A multi-target composition for promoting skin water retention, its preparation method and medical and cosmetic applications

Abstract

The present application relates to the field of medical and cosmetic technology, and particularly relates to a multi-target composition for promoting skin water-locking and moisturizing, and a preparation method and medical and cosmetic application thereof. The composition comprises hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava-kava extract, oat extract, mulberry leaf extract, icodione, glycerol glucoside, sodium hyaluronate, allantoin, butanediol and water. The present application prepares the composition by specific collocation of different raw materials, can promote the generation of filaggrin, tight junction protein and endogenous natural moisturizing factor, enhance the epidermal hydration and skin water-locking and moisturizing effect, promote the expression of water channel protein AQP3, strengthen the water transport in skin (transfer to water-deficient parts) and repair the skin ability. The composition is applied in skin medicine, can accelerate the skin barrier repair; is applied in skin cosmetics, realizes the endogenous water-locking and moisturizing from multi-target, improves the skin barrier, and makes the skin persistent moisturizing, nourishing, full, and elastic.

Description

Technical Field

[0001] This invention relates to the fields of medical and cosmetic technology, and in particular to a multi-target composition for promoting skin hydration and moisturization, its preparation method, and its medical and cosmetic applications. Background Technology

[0002] The skin is the largest organ in the human body and the first line of defense between the body and the external environment. Maintaining the skin's moisture balance is crucial for its normal function. Lack of moisture leads to a reduction in the skin's natural moisturizing factors, resulting in dry, rough, and inelastic skin. Adequate hydration helps maintain skin's moisture level, improves skin texture, and makes it softer and smoother.

[0003] Traditional quick moisturizing solutions typically involve exogenously replenishing the stratum corneum with moisture or reducing transepidermal water loss. Ingredients that quickly replenish the stratum corneum include humectants, hydrating agents, and occlusive agents. These substances can rapidly restore the skin's ability to trap and retain water. For example, glycerin, a commonly used ingredient, has excellent humectant properties, while hyaluronic acid and its derivatives provide moisturizing benefits while offering better skin affinity or excellent film-forming properties to prevent excessive moisture loss. Methods for quickly replenishing the stratum corneum include directly adding natural moisturizing factors (such as sodium pyrrolidone carboxylate) or adding intercellular lipids (such as ceramides). Some plant-derived, water-soluble ceramides can quickly improve skin hydration and are easy to use. Adding lipid-similar ingredients is another fast and effective moisturizing method. These ingredients can directly compensate for deficiencies in the lipid barrier, enhance its function, and form lipid-like structures in the formulation, maintaining the lipid-like structure on the skin.

[0004] While exogenous replenishment of the stratum corneum barrier offers quick results, it doesn't fundamentally or provide long-term improvement. For aging skin, once the stratum corneum barrier is damaged, the repair process is consistently delayed, directly leading to a weakening of the barrier. Only by focusing on the formation process of the stratum corneum barrier and the deeper, moisturizing-related components of the skin, and targeting these components to ensure their healthy expression, can the skin barrier be truly improved, achieving long-lasting hydration.

[0005] Promoting keratin expression can enhance the internal strength of keratinocytes, increase the expression of proteins related to keratin capsule formation (promoting keratin, endothelial proteins, and small proline-rich proteins) and enzymes (transglutaminase), thereby enhancing the toughness and stability of the keratinocyte exterior.

[0006] Natural moisturizing factors (NMFs) in stratum corneum cells are a key component of the stratum corneum's ability to moisturize the skin, and are derived from filaggrin. First, filaggrin precursors are synthesized and aggregate in granular cells, forming numerous transparent protein particles. During the transformation of cells from the granular layer to the stratum corneum, filaggrin precursors are dephosphorylated and hydrolyzed into filaggrin. Filaggrin binds tightly to keratin fibers, intertwining to form a complex that fills newly formed stratum corneum cells, providing them with strength. As the stratum corneum migrates to the skin surface, keratin undergoes strong disulfide bond cross-linking. Once the stratum corneum structure stabilizes, filaggrin unbinds from keratin. This process is often affected by dry external environments. When water activity or relative humidity falls below 95%, proteases in the cells become activated, gradually degrading filaggrin completely into amino acids. This degradation may be due to an increase in ion concentration caused by decreased cell water content; the aggregate of these amino acids is known as the natural moisturizing factor. Some amino acids undergo further changes; for example, glutamic acid forms pyrrolidone carboxylic acid, and histidine forms urocanic acid under the action of histidine enzymes. Urocanic acid's UV absorption function may provide the stratum corneum with natural sun protection. These weakly acidic products also make the stratum corneum weakly acidic, becoming an important component of the stratum corneum's antimicrobial barrier. In the process of filaggrin being converted into natural moisturizing factors, cysteine ​​aspartate protease-14 (Caspase-14) plays a decisive role in its normal degradation. As keratinocytes slowly migrate to near the skin surface, their hydration level decreases to a certain extent. Caspase-14 then marks the sites on the filaggrin that need to be cleaved, thereby directly or indirectly degrading the filaggrin into smaller fragments, generating free amino acids, and ultimately forming natural moisturizing factors.

