collagen appears green. The content of type I and type III collagen fibers in the back skin of SD rats was analyzed using ImageJ software. The results are as follows: Figures 4-5 As shown.

[0046] from Figure 4 As can be seen, when stained with hematoxylin and eosin (HE), the dermis appears red, subcutaneous fat appears white, and the yellow area represents muscle. The NC group SD rats had intact skin structure, less subcutaneous fat, and a thicker dermis, indicating good overall skin condition. Compared to the NC group, the AM group SD rats, while having intact skin structure, had increased subcutaneous fat and reduced dermal thickness. When dermal thickness is significantly reduced, the ability of collagen to support the skin structure weakens, leading to decreased skin elasticity and gradual sagging. Compared to the AM group, the test substance group SD rats showed significant improvement in skin morphology, with more intact skin structure, reduced subcutaneous fat, and increased dermal thickness. This indicates that continuous intake of donkey-hide gelatin and fish maw for a period of time can significantly improve dermal thinning caused by aging, with the LAF group showing the best effect.

[0047] from Figure 5 It can be seen that the NC group SD rats had high levels of collagen fibers in their skin, which were densely packed, with both coarse red fibers and fine green fibers being quite prominent. Compared with the NC group, the AM group SD rats showed a significant reduction in collagen fibers, especially fine green fibers (P<0.05). Compared with the AM group, the HFM, LAF, and HAF groups SD rats showed a significant increase in dermal collagen fiber density (P<0.05), with the LAF group showing the greatest increase. This indicates that the LAF group can promote collagen biosynthesis in dermal fibroblasts, thereby enhancing dermal elasticity and tensile strength, and helping to slow down the skin aging process.

[0048] Effect of Example 5 on cell viability

[0049] Experimental Methods: A simulated digestion solution was obtained from an in vitro simulated digestion experiment (referencing M. Minekus's work) to assess the effect of the test substance on cell viability. Fibroblast proliferation was detected using a Cell Counting Kit-8 (CCK-8). Cells were cultured at a rate of 2 × 10⁶ cells / year. 5cells / mL were seeded in 96-well plates (100 μL per well) and divided into six groups: KB group (blank control group), ACC group, LFM group, HFM group, LAF group and HAF group. After 24 h of incubation under standard conditions (37 °C, 5% CO2, humidified environment), the cells were treated with serially diluted digest (0, 0.25 mg / mL, 0.5 mg / mL, 1 mg / mL, 2 mg / mL, 5 mg / mL, 10 mg / mL, 20 mg / mL final concentration) for 24 h in high glucose DMEM medium containing 10% fetal bovine serum (FBS). Then, the original medium was replaced with serum-free DMEM medium and incubation was continued for 4 h, 10 μL CCK-8 reagent was added to each well and incubated for 1 h. The absorbance at 450 nm was measured using a microplate reader. The dose-effect curve was plotted based on the cell survival rate of the blank control group, and the results are shown in Figure 6 .

[0050] As can be seen from Figure 6 , the cell viability of the LAF group was the highest (164.78%-343.73%) (P < 0.05), which was consistent with the previous in vitro antioxidant activity and animal experiment results. Therefore, the LAF group was selected as the optimal concentration in the subsequent scratch test and type I collagen secretion difference test.

[0051] Example 6 Effect on the migration ability of HFF-1 cells

[0052] Test method: Based on Example 5, the migration ability of fibroblasts was evaluated by scratch healing test, and the experiment was carried out under the condition of inhibiting proliferation (medium containing 1% FBS); HFF-1 cells were seeded in 12-well plates at a density of 1 x 10 5 cells / mL and cultured to complete confluence (100%); a sterile 200 μL pipette tip was used to draw a uniform straight line scratch on the monolayer cells, and then washed with PBS three times to remove cell debris; the LAF group was added with 1% FBS-DMEM medium containing LAF, and the KB group was only added with 1% FBS-DMEM medium; at 0 h and 12 h after scratching, time-lapse imaging was performed using a phase contrast microscope (20x); Image J (NIH) was used for quantitative analysis of wound closure, and the migration rate was calculated, i.e. the percentage of wound area reduction at 12 h after treatment relative to the baseline (0 h), and the results are shown in Figure 7 .

[0053] As can be seen from Figure 7 , compared with the KB group, the LAF group significantly improved the migration distance and density of HFF-1 cells, and the migration rate was increased by 38.54%, indicating that LAF can effectively promote the growth and movement of fibroblasts.