[0007] A complete moisturizing solution includes not only exogenous replenishment but also targeted endogenous enhancement. Exogenous replenishment of the stratum corneum barrier provides rapid hydration, while endogenously stimulating the skin's self-repair capabilities strengthens the barrier's integrity and moisturizing properties, resulting in hydrated, youthful skin. Summary of the Invention

[0008] To address the shortcomings of the existing technology, the present invention aims to provide a multi-target composition for promoting skin hydration and moisturization, its preparation method, and its medical and aesthetic applications. The composition, by combining hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, ectoine, glyceryl glucoside, sodium hyaluronate, allantoin, and water in specific proportions, improves skin hydration, particularly by promoting filaggrin, tight junction proteins, and the generation of endogenous natural moisturizing factors, thereby enhancing epidermal hydration and improving skin's water retention and moisturizing ability. It also enhances the expression of aquaporin AQP3, strengthening the skin's water transport function and accelerating the transfer of water to dehydrated areas. Simultaneously, it provides excellent soothing and anti-inflammatory effects, effectively strengthening the skin barrier function and benefiting skin damage repair. This composition can accelerate skin barrier repair when applied in dermatology; when applied in cosmetic dermatology, it can achieve endogenous water retention and moisturization from multiple targets, improve the skin barrier, and keep the skin moisturized, hydrated, plump, and elastic for a long time. It can effectively inhibit the stimulation of the skin by exogenous or endogenous inflammatory factors, and provide a highly effective soothing and anti-inflammatory effect.

[0009] This patent discloses a multi-target composition for promoting skin hydration and moisture retention, characterized by comprising hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava pepper extract, oat extract, mulberry leaf extract, ectoine, glyceryl glucoside, sodium hyaluronate, allantoin, butylene glycol, and water.

[0010] Further, according to parts by weight, the composition comprises: hexapeptide-9 0.00001-0.2 parts, tripeptide-1 0.00001-0.2 parts, carnosine 0.00001-0.2 parts, ergothioneine 0.00001-1 part, kava pepper extract 0.00001-2 parts, oat extract 0.00001-2 parts, mulberry leaf extract 0.00001-2 parts, ectoine 0.001-2 parts, glyceryl glucoside 0.0001-3.5 parts, sodium hyaluronate 0.001-1 part, allantoin 0.0001-0.5 parts, butylene glycol 0.5-3 parts, and water 5-150 parts.

[0011] Furthermore, the amount of hexapeptide-9 in the composition can be any value and range of 0.0001, 0.001, 0.01, 0.05, 0.1, or 0.2 parts.

[0012] Furthermore, the amount of tripeptide-1 in the composition can be any value and range of 0.0001, 0.001, 0.01, 0.05, 0.1, or 0.2 parts.

[0013] Furthermore, the amount of carnosine in the composition can be any value and range of 0.0001, 0.001, 0.01, 0.05, 0.1, or 0.2 parts.

[0014] Furthermore, the amount of ergothionein in the composition can be any value and range of 0.0001, 0.001, 0.01, 0.05, 0.1, 0.2, or 0.5 parts.

[0015] Furthermore, the amount of kava pepper extract in the composition can be any value and range of 0.001, 0.01, 0.1, 0.5, 0.8, 1, or 1.5 parts.

[0016] Furthermore, kava pepper extract requires the use of its roots as raw material and extraction with an aqueous ethanol solution of 25-40 wt% for better results.

[0017] Furthermore, the preparation method of kava pepper extract includes: crushing the roots of kava pepper, adding 10-30 times the mass of a 25-40wt% ethanol aqueous solution, heating and stirring at 40-65℃ for 4-10 hours, centrifuging, taking the supernatant and freeze-drying to obtain kava pepper extract, which is more suitable for use in the composition of the present invention.

[0018] Furthermore, the amount of oat extract in the composition can be any value and range of 0.001, 0.01, 0.1, 0.5, 0.8, 1, or 1.5 parts.

[0019] Furthermore, oat extract requires extraction using oat grains as raw material and hot water (e.g., 55-70°C).

[0020] Furthermore, the preparation method of oat extract includes: crushing oat grains, adding 15-40 times the weight of water, heating and stirring at 55-70℃ for 5-10 hours, centrifuging, taking the supernatant and freeze-drying to obtain oat extract.

[0021] Furthermore, the amount of mulberry leaf extract in the composition can be any value and range of 0.001, 0.01, 0.1, 0.5, 0.8, 1, or 1.5 parts.

[0022] Furthermore, the mulberry leaf extract requires the leaves as raw material and extraction using an aqueous ethanol solution with a concentration of 10-25 wt%. The extract obtained by this method has better effects and is more suitable for the composition system of the present invention.

[0023] Furthermore, the preparation method of mulberry leaf extract includes: crushing fresh mulberry leaves, adding 10-50 times the mass of a 10-25wt% ethanol aqueous solution, heating and stirring at 35-55℃ for 6-12 hours, centrifuging, taking the supernatant and freeze-drying to obtain mulberry leaf extract.

[0024] Furthermore, to enhance the soothing and anti-inflammatory functions, the mass ratio of kava pepper extract to mulberry leaf extract is 1.5-3:1, and can also be 1.6:1, 1.7:1, 1.8:1, 2:1, 2.2:1, 2.5:1, 2.8:1, 3:1, etc.

[0025] Furthermore, the amount of ectoine in the composition can be any value and range of 0.01, 0.05, 0.1, 0.5, 1, 1.2, or 1.5 parts.

[0026] Furthermore, the amount of glycerol glucoside in the composition can be any value and range of 0.01, 0.05, 0.1, 0.5, 1, 1.2, 1.5, 2, 3, or 3.5 parts.

[0027] Furthermore, the amount of sodium hyaluronate in the composition can be any value and range of 0.01, 0.05, 0.1, 0.2, 0.5, or 1 part.

[0028] Furthermore, the amount of allantoin in the composition can be any value and range of 0.001, 0.01, 0.05, 0.1, 0.2, or 0.5 parts.

[0029] Furthermore, the amount of butanediol in the composition can be any value and range of 0.5, 0.8, 1, 1.2, 1.5, 2, 2.5, or 3 parts.

[0030] Furthermore, the amount of water in the composition can be any value and range of 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 120, or 150 parts.

[0031] Further, according to parts by weight, the composition comprises: 0.001-0.1 parts of hexapeptide-9, 0.001-0.1 parts of tripeptide-10.001-0.1 parts of carnosine, 0.001-0.5 parts of ergothioneine, 0.1-1.8 parts of kava pepper extract, 0.1-1.8 parts of oat extract, 0.1-1.8 parts of mulberry leaf extract, 0.1-1.5 parts of ectoine, 0.1-2 parts of glyceryl glucoside, 0.1-0.5 parts of sodium hyaluronate, 0.001-0.2 parts of allantoin, 0.8-2.5 parts of butylene glycol, and 20-100 parts of water.

[0032] Furthermore, based on its effective role in promoting moisturizing and repairing the skin, the weight ratio of oat extract to ectoine is 1:0.5-1, and can also be 1:0.6, 1:0.7, 1:0.8, 1:0.9, etc.

[0033] Another aspect of this patent discloses a method for preparing the above-mentioned composition, including:

[0034] Step 1: Prepare the raw materials;

[0035] Step 2: Pour water into a container, heat it, then add butylene glycol and stir, then add glyceryl glucoside, sodium hyaluronate and allantoin, stir, and obtain mixture one;

[0036] Step 3: Cool the mixture, add hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava pepper extract, oat extract, mulberry leaf extract, and ectoine, stir, let stand to age, sterilize, and fill to obtain the composition.

[0037] Further, in step 2, the temperature is heated to 40-55°C, and in step 3, the temperature is cooled to 30-35°C.

[0038] Furthermore, in step 3, the aging temperature can be room temperature.

[0039] Another aspect of this patent discloses the application of the above composition in the preparation of medical aesthetic preparations.

[0040] Another aspect of this patent discloses the application of the above composition in the preparation of moisturizing cosmetics.

[0041] Another aspect of this patent discloses the application of the above composition in the preparation of skin-soothing cosmetics.

[0042] Compared to existing technologies, the advancement of this invention lies in:

[0043] This invention utilizes hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava extract, oat extract, mulberry leaf extract, ectoine, glyceryl glucoside, sodium hyaluronate, allantoin, butylene glycol, and water to prepare a composition. This invention, through specific combinations of different raw materials, prepares a composition that promotes the generation of filaggrin, tight junction proteins, and endogenous natural moisturizing factors, enhancing epidermal hydration and skin's water-locking and moisturizing effects; it also promotes the expression of aquaporin AQP3, strengthening the skin's water transport (transfer to dehydrated areas) and skin repair capabilities. In dermatological medicine, this composition can accelerate skin barrier repair; in cosmetic dermatology, it achieves endogenous water-locking and moisturizing from multiple targets, improving the skin barrier and resulting in long-lasting, moisturized, plump, and elastic skin.

[0044] The product prepared by this invention can effectively increase the moisture content of the stratum corneum and reduce the transepidermal water loss rate of the skin; it has excellent moisturizing and water-locking effects and good skin barrier repair capabilities.

[0045] The oat extract and ectoine used in the preparation of the product in this invention synergistically enhance the composition's moisturizing, water-locking, and skin barrier repair capabilities. Furthermore, oat extracts obtained using other extraction methods besides those described in this invention are less effective and cannot impart the superior effects to the product of this invention.

[0046] The product prepared by this invention can effectively inhibit the expression of inflammatory factors IL-6 and TNF-α, and has a good soothing and anti-inflammatory effect. It can also inhibit the skin barrier damage and decreased moisturizing and water-locking ability caused by inflammatory factors.

[0047] This invention utilizes a combination of kava pepper extract and mulberry leaf extract to create a synergistic effect, effectively enhancing the product's anti-inflammatory and soothing properties. Furthermore, the anti-inflammatory effect is even better when the mass ratio of kava pepper extract to mulberry leaf extract is 1.5-3:1.

[0048] This invention uses 25-40 wt% ethanol aqueous solution to extract kava pepper and 10-25 wt% ethanol aqueous solution to extract mulberry leaves, resulting in extracts that enhance the anti-inflammatory and soothing effects of the product.

[0049] The product prepared by this invention has excellent performance, the preparation method is simple, and it has good application prospects. Detailed Implementation

[0050] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. The following description of at least one exemplary embodiment is merely illustrative and is in no way intended to limit the present invention or its application or use. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0051] The raw material information used in the specific embodiments of this invention is as follows:

[0052] Hexapeptide-9: Sichuan Weikeqi Biotechnology Co., Ltd. Carnosine: L-Carnosine, catalog number C106843; Ergothioneine: L-(+)-Ergothioneine, catalog number L134175; Ectoin: catalog number E292674; Glyceryl glucoside: catalog number G768334; Sodium hyaluronate: catalog number S774025; Allantoin: catalog number A101660; Butylene glycol: 1,3-Butylene glycol, catalog number B111016; from Shanghai Aladdin Biochemical Technology Co., Ltd. Tripeptide-1: CAS: 72957-37-0, Shanghai Ketai Biotechnology Co., Ltd.

[0053] I. Product Preparation

[0054] Product 1:

[0055] The preparation method is as follows:

[0056] Step 1: Prepare the raw materials according to the following weight parts: hexapeptide-90.05 parts, tripeptide-10.04 parts, carnosine-0.03 parts, ergothioneine-0.09 parts, kava pepper extract-1.6 parts, oat extract-1.5 parts, mulberry leaf extract-0.9 parts, ectoine-1.2 parts, glyceryl glucoside-0.3 parts, sodium hyaluronate-0.2 parts, allantoin-0.05 parts, butylene glycol-1.5 parts, and water-65 parts; the weight ratio of kava pepper extract to mulberry leaf extract is 2:1; the weight ratio of oat extract to ectoine is 1:0.8.

[0057] The preparation method of kava pepper extract is as follows: the root of kava pepper is crushed, 15 times the mass of 30wt% ethanol aqueous solution is added, heated at 60℃ and stirred at 50rpm for 8 hours, centrifuged at 1000rpm for 0.4 hours, and the supernatant is freeze-dried at -45℃ to a water content of 2.01wt% to obtain kava pepper extract.

[0058] The oat extract was prepared as follows: oat grains were crushed, 30 times the weight of water was added, the mixture was heated at 62°C and stirred at 55 rpm for 9.5 hours, centrifuged at 1000 rpm for 0.3 hours, and the supernatant was freeze-dried at -45°C until the water content was 1.97 wt% to obtain the oat extract.

[0059] The preparation method of mulberry leaf extract is as follows: Fresh mulberry leaves are crushed, and 20 times the mass of 20wt% ethanol aqueous solution is added. The mixture is heated at 48℃ and stirred at 60rpm for 10 hours, centrifuged at 900rpm for 0.3 hours, and the supernatant is freeze-dried at -45℃ until the water content is 1.85wt% to obtain mulberry leaf extract.

[0060] Step 2: Pour water into a container and heat it to 50°C. Then add butylene glycol and stir at 60 rpm for 0.2 hours. Next, add glyceryl glucoside, sodium hyaluronate, and allantoin, and continue stirring at the same speed for 0.3 hours to obtain mixture one.

[0061] Step 3: Cool the mixture to 30°C, add hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava pepper extract, oat extract, mulberry leaf extract, and ectoine, and continue stirring at the same speed for 0.4 hours. Let it stand at room temperature for 1 hour to age, sterilize, and fill to obtain product 1.

[0062] Product 2:

[0063] The preparation method is as follows:

[0064] Step 1: Prepare the raw materials according to the following weight parts: hexapeptide-90.04 parts, tripeptide-10.03 parts, carnosine-0.06 parts, ergothioneine-0.08 parts, kava pepper extract-1.85 parts, oat extract-1.8 parts, mulberry leaf extract-0.65 parts, ectoine-0.9 parts, glyceryl glucoside-0.2 parts, sodium hyaluronate-0.22 parts, allantoin-0.05 parts, butylene glycol-1.6 parts, and water-65 parts; the weight ratio of kava pepper extract to mulberry leaf extract is 2.7:1; the weight ratio of oat extract to ectoine is 1:0.5.

[0065] The preparation method of kava pepper extract is as follows: the root of kava pepper is crushed, 20 times the mass of 35wt% ethanol aqueous solution is added, heated at 60℃ and stirred at 50rpm for 7 hours, centrifuged at 1000rpm for 0.4 hours, and the supernatant is freeze-dried at -45℃ to a water content of 2.09wt% to obtain kava pepper extract.

[0066] The oat extract was prepared as follows: oat grains were crushed, 40 times the weight of water was added, the mixture was heated at 60°C and stirred at 60 rpm for 10 hours, centrifuged at 1200 rpm for 0.3 hours, and the supernatant was freeze-dried at -45°C until the water content was 1.91 wt% to obtain the oat extract.

[0067] The preparation method of mulberry leaf extract is as follows: Fresh mulberry leaves are crushed, and 30 times the mass of a 10wt% ethanol aqueous solution is added. The mixture is heated at 50℃ and stirred at 60rpm for 8.5 hours, centrifuged at 1000rpm for 0.3 hours, and the supernatant is freeze-dried at -45℃ until the water content is 1.93wt% to obtain mulberry leaf extract.

[0068] Step 2: Pour water into a container and heat it to 40°C. Then add butylene glycol and stir at 60 rpm for 0.2 hours. Next, add glyceryl glucoside, sodium hyaluronate, and allantoin and continue stirring at the same speed for 0.4 hours to obtain mixture one.

[0069] Step 3: Cool the mixture to 32°C, add hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava pepper extract, oat extract, mulberry leaf extract, and ectoine, and continue stirring at the same speed for 0.5 hours. Let it stand at room temperature for 1.2 hours, sterilize, and fill to obtain product 2.

[0070] Product 3:

[0071] The only difference from Product 1 is: Step 1: Prepare the raw materials according to the following parts by weight: Hexapeptide - 90.05 parts, Tripeptide - 10.04 parts, Carnosine - 0.03 parts, Ergothioneine - 0.09 parts, Kava Pepper Extract - 1.6 parts, Mulberry Leaf Extract - 0.9 parts, Ectoin - 2.7 parts, Glyceryl Glucoside - 0.3 parts, Sodium Hyaluronate - 0.2 parts, Allantoin - 0.05 parts, Butylene Glycol - 1.5 parts, and Water - 65 parts.

[0072] Product 4:

[0073] The only difference from Product 1 is: Step 1: Prepare the raw materials according to the following parts by weight: Hexapeptide - 90.05 parts, Tripeptide - 10.04 parts, Carnosine - 0.03 parts, Ergothioneine - 0.09 parts, Kava Pepper Extract - 1.6 parts, Oat Extract - 2.7 parts, Mulberry Leaf Extract - 0.9 parts, Glyceryl Glucoside - 0.3 parts, Sodium Hyaluronate - 0.2 parts, Allantoin - 0.05 parts, Butylene Glycol - 1.5 parts, and Water - 65 parts.

[0074] Product 5:

[0075] The only difference from Product 1 is that the oat extract is prepared as follows: oat grains are crushed, 30 times the mass of a 65wt% ethanol aqueous solution is added, heated at 62℃ and stirred at 55rpm for 9.5 hours, centrifuged at 1200rpm for 0.3 hours, and the supernatant is freeze-dried at -45℃ until the water content is 1.97wt% to obtain the oat extract.

[0076] Product 6:

[0077] The only difference from Product 1 is: Step 1: Prepare the raw materials according to the following parts by weight: Hexapeptide - 90.05 parts, Tripeptide - 10.04 parts, Carnosine - 0.03 parts, Ergothioneine - 0.09 parts, Kava Pepper Extract - 2.5 parts, Oat Extract - 1.5 parts, Ectoin - 1.2 parts, Glyceryl Glucoside - 0.3 parts, Sodium Hyaluronate - 0.2 parts, Allantoin - 0.05 parts, Butylene Glycol - 1.5 parts, and Water - 65 parts.

[0078] Product 7:

[0079] The only difference from Product 1 is: Step 1: Prepare the raw materials according to the following parts by weight: Hexapeptide-90.05 parts, Tripeptide-10.04 parts, Carnosine-0.03 parts, Ergothioneine-0.09 parts, Oat Extract-1.5 parts, Mulberry Leaf Extract-2.5 parts, Ectoin-1.2 parts, Glyceryl Glucoside-0.3 parts, Sodium Hyaluronate-0.2 parts, Allantoin-0.05 parts, Butylene Glycol-1.5 parts, and Water-65 parts.

[0080] Product 8:

[0081] The only difference from Product 1 is: Step 1: Prepare the raw materials according to the following parts by weight: Hexapeptide-90.05 parts, Tripeptide-10.04 parts, Carnosine-0.03 parts, Ergothioneine-0.09 parts, Kava Pepper Extract-0.4 parts, Oat Extract-1.5 parts, Mulberry Leaf Extract-2.1 parts, Ectoin-1.2 parts, Glyceryl Glucoside-0.3 parts, Sodium Hyaluronate-0.2 parts, Allantoin-0.05 parts, Butylene Glycol-1.5 parts, and Water-65 parts; the mass ratio of Kava Pepper Extract to Mulberry Leaf Extract is 0.19:1.

[0082] Product 9:

[0083] The only difference from Product 1 is: Step 1: Prepare the raw materials according to the following parts by weight: Hexapeptide-90.05 parts, Tripeptide-10.04 parts, Carnosine-0.03 parts, Ergothioneine-0.09 parts, Kava Pepper Extract-2.2 parts, Oat Extract-1.5 parts, Mulberry Leaf Extract-0.3 parts, Ectoin-1.2 parts, Glyceryl Glucoside-0.3 parts, Sodium Hyaluronate-0.2 parts, Allantoin-0.05 parts, Butylene Glycol-1.5 parts, and Water-65 parts; the mass ratio of Kava Pepper Extract to Mulberry Leaf Extract is 7.3:1.

[0084] Product 10:

[0085] The only difference from Product 1 is the preparation method of Kava pepper extract: The roots of Kava pepper are crushed, and 15 times their weight of a 70wt% ethanol aqueous solution is added. The mixture is heated at 60℃ and stirred at 50rpm for 8 hours, centrifuged at 1000rpm for 0.4 hours, and the supernatant is freeze-dried at -45℃ to a water content of 2.01wt% to obtain Kava pepper extract. The preparation method of mulberry leaf extract is as follows: Fresh mulberry leaves are crushed, and 20 times their weight of an 80wt% ethanol aqueous solution is added. The mixture is heated at 48℃ and stirred at 60rpm for 10 hours, centrifuged at 900rpm for 0.3 hours, and the supernatant is freeze-dried at -45℃ to a water content of 1.85wt% to obtain mulberry leaf extract.

[0086] II. Product Testing

[0087] 1. Transepidermal water loss rate and stratum corneum water content of the skin

[0088] Transepidermal water loss (TEWL) is an important parameter reflecting skin moisture loss and barrier function. The lower the measured value, the better the skin's barrier function and repair ability, and the better its water retention effect. This invention uses a Vapometer, SWL5201, for measurement.

[0089] A higher skin stratum corneum moisture content value indicates a higher moisture content in the stratum corneum, and therefore a better moisturizing effect from the product. This invention uses a Cormeometer, CM825, for testing.

[0090] The testing methods for both products are as follows: Forty female volunteers aged 35-55 with healthy skin were randomly divided into 5 groups of 8 people each. Before using the products, each group of volunteers washed their cheeks and dried them with tissues. Then, the transepidermal water loss (TEWL) and stratum corneum moisture content of the skin on the volunteers' cheeks were tested and the results were recorded. Then, each group of volunteers used products 1-5 of the above products for testing. The usage method was as follows: each group used the corresponding product once at 9:00 and 18:00 every day, applying 1.5g of the product evenly to the face each time, for 2 consecutive months. Three hours after the last use, the TEWL and stratum corneum moisture content of each group's cheeks were tested again and the results were recorded. The transepidermal water loss (TEWL) of the skin at the beginning and after the experiment, as well as the amount and rate of reduction after use compared to before use, were calculated for each group. The stratum corneum moisture content of the skin at the beginning and after the experiment, as well as the amount and rate of increase after use compared to before use, were also calculated for each group. The results are shown in Tables 1 and 2.

[0091] Table 1: Transepidermal water loss (TEWL), increase, and rate of increase before and after use.

[0092]

[0093] Table 2: Skin stratum corneum moisture content (A value), increase, and rate of increase before and after use.

[0094]

[0095] Based on the test results in Table 1-2, it can be seen that the product prepared by the present invention can effectively increase the moisture content of the stratum corneum and reduce the transepidermal water loss rate of the skin; thus, the product of the present invention has excellent moisturizing and water-locking effects and good skin barrier repair capabilities.

[0096] Based on the tests of Products 1 and 3-4 in Tables 1-2, it can be seen that Product 1, obtained by combining oat extract and ectoine, is more effective than Products 3-4, which use only one of the two at the same dosage as Product 1. This demonstrates that the combination of oat extract and ectoine in the preparation of the product in this invention synergistically enhances the moisturizing, water-locking, and skin barrier repair capabilities of the composition. According to the test results of Products 1 and 5, the oat extract obtained using other extraction methods is less effective than that obtained using this method and cannot impart the superior effects of the product of this invention.

[0097] 2. Soothing and anti-inflammatory

[0098] The test aimed to inhibit the release of inflammatory factors in vitro and verify the product's soothing and anti-inflammatory effects.

[0099] RAW264.7 cells were added to each well at a density of 0.5 mL (1.0 × 10⁻⁶). 5Cells (1 mmol / mL) were seeded into 24-well cell culture plates and cultured for 24 h. Cells were then divided into groups. Except for the control group, all other groups had LPS added to a concentration of 1 μg / mL to establish an inflammation model. Specifically, the model group received only LPS to a concentration of 1 μg / mL, while the product 1-2 and 6-10 groups (corresponding to the use of products 1-2 and 6-10) received LPS to a concentration of 1 μg / mL, and the corresponding product for each group was added to a concentration of 0.015 wt%. Cells were incubated at 37°C for another 24 h. The supernatant was then collected, and the expression levels of IL-6 and TNF-α in each group, as well as the reduction in IL-6 and TNF-α levels compared to the model group, were detected according to the ELISA kit instructions. The results are shown in Table 3. Higher expression levels of IL-6 and TNF-α indicate higher levels of inflammatory factors and a weaker inhibitory effect on inflammation.

[0100] Table 3: IL-6, TNF-α, and the reduction values ​​compared to the model group

[0101]

[0102]

[0103] As shown in Table 3, the product prepared by this invention can effectively inhibit the expression of inflammatory factors IL-6 and TNF-α, and has a good soothing and anti-inflammatory effect, and can inhibit skin irritation caused by inflammatory factors.

[0104] Based on Table 3, products 1 and 6-9 show that when one of the kava pepper extract and mulberry leaf extract is removed from products 6-7 while maintaining the same total amount as in product 1, the anti-inflammatory effect of products 6-7 is weaker than that of product 1. The combination of the two extracts has a synergistic effect, effectively enhancing the anti-inflammatory and soothing effects on the skin. Furthermore, when the ratio of kava pepper extract to mulberry leaf extract is changed in products 8-9, the anti-inflammatory effect is relatively poor. This invention selects a kava pepper extract to mulberry leaf extract mass ratio of 1.5-3:1, resulting in a better anti-inflammatory effect.

[0105] Based on tests of products 1 and 10, it can be seen that the extracts obtained by using 25-40wt% ethanol aqueous solution to extract kava pepper and 10-25wt% ethanol aqueous solution to extract mulberry leaves result in better anti-inflammatory and soothing effects of the product.

[0106] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. However, these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions claimed by the present invention.

Claims

1. A multi-target composition for promoting skin water-locking moisturization, characterized by comprising: According to weight parts, it comprises: hexapeptide-9 0.001-0.1 parts, tripeptide-1 0.001-0.1 parts, carnosine 0.001-0.1 parts, ergothioneine 0.001-0.5 parts, kava-kava root extract 0.1-1.8 parts, oat extract 0.1-1.8 parts, mulberry leaf extract 0.1-1.8 parts, ectoine 0.1-1.5 parts, glycerol glucoside 0.1-2 parts, sodium hyaluronate 0.1-0.5 parts, allantoin 0.001-0.2 parts, butanediol 0.8-2.5 parts and water 20-100 parts. The kava-kava root extract is extracted from kava-kava root using 25-40wt% ethanol solution; The oat extract is extracted from oat kernels using hot water; The mulberry leaf extract is extracted from mulberry leaves using 10-25wt% ethanol solution; The mass ratio of kava-kava root extract and mulberry leaf extract is 1.5-3:

1.

2. The composition of claim 1, wherein, According to weight parts, it comprises: hexapeptide-9 0.001-0.1 parts, tripeptide-1 0.001-0.1 parts, carnosine 0.001-0.1 parts, ergothioneine 0.001-0.5 parts, kava-kava root extract 0.1-1.8 parts, oat extract 0.1-1.8 parts, mulberry leaf extract 0.1-1.8 parts, ectoine 0.1-1.5 parts, glycerol glucoside 0.1-2 parts, sodium hyaluronate 0.1-0.5 parts, allantoin 0.001-0.2 parts, butanediol 0.8-2.5 parts and water 20-100 parts.

3. A process for the preparation of a composition according to any one of claims 1-2, characterized in that, It comprises: Step 1: preparing raw materials; Step 2: water is put into a container, heated, then butanediol is added and stirred, then glycerol glucoside, sodium hyaluronate and allantoin are added and stirred to obtain mixture one; Step 3: mixture one is cooled, hexapeptide-9, tripeptide-1, carnosine, ergothioneine, kava-kava root extract, oat extract, mulberry leaf extract and ectoine are added and stirred, then it is aged, sterilized, filled and packaged to obtain the composition.

4. The production method according to claim 3, characterized by, Step 2 is heated to 40-55℃ and step 3 is cooled to 30-35℃.

5. Use of the composition according to any one of claims 1-2 in the preparation of medical and cosmetic preparations.

6. Use of the composition according to any one of claims 1-2 in the preparation of moisturizing cosmetics.

7. Use of the composition according to any one of claims 1-2 in the preparation of skin soothing cosmetics.

Images