[0054] Example 7 Effect on collagen content of cells

[0055] Test method: Based on Example 5, primary HFF-1 cells were seeded in 96-well cell culture plates at a density of 5 x 10 4 cells / mL (100 μL per well) and incubated for 24 h under standard conditions (37°C, 5% CO2, humidified environment); after the initial culture, the medium was replaced with DMEM containing 10% FBS and LAF was added; after 24 h, the supernatant was collected and the collagen type I content was quantified using an enzyme-linked immunosorbent assay (ELISA) kit, and the results are shown in Figure 8

[0056] From Figure 8 it can be seen that the collagen content of the control group (without the addition of LAF) after 24 h of incubation was 384.12 ± 10.70 ng / mL; after treatment with 0.5 mg / mL, 1 mg / mL, 2.5 mg / mL, 5 mg / mL and 10 mg / mL of LAF, the collagen levels were significantly increased to 473.32 ± 30.95 ng / mL, 496.52 ± 42.01 ng / mL, 535.83 ± 63.28 ng / mL, 641.58 ± 64.70 ng / mL and 506.13 ± 52.44 ng / mL, respectively, which was 23.22%, 29.26%, 39.50%, 67.03% and 31.76% higher than the control group, respectively. This shows that the LAF group can significantly stimulate collagen synthesis. In addition, 5 mg / mL of LAF treatment induced the highest collagen type I synthesis in HFF-1 cells (P < 0.05), indicating that this is the optimal concentration for enhancing collagen production in HFF-1 cells. In summary, the LAF group can upregulate collagen production in HFF-1 cells, with the most significant effect at a concentration of 5 mg / mL.

[0057] Obviously, the above examples are merely examples for the sake of clarity and are not limiting of the embodiments. Other different forms of changes or variations can be made by those of ordinary skill in the art on the basis of the above description. It is not necessary or possible to exhaust all embodiments. The obvious changes or variations derived therefrom are still within the scope of protection of the present invention.​

Claims

1. Use of a combination of eel skin and donkey-hide gelatin in the preparation of an anti-aging product, characterized in that, The mass ratio of flower jelly and donkey-hide gelatin in the flower jelly and donkey-hide gelatin composition is (1-2):

1.

2. Use of the composition of dried placenta and donkey-hide gelatin according to claim 1 in the preparation of anti-aging products, characterized in that, The anti-aging is to enhance the body's antioxidant capacity, improve skin morphology, promote cell migration and vitality recovery, and stimulate collagen production.

3. Use of the composition of dried placenta and donkey-hide gelatin according to claim 1 in the preparation of anti-aging products, characterized in that, The preparation of the flower jelly and donkey-hide gelatin composition comprises the following steps: The flower jelly dry is cooked in a steamer for 8-10 minutes, and after cooling, the flower jelly dry is soaked in cold water for 10-14 hours. After the soaking is completed, the flower jelly dry is crushed. Then, the donkey-hide gelatin is added, and the mixture is stirred uniformly to obtain the flower jelly and donkey-hide gelatin composition.

4. Use of the composition of dried placenta and donkey-hide gelatin according to claim 1 in the preparation of anti-aging products, characterized in that, The donkey-hide gelatin is Dong'e donkey-hide gelatin powder.

5. Use of the composition of dried placenta and donkey-hide gelatin according to claim 4 for the preparation of anti-aging products, characterized in that, The Dong'e donkey-hide gelatin powder is a powder prepared by decocting and concentrating the dried or fresh skin of Equus asinus L.

6. Use of the composition of dried placenta and donkey-hide gelatin according to claim 1 in the preparation of anti-aging products, characterized in that, The anti-aging product is used once to three times a day, and each time 2-4 g.

7. Use of the composition of isinglass and donkey-hide gelatin according to claim 1 in the preparation of anti-aging products, characterized in that, The anti-aging product is a food.

8. Use of the composition of dried placenta and donkey-hide gelatin according to claim 7 for the preparation of anti-aging products, characterized in that, The food is one or more of a beverage, a paste food, a preserved fruit food, and a cake food.

9. Use of the composition of dried placenta and donkey-hide gelatin according to claim 1 in the preparation of anti-aging products, characterized in that, The anti-aging product is a health product.

10. Use of the composition of isinglass and donkey-hide gelatin according to claim 9 for the preparation of anti-aging products, characterized in that, The dosage form of the health product is selected from one or more of a tablet, a capsule, a soft capsule, a granule, a pill, and an oral liquid.

